FastQCFastQC Report
Thu 26 May 2016
SRR1364499_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364499_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1444723
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35360.24475280036380678No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29960.20737539306842903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24990.17297433487249805No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22670.1569158932196691No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19450.1346278836842772No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16170.11192456962338108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14800.10244178295770191No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14580.10091899969751987No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG108500.035.8274041
TACATGG108650.034.7724382
CGCATAG3100.034.5906569
ACATGGG108050.030.7266833
AACGCAT4100.029.0558727
ACGCATA3950.028.6542768
CATGGGA66600.027.7386954
CTCAACG3250.027.5046734
GAGTACT44750.026.72031412-13
GTATAGG4250.026.6131671
CATGGGG36550.025.761334
ATGGGAG26050.025.1642235
TACCTGG9800.024.891382
AGTACTT48700.024.67521712-13
GTACTTT50700.024.03095214-15
GTGTACG1501.6812365E-423.8117791
GTATCAA155850.023.6437151
ATGGGTA7250.023.0154045
ATGGGAT24650.021.758265
ATATAGG8950.021.2724782
Produced by FastQC (version 0.11.5)