##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364499_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1444723 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.41994209270566 31.0 26.0 31.0 10.0 34.0 2 26.555001200922252 31.0 26.0 31.0 10.0 34.0 3 26.502472792362273 31.0 26.0 31.0 10.0 34.0 4 29.21677165795796 35.0 28.0 37.0 10.0 37.0 5 29.92297000878369 35.0 30.0 37.0 10.0 37.0 6 30.026037517226484 35.0 30.0 37.0 8.0 37.0 7 29.78991889794791 35.0 30.0 37.0 8.0 37.0 8 29.3964040165485 35.0 28.0 37.0 7.0 37.0 9 29.921122595819405 35.0 27.0 39.0 7.0 39.0 10-11 30.05148945507201 35.0 27.5 39.0 2.0 39.0 12-13 29.91187030316538 35.0 27.0 39.0 2.0 39.0 14-15 30.571718592422215 36.0 27.0 40.0 2.0 41.0 16-17 30.898111956409636 36.0 27.5 40.0 2.0 41.0 18-19 30.91617770326907 36.5 27.5 40.0 2.0 41.0 20-21 30.718168465512072 36.5 27.0 40.0 2.0 41.0 22-23 30.78823864505514 37.0 27.0 40.0 2.0 41.0 24-25 30.83668807100046 37.0 27.5 40.0 2.0 41.0 26-27 30.958280583890478 37.0 28.5 40.0 2.0 41.0 28-29 30.920432844219967 37.0 29.0 40.0 2.0 41.0 30-31 30.563718096825482 36.0 28.0 40.0 2.0 41.0 32-33 30.530519345230886 36.0 28.0 40.0 2.0 41.0 34-35 30.196482993625768 36.0 27.5 40.0 2.0 41.0 36-37 29.924536745106156 35.0 27.0 40.0 2.0 41.0 38-39 29.760575210611307 35.0 26.5 40.0 2.0 41.0 40-41 29.77671602099503 35.0 27.0 40.0 2.0 41.0 42-43 29.613186403206704 35.0 26.5 40.0 2.0 41.0 44-45 29.20717535472198 35.0 25.5 40.0 2.0 41.0 46-47 28.866605916843575 35.0 24.5 40.0 2.0 41.0 48-49 28.542029856242337 35.0 24.0 39.5 2.0 41.0 50-51 28.503319321420094 35.0 24.0 39.0 2.0 41.0 52-53 28.299344926328438 34.5 23.0 39.0 2.0 41.0 54-55 28.15681725839486 34.0 23.0 39.0 2.0 41.0 56-57 27.71638819344608 34.0 22.0 39.0 2.0 40.5 58-59 27.46778413578243 33.5 20.5 39.0 2.0 40.0 60-61 27.045686958676505 33.0 18.5 38.5 2.0 40.0 62-63 27.005359158814528 33.0 19.5 38.0 2.0 40.0 64-65 26.819155990456302 33.0 19.0 38.0 2.0 40.0 66-67 26.5360660832561 33.0 18.0 37.5 2.0 40.0 68-69 26.344671608329072 33.0 17.0 37.0 2.0 40.0 70-71 26.16046051734485 33.0 14.5 37.0 2.0 39.5 72-73 26.119978016547115 33.0 15.5 36.0 2.0 39.0 74-75 25.8340726907511 32.5 13.0 36.0 2.0 39.0 76-77 25.528793062753206 32.0 11.0 35.5 2.0 39.0 78-79 25.18922935400073 32.0 8.0 35.0 2.0 37.5 80-81 25.14847586700011 32.0 7.5 35.0 2.0 37.0 82-83 24.930948354805732 32.0 7.0 35.0 2.0 37.0 84-85 24.73498726053368 32.0 4.0 35.0 2.0 36.0 86-87 24.513114278654108 32.0 2.0 35.0 2.0 36.0 88-89 24.26119124565747 32.0 2.0 35.0 2.0 36.0 90-91 24.06599881084471 32.0 2.0 35.0 2.0 35.0 92-93 23.940768576398384 32.0 2.0 35.0 2.0 35.0 94-95 23.878107775677414 32.0 2.0 35.0 2.0 35.0 96-97 23.73435738200333 32.0 2.0 35.0 2.0 35.0 98-99 23.63154805454056 32.0 2.0 35.0 2.0 35.0 100-101 23.563488294988034 32.0 2.0 35.0 2.0 35.0 102-103 22.43889036168179 29.5 2.0 34.0 2.0 35.0 104-105 23.164337385090427 31.0 2.0 34.5 2.0 35.0 106-107 23.2306123734446 31.5 2.0 35.0 2.0 35.0 108-109 23.18207123441656 31.5 2.0 35.0 2.0 35.0 110-111 23.075334164403834 31.0 2.0 35.0 2.0 35.0 112-113 23.026151725970998 31.5 2.0 35.0 2.0 35.0 114-115 22.95494049724411 32.0 2.0 35.0 2.0 35.0 116-117 22.743025479624812 31.0 2.0 35.0 2.0 35.0 118-119 22.60083801531505 31.0 2.0 35.0 2.0 35.0 120-121 22.458500003114786 31.0 2.0 35.0 2.0 35.0 122-123 22.18154898897574 31.0 2.0 35.0 2.0 35.0 124-125 21.234878243095736 29.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.9615049873785857 1101 2 1.6031084975858718 1101 3 1.9679928621564713 1101 4 2.1920347402846794 1101 5 1.8602037749880367 1101 6 1.7803609177347433 1101 7 1.624433618886954 1101 8 2.0022613776340883 1101 9 2.5032771841202113 1101 10-11 2.387082110376177 1101 12-13 2.2303450308702217 1101 14-15 2.6916932392432464 1101 16-17 2.3101429159950477 1101 18-19 2.263186943303584 1101 20-21 2.7020077948375025 1101 22-23 2.4743580744893663 1101 24-25 2.4991901465009327 1101 26-27 2.2182834919773917 1101 28-29 2.086602189649888 1101 30-31 2.2256562187784077 1101 32-33 2.3833552569823233 1101 34-35 2.662774788161716 1101 36-37 2.772189111928281 1101 38-39 2.6788408432354096 1101 40-41 2.5322227907800077 1101 42-43 2.5560035571841198 1101 44-45 2.6020233664305508 1101 46-47 2.4612020267432513 1101 48-49 2.459514978489313 1101 50-51 2.4963804768441094 1101 52-53 2.344225331111982 1101 54-55 2.283878290124033 1101 56-57 2.6288927641718445 1101 58-59 2.4418468178671198 1101 60-61 2.519617141024341 1101 62-63 2.343291050764968 1101 64-65 2.3401813780921046 1101 66-67 2.4878252792036655 1101 68-69 2.35647982629677 1101 70-71 2.29630222233115 1101 72-73 2.18474871399901 1101 74-75 2.2165833326000275 1101 76-77 2.2987535560695953 1101 78-79 2.218960926932141 1101 80-81 2.166943750546526 1101 82-83 2.1985310315671107 1101 84-85 2.035795712240173 1101 86-87 1.9906167349575696 1101 88-89 2.0926912318935926 1101 90-91 1.9653001132375465 1101 92-93 2.0153832845935256 1101 94-95 2.055643002471175 1101 96-97 2.0989139070449063 1101 98-99 1.9101576305569026 1101 100-101 1.9558686704546346 1101 102-103 1.841098080192225 1101 104-105 1.8300784383918867 1101 106-107 1.9602329823410365 1101 108-109 1.9250728520320344 1101 110-111 2.0001866305134364 1101 112-113 1.9149242633830283 1101 114-115 1.8914860442084667 1101 116-117 1.9747821086980046 1101 118-119 1.8748065677877896 1101 120-121 1.8322012915921313 1101 122-123 1.9469718035802828 1101 124-125 2.074063287696127 1102 1 1.0135867367261078 1102 2 0.7355260627731148 1102 3 0.9706694407037268 1102 4 1.3811742333973847 1102 5 1.0786603924641156 1102 6 1.0426715321999431 1102 7 0.910721217656171 1102 8 1.1285978178970417 1102 9 1.4753785221333686 1102 10-11 1.404174365062751 1102 12-13 1.672390034705316 1102 14-15 1.8615460224924583 1102 16-17 1.4121320767493195 1102 18-19 1.4876115755575476 1102 20-21 1.6619168057617593 1102 22-23 1.6653531854092947 1102 24-25 1.3437110298684978 1102 26-27 1.4693160288456717 1102 28-29 1.0928985887237843 1102 30-31 1.206408954475986 1102 32-33 1.0412542601732717 1102 34-35 1.5452880581415371 1102 36-37 1.6688402626834922 1102 38-39 1.563963535694736 1102 40-41 1.2005841373646824 1102 42-43 1.304886606815117 1102 44-45 1.6807318556478137 1102 46-47 1.6437187087641014 1102 48-49 1.6520323922253262 1102 50-51 1.5237569782161238 1102 52-53 1.5273065777258275 1102 54-55 1.3792108152178209 1102 56-57 1.6049388555210626 1102 58-59 1.6067072722918176 1102 60-61 2.001804511778495 1102 62-63 1.6644556257321454 1102 64-65 1.6374520016613374 1102 66-67 1.7279109825742331 1102 68-69 1.5201168766444901 1102 70-71 1.7479722389120909 1102 72-73 1.5632280836747228 1102 74-75 1.5566385839974366 1102 76-77 1.4760784133407654 1102 78-79 1.512382748492648 1102 80-81 1.0766289966641445 1102 82-83 1.0226055055753456 1102 84-85 1.100942718943351 1102 86-87 1.1316810629758116 1102 88-89 0.8082893281719201 1102 90-91 0.9381534808727388 1102 92-93 0.9115315044449375 1102 94-95 0.9258876182013971 1102 96-97 1.0873666134936038 1102 98-99 1.3124063036575393 1102 100-101 1.2230387808174008 1102 102-103 1.0155488352690156 1102 104-105 0.9463966922789027 1102 106-107 1.2112585176222304 1102 108-109 1.2921085713681855 1102 110-111 0.970968613122281 1102 112-113 0.9100502712417722 1102 114-115 1.07017315393162 1102 116-117 1.260382565793165 1102 118-119 1.3895650258529173 1102 120-121 1.3188246115317277 1102 122-123 1.436504825333131 1102 124-125 1.3857535319607877 1103 1 1.6453664521699842 1103 2 1.4488639963209238 1103 3 1.5434236249572528 1103 4 2.155485321407852 1103 5 1.6711088715555427 1103 6 1.5927603999796212 1103 7 1.584984809945734 1103 8 1.8294195834832543 1103 9 2.5101095440082837 1103 10-11 2.138098557112741 1103 12-13 2.1514506948062184 1103 14-15 2.42208365015588 1103 16-17 2.285598099385961 1103 18-19 2.0512543479933036 1103 20-21 2.3867734971321717 1103 22-23 2.199142656892441 1103 24-25 2.31581998923426 1103 26-27 2.1037698429910385 1103 28-29 1.958630575448879 1103 30-31 2.3212742189733824 1103 32-33 2.277895565917266 1103 34-35 2.0877997155032197 1103 36-37 2.4151046794270776 1103 38-39 2.525210910734689 1103 40-41 2.4122143159665193 1103 42-43 2.506050998765339 1103 44-45 2.431193520029307 1103 46-47 2.6260022074363114 1103 48-49 2.5713273048063137 1103 50-51 2.4749125580671816 1103 52-53 2.578767145822443 1103 54-55 2.384381277061429 1103 56-57 2.582816902042971 1103 58-59 2.82856964223868 1103 60-61 2.6894765344091596 1103 62-63 2.587822624271425 1103 64-65 2.428664994578803 1103 66-67 2.45774606189806 1103 68-69 2.3843463257837456 1103 70-71 2.429930436703387 1103 72-73 2.3346948053049914 1103 74-75 2.4334603734808695 1103 76-77 2.488125000967379 1103 78-79 2.5194551301790185 1103 80-81 2.1418474919160673 1103 82-83 2.1954304853373863 1103 84-85 2.3152437103791854 1103 86-87 2.0949830013389104 1103 88-89 2.204615429609639 1103 90-91 2.1409667808694515 1103 92-93 2.115003172799348 1103 94-95 2.0799401383123985 1103 96-97 2.186045993480281 1103 98-99 2.1363719275093516 1103 100-101 2.0216142079054933 1103 102-103 2.09240467828819 1103 104-105 2.310414651038279 1103 106-107 2.0421725563417006 1103 108-109 2.2763235626905107 1103 110-111 2.4892810513255696 1103 112-113 2.3645759562704605 1103 114-115 2.308952364546766 1103 116-117 2.407711006171102 1103 118-119 2.432314161819125 1103 120-121 2.4209655035551343 1103 122-123 2.4830013438071745 1103 124-125 2.4267178444527637 1104 1 1.5500761172050161 1104 2 1.3230438600314542 1104 3 1.3214205536452255 1104 4 1.6422758003405704 1104 5 1.1156563329241962 1104 6 1.0400624443216913 1104 7 1.0810852034575902 1104 8 1.4713785046849068 1104 9 1.7369297762762557 1104 10-11 1.7583236266971554 1104 12-13 1.2321047882892806 1104 14-15 1.5271508351064504 1104 16-17 1.2469259619447435 1104 18-19 0.8279469288551908 1104 20-21 -3.6963899542547196 1104 22-23 -0.3575249695592291 1104 24-25 1.0630011006653035 1104 26-27 1.1538734997719224 1104 28-29 0.8411303355026263 1104 30-31 1.5362694200483382 1104 32-33 1.3847042807651455 1104 34-35 1.6265423971425363 1104 36-37 1.7837177900902894 1104 38-39 1.6435667990157121 1104 40-41 1.6857237698488454 1104 42-43 1.6652299026908608 1104 44-45 1.6952068610409228 1104 46-47 1.6960727516344143 1104 48-49 1.7461886158726365 1104 50-51 1.5171536560612324 1104 52-53 1.3506564826110043 1104 54-55 1.4813622733182683 1104 56-57 1.7215845380924932 1104 58-59 1.8100769845939908 1104 60-61 1.9428679347354887 1104 62-63 1.582792391043668 1104 64-65 1.648829597707703 1104 66-67 1.6362972664282438 1104 68-69 1.6590780658739668 1104 70-71 1.8367333928412144 1104 72-73 1.6676111120522812 1104 74-75 1.771703007130931 1104 76-77 1.7220629988558542 1104 78-79 1.6477049622166398 1104 80-81 1.4298876109294092 1104 82-83 1.4508096004156137 1104 84-85 1.3201366943507793 1104 86-87 1.163560120064318 1104 88-89 1.4797641962859025 1104 90-91 1.498116219395225 1104 92-93 1.1503870868026098 1104 94-95 1.3616720703596208 1104 96-97 1.3491984073395784 1104 98-99 1.266889251628747 1104 100-101 0.7903276161020578 1104 102-103 1.1447201596203733 1104 104-105 1.34878397630942 1104 106-107 1.512007473320299 1104 108-109 1.4728875323613018 1104 110-111 1.4655940372167322 1104 112-113 1.4019303088954729 1104 114-115 1.3863715026598236 1104 116-117 1.5221716069965119 1104 118-119 1.4840000241859563 1104 120-121 1.445953026350029 1104 122-123 1.354780305319796 1104 124-125 1.4631954334728228 1105 1 1.3695918533819231 1105 2 1.0889071921501312 1105 3 1.2293116566160158 1105 4 1.6209618631318499 1105 5 1.2515098838725862 1105 6 1.2472514126959062 1105 7 1.0080998368554894 1105 8 1.4234080016258943 1105 9 1.990976303960803 1105 10-11 1.7162324700258225 1105 12-13 1.772693480489277 1105 14-15 1.93840867442675 1105 16-17 1.622652837148518 1105 18-19 1.4183069142793308 1105 20-21 1.7475202124087552 1105 22-23 1.718127204353813 1105 24-25 1.5596290901553438 1105 26-27 1.390889092108086 1105 28-29 0.9726327040801408 1105 30-31 1.5467953976859015 1105 32-33 1.6128671468398217 1105 34-35 1.3246352607239764 1105 36-37 1.607796346312842 1105 38-39 1.5600327279392125 1105 40-41 1.677380911062098 1105 42-43 1.8548596566273048 1105 44-45 1.962159067557863 1105 46-47 2.3800865795241712 1105 48-49 1.9622638733475561 1105 50-51 2.104823819656101 1105 52-53 1.9160812922617545 1105 54-55 1.9784844036955143 1105 56-57 2.15244739074204 1105 58-59 1.992164497299921 1105 60-61 2.2869634641653036 1105 62-63 2.1078032270460483 1105 64-65 1.924439866018627 1105 66-67 2.1061717414095433 1105 68-69 1.6558412656591628 1105 70-71 1.703996774824681 1105 72-73 1.738134404662702 1105 74-75 1.8399247520149942 1105 76-77 1.6486658641633873 1105 78-79 1.3679641632292778 1105 80-81 1.4832235653606638 1105 82-83 1.4994602783849622 1105 84-85 1.4217281482929742 1105 86-87 1.4093878755734153 1105 88-89 1.5564231594818487 1105 90-91 1.6103396136530996 1105 92-93 1.588262559079702 1105 94-95 1.343100600690839 1105 96-97 1.5255507020788421 1105 98-99 1.4085236660051486 1105 100-101 1.4833834646665274 1105 102-103 1.5745100562468295 1105 104-105 1.6088455401459356 1105 106-107 1.6383190131833594 1105 108-109 1.8517813011804662 1105 110-111 1.6266247999562253 1105 112-113 1.7585746962298145 1105 114-115 1.6265228231178135 1105 116-117 1.728474995884337 1105 118-119 1.7033363430137491 1105 120-121 1.7432976304044203 1105 122-123 1.7973775896066329 1105 124-125 1.5432506683168015 1106 1 -0.1924274226012841 1106 2 -0.08931204625335454 1106 3 -0.053947114235988636 1106 4 -0.45091021412071797 1106 5 -0.8418686345095843 1106 6 -0.3742863257136122 1106 7 -0.24053253521974938 1106 8 -0.006940323308825214 1106 9 0.3313748290429679 1106 10-11 -2.2943973044134935 1106 12-13 -0.40660813813938645 1106 14-15 -0.08674493411706052 1106 16-17 -0.329487128730932 1106 18-19 -3.1054737511651958 1106 20-21 -5.487111319581608 1106 22-23 -6.186779439154655 1106 24-25 -5.983533287372982 1106 26-27 -5.169748430036083 1106 28-29 -1.6943812845015245 1106 30-31 -3.5580684945809686 1106 32-33 -4.233728009819917 1106 34-35 -3.5942595813731977 1106 36-37 -6.028096289843258 1106 38-39 -6.2405814763906555 1106 40-41 -2.504624263906745 1106 42-43 -1.15754634379293 1106 44-45 -0.4279992595201634 1106 46-47 -0.3493880839486643 1106 48-49 -0.17734363884238746 1106 50-51 -0.2132473331966338 1106 52-53 -0.4335506007773944 1106 54-55 -0.41097088381137326 1106 56-57 -0.28618264648716263 1106 58-59 -0.16729610155073615 1106 60-61 0.12436777426426815 1106 62-63 -0.04976986234982661 1106 64-65 -0.2048517064513362 1106 66-67 -0.5224366156537279 1106 68-69 -0.33575721513945567 1106 70-71 -0.09965732625863666 1106 72-73 -0.21921146341639286 1106 74-75 -0.20911879430009606 1106 76-77 -0.369462232883464 1106 78-79 -0.2737148304923025 1106 80-81 -0.5439572794781888 1106 82-83 -0.6767035676638322 1106 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-4.6958053053274895 2216 78-79 -4.99568201075337 2216 80-81 -4.859718361620256 2216 82-83 -5.092746972495913 2216 84-85 -4.776470210267529 2216 86-87 -5.023729296387607 2216 88-89 -5.215416917444745 2216 90-91 -5.209017212627732 2216 92-93 -5.000337459499271 2216 94-95 -7.890496268547846 2216 96-97 -9.917376057634744 2216 98-99 -6.732616934748428 2216 100-101 -5.7100538923870765 2216 102-103 -6.749472230313284 2216 104-105 -9.299109914071998 2216 106-107 -6.004086793543749 2216 108-109 -8.267991901818203 2216 110-111 -5.778721531984797 2216 112-113 -5.289218420018756 2216 114-115 -5.646853820365926 2216 116-117 -5.174129268416976 2216 118-119 -5.38936922120396 2216 120-121 -6.072756606853545 2216 122-123 -8.921418956335632 2216 124-125 -7.393267457402043 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 132764.0 3 19692.0 4 11573.0 5 9981.0 6 10506.0 7 11419.0 8 12343.0 9 13355.0 10 14435.0 11 15550.0 12 16982.0 13 18378.0 14 20602.0 15 17438.0 16 13292.0 17 11441.0 18 10454.0 19 9705.0 20 9478.0 21 9964.0 22 11697.0 23 15717.0 24 21190.0 25 25689.0 26 31255.0 27 34039.0 28 38611.0 29 44125.0 30 48502.0 31 54323.0 32 63621.0 33 77728.0 34 95182.0 35 118808.0 36 153798.0 37 169188.0 38 51653.0 39 245.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.8047856821679 19.228432248101115 13.707653114600992 21.259128955129984 2 14.7693841589437 21.362404234621284 40.68442170347265 23.183789902962364 3 19.463279183458667 28.14616311437949 28.708643206337335 23.681914495824504 4 10.593367935209775 17.255002441059663 38.90502027291393 33.246609350816634 5 12.4044407232969 40.907646057926634 32.87751584315502 13.810397375621452 6 29.43954603284035 37.99836088949433 18.00266632333947 14.559426754325855 7 27.59663265263744 35.16734985443325 20.251706585044072 16.98431090788525 8 25.843706911350207 35.154523858306526 19.744915193230963 19.256854037112305 9 26.46968225499758 18.03676879297324 20.741301174195254 34.75224777783392 10-11 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27.818481270124344 28.771296064888052 21.46284036146455 36-37 21.94738547071495 27.45034249419947 29.04150769435231 21.560764340733275 38-39 21.601445091906903 28.003189779415123 29.018720571723655 21.376644556954314 40-41 21.478187808519795 28.104013340760254 29.116236947355993 21.301561903363957 42-43 21.298733304443058 28.29072248305004 28.44495779296748 21.965586419539427 44-45 21.546537520602058 28.170274069282502 28.273141432056438 22.010046978059005 46-47 21.854050218907368 28.415318324190565 27.762680187585442 21.96795126931663 48-49 21.943478405334012 28.482378708251883 27.686873189830376 21.88726969658373 50-51 21.61025968681415 28.769965225800398 27.746640864928175 21.87313422245727 52-53 21.959534881306126 28.974942500488897 27.352184289177977 21.713338329027 54-55 21.426304376794388 28.588665087229575 27.65207850575329 22.332952030222742 56-57 21.836284196580237 28.913856666818948 27.275134252856375 21.974724883744436 58-59 22.20880003322857 28.24902737203539 27.327419109197393 22.214753485538647 60-61 21.88276780805699 28.466545543675593 27.98924294899402 21.6614436992734 62-63 21.7676453730649 28.536446753074145 27.777739321126273 21.91816855273468 64-65 21.495078680882965 28.609688412212652 27.667026850418065 22.228206056486318 66-67 21.536481254138184 28.81591111329545 27.26779362540672 22.37981400715964 68-69 21.4569991634597 28.680907118414584 27.471125169203 22.390968548922725 70-71 21.525424315419333 28.69040202674641 27.623037627709945 22.161136030124318 72-73 21.764249043580342 28.45825220407403 27.622410295207832 22.155088457137794 74-75 21.326185404311946 29.175594367718034 27.31002642403393 22.18819380393609 76-77 21.788515797838613 28.483491943946817 27.49434456482915 22.233647693385425 78-79 21.778829028884676 28.609995801072614 27.56670254043761 22.044472629605103 80-81 21.672195544019036 29.00710361237292 27.35394332684404 21.96675751676401 82-83 21.752276212656252 28.88868353626902 27.298942768570946 22.060097482503785 84-85 21.586489309493437 28.768845699397726 27.21650897457107 22.42815601653777 86-87 21.74237321924109 28.64227970365735 27.41560581970112 22.199741257400433 88-89 21.8499604576187 28.417802897236534 27.181034885552062 22.551201759592704 90-91 21.61964604312065 28.60125253734856 27.53052172904838 22.248579690482412 92-93 21.832184817440137 28.718088753265835 27.418052517130832 22.031673912163193 94-95 21.63788168566399 28.387487306851288 27.353796038837746 22.62083496864697 96-97 21.60037156451642 28.33812786304719 27.49068666064005 22.570813911796343 98-99 21.504761931131103 28.501156305829568 27.093377319793145 22.900704443246184 100-101 21.433137409015977 28.41373290680599 27.396283148535964 22.756846535642065 102-103 21.961948256994276 28.055286952392755 27.48386016084811 22.49890462976486 104-105 21.860739581945328 27.9563982715746 27.617496759635003 22.56536538684507 106-107 21.375906494923214 28.528330540391067 27.795486023867717 22.300276940818005 108-109 21.4107971991572 28.643227558786965 27.278557327847565 22.66741791420827 110-111 21.068244123214104 28.818426948092963 27.40151250598312 22.71181642270981 112-113 21.526246277676023 28.529579929422937 27.289359033462496 22.65481475943854 114-115 21.1826652988954 28.791965866631227 27.238894818666477 22.786474015806895 116-117 21.191168235329137 28.848287715562744 27.317915978206468 22.64262807090165 118-119 21.34048420679573 28.760542185354183 27.263074247995394 22.635899359854694 120-121 20.945083942315744 29.33869251700963 27.056747677421722 22.659475863252904 122-123 21.06558128829844 28.871445307970923 27.096907052363623 22.96606635136702 124-125 21.237622819547244 28.88604943080985 27.1003419469333 22.775985802709606 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2024.0 1 2082.5 2 2497.0 3 2451.0 4 1645.0 5 1075.5 6 939.0 7 1129.0 8 1474.5 9 1653.0 10 1495.5 11 1318.0 12 1360.0 13 1473.0 14 1650.0 15 1866.5 16 1987.0 17 2188.0 18 2465.0 19 2740.0 20 3178.5 21 3690.0 22 4411.5 23 5392.5 24 6448.0 25 7903.0 26 10030.0 27 12246.5 28 14086.0 29 16188.5 30 18900.0 31 21955.5 32 25704.0 33 29529.0 34 33226.5 35 36108.5 36 39570.5 37 43112.0 38 43956.5 39 44068.0 40 43883.5 41 44368.0 42 45417.0 43 46410.5 44 47712.5 45 48570.5 46 49386.5 47 52465.5 48 60202.0 49 61381.0 50 54502.5 51 48965.0 52 45290.5 53 43416.5 54 40907.0 55 37096.5 56 33469.0 57 29982.5 58 27035.0 59 24612.5 60 21393.5 61 17676.5 62 14324.0 63 11319.5 64 8683.5 65 6828.5 66 5355.5 67 4241.0 68 3320.5 69 2497.5 70 1909.0 71 1559.0 72 1405.0 73 1261.5 74 1045.0 75 783.0 76 555.0 77 395.0 78 279.5 79 186.5 80 120.0 81 80.0 82 58.0 83 37.0 84 28.0 85 26.0 86 20.5 87 15.5 88 9.5 89 4.5 90 4.0 91 4.5 92 3.5 93 2.0 94 0.5 95 0.5 96 1.5 97 2.0 98 0.5 99 0.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09524317118229585 2 0.030317230361806377 3 0.040353756394824476 4 0.04692941138197426 5 0.05011341274417311 6 0.002561044573942548 7 0.0017304355229341543 8 0.0018688703647688864 9 0.005122089147885096 10-11 0.06496054953094815 12-13 0.005122089147885096 14-15 0.009205916982009699 16-17 0.017892703307139154 18-19 0.015573919706407386 20-21 0.12884822903767712 22-23 0.013289744816134303 24-25 0.012666788027878009 26-27 0.005675828515224026 28-29 0.004049219123665921 30-31 0.0015227832601820558 32-33 0.00515669785834378 34-35 0.018411833964019398 36-37 0.0018688703647688864 38-39 0.007544698879992913 40-41 0.02959044744217404 42-43 0.012251483502373811 44-45 0.11092091702007928 46-47 0.003322436204033576 48-49 0.00740626403815818 50-51 0.008133046957790524 52-53 0.010071134743476777 54-55 0.007994612115955793 56-57 0.004222262675959336 58-59 0.012666788027878009 60-61 0.03190923104290581 62-63 0.006575654987149786 64-65 0.006229567882562955 66-67 0.006748698539443201 68-69 0.005710437225682709 70-71 0.002976349099446745 72-73 8.30609051008394E-4 74-75 0.0010728700242191756 76-77 0.006575654987149786 78-79 0.02121513951117273 80-81 0.002457218442566499 82-83 0.00370313201907909 84-85 0.002076522627520985 86-87 0.0030455665203641116 88-89 0.004879828174674315 90-91 0.003841566860913822 92-93 0.0022495661798144005 94-95 0.003184001362198844 96-97 0.0014535658392646896 98-99 0.0024918271530251818 100-101 0.0022495661798144005 102-103 4.15304525504197E-4 104-105 0.003841566860913822 106-107 6.921742091736617E-4 108-109 0.0010382613137604926 110-111 0.002388001021649133 112-113 0.004222262675959336 114-115 0.002561044573942548 116-117 0.005122089147885096 118-119 0.003530088466785674 120-121 0.006921742091736617 122-123 0.004533741070087484 124-125 0.05437028413059112 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1444723.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.44943449442818 #Duplication Level Percentage of deduplicated Percentage of total 1 84.65189441468164 53.71114829493593 2 8.592071395208423 10.903241423234537 3 2.776451754442658 5.284928811613489 4 1.2684971631276483 3.219417106329428 5 0.6888835546375136 2.185463598713088 6 0.42486725031261896 1.6174552060523002 7 0.2725582078277469 1.2105564907439759 8 0.20920324567647391 1.0619062106056958 9 0.1369524881240448 0.7820602131667982 >10 0.8667266381284695 10.389374613463827 >50 0.07124347850483162 3.168921580209836 >100 0.03779578344966419 4.261097631750048 >500 0.0017127755269824966 0.7308007197252078 >1k 0.0011418503513216643 1.473628099455807 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3536 0.24475280036380678 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2996 0.20737539306842903 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2499 0.17297433487249805 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2267 0.1569158932196691 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1945 0.1346278836842772 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1617 0.11192456962338108 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1480 0.10244178295770191 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1458 0.10091899969751987 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.4608710458683086E-5 0.0 12-13 0.0 0.0 0.0 6.921742091736617E-5 0.0 14-15 0.0 0.0 0.0 6.921742091736617E-5 0.0 16-17 0.0 0.0 0.0 1.0382613137604926E-4 0.0 18-19 0.0 0.0 0.0 2.7686968366946464E-4 0.0 20-21 0.0 0.0 0.0 5.537393673389293E-4 0.0 22-23 0.0 0.0 0.0 0.0010036526033018094 0.0 24-25 0.0 0.0 0.0 0.0016266093915581047 0.0 26-27 0.0 0.0 0.0 0.002214957469355717 0.0 28-29 0.0 0.0 0.0 0.0038761755713725052 0.0 30-31 0.0 0.0 0.0 0.007475481459075546 3.4608710458683086E-5 32-33 0.0 0.0 0.0 0.013532005789345086 6.921742091736617E-5 34-35 0.0 0.0 0.0 0.022530270508602686 6.921742091736617E-5 36-37 0.0 0.0 0.0 0.036442972112993285 6.921742091736617E-5 38-39 0.0 0.0 0.0 0.06392228821718765 6.921742091736617E-5 40-41 0.0 0.0 0.0 0.09468943181495693 6.921742091736617E-5 42-43 0.0 0.0 0.0 0.12618335833235853 1.0382613137604926E-4 44-45 0.0 0.0 0.0 0.15823102421709906 1.3843484183473235E-4 46-47 0.0 0.0 0.0 0.20419139170623019 1.3843484183473235E-4 48-49 0.0 0.0 0.0 0.2562775009465482 1.3843484183473235E-4 50-51 0.0 0.0 0.0 0.3175003097479586 1.3843484183473235E-4 52-53 0.0 0.0 0.0 0.37470850813616174 1.3843484183473235E-4 54-55 0.0 0.0 0.0 0.4369349695408739 1.3843484183473235E-4 56-57 0.0 0.0 0.0 0.5133163935231875 1.3843484183473235E-4 58-59 0.0 0.0 0.0 0.6010148658254905 1.3843484183473235E-4 60-61 0.0 0.0 0.0 0.702626039732184 1.3843484183473235E-4 62-63 0.0 0.0 0.0 0.8047909530062164 1.3843484183473235E-4 64-65 0.0 0.0 0.0 0.9066789965965794 1.3843484183473235E-4 66-67 0.0 0.0 0.0 1.0242793947351845 1.3843484183473235E-4 68-69 0.0 0.0 0.0 1.1645484982242271 1.3843484183473235E-4 70-71 0.0 0.0 0.0 1.33108561295141 1.3843484183473235E-4 72-73 0.0 0.0 0.0 1.5012566422767548 1.3843484183473235E-4 74-75 0.0 0.0 0.0 1.66668627826926 1.3843484183473235E-4 76-77 0.0 0.0 0.0 1.8395913957208405 1.3843484183473235E-4 78-79 0.0 0.0 0.0 2.0632328827048507 1.3843484183473235E-4 80-81 0.0 0.0 0.0 2.3163955997101175 1.3843484183473235E-4 82-83 0.0 0.0 0.0 2.578279711750972 1.3843484183473235E-4 84-85 0.0 0.0 0.0 2.828950601603214 1.3843484183473235E-4 86-87 0.0 6.921742091736617E-5 0.0 3.089138886831593 1.3843484183473235E-4 88-89 0.0 6.921742091736617E-5 0.0 3.384420404465077 1.3843484183473235E-4 90-91 0.0 6.921742091736617E-5 0.0 3.7341068149396115 1.3843484183473235E-4 92-93 0.0 1.0382613137604926E-4 3.4608710458683086E-5 4.106773409158711 1.3843484183473235E-4 94-95 0.0 1.3843484183473235E-4 6.921742091736617E-5 4.464835127564246 1.3843484183473235E-4 96-97 0.0 1.3843484183473235E-4 6.921742091736617E-5 4.809503275022271 1.3843484183473235E-4 98-99 0.0 1.3843484183473235E-4 6.921742091736617E-5 5.192587091089433 1.3843484183473235E-4 100-101 0.0 1.3843484183473235E-4 6.921742091736617E-5 5.645061371626257 1.3843484183473235E-4 102-103 0.0 1.3843484183473235E-4 6.921742091736617E-5 6.139308365686709 1.3843484183473235E-4 104-105 0.0 1.3843484183473235E-4 6.921742091736617E-5 6.626149095709005 1.3843484183473235E-4 106-107 0.0 1.3843484183473235E-4 6.921742091736617E-5 7.09589312276471 1.3843484183473235E-4 108-109 0.0 1.3843484183473235E-4 6.921742091736617E-5 7.575119936486095 1.3843484183473235E-4 110-111 0.0 2.076522627520985E-4 6.921742091736617E-5 8.13557339365401 1.3843484183473235E-4 112-113 0.0 2.076522627520985E-4 6.921742091736617E-5 8.743440784150318 1.3843484183473235E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10850 0.0 35.827404 1 TACATGG 10865 0.0 34.772438 2 CGCATAG 310 0.0 34.590656 9 ACATGGG 10805 0.0 30.726683 3 AACGCAT 410 0.0 29.055872 7 ACGCATA 395 0.0 28.654276 8 CATGGGA 6660 0.0 27.738695 4 CTCAACG 325 0.0 27.504673 4 GAGTACT 4475 0.0 26.720314 12-13 GTATAGG 425 0.0 26.613167 1 CATGGGG 3655 0.0 25.76133 4 ATGGGAG 2605 0.0 25.164223 5 TACCTGG 980 0.0 24.89138 2 AGTACTT 4870 0.0 24.675217 12-13 GTACTTT 5070 0.0 24.030952 14-15 GTGTACG 150 1.6812365E-4 23.811779 1 GTATCAA 15585 0.0 23.643715 1 ATGGGTA 725 0.0 23.015404 5 ATGGGAT 2465 0.0 21.75826 5 ATATAGG 895 0.0 21.272478 2 >>END_MODULE