FastQCFastQC Report
Thu 26 May 2016
SRR1364499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364499_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1444723
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77550.5367810992141746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77280.5349122288494057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44960.31120152444447824No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38550.26683315763644655No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22770.15760806742884276No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22420.15518545769673495No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17130.11856944203144824No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA16760.11600839745750569No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14660.10147273906485879No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14520.10050369517201568No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14470.10015760806742884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG100100.031.1116961
TACATGG99600.029.8580382
ACATGGG100450.027.706843
GAGTACT97350.026.69516612-13
AGTACTT100150.025.1175112-13
GTACTTT106900.024.8114314-15
GTATCAA196700.024.1129341
CATGGGG33650.024.0865124
ACCGCAG4200.024.0797586
GGTATCA155700.023.488711
CATGGGA63850.022.8678464
CACCGCA5000.022.6066465
TCACCGC4550.022.2667884
ACTTTTT122950.022.22588716-17
CCGCAGA4600.021.9858677
TACTTTT116950.021.66227314-15
CGTATAC1400.003126552621.284433
ATGGGAT23300.020.681485
GTATCAC6700.020.480491
TATCAAC230150.020.404942
Produced by FastQC (version 0.11.5)