##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364499_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1444723 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.62558358938011 31.0 28.0 31.0 23.0 34.0 2 28.754667849823115 31.0 28.0 33.0 23.0 34.0 3 28.87534150145045 31.0 28.0 33.0 24.0 34.0 4 32.24952949458132 35.0 32.0 37.0 25.0 37.0 5 31.337410700874837 35.0 32.0 37.0 17.0 37.0 6 31.369855674755645 35.0 32.0 37.0 17.0 37.0 7 30.811058590470285 35.0 30.0 37.0 11.0 37.0 8 30.909739790949544 35.0 31.0 37.0 13.0 37.0 9 31.39052468881578 37.0 30.0 39.0 10.0 39.0 10-11 31.587557614850738 37.0 30.0 39.0 10.0 39.0 12-13 31.319344261841195 37.0 30.0 39.0 10.0 39.0 14-15 32.19565722979422 37.0 30.5 40.0 10.0 41.0 16-17 32.29172789524359 37.0 31.0 40.0 10.0 41.0 18-19 32.23440479593666 37.0 30.0 40.0 10.0 41.0 20-21 32.1506402957522 37.0 30.0 40.0 9.0 41.0 22-23 32.12061447073245 37.0 30.0 40.0 8.0 41.0 24-25 31.90381477971902 37.0 30.0 40.0 8.0 41.0 26-27 31.967993172393598 37.0 30.0 40.0 7.0 41.0 28-29 31.83860227877593 37.0 30.0 40.0 2.0 41.0 30-31 31.25863781499983 36.5 29.5 40.0 2.0 41.0 32-33 31.09321233205258 36.0 30.0 40.0 2.0 41.0 34-35 30.813671548109916 36.0 29.0 40.0 2.0 41.0 36-37 30.727234217216726 36.0 29.0 40.0 2.0 41.0 38-39 30.454976836390088 35.0 28.0 40.0 2.0 41.0 40-41 30.13703422732247 35.0 27.5 40.0 2.0 41.0 42-43 29.962054663765997 35.0 27.0 40.0 2.0 41.0 44-45 29.78464660699664 35.0 27.0 40.0 2.0 41.0 46-47 29.467639125285608 35.0 26.0 40.0 2.0 41.0 48-49 29.04167546304724 35.0 24.5 40.0 2.0 41.0 50-51 28.654952541075346 35.0 23.5 40.0 2.0 41.0 52-53 28.549050233158884 35.0 23.5 39.0 2.0 41.0 54-55 28.079233873898318 34.5 22.0 39.0 2.0 41.0 56-57 28.075955044669463 34.0 22.5 39.0 2.0 41.0 58-59 27.82496263989706 34.0 22.0 39.0 2.0 41.0 60-61 27.516210373891745 34.0 21.0 39.0 2.0 40.5 62-63 27.262662115851967 34.0 20.0 38.0 2.0 40.0 64-65 26.796723662598296 33.0 18.0 38.0 2.0 40.0 66-67 26.405820008402998 33.0 13.5 37.5 2.0 40.0 68-69 26.218010303705277 33.0 13.0 37.0 2.0 40.0 70-71 26.277838727562305 33.0 17.5 37.0 2.0 39.5 72-73 26.043613204745824 33.0 13.0 36.0 2.0 39.0 74-75 25.67669027211445 33.0 12.0 36.0 2.0 39.0 76-77 25.587598107041973 33.0 12.0 35.5 2.0 38.5 78-79 25.195097953033212 32.0 8.0 35.0 2.0 37.5 80-81 24.957806098470087 32.0 7.0 35.0 2.0 37.0 82-83 24.822817938109935 32.0 7.0 35.0 2.0 36.5 84-85 24.40346004043682 32.0 7.0 35.0 2.0 36.0 86-87 24.26901316030824 32.0 4.0 35.0 2.0 36.0 88-89 24.214155931621494 32.0 2.0 35.0 2.0 35.5 90-91 24.03958544302264 32.0 2.0 35.0 2.0 35.0 92-93 24.04979328217243 32.0 2.0 35.0 2.0 35.0 94-95 24.058080683978865 32.0 2.0 35.0 2.0 35.0 96-97 23.934988229577574 32.0 2.0 35.0 2.0 35.0 98-99 23.797204723673673 32.0 2.0 35.0 2.0 35.0 100-101 23.696371207490984 32.0 2.0 35.0 2.0 35.0 102-103 22.59515215027379 29.5 2.0 34.0 2.0 35.0 104-105 23.480108989750974 31.5 2.0 34.5 2.0 35.0 106-107 23.562700254650892 32.0 2.0 35.0 2.0 35.0 108-109 23.64031720959658 32.0 2.0 35.0 2.0 35.0 110-111 23.576718858909285 32.0 2.0 35.0 2.0 35.0 112-113 23.504763542907533 32.0 2.0 35.0 2.0 35.0 114-115 23.433029030478508 32.0 2.0 35.0 2.0 35.0 116-117 23.34449579607994 32.0 2.0 35.0 2.0 35.0 118-119 23.226445484705373 32.0 2.0 35.0 2.0 35.0 120-121 23.06632032576487 32.0 2.0 35.0 2.0 35.0 122-123 22.782343743402713 32.0 2.0 35.0 2.0 35.0 124-125 22.034947529734076 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9094675698085943 1101 2 0.8636758527107844 1101 3 0.9773932996451755 1101 4 0.8128623329774669 1101 5 1.0984039273346013 1101 6 1.0501292330536103 1101 7 1.2437148035015326 1101 8 1.1897025058354629 1101 9 1.944036063108765 1101 10-11 1.591149965999044 1101 12-13 1.7204530900379709 1101 14-15 1.8082349119866805 1101 16-17 1.7454190772192746 1101 18-19 1.7286973546776991 1101 20-21 1.8420414316245655 1101 22-23 1.9290485111673732 1101 24-25 1.9640823806249514 1101 26-27 1.8255601149951488 1101 28-29 1.8715664880512648 1101 30-31 2.294906943050645 1101 32-33 2.176116123180993 1101 34-35 2.301633167270932 1101 36-37 2.0995306190498617 1101 38-39 2.2007418449979674 1101 40-41 2.48078876370327 1101 42-43 2.4169932745389566 1101 44-45 2.1950967545150704 1101 46-47 2.140152430224852 1101 48-49 2.079817467192779 1101 50-51 2.1702498406714916 1101 52-53 2.054570071687852 1101 54-55 2.362836201016435 1101 56-57 2.1681295991300473 1101 58-59 2.1769911691825072 1101 60-61 2.2919583125360923 1101 62-63 2.2268881622729495 1101 64-65 2.521509259233831 1101 66-67 2.7678239932243436 1101 68-69 2.6417296584654153 1101 70-71 2.191750555384811 1101 72-73 2.4291281999169563 1101 74-75 2.3587008606206155 1101 76-77 2.243840462467624 1101 78-79 2.288545527050971 1101 80-81 2.333058314693801 1101 82-83 2.173377401804146 1101 84-85 2.409745050638257 1101 86-87 2.306940080998398 1101 88-89 2.182912881909303 1101 90-91 2.255732814282794 1101 92-93 2.0945231565614364 1101 94-95 2.1011121533297157 1101 96-97 2.049562889392046 1101 98-99 2.1546504421532546 1101 100-101 2.0775538510231684 1101 102-103 1.9399690701947492 1101 104-105 2.0344421643887003 1101 106-107 2.134506812105016 1101 108-109 2.0229021122674737 1101 110-111 2.0140161337859226 1101 112-113 1.8747832499551862 1101 114-115 1.9452844186235367 1101 116-117 2.108765611501884 1101 118-119 1.9967618150299629 1101 120-121 1.6527872988569037 1101 122-123 1.902534860018548 1101 124-125 1.8023705333822733 1102 1 0.6654555539017792 1102 2 -1.520174424561926 1102 3 0.05137246331644718 1102 4 0.6700279840195478 1102 5 1.0991245977355746 1102 6 0.713894284247047 1102 7 1.5140009159620078 1102 8 1.2604633061780106 1102 9 2.0747913923519867 1102 10-11 1.678459165354731 1102 12-13 1.8805976304074505 1102 14-15 1.851640215718497 1102 16-17 1.7720590313519793 1102 18-19 2.0831891883618 1102 20-21 2.079809905509336 1102 22-23 1.9722510647152163 1102 24-25 2.2592418777650423 1102 26-27 1.8734815410152947 1102 28-29 1.8493415769566326 1102 30-31 2.2090833621392214 1102 32-33 1.9401229953866412 1102 34-35 2.249097991198763 1102 36-37 1.6783517253440117 1102 38-39 1.6127613808065497 1102 40-41 1.8200658527944178 1102 42-43 1.7807622111201518 1102 44-45 1.6879857059909185 1102 46-47 1.4684268703493508 1102 48-49 1.387550236927936 1102 50-51 1.6257958766983087 1102 52-53 1.379196980779053 1102 54-55 1.743715142786698 1102 56-57 1.3583468435854336 1102 58-59 1.4123177656084636 1102 60-61 1.2171452697035363 1102 62-63 1.5673533516639075 1102 64-65 1.6394922144124315 1102 66-67 1.6993795348424676 1102 68-69 1.7254019770893052 1102 70-71 1.1517075912796848 1102 72-73 1.4212186746225903 1102 74-75 1.5888288279376006 1102 76-77 1.4376014961306645 1102 78-79 1.3459614996236837 1102 80-81 1.4672451119094987 1102 82-83 1.0495223831749918 1102 84-85 1.1710731747907914 1102 86-87 1.272037859641351 1102 88-89 1.2968013878475375 1102 90-91 1.0904431921535185 1102 92-93 1.127003420468018 1102 94-95 1.1987359724528837 1102 96-97 1.1496131035913066 1102 98-99 1.0996582020812617 1102 100-101 0.9647692291118304 1102 102-103 0.6483655903862413 1102 104-105 1.0412148686126699 1102 106-107 1.328790795294104 1102 108-109 1.2329779286081504 1102 110-111 1.2524837802408832 1102 112-113 1.122947305621551 1102 114-115 1.3760426668382806 1102 116-117 0.9938490556279369 1102 118-119 0.5703571733918977 1102 120-121 0.929136489897477 1102 122-123 1.0931713121743343 1102 124-125 1.2536867232803068 1103 1 1.0023250780782966 1103 2 0.7998857964275956 1103 3 0.7913211440406513 1103 4 0.8751354169505134 1103 5 1.1802150277958887 1103 6 1.014938293961265 1103 7 1.3648559882959006 1103 8 1.38963493898531 1103 9 2.033165425634838 1103 10-11 1.7379264963640608 1103 12-13 1.7404523950646578 1103 14-15 1.6519753742018537 1103 16-17 1.5604133195583998 1103 18-19 1.6679908563876182 1103 20-21 1.7629617693429864 1103 22-23 1.7297937735246265 1103 24-25 2.043458661378729 1103 26-27 1.5267415696322146 1103 28-29 1.6052467288981305 1103 30-31 2.2781364647449145 1103 32-33 2.1093275466118193 1103 34-35 2.125194500113917 1103 36-37 1.9914853376773713 1103 38-39 1.9994604879467381 1103 40-41 2.3886153215293966 1103 42-43 2.2581945745251524 1103 44-45 2.1687212623629577 1103 46-47 1.9131152655615402 1103 48-49 2.0415857302710663 1103 50-51 2.1145626906315833 1103 52-53 2.131134895806518 1103 54-55 2.381612546055244 1103 56-57 2.0653585860809613 1103 58-59 2.242199743222745 1103 60-61 2.2883258221474776 1103 62-63 2.27800673147733 1103 64-65 2.5203771283973815 1103 66-67 2.6924956424635944 1103 68-69 2.828793888478419 1103 70-71 2.5605991486912565 1103 72-73 2.573658132514197 1103 74-75 2.5904338248458956 1103 76-77 2.5579401197106506 1103 78-79 2.5621210971945345 1103 80-81 2.511631804743619 1103 82-83 2.4158314877163605 1103 84-85 2.5493667341918105 1103 86-87 2.499192865959177 1103 88-89 2.4082096210365904 1103 90-91 2.4636493325436994 1103 92-93 2.230128280445257 1103 94-95 2.204243683786437 1103 96-97 2.1022267124595615 1103 98-99 2.3660273483830174 1103 100-101 2.3143311632331702 1103 102-103 2.1404000501746907 1103 104-105 2.0748289942582687 1103 106-107 2.1295683510268972 1103 108-109 2.113051830755019 1103 110-111 2.127236070639878 1103 112-113 2.06486810426658 1103 114-115 1.933507709688982 1103 116-117 1.9834364402865212 1103 118-119 2.003198522748317 1103 120-121 1.982485535651687 1103 122-123 2.15197101465397 1103 124-125 1.836325095640781 1104 1 0.5448195593144973 1104 2 0.5828121809174718 1104 3 0.5374109799174143 1104 4 0.5956364247247734 1104 5 0.9851444835977858 1104 6 1.0303505216222746 1104 7 1.2914420330218874 1104 8 1.1792279901418468 1104 9 1.7831385516546128 1104 10-11 1.1503528328646908 1104 12-13 1.407639265205745 1104 14-15 1.363433767521748 1104 16-17 1.3166936727592855 1104 18-19 1.2109997823881926 1104 20-21 1.642723262768456 1104 22-23 1.4848302453549351 1104 24-25 1.6941271516369056 1104 26-27 1.3714536341297645 1104 28-29 1.3929456443410757 1104 30-31 1.954646206562746 1104 32-33 1.8853866693683266 1104 34-35 1.8727643244732626 1104 36-37 1.8268802892082228 1104 38-39 1.9485131801436886 1104 40-41 2.0150881116244115 1104 42-43 1.8789859172643766 1104 44-45 1.5692889751952848 1104 46-47 1.724877910729024 1104 48-49 1.3429841370279547 1104 50-51 1.8068349816383993 1104 52-53 1.5241658968622822 1104 54-55 1.851998859521025 1104 56-57 1.8107895295496235 1104 58-59 1.8915945987751002 1104 60-61 1.9803072982968182 1104 62-63 2.133030726102536 1104 64-65 2.1149447452512042 1104 66-67 2.10282484872139 1104 68-69 2.2067077957631867 1104 70-71 1.8760243334498696 1104 72-73 2.07755341996981 1104 74-75 2.0899088224464357 1104 76-77 1.9394498615284803 1104 78-79 1.9531120752154578 1104 80-81 1.9115247887152123 1104 82-83 1.9573781812570061 1104 84-85 2.0611845669972624 1104 86-87 1.9678712420029463 1104 88-89 1.7930440581226819 1104 90-91 1.8230825794963827 1104 92-93 1.5789323883250894 1104 94-95 1.5062760285412153 1104 96-97 1.557227192352066 1104 98-99 1.5062711337750905 1104 100-101 1.5230200969120276 1104 102-103 1.4389771689000952 1104 104-105 1.4771459152679611 1104 106-107 1.511876633971518 1104 108-109 1.3260847513807974 1104 110-111 1.5376473385158747 1104 112-113 1.5680233974809 1104 114-115 1.485319303891881 1104 116-117 1.5235544938425214 1104 118-119 1.413935157742074 1104 120-121 1.355475745844604 1104 122-123 1.4915141569900854 1104 124-125 1.30764144077947 1105 1 0.5050974596232756 1105 2 0.5713453889495383 1105 3 0.484610113037224 1105 4 0.7118271383666297 1105 5 0.8864306115968645 1105 6 0.9126999203679773 1105 7 1.2553380073990539 1105 8 1.1106019568858656 1105 9 1.5246504137209307 1105 10-11 1.1794119226404902 1105 12-13 1.4724274357433274 1105 14-15 1.405087840132559 1105 16-17 1.3505688381761374 1105 18-19 1.5564632372509735 1105 20-21 1.8011923178306972 1105 22-23 1.7290396966504602 1105 24-25 1.8626416603728373 1105 26-27 1.6592915160269257 1105 28-29 1.6957176471795279 1105 30-31 2.108282596351966 1105 32-33 1.9203411198008062 1105 34-35 2.141576391752377 1105 36-37 1.939217605331816 1105 38-39 2.0215493704737533 1105 40-41 2.0341541057557144 1105 42-43 2.103611931341586 1105 44-45 1.9186438974253903 1105 46-47 1.7089701797687447 1105 48-49 1.9285783910698768 1105 50-51 1.9420622500068596 1105 52-53 1.940630675059861 1105 54-55 2.0516834647261426 1105 56-57 1.881937858120228 1105 58-59 1.8579060427443395 1105 60-61 1.7443673470108827 1105 62-63 1.5942473310775078 1105 64-65 1.985031625057882 1105 66-67 2.123392540173416 1105 68-69 2.118571492278406 1105 70-71 2.2127001739592593 1105 72-73 2.3609404858105094 1105 74-75 2.3952430124312833 1105 76-77 2.114729392032306 1105 78-79 2.2235812837078157 1105 80-81 2.2772251972229114 1105 82-83 2.22970274350169 1105 84-85 2.4215119485204397 1105 86-87 2.3236231454953966 1105 88-89 2.2550334714442535 1105 90-91 2.188816550843306 1105 92-93 1.979331114855075 1105 94-95 1.9175066340148312 1105 96-97 1.9837504657837215 1105 98-99 1.9940331636556081 1105 100-101 2.017683221900043 1105 102-103 1.6660091590805663 1105 104-105 1.2988589625819742 1105 106-107 1.637780816860225 1105 108-109 1.7513334040725361 1105 110-111 1.8021795684309119 1105 112-113 1.8797526508515645 1105 114-115 1.7430651277157416 1105 116-117 1.7504544382241036 1105 118-119 1.8148550672183035 1105 120-121 1.9015006590606909 1105 122-123 1.9769114451126768 1105 124-125 1.8785653777964058 1106 1 0.31405533873265057 1106 2 0.29449240897539397 1106 3 0.3315016744746089 1106 4 0.21157593651468432 1106 5 0.46889233611529946 1106 6 0.33594851290018823 1106 7 0.5818477420214876 1106 8 0.25729119692317326 1106 9 1.1172873934299865 1106 10-11 0.6439628689235164 1106 12-13 0.5868965666462138 1106 14-15 0.7207175561528345 1106 16-17 0.7763788659855138 1106 18-19 0.7269013474545574 1106 20-21 0.5481134910151724 1106 22-23 0.532668131665794 1106 24-25 0.40783960609140024 1106 26-27 0.31433333735240865 1106 28-29 0.4113057228775787 1106 30-31 0.7266316131212989 1106 32-33 0.48917454529575366 1106 34-35 0.6400087504582714 1106 36-37 0.7620767248592664 1106 38-39 0.9128058796471699 1106 40-41 1.0146648451855498 1106 42-43 1.159046424353427 1106 44-45 0.9461496861771792 1106 46-47 0.9056722432631474 1106 48-49 0.8135939276602073 1106 50-51 1.2241538851479952 1106 52-53 0.876428729840061 1106 54-55 1.366399641110256 1106 56-57 1.155466699214898 1106 58-59 1.2523245540319472 1106 60-61 1.4440256052310616 1106 62-63 1.3986890356798334 1106 64-65 1.6441561708020025 1106 66-67 1.6614587666734018 1106 68-69 1.4764668057501638 1106 70-71 1.2661777250562807 1106 72-73 1.2076628235832416 1106 74-75 1.4936236367717939 1106 76-77 1.091845602433537 1106 78-79 1.048909900358467 1106 80-81 1.2026453497981073 1106 82-83 1.0034895860409385 1106 84-85 1.27794285958349 1106 86-87 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1.0955202091689387 2207 58-59 1.1085055098045977 2207 60-61 1.0036285872436252 2207 62-63 0.8148371866324879 2207 64-65 0.6024797924415104 2207 66-67 1.145464346411238 2207 68-69 1.263324507843116 2207 70-71 0.9562121074102983 2207 72-73 0.7186506438374387 2207 74-75 0.7540003597840332 2207 76-77 0.5462006414518719 2207 78-79 0.6358256162650946 2207 80-81 0.9938493553147438 2207 82-83 0.671642881332037 2207 84-85 0.6227882474458646 2207 86-87 0.9523134486925144 2207 88-89 0.9097470553978866 2207 90-91 0.8373761071194927 2207 92-93 0.7006328187638218 2207 94-95 0.3219939529577509 2207 96-97 0.5026345295554009 2207 98-99 0.43904766104279247 2207 100-101 0.36725024341915713 2207 102-103 0.4644194545692528 2207 104-105 0.1356409592990815 2207 106-107 0.04012407700749421 2207 108-109 0.344543069905459 2207 110-111 0.39412860060962274 2207 112-113 0.3768286338273015 2207 114-115 0.29390734638289473 2207 116-117 -0.05874736612667775 2207 118-119 0.015891679481207177 2207 120-121 -0.10156508660020691 2207 122-123 -2.1670156380578938 2207 124-125 -0.7593377875899918 2208 1 -0.2783533866682504 2208 2 0.04779057007060317 2208 3 0.042220429482238586 2208 4 0.26633645827585894 2208 5 0.3024517128701234 2208 6 0.2527985667780861 2208 7 0.4645360352717809 2208 8 0.21287637475571586 2208 9 0.6658978324772029 2208 10-11 0.28478473402713433 2208 12-13 0.32782873016459746 2208 14-15 0.10885927424183706 2208 16-17 0.42842994148374913 2208 18-19 0.6449888092093303 2208 20-21 0.2635390475678463 2208 22-23 0.39910573924665727 2208 24-25 0.6218864324594975 2208 26-27 0.43041266233241515 2208 28-29 0.417990839628132 2208 30-31 0.7369745532836198 2208 32-33 0.732976714563609 2208 34-35 0.776683288731487 2208 36-37 0.3345263844538344 2208 38-39 0.38105812873157774 2208 40-41 0.5681300110106235 2208 42-43 0.6216101056241179 2208 44-45 0.8746617869736895 2208 46-47 0.694188435902209 2208 48-49 0.9064594375075359 2208 50-51 0.7753949492714654 2208 52-53 0.33134602672472013 2208 54-55 1.027289372457929 2208 56-57 0.8725571784078987 2208 58-59 0.8806511224878228 2208 60-61 0.8837728294894589 2208 62-63 0.8731312806822551 2208 64-65 0.9023453254876408 2208 66-67 1.1631532546500658 2208 68-69 1.3348308098349086 2208 70-71 0.9538339409021575 2208 72-73 1.050352666057158 2208 74-75 1.194348815341499 2208 76-77 1.027082338980179 2208 78-79 1.117074557206415 2208 80-81 0.9580199830154719 2208 82-83 0.9966620629203504 2208 84-85 1.0867025365644523 2208 86-87 0.9923899812715327 2208 88-89 0.6402841852220931 2208 90-91 0.7584397998875474 2208 92-93 0.6660213381760833 2208 94-95 0.6520510586385129 2208 96-97 0.7548164481100237 2208 98-99 0.7433016652637718 2208 100-101 0.7600993761557469 2208 102-103 0.6709350721545526 2208 104-105 0.6755307264937613 2208 106-107 0.7554660081450777 2208 108-109 0.630067660062636 2208 110-111 0.7527448340259468 2208 112-113 0.5352987532210918 2208 114-115 0.44882016240497435 2208 116-117 0.490143729864549 2208 118-119 0.40524710390245033 2208 120-121 0.6340398580236482 2208 122-123 0.6150492573473088 2208 124-125 0.485430720220549 2209 1 0.3442428068743908 2209 2 0.43655663522093135 2209 3 0.3335689213548605 2209 4 0.4346781283627479 2209 5 0.7159519370139336 2209 6 0.48799027317506827 2209 7 0.7781323613271205 2209 8 0.5746938286511636 2209 9 1.0151616217522168 2209 10-11 0.6374779437786202 2209 12-13 0.7784378455074332 2209 14-15 0.3910247169449974 2209 16-17 0.17174691144606413 2209 18-19 0.7121710334973343 2209 20-21 0.35682707820767234 2209 22-23 0.8317912036512993 2209 24-25 0.5916027505721289 2209 26-27 0.27254946376596934 2209 28-29 0.5258160968699102 2209 30-31 0.7836036156133197 2209 32-33 0.9477824102733798 2209 34-35 1.146819337782933 2209 36-37 0.9822573965729084 2209 38-39 0.5367651765944395 2209 40-41 0.7439168605152737 2209 42-43 1.1170737610975614 2209 44-45 0.8519908385493231 2209 46-47 0.26865342093310574 2209 48-49 -0.13497504664551485 2209 50-51 0.2623318760483322 2209 52-53 0.687724015762015 2209 54-55 1.0372118142681401 2209 56-57 0.9658605144653265 2209 58-59 0.542583951037745 2209 60-61 0.44897189457011066 2209 62-63 0.7400537962001401 2209 64-65 1.4473759786141152 2209 66-67 1.2250431273371838 2209 68-69 1.0738728356922032 2209 70-71 1.0525150166796813 2209 72-73 1.1035028432173668 2209 74-75 1.4044252377081925 2209 76-77 1.3387400123714244 2209 78-79 1.5559470352864402 2209 80-81 1.3299320646690695 2209 82-83 1.2272761404406793 2209 84-85 1.489543040821573 2209 86-87 1.0099919163596418 2209 88-89 1.1282082107402545 2209 90-91 1.2106557826907043 2209 92-93 0.9597487148557029 2209 94-95 1.1389956381241753 2209 96-97 1.127058603177268 2209 98-99 1.1740173627014414 2209 100-101 1.0936145955810588 2209 102-103 1.0354501059009849 2209 104-105 0.892123732481803 2209 106-107 0.8450088433770233 2209 108-109 1.029128229641664 2209 110-111 0.8245373118965311 2209 112-113 0.9359249059103547 2209 114-115 0.9197691707951634 2209 116-117 0.902330004046604 2209 118-119 0.9074178551688732 2209 120-121 0.987370155782493 2209 122-123 0.9639631365819241 2209 124-125 1.1305030201938457 2210 1 0.16754683204177212 2210 2 0.1579835228722679 2210 3 0.15334367111815084 2210 4 0.24578416638792078 2210 5 -0.06846309919510674 2210 6 0.00770652146877282 2210 7 0.27874008743686574 2210 8 0.14105423059620037 2210 9 0.38426791195239574 2210 10-11 0.3891505610967769 2210 12-13 0.19254582340061432 2210 14-15 0.3985124351605691 2210 16-17 -0.007044191318385629 2210 18-19 -0.21679138381157514 2210 20-21 0.4159116046081266 2210 22-23 0.19543598617384106 2210 24-25 -0.22814595938039872 2210 26-27 -0.4761289668909967 2210 28-29 -0.21158964097302402 2210 30-31 0.038714858468036795 2210 32-33 -0.5522859197563541 2210 34-35 0.24658411801867786 2210 36-37 0.4748280231964799 2210 38-39 0.2437401650625226 2210 40-41 0.44266131090297023 2210 42-43 0.7075681729150141 2210 44-45 0.2953926246062366 2210 46-47 0.1797090631139966 2210 48-49 0.3740685992099273 2210 50-51 0.18681368381422914 2210 52-53 0.36141444822649405 2210 54-55 -0.11021208487438727 2210 56-57 0.07698374268136376 2210 58-59 0.10138027742739197 2210 60-61 0.485004140078928 2210 62-63 0.6259585053726546 2210 64-65 0.5648104346483436 2210 66-67 0.8786923033435947 2210 68-69 0.8684950861427083 2210 70-71 0.825672631542659 2210 72-73 0.6296530784371335 2210 74-75 0.9947812273737142 2210 76-77 1.0419960068141805 2210 78-79 0.832096878930102 2210 80-81 0.781512700760949 2210 82-83 0.36961483653924887 2210 84-85 0.9081825331509918 2210 86-87 0.7921062471834937 2210 88-89 0.6438119310264767 2210 90-91 0.4757751233392007 2210 92-93 0.38600710339474276 2210 94-95 0.398521509948786 2210 96-97 0.5112987223417065 2210 98-99 0.6645024550076783 2210 100-101 0.4601826894904093 2210 102-103 0.30799842834980495 2210 104-105 0.35856154902213433 2210 106-107 0.261213907082535 2210 108-109 0.30336169984389727 2210 110-111 0.5333695458146934 2210 112-113 0.5952836530547856 2210 114-115 0.46732647991552057 2210 116-117 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98-99 -3.7103562398016763 2216 100-101 -4.053071245815097 2216 102-103 -3.9276186788254748 2216 104-105 -3.843654201697518 2216 106-107 -3.9233082263095653 2216 108-109 -3.8497028466234795 2216 110-111 -3.5192440297974485 2216 112-113 -3.587689178135456 2216 114-115 -3.394907590857464 2216 116-117 -3.4130332211272325 2216 118-119 -3.553360106983696 2216 120-121 -3.4241074732646304 2216 122-123 -3.534881661494115 2216 124-125 -3.525487842624223 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84995.0 3 27283.0 4 12512.0 5 10511.0 6 11756.0 7 13674.0 8 15303.0 9 15960.0 10 17233.0 11 18607.0 12 21434.0 13 24364.0 14 26214.0 15 20857.0 16 13320.0 17 11014.0 18 9649.0 19 8779.0 20 8508.0 21 8921.0 22 10533.0 23 14137.0 24 19023.0 25 25496.0 26 30598.0 27 33501.0 28 37565.0 29 42296.0 30 46838.0 31 53340.0 32 61804.0 33 72774.0 34 89263.0 35 112597.0 36 151404.0 37 193299.0 38 68987.0 39 374.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.51054225528256 20.39483484573754 14.279419131468654 21.815203767511242 2 15.630918101777509 21.839595215681932 38.34306993742732 24.18641674511324 3 19.71374443405414 26.920246995444803 29.9988302255865 23.36717834491456 4 12.108516069173689 18.002800065737055 37.05219231001263 32.83649155507663 5 11.94983398201593 40.536282733783565 33.26194709989389 14.25193618430661 6 29.38267708251138 38.5169603563869 17.040233345930915 15.060129215170809 7 26.35057982645715 34.86153341194996 20.572396424443905 18.21549033714899 8 26.228072786271138 35.11150580422683 18.888395907035466 19.772025502466562 9 25.882677855893483 16.744732381224637 20.562972971289305 36.809616791592575 10-11 24.73499089266426 27.893123520252615 25.98696835949248 21.38491722759064 12-13 26.52878009073076 25.561322171723955 27.04749867447813 20.86239906306715 14-15 23.10371034860831 26.149153189969514 27.017282289391133 23.729854172031043 16-17 20.828324954790478 29.760930321095756 27.522725896186827 21.88801882792694 18-19 20.4738353983526 27.319685747906142 31.490759327195956 20.715719526545303 20-21 21.75127995428747 25.94192972079294 31.54060707540525 20.766183249514338 22-23 21.095605382159192 25.837700448328683 31.563633670404702 21.503060499107427 24-25 21.28287713571161 26.16406363024077 31.606178343816776 20.946880890230844 26-27 21.04695552256059 26.051679801199583 32.133435779229096 20.767928897010727 28-29 20.432041958240422 26.40719414650151 32.32008317444518 20.84068072081289 30-31 20.526436920840652 26.02573176324009 32.115490106162994 21.332341209756265 32-33 20.597831071364915 26.184924786593967 31.832929853310933 21.384314288730188 34-35 21.2717327954615 26.01255895663201 31.669488463396707 21.04621978450979 36-37 21.36378565473333 25.611049718403013 31.87820501949704 21.146959607366618 38-39 21.076611863565024 26.171829440703803 31.817085347135336 20.934473348595834 40-41 20.91252792100234 26.17147077492159 31.821671058578904 21.094330245497165 42-43 20.942653176988177 26.467881605263894 30.944248653449012 21.645216564298913 44-45 21.249808352475014 26.426627636905554 30.52606769734677 21.797496313272667 46-47 21.64418653988782 26.832491128906945 29.771433297997778 21.751889033207462 48-49 21.87080910001748 26.990961932135754 29.222740007164006 21.91548896068276 50-51 21.515267866694874 27.306556300355812 29.098081549800042 22.080094283149275 52-53 21.912381759258874 27.698027717443217 28.632740416495356 21.756850106802556 54-55 21.28452996070948 27.186529282127548 28.949352072083563 22.57958868507941 56-57 21.736325613831077 27.561962805877272 28.5030118344936 22.198699745798052 58-59 22.065787005536702 26.961742839284764 28.611436240718806 22.36103391445973 60-61 21.722399380365648 27.17410188665924 29.265160172572873 21.83833856040224 62-63 21.6452033792132 27.12818791249485 29.11605403141796 22.110554676873985 64-65 21.35992295579897 27.17504942526785 28.949718685469684 22.515308933463494 66-67 21.395557082220364 27.327746386502128 28.505479026095582 22.771217505181927 68-69 21.331628247435884 27.200400083754555 28.7670948416537 22.70087682715586 70-71 21.446966448383716 27.307484783009716 28.735928155939934 22.50962061266664 72-73 21.614109920145793 26.966388641356353 28.793092162567746 22.626409275930108 74-75 21.155293132270334 27.69934690289377 28.420557157245156 22.72480280759074 76-77 21.573776755499228 27.150078597469513 28.56843240444576 22.7077122425855 78-79 21.617703298776046 27.235711158525717 28.558094140489132 22.588491402209105 80-81 21.346966420148394 27.52411279551996 28.456749938828995 22.67217084550264 82-83 21.4359332160787 27.453885496072246 28.45487600010798 22.655305287741076 84-85 21.11183251045356 27.25055252806247 28.333147599920537 23.30446736156343 86-87 21.362505758034168 27.200590010316873 28.502329689375905 22.934574542273054 88-89 21.459017584904878 27.028424488044273 28.22143621016055 23.291121716890302 90-91 21.156971593111468 27.147786422282515 28.61488731380475 23.080354670801263 92-93 21.38635778421975 27.36932854892254 28.525630376578537 22.718683290279174 94-95 21.104517511648012 27.02342477539807 28.51272992322798 23.35932778972594 96-97 21.00928226816826 26.997798835736386 28.62261107919346 23.370307816901896 98-99 20.930344432808226 27.02850304175956 28.131897948603296 23.909254576828914 100-101 20.865095829119674 27.014404996999392 28.488198645123198 23.632300528757735 102-103 21.37275178860168 26.592056227434824 28.486008206525348 23.549183777438145 104-105 21.325432384522685 26.49532331848156 28.619470472153445 23.559773824842306 106-107 20.701187045392487 26.935606620376312 28.76696304443452 23.596243289796686 108-109 20.799938811799908 27.069445146232184 28.233889818325036 23.896726223642872 110-111 20.424005595542116 27.199013789162823 28.345324687145585 24.031655928149473 112-113 20.916551621266276 26.80260770343309 28.189243655948438 24.0915970193522 114-115 20.430976095376234 26.945904069333825 28.282563997672906 24.340555837617035 116-117 20.528295043409706 26.89633237651785 28.289056102796177 24.28631647727627 118-119 20.446026921647306 26.829795024748893 28.412118409841135 24.312059643762662 120-121 19.984619575017124 27.382901632768476 28.210343578066905 24.4221352141475 122-123 20.19380319828229 26.93545361604692 27.9726492389838 24.898093946686988 124-125 19.987020651009534 26.840428422351476 28.21371276873742 24.95883815790157 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 913.0 1 691.5 2 795.5 3 1180.0 4 876.0 5 564.5 6 984.0 7 2212.0 8 3421.0 9 3852.5 10 3121.0 11 2014.0 12 1683.0 13 1714.5 14 1933.0 15 2195.0 16 2465.5 17 2835.0 18 3264.5 19 3607.0 20 4035.5 21 4675.5 22 5362.0 23 6083.0 24 6911.5 25 8225.5 26 10348.0 27 12514.5 28 14019.5 29 15889.0 30 18519.0 31 21293.0 32 24765.0 33 28490.0 34 31881.5 35 34714.5 36 38306.0 37 42030.5 38 43048.0 39 43122.5 40 43142.0 41 43569.5 42 44638.5 43 45998.0 44 47253.5 45 47883.5 46 48245.0 47 51082.5 48 61417.5 49 63556.5 50 55380.0 51 49866.5 52 45398.0 53 43028.0 54 41139.5 55 37679.0 56 33755.0 57 30219.5 58 27333.0 59 24849.5 60 21761.0 61 17968.5 62 14577.0 63 11418.5 64 8563.5 65 6733.5 66 5289.5 67 4278.5 68 3260.5 69 2289.0 70 1768.5 71 1467.0 72 1338.5 73 1245.5 74 1026.5 75 780.0 76 528.0 77 326.0 78 207.0 79 124.5 80 82.0 81 57.5 82 37.5 83 18.5 84 12.0 85 11.5 86 7.5 87 3.5 88 2.5 89 1.5 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09939621643733781 2 2.076522627520985E-4 3 0.0 4 0.18093433827799515 5 0.0 6 6.921742091736616E-5 7 0.04042297381574184 8 0.0 9 0.0 10-11 5.883480777976124E-4 12-13 6.229567882562954E-4 14-15 4.845219464215632E-4 16-17 0.004187653965500653 18-19 0.001592000681099422 20-21 0.002941740388988062 22-23 0.001661218102016788 24-25 0.004429914938711434 26-27 0.004014610413207238 28-29 0.003357044914492259 30-31 7.267829196323448E-4 32-33 3.460871045868308E-5 34-35 0.0011766961555952247 36-37 0.0011420874451365417 38-39 3.8069581504551395E-4 40-41 0.0015227832601820558 42-43 9.344351823844432E-4 44-45 9.344351823844432E-4 46-47 1.7304355229341543E-4 48-49 3.460871045868308E-5 50-51 0.03893479926601847 52-53 6.921742091736616E-5 54-55 0.13902318991252993 56-57 3.8069581504551395E-4 58-59 0.0 60-61 0.0 62-63 5.537393673389293E-4 64-65 0.06066906943407145 66-67 0.05274367473903302 68-69 0.001765044233392837 70-71 0.0026648707053185975 72-73 0.07125933483442846 74-75 0.004706784622380899 76-77 0.0013843484183473235 78-79 0.0014189571288060064 80-81 1.7304355229341543E-4 82-83 2.7686968366946464E-4 84-85 0.0 86-87 1.7304355229341543E-4 88-89 0.0012459135765125909 90-91 2.076522627520985E-4 92-93 0.002145740048438351 94-95 0.0038761755713725057 96-97 0.0022841748902730835 98-99 0.0 100-101 0.0011074787346778585 102-103 0.001315130997429957 104-105 1.3843484183473232E-4 106-107 1.0382613137604925E-4 108-109 0.0 110-111 1.0382613137604925E-4 112-113 2.422609732107816E-4 114-115 1.7304355229341543E-4 116-117 0.0 118-119 8.30609051008394E-4 120-121 0.0020419139170623018 122-123 6.921742091736617E-4 124-125 0.008340699220542622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1444723.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.51610922512729 #Duplication Level Percentage of deduplicated Percentage of total 1 81.36364474342996 45.98356633274142 2 10.141614443622965 11.463291792298481 3 3.4879061019816024 5.913686466797406 4 1.5537245922489558 3.5124187504523343 5 0.8566185911131465 2.420637492981262 6 0.5303516774660202 1.7984047998839445 7 0.3517736321158616 1.3916613910625844 8 0.24729429881120557 1.1180889281892288 9 0.18819766819355335 0.9572579974387013 >10 1.1210474706916653 12.043031714468084 >50 0.09828064029507981 3.8821732178697976 >100 0.05473180776342979 5.479580407413818 >500 0.002913937950813167 1.1411199341816063 >1k 0.0016470084069813552 1.786468115569497 >5k 2.5338590876636236E-4 1.1086126586518046 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7755 0.5367810992141746 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7728 0.5349122288494057 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4496 0.31120152444447824 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3855 0.26683315763644655 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2277 0.15760806742884276 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2242 0.15518545769673495 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1713 0.11856944203144824 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1676 0.11600839745750569 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1466 0.10147273906485879 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1452 0.10050369517201568 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1447 0.10015760806742884 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0382613137604926E-4 0.0 12-13 0.0 0.0 0.0 1.3843484183473235E-4 0.0 14-15 0.0 0.0 0.0 1.7304355229341543E-4 0.0 16-17 0.0 0.0 0.0 2.422609732107816E-4 0.0 18-19 0.0 0.0 0.0 4.15304525504197E-4 0.0 20-21 0.0 0.0 0.0 7.613916300910278E-4 0.0 22-23 0.0 0.0 0.0 0.001280522286971274 0.0 24-25 0.0 0.0 0.0 0.001972696496144936 0.0 26-27 0.0 0.0 0.0 0.0026994794157772805 0.0 28-29 0.0 0.0 0.0 0.0045337410700874835 0.0 30-31 0.0 0.0 0.0 0.007579307590451595 0.0 32-33 0.0 0.0 0.0 0.013324353526592987 0.0 34-35 0.0 0.0 0.0 0.022253400824933222 0.0 36-37 0.0 0.0 0.0 0.03599305887703041 0.0 38-39 0.0 0.0 0.0 0.0628148094825098 0.0 40-41 0.0 0.0 0.0 0.09192073497826227 0.0 42-43 0.0 0.0 0.0 0.12227257405052733 0.0 44-45 0.0 0.0 0.0 0.1552546751176523 0.0 46-47 0.0 0.0 0.0 0.20211486907870918 0.0 48-49 0.0 0.0 0.0 0.2529550647425146 0.0 50-51 0.0 0.0 0.0 0.3140048299916316 0.0 52-53 0.0 0.0 0.0 0.36899807091047904 0.0 54-55 0.0 0.0 0.0 0.42897496613537683 0.0 56-57 0.0 0.0 0.0 0.5038336068575083 0.0 58-59 0.0 0.0 0.0 0.5891786868486208 0.0 60-61 0.0 0.0 0.0 0.6897515994415538 0.0 62-63 0.0 0.0 0.0 0.7874519890664162 0.0 64-65 0.0 0.0 0.0 0.8830066386428401 0.0 66-67 0.0 0.0 0.0 0.9971115570251183 0.0 68-69 0.0 0.0 0.0 1.1334352675218708 0.0 70-71 0.0 0.0 0.0 1.2929468140259415 0.0 72-73 0.0 0.0 0.0 1.4541887960529458 0.0 74-75 0.0 6.921742091736617E-5 0.0 1.6120737331654582 0.0 76-77 0.0 6.921742091736617E-5 0.0 1.7754960639513595 0.0 78-79 0.0 6.921742091736617E-5 0.0 1.9856401538564832 0.0 80-81 0.0 6.921742091736617E-5 0.0 2.2235404295494705 0.0 82-83 0.0 6.921742091736617E-5 0.0 2.469435317358414 0.0 84-85 0.0 6.921742091736617E-5 0.0 2.7055705488180086 0.0 86-87 0.0 6.921742091736617E-5 0.0 2.9544071770159404 0.0 88-89 0.0 6.921742091736617E-5 0.0 3.239202255380443 0.0 90-91 0.0 6.921742091736617E-5 0.0 3.577225530430401 0.0 92-93 0.0 6.921742091736617E-5 0.0 3.9409284686407013 0.0 94-95 0.0 6.921742091736617E-5 0.0 4.289403574249182 0.0 96-97 0.0 6.921742091736617E-5 0.0 4.62635397927492 0.0 98-99 0.0 6.921742091736617E-5 0.0 5.003588923274565 0.0 100-101 0.0 6.921742091736617E-5 0.0 5.451183375636713 0.0 102-103 0.0 6.921742091736617E-5 0.0 5.948891240743035 0.0 104-105 6.921742091736617E-5 6.921742091736617E-5 0.0 6.436216492711751 0.0 106-107 6.921742091736617E-5 6.921742091736617E-5 0.0 6.907552520448556 0.0 108-109 6.921742091736617E-5 6.921742091736617E-5 0.0 7.392489771395624 0.0 110-111 6.921742091736617E-5 6.921742091736617E-5 0.0 7.954950533770141 0.0 112-113 6.921742091736617E-5 6.921742091736617E-5 0.0 8.564444533658008 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10010 0.0 31.111696 1 TACATGG 9960 0.0 29.858038 2 ACATGGG 10045 0.0 27.70684 3 GAGTACT 9735 0.0 26.695166 12-13 AGTACTT 10015 0.0 25.11751 12-13 GTACTTT 10690 0.0 24.81143 14-15 GTATCAA 19670 0.0 24.112934 1 CATGGGG 3365 0.0 24.086512 4 ACCGCAG 420 0.0 24.079758 6 GGTATCA 15570 0.0 23.48871 1 CATGGGA 6385 0.0 22.867846 4 CACCGCA 500 0.0 22.606646 5 TCACCGC 455 0.0 22.266788 4 ACTTTTT 12295 0.0 22.225887 16-17 CCGCAGA 460 0.0 21.985867 7 TACTTTT 11695 0.0 21.662273 14-15 CGTATAC 140 0.0031265526 21.28443 3 ATGGGAT 2330 0.0 20.68148 5 GTATCAC 670 0.0 20.48049 1 TATCAAC 23015 0.0 20.40494 2 >>END_MODULE