FastQCFastQC Report
Thu 26 May 2016
SRR1364498_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364498_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2703963
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61500.050.628171
ATCAACG71450.043.14123
GGTATCA36550.042.3498271
TCAACGC73600.041.759444
CAACGCA74200.041.420235
AACGCAG74900.039.976396
TATCAAC80500.039.4731752
ACGCAGA85050.034.086327
GTACATG100450.033.7824061
CGCAGAG84750.033.365958
TACATGG102400.030.6824952
ACATGGG100100.029.7235473
GCAGAGT97450.027.5519499
CATGGGA63100.023.0326164
AGAGTAC91250.022.1081110-11
GAGTACT43600.021.96622712-13
CAGAGTA95800.021.27550510-11
CATGGGG49750.020.4732044
GAGTACA81550.020.3678841
CGCATAG3352.0507287E-819.5477818
Produced by FastQC (version 0.11.5)