FastQCFastQC Report
Thu 26 May 2016
SRR1364498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364498_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2703963
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63050.23317626757466725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57460.21250290776907818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34630.12807127908185134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54200.056.0166741
GTATCAA88050.047.2851641
ATCAACG111450.035.987143
TCAACGC112650.035.5508884
CAACGCA113200.035.2712635
TATCAAC118400.034.5800362
AACGCAG115750.033.9801566
GTACATG95400.029.0739061
ACGCAGA134350.028.8328937
CGCAGAG136500.028.7115548
TACATGG97050.027.3265572
GCAGAGT145300.025.9903459
ACATGGG96950.025.448663
GAGTACT91600.024.41340812-13
CAGAGTA139850.023.4115910-11
GTACTTT97600.023.2782114-15
AGAGTAC137500.022.96824610-11
AGTACTT98450.022.29188212-13
GAGTACA83200.020.8626041
GTGGTAT32050.020.6582621
Produced by FastQC (version 0.11.5)