##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364498_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2703963 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.90045388934686 31.0 28.0 31.0 25.0 34.0 2 29.09076270644236 31.0 28.0 33.0 25.0 34.0 3 29.208573490095834 31.0 28.0 34.0 25.0 34.0 4 32.64304282270135 35.0 32.0 37.0 26.0 37.0 5 31.753242185636417 35.0 32.0 37.0 20.0 37.0 6 31.810171588886387 35.0 32.0 37.0 21.0 37.0 7 31.269436009294505 35.0 31.0 37.0 17.0 37.0 8 31.393581199151026 35.0 32.0 37.0 17.0 37.0 9 31.98703791435016 37.0 30.0 39.0 11.0 39.0 10-11 32.1764669117144 37.0 31.0 39.0 16.5 39.0 12-13 31.91564973337283 37.0 30.5 39.0 12.5 39.0 14-15 32.861972963387444 38.0 31.0 40.0 13.5 41.0 16-17 32.91659593714854 38.0 31.0 40.0 13.5 41.0 18-19 32.77634105940059 38.0 31.0 40.0 11.0 41.0 20-21 32.653096954359214 38.0 31.0 40.0 10.5 41.0 22-23 32.62103401562817 38.0 31.0 40.0 10.0 41.0 24-25 32.425748799077496 38.0 30.5 40.0 10.0 41.0 26-27 32.508557069752804 37.0 31.0 40.0 10.0 41.0 28-29 32.39494475331209 37.0 30.5 40.0 9.5 41.0 30-31 31.884919098375235 37.0 30.0 40.0 8.0 41.0 32-33 31.82186793976101 37.0 30.0 40.0 8.0 41.0 34-35 31.629623815118773 37.0 30.0 40.0 4.5 41.0 36-37 31.614509333152856 37.0 30.0 40.0 2.0 41.0 38-39 31.38945521813723 37.0 30.0 40.0 2.0 41.0 40-41 31.106465029292192 36.0 29.5 40.0 2.0 41.0 42-43 30.993727170083318 36.0 29.0 40.0 2.0 41.0 44-45 30.923357494166893 36.0 29.0 40.0 2.0 41.0 46-47 30.787786667199217 36.0 29.0 40.0 2.0 41.0 48-49 30.51730201189883 36.0 28.0 40.0 2.0 41.0 50-51 30.179689588947777 36.0 27.5 40.0 2.0 41.0 52-53 30.09088752323904 36.0 27.5 40.0 2.0 41.0 54-55 29.624210834245883 35.0 26.0 40.0 2.0 41.0 56-57 29.646869058489337 35.0 27.0 39.0 2.0 41.0 58-59 29.403689140716793 35.0 26.0 39.0 2.0 41.0 60-61 29.12921275180171 35.0 26.0 39.0 2.0 41.0 62-63 28.861368850091512 34.5 26.0 39.0 2.0 40.5 64-65 28.38731391664753 34.0 25.0 38.5 2.0 40.0 66-67 27.998166949769654 34.0 23.0 38.0 2.0 40.0 68-69 27.80563398981421 34.0 23.0 37.5 2.0 40.0 70-71 27.876942103127888 34.0 25.0 37.0 2.0 40.0 72-73 27.650277389150666 34.0 24.5 37.0 2.0 39.0 74-75 27.279931345214415 34.0 23.5 36.0 2.0 39.0 76-77 27.189667721044998 34.0 24.0 36.0 2.0 39.0 78-79 26.792058730093572 33.0 23.0 35.5 2.0 37.5 80-81 26.561451654479 33.0 22.5 35.0 2.0 37.0 82-83 26.42418073028366 33.0 23.0 35.0 2.0 37.0 84-85 25.98948654253035 33.0 20.5 35.0 2.0 36.0 86-87 25.856929994974045 33.0 20.0 35.0 2.0 36.0 88-89 25.81392737252692 33.0 20.5 35.0 2.0 36.0 90-91 25.64906416988694 33.0 20.0 35.0 2.0 35.0 92-93 25.663034405426405 33.0 20.0 35.0 2.0 35.0 94-95 25.67790886931515 33.0 20.5 35.0 2.0 35.0 96-97 25.55891334311897 33.0 20.0 35.0 2.0 35.0 98-99 25.420090992369346 33.0 19.5 35.0 2.0 35.0 100-101 25.32862764764163 33.0 19.0 35.0 2.0 35.0 102-103 24.20472746853415 30.5 17.5 34.0 2.0 35.0 104-105 25.152284813068817 32.0 19.0 35.0 2.0 35.0 106-107 25.256981327037387 33.0 19.0 35.0 2.0 35.0 108-109 25.331654871017093 33.0 19.0 35.0 2.0 35.0 110-111 25.262569791080722 33.0 19.0 35.0 2.0 35.0 112-113 25.212955021943717 33.0 18.5 35.0 2.0 35.0 114-115 25.162359655069245 33.0 18.0 35.0 2.0 35.0 116-117 25.085384304444993 33.0 17.5 35.0 2.0 35.0 118-119 24.965411878786803 33.0 11.5 35.0 2.0 35.0 120-121 24.8039464667231 33.0 2.0 35.0 2.0 35.0 122-123 24.52139064033051 33.0 2.0 35.0 2.0 35.0 124-125 23.76098101194432 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8028669968479569 1101 2 0.8290420397476055 1101 3 0.827893136902123 1101 4 0.9410853974895517 1101 5 1.1112355762942343 1101 6 1.15674319640749 1101 7 1.5295052592504952 1101 8 1.4295950850233439 1101 9 1.982272266538299 1101 10-11 1.680523602001827 1101 12-13 1.7478051452615126 1101 14-15 1.7686009209067564 1101 16-17 1.763671747197364 1101 18-19 1.7570407753066988 1101 20-21 1.9576463775194881 1101 22-23 1.998597629639022 1101 24-25 2.1075285777327224 1101 26-27 1.8433586560978341 1101 28-29 1.9862711184400013 1101 30-31 2.3549962068202106 1101 32-33 2.3031672999945236 1101 34-35 2.333894510437556 1101 36-37 2.142854301541945 1101 38-39 2.288235202715505 1101 40-41 2.4230639003817664 1101 42-43 2.46433939053804 1101 44-45 2.3414895249253505 1101 46-47 2.22896799346886 1101 48-49 2.245439780086109 1101 50-51 2.329124273874367 1101 52-53 2.3241272697761595 1101 54-55 2.618019478755887 1101 56-57 2.3545565021441632 1101 58-59 2.3537990676295344 1101 60-61 2.4513313098834004 1101 62-63 2.563198874222117 1101 64-65 2.719748541684094 1101 66-67 2.9360952952221027 1101 68-69 2.930461589682558 1101 70-71 2.4597281321981406 1101 72-73 2.621952265970272 1101 74-75 2.734022147348746 1101 76-77 2.5402560033624972 1101 78-79 2.6072759684223925 1101 80-81 2.6568177488463363 1101 82-83 2.4926304149439176 1101 84-85 2.7132246035505077 1101 86-87 2.6244464415328856 1101 88-89 2.5009965655298423 1101 90-91 2.4770752585421363 1101 92-93 2.4318644168841743 1101 94-95 2.3572624191773777 1101 96-97 2.2174369581481166 1101 98-99 2.3748852293761686 1101 100-101 2.4472365796896476 1101 102-103 2.199905251466337 1101 104-105 2.2947371700112775 1101 106-107 2.418474993966335 1101 108-109 2.39389150205551 1101 110-111 2.4465821289044207 1101 112-113 2.300932583058735 1101 114-115 2.3022390603466967 1101 116-117 2.329189041649421 1101 118-119 2.2923722316261497 1101 120-121 2.2288245223949303 1101 122-123 2.2717558216493536 1101 124-125 2.1899966136463362 1102 1 0.5642008650498873 1102 2 -1.6520685613800161 1102 3 -0.020936741277569837 1102 4 0.5046039277724645 1102 5 1.0126241096150963 1102 6 0.5734170178534512 1102 7 1.3099781155437107 1102 8 1.0323007415713832 1102 9 1.7798743343495325 1102 10-11 1.5576242461329315 1102 12-13 1.592015417842422 1102 14-15 1.6549365894335182 1102 16-17 1.5956335028272548 1102 18-19 1.6900369836272162 1102 20-21 1.8362808425807344 1102 22-23 1.8000909390737192 1102 24-25 2.068005139624759 1102 26-27 1.7164584481310072 1102 28-29 1.6853382822233343 1102 30-31 2.066848188969466 1102 32-33 1.7972981294044494 1102 34-35 2.004369026937294 1102 36-37 1.2733187898137075 1102 38-39 1.183230880261199 1102 40-41 1.504478050231544 1102 42-43 1.387536054003018 1102 44-45 1.2811303832889678 1102 46-47 0.9754801362551042 1102 48-49 1.1079672273639503 1102 50-51 1.2921903169345654 1102 52-53 1.0786809192885833 1102 54-55 1.405721574289327 1102 56-57 0.9361620446660481 1102 58-59 0.9470891927948131 1102 60-61 1.1099802880748513 1102 62-63 1.338411711693702 1102 64-65 1.426612870433118 1102 66-67 1.4844621800974416 1102 68-69 1.3730930527510026 1102 70-71 0.8093271817159504 1102 72-73 1.127825204682079 1102 74-75 1.403836349360489 1102 76-77 1.3558489823524553 1102 78-79 1.2263932465500815 1102 80-81 1.3890432395032377 1102 82-83 0.929691477607836 1102 84-85 0.9318404479497424 1102 86-87 1.0335420398419544 1102 88-89 1.177776377820436 1102 90-91 0.8542123557620229 1102 92-93 0.9619066466431363 1102 94-95 0.9378014436882545 1102 96-97 1.021156334343658 1102 98-99 0.8047185042245317 1102 100-101 0.7682565750741155 1102 102-103 0.5123781419238576 1102 104-105 0.8593743579452031 1102 106-107 1.0845368445305539 1102 108-109 1.006429137218035 1102 110-111 0.910414440453394 1102 112-113 0.9500655466211647 1102 114-115 1.2660259869627772 1102 116-117 0.8740891707927219 1102 118-119 0.424009722237944 1102 120-121 0.7567355862367364 1102 122-123 1.147034592877695 1102 124-125 1.1653430770882416 1103 1 0.7139824050843409 1103 2 0.6545335216891175 1103 3 0.6095480925518615 1103 4 0.728603568595453 1103 5 0.9679010579337728 1103 6 0.9029599572289371 1103 7 1.1727634387518187 1103 8 0.9351031887010777 1103 9 1.7753791080168178 1103 10-11 1.4676561152160659 1103 12-13 1.4249866860444662 1103 14-15 1.3282022803884814 1103 16-17 1.290944273647895 1103 18-19 1.4089888483939248 1103 20-21 1.7066114429655954 1103 22-23 1.5899698271057972 1103 24-25 1.771410192835738 1103 26-27 1.3961857807927203 1103 28-29 1.5202277210801398 1103 30-31 2.21106662057894 1103 32-33 1.9409779924130781 1103 34-35 2.0036995083841553 1103 36-37 1.9096650616587212 1103 38-39 1.920958408264795 1103 40-41 2.1547963866118387 1103 42-43 2.084515304441549 1103 44-45 1.9938074807921353 1103 46-47 1.9050759282128418 1103 48-49 1.91815812679517 1103 50-51 2.015863075169843 1103 52-53 1.893844175480254 1103 54-55 2.1151196062198494 1103 56-57 1.8891313200658146 1103 58-59 1.961263491316597 1103 60-61 2.00834697787273 1103 62-63 2.0656782092216055 1103 64-65 2.194875761225191 1103 66-67 2.4576818318060134 1103 68-69 2.535218348816045 1103 70-71 2.202569494658377 1103 72-73 2.3048902165089267 1103 74-75 2.3094025296941325 1103 76-77 2.1655316384349526 1103 78-79 2.2666575015846675 1103 80-81 2.4006298488171076 1103 82-83 2.3031451851258993 1103 84-85 2.359557846531466 1103 86-87 2.2409431342291057 1103 88-89 2.130388051545932 1103 90-91 2.206747546838681 1103 92-93 1.9673149781320092 1103 94-95 1.8770440134107353 1103 96-97 1.7189458991487214 1103 98-99 1.9667715316692735 1103 100-101 1.8934118443032801 1103 102-103 1.7708613358266696 1103 104-105 1.7610220824879441 1103 106-107 1.8346965443972039 1103 108-109 1.8457653607710682 1103 110-111 1.8350016428819345 1103 112-113 1.7845595756971768 1103 114-115 1.6047663390035112 1103 116-117 1.678764812683788 1103 118-119 1.7774692427523888 1103 120-121 1.7686585301649522 1103 122-123 1.9261948827083408 1103 124-125 1.807959784017914 1104 1 0.6562367412248413 1104 2 0.6325989746545844 1104 3 0.6840068801523174 1104 4 0.697204639861674 1104 5 0.938814124009717 1104 6 1.0650509528769874 1104 7 1.4253153166407593 1104 8 1.2048963456025916 1104 9 1.8172361140094502 1104 10-11 1.2776783678620163 1104 12-13 1.421928203226674 1104 14-15 1.5472056687898572 1104 16-17 1.2684839511341934 1104 18-19 1.2651733102318943 1104 20-21 1.5832650549722729 1104 22-23 1.5782853634697673 1104 24-25 1.7503939812664697 1104 26-27 1.476140910599284 1104 28-29 1.5499903309657412 1104 30-31 2.1010000033132386 1104 32-33 1.960655041885044 1104 34-35 1.9162076552069855 1104 36-37 1.856573319919299 1104 38-39 1.9001520150634263 1104 40-41 2.0206625051999687 1104 42-43 2.029351027897402 1104 44-45 1.717099334519304 1104 46-47 1.8286386076095305 1104 48-49 1.625630415963009 1104 50-51 1.9711581451071787 1104 52-53 1.7610122785191322 1104 54-55 1.9922329941283472 1104 56-57 1.9550137058574535 1104 58-59 2.022710684356685 1104 60-61 2.092268164012058 1104 62-63 2.186384085278206 1104 64-65 2.26537370159415 1104 66-67 2.212795053869417 1104 68-69 2.36526393717795 1104 70-71 2.1773657209633086 1104 72-73 2.326476652791939 1104 74-75 2.4391656314034975 1104 76-77 2.2754588527472137 1104 78-79 2.324722189843488 1104 80-81 2.204341024089775 1104 82-83 2.12244508509 1104 84-85 2.3544515258661747 1104 86-87 2.286483027694789 1104 88-89 2.1338277506724737 1104 90-91 2.2116415635750712 1104 92-93 1.9979857697017493 1104 94-95 1.9254179223816656 1104 96-97 2.0297928292058813 1104 98-99 2.048608945095072 1104 100-101 1.977850238668946 1104 102-103 1.8974328714358712 1104 104-105 1.7796453598619557 1104 106-107 1.8782155445543722 1104 108-109 1.800968174087668 1104 110-111 1.8040393002946296 1104 112-113 1.837910714813919 1104 114-115 1.7534815260774081 1104 116-117 1.8392892967289214 1104 118-119 1.7949183040882097 1104 120-121 1.7889575780575875 1104 122-123 1.879839698790576 1104 124-125 1.6723886666175716 1105 1 0.4287628162888737 1105 2 0.49633889541291154 1105 3 0.44822852784434986 1105 4 0.5537005337546717 1105 5 0.6643556190688926 1105 6 0.7728665853999672 1105 7 1.1137051623811054 1105 8 0.9803588036721607 1105 9 1.5556762879974322 1105 10-11 1.293640689583718 1105 12-13 1.3711955226574197 1105 14-15 1.277208825365605 1105 16-17 1.1730329275230957 1105 18-19 1.4204001615299902 1105 20-21 1.5490702568300136 1105 22-23 1.4763439574471349 1105 24-25 1.6685190584218859 1105 26-27 1.4400787916190865 1105 28-29 1.5188991525129225 1105 30-31 1.8258513372303398 1105 32-33 1.7170466813620955 1105 34-35 1.850998514207081 1105 36-37 1.603468874040658 1105 38-39 1.6589445070397986 1105 40-41 1.7565586027939055 1105 42-43 1.8353429890232285 1105 44-45 1.69052125452329 1105 46-47 1.7087659377562296 1105 48-49 1.6878164240918565 1105 50-51 1.8004614341503817 1105 52-53 1.7164665823678504 1105 54-55 1.8431578143229252 1105 56-57 1.6673328157308092 1105 58-59 1.631139541151768 1105 60-61 1.528348704346186 1105 62-63 1.4475873173729816 1105 64-65 1.7561413038481248 1105 66-67 1.9232013491965567 1105 68-69 1.9191385086522708 1105 70-71 1.7367607553850135 1105 72-73 2.0264277903638472 1105 74-75 1.9971638997109444 1105 76-77 1.8072107959975021 1105 78-79 1.9284437670695809 1105 80-81 1.8927739382733435 1105 82-83 1.8275736571856172 1105 84-85 2.0912483029394338 1105 86-87 2.0535137453024674 1105 88-89 1.883366097471093 1105 90-91 1.79408852915806 1105 92-93 1.6227726661918638 1105 94-95 1.5136772355593955 1105 96-97 1.6026606107908812 1105 98-99 1.7395715972924464 1105 100-101 1.760694324081772 1105 102-103 1.6027676443639045 1105 104-105 1.1726913573857303 1105 106-107 1.447894848824184 1105 108-109 1.312114079312785 1105 110-111 1.4249593115350692 1105 112-113 1.4780927408174485 1105 114-115 1.383700190107092 1105 116-117 1.3069568089869001 1105 118-119 1.4285319660806692 1105 120-121 1.4484573466180066 1105 122-123 1.575051681881309 1105 124-125 1.3898730580380807 1106 1 0.3138381103613952 1106 2 0.15505183011214996 1106 3 0.34091806132649793 1106 4 0.11150910027808436 1106 5 0.1757415699783671 1106 6 0.5172518030645321 1106 7 0.7754264830874824 1106 8 0.40388191678776764 1106 9 1.1460061432942616 1106 10-11 0.7829567555157624 1106 12-13 0.6124228530049898 1106 14-15 0.8036352309688937 1106 16-17 0.7604652933436142 1106 18-19 0.8525651716336213 1106 20-21 0.5491027963146706 1106 22-23 0.4959979701065578 1106 24-25 0.38876014239818346 1106 26-27 0.427517566980427 1106 28-29 0.41668148417928563 1106 30-31 0.8400923205555344 1106 32-33 0.6186802290626012 1106 34-35 0.6591315329975309 1106 36-37 0.7039440816581504 1106 38-39 0.9112910792714715 1106 40-41 1.0812922503094917 1106 42-43 1.1679793872481632 1106 44-45 1.0130291848956823 1106 46-47 0.9149269326349874 1106 48-49 0.9839655686968385 1106 50-51 1.1838294298213974 1106 52-53 0.8948562335986701 1106 54-55 1.4094967255960285 1106 56-57 1.101363805450795 1106 58-59 1.0744029744263983 1106 60-61 1.2631179715324024 1106 62-63 1.346052896253692 1106 64-65 1.472501009245299 1106 66-67 1.4734026432025225 1106 68-69 1.4436178727995923 1106 70-71 1.2537730864563201 1106 72-73 1.2291785325017912 1106 74-75 1.4043751412914531 1106 76-77 1.0078604054171372 1106 78-79 0.9775589635662669 1106 80-81 1.144569142461478 1106 82-83 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-4.6227353969480625 2216 104-105 -4.3197052099928825 2216 106-107 -4.494449190613615 2216 108-109 -4.5282536223811505 2216 110-111 -4.233105061471672 2216 112-113 -4.379674011803019 2216 114-115 -4.1774667214715535 2216 116-117 -4.1351383758954015 2216 118-119 -4.367867386425491 2216 120-121 -4.3036910680954605 2216 122-123 -4.341581403441513 2216 124-125 -4.246799152118548 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 136080.0 3 45305.0 4 21743.0 5 17478.0 6 17373.0 7 20834.0 8 22752.0 9 24183.0 10 26013.0 11 28192.0 12 30160.0 13 32288.0 14 32777.0 15 26873.0 16 19966.0 17 17169.0 18 15399.0 19 14433.0 20 13907.0 21 15207.0 22 18226.0 23 25216.0 24 34504.0 25 46253.0 26 56413.0 27 64488.0 28 72753.0 29 82036.0 30 92390.0 31 104914.0 32 123020.0 33 146023.0 34 176225.0 35 222455.0 36 312899.0 37 402438.0 38 144866.0 39 712.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.098041654681296 20.416968846172214 14.290712047827473 24.19427745131902 2 15.787184642962412 20.80871048710428 38.48948893086027 24.91461593907304 3 18.948521115118808 25.129042076389357 29.592749604931722 26.32968720356011 4 13.04562396721773 17.518395565797956 36.63818719387037 32.797793273113946 5 13.114935374485523 38.565838363912526 34.28771029781103 14.031515963790925 6 31.499529763927807 37.710751005654295 17.011044168166627 13.778675062251267 7 27.550656108940462 32.484135108607866 21.181169974927684 18.784038807523988 8 25.5485744442509 36.647025125713625 19.196379536258448 18.608020893777024 9 25.231965082362446 16.308877007562604 20.100607885536895 38.358550024538054 10-11 24.69536656882819 27.17962430099856 27.247931865515827 20.877077264657416 12-13 25.597945668653455 24.288803158199748 27.866001737091224 22.247249436055572 14-15 22.450540422245155 25.64629049237403 25.85339487538696 26.04977420999386 16-17 21.98236295302927 28.87964734782277 26.517001287791427 22.620988411356528 18-19 21.945315710037384 27.369660578101207 28.864600703940685 21.82042300792072 20-21 22.579412762183516 26.56646247853547 29.0969381396135 21.75718661966752 22-23 22.27297783529108 26.400325749824656 28.942638924922026 22.38405748996224 24-25 22.111402568751693 26.65743289411273 28.92367066498881 22.307493872146768 26-27 22.0535059465229 26.77642131642706 28.85260974262986 22.317462994420175 28-29 21.976352822588474 26.767847398242782 29.059595776357224 22.19620400281152 30-31 22.022084419324745 26.59418608198954 28.89107294256442 22.492656556121293 32-33 21.885459189145408 26.655607586201285 28.922096538338927 22.53683668631438 34-35 22.25867324720569 26.71679119208782 28.795171477098634 22.229364083607855 36-37 22.38355934378503 26.455445793550204 28.76624810322453 22.39474675944024 38-39 22.164345955553515 26.670274970103907 28.80176896860277 22.36361010573981 40-41 22.260986914928356 26.649946503614565 28.641276871624576 22.4477897098325 42-43 22.256632913452222 26.66601329911167 28.42509449174168 22.65225929569443 44-45 22.261615565961414 26.759686981020742 28.455332811499428 22.523364641518416 46-47 22.484488014327148 26.952993898757654 27.951456337608853 22.61106174930634 48-49 22.507990127080188 27.059773769010064 27.611205334986217 22.821030768923528 50-51 22.530742642345718 27.12161923428044 27.4790894535741 22.86854866979974 52-53 22.693541068084933 27.200133433877188 27.36492871014042 22.741396787897457 54-55 22.521741835424194 27.028960228476283 27.416395217759053 23.032902718340466 56-57 22.435935959721213 27.30114356584033 27.446097928053153 22.816822546385303 58-59 22.499531243585803 27.111780005865466 27.443515314373755 22.945173436174976 60-61 22.56918826182163 27.043269453021363 27.604556719156292 22.78298556600072 62-63 22.516569762012672 27.09350943149208 27.644796226552558 22.74512457994269 64-65 22.376071285839043 26.95947246110807 27.552787193416123 23.11166905963676 66-67 22.234350653843748 27.185051046101893 27.39474135050621 23.18585694954815 68-69 22.358169106985777 27.061736951469083 27.58967504716771 22.99041889437743 70-71 22.36121553896298 26.98901618299877 27.62066240194209 23.029105876096153 72-73 22.4245703453743 27.027087541551726 27.34153351487424 23.20680859819974 74-75 22.32969384549125 27.189235800015126 27.311988303931358 23.16908205056227 76-77 22.446109975204422 27.073021489809477 27.395554473994043 23.085314060992058 78-79 22.45350136570062 27.163009604376754 27.33413130955813 23.0493577203645 80-81 22.34535230821604 27.188766256015946 27.383499130257267 23.082382305510748 82-83 22.434996690962528 27.153652691045373 27.34744264751105 23.063907970481058 84-85 22.320960013136272 27.098207334937648 27.24600891358351 23.33482373834257 86-87 22.333618532378168 27.058744777797152 27.44016691078398 23.1674697790407 88-89 22.44680512706339 26.953714709648118 27.289596682749455 23.30988348053904 90-91 22.34412618576527 27.17875700364282 27.33047948371826 23.14663732687365 92-93 22.4149526709929 27.12769724672754 27.34331240732108 23.11403767495848 94-95 22.279266532992757 27.006999480554256 27.379284340976096 23.334449645476894 96-97 22.38081642193339 26.951036578329933 27.330137697603753 23.338009302132924 98-99 22.156830548347 26.845596629835537 27.29684170974233 23.70073111207513 100-101 22.155101874769436 26.881717646427933 27.447671123447055 23.51550935535558 102-103 22.135870338047656 26.757191910200124 27.547432462493855 23.559505289258368 104-105 22.18626263724539 26.782888735656922 27.497580864224584 23.533267762873102 106-107 21.869700485861028 26.86919659573681 27.4851999611681 23.77590295723406 108-109 22.111082880941787 26.815807021028025 27.23833499200988 23.834775106020313 110-111 22.002758175876057 26.795967101596506 27.328574635506946 23.872700087020483 112-113 22.06484193553159 26.850082841462154 27.430157990502817 23.65491723250344 114-115 22.033663244769333 26.953229303117876 27.26795312649087 23.74515432562192 116-117 21.761484901975358 26.884206625608414 27.41280113670194 23.94150733571428 118-119 21.81410964304655 26.86542738775815 27.299257601320743 24.02120536787456 120-121 21.750460260083006 26.934571689992183 27.384106548082666 23.93086150184215 122-123 21.79579138783904 26.89763200614803 27.309602543385054 23.996974062627878 124-125 21.712250033656783 26.889569503847227 27.222142908922525 24.17603755357346 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 233.0 1 190.0 2 296.5 3 499.5 4 423.0 5 359.0 6 641.5 7 1392.0 8 2330.5 9 2752.0 10 2262.5 11 1584.0 12 1442.5 13 1525.0 14 1635.5 15 1810.5 16 2053.0 17 2350.0 18 2722.0 19 3100.5 20 3587.0 21 4368.5 22 5377.0 23 6671.0 24 8312.5 25 10670.0 26 14217.0 27 18380.0 28 22385.0 29 26657.0 30 32162.0 31 38359.5 32 44188.0 33 49505.0 34 54925.5 35 59928.5 36 65324.0 37 71226.0 38 75173.5 39 77309.0 40 79278.5 41 82864.0 42 86162.0 43 89028.5 44 92891.5 45 96069.0 46 97868.5 47 98895.0 48 100899.0 49 101408.5 50 99160.0 51 97175.5 52 95774.0 53 94449.0 54 92166.0 55 86802.5 56 79493.0 57 71849.5 58 65563.5 59 59081.0 60 50479.0 61 41508.0 62 32729.0 63 25125.5 64 18625.0 65 13583.0 66 10162.0 67 7524.0 68 5389.5 69 3793.0 70 2706.0 71 2007.5 72 1507.0 73 1169.5 74 869.0 75 567.5 76 406.0 77 257.0 78 145.0 79 97.0 80 69.5 81 46.5 82 35.5 83 25.0 84 17.0 85 9.0 86 3.0 87 3.5 88 3.0 89 1.5 90 1.5 91 1.0 92 1.0 93 2.0 94 1.5 95 0.5 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10185050609050493 2 1.4793101828686266E-4 3 0.0 4 0.18280575584799053 5 0.0 6 7.396550914343133E-5 7 0.03631706498942478 8 0.0 9 0.0 10-11 6.102154504333085E-4 12-13 6.471982050050241E-4 14-15 4.622844321464458E-4 16-17 0.004031120248317007 18-19 0.001553275692012058 20-21 0.003106551384024116 22-23 0.0017566808421564941 24-25 0.004456421925891738 26-27 0.004216034021175586 28-29 0.003272973779596836 30-31 7.581464687201711E-4 32-33 3.6982754571715664E-5 34-35 0.0011279740144373278 36-37 0.0011834481462949012 38-39 5.362499412898771E-4 40-41 0.001553275692012058 42-43 8.506033551494602E-4 44-45 0.0013868532964393374 46-47 2.0340515014443614E-4 48-49 3.6982754571715664E-5 50-51 0.03995986631473877 52-53 7.396550914343133E-5 54-55 0.1345802438864733 56-57 3.143534138595831E-4 58-59 0.0 60-61 0.0 62-63 6.102154504333085E-4 64-65 0.05928335557846021 66-67 0.05199775292783223 68-69 0.0017566808421564941 70-71 0.0028106893474503904 72-73 0.0706000784774052 74-75 0.0054919390538997765 76-77 0.001978577369586788 78-79 0.0014608188055827687 80-81 9.245688642928915E-5 82-83 1.664223955727205E-4 84-85 0.0 86-87 1.2943964100100482E-4 88-89 0.0013683619191534797 90-91 4.807758094323036E-4 92-93 0.0026442669518776697 94-95 0.004419439171320021 96-97 0.0024778445563049495 98-99 0.0 100-101 0.0011279740144373278 102-103 0.0014608188055827687 104-105 5.5474131857573496E-5 106-107 1.8491377285857832E-5 108-109 0.0 110-111 7.396550914343133E-5 112-113 1.1094826371514699E-4 114-115 1.849137728585783E-4 116-117 0.0 118-119 9.245688642928916E-4 120-121 0.002108017010587793 122-123 2.7737065928786745E-4 124-125 0.007359568159771417 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2703963.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.752476907501 #Duplication Level Percentage of deduplicated Percentage of total 1 78.84832524825293 47.90231058839615 2 11.410756095000451 13.864633923172825 3 4.146324573449148 7.556984636984199 4 1.8816263801067712 4.572538528239253 5 1.0454443953164418 3.175666824226924 6 0.637946428795547 2.3254095410174522 7 0.41889573040954614 1.7814267231849705 8 0.2854605715509191 1.3873949424919398 9 0.2085929197158872 1.1405282884296883 >10 1.04202905691808 11.152908843710781 >50 0.053470938326088825 2.18472452207297 >100 0.020263862837184052 2.0771417205339517 >500 5.552995635360829E-4 0.18933294063360714 >1k 1.8509985448711427E-4 0.23727529480504533 >5k 1.233999029914095E-4 0.45172268210041094 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6305 0.23317626757466725 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5746 0.21250290776907818 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3463 0.12807127908185134 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.6982754571715664E-5 0.0 0.0 0.0 0.0 8 3.6982754571715664E-5 0.0 0.0 0.0 0.0 9 3.6982754571715664E-5 0.0 0.0 0.0 0.0 10-11 7.396550914343133E-5 0.0 0.0 3.6982754571715664E-5 0.0 12-13 9.245688642928917E-5 0.0 0.0 3.6982754571715664E-5 3.6982754571715664E-5 14-15 1.1094826371514699E-4 0.0 0.0 5.5474131857573496E-5 5.5474131857573496E-5 16-17 1.1094826371514699E-4 0.0 0.0 7.396550914343133E-5 9.245688642928917E-5 18-19 2.0340515014443614E-4 0.0 0.0 7.396550914343133E-5 1.849137728585783E-4 20-21 2.2189652743029398E-4 0.0 0.0 1.6642239557272047E-4 1.849137728585783E-4 22-23 2.5887928200200963E-4 0.0 0.0 5.362499412898771E-4 1.849137728585783E-4 24-25 2.958620365737253E-4 0.0 0.0 9.060774870070338E-4 1.849137728585783E-4 26-27 3.143534138595832E-4 0.0 0.0 0.001312887787295906 2.0340515014443614E-4 28-29 4.992671867181614E-4 0.0 0.0 0.0018306463512999254 2.2189652743029398E-4 30-31 5.917240731474506E-4 0.0 0.0 0.0037907323436008554 2.2189652743029398E-4 32-33 5.917240731474506E-4 0.0 0.0 0.0075259905553441385 2.2189652743029398E-4 34-35 6.841809595767397E-4 0.0 0.0 0.012666593440812614 2.2189652743029398E-4 36-37 8.136206005777446E-4 0.0 0.0 0.021893790706455675 2.2189652743029398E-4 38-39 8.875861097211759E-4 0.0 0.0 0.03714917696728838 2.2189652743029398E-4 40-41 8.875861097211759E-4 0.0 0.0 0.0567870196448694 2.2189652743029398E-4 42-43 9.80042996150465E-4 0.0 0.0 0.07616598304044842 2.2189652743029398E-4 44-45 0.0010724998825797543 0.0 0.0 0.09985343734363229 2.2189652743029398E-4 46-47 0.0011649567690090435 0.0 0.0 0.1297909771694361 2.2189652743029398E-4 48-49 0.0011834481462949012 0.0 0.0 0.15996890489995608 2.2189652743029398E-4 50-51 0.0011834481462949012 0.0 0.0 0.19874532306839998 2.2189652743029398E-4 52-53 0.001201939523580759 0.0 0.0 0.2375402326141297 2.7737065928786745E-4 54-55 0.0012574136554383325 0.0 0.0 0.2771487627604372 2.958620365737253E-4 56-57 0.001331379164581764 0.0 0.0 0.3245791454986625 2.958620365737253E-4 58-59 0.001331379164581764 0.0 0.0 0.37907323436008555 2.958620365737253E-4 60-61 0.001331379164581764 0.0 0.0 0.44063102934470627 2.958620365737253E-4 62-63 0.0013683619191534795 0.0 0.0 0.5061829618230723 2.958620365737253E-4 64-65 0.0013683619191534795 0.0 0.0 0.5733251527480221 3.32844791145441E-4 66-67 0.0014053446737251952 0.0 0.0 0.6493801875247553 3.32844791145441E-4 68-69 0.0014053446737251952 0.0 1.8491377285857832E-5 0.7399879362254587 3.32844791145441E-4 70-71 0.001423836051011053 0.0 3.6982754571715664E-5 0.843743054176407 3.513361684312988E-4 72-73 0.0014608188055827687 0.0 3.6982754571715664E-5 0.9519915768078188 4.068103002888723E-4 74-75 0.0014793101828686264 0.0 3.6982754571715664E-5 1.0662128143025624 4.4379305486058797E-4 76-77 0.0014793101828686264 0.0 3.6982754571715664E-5 1.1933965072746928 4.4379305486058797E-4 78-79 0.0014793101828686264 0.0 3.6982754571715664E-5 1.3383873965731041 4.4379305486058797E-4 80-81 0.0017012067102989204 0.0 3.6982754571715664E-5 1.504347507713678 4.4379305486058797E-4 82-83 0.0017012067102989204 0.0 3.6982754571715664E-5 1.6842686087050747 4.8077580943230364E-4 84-85 0.0017196980875847784 0.0 3.6982754571715664E-5 1.868276304076646 4.8077580943230364E-4 86-87 0.0017381894648706361 0.0 3.6982754571715664E-5 2.057517059220115 5.547413185757349E-4 88-89 0.0017381894648706361 0.0 3.6982754571715664E-5 2.272682725318357 6.287068277191662E-4 90-91 0.0019231032377292146 0.0 3.6982754571715664E-5 2.5152526125542396 6.287068277191662E-4 92-93 0.0019415946150150723 0.0 3.6982754571715664E-5 2.7821941350528836 6.65689582290882E-4 94-95 0.00196008599230093 0.0 3.6982754571715664E-5 3.058214923798883 6.841809595767398E-4 96-97 0.00196008599230093 0.0 3.6982754571715664E-5 3.3359923933870395 7.951292232918867E-4 98-99 0.00196008599230093 0.0 3.6982754571715664E-5 3.6395838256662536 8.875861097211759E-4 100-101 0.0020525428787302193 0.0 3.6982754571715664E-5 3.9858718480985136 8.875861097211759E-4 102-103 0.002108017010587793 0.0 3.6982754571715664E-5 4.366960642582757 9.060774870070338E-4 104-105 0.0023853876698756602 0.0 3.6982754571715664E-5 4.751636764260458 9.245688642928916E-4 106-107 0.0024593531790190917 0.0 3.6982754571715664E-5 5.140861764750479 9.245688642928916E-4 108-109 0.0025148273108766654 0.0 3.6982754571715664E-5 5.53674366106341 9.615516188646073E-4 110-111 0.0026627583291635275 0.0 3.6982754571715664E-5 5.990688482053933 9.615516188646073E-4 112-113 0.002718232461021101 0.0 3.6982754571715664E-5 6.486331358824067 9.615516188646073E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5420 0.0 56.016674 1 GTATCAA 8805 0.0 47.285164 1 ATCAACG 11145 0.0 35.98714 3 TCAACGC 11265 0.0 35.550888 4 CAACGCA 11320 0.0 35.271263 5 TATCAAC 11840 0.0 34.580036 2 AACGCAG 11575 0.0 33.980156 6 GTACATG 9540 0.0 29.073906 1 ACGCAGA 13435 0.0 28.832893 7 CGCAGAG 13650 0.0 28.711554 8 TACATGG 9705 0.0 27.326557 2 GCAGAGT 14530 0.0 25.990345 9 ACATGGG 9695 0.0 25.44866 3 GAGTACT 9160 0.0 24.413408 12-13 CAGAGTA 13985 0.0 23.41159 10-11 GTACTTT 9760 0.0 23.27821 14-15 AGAGTAC 13750 0.0 22.968246 10-11 AGTACTT 9845 0.0 22.291882 12-13 GAGTACA 8320 0.0 20.862604 1 GTGGTAT 3205 0.0 20.658262 1 >>END_MODULE