FastQCFastQC Report
Thu 26 May 2016
SRR1364497_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364497_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3273314
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70050.045.2852671
GGTATCA46550.043.342891
ATCAACG77000.038.94263
TCAACGC78000.038.190764
CAACGCA78350.037.715425
AACGCAG78400.036.990476
TATCAAC85550.036.650152
GTACATG101000.032.5867921
TACATGG102100.030.4179482
ACGCAGA96850.029.1444347
CGCAGAG96300.029.1276058
ACATGGG105100.027.0023043
GCAGAGT107450.024.276049
ACCGCGC2951.8007995E-1024.2253138
AGAGTAC93650.021.83082210-11
CAGAGTA105800.021.74277510-11
GAGTACA79050.020.6293961
GAGTACT54050.020.35893412-13
CATGGGA60700.019.2375764
AGTACAT77550.018.9496142
Produced by FastQC (version 0.11.5)