FastQCFastQC Report
Thu 26 May 2016
SRR1364497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364497_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3273314
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51300.15672190324545704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50190.15333084452026294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55750.053.1696551
GTATCAA92400.048.023441
ATCAACG109050.039.012693
TCAACGC109700.038.4013184
CAACGCA110800.037.8026285
TATCAAC116500.037.3873632
AACGCAG112800.036.0774736
GTACATG99100.030.7538551
TACATGG98150.030.2323842
ACGCAGA135800.029.79197
CGCAGAG139700.029.3291578
ACATGGG103250.026.4307083
GCAGAGT153100.025.441519
GAGTACT95850.023.73172612-13
AGAGTAC133450.023.70735410-11
CAGAGTA144100.022.842510-11
AGTACTT102050.022.1442312-13
GTACTTT106700.021.84816714-15
GTGGTAT33600.020.945711
TGGTATC31850.020.204482
Produced by FastQC (version 0.11.5)