##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364497_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3273314 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.038753996714032 31.0 28.0 31.0 25.0 34.0 2 29.23777340029096 31.0 28.0 33.0 25.0 34.0 3 29.35618642146766 31.0 28.0 34.0 26.0 34.0 4 32.79497628397398 35.0 32.0 37.0 27.0 37.0 5 31.93107169064746 35.0 32.0 37.0 22.0 37.0 6 31.990242915895024 35.0 32.0 37.0 23.0 37.0 7 31.435568051216595 35.0 32.0 37.0 17.0 37.0 8 31.581978080929602 35.0 32.0 37.0 18.0 37.0 9 32.178888123779146 37.0 31.0 39.0 13.0 39.0 10-11 32.384525132633165 37.0 31.5 39.0 17.0 39.0 12-13 32.12533383598396 37.0 30.5 39.0 14.0 39.0 14-15 33.10589466821698 38.0 31.5 40.0 16.5 41.0 16-17 33.16717461264028 38.0 32.0 40.0 17.0 41.0 18-19 33.01517865380468 38.0 31.5 40.0 14.0 41.0 20-21 32.89107919374676 38.0 31.0 40.0 13.0 41.0 22-23 32.86129454736087 38.0 31.0 40.0 12.5 41.0 24-25 32.66333080175015 38.0 31.0 40.0 10.0 41.0 26-27 32.76809007629576 38.0 31.0 40.0 10.5 41.0 28-29 32.667805319013084 38.0 31.0 40.0 10.0 41.0 30-31 32.15649399965906 37.0 30.0 40.0 9.0 41.0 32-33 32.11790161286085 37.0 30.0 40.0 9.0 41.0 34-35 31.93167062493852 37.0 30.0 40.0 8.0 41.0 36-37 31.949417012849974 37.0 30.0 40.0 8.0 41.0 38-39 31.728315859706704 37.0 30.0 40.0 4.5 41.0 40-41 31.450355816765516 37.0 30.0 40.0 2.0 41.0 42-43 31.35532949176278 37.0 30.0 40.0 2.0 41.0 44-45 31.312477507504624 37.0 30.0 40.0 2.0 41.0 46-47 31.204656504081186 37.0 29.5 40.0 2.0 41.0 48-49 30.95605218442227 36.5 29.0 40.0 2.0 41.0 50-51 30.6267568586454 36.0 28.0 40.0 2.0 41.0 52-53 30.558593676011526 36.0 28.0 40.0 2.0 41.0 54-55 30.08649567380337 35.5 27.0 40.0 2.0 41.0 56-57 30.12691816306043 35.5 28.0 40.0 2.0 41.0 58-59 29.893143768058913 35.0 27.5 39.5 2.0 41.0 60-61 29.625139690234423 35.0 27.0 39.0 2.0 41.0 62-63 29.369179828149697 35.0 26.0 39.0 2.0 41.0 64-65 28.89558777434734 34.0 26.0 39.0 2.0 40.5 66-67 28.496329255305174 34.0 25.0 38.0 2.0 40.0 68-69 28.309957431520473 34.0 25.5 38.0 2.0 40.0 70-71 28.40579944362197 34.0 26.0 37.5 2.0 40.0 72-73 28.168589845031672 34.0 26.0 37.0 2.0 39.5 74-75 27.78519644006044 34.0 25.5 36.5 2.0 39.0 76-77 27.70087470985063 34.0 26.0 36.0 2.0 39.0 78-79 27.279926551501017 34.0 25.0 36.0 2.0 38.5 80-81 27.03415972314297 33.0 24.5 35.0 2.0 37.0 82-83 26.88144843421682 33.0 25.0 35.0 2.0 37.0 84-85 26.4191324144277 33.0 22.5 35.0 2.0 36.5 86-87 26.28174703068511 33.0 23.0 35.0 2.0 36.0 88-89 26.234445580228478 33.0 23.0 35.0 2.0 36.0 90-91 26.061559019391357 33.0 23.0 35.0 2.0 35.5 92-93 26.078322458523687 33.0 23.5 35.0 2.0 35.0 94-95 26.0958420121015 33.0 24.0 35.0 2.0 35.0 96-97 25.969166111164405 33.0 23.0 35.0 2.0 35.0 98-99 25.829873944265657 33.0 22.5 35.0 2.0 35.0 100-101 25.735945589088 33.0 21.5 35.0 2.0 35.0 102-103 24.604764315308582 30.5 19.5 34.0 2.0 35.0 104-105 25.563545232751885 32.5 21.5 35.0 2.0 35.0 106-107 25.65959559638947 33.0 20.0 35.0 2.0 35.0 108-109 25.738789343154977 33.0 23.0 35.0 2.0 35.0 110-111 25.67580439884472 33.0 21.0 35.0 2.0 35.0 112-113 25.628948521284546 33.0 21.0 35.0 2.0 35.0 114-115 25.580382908575224 33.0 20.0 35.0 2.0 35.0 116-117 25.50583078800262 33.0 19.5 35.0 2.0 35.0 118-119 25.386195152680127 33.0 19.0 35.0 2.0 35.0 120-121 25.228748907070937 33.0 18.0 35.0 2.0 35.0 122-123 24.94824694483939 33.0 4.5 35.0 2.0 35.0 124-125 24.186337149445485 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7788584498557221 1101 2 0.7586695396931518 1101 3 0.8079876561062207 1101 4 0.8523290610236813 1101 5 1.1138543998572565 1101 6 1.0446946148405871 1101 7 1.4364565708768602 1101 8 1.283976381609353 1101 9 1.9411783145494041 1101 10-11 1.6240603555414523 1101 12-13 1.7778829919883847 1101 14-15 1.7098695875598864 1101 16-17 1.639624381720104 1101 18-19 1.7527514342954902 1101 20-21 1.9844156774909791 1101 22-23 1.9658069138368646 1101 24-25 2.0061842368723575 1101 26-27 1.7180206016550557 1101 28-29 1.8197883105518216 1101 30-31 2.19702688624308 1101 32-33 2.0381429277675807 1101 34-35 2.062363580007627 1101 36-37 1.8519866153343507 1101 38-39 2.078493027280498 1101 40-41 2.2195428764258516 1101 42-43 2.1282828519719423 1101 44-45 1.996288413848763 1101 46-47 1.8242331036830102 1101 48-49 1.8657180669565996 1101 50-51 2.10935232570543 1101 52-53 1.9079545404967035 1101 54-55 2.3049089656007204 1101 56-57 1.9573285571706656 1101 58-59 2.096385298934873 1101 60-61 2.1806151340533617 1101 62-63 2.212009151661835 1101 64-65 2.3941909079276193 1101 66-67 2.4575327036348646 1101 68-69 2.4267732263447783 1101 70-71 2.0178998598298996 1101 72-73 2.1769398964405866 1101 74-75 2.252715455073151 1101 76-77 1.9778441129573743 1101 78-79 2.1769362299592387 1101 80-81 2.266510183075642 1101 82-83 2.047626937894016 1101 84-85 2.3279160513843458 1101 86-87 2.2481824883884016 1101 88-89 2.175543905212198 1101 90-91 2.0914534166712393 1101 92-93 1.966113199592698 1101 94-95 1.9084238730843026 1101 96-97 1.7946513977357377 1101 98-99 1.9411369631232986 1101 100-101 2.0927414160371 1101 102-103 1.8038300008827761 1101 104-105 1.848364491560421 1101 106-107 1.9350103423247305 1101 108-109 1.9480929241041238 1101 110-111 1.938846372821903 1101 112-113 1.8517931780000119 1101 114-115 1.8822822925030565 1101 116-117 1.9221166490201718 1101 118-119 1.880369890670206 1101 120-121 1.8050116841703279 1101 122-123 1.8207375426963353 1101 124-125 1.7834223677505534 1102 1 0.429505485783519 1102 2 -1.8915409969596269 1102 3 -0.09841392243164293 1102 4 0.3437740091975954 1102 5 0.7586250160889527 1102 6 0.5073697323940252 1102 7 1.2651651747113242 1102 8 0.9042411755315207 1102 9 1.7190498011158866 1102 10-11 1.4076469451259825 1102 12-13 1.497372829733429 1102 14-15 1.5462995848682937 1102 16-17 1.4651625313750642 1102 18-19 1.54987544517477 1102 20-21 1.7547366887348375 1102 22-23 1.6613786070238774 1102 24-25 1.8177267185212287 1102 26-27 1.5112707460747288 1102 28-29 1.6337292322694026 1102 30-31 1.8963303321581577 1102 32-33 1.5696798512965202 1102 34-35 1.7826513538533817 1102 36-37 1.1815885338796654 1102 38-39 1.2006032521935133 1102 40-41 1.4840588925976697 1102 42-43 1.2686177664671412 1102 44-45 1.2829430418980259 1102 46-47 1.0377154805068436 1102 48-49 1.1131651684050077 1102 50-51 1.2588305065065342 1102 52-53 1.1229132183797965 1102 54-55 1.484899773631291 1102 56-57 0.9694606050595489 1102 58-59 1.0382026172891123 1102 60-61 0.9876672471925119 1102 62-63 1.3182031356132597 1102 64-65 1.3334610925899888 1102 66-67 1.4331498915044882 1102 68-69 1.324694239019916 1102 70-71 0.760009993300315 1102 72-73 1.1343018807291543 1102 74-75 1.342327495916983 1102 76-77 1.2887937086021601 1102 78-79 1.1541462883274534 1102 80-81 1.1878484137567007 1102 82-83 0.8622274390789926 1102 84-85 0.866116874447286 1102 86-87 0.9507874297080932 1102 88-89 1.0423283936842687 1102 90-91 0.9276971692530047 1102 92-93 0.9255439534892496 1102 94-95 0.9315080213501332 1102 96-97 0.9970961355916579 1102 98-99 0.8341061998439443 1102 100-101 0.6442050145261504 1102 102-103 0.4491535665558608 1102 104-105 0.7374191102268775 1102 106-107 1.0589819609263813 1102 108-109 0.9195817567756954 1102 110-111 0.8533688694912058 1102 112-113 0.8838125805299484 1102 114-115 1.1513996010371557 1102 116-117 0.7199638118543987 1102 118-119 0.2985295963681516 1102 120-121 0.6102339711975233 1102 122-123 1.0165130733715486 1102 124-125 1.0977621382455212 1103 1 0.543608723920066 1103 2 0.5271227691626486 1103 3 0.41584441679660245 1103 4 0.43132881034366477 1103 5 0.6976556068628277 1103 6 0.7771790034619599 1103 7 1.079663329509419 1103 8 0.8960890903378775 1103 9 1.6637802982888772 1103 10-11 1.3592924964610376 1103 12-13 1.3186858415092928 1103 14-15 1.1658883399217004 1103 16-17 1.1142939143702364 1103 18-19 1.2679742588980503 1103 20-21 1.5485961222048061 1103 22-23 1.4544584050088147 1103 24-25 1.693746766570463 1103 26-27 1.2692986955889722 1103 28-29 1.3615068904308245 1103 30-31 1.8344453684691189 1103 32-33 1.6642761323935673 1103 34-35 1.7328175727416522 1103 36-37 1.6830062095320422 1103 38-39 1.7592699856713487 1103 40-41 1.904355408930062 1103 42-43 2.003185853771601 1103 44-45 1.8394788969877176 1103 46-47 1.6876676818109893 1103 48-49 1.7086026830607288 1103 50-51 1.8359655638551828 1103 52-53 1.6092453555060757 1103 54-55 1.8860638147624407 1103 56-57 1.752680874838365 1103 58-59 1.9444621716455437 1103 60-61 1.8766502741535582 1103 62-63 1.9571734593173034 1103 64-65 2.112596433364157 1103 66-67 2.3612386522342383 1103 68-69 2.4590581934009315 1103 70-71 2.1217388184976294 1103 72-73 2.164058310945606 1103 74-75 2.1763738492992815 1103 76-77 2.029717104243435 1103 78-79 2.1542524946025097 1103 80-81 2.098955660172095 1103 82-83 2.057364734079936 1103 84-85 2.3132971037166037 1103 86-87 2.0979662301056727 1103 88-89 2.0550263071009063 1103 90-91 2.032011011340362 1103 92-93 1.8719794861236991 1103 94-95 1.7013271966523362 1103 96-97 1.6572407726445384 1103 98-99 1.8844890787503914 1103 100-101 1.8315607131602647 1103 102-103 1.6493501245017015 1103 104-105 1.6983549507538314 1103 106-107 1.7325289843125091 1103 108-109 1.6740796492877408 1103 110-111 1.6609456870062758 1103 112-113 1.6022114768929043 1103 114-115 1.471867660273091 1103 116-117 1.5656790365415425 1103 118-119 1.5920177189207223 1103 120-121 1.5688207857256664 1103 122-123 1.7614593888985297 1103 124-125 1.600626125705901 1104 1 0.5996932365612757 1104 2 0.5670868277592902 1104 3 0.5470610806602032 1104 4 0.6420415338167444 1104 5 0.8612516043076219 1104 6 0.9100509930710956 1104 7 1.2922823495151086 1104 8 1.0269208701757186 1104 9 1.6143103761867792 1104 10-11 1.193708963277146 1104 12-13 1.2554789420128287 1104 14-15 1.2000060284397307 1104 16-17 0.9598837087718337 1104 18-19 0.9327732566557216 1104 20-21 1.4043677417718854 1104 22-23 1.3950141281682704 1104 24-25 1.56211102251293 1104 26-27 1.405744520393469 1104 28-29 1.3801645360712627 1104 30-31 1.8535274083208932 1104 32-33 1.6451655087218313 1104 34-35 1.6254479255886807 1104 36-37 1.5523178895185588 1104 38-39 1.7179806297787472 1104 40-41 1.7315470681975036 1104 42-43 1.7150777297390754 1104 44-45 1.4855367164804356 1104 46-47 1.541823206132971 1104 48-49 1.2172123608373617 1104 50-51 1.5790218771502538 1104 52-53 1.3589267755010255 1104 54-55 1.5866723350155603 1104 56-57 1.6245773301836515 1104 58-59 1.8016642687619182 1104 60-61 1.8246063725385824 1104 62-63 1.8424059913962552 1104 64-65 1.9994298271716282 1104 66-67 2.0443775546826757 1104 68-69 2.039864540537909 1104 70-71 1.8685398359548273 1104 72-73 2.0127862097607476 1104 74-75 2.162835243242494 1104 76-77 2.0084539119621496 1104 78-79 2.012331609693682 1104 80-81 1.9710363534332096 1104 82-83 1.8877084820172563 1104 84-85 2.075792726644373 1104 86-87 1.9897475894275942 1104 88-89 1.8253417397159915 1104 90-91 1.9210451355362217 1104 92-93 1.7480361085640688 1104 94-95 1.6924119908432402 1104 96-97 1.7309711979486586 1104 98-99 1.745234488091949 1104 100-101 1.6717600801768846 1104 102-103 1.585413246824416 1104 104-105 1.5373402419265574 1104 106-107 1.6302896946679795 1104 108-109 1.5189257982286506 1104 110-111 1.6075461888732647 1104 112-113 1.5489056919230322 1104 114-115 1.5271751330494752 1104 116-117 1.5769539830564234 1104 118-119 1.530972543060308 1104 120-121 1.4641603924585418 1104 122-123 1.5622865856294226 1104 124-125 1.3341575941982526 1105 1 0.34990463961421625 1105 2 0.46224242488924006 1105 3 0.3724798843738455 1105 4 0.5290365817918854 1105 5 0.6678393709757948 1105 6 0.7945873146952067 1105 7 1.0802145993291212 1105 8 0.8872572884347072 1105 9 1.5771283992078864 1105 10-11 1.2069676901448574 1105 12-13 1.394891875601843 1105 14-15 1.318283443648852 1105 16-17 1.260722220394051 1105 18-19 1.4268104520436538 1105 20-21 1.5924766819118261 1105 22-23 1.520270180594558 1105 24-25 1.6882256612084205 1105 26-27 1.421045284119053 1105 28-29 1.3772246108580575 1105 30-31 1.7700831908032058 1105 32-33 1.6445115630393303 1105 34-35 1.8260098333457506 1105 36-37 1.5364953331376334 1105 38-39 1.679437573498074 1105 40-41 1.7582650784459055 1105 42-43 1.8442099290397458 1105 44-45 1.6579027111833717 1105 46-47 1.5928097891719908 1105 48-49 1.5936246430261143 1105 50-51 1.7108128274355678 1105 52-53 1.648540182021975 1105 54-55 1.9351077624668669 1105 56-57 1.638115198740909 1105 58-59 1.6848491039085651 1105 60-61 1.5975160695276642 1105 62-63 1.4630335535826404 1105 64-65 1.8147074199524766 1105 66-67 2.0396677058952157 1105 68-69 2.0149936143634477 1105 70-71 1.8294563332495741 1105 72-73 1.9822826051002984 1105 74-75 2.118192176468611 1105 76-77 1.8390581026092931 1105 78-79 2.0182026461191356 1105 80-81 1.9957811672104668 1105 82-83 1.8998274103958224 1105 84-85 2.1478090790475832 1105 86-87 2.0346755155942304 1105 88-89 1.8735453399466486 1105 90-91 1.949869331093371 1105 92-93 1.7946989112537644 1105 94-95 1.608618385568093 1105 96-97 1.6770295501301469 1105 98-99 1.7420808613471088 1105 100-101 1.7722389311715645 1105 102-103 1.6195844066686789 1105 104-105 1.2461763690575793 1105 106-107 1.497541741434766 1105 108-109 1.5415074396220518 1105 110-111 1.6385085774796835 1105 112-113 1.7039566767394732 1105 114-115 1.5936143313735371 1105 116-117 1.5737803158568404 1105 118-119 1.6857204896070392 1105 120-121 1.6504968066144663 1105 122-123 1.7279467402437163 1105 124-125 1.5671286024416986 1106 1 0.531225577522175 1106 2 0.4469191262714993 1106 3 0.47556361944796066 1106 4 0.3766308634497051 1106 5 0.6522531877696416 1106 6 0.7227996655136053 1106 7 0.9624215293499212 1106 8 0.6093094285500307 1106 9 1.3272912733550513 1106 10-11 1.0323371032295299 1106 12-13 0.840164617590041 1106 14-15 0.9632053690884845 1106 16-17 1.0391779325270818 1106 18-19 1.0410869386699062 1106 20-21 0.9288788298426311 1106 22-23 0.8891464138507246 1106 24-25 0.7137690229555318 1106 26-27 0.7761972273952935 1106 28-29 0.7044537161669666 1106 30-31 1.0819406912928287 1106 32-33 0.9510078474390582 1106 34-35 0.9061004729244928 1106 36-37 0.9267190460689001 1106 38-39 1.0561935483303522 1106 40-41 1.18192035118172 1106 42-43 1.3113467683452846 1106 44-45 1.2419773960462273 1106 46-47 1.1778329900497972 1106 48-49 1.19613793244444 1106 50-51 1.4407029673173568 1106 52-53 0.9882076515523792 1106 54-55 1.5806200970364728 1106 56-57 1.296867834537327 1106 58-59 1.2969512708683908 1106 60-61 1.5387938384499478 1106 62-63 1.6607991427005189 1106 64-65 1.7418831374482338 1106 66-67 1.7832822902132115 1106 68-69 1.6186484515325468 1106 70-71 1.5652165454354332 1106 72-73 1.5468734025333575 1106 74-75 1.7999750931097935 1106 76-77 1.4100912328635111 1106 78-79 1.2886883755945249 1106 80-81 1.4198654808778066 1106 82-83 1.3009853536807157 1106 84-85 1.6010644304943114 1106 86-87 1.6487708636182106 1106 88-89 1.373438803727577 1106 90-91 1.5299527884550983 1106 92-93 1.518573751314289 1106 94-95 1.3352042422536705 1106 96-97 1.3017579773759884 1106 98-99 1.3119257208258759 1106 100-101 1.052947272304941 1106 102-103 0.8887269457198599 1106 104-105 1.0845421196749427 1106 106-107 1.018514587402933 1106 108-109 0.9686873274373689 1106 110-111 1.0247565471518811 1106 112-113 0.6744337875544382 1106 114-115 0.8424871094427218 1106 116-117 1.1040519704728275 1106 118-119 0.7809074543001877 1106 120-121 0.7184881907342593 1106 122-123 1.0363634954138305 1106 124-125 1.0113013068043486 1107 1 0.4395616905250037 1107 2 0.46132198377174305 1107 3 0.28142605823732225 1107 4 0.30687737380629443 1107 5 0.35061132965323694 1107 6 0.6696979018133149 1107 7 0.8136398353266792 1107 8 0.8492339373592692 1107 9 1.4440342651133307 1107 10-11 0.9124196130857669 1107 12-13 1.1787536110253853 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-4.2575172434320905 2216 102-103 -4.264382062395072 2216 104-105 -4.0749598449218105 2216 106-107 -4.157357328990315 2216 108-109 -4.207776584068014 2216 110-111 -3.8266366456216403 2216 112-113 -3.966210833096106 2216 114-115 -3.850885161388078 2216 116-117 -3.789850100117633 2216 118-119 -3.9786222745719577 2216 120-121 -3.9301435115111865 2216 122-123 -4.047978719112031 2216 124-125 -3.9945625250369687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 153594.0 3 51363.0 4 24905.0 5 19928.0 6 19445.0 7 22904.0 8 25622.0 9 27328.0 10 29491.0 11 32096.0 12 34719.0 13 37210.0 14 37929.0 15 31913.0 16 23933.0 17 20625.0 18 18120.0 19 16940.0 20 16309.0 21 17571.0 22 21653.0 23 29758.0 24 40594.0 25 54486.0 26 67230.0 27 77113.0 28 87481.0 29 99811.0 30 111306.0 31 126719.0 32 147806.0 33 175855.0 34 213983.0 35 269096.0 36 376261.0 37 510331.0 38 200737.0 39 1149.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.47789289674707 20.59792272070863 14.455538381836009 24.46864600070829 2 15.341741756420499 20.91711588134928 39.289158816547804 24.45198354568242 3 18.885264291785024 24.942214526317976 29.988568160585878 26.183953021311122 4 12.717197300963173 17.83215242311026 37.5306041004598 31.920046175466776 5 12.844658349305933 38.59981046731234 34.91895369646787 13.636577486913874 6 30.955861484510017 38.251536900427055 17.458571738873417 13.334029876189508 7 27.104521785584833 32.56493071876568 21.469756047170772 18.86079144847871 8 24.95415960705267 37.212531397843286 19.601816385473562 18.231492609630486 9 24.8886907886014 16.606961629712274 20.4662003095334 38.03814727215293 10-11 24.353010202109893 27.405761200454528 27.76636131162696 20.47486728580862 12-13 25.121414428833592 24.52374413167413 28.205174422210295 22.149667017281985 14-15 22.02482695581855 26.068456360666325 26.19256669956927 25.71414998394585 16-17 21.656880027618485 29.345405218794014 26.808353981003126 22.189360772584376 18-19 21.59604297117495 28.01527771667669 28.944946084397717 21.44373322775065 20-21 22.22989394187043 27.18734390299416 29.15463589967631 21.428126255459098 22-23 21.84266068240118 27.07117893971146 28.93590026183453 22.150260116052838 24-25 21.683776853930354 27.226056876446908 29.03369004910411 22.05647622051863 26-27 21.75108469156234 27.505086436967517 28.85720260707868 21.88662626439146 28-29 21.59057337520473 27.509557169947303 29.00416626313662 21.89570319171135 30-31 21.70784823885826 27.203819644983934 29.037205244817255 22.05112687134055 32-33 21.60209268134344 27.296813243465145 28.981421724934602 22.11967235025681 34-35 21.941514143349007 27.331735542744717 28.903588765974085 21.82316154793219 36-37 21.928464261917657 27.1640953870942 28.883658649920125 22.023781701068014 38-39 21.761181732238434 27.43834846614109 28.917682831359766 21.882786970260714 40-41 21.957375827157026 27.23908274930803 28.758195193782342 22.045346229752603 42-43 21.83979505625612 27.269640302099862 28.673549551162385 22.217015090481638 44-45 21.991475222727523 27.398693421465058 28.530615449570185 22.079215906237227 46-47 22.04933906331483 27.566951363284065 28.113355628814755 22.27035394458635 48-49 22.077910373665823 27.629870353044506 27.982555897122563 22.30966337616711 50-51 22.088989444235434 27.672826615477568 27.760279408847843 22.47790453143915 52-53 22.261126079812446 27.77497650315285 27.686824184010593 22.27707323302411 54-55 22.19108604104663 27.5752421879075 27.698498636117996 22.535173134927877 56-57 22.16913114753097 27.841653056573385 27.63951796746164 22.349697828434007 58-59 22.254815761640955 27.682052500921085 27.65104417113665 22.41208756630131 60-61 22.20779919066732 27.6587427909452 27.853499542054323 22.27995847633316 62-63 22.100291021826944 27.648478871742043 27.93132850272539 22.319901603705624 64-65 22.08650883004873 27.498591526907106 27.804527472944017 22.610372170100142 66-67 21.992934819321928 27.729825461243447 27.544301042598633 22.732938676835996 68-69 22.013205191891384 27.572986913955454 27.799580723548225 22.614227170604945 70-71 22.010950093699954 27.447380464583127 27.941344501153914 22.600324940563006 72-73 22.11795238504323 27.465180573087856 27.736310004411568 22.680557037457348 74-75 21.97158179654849 27.623418246987576 27.646347240279507 22.758652716184432 76-77 22.00989900279813 27.52250891237143 27.721561294110852 22.746030790719583 78-79 22.059562806889446 27.52891415076831 27.673708305233223 22.73781473710902 80-81 21.885312097740833 27.58877521358735 27.72001853170316 22.80589415696866 82-83 21.97940646920189 27.649585228650086 27.701978685486356 22.669029616661668 84-85 21.931892855953585 27.480155426627395 27.60040057275305 22.987551144665968 86-87 21.9150421752439 27.51984881342459 27.714957337481227 22.85015167385028 88-89 21.977039495330818 27.414318940094518 27.703204101827133 22.905437462747532 90-91 21.970541035206377 27.492599973299107 27.708819145455394 22.82803984603912 92-93 21.992992871279142 27.518026492421267 27.756780900136196 22.732199736163395 94-95 21.875927996627134 27.478858480132473 27.73652839135031 22.90868513189009 96-97 21.936050245232074 27.39308254303247 27.720007723267504 22.95085948846795 98-99 21.621283506562463 27.280670293164665 27.701848951857354 23.396197248415522 100-101 21.716598819225393 27.381154959482473 27.80438551603516 23.097860705256966 102-103 21.797101396144004 27.257581152521848 27.817908446473403 23.12740900486074 104-105 21.793186249687814 27.211115973674943 27.828030211032722 23.167667565604514 106-107 21.37790409224663 27.270529054364513 27.895568769490964 23.4559980838979 108-109 21.58474561255046 27.287009434475273 27.635020654908143 23.493224298066117 110-111 21.434378138072596 27.28077445836886 27.746297551680914 23.538549851877626 112-113 21.596155206221717 27.245361307875104 27.7866330696807 23.371850416222482 114-115 21.515621801784736 27.279741301618238 27.664596387143288 23.540040509453732 116-117 21.412626469687908 27.251220017389105 27.708325568521687 23.6278279444013 118-119 21.40171552221634 27.16153385784146 27.66130641076911 23.775444209173095 120-121 21.262660045908635 27.236816237609183 27.777534220293315 23.722989496188866 122-123 21.363916037941056 27.219144786526577 27.683295817538816 23.733643357993554 124-125 21.232040495463263 27.156026565982028 27.639962383801326 23.971970554753383 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 154.0 1 126.5 2 160.5 3 240.5 4 206.0 5 182.5 6 350.5 7 783.5 8 1281.5 9 1518.5 10 1338.5 11 1101.5 12 1115.0 13 1228.0 14 1399.0 15 1654.0 16 1909.5 17 2229.0 18 2702.0 19 3183.5 20 3880.0 21 4876.0 22 6179.5 23 7927.0 24 10378.0 25 13769.0 26 18640.0 27 24201.5 28 29509.5 29 35508.5 30 42175.0 31 49498.0 32 57261.5 33 64829.5 34 73175.5 35 80839.0 36 88201.0 37 95606.5 38 99939.0 39 103514.0 40 107816.5 41 111648.0 42 115351.0 43 119205.5 44 123078.5 45 125531.0 46 126000.5 47 125996.5 48 126758.5 49 125339.5 50 121056.5 51 116279.0 52 110977.5 53 105915.0 54 100773.5 55 92561.0 56 82718.5 57 73017.5 58 65057.5 59 57699.0 60 48790.0 61 39451.0 62 30694.5 63 23405.0 64 17282.5 65 12734.5 66 9323.0 67 6857.5 68 5037.5 69 3749.5 70 2762.0 71 2004.0 72 1544.0 73 1270.5 74 966.5 75 678.0 76 482.5 77 300.0 78 181.5 79 114.0 80 67.5 81 39.0 82 27.0 83 23.0 84 15.5 85 12.0 86 9.0 87 6.5 88 4.5 89 2.0 90 1.5 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1057032719745188 2 2.749507074481703E-4 3 0.0 4 0.17651835418172532 5 0.0 6 1.5275039302676126E-4 7 0.0393485012436937 8 0.0 9 0.0 10-11 6.262766114097212E-4 12-13 6.415516507123973E-4 14-15 5.193513362909883E-4 16-17 0.004399211319170725 18-19 0.0015275039302676127 20-21 0.0033299585679833956 22-23 0.001939929991439868 24-25 0.005086588087791151 26-27 0.004536686672894809 28-29 0.0035743591968262134 30-31 7.790270044364825E-4 32-33 4.5825117908028375E-5 34-35 0.0013442034586354991 36-37 0.0012525532228194424 38-39 5.346263755936644E-4 40-41 0.0016497042446890215 42-43 7.790270044364825E-4 44-45 9.77602515371272E-4 46-47 1.5275039302676126E-4 48-49 4.5825117908028375E-5 50-51 0.03858474927855989 52-53 7.637519651338063E-5 54-55 0.1380710802568895 56-57 2.44400628842818E-4 58-59 0.0 60-61 0.0 62-63 6.11001572107045E-4 64-65 0.062352710433523945 66-67 0.05428748968171095 68-69 0.0017108044018997262 70-71 0.0030550078605352253 72-73 0.07231203605886878 74-75 0.005056038009185798 76-77 0.0016802543232943739 78-79 0.0014205786551488796 80-81 1.3747535372408515E-4 82-83 1.3747535372408515E-4 84-85 3.055007860535225E-5 86-87 2.2912558954014188E-4 88-89 0.0012525532228194424 90-91 4.5825117908028377E-4 92-93 0.002321805974006771 94-95 0.004093710533117202 96-97 0.0026884069172709983 98-99 0.0 100-101 0.001191453065608738 102-103 0.001481678812359584 104-105 1.9857551093478966E-4 106-107 6.11001572107045E-5 108-109 0.0 110-111 2.1385055023746577E-4 112-113 1.3747535372408515E-4 114-115 1.22200314421409E-4 116-117 0.0 118-119 7.637519651338063E-4 120-121 0.0022454307774933905 122-123 7.943020437391587E-4 124-125 0.007820820122970177 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3273314.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.33955224604983 #Duplication Level Percentage of deduplicated Percentage of total 1 80.09468388321059 51.53256098334674 2 11.342708127985118 14.595695244243851 3 3.796765468624573 7.328465707037059 4 1.686238898208274 4.339674227623711 5 0.9326750970478254 3.0003949067549085 6 0.5372511679727074 2.0739899754618785 7 0.35588608923477827 1.6028286142374362 8 0.23534159685836678 1.211341837339015 9 0.17328867565711176 1.0034384220980572 >10 0.780636200390953 8.775212884980165 >50 0.045388119617960826 2.008634971217989 >100 0.018608822488124982 1.9464904705910109 >500 3.3590626689245705E-4 0.13279066403192363 >1k 9.597321833963757E-5 0.13513711520487026 >5k 9.597321833963757E-5 0.3133439758314436 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5130 0.15672190324545704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5019 0.15333084452026294 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.055007860535225E-5 0.0 2 9.165023581605675E-5 0.0 0.0 3.055007860535225E-5 0.0 3 9.165023581605675E-5 0.0 0.0 3.055007860535225E-5 0.0 4 9.165023581605675E-5 0.0 0.0 3.055007860535225E-5 0.0 5 9.165023581605675E-5 0.0 0.0 3.055007860535225E-5 0.0 6 1.22200314421409E-4 0.0 0.0 3.055007860535225E-5 3.055007860535225E-5 7 1.833004716321135E-4 0.0 0.0 3.055007860535225E-5 3.055007860535225E-5 8 1.833004716321135E-4 0.0 0.0 3.055007860535225E-5 3.055007860535225E-5 9 1.833004716321135E-4 0.0 0.0 3.055007860535225E-5 3.055007860535225E-5 10-11 1.833004716321135E-4 0.0 0.0 3.055007860535225E-5 3.055007860535225E-5 12-13 1.833004716321135E-4 0.0 0.0 7.637519651338063E-5 4.582511790802838E-5 14-15 2.1385055023746577E-4 0.0 0.0 1.22200314421409E-4 9.165023581605676E-5 16-17 2.1385055023746577E-4 0.0 0.0 1.5275039302676126E-4 1.6802543232943736E-4 18-19 2.44400628842818E-4 0.0 0.0 1.9857551093478964E-4 1.833004716321135E-4 20-21 2.7495070744817024E-4 0.0 0.0 3.6660094326422706E-4 1.9857551093478964E-4 22-23 2.7495070744817024E-4 0.0 0.0 5.499014148963406E-4 2.291255895401419E-4 24-25 3.971510218695793E-4 0.0 0.0 6.873767686204257E-4 2.5967566814549413E-4 26-27 3.971510218695793E-4 0.0 0.0 8.095770830418347E-4 3.207758253561987E-4 28-29 5.040762969883122E-4 0.0 0.0 0.001099802829792681 3.66600943264227E-4 30-31 5.193513362909883E-4 0.0 0.0 0.0026731318779683218 3.818759825669031E-4 32-33 5.193513362909883E-4 0.0 0.0 0.006461341625032001 3.971510218695793E-4 34-35 5.499014148963405E-4 0.0 0.0 0.011242428926769629 3.971510218695793E-4 36-37 6.721017293177496E-4 0.0 0.0 0.01994920132929502 3.971510218695793E-4 38-39 7.026518079231018E-4 0.0 0.0 0.034475763706140014 3.971510218695793E-4 40-41 7.026518079231018E-4 0.0 0.0 0.053462637559366435 3.971510218695793E-4 42-43 7.637519651338062E-4 0.0 0.0 0.07394646526425513 4.1242606117225544E-4 44-45 8.859522795552153E-4 0.0 0.0 0.09664517366803185 4.4297613977760766E-4 46-47 9.62327476068596E-4 0.0 0.0 0.12403331913773014 4.5825117908028377E-4 48-49 0.0010387026725819765 0.0 0.0 0.1536210702670138 4.88801257685636E-4 50-51 0.0010387026725819765 0.0 0.0 0.18840233475920734 4.88801257685636E-4 52-53 0.0010387026725819765 0.0 0.0 0.2252151794786568 5.804514935016928E-4 54-55 0.0010692527511873288 0.0 0.0 0.26338750269604444 5.804514935016928E-4 56-57 0.001099802829792681 0.0 0.0 0.31118310067411803 5.95726532804369E-4 58-59 0.001099802829792681 0.0 0.0 0.36467628831208987 6.11001572107045E-4 60-61 0.001099802829792681 0.0 0.0 0.4245238922999749 6.11001572107045E-4 62-63 0.001099802829792681 0.0 0.0 0.48811388091701563 6.415516507123972E-4 64-65 0.0011303529083980333 0.0 0.0 0.5534299489752588 6.415516507123972E-4 66-67 0.00122200314421409 0.0 0.0 0.6287053426588467 6.415516507123972E-4 68-69 0.0012525532228194424 0.0 0.0 0.7158494418806141 6.415516507123972E-4 70-71 0.0012525532228194424 0.0 0.0 0.8185893562304136 7.484769258311302E-4 72-73 0.0012678282621221184 0.0 0.0 0.9261409079605563 9.62327476068596E-4 74-75 0.0012983783407274707 0.0 0.0 1.03467006220607 0.0010081525939766244 76-77 0.0013136533800301468 0.0 0.0 1.1553886978151193 0.0010081525939766244 78-79 0.0013289284193328229 1.5275039302676126E-5 0.0 1.2980422898628117 0.0010234276332793005 80-81 0.001573329048175641 3.055007860535225E-5 0.0 1.4625239130740284 0.0010539777118846526 82-83 0.0016191541660836694 3.055007860535225E-5 0.0 1.6314963978402317 0.0011303529083980333 84-85 0.0016649792839916978 3.055007860535225E-5 0.0 1.8047458936111842 0.0011609029870033856 86-87 0.0017260794412024022 3.055007860535225E-5 0.0 1.9922622760908362 0.0011761780263060617 88-89 0.0017413544805050783 4.582511790802838E-5 0.0 2.2034244194110313 0.001206728104911414 90-91 0.001833004716321135 6.11001572107045E-5 0.0 2.4485124250224697 0.00122200314421409 92-93 0.0018941048735318395 6.11001572107045E-5 0.0 2.711472226618039 0.0012525532228194424 94-95 0.0018941048735318395 6.11001572107045E-5 0.0 2.9811683205460886 0.0012983783407274707 96-97 0.0019093799128345156 6.11001572107045E-5 0.0 3.251719816675088 0.001435853694451556 98-99 0.002107955423769305 6.11001572107045E-5 0.0 3.553065792038283 0.0015275039302676127 100-101 0.0022148806988880384 6.11001572107045E-5 0.0 3.8987857565757515 0.0015275039302676127 102-103 0.0022912558954014187 6.11001572107045E-5 0.0 4.271863316504314 0.001573329048175641 104-105 0.002550931563546913 6.11001572107045E-5 0.0 4.645017251629389 0.001588604087478317 106-107 0.002566206602849589 6.11001572107045E-5 0.0 5.021088719261275 0.001588604087478317 108-109 0.0025814816421522654 6.11001572107045E-5 0.0 5.414741757130541 0.001588604087478317 110-111 0.0026273067600602936 6.11001572107045E-5 0.0 5.864087588297364 0.0016038791267809932 112-113 0.0026273067600602936 6.11001572107045E-5 0.0 6.345999803257493 0.0016191541660836694 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5575 0.0 53.169655 1 GTATCAA 9240 0.0 48.02344 1 ATCAACG 10905 0.0 39.01269 3 TCAACGC 10970 0.0 38.401318 4 CAACGCA 11080 0.0 37.802628 5 TATCAAC 11650 0.0 37.387363 2 AACGCAG 11280 0.0 36.077473 6 GTACATG 9910 0.0 30.753855 1 TACATGG 9815 0.0 30.232384 2 ACGCAGA 13580 0.0 29.7919 7 CGCAGAG 13970 0.0 29.329157 8 ACATGGG 10325 0.0 26.430708 3 GCAGAGT 15310 0.0 25.44151 9 GAGTACT 9585 0.0 23.731726 12-13 AGAGTAC 13345 0.0 23.707354 10-11 CAGAGTA 14410 0.0 22.8425 10-11 AGTACTT 10205 0.0 22.14423 12-13 GTACTTT 10670 0.0 21.848167 14-15 GTGGTAT 3360 0.0 20.94571 1 TGGTATC 3185 0.0 20.20448 2 >>END_MODULE