FastQCFastQC Report
Thu 26 May 2016
SRR1364496_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364496_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262827
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32450.1434046880296196No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32650.058.7074781
GTATCAA59150.053.7411881
ATCAACG69400.042.6961753
TCAACGC71000.041.620694
CAACGCA70000.041.107915
TATCAAC75850.041.10412
AACGCAG72200.038.9269566
GTACATG90450.033.4988561
CGCAGAG84750.032.252038
ACGCAGA86300.032.086797
TACATGG90800.032.042952
ACATGGG93650.029.1624473
GCAGAGT92000.027.3802019
CATGGGA58150.024.3844644
GAGTACT46250.024.30638312-13
CAGAGTA91550.023.82540710-11
AGAGTAC87250.023.0214710-11
TAACGCA3307.2577677E-1021.6647284
GAGTACA69250.021.6621481
GTACTTT53300.021.40915914-15
Produced by FastQC (version 0.11.5)