FastQCFastQC Report
Thu 26 May 2016
SRR1364496_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364496_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262827
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67220.2970620378844693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62020.27408193379343626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40240.17783065165830175No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46800.062.8657531
GTATCAA85450.051.751051
ATCAACG107900.038.7240033
TATCAAC114250.038.449882
TCAACGC109500.037.8864
CAACGCA108900.037.8021285
AACGCAG111300.036.6128436
GTACATG97900.031.8810081
TACATGG97550.031.0398862
CGCAGAG133500.030.602048
ACGCAGA132550.030.5187857
GTGGTAT25300.028.5415731
ACATGGG97050.028.3132083
GCAGAGT144650.027.5442689
GAGTACT89150.026.58399612-13
CCGCGTA701.0599644E-425.48804310-11
TGGTATC26000.025.4468062
AGAGTAC137800.024.7512710-11
AGTACTT97600.024.37381212-13
GTACTTT100450.024.30419714-15
Produced by FastQC (version 0.11.5)