##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364496_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2262827 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.90430642731415 31.0 28.0 31.0 25.0 34.0 2 29.08606314137139 31.0 28.0 33.0 25.0 34.0 3 29.20515001809683 31.0 28.0 34.0 25.0 34.0 4 32.634480232028345 35.0 32.0 37.0 26.0 37.0 5 31.74629523158421 35.0 32.0 37.0 20.0 37.0 6 31.79900098416715 35.0 32.0 37.0 21.0 37.0 7 31.23816845034994 35.0 31.0 37.0 17.0 37.0 8 31.374436932209136 35.0 32.0 37.0 17.0 37.0 9 31.927709011780397 37.0 30.0 39.0 11.0 39.0 10-11 32.123719798287716 37.0 31.0 39.0 15.5 39.0 12-13 31.862074961983396 37.0 30.5 39.0 12.5 39.0 14-15 32.81235816967006 38.0 31.0 40.0 13.0 41.0 16-17 32.88128898055397 38.0 31.0 40.0 13.0 41.0 18-19 32.754405219665486 38.0 31.0 40.0 11.0 41.0 20-21 32.63946647269102 38.0 30.5 40.0 10.5 41.0 22-23 32.611385448379394 38.0 31.0 40.0 10.0 41.0 24-25 32.41079874864495 37.0 30.0 40.0 10.0 41.0 26-27 32.504653913003516 37.0 31.0 40.0 10.0 41.0 28-29 32.39235677318682 37.0 30.5 40.0 9.5 41.0 30-31 31.851522012067207 37.0 30.0 40.0 8.0 41.0 32-33 31.772703127547974 37.0 30.0 40.0 8.0 41.0 34-35 31.557815511305108 37.0 30.0 40.0 4.5 41.0 36-37 31.530754450075058 37.0 30.0 40.0 2.0 41.0 38-39 31.29542581028068 36.0 30.0 40.0 2.0 41.0 40-41 31.003567219235055 36.0 29.5 40.0 2.0 41.0 42-43 30.881888451923192 36.0 28.5 40.0 2.0 41.0 44-45 30.80319021295044 36.0 29.0 40.0 2.0 41.0 46-47 30.643812142952157 36.0 28.5 40.0 2.0 41.0 48-49 30.354753589205007 36.0 28.0 40.0 2.0 41.0 50-51 29.995823808006534 35.0 27.0 40.0 2.0 41.0 52-53 29.902601701323167 35.0 27.0 40.0 2.0 41.0 54-55 29.4361449196072 35.0 26.0 39.5 2.0 41.0 56-57 29.458400487531748 35.0 26.0 39.0 2.0 41.0 58-59 29.21912589870989 35.0 26.0 39.0 2.0 41.0 60-61 28.943929650830576 35.0 26.0 39.0 2.0 41.0 62-63 28.67157321350682 34.0 25.5 39.0 2.0 40.5 64-65 28.197571002997577 34.0 24.0 38.0 2.0 40.0 66-67 27.804840803119284 34.0 22.5 38.0 2.0 40.0 68-69 27.62028073732548 34.0 22.5 37.5 2.0 40.0 70-71 27.704321187611782 34.0 24.5 37.0 2.0 40.0 72-73 27.471727180204233 34.0 23.5 37.0 2.0 39.0 74-75 27.104016568655048 34.0 22.5 36.0 2.0 39.0 76-77 27.026493187503952 33.0 23.5 36.0 2.0 39.0 78-79 26.623746092829897 33.0 22.0 35.5 2.0 37.5 80-81 26.390226473345066 33.0 22.0 35.0 2.0 37.0 82-83 26.25783743078901 33.0 22.0 35.0 2.0 37.0 84-85 25.817591225489178 33.0 20.0 35.0 2.0 36.0 86-87 25.68728342909113 33.0 20.0 35.0 2.0 36.0 88-89 25.638892412013824 33.0 20.0 35.0 2.0 36.0 90-91 25.471089261353164 33.0 20.0 35.0 2.0 35.0 92-93 25.48638804468923 33.0 20.0 35.0 2.0 35.0 94-95 25.49592788136256 33.0 20.0 35.0 2.0 35.0 96-97 25.37839326647596 33.0 20.0 35.0 2.0 35.0 98-99 25.24032614954656 33.0 18.5 35.0 2.0 35.0 100-101 25.139863100449126 33.0 18.0 35.0 2.0 35.0 102-103 24.009546465549512 30.5 16.5 34.0 2.0 35.0 104-105 24.955734353532108 32.0 18.0 35.0 2.0 35.0 106-107 25.052371436260923 33.0 18.0 35.0 2.0 35.0 108-109 25.125037176947245 33.0 18.0 35.0 2.0 35.0 110-111 25.051265960676623 33.0 17.5 35.0 2.0 35.0 112-113 25.00346933283013 33.0 17.0 35.0 2.0 35.0 114-115 24.950797387515706 33.0 15.5 35.0 2.0 35.0 116-117 24.86961442478811 33.0 8.5 35.0 2.0 35.0 118-119 24.745894184575313 33.0 4.5 35.0 2.0 35.0 120-121 24.584572307118485 33.0 2.0 35.0 2.0 35.0 122-123 24.301477974233116 33.0 2.0 35.0 2.0 35.0 124-125 23.54598208347346 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7807328423894546 1101 2 0.865078952375903 1101 3 0.6940354786182716 1101 4 0.8189280442468956 1101 5 0.9283849743039525 1101 6 0.9863698523875577 1101 7 1.3868205076704392 1101 8 1.1778223655600435 1101 9 1.9552013296062043 1101 10-11 1.5527128365014562 1101 12-13 1.6911363476225532 1101 14-15 1.6238955225246912 1101 16-17 1.5767870605324532 1101 18-19 1.6303491565392108 1101 20-21 1.820287214957382 1101 22-23 1.8358665025375984 1101 24-25 2.1012688091165614 1101 26-27 1.8224079374495687 1101 28-29 1.8475946336573443 1101 30-31 2.3361842610682153 1101 32-33 2.191306247627736 1101 34-35 2.2857397653615763 1101 36-37 2.0438110994972902 1101 38-39 2.2070847682797883 1101 40-41 2.3577464387790847 1101 42-43 2.274787791389805 1101 44-45 2.1878924124383445 1101 46-47 1.951368412733892 1101 48-49 1.9107827212679034 1101 50-51 2.0736406291695637 1101 52-53 2.0880036508122792 1101 54-55 2.499426601019639 1101 56-57 2.058169925587265 1101 58-59 2.2795312365897473 1101 60-61 2.2544696315054082 1101 62-63 2.3199405857597704 1101 64-65 2.478775490086793 1101 66-67 2.5151345672387393 1101 68-69 2.4354355539177917 1101 70-71 2.22073663117844 1101 72-73 2.3216973995146475 1101 74-75 2.4377544675211027 1101 76-77 2.127374568984184 1101 78-79 2.211596057915905 1101 80-81 2.2539550872996514 1101 82-83 2.0538823068904755 1101 84-85 2.2761369579945026 1101 86-87 2.1722351048272905 1101 88-89 2.1038760231530915 1101 90-91 2.091166619641367 1101 92-93 2.0350778733623898 1101 94-95 2.0268546663027998 1101 96-97 1.9405289026357728 1101 98-99 2.0417891861224646 1101 100-101 2.067806115872987 1101 102-103 1.8580955777369468 1101 104-105 1.8353169887140623 1101 106-107 1.9448552188424593 1101 108-109 1.9674553717838457 1101 110-111 1.9441604884598718 1101 112-113 1.8614481836406895 1101 114-115 1.8718191939037006 1101 116-117 1.9080185592214534 1101 118-119 1.846049426855778 1101 120-121 1.829164934409043 1101 122-123 2.0039578357322405 1101 124-125 1.8704986904329886 1102 1 0.4900618131947141 1102 2 -1.7036476309910462 1102 3 0.016545990684114997 1102 4 0.5176714103987905 1102 5 1.0219074169212732 1102 6 0.7487770781151859 1102 7 1.4430527973502727 1102 8 1.1980850468547963 1102 9 1.9994003126790005 1102 10-11 1.7238662836124448 1102 12-13 1.9495612724652318 1102 14-15 1.8827616802297413 1102 16-17 1.9120677613257726 1102 18-19 1.9473506488956573 1102 20-21 2.037208777433001 1102 22-23 1.940198984281544 1102 24-25 2.2264403305067333 1102 26-27 1.9241886390729022 1102 28-29 1.8564693404566057 1102 30-31 2.2257993526265274 1102 32-33 1.9684204054257535 1102 34-35 2.224266631927648 1102 36-37 1.652766898190194 1102 38-39 1.5235362965756316 1102 40-41 1.7934662974119213 1102 42-43 1.69972209616585 1102 44-45 1.6524650490287982 1102 46-47 1.475692577786198 1102 48-49 1.4130451601999745 1102 50-51 1.5220314074359393 1102 52-53 1.4405545179228305 1102 54-55 1.9195589708783523 1102 56-57 1.287527847111562 1102 58-59 1.4166611147610055 1102 60-61 1.3406127948030644 1102 62-63 1.6686906749857542 1102 64-65 1.8420440163059553 1102 66-67 1.9719916878042056 1102 68-69 1.8682832488993881 1102 70-71 1.4193512629116825 1102 72-73 1.5662128650750802 1102 74-75 1.8691000119490049 1102 76-77 1.6968674167953672 1102 78-79 1.5929162870648312 1102 80-81 1.599052970278322 1102 82-83 1.2619716590351366 1102 84-85 1.2920567824309686 1102 86-87 1.3710205524429249 1102 88-89 1.4300983361229775 1102 90-91 1.177143283104197 1102 92-93 1.200083608725592 1102 94-95 1.1985337582063913 1102 96-97 1.2936031735142386 1102 98-99 1.1403824036197214 1102 100-101 1.0562616016524409 1102 102-103 0.9418410936125312 1102 104-105 1.0912278988522957 1102 106-107 1.2877690987534116 1102 108-109 1.200032566784536 1102 110-111 1.0875940370975776 1102 112-113 1.0637415819139378 1102 114-115 1.2628694542775705 1102 116-117 1.0615333159540725 1102 118-119 0.5931512009173296 1102 120-121 0.9670875543163149 1102 122-123 1.334234660382851 1102 124-125 1.3615186554729988 1103 1 0.7119519075601453 1103 2 0.5242118107754834 1103 3 0.5345027825578441 1103 4 0.4994231153936397 1103 5 0.8941917369379837 1103 6 0.8930208861608726 1103 7 1.2149739820667484 1103 8 1.090066223512654 1103 9 1.7824896489373074 1103 10-11 1.4098865694446943 1103 12-13 1.407371417371138 1103 14-15 1.1076829594608952 1103 16-17 1.1242026984237796 1103 18-19 1.2616188815211231 1103 20-21 1.5044728762853907 1103 22-23 1.4420566949373281 1103 24-25 1.885161298336925 1103 26-27 1.273154200912856 1103 28-29 1.3584387276533505 1103 30-31 1.8941861862359453 1103 32-33 1.7865028023177132 1103 34-35 1.8133648625135876 1103 36-37 1.8444913929098021 1103 38-39 1.8292569211683478 1103 40-41 1.96883179557884 1103 42-43 1.946527002969411 1103 44-45 1.8866447860790458 1103 46-47 1.788962437058835 1103 48-49 1.6924175828098775 1103 50-51 1.8369391064017506 1103 52-53 1.6366734472208897 1103 54-55 1.9985371678369717 1103 56-57 1.7712210823486174 1103 58-59 1.9626783164304342 1103 60-61 1.9768676262436173 1103 62-63 2.0184128516087974 1103 64-65 2.206976587552603 1103 66-67 2.2371505968064795 1103 68-69 2.256677593937617 1103 70-71 2.06656805468592 1103 72-73 2.110192529498267 1103 74-75 2.083898770324744 1103 76-77 1.8647264606054037 1103 78-79 2.01916350758745 1103 80-81 1.959024516389043 1103 82-83 1.8638816014806814 1103 84-85 2.056734661521837 1103 86-87 1.9742999626713988 1103 88-89 1.9254536863993117 1103 90-91 1.9352969142084788 1103 92-93 1.6728034423797808 1103 94-95 1.5611367916297603 1103 96-97 1.4988007340409304 1103 98-99 1.7316517356000034 1103 100-101 1.769276768769295 1103 102-103 1.5940845733441122 1103 104-105 1.5108195457454983 1103 106-107 1.609174183642505 1103 108-109 1.5727869729090855 1103 110-111 1.647972991168423 1103 112-113 1.5561363846021372 1103 114-115 1.3578646539273684 1103 116-117 1.4715242624438503 1103 118-119 1.5306339768628483 1103 120-121 1.5344765120185144 1103 122-123 1.6235465438611882 1103 124-125 1.5084971707968151 1104 1 0.6835407405300415 1104 2 0.585809995598229 1104 3 0.6846591453495918 1104 4 0.5342422828860904 1104 5 0.8709118725218374 1104 6 0.8987586261261136 1104 7 1.2635934365205976 1104 8 1.0274829695471261 1104 9 1.6978325922352582 1104 10-11 1.2615694096013144 1104 12-13 1.4570358485831747 1104 14-15 1.465428646073434 1104 16-17 1.2193873242972018 1104 18-19 1.1314301576535684 1104 20-21 1.475179970960255 1104 22-23 1.4982630377520465 1104 24-25 1.6710049252081802 1104 26-27 1.549660109996431 1104 28-29 1.554749483568422 1104 30-31 1.974243591044317 1104 32-33 2.0049957820066027 1104 34-35 1.9668245625432377 1104 36-37 1.902108462179143 1104 38-39 2.023370516344162 1104 40-41 2.01294004997116 1104 42-43 2.0315573663415947 1104 44-45 1.7870588299276662 1104 46-47 1.81665706202228 1104 48-49 1.6233063565299624 1104 50-51 1.9522669435833429 1104 52-53 1.8075529505311572 1104 54-55 2.062935857481225 1104 56-57 1.9235405407649822 1104 58-59 2.0387124745780802 1104 60-61 2.030536940278715 1104 62-63 2.088243125894831 1104 64-65 2.235419587845012 1104 66-67 2.403728615864466 1104 68-69 2.4346882178679934 1104 70-71 2.113630694245213 1104 72-73 2.3559547509258962 1104 74-75 2.3758535221419805 1104 76-77 2.2787002212264724 1104 78-79 2.207895480289622 1104 80-81 2.089236224420155 1104 82-83 2.1096229139758726 1104 84-85 2.4498157000019454 1104 86-87 2.333152961286398 1104 88-89 2.2275223315991575 1104 90-91 2.2678903297585506 1104 92-93 1.9936293577407334 1104 94-95 1.877171820591375 1104 96-97 1.9804488778993985 1104 98-99 2.0775988327118924 1104 100-101 2.030375104809824 1104 102-103 2.0118089813981364 1104 104-105 1.9315020978756934 1104 106-107 2.060426456412138 1104 108-109 1.9746958488057231 1104 110-111 2.0223000093776804 1104 112-113 1.9884000968288191 1104 114-115 1.9420157066333843 1104 116-117 1.9432209291214804 1104 118-119 1.904792392332265 1104 120-121 1.8640946334691826 1104 122-123 2.054311821751863 1104 124-125 1.861544299217286 1105 1 0.38274293372260715 1105 2 0.5058580502834218 1105 3 0.33638780536347923 1105 4 0.5752176656671253 1105 5 0.7557429324797695 1105 6 0.8710116433967947 1105 7 1.1883627814984514 1105 8 1.1527255517830035 1105 9 1.7881754789982551 1105 10-11 1.3309979369028397 1105 12-13 1.4407287733285443 1105 14-15 1.3992945841910327 1105 16-17 1.5047247504791414 1105 18-19 1.6354959031280103 1105 20-21 1.7849562384379496 1105 22-23 1.7106956745387194 1105 24-25 1.9353445996797376 1105 26-27 1.689065423442429 1105 28-29 1.6194035491763614 1105 30-31 1.965779631468724 1105 32-33 1.909932194434326 1105 34-35 2.0783271517507345 1105 36-37 1.7804553621449912 1105 38-39 1.7972330983363953 1105 40-41 1.9390810441781205 1105 42-43 1.9633679724051731 1105 44-45 1.8509778490587578 1105 46-47 1.7848708212717597 1105 48-49 1.8934687906404584 1105 50-51 1.9518956476512912 1105 52-53 1.896783324959209 1105 54-55 2.110638267407179 1105 56-57 1.8664686461929065 1105 58-59 1.7668911949354182 1105 60-61 1.689051536476704 1105 62-63 1.5652523701732086 1105 64-65 1.9557179764725419 1105 66-67 2.155445053922925 1105 68-69 2.1678888257432547 1105 70-71 1.865432410899416 1105 72-73 2.142664180026962 1105 74-75 2.200953842388653 1105 76-77 1.9464519827010633 1105 78-79 2.131382738213361 1105 80-81 2.050659370917387 1105 82-83 2.032387425975987 1105 84-85 2.3420890269400374 1105 86-87 2.246373265766355 1105 88-89 2.059971937647415 1105 90-91 2.0814881786943964 1105 92-93 1.8509983126377882 1105 94-95 1.7578996439318502 1105 96-97 1.8150366187672198 1105 98-99 1.8594705289031594 1105 100-101 1.963857367844387 1105 102-103 1.7786992718601766 1105 104-105 1.3121545852401155 1105 106-107 1.539261291421635 1105 108-109 1.6132996220495421 1105 110-111 1.6214888409469523 1105 112-113 1.6722870009053352 1105 114-115 1.648853910343906 1105 116-117 1.6545221115072408 1105 118-119 1.6378853605422101 1105 120-121 1.6856890995564022 1105 122-123 1.7957089209434436 1105 124-125 1.7165856200373284 1106 1 0.3629822669184328 1106 2 0.21637695526907308 1106 3 0.34337942500563656 1106 4 0.22587314739562458 1106 5 0.38015291546629015 1106 6 0.5332618014698873 1106 7 0.8063858563947015 1106 8 0.3738763080102494 1106 9 1.130288734445564 1106 10-11 0.7916096589119803 1106 12-13 0.6414517962822259 1106 14-15 0.7280390486521142 1106 16-17 0.7542297064829597 1106 18-19 0.791888434685994 1106 20-21 0.674213467773292 1106 22-23 0.6001928252488398 1106 24-25 0.3910372380239977 1106 26-27 0.4938804630193232 1106 28-29 0.46499274353413256 1106 30-31 0.7787839899542597 1106 32-33 0.516676302013849 1106 34-35 0.6049881114918776 1106 36-37 0.5857170997925394 1106 38-39 0.7063139455207441 1106 40-41 0.9393392467906629 1106 42-43 0.9855696613530966 1106 44-45 0.8367509129266466 1106 46-47 0.9180986400697293 1106 48-49 0.7806556300568737 1106 50-51 1.068633591274338 1106 52-53 0.8082885424642079 1106 54-55 1.313058822512989 1106 56-57 1.1655114246866987 1106 58-59 1.1688883406778174 1106 60-61 1.2914898959184242 1106 62-63 1.379899615806675 1106 64-65 1.358234667043913 1106 66-67 1.4605653106868814 1106 68-69 1.422904144228248 1106 70-71 1.3582161307418303 1106 72-73 1.224851220139577 1106 74-75 1.5273637133032452 1106 76-77 1.1758748400505752 1106 78-79 1.2825931747335488 1106 80-81 1.4308932992255272 1106 82-83 1.2800807113170976 1106 84-85 1.4057979085614747 1106 86-87 1.4883992481742432 1106 88-89 1.17630018283538 1106 90-91 1.3492972240123962 1106 92-93 1.2275167383370764 1106 94-95 1.0754684300695878 1106 96-97 1.0582975782699044 1106 98-99 0.945201784375115 1106 100-101 0.7438653638988164 1106 102-103 0.6410454064315516 1106 104-105 0.8568237166945352 1106 106-107 0.8535101650390686 1106 108-109 0.9085057251215467 1106 110-111 0.844496891447676 1106 112-113 0.6522693237432478 1106 114-115 0.7159831320678975 1106 116-117 0.9723489015484184 1106 118-119 0.7276527556367149 1106 120-121 0.7172677122568523 1106 122-123 1.0006482331953315 1106 124-125 0.9948652045445314 1107 1 0.34688527011249093 1107 2 0.4478117852088488 1107 3 0.24652763922209076 1107 4 0.3085927748463817 1107 5 0.25894063365598896 1107 6 0.5338978505747143 1107 7 0.7853205941912655 1107 8 0.7612247525084435 1107 9 1.2728037228732632 1107 10-11 0.8502629660581746 1107 12-13 1.1003770844816927 1107 14-15 0.9504695801012133 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-4.302641869697219 2216 92-93 -3.775932183461652 2216 94-95 -3.6345553235725596 2216 96-97 -3.8379766102260255 2216 98-99 -3.6694807684251884 2216 100-101 -4.079761088434122 2216 102-103 -4.073600783934452 2216 104-105 -3.7810497262927854 2216 106-107 -3.951120457167832 2216 108-109 -4.000433924256438 2216 110-111 -3.599082305461991 2216 112-113 -3.7787734910554747 2216 114-115 -3.4831822634876985 2216 116-117 -3.3608411858316103 2216 118-119 -3.5714070157049598 2216 120-121 -3.557625994523402 2216 122-123 -3.7264021122477935 2216 124-125 -3.632945603756177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 113122.0 3 37757.0 4 18080.0 5 14715.0 6 15223.0 7 18001.0 8 19658.0 9 21006.0 10 22381.0 11 24050.0 12 26170.0 13 28234.0 14 29358.0 15 24148.0 16 17614.0 17 15385.0 18 13342.0 19 12394.0 20 12290.0 21 13328.0 22 15925.0 23 21470.0 24 29310.0 25 38961.0 26 48071.0 27 54504.0 28 61045.0 29 69735.0 30 77720.0 31 87200.0 32 101799.0 33 120989.0 34 146604.0 35 185434.0 36 256803.0 37 329225.0 38 121116.0 39 660.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.76452085695541 20.10585638692969 14.261192937067918 23.868429819046984 2 15.723059205249374 20.936546959262834 38.72423958922108 24.61615424626672 3 18.977014150882944 25.388551577296898 29.67593192055778 25.958502351262382 4 12.778125809357894 17.555313040263687 36.59867138021946 33.067889770158956 5 12.863997115113088 38.957772732957494 33.969941139998774 14.208289011930653 6 31.3858119828091 37.680289019256904 17.075469822014515 13.85842917591948 7 27.384070724473148 32.987748241459514 20.844921729419738 18.783259304647604 8 25.93782025758045 36.371185247480255 19.157496353013286 18.533498141926007 9 25.646414860703004 16.22947755175274 19.952873109610238 38.17123447793402 10-11 24.811772229040173 27.362840212001238 26.987224331441737 20.83816322751685 12-13 25.66208403065736 24.529380855809375 27.78757628212975 22.02095883140351 14-15 22.461341362256547 25.7662455547704 26.056436683623392 25.71597639934966 16-17 21.819923066860415 29.153673592770545 26.58393967678182 22.44246366358722 18-19 21.640533851864944 27.18894732190207 29.51663867774439 21.6538801484886 20-21 22.44208356636318 26.378175899436286 29.663603967624557 21.516136566575973 22-23 22.046183982437668 26.260502762620163 29.616326692954186 22.076986561987983 24-25 21.871566647368446 26.43181024691413 29.6898657816001 22.006757324117327 26-27 21.60160530434558 26.820656383155267 29.520337766216613 22.05740054628254 28-29 21.612856323378242 26.84142212039976 29.68414265397775 21.86157890224425 30-31 21.642027344289463 26.483215025548958 29.612749620218203 22.26200800994338 32-33 21.60530681545996 26.575485697972358 29.470869611715617 22.348337874852064 34-35 22.03230061803823 26.657069433733792 29.367687449868633 21.94294249835934 36-37 22.107927060472704 26.344613722961668 29.36407274861389 22.18338646795174 38-39 21.862368485306483 26.688055930272768 29.334093447659832 22.11548213676092 40-41 21.98132844263744 26.479383949089623 29.35449443167757 22.18479317659537 42-43 22.00751631488445 26.561232908979143 29.024076690452716 22.407174085683693 44-45 22.02119096015586 26.781124927054016 28.932879530688577 22.264804582101547 46-47 22.257912622034983 27.020522360439355 28.325705329777012 22.39585968774865 48-49 22.328993776369117 27.131017969999473 28.038776274103146 22.501211979528264 50-51 22.339209028208064 27.195991314337554 27.81151679227336 22.65328286518102 52-53 22.580591702587828 27.197340847241456 27.754564425192214 22.467503024978498 54-55 22.24019375624655 27.119767637653165 27.889477868424624 22.750560737675666 56-57 22.26267566016048 27.419999195694224 27.749499408039785 22.567825736105508 58-59 22.23886315657361 27.087930274828786 27.88388153402801 22.78932503456959 60-61 22.284381439677006 27.149424149526237 28.0650487200303 22.50114569076646 62-63 22.25273480838167 27.139604877722185 28.102850493753696 22.504809820142448 64-65 22.075277214220396 26.956042818962178 28.00893948425508 22.959740482562346 66-67 21.997784759053857 27.26943918146626 27.781884988757984 22.950891070721898 68-69 22.127440207230485 27.080231987482733 28.04105719719577 22.751270608091012 70-71 22.114353292436682 27.111774602623235 27.95810192520076 22.81577017973932 72-73 22.191079061860695 27.17311990490182 27.68268500231289 22.953116030924598 74-75 22.04581734228302 27.38268122846622 27.58714717305336 22.9843542561974 76-77 22.192143871691368 27.237278338196052 27.74759876506655 22.822979025046028 78-79 22.29214537819601 27.264322566517656 27.643454363981995 22.80007769130434 80-81 22.099902135583203 27.429988494467867 27.583866976868954 22.886242393079975 82-83 22.27538344802046 27.366899664710846 27.55496121437691 22.80275567289178 84-85 22.085802405574974 27.24006298316221 27.452407983464933 23.221726627797885 86-87 22.218993708301532 27.169911212675498 27.60852262859269 23.002572450430282 88-89 22.207368471758258 27.243445408195054 27.465890755845734 23.083295364200946 90-91 22.106599635322617 27.47838760627217 27.531131480956077 22.883881277449134 92-93 22.165612723856967 27.28680693998028 27.607254461671587 22.940325874491165 94-95 21.993037164218844 27.18553162057738 27.6155215331939 23.205909682009874 96-97 22.15598858219156 27.130751937549412 27.56037813646128 23.152881343797745 98-99 21.7908395118142 27.123792494963162 27.440520198848606 23.64484779437403 100-101 21.816817861921223 27.13467039213542 27.659042271696272 23.38946947424709 102-103 21.965399535619998 26.92582028829743 27.65206588998949 23.45671428609308 104-105 21.9008416032356 26.97282245548041 27.6283749863003 23.497960954983686 106-107 21.658099575066657 27.069475750560528 27.539397094473262 23.733027579899556 108-109 21.87199905251263 27.06605498343444 27.27607987707412 23.78586608697881 110-111 21.616744913271003 27.052827119381114 27.57142677216019 23.759001195187697 112-113 21.814523880547547 27.209859357219408 27.467767061085148 23.5078497011479 114-115 21.666767941365265 27.35174765143974 27.235233872740793 23.746250534454205 116-117 21.580041249286843 27.228838086163897 27.455457266507782 23.735663398041478 118-119 21.51527485367712 27.322958594734676 27.204256312080055 23.95751023950815 120-121 21.415449097191807 27.296109684886645 27.370111805293767 23.91832941262778 122-123 21.422397068428957 27.37865330513246 27.2585594750607 23.940390151377887 124-125 21.325686561003142 27.320482937293995 27.180978620681728 24.17285188102114 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 313.0 1 267.5 2 455.5 3 727.5 4 557.0 5 389.5 6 716.5 7 1732.0 8 2831.5 9 3235.5 10 2656.5 11 1791.5 12 1527.5 13 1584.5 14 1740.5 15 1929.0 16 2245.5 17 2556.0 18 2880.0 19 3237.0 20 3657.0 21 4296.0 22 5275.5 23 6608.5 24 8177.0 25 10299.5 26 13295.5 27 16798.5 28 20194.0 29 23747.5 30 27927.5 31 33164.5 32 38768.5 33 43238.0 34 47308.5 35 51353.0 36 56233.0 37 61338.0 38 64320.0 39 65660.5 40 67237.0 41 70325.0 42 72889.0 43 74743.5 44 76897.0 45 79199.0 46 80476.0 47 80720.0 48 82566.0 49 84002.5 50 82700.0 51 80034.0 52 77292.5 53 75927.5 54 74414.5 55 69019.0 56 62324.0 57 56793.0 58 52342.5 59 47090.5 60 39692.5 61 32524.0 62 26364.0 63 20257.0 64 14877.5 65 11284.0 66 8501.5 67 6613.0 68 5167.5 69 3811.0 70 2854.5 71 2167.5 72 1722.5 73 1477.5 74 1217.5 75 902.5 76 606.5 77 355.5 78 213.5 79 125.5 80 73.0 81 50.0 82 37.5 83 22.5 84 12.0 85 6.5 86 6.0 87 5.5 88 2.5 89 0.5 90 1.0 91 1.0 92 0.5 93 1.5 94 1.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10632717392889514 2 1.7677003146948484E-4 3 0.0 4 0.180835742193283 5 0.0 6 8.838501573474242E-5 7 0.03827071181314347 8 0.0 9 0.0 10-11 4.4192507867371215E-4 12-13 4.4192507867371215E-4 14-15 3.535400629389697E-4 16-17 0.0036679781529918103 18-19 0.0011931977124190229 20-21 0.002762031741710701 22-23 0.0013036789820874508 24-25 0.004286673263135007 26-27 0.0034691118675886402 28-29 0.002872513011379129 30-31 6.407913640768826E-4 32-33 4.419250786737121E-5 34-35 9.722351730821667E-4 36-37 9.280426652147955E-4 38-39 4.198288247400265E-4 40-41 0.0011931977124190229 42-43 7.070801258779394E-4 44-45 9.943314270158524E-4 46-47 1.1048126966842804E-4 48-49 0.0 50-51 0.04147466863352788 52-53 4.419250786737121E-5 54-55 0.13613502048543702 56-57 2.651550472042273E-4 58-59 0.0 60-61 0.0 62-63 3.7563631687265533E-4 64-65 0.06182531850645233 66-67 0.05400324461392762 68-69 0.0014362565056895646 70-71 0.0022096253933685606 72-73 0.07004512496978338 74-75 0.004551828310339235 76-77 0.00145835275962325 78-79 0.0011269089506179659 80-81 1.1048126966842804E-4 82-83 1.7677003146948484E-4 84-85 0.0 86-87 1.7677003146948484E-4 88-89 0.0010606201888169091 90-91 2.2096253933685608E-4 92-93 0.0023863954248380457 94-95 0.004397154532803436 96-97 0.002032855361899076 98-99 0.0 100-101 0.0010827164427505947 102-103 0.0011048126966842803 104-105 1.3257752360211364E-4 106-107 6.628876180105682E-5 108-109 0.0 110-111 1.1048126966842804E-4 112-113 8.838501573474242E-5 114-115 1.9886628540317046E-4 116-117 0.0 118-119 0.001016427680949538 120-121 0.0023863954248380457 122-123 5.745026022758257E-4 124-125 0.007645303861055219 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2262827.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.606215257044354 #Duplication Level Percentage of deduplicated Percentage of total 1 81.35881427585828 50.935674396122764 2 10.739241414211486 13.446865193509797 3 3.4474347680881445 6.474925295266354 4 1.4935542577602168 3.7402311743764494 5 0.8015091333487058 2.508972666645807 6 0.46667731060014966 1.7530140097806912 7 0.3077799942703058 1.3488258401169047 8 0.21758481139201624 1.0897729230937574 9 0.1596491367833525 0.8995525400753897 >10 0.9118015350234152 10.458669778678944 >50 0.06511095964112455 2.7529200585205325 >100 0.02925901307402826 3.2054138840173447 >500 0.0012235285967709855 0.49470401843514555 >1k 2.1591681119483897E-4 0.3081142251624923 >5k 1.439445407965593E-4 0.582343996197726 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6722 0.2970620378844693 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6202 0.27408193379343626 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4024 0.17783065165830175 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.419250786737121E-5 2 0.0 0.0 0.0 0.0 4.419250786737121E-5 3 0.0 0.0 0.0 0.0 4.419250786737121E-5 4 0.0 0.0 0.0 4.419250786737121E-5 4.419250786737121E-5 5 0.0 0.0 0.0 4.419250786737121E-5 4.419250786737121E-5 6 0.0 0.0 0.0 4.419250786737121E-5 8.838501573474242E-5 7 0.0 0.0 0.0 4.419250786737121E-5 8.838501573474242E-5 8 0.0 0.0 0.0 8.838501573474242E-5 1.3257752360211364E-4 9 0.0 0.0 0.0 8.838501573474242E-5 1.3257752360211364E-4 10-11 0.0 0.0 0.0 8.838501573474242E-5 1.3257752360211364E-4 12-13 0.0 0.0 0.0 8.838501573474242E-5 2.651550472042273E-4 14-15 0.0 0.0 0.0 1.5467377753579923E-4 3.314438090052841E-4 16-17 0.0 0.0 0.0 1.7677003146948484E-4 4.4192507867371215E-4 18-19 2.2096253933685606E-5 0.0 0.0 1.9886628540317046E-4 4.8611758654108333E-4 20-21 4.419250786737121E-5 4.419250786737121E-5 0.0 3.093475550715985E-4 5.524063483421401E-4 22-23 4.419250786737121E-5 4.419250786737121E-5 0.0 5.965988562095113E-4 6.407913640768826E-4 24-25 4.419250786737121E-5 4.419250786737121E-5 0.0 8.617539034137386E-4 6.628876180105682E-4 26-27 4.419250786737121E-5 4.419250786737121E-5 0.0 0.0011048126966842803 6.849838719442538E-4 28-29 6.628876180105682E-5 4.419250786737121E-5 0.0 0.0016572190450264204 7.070801258779394E-4 30-31 8.838501573474242E-5 6.628876180105682E-5 0.0 0.003645881899058125 7.070801258779394E-4 32-33 8.838501573474242E-5 8.838501573474242E-5 0.0 0.007689496368922591 7.291763798116251E-4 34-35 8.838501573474242E-5 8.838501573474242E-5 0.0 0.013699677438885074 7.954651416126818E-4 36-37 8.838501573474242E-5 8.838501573474242E-5 0.0 0.023665087962977283 7.954651416126818E-4 38-39 8.838501573474242E-5 8.838501573474242E-5 0.0 0.04222594126727319 8.838501573474243E-4 40-41 8.838501573474242E-5 8.838501573474242E-5 0.0 0.06454315774029566 8.838501573474243E-4 42-43 8.838501573474242E-5 1.1048126966842802E-4 0.0 0.08666150792791495 8.838501573474243E-4 44-45 8.838501573474242E-5 1.3257752360211364E-4 0.0 0.11167446738084705 9.280426652147955E-4 46-47 8.838501573474242E-5 1.3257752360211364E-4 0.0 0.1418579502542616 0.0010385239348832235 48-49 8.838501573474242E-5 1.3257752360211364E-4 0.0 0.17767597788076597 0.0011490052045516515 50-51 8.838501573474242E-5 1.3257752360211364E-4 0.0 0.21974724537050336 0.001237390220286394 52-53 8.838501573474242E-5 1.3257752360211364E-4 0.0 0.2606474114017554 0.0015467377753579925 54-55 8.838501573474242E-5 1.3257752360211364E-4 0.0 0.3045084754601214 0.0015467377753579925 56-57 8.838501573474242E-5 1.3257752360211364E-4 0.0 0.35734061861556365 0.0015909302832253637 58-59 8.838501573474242E-5 1.3257752360211364E-4 0.0 0.4167574454432442 0.0016351227910927349 60-61 8.838501573474242E-5 1.3257752360211364E-4 0.0 0.4891447733299983 0.0016572190450264204 62-63 8.838501573474242E-5 1.3257752360211364E-4 0.0 0.5604051922661344 0.0018118928225622198 64-65 8.838501573474242E-5 1.3257752360211364E-4 0.0 0.6318644774876736 0.0018339890764959054 66-67 8.838501573474242E-5 1.3257752360211364E-4 0.0 0.7164489375458221 0.0018781815843632766 68-69 1.3257752360211364E-4 1.3257752360211364E-4 0.0 0.8155506364384021 0.0019002778382969621 70-71 1.3257752360211364E-4 1.3257752360211364E-4 0.0 0.9293242479429493 0.0019002778382969621 72-73 1.3257752360211364E-4 1.3257752360211364E-4 0.0 1.0458598911892072 0.0019223740922306477 74-75 1.3257752360211364E-4 1.3257752360211364E-4 0.0 1.1623955344354648 0.001966566600098019 76-77 1.3257752360211364E-4 1.3257752360211364E-4 0.0 1.2933600315004194 0.0019886628540317047 78-79 1.3257752360211364E-4 1.3257752360211364E-4 0.0 1.4501108569059853 0.002032855361899076 80-81 1.7677003146948484E-4 1.7677003146948484E-4 0.0 1.6313664279240083 0.002077047869766447 82-83 1.7677003146948484E-4 1.7677003146948484E-4 0.0 1.8254157299696354 0.002342202916970674 84-85 1.7677003146948484E-4 1.7677003146948484E-4 0.0 2.021299021091758 0.002342202916970674 86-87 1.7677003146948484E-4 1.7677003146948484E-4 0.0 2.2290921930841376 0.002408491678771731 88-89 1.7677003146948484E-4 1.7677003146948484E-4 0.0 2.4603957792619586 0.002474780440572788 90-91 1.9886628540317046E-4 2.651550472042273E-4 0.0 2.7311190824574747 0.002474780440572788 92-93 2.2096253933685608E-4 2.651550472042273E-4 0.0 3.0202485651797506 0.0025189729484401593 94-95 2.2096253933685608E-4 2.651550472042273E-4 0.0 3.3078534063806027 0.0025189729484401593 96-97 2.2096253933685608E-4 2.8725130113791287E-4 0.0 3.6037664390605206 0.0027841279956443863 98-99 2.2096253933685608E-4 3.093475550715985E-4 0.0 3.9195219077728876 0.0029167055192465 100-101 2.2096253933685608E-4 3.093475550715985E-4 0.0 4.295887401025354 0.0029167055192465 102-103 2.2096253933685608E-4 3.093475550715985E-4 0.0 4.703077168515312 0.002960898027113871 104-105 2.8725130113791287E-4 3.093475550715985E-4 0.0 5.109427278355792 0.0030050905349812427 106-107 3.093475550715985E-4 3.093475550715985E-4 0.0 5.515247078101861 0.0030050905349812427 108-109 3.093475550715985E-4 3.093475550715985E-4 0.0 5.9396277311522265 0.0030271867889149285 110-111 3.093475550715985E-4 3.093475550715985E-4 0.0 6.41566942589955 0.003071379296782299 112-113 3.093475550715985E-4 3.7563631687265533E-4 0.0 6.934334794484952 0.003137668058583356 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4680 0.0 62.865753 1 GTATCAA 8545 0.0 51.75105 1 ATCAACG 10790 0.0 38.724003 3 TATCAAC 11425 0.0 38.44988 2 TCAACGC 10950 0.0 37.886 4 CAACGCA 10890 0.0 37.802128 5 AACGCAG 11130 0.0 36.612843 6 GTACATG 9790 0.0 31.881008 1 TACATGG 9755 0.0 31.039886 2 CGCAGAG 13350 0.0 30.60204 8 ACGCAGA 13255 0.0 30.518785 7 GTGGTAT 2530 0.0 28.541573 1 ACATGGG 9705 0.0 28.313208 3 GCAGAGT 14465 0.0 27.544268 9 GAGTACT 8915 0.0 26.583996 12-13 CCGCGTA 70 1.0599644E-4 25.488043 10-11 TGGTATC 2600 0.0 25.446806 2 AGAGTAC 13780 0.0 24.75127 10-11 AGTACTT 9760 0.0 24.373812 12-13 GTACTTT 10045 0.0 24.304197 14-15 >>END_MODULE