FastQCFastQC Report
Thu 26 May 2016
SRR1364495_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364495_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802162
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52980.18906829797848945No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49110.1752575332903665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35760.12761574812591134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97550.054.0035361
GGTATCA51100.052.5365831
ATCAACG120050.041.582643
TCAACGC121150.040.771474
CAACGCA123150.040.5425955
TATCAAC132750.038.9484142
AACGCAG122800.038.844236
GTACATG143000.038.2964021
TACATGG149750.034.1693042
CGCAGAG136450.033.4332548
ACGCAGA140150.033.2724157
ACATGGG148400.030.7520263
GCAGAGT145600.029.041599
CATGGGA105050.025.6370264
GAGTACT67150.025.42125512-13
CGCATAG4550.024.869428
AGAGTAC138000.024.36807610-11
CGCGATA2850.023.995016104-105
CAGAGTA148000.023.70685610-11
ACGCATA4050.023.5277617
Produced by FastQC (version 0.11.5)