##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364495_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2802162 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.840410368850907 30.0 24.0 31.0 2.0 34.0 2 25.116153884036684 30.0 25.0 31.0 2.0 34.0 3 25.10742954904106 30.0 24.0 31.0 2.0 34.0 4 27.733209928619402 33.0 25.0 35.0 2.0 37.0 5 28.33375800542581 35.0 27.0 35.0 2.0 37.0 6 28.451594875671 35.0 27.0 37.0 2.0 37.0 7 28.23953218978774 35.0 26.0 37.0 2.0 37.0 8 27.858081724040222 35.0 25.0 37.0 2.0 37.0 9 28.28899542567489 35.0 23.0 39.0 2.0 39.0 10-11 28.403010068654133 34.5 24.0 39.0 2.0 39.0 12-13 28.22956952524515 35.0 23.0 39.0 2.0 39.0 14-15 28.77757460132569 34.5 23.0 39.5 2.0 41.0 16-17 29.030199895652 35.5 25.0 40.0 2.0 41.0 18-19 29.01372761460615 35.5 24.5 40.0 2.0 41.0 20-21 28.794467985790973 35.0 23.0 40.0 2.0 41.0 22-23 28.838380150755025 35.0 24.0 40.0 2.0 41.0 24-25 28.86041313814119 35.0 24.0 40.0 2.0 41.0 26-27 28.94263572198895 35.0 24.5 40.0 2.0 41.0 28-29 28.864207886624683 35.0 24.5 40.0 2.0 41.0 30-31 28.49925950034295 35.0 23.5 39.5 2.0 41.0 32-33 28.46974032907448 35.0 24.0 40.0 2.0 41.0 34-35 28.17261671523631 34.5 22.5 39.0 2.0 41.0 36-37 27.91559356668173 34.0 20.5 39.0 2.0 41.0 38-39 27.752970206576208 34.0 19.5 39.0 2.0 41.0 40-41 27.771666841531648 34.0 20.0 39.0 2.0 41.0 42-43 27.619217054545743 34.0 19.0 39.0 2.0 41.0 44-45 27.25296877910699 33.5 16.5 39.0 2.0 41.0 46-47 26.98296636668401 33.0 15.5 39.0 2.0 40.0 48-49 26.697772291537746 33.0 14.0 39.0 2.0 40.0 50-51 26.67508034867363 33.0 13.5 39.0 2.0 40.0 52-53 26.483897255048067 33.0 9.5 39.0 2.0 40.0 54-55 26.360552137956333 33.0 9.0 38.5 2.0 40.0 56-57 25.960612377157354 33.0 8.0 38.0 2.0 40.0 58-59 25.740537484984806 32.0 7.5 38.0 2.0 40.0 60-61 25.343712105153095 32.0 7.0 38.0 2.0 40.0 62-63 25.290178619223298 32.0 4.0 38.0 2.0 40.0 64-65 25.12543064961983 32.0 2.0 37.0 2.0 40.0 66-67 24.895764234901478 31.5 2.0 37.0 2.0 40.0 68-69 24.72858921075941 31.5 2.0 36.5 2.0 39.5 70-71 24.58122406912948 31.0 2.0 36.0 2.0 39.0 72-73 24.553107029500794 32.0 2.0 36.0 2.0 39.0 74-75 24.281004452990224 31.0 2.0 35.5 2.0 39.0 76-77 24.0054507912105 31.0 2.0 35.0 2.0 38.5 78-79 23.705858190925433 31.0 2.0 35.0 2.0 37.0 80-81 23.69539805336023 31.0 2.0 35.0 2.0 37.0 82-83 23.49669540875938 31.0 2.0 35.0 2.0 36.5 84-85 23.30003333140625 31.0 2.0 35.0 2.0 36.0 86-87 23.091320380477647 31.0 2.0 35.0 2.0 36.0 88-89 22.859987038579497 31.0 2.0 35.0 2.0 35.5 90-91 22.684546253928218 30.5 2.0 35.0 2.0 35.0 92-93 22.57064991246045 30.0 2.0 35.0 2.0 35.0 94-95 22.49532700107988 30.0 2.0 34.0 2.0 35.0 96-97 22.357077677878724 30.0 2.0 34.0 2.0 35.0 98-99 22.26003385956986 30.0 2.0 34.0 2.0 35.0 100-101 22.19248762205754 30.0 2.0 34.0 2.0 35.0 102-103 21.150310545928463 28.5 2.0 33.5 2.0 34.5 104-105 21.845236463844703 30.0 2.0 34.0 2.0 35.0 106-107 21.915679393268483 30.0 2.0 34.0 2.0 35.0 108-109 21.852149518835812 30.0 2.0 34.0 2.0 35.0 110-111 21.73839485368797 30.0 2.0 34.0 2.0 35.0 112-113 21.70823278597026 30.0 2.0 34.0 2.0 35.0 114-115 21.649706726449075 30.0 2.0 34.0 2.0 35.0 116-117 21.449408528129354 30.0 2.0 34.0 2.0 35.0 118-119 21.298372292536975 30.0 2.0 34.0 2.0 35.0 120-121 21.137069163024833 30.0 2.0 34.0 2.0 35.0 122-123 20.86490627593979 30.0 2.0 34.0 2.0 35.0 124-125 19.953304626927352 27.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.7155236558324027 1101 2 1.297942785412605 1101 3 1.646457906108381 1101 4 1.9779957309699796 1101 5 1.5188101595169101 1101 6 1.5063897931501344 1101 7 1.4435586110230574 1101 8 1.7807662701847278 1101 9 2.1653084025587397 1101 10-11 2.121874611817862 1101 12-13 2.052647281835288 1101 14-15 2.3064824461772098 1101 16-17 1.8662183921621676 1101 18-19 1.8059835969364002 1101 20-21 2.2550160900168557 1101 22-23 2.111001478003928 1101 24-25 1.965848598323582 1101 26-27 1.6449656553995347 1101 28-29 1.5530739354736838 1101 30-31 1.6927800760267644 1101 32-33 1.9539933390576039 1101 34-35 2.243908855181779 1101 36-37 2.162145432377983 1101 38-39 2.2243368727616577 1101 40-41 2.057407882299252 1101 42-43 2.009054509656039 1101 44-45 2.111108465275443 1101 46-47 2.026830463256747 1101 48-49 2.121560665424415 1101 50-51 2.005712924084669 1101 52-53 1.8743003305029795 1101 54-55 1.8505739038672004 1101 56-57 2.161650835673374 1101 58-59 2.0164080066256496 1101 60-61 2.1771512601998673 1101 62-63 1.937433209365711 1101 64-65 1.8639266234357308 1101 66-67 2.0473958579165092 1101 68-69 1.819755702967175 1101 70-71 1.8288921467786956 1101 72-73 1.7671923952150799 1101 74-75 1.719211347494646 1101 76-77 1.8464221550017648 1101 78-79 1.871848660289043 1101 80-81 1.6702262443472122 1101 82-83 1.58873724504814 1101 84-85 1.5047465839704834 1101 86-87 1.4754356247279183 1101 88-89 1.6110375934140393 1101 90-91 1.734201784733287 1101 92-93 1.6392606629105693 1101 94-95 1.628599260572141 1101 96-97 1.6461645349893814 1101 98-99 1.4481625957594453 1101 100-101 1.417821695633318 1101 102-103 1.4283939805724906 1101 104-105 1.4342462034566879 1101 106-107 1.5627010558468974 1101 108-109 1.599555140840394 1101 110-111 1.7292250624256518 1101 112-113 1.5226830842025088 1101 114-115 1.5321811502672489 1101 116-117 1.6223635785004227 1101 118-119 1.6576795392280381 1101 120-121 1.6722458190241838 1101 122-123 1.8011094923329196 1101 124-125 1.7755075103084579 1102 1 0.8819697929001187 1102 2 0.6452979991266687 1102 3 0.7809156259093584 1102 4 1.080856198807922 1102 5 1.1420628484868551 1102 6 1.200325840485167 1102 7 1.0873091039549436 1102 8 1.0799304485170964 1102 9 1.3949055807410033 1102 10-11 1.182253944636141 1102 12-13 1.4550342594440941 1102 14-15 1.6342366739426915 1102 16-17 1.2407489937977267 1102 18-19 1.3618623026181105 1102 20-21 1.4096181869172995 1102 22-23 1.3902299537355667 1102 24-25 1.1555818977912544 1102 26-27 1.1532172821807691 1102 28-29 0.9707810771132586 1102 30-31 0.8401367632361492 1102 32-33 0.8039028410034632 1102 34-35 1.196458065685107 1102 36-37 1.4728478210940992 1102 38-39 1.5336345116073211 1102 40-41 0.964399479771707 1102 42-43 0.9186003423568678 1102 44-45 1.282421907199378 1102 46-47 1.1165520171646222 1102 48-49 1.2124634537439825 1102 50-51 1.0146779770160137 1102 52-53 1.2383914298280096 1102 54-55 0.9573713073776382 1102 56-57 1.1522142642404418 1102 58-59 1.2114344934990307 1102 60-61 1.562811256773724 1102 62-63 1.2759839705373501 1102 64-65 1.4496675138424493 1102 66-67 1.380248432483853 1102 68-69 1.2625013993007776 1102 70-71 1.3707022583911979 1102 72-73 1.3108900119265066 1102 74-75 1.464878158189297 1102 76-77 1.3011463081977865 1102 78-79 1.223191851977429 1102 80-81 0.847051353809892 1102 82-83 0.8548842095629858 1102 84-85 0.856144422193843 1102 86-87 1.028886137334677 1102 88-89 0.8084735101486231 1102 90-91 0.6985522086335045 1102 92-93 0.6591531677169193 1102 94-95 0.9677566459850908 1102 96-97 1.0324579225360395 1102 98-99 1.213069227039611 1102 100-101 1.1117612941980646 1102 102-103 0.8563794940993894 1102 104-105 0.7543754639782918 1102 106-107 1.0409017526935713 1102 108-109 1.143406414491583 1102 110-111 0.9281075487359267 1102 112-113 0.7758549153819239 1102 114-115 0.8839154542559058 1102 116-117 1.0759329989990931 1102 118-119 1.0970289878651727 1102 120-121 1.1522114770426732 1102 122-123 1.2791716382170115 1102 124-125 1.1359477334656134 1103 1 1.477786176264356 1103 2 1.253317292021066 1103 3 1.3790745072872426 1103 4 1.8710075163388744 1103 5 1.4173956148094042 1103 6 1.3211349225707671 1103 7 1.2598617655620963 1103 8 1.5181649427270258 1103 9 2.2194153161708634 1103 10-11 1.8076736746885906 1103 12-13 2.003060062066659 1103 14-15 2.1940085621291914 1103 16-17 2.085059615294103 1103 18-19 1.6364831008043623 1103 20-21 2.136659164021726 1103 22-23 2.0209222004079024 1103 24-25 2.1186790884387 1103 26-27 1.9024506212183923 1103 28-29 1.7886858526301879 1103 30-31 1.9904877694025487 1103 32-33 1.869325128372818 1103 34-35 1.7287646480308183 1103 36-37 2.134676789790362 1103 38-39 2.1648047329872178 1103 40-41 2.148661159734502 1103 42-43 2.172673943091116 1103 44-45 1.9919322312180512 1103 46-47 2.246794276473995 1103 48-49 2.1389165577434603 1103 50-51 2.0268506559411392 1103 52-53 2.0048271581730184 1103 54-55 1.9861808069233255 1103 56-57 2.198031737188323 1103 58-59 2.3877945556010296 1103 60-61 2.307544615359763 1103 62-63 2.106569523470071 1103 64-65 2.024341701627275 1103 66-67 2.100744109720978 1103 68-69 2.0446823590616 1103 70-71 2.061771379227519 1103 72-73 1.9837137341707027 1103 74-75 1.9927975640371969 1103 76-77 2.0885156089008703 1103 78-79 2.2112989930872686 1103 80-81 1.6520259227139888 1103 82-83 1.8207441356806662 1103 84-85 1.7647598134814864 1103 86-87 1.8137802671921115 1103 88-89 1.8543848261584301 1103 90-91 1.9034324430948146 1103 92-93 1.8127835535983081 1103 94-95 1.6141147019713138 1103 96-97 1.761553245508928 1103 98-99 1.7765634854679142 1103 100-101 1.5475088137395367 1103 102-103 1.611771811509204 1103 104-105 1.7297776859170781 1103 106-107 1.5357309305471674 1103 108-109 1.8572711206768098 1103 110-111 1.907996772262301 1103 112-113 1.7258987871469031 1103 114-115 1.7162569460070074 1103 116-117 1.8607060996144504 1103 118-119 1.9345934842735026 1103 120-121 1.9117199637242202 1103 122-123 1.901639718223219 1103 124-125 1.7599368508750892 1104 1 1.1187913245523653 1104 2 0.9198335000765674 1104 3 0.9824528934385093 1104 4 1.2891268503006827 1104 5 1.048916421388654 1104 6 0.9304069269155981 1104 7 0.8912184905170975 1104 8 1.2169804066027332 1104 9 1.7068288630161703 1104 10-11 1.5838749671189767 1104 12-13 1.1009984763600436 1104 14-15 1.3591922881383098 1104 16-17 1.0918226844560195 1104 18-19 0.4747423643018891 1104 20-21 -3.9318376484959465 1104 22-23 -0.8772745364869117 1104 24-25 0.5917690965943443 1104 26-27 0.6942243799045755 1104 28-29 0.31337150917162404 1104 30-31 1.0424463954036156 1104 32-33 0.9030081795886886 1104 34-35 1.217031146861963 1104 36-37 1.3903531966086504 1104 38-39 1.33139601898149 1104 40-41 1.367861646287981 1104 42-43 1.229225038169453 1104 44-45 1.3365850774893104 1104 46-47 1.3969798219121934 1104 48-49 1.3557035004789704 1104 50-51 1.2505140415807858 1104 52-53 1.2319404961926672 1104 54-55 1.2178863112866267 1104 56-57 1.507683430044473 1104 58-59 1.39314991341098 1104 60-61 1.6176479867830444 1104 62-63 1.1832141087461991 1104 64-65 1.349396329909741 1104 66-67 1.3509134891836467 1104 68-69 1.2457979930130527 1104 70-71 1.4659331794511807 1104 72-73 1.3508348645538355 1104 74-75 1.2815538253253784 1104 76-77 1.3468554661438006 1104 78-79 1.295226592524667 1104 80-81 1.1784718239126306 1104 82-83 1.1819775511759048 1104 84-85 1.0807811731550707 1104 86-87 1.0192766080507987 1104 88-89 1.1577202846818047 1104 90-91 1.1279021597421952 1104 92-93 0.8976125424014008 1104 94-95 1.02923001018139 1104 96-97 1.1131076615751212 1104 98-99 0.9649886772648024 1104 100-101 0.5760927711582333 1104 102-103 1.0460192792938052 1104 104-105 1.1974962913806948 1104 106-107 1.23784482862402 1104 108-109 1.2329703596650212 1104 110-111 1.2965024003831083 1104 112-113 1.2531794763139423 1104 114-115 1.1950183658761446 1104 116-117 1.2546527591608232 1104 118-119 1.2099845101590425 1104 120-121 1.1908311317492526 1104 122-123 1.0922862179867785 1104 124-125 1.2148346873589695 1105 1 1.1827639790405158 1105 2 0.9711717977866243 1105 3 1.081543138071897 1105 4 1.5784113998943035 1105 5 1.1927401085404306 1105 6 1.1683880123159724 1105 7 0.9556097477848411 1105 8 1.0777106011959674 1105 9 1.5529623126213785 1105 10-11 1.3197330097275746 1105 12-13 1.4086401391679964 1105 14-15 1.6763576146154442 1105 16-17 1.480009647952297 1105 18-19 1.090508854819067 1105 20-21 1.1751513963630167 1105 22-23 1.2587574034456956 1105 24-25 1.2464569372346084 1105 26-27 1.1495430375169313 1105 28-29 0.7713876623141793 1105 30-31 1.2099869028799084 1105 32-33 1.08605470695732 1105 34-35 0.8667741295599143 1105 36-37 1.2057546258638823 1105 38-39 1.0711522624389431 1105 40-41 1.1234422159120712 1105 42-43 1.4386849805259914 1105 44-45 1.5878587530199049 1105 46-47 1.784811093161391 1105 48-49 1.316032629166596 1105 50-51 1.3685903546091822 1105 52-53 1.4126228487522994 1105 54-55 1.377298447644229 1105 56-57 1.6922253963244316 1105 58-59 1.407175809127839 1105 60-61 1.7386190851739123 1105 62-63 1.6532524796667012 1105 64-65 1.2699533899128745 1105 66-67 1.510424570746327 1105 68-69 1.1841485238938674 1105 70-71 1.1669916378999012 1105 72-73 1.0993849194166252 1105 74-75 1.3572936021386255 1105 76-77 1.1762517112108029 1105 78-79 1.1110742511417904 1105 80-81 1.0800246480400055 1105 82-83 1.1832674423091554 1105 84-85 1.140005358666368 1105 86-87 1.1379437395647436 1105 88-89 1.2458899215063575 1105 90-91 1.1846895005214968 1105 92-93 1.2155700310970268 1105 94-95 1.1345113118498595 1105 96-97 1.2271656581794517 1105 98-99 1.0853820197389332 1105 100-101 1.2333024382999618 1105 102-103 1.243103864081288 1105 104-105 1.2797078953860677 1105 106-107 1.2547584529133111 1105 108-109 1.3309881223562847 1105 110-111 1.2155942005131912 1105 112-113 1.261175420010968 1105 114-115 1.1880346961891703 1105 116-117 1.3189836583520282 1105 118-119 1.3977566845452358 1105 120-121 1.3338791725741324 1105 122-123 1.4208605722304348 1105 124-125 1.111913356454373 1106 1 -0.4680596345358907 1106 2 -0.3132586397319379 1106 3 -0.32230179169032525 1106 4 -0.532645169075689 1106 5 -1.1801970516588796 1106 6 -0.5378169897790386 1106 7 -0.5321938257181635 1106 8 -0.17420512552552836 1106 9 -0.12067127392167265 1106 10-11 -2.787761913153446 1106 12-13 -0.8726032612414407 1106 14-15 -0.5149395030767536 1106 16-17 -0.7750127841422305 1106 18-19 -3.3277995933005258 1106 20-21 -5.715560967592825 1106 22-23 -6.554989698864706 1106 24-25 -6.364643130923486 1106 26-27 -5.417763565297115 1106 28-29 -2.2345335146027168 1106 30-31 -3.9369195615541024 1106 32-33 -4.303684021031401 1106 34-35 -3.8255708255917327 1106 36-37 -6.138371078258473 1106 38-39 -6.538552613321961 1106 40-41 -2.948031522276864 1106 42-43 -1.725575591002766 1106 44-45 -0.9651760803534266 1106 46-47 -0.6740403096274044 1106 48-49 -0.48053919036842885 1106 50-51 -0.6558667893163737 1106 52-53 -0.7768288101376406 1106 54-55 -0.6753280604085106 1106 56-57 -0.5347839673164181 1106 58-59 -0.3769290613610572 1106 60-61 -0.1584820400425464 1106 62-63 -0.43758955937907373 1106 64-65 -0.5534471094363447 1106 66-67 -0.8950808121696454 1106 68-69 -0.7235838233284504 1106 70-71 -0.5303756216328601 1106 72-73 -0.49959509486479803 1106 74-75 -0.5305717233533045 1106 76-77 -0.6502426710848894 1106 78-79 -0.5883995134071931 1106 80-81 -0.9158342701211382 1106 82-83 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112-113 0.8835229505841866 2212 114-115 0.8528558692672092 2212 116-117 0.8586495092408803 2212 118-119 0.8119119824425063 2212 120-121 0.9471649737045418 2212 122-123 0.9885466844203314 2212 124-125 1.0554842401050237 2213 1 -1.8663884728779934 2213 2 -0.693880234592811 2213 3 -0.4259269440719713 2213 4 -0.7493877934081823 2213 5 -0.7226099206071375 2213 6 -1.4679690825056149 2213 7 -1.1363154217712506 2213 8 -1.4075085892062518 2213 9 -0.05296768064964397 2213 10-11 -0.801745309089938 2213 12-13 -0.924960959015177 2213 14-15 -0.6308292569166909 2213 16-17 -0.5690359178947695 2213 18-19 -0.4921174513871911 2213 20-21 0.06319001046792394 2213 22-23 -0.012693641684215606 2213 24-25 -0.1507792508013317 2213 26-27 -0.15444434762801507 2213 28-29 -0.1968697479287549 2213 30-31 -0.20247887633754047 2213 32-33 -0.23295519322137892 2213 34-35 0.0467270747034263 2213 36-37 0.05845005372847467 2213 38-39 -0.2532305991715269 2213 40-41 0.11461159750494332 2213 42-43 -0.0048509156545080145 2213 44-45 0.16709441217216892 2213 46-47 -0.09529446570385502 2213 48-49 0.13192471910760162 2213 50-51 -0.005386394896937929 2213 52-53 -0.20129791523875795 2213 54-55 -2.037702490294791 2213 56-57 -1.2077581735024552 2213 58-59 -0.210489465271543 2213 60-61 0.3310583222662089 2213 62-63 -0.43419341894134433 2213 64-65 -0.24119459247885544 2213 66-67 -0.029346617106220663 2213 68-69 -0.21323767442092034 2213 70-71 -0.13009999659351124 2213 72-73 -0.0540739218504136 2213 74-75 -0.01766403569818209 2213 76-77 -0.07109340167717804 2213 78-79 -0.3205178386293177 2213 80-81 -0.6294415702148548 2213 82-83 -0.5002340804300935 2213 84-85 -0.25143971803325726 2213 86-87 -0.08886390678197742 2213 88-89 -0.10252161855153474 2213 90-91 -0.01974677614143161 2213 92-93 -0.06872815515777475 2213 94-95 -0.13245603997701494 2213 96-97 -0.18994604231248502 2213 98-99 -0.1790485493377041 2213 100-101 -0.10588804187385037 2213 102-103 -0.07436500778644728 2213 104-105 -0.07491492970224556 2213 106-107 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0.2705019417434045 2214 38-39 0.4361880147687458 2214 40-41 0.3131598027292881 2214 42-43 0.2990775317214869 2214 44-45 0.4591315335308863 2214 46-47 0.6355033133049801 2214 48-49 0.5782972532074844 2214 50-51 0.3882801062154613 2214 52-53 0.3203007075186761 2214 54-55 0.25048922727616585 2214 56-57 0.39116620640682953 2214 58-59 0.23943084696993466 2214 60-61 0.44666275948250345 2214 62-63 0.2764585224953784 2214 64-65 0.1470813149412713 2214 66-67 0.242887841116314 2214 68-69 0.15928629512695025 2214 70-71 0.021716759117015982 2214 72-73 -0.045079870894390695 2214 74-75 0.1641710315307776 2214 76-77 0.16153115528417317 2214 78-79 -2.689445866966498 2214 80-81 -0.8566900377067199 2214 82-83 -0.20508925966181835 2214 84-85 -2.2981297915493037 2214 86-87 -0.7553282433113218 2214 88-89 -0.24083466633417316 2214 90-91 -1.9174407253768528 2214 92-93 -1.3916780737606835 2214 94-95 -0.4055237494595936 2214 96-97 -0.1964866926717761 2214 98-99 -0.046658528423972 2214 100-101 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32-33 -1.578019269164244 2215 34-35 -1.1818473861379601 2215 36-37 -1.1734592962938777 2215 38-39 -1.3115962599979056 2215 40-41 -1.6344831113794953 2215 42-43 -1.5552793956829483 2215 44-45 -1.257138900593084 2215 46-47 -1.612900472389729 2215 48-49 -1.6594059222300146 2215 50-51 -2.4817277547706453 2215 52-53 -2.0524825156891886 2215 54-55 -1.9704318229366216 2215 56-57 -1.7278053369065418 2215 58-59 -1.4985154852086033 2215 60-61 -1.415300650841548 2215 62-63 -1.6024257149552135 2215 64-65 -1.4606713684030659 2215 66-67 -1.6494541417943225 2215 68-69 -1.6993367413531573 2215 70-71 -1.5045441689155474 2215 72-73 -1.5706550527777097 2215 74-75 -1.6833169581100478 2215 76-77 -1.6505656238509907 2215 78-79 -3.737728565018113 2215 80-81 -3.455514805223949 2215 82-83 -2.427567188092052 2215 84-85 -5.214443238322204 2215 86-87 -3.074004015594266 2215 88-89 -4.46867762171599 2215 90-91 -6.147087454592832 2215 92-93 -5.187774308474001 2215 94-95 -4.069301303804931 2215 96-97 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-3.327590576116151 2216 30-31 -2.948843670410355 2216 32-33 -3.3475210126427974 2216 34-35 -3.030959507998105 2216 36-37 -2.664566472380198 2216 38-39 -3.0695169908024873 2216 40-41 -3.19027086438048 2216 42-43 -3.3436451016944666 2216 44-45 -3.19201192215316 2216 46-47 -3.2764335350903586 2216 48-49 -3.0573632779201 2216 50-51 -3.0617019345974903 2216 52-53 -3.420804748079977 2216 54-55 -4.059275907286398 2216 56-57 -3.1507092708569715 2216 58-59 -3.888271811461969 2216 60-61 -3.538617003430648 2216 62-63 -3.604508320251007 2216 64-65 -3.4043943340538725 2216 66-67 -4.249026958454042 2216 68-69 -3.8829315429745463 2216 70-71 -5.810964637526311 2216 72-73 -6.167563051434442 2216 74-75 -4.209806608174581 2216 76-77 -3.608658345374675 2216 78-79 -3.8948542286774135 2216 80-81 -3.9127805730626584 2216 82-83 -4.120301360071135 2216 84-85 -3.9339154122760682 2216 86-87 -4.1208072671185825 2216 88-89 -4.380062931881046 2216 90-91 -4.4719665585680275 2216 92-93 -4.249938817742265 2216 94-95 -7.292283253957212 2216 96-97 -9.011355414015044 2216 98-99 -6.08937082214911 2216 100-101 -5.108711008525063 2216 102-103 -5.96241691632661 2216 104-105 -8.473011274093835 2216 106-107 -5.3692597497203565 2216 108-109 -7.513552251169674 2216 110-111 -5.177941728938595 2216 112-113 -4.632586536492489 2216 114-115 -4.853503873813995 2216 116-117 -4.478195956247159 2216 118-119 -4.777168256676976 2216 120-121 -5.484563608113035 2216 122-123 -8.083018507648298 2216 124-125 -6.678171805336602 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 355581.0 3 50382.0 4 28911.0 5 24841.0 6 24745.0 7 25786.0 8 27640.0 9 29090.0 10 30579.0 11 31750.0 12 32090.0 13 33761.0 14 35286.0 15 29428.0 16 23455.0 17 20663.0 18 18712.0 19 17731.0 20 17421.0 21 18943.0 22 23262.0 23 32228.0 24 42852.0 25 53241.0 26 63077.0 27 69577.0 28 77652.0 29 87343.0 30 95770.0 31 106613.0 32 123872.0 33 146745.0 34 176925.0 35 216608.0 36 268362.0 37 267057.0 38 74005.0 39 178.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3939900125281 20.00688027429646 14.174793975794723 22.424335737380716 2 14.785374630510217 21.329734824144282 40.41004441017293 23.474846135172573 3 18.118941120689165 28.043328306284383 28.995825549976296 24.841905023050163 4 10.749993662065624 17.46566892701806 38.486686945676226 33.29765046524009 5 12.550169397595868 40.19607843137255 33.56496954842543 13.688782622606151 6 29.71719845785492 38.61022793498095 17.905470632257355 13.767102974906775 7 27.299262518713608 34.439787231384976 21.39384498439566 16.867105265505753 8 25.29249616714393 36.582755430950066 19.991806110300605 18.132942291605403 9 26.277796891573384 17.43382780167399 20.75567858177857 35.53269672497406 10-11 23.55162169627268 28.820945027284335 27.24578647497613 20.381646801466854 12-13 24.51573852039314 26.66103248919412 27.66783240368215 21.155396586730586 14-15 22.058603082734727 27.610841324035494 25.720751460312268 24.609804132917503 16-17 21.18263948628071 30.984497756583014 26.191563181547227 21.641299575589056 18-19 21.052132799538544 29.264492557046463 28.725099729580776 20.958274913834213 20-21 22.096537967363506 28.359182602875492 28.80774722289275 20.736532206868247 22-23 21.786479271597802 28.39268371121333 28.38283233492966 21.43800468225921 24-25 21.356172795771712 28.594669558719783 28.75282350843711 21.296334137071398 26-27 21.29706263287711 28.803296726436162 28.40965748264322 21.489983158043508 28-29 21.250260752040113 28.417359581569034 28.737007232177543 21.59537243421331 30-31 21.211565350769817 28.395496368073303 28.842840390079704 21.550097891077172 32-33 21.28526597660135 28.56819358755327 28.359464474294217 21.787075961551157 34-35 21.584034971463613 28.229598209736334 28.792883776886658 21.393483041913395 36-37 21.66766444360181 27.97416188967948 28.553288872036227 21.80488479468249 38-39 21.36164678472107 28.340373864330704 28.48686521011666 21.81111414083156 40-41 21.4907837177705 28.187055152043488 28.81659823263857 21.505562897547442 42-43 21.45469711552275 28.344683868225175 28.327497895476807 21.87312112077527 44-45 21.311519045728826 28.383161198326896 28.468731476892987 21.836588279051288 46-47 21.672884795337083 28.515532711734597 27.943695659777116 21.867886833151204 48-49 21.53679039523402 28.625227036763807 27.75771371792799 22.080268850074184 50-51 21.647356318967386 28.665163478414495 27.594931513327488 22.09254868929063 52-53 21.90253626292045 28.660913140311806 27.5333361886814 21.903214408086345 54-55 21.732758016094202 28.63590459974806 27.552428933158186 22.078908450999556 56-57 21.529739077428438 28.909604932116306 27.42824981713703 22.132406173318227 58-59 21.667696994963052 28.30738952405971 27.692012266855503 22.33290121412173 60-61 21.70218713302276 28.595232328107617 27.73820018967017 21.964380349199452 62-63 21.63637697139689 28.5966868022965 27.835582479200387 21.931353747106222 64-65 21.531173138738442 28.498279842401118 27.65826761508656 22.31227940377387 66-67 21.52321686178524 28.816357981730878 27.29811277118341 22.362312385300473 68-69 21.618871565577532 28.622722690623192 27.601400973657775 22.157004770141494 70-71 21.44096299029772 28.44006934991945 27.967339299976945 22.151628359805883 72-73 21.708449658242976 28.53884374675471 27.431153437537137 22.321553157465175 74-75 21.445967674158545 28.987962165122273 27.407714185331233 22.15835597538795 76-77 21.63589953702977 28.62713550908881 27.692129791556326 22.044835162325104 78-79 21.633693751223667 28.569133895873893 27.618327430875382 22.178844922027054 80-81 21.600640817345464 29.057351475073666 27.287609252737237 22.05439845484363 82-83 21.858560919796204 28.819041460207877 27.33687436028111 21.985523259714803 84-85 21.684664402027718 28.769884215768688 27.232036510210055 22.313414871993533 86-87 21.57135586893198 28.634013388883616 27.512360699008504 22.282270043175902 88-89 21.816622843137342 28.496310168873013 27.49668758359111 22.190379404398534 90-91 21.754765268863984 28.710100275054216 27.529815384977763 22.005319071104033 92-93 21.735971271161866 28.67746525996476 27.383418464077803 22.203145004795573 94-95 21.55080316007117 28.385180648784246 27.4902744461105 22.573741745034084 96-97 21.872305588388123 28.255866322923985 27.391085102009356 22.480742986678543 98-99 21.49675479304389 28.30957488172522 27.453364146427507 22.740306178803383 100-101 21.529151641281505 28.441557445075745 27.37760939393415 22.6516815197086 102-103 21.731320422626315 28.085453837230734 27.653463267151285 22.529762472991667 104-105 21.501279869952008 28.25089198325864 27.61185813562785 22.6359700111615 106-107 21.411996042299926 28.313043726519 27.70889670634775 22.566063524833318 108-109 21.509897695206874 28.432859869876953 27.155040278475166 22.902202156441003 110-111 21.27583453407504 28.510440876667843 27.433743592946332 22.779980996310787 112-113 21.271571648896504 28.576440246771224 27.695096605905327 22.45689149842694 114-115 21.289413348403087 28.786235798721698 27.34459270531307 22.579758147562142 116-117 21.036274829603734 28.747183259945224 27.58064067140662 22.635901239044426 118-119 21.148510067133255 28.600048036447745 27.36482254529648 22.886619351122523 120-121 21.10317339370075 28.765400418922088 27.48024324462259 22.651182942754573 122-123 21.168369696741507 28.799519488739445 27.47681088844798 22.555299926071072 124-125 21.250765914963086 28.81377447178648 27.19049894093064 22.7449606723198 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4136.0 1 4386.5 2 4963.0 3 4518.5 4 3135.5 5 2280.0 6 2002.5 7 2197.0 8 2669.0 9 2890.5 10 2641.0 11 2408.0 12 2442.0 13 2630.0 14 2851.5 15 3074.0 16 3277.0 17 3457.0 18 3874.0 19 4445.0 20 5030.0 21 5847.0 22 6994.5 23 8477.0 24 10296.5 25 13076.5 26 17071.5 27 21503.0 28 25536.5 29 30953.0 30 38419.0 31 46007.0 32 54987.0 33 64135.0 34 72652.5 35 79530.0 36 87448.5 37 95123.0 38 97690.5 39 98489.5 40 97055.0 41 95363.5 42 94431.0 43 94245.5 44 95703.5 45 96177.5 46 93539.0 47 89428.5 48 87171.5 49 85289.0 50 82311.5 51 79143.0 52 76889.5 53 75855.0 54 74271.5 55 70975.5 56 66750.0 57 62380.0 58 58829.0 59 54171.0 60 46929.5 61 39228.5 62 32329.5 63 25500.5 64 19460.5 65 15292.5 66 12000.0 67 9367.0 68 7064.5 69 5119.5 70 3920.0 71 3131.5 72 2502.0 73 2085.5 74 1747.5 75 1388.0 76 1043.5 77 748.0 78 513.5 79 360.5 80 258.0 81 170.5 82 134.0 83 99.5 84 75.5 85 57.5 86 47.0 87 29.0 88 15.0 89 12.5 90 11.0 91 10.5 92 10.5 93 12.5 94 8.0 95 3.0 96 2.5 97 4.0 98 3.5 99 0.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10188561546405953 2 0.03575810392118657 3 0.04689236382478957 4 0.05584973317031634 5 0.05888310525943896 6 0.0032474924718842095 7 0.0020341436362351644 8 0.002640818054059687 9 0.00820794800586119 10-11 0.062291188018394374 12-13 0.007672647048957198 14-15 0.012989969887536838 16-17 0.022554013650888136 18-19 0.020912424049715897 20-21 0.13530623854009868 22-23 0.018664160030719138 24-25 0.016469426107412775 26-27 0.008243634736321455 28-29 0.00608458754347536 30-31 0.0024266976712980903 32-33 0.007315779744354538 34-35 0.020109472614359913 36-37 0.0035508296807964706 38-39 0.010349151833477151 40-41 0.03297453894528582 42-43 0.01636236591603198 44-45 0.11751640340565606 46-47 0.004996142264437245 48-49 0.01081307932946061 50-51 0.01211564499126032 52-53 0.014346065645026948 54-55 0.012329765374021915 56-57 0.007155189457283341 58-59 0.01663001639448397 60-61 0.03509789940767165 62-63 0.008796779058455578 64-65 0.008511285214773451 66-67 0.009849537607033427 68-69 0.00904658617167744 70-71 0.004996142264437245 72-73 8.921682615066509E-4 74-75 0.0013739391227202424 76-77 0.009064429536907573 78-79 0.023035784512101726 80-81 0.0041218173681607274 82-83 0.005353009569039906 84-85 0.0034437694894156727 86-87 0.0048533953425961805 88-89 0.007297936379124404 90-91 0.00603105744778496 92-93 0.0035508296807964706 94-95 0.004871238707826314 96-97 0.0023553242103775585 98-99 0.003800636794018333 100-101 0.0035151429503362044 102-103 6.245177830546556E-4 104-105 0.004264564290001791 106-107 0.001052758548577848 108-109 0.0016059028707119717 110-111 0.0026586614192898195 112-113 0.004764178516445516 114-115 0.002908468532511682 116-117 0.005852623795483631 118-119 0.004389467846612722 120-121 0.007458526666195601 122-123 0.005228106012428974 124-125 0.056741901431823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2802162.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.049180304083144 #Duplication Level Percentage of deduplicated Percentage of total 1 81.4370405683973 45.64479370249034 2 8.852898301809187 9.923953862636292 3 3.6192553782566 6.085688917872805 4 1.8564054728930361 4.16200020270674 5 1.0742536071875408 3.0105517060783096 6 0.6950196047721039 2.3373167485646555 7 0.4458948016139226 1.749442669261849 8 0.32485473775748375 1.4566273415363884 9 0.2534422417808198 1.2784706915619786 >10 1.27863594091991 12.870044165087444 >50 0.10111939765663996 3.9065654241439867 >100 0.058188459649534605 5.922762374428495 >500 0.002515568870894912 0.8919684816684547 >1k 4.079300871698545E-4 0.5579232938884209 >5k 6.798834786164242E-5 0.20189041807396035 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5298 0.18906829797848945 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4911 0.1752575332903665 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3576 0.12761574812591134 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.5686730460266035E-5 0.0 3 0.0 0.0 0.0 3.5686730460266035E-5 0.0 4 0.0 0.0 0.0 7.137346092053207E-5 0.0 5 0.0 0.0 0.0 7.137346092053207E-5 0.0 6 0.0 0.0 0.0 7.137346092053207E-5 0.0 7 0.0 0.0 0.0 1.0706019138079811E-4 0.0 8 0.0 0.0 0.0 1.0706019138079811E-4 0.0 9 0.0 0.0 0.0 1.0706019138079811E-4 0.0 10-11 3.5686730460266035E-5 0.0 0.0 1.0706019138079811E-4 0.0 12-13 5.3530095690399056E-5 0.0 0.0 1.0706019138079811E-4 0.0 14-15 7.137346092053207E-5 0.0 0.0 1.249035566109311E-4 0.0 16-17 1.2490355661093114E-4 0.0 0.0 3.747106698327934E-4 0.0 18-19 1.9627701753146322E-4 0.0 0.0 4.460841307533255E-4 0.0 20-21 2.1412038276159623E-4 0.0 0.0 8.92168261506651E-4 0.0 22-23 2.1412038276159623E-4 0.0 0.0 0.0016772763316325037 0.0 24-25 2.854938436821283E-4 0.0 0.0 0.003122588915273278 0.0 26-27 2.854938436821283E-4 0.0 0.0 0.003889853620168998 0.0 28-29 3.390239393725274E-4 0.0 0.0 0.0058704671607137625 0.0 30-31 3.568673046026604E-4 0.0 0.0 0.010152874815945689 0.0 32-33 3.568673046026604E-4 0.0 0.0 0.016754919951094904 3.5686730460266035E-5 34-35 3.747106698327934E-4 0.0 0.0 0.02601562650553394 3.5686730460266035E-5 36-37 4.460841307533255E-4 0.0 0.0 0.04303819693508085 3.5686730460266035E-5 38-39 4.996142264437246E-4 0.0 0.0 0.0701957988153433 3.5686730460266035E-5 40-41 4.996142264437246E-4 0.0 0.0 0.10383054227414404 3.5686730460266035E-5 42-43 5.709876873642567E-4 0.0 0.0 0.13527055180963843 3.5686730460266035E-5 44-45 6.066744178245227E-4 0.0 0.0 0.1720100408184823 3.5686730460266035E-5 46-47 6.780478787450547E-4 0.0 0.0 0.21836710368636789 3.5686730460266035E-5 48-49 7.494213396655868E-4 0.0 0.0 0.2740919333000733 3.5686730460266035E-5 50-51 7.494213396655868E-4 0.0 0.0 0.33729313294520447 7.137346092053207E-5 52-53 7.494213396655868E-4 0.0 0.0 0.398941959815314 7.137346092053207E-5 54-55 7.851080701258528E-4 0.0 0.0 0.46159001513831105 7.137346092053207E-5 56-57 8.921682615066509E-4 0.0 0.0 0.5372815704445353 8.921682615066508E-5 58-59 8.921682615066509E-4 0.0 0.0 0.6240716989239024 1.0706019138079811E-4 60-61 8.921682615066509E-4 0.0 0.0 0.7249759293003045 1.0706019138079811E-4 62-63 8.921682615066509E-4 0.0 0.0 0.8281462670609336 1.4274692184106414E-4 64-65 8.921682615066509E-4 0.0 0.0 0.9314058216477135 1.4274692184106414E-4 66-67 9.27854991966917E-4 0.0 0.0 1.0520983440643332 1.4274692184106414E-4 68-69 9.27854991966917E-4 0.0 0.0 1.1962192050281175 1.4274692184106414E-4 70-71 9.4569835719705E-4 0.0 0.0 1.3629654531037105 1.6059028707119717E-4 72-73 9.99228452887449E-4 0.0 0.0 1.5344045062348286 1.784336523013302E-4 74-75 0.001052758548577848 0.0 0.0 1.7027745005463637 1.784336523013302E-4 76-77 0.0010706019138079811 0.0 0.0 1.8894696309492456 1.784336523013302E-4 78-79 0.0011062886442682472 0.0 0.0 2.1128328769000504 1.784336523013302E-4 80-81 0.0011776621051887792 0.0 0.0 2.3676539757515807 1.784336523013302E-4 82-83 0.0012133488356490453 0.0 0.0 2.640050789354791 2.1412038276159623E-4 84-85 0.0013382523922599765 0.0 0.0 2.908878929911975 2.498071132218623E-4 86-87 0.0014809993141010405 0.0 0.0 3.182667526003136 2.676504784519953E-4 88-89 0.0014988426793311736 0.0 0.0 3.493766598790505 2.854938436821283E-4 90-91 0.0016415896011722378 0.0 0.0 3.8464585559293143 2.854938436821283E-4 92-93 0.0016415896011722378 0.0 0.0 4.232303485665711 2.854938436821283E-4 94-95 0.0016415896011722378 0.0 0.0 4.619468824429137 2.854938436821283E-4 96-97 0.0016415896011722378 3.5686730460266035E-5 0.0 5.000067804787874 2.854938436821283E-4 98-99 0.0018021798882434349 3.5686730460266035E-5 0.0 5.407485363087502 2.854938436821283E-4 100-101 0.0018735533491639669 3.5686730460266035E-5 0.0 5.877015675753222 2.854938436821283E-4 102-103 0.001927083444854366 3.5686730460266035E-5 0.0 6.383107043775485 3.033372089122613E-4 104-105 0.0023910109408378246 7.137346092053207E-5 0.0 6.903062706581561 3.2118057414239434E-4 106-107 0.0024266976712980907 7.137346092053207E-5 0.0 7.40870799047307 3.2118057414239434E-4 108-109 0.0024623844017583564 7.137346092053207E-5 0.0 7.9219188612221565 3.2118057414239434E-4 110-111 0.0025337578626788886 7.137346092053207E-5 0.0 8.502131568410391 3.2118057414239434E-4 112-113 0.002640818054059687 7.137346092053207E-5 0.0 9.138211852134173 3.2118057414239434E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9755 0.0 54.003536 1 GGTATCA 5110 0.0 52.536583 1 ATCAACG 12005 0.0 41.58264 3 TCAACGC 12115 0.0 40.77147 4 CAACGCA 12315 0.0 40.542595 5 TATCAAC 13275 0.0 38.948414 2 AACGCAG 12280 0.0 38.84423 6 GTACATG 14300 0.0 38.296402 1 TACATGG 14975 0.0 34.169304 2 CGCAGAG 13645 0.0 33.433254 8 ACGCAGA 14015 0.0 33.272415 7 ACATGGG 14840 0.0 30.752026 3 GCAGAGT 14560 0.0 29.04159 9 CATGGGA 10505 0.0 25.637026 4 GAGTACT 6715 0.0 25.421255 12-13 CGCATAG 455 0.0 24.86942 8 AGAGTAC 13800 0.0 24.368076 10-11 CGCGATA 285 0.0 23.995016 104-105 CAGAGTA 14800 0.0 23.706856 10-11 ACGCATA 405 0.0 23.527761 7 >>END_MODULE