FastQCFastQC Report
Thu 26 May 2016
SRR1364495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364495_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802162
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141620.5053954767782877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128640.45907410064086235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87200.31118828961351985No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30740.1097010094348578No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30130.10752411887678157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82150.073.211461
GTATCAA151150.050.409091
ATCAACG198200.036.718053
TATCAAC207300.036.400052
TCAACGC199550.036.0514834
CAACGCA200100.035.7919775
AACGCAG203250.034.710466
GTACATG142000.034.5811
TACATGG151250.031.6437362
CGCAGAG235750.029.9914118
ACGCAGA235250.029.7108127
ACATGGG146200.029.5976223
GAGTACT166700.028.00475912-13
GCAGAGT245550.027.7530969
GTACTTT178150.026.72250414-15
CAGAGTA244750.025.79259710-11
AGTACTT174900.025.29769112-13
AGCGTAC4050.024.9689435
CATGGGA98900.024.829534
AGAGTAC237900.024.56042510-11
Produced by FastQC (version 0.11.5)