##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364495_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2802162 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.827504262779954 31.0 28.0 31.0 25.0 34.0 2 28.98539163688609 31.0 28.0 33.0 25.0 34.0 3 29.097976134142137 31.0 28.0 34.0 25.0 34.0 4 32.51804785019567 35.0 32.0 37.0 26.0 37.0 5 31.6215051092692 35.0 32.0 37.0 19.0 37.0 6 31.664887326285918 35.0 32.0 37.0 19.0 37.0 7 31.110077147573907 35.0 31.0 37.0 17.0 37.0 8 31.220363419388317 35.0 31.0 37.0 17.0 37.0 9 31.733240976074903 37.0 30.0 39.0 10.0 39.0 10-11 31.939852513880354 37.0 30.5 39.0 12.0 39.0 12-13 31.675982330786013 37.0 30.0 39.0 10.5 39.0 14-15 32.593487100317546 37.0 31.0 40.0 10.5 41.0 16-17 32.67601944498569 37.5 31.0 40.0 11.0 41.0 18-19 32.58734505713802 38.0 31.0 40.0 10.0 41.0 20-21 32.48761670453029 38.0 30.5 40.0 10.0 41.0 22-23 32.457438220916565 37.0 30.5 40.0 10.0 41.0 24-25 32.250720871955295 37.0 30.0 40.0 9.5 41.0 26-27 32.32734278032462 37.0 30.0 40.0 9.5 41.0 28-29 32.19437776973636 37.0 30.0 40.0 8.0 41.0 30-31 31.620464841076284 37.0 30.0 40.0 7.5 41.0 32-33 31.488587740466112 36.0 30.0 40.0 2.0 41.0 34-35 31.247268894517877 36.0 30.0 40.0 2.0 41.0 36-37 31.19441060152839 36.0 30.0 40.0 2.0 41.0 38-39 30.94069757565765 36.0 29.0 40.0 2.0 41.0 40-41 30.626714479748138 35.5 28.5 40.0 2.0 41.0 42-43 30.47093137370359 35.5 28.0 40.0 2.0 41.0 44-45 30.333960527621173 35.0 28.0 40.0 2.0 41.0 46-47 30.09760731178283 35.0 27.5 40.0 2.0 41.0 48-49 29.743682734973923 35.0 27.0 40.0 2.0 41.0 50-51 29.38335792149062 35.0 26.0 40.0 2.0 41.0 52-53 29.291268491971557 35.0 26.0 40.0 2.0 41.0 54-55 28.833373837772406 35.0 24.0 39.0 2.0 41.0 56-57 28.857648130265133 35.0 25.0 39.0 2.0 41.0 58-59 28.618234955723473 35.0 24.5 39.0 2.0 41.0 60-61 28.34266345057852 34.0 23.5 39.0 2.0 41.0 62-63 28.086032320758044 34.0 23.0 39.0 2.0 40.0 64-65 27.61926683753473 34.0 22.0 38.0 2.0 40.0 66-67 27.236117861850957 33.5 21.0 38.0 2.0 40.0 68-69 27.049478581181248 33.5 20.5 37.0 2.0 40.0 70-71 27.126447543004293 33.5 22.0 37.0 2.0 40.0 72-73 26.898243570500206 33.5 21.5 36.5 2.0 39.0 74-75 26.529818582937033 33.0 20.0 36.0 2.0 39.0 76-77 26.452486151764244 33.0 20.5 36.0 2.0 39.0 78-79 26.051884223681572 33.0 20.0 35.0 2.0 37.5 80-81 25.82202777712352 33.0 18.5 35.0 2.0 37.0 82-83 25.678546600803237 33.0 18.5 35.0 2.0 37.0 84-85 25.235287431633147 32.5 14.0 35.0 2.0 36.0 86-87 25.11116041827703 32.0 14.0 35.0 2.0 36.0 88-89 25.05178430083628 32.0 16.0 35.0 2.0 36.0 90-91 24.882141717716536 32.0 13.0 35.0 2.0 35.0 92-93 24.89308148493913 32.0 14.0 35.0 2.0 35.0 94-95 24.900180289362286 32.0 15.0 35.0 2.0 35.0 96-97 24.774684333025714 32.0 11.0 35.0 2.0 35.0 98-99 24.63476683360919 32.0 8.5 35.0 2.0 35.0 100-101 24.53939547392335 32.0 7.0 35.0 2.0 35.0 102-103 23.42864188437357 30.0 7.0 34.0 2.0 35.0 104-105 24.347480445456043 32.0 7.0 35.0 2.0 35.0 106-107 24.441473226744208 32.0 7.0 35.0 2.0 35.0 108-109 24.50708506503193 32.0 3.5 35.0 2.0 35.0 110-111 24.42934455609633 32.0 2.0 35.0 2.0 35.0 112-113 24.374162700086575 32.0 2.0 35.0 2.0 35.0 114-115 24.31917230338574 32.0 2.0 35.0 2.0 35.0 116-117 24.235422327474286 32.5 2.0 35.0 2.0 35.0 118-119 24.107915959177234 32.5 2.0 35.0 2.0 35.0 120-121 23.942199808576376 32.0 2.0 35.0 2.0 35.0 122-123 23.65539305008062 32.0 2.0 35.0 2.0 35.0 124-125 22.910463420744414 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8946330491862469 1101 2 0.8790490359639591 1101 3 0.933568242800682 1101 4 0.9639668531240844 1101 5 1.1147538067441793 1101 6 1.0743300784950733 1101 7 1.581779393673294 1101 8 1.4499538156471772 1101 9 2.0580577557159785 1101 10-11 1.6778415837189549 1101 12-13 1.956811778985891 1101 14-15 1.938693478816056 1101 16-17 1.8123423750258851 1101 18-19 1.9367218156848693 1101 20-21 2.1231378872322964 1101 22-23 2.1472740490777014 1101 24-25 2.286896372537825 1101 26-27 2.0067726836466377 1101 28-29 2.081527079398292 1101 30-31 2.495014084116562 1101 32-33 2.2591992869354804 1101 34-35 2.309768691339869 1101 36-37 2.1963069392571626 1101 38-39 2.349850448374692 1101 40-41 2.5194830280907006 1101 42-43 2.427126708173745 1101 44-45 2.267638464809451 1101 46-47 2.0993072588156636 1101 48-49 2.1194938184627077 1101 50-51 2.372551428762762 1101 52-53 2.171928879300097 1101 54-55 2.56511555453568 1101 56-57 2.2865957889824813 1101 58-59 2.3689221011869748 1101 60-61 2.5282764592122113 1101 62-63 2.3565321574650433 1101 64-65 2.5053348022803767 1101 66-67 2.685639061407514 1101 68-69 2.618372366347998 1101 70-71 2.2455655543543536 1101 72-73 2.4340229641937476 1101 74-75 2.5504556920404298 1101 76-77 2.3102861567531043 1101 78-79 2.3921014787342862 1101 80-81 2.362214241782329 1101 82-83 2.326687178262816 1101 84-85 2.6175017480546963 1101 86-87 2.4922255658642634 1101 88-89 2.3429947026903335 1101 90-91 2.3659260177691053 1101 92-93 2.270380706701001 1101 94-95 2.129505070700752 1101 96-97 1.9982369238897775 1101 98-99 2.0938388707017737 1101 100-101 2.144613780512163 1101 102-103 1.951284886071015 1101 104-105 1.9544097736630732 1101 106-107 2.075777187061945 1101 108-109 2.1140357818653506 1101 110-111 2.037655718919094 1101 112-113 1.9336009511476888 1101 114-115 2.0243554077161896 1101 116-117 2.0339945423517847 1101 118-119 2.007415972418137 1101 120-121 1.9655866305977767 1101 122-123 2.076221929025479 1101 124-125 1.8822445133952712 1102 1 0.5050689790656797 1102 2 -1.6700482520414077 1102 3 0.04941317257749844 1102 4 0.4665228904690295 1102 5 0.9941772608885806 1102 6 0.471690846968464 1102 7 1.2461946513674818 1102 8 0.916352504045534 1102 9 1.6681170432311703 1102 10-11 1.4165760759757937 1102 12-13 1.4902080203219477 1102 14-15 1.6374383682254674 1102 16-17 1.4743637858669132 1102 18-19 1.5851495961811324 1102 20-21 1.7425742233300099 1102 22-23 1.7269433437250328 1102 24-25 1.9262449307173526 1102 26-27 1.6230411638220872 1102 28-29 1.654940591371549 1102 30-31 1.9041342462816147 1102 32-33 1.6943817650196245 1102 34-35 1.9241145781465931 1102 36-37 1.3451938354526973 1102 38-39 1.2220887577264428 1102 40-41 1.4032870036022658 1102 42-43 1.194762539000365 1102 44-45 1.1861429370267516 1102 46-47 0.9862281539545279 1102 48-49 1.1169721803429766 1102 50-51 1.2943697813483546 1102 52-53 1.125360967915043 1102 54-55 1.4184413394740538 1102 56-57 1.1000780485228816 1102 58-59 1.0730404251931205 1102 60-61 1.0226327071291799 1102 62-63 1.382528855139423 1102 64-65 1.4397185716976537 1102 66-67 1.676544945127322 1102 68-69 1.613200927817985 1102 70-71 0.8590947643318891 1102 72-73 1.2596473551843523 1102 74-75 1.3982073714010639 1102 76-77 1.2495425843728931 1102 78-79 1.2376163604009527 1102 80-81 1.2355327610107025 1102 82-83 0.8732636762033117 1102 84-85 1.0106858993510777 1102 86-87 1.0608069142545276 1102 88-89 1.2306276458901628 1102 90-91 0.8975788703288288 1102 92-93 0.9704962647762194 1102 94-95 0.925193125169617 1102 96-97 1.0237086226030243 1102 98-99 0.7384445209520756 1102 100-101 0.6013197319067451 1102 102-103 0.4984465948676302 1102 104-105 0.8536347257267707 1102 106-107 1.0254466682813437 1102 108-109 0.8833598248990455 1102 110-111 0.8002904112320692 1102 112-113 0.8284993923380526 1102 114-115 1.1218629002808669 1102 116-117 0.678167260596581 1102 118-119 0.33480119031311517 1102 120-121 0.6378086042928075 1102 122-123 1.0198217226089277 1102 124-125 1.1348849852021061 1103 1 0.7313543512770657 1103 2 0.6861072653003824 1103 3 0.5503783086782477 1103 4 0.6668284225415633 1103 5 0.875330818048031 1103 6 0.7849624885031403 1103 7 1.1378448514342665 1103 8 1.0483285366236572 1103 9 1.6837659212698028 1103 10-11 1.4120363577677573 1103 12-13 1.4891955086970547 1103 14-15 1.3136456215624221 1103 16-17 1.2513817748928773 1103 18-19 1.3802373151420468 1103 20-21 1.6237944907822026 1103 22-23 1.4748059972201233 1103 24-25 1.809606390263781 1103 26-27 1.3245883405506262 1103 28-29 1.5433470902466127 1103 30-31 2.0133451377597282 1103 32-33 1.8846858272224658 1103 34-35 1.9773183065736362 1103 36-37 1.8635920962208274 1103 38-39 1.9860337192246185 1103 40-41 2.2326543592917254 1103 42-43 2.1158185720143194 1103 44-45 1.9862528354241533 1103 46-47 1.921083496770656 1103 48-49 1.8538207455215527 1103 50-51 1.9229294350914898 1103 52-53 1.728373997839924 1103 54-55 2.0025825670331727 1103 56-57 1.840162570303555 1103 58-59 1.9603803071377968 1103 60-61 1.9607736253319992 1103 62-63 1.9336520663261574 1103 64-65 2.0819828376056293 1103 66-67 2.331826477517758 1103 68-69 2.3863675057156293 1103 70-71 2.0806766603227977 1103 72-73 2.0881006356536496 1103 74-75 2.0776660171084167 1103 76-77 2.0294041196560677 1103 78-79 2.226653065910206 1103 80-81 2.17001027467483 1103 82-83 1.9655902940694503 1103 84-85 2.149142497709395 1103 86-87 2.1129015640356386 1103 88-89 2.0479110382639725 1103 90-91 2.014425553305067 1103 92-93 1.8630182919914553 1103 94-95 1.7230657985613647 1103 96-97 1.5499234175679497 1103 98-99 1.7470356743447297 1103 100-101 1.7358728434478508 1103 102-103 1.670385137222631 1103 104-105 1.6586789959375352 1103 106-107 1.6008592092181786 1103 108-109 1.6300743563342799 1103 110-111 1.7137101720492325 1103 112-113 1.5779926291214572 1103 114-115 1.4168705027973374 1103 116-117 1.511683419656137 1103 118-119 1.5821936671012438 1103 120-121 1.6310557494234281 1103 122-123 1.820864657118328 1103 124-125 1.5974859880036192 1104 1 0.6805904635234299 1104 2 0.5921163460981695 1104 3 0.5419108492127656 1104 4 0.5250385886999425 1104 5 0.8825894621525698 1104 6 0.9474511284238929 1104 7 1.394638950792654 1104 8 1.1184425521611558 1104 9 1.5973724050974667 1104 10-11 1.195048702639479 1104 12-13 1.2464263013081727 1104 14-15 1.2569337749296992 1104 16-17 1.174364070425078 1104 18-19 1.1119989765926093 1104 20-21 1.4031829628068806 1104 22-23 1.5161285314396267 1104 24-25 1.5259767715325623 1104 26-27 1.43985204878323 1104 28-29 1.3720790238607563 1104 30-31 1.8755456358170761 1104 32-33 1.9013729586821135 1104 34-35 1.7610606935798288 1104 36-37 1.6722426489821558 1104 38-39 1.802935579643826 1104 40-41 1.9417489920062074 1104 42-43 1.867198885973309 1104 44-45 1.6316072063224851 1104 46-47 1.7116175612662374 1104 48-49 1.4245635387962636 1104 50-51 1.7902085924263957 1104 52-53 1.580861287568716 1104 54-55 1.9013398152329657 1104 56-57 1.8455067269694183 1104 58-59 1.916155919764698 1104 60-61 1.9954125579658957 1104 62-63 2.0184633452122434 1104 64-65 2.236538191540589 1104 66-67 2.1936944226890667 1104 68-69 2.285013196574951 1104 70-71 2.0775182404227834 1104 72-73 2.1652772752740965 1104 74-75 2.2688559237272017 1104 76-77 2.124363180988084 1104 78-79 2.145618388984502 1104 80-81 2.033369939778783 1104 82-83 2.1345790789996144 1104 84-85 2.3085810963675577 1104 86-87 2.2051074144234697 1104 88-89 2.0363193677980007 1104 90-91 2.1353154145743574 1104 92-93 1.9053012486333394 1104 94-95 1.8365760736433003 1104 96-97 1.809531123228524 1104 98-99 1.8912419279737485 1104 100-101 1.9761672034598874 1104 102-103 1.8994803209123141 1104 104-105 1.795393046471041 1104 106-107 1.9086266879980656 1104 108-109 1.9004363712566281 1104 110-111 1.8402626657569137 1104 112-113 1.776639773102172 1104 114-115 1.7277425148921495 1104 116-117 1.7872044696332239 1104 118-119 1.6808525874199347 1104 120-121 1.6782522241509241 1104 122-123 1.8216870330081392 1104 124-125 1.5973615644841246 1105 1 0.2049940446491867 1105 2 0.36431265338504204 1105 3 0.2633054751218147 1105 4 0.3960116389689077 1105 5 0.5691217435136942 1105 6 0.7119621409201216 1105 7 1.0458239209709603 1105 8 0.8565722805858513 1105 9 1.3396812546725272 1105 10-11 1.1066924362399497 1105 12-13 1.3511167844910865 1105 14-15 1.1774837781427934 1105 16-17 1.095568403234104 1105 18-19 1.2724206843598225 1105 20-21 1.4623838759254895 1105 22-23 1.3795221387685999 1105 24-25 1.6526443510775977 1105 26-27 1.4172458470959626 1105 28-29 1.499992652354102 1105 30-31 1.8029659496430455 1105 32-33 1.6420153547956566 1105 34-35 1.7799293490000707 1105 36-37 1.443453708263398 1105 38-39 1.5757404224635927 1105 40-41 1.6818462135527774 1105 42-43 1.7758930314801162 1105 44-45 1.6241592899373387 1105 46-47 1.436719254115097 1105 48-49 1.4999045318998299 1105 50-51 1.5467488854963847 1105 52-53 1.4504486545332114 1105 54-55 1.695166235014078 1105 56-57 1.4326864613097996 1105 58-59 1.3862151607663904 1105 60-61 1.2379721488042001 1105 62-63 1.1058984752944099 1105 64-65 1.504510934914265 1105 66-67 1.6972448889477008 1105 68-69 1.783751532601876 1105 70-71 1.5839082388792995 1105 72-73 1.713615085915496 1105 74-75 1.7920329746856325 1105 76-77 1.6346674457516421 1105 78-79 1.8071334487750335 1105 80-81 1.7983294338039357 1105 82-83 1.6894226494831344 1105 84-85 1.9475638255495795 1105 86-87 1.8744448509048546 1105 88-89 1.752448427178937 1105 90-91 1.7973095853654932 1105 92-93 1.5546529548450572 1105 94-95 1.396002503754353 1105 96-97 1.5345885234590284 1105 98-99 1.5865824346092339 1105 100-101 1.5666040217664623 1105 102-103 1.4444054979176286 1105 104-105 1.0590494366496728 1105 106-107 1.283507998795315 1105 108-109 1.312747647166308 1105 110-111 1.374729679917941 1105 112-113 1.4860908569350357 1105 114-115 1.3538595146693382 1105 116-117 1.2792992131954755 1105 118-119 1.3400477650198255 1105 120-121 1.4764170683640216 1105 122-123 1.5599879261274978 1105 124-125 1.5018075223848157 1106 1 0.24121632687968741 1106 2 0.262123686373009 1106 3 0.28634007043734755 1106 4 0.1135207594945058 1106 5 0.4013403431024152 1106 6 0.4832753382417323 1106 7 0.7268280528574351 1106 8 0.44883418625999383 1106 9 1.214395572541406 1106 10-11 0.714722529402291 1106 12-13 0.7625779546585854 1106 14-15 0.753910395702114 1106 16-17 0.866099355907707 1106 18-19 0.957678183716915 1106 20-21 0.8097912527952005 1106 22-23 0.7354055738180065 1106 24-25 0.5601494195120722 1106 26-27 0.7123854628870205 1106 28-29 0.6626283006002964 1106 30-31 1.0782283777161297 1106 32-33 0.8566017028040811 1106 34-35 0.9470433702060106 1106 36-37 0.9592061359165527 1106 38-39 1.0115860676252595 1106 40-41 1.25940784574653 1106 42-43 1.2858698755059734 1106 44-45 1.1581486564164933 1106 46-47 1.084803287292381 1106 48-49 0.9834072285429798 1106 50-51 1.3459974504318595 1106 52-53 1.0573564277742662 1106 54-55 1.550393887235952 1106 56-57 1.240684365583796 1106 58-59 1.1690803937938128 1106 60-61 1.3152247656264677 1106 62-63 1.4375640502668325 1106 64-65 1.5358781917905837 1106 66-67 1.60063329434767 1106 68-69 1.5184610699023153 1106 70-71 1.4333861311257543 1106 72-73 1.3846709815803813 1106 74-75 1.6251711108401885 1106 76-77 1.3104608193463712 1106 78-79 1.2235961315801802 1106 80-81 1.2947353995937014 1106 82-83 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-4.440324570407931 2216 110-111 -4.143447725551614 2216 112-113 -4.269646651293463 2216 114-115 -4.029189844984245 2216 116-117 -3.946388480098477 2216 118-119 -4.170311343709017 2216 120-121 -4.097704990350792 2216 122-123 -4.128224061529853 2216 124-125 -3.988988047856548 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 148415.0 3 48823.0 4 23357.0 5 19619.0 6 21122.0 7 25006.0 8 27094.0 9 28185.0 10 29716.0 11 32343.0 12 36272.0 13 40264.0 14 43251.0 15 34624.0 16 23039.0 17 19840.0 18 17271.0 19 16173.0 20 15961.0 21 16878.0 22 20374.0 23 26954.0 24 36923.0 25 48532.0 26 58995.0 27 66596.0 28 73662.0 29 84115.0 30 92688.0 31 104781.0 32 123018.0 33 145310.0 34 177180.0 35 225736.0 36 308468.0 37 395385.0 38 145586.0 39 606.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.52344185408432 21.067194551383732 14.523653350939018 22.885710243592932 2 15.56210764628274 21.57557797398363 38.7906610581341 24.071653321599523 3 18.09592022160032 27.124448907664867 30.5009845968934 24.27864627384141 4 12.213587074856443 18.018625175055693 37.36347264733549 32.40431510275237 5 12.110862969378644 39.92809837546866 34.058559069746856 13.90247958540584 6 29.819032460673196 39.07728323864447 17.072651097724613 14.031033202957719 7 26.36407742901014 34.330250343708066 21.520283863053713 17.785388364228087 8 26.003814197751595 35.93407518908614 19.411011925791584 18.65109868737068 9 26.094351432929287 15.859754004229593 20.747301547876244 37.29859301496488 10-11 24.290568234172703 27.89594156174377 27.06455822823795 20.74893197584558 12-13 25.380147366099514 25.639877707621007 27.86598228423521 21.11399264204427 14-15 22.518065188310306 26.372703610646635 26.678059135800865 24.431172065242198 16-17 20.896676670742885 29.72582859286205 27.427722348609013 21.949772387786044 18-19 20.731599601942325 27.191846562577123 31.1144847635163 20.962069071964255 20-21 21.625443133722488 26.360690641761657 31.416505817772318 20.597360406743537 22-23 21.289591331063843 26.218988850136167 31.280876429331318 21.210543389468672 24-25 20.955863655757177 26.638069343423233 31.40402221904593 21.00204478177366 26-27 20.790281887156272 26.816154956477018 31.42028864476786 20.97327451159884 28-29 20.71730479574213 26.390384069426105 31.785506976388966 21.106804158442802 30-31 20.679136093979817 26.343266657352338 31.609325537130907 21.36827171153694 32-33 20.745595988239078 26.555701117815854 31.287442084876638 21.41126080906843 34-35 21.123046322614005 26.50088620417509 31.368540849167925 21.00752662404298 36-37 21.270188739307645 26.238089817027756 31.11436380824654 21.377357635418054 38-39 20.942539166891347 26.555688529156342 31.099228144454415 21.4025441594979 40-41 20.994404749138017 26.390948102116674 31.297913696061375 21.316733452683934 42-43 21.19738135058972 26.6057106884527 30.65685889473781 21.54004906621977 44-45 21.11997145023866 26.711192398626043 30.549261721015302 21.619574430119997 46-47 21.520180818074387 26.939548457998463 29.815318825324876 21.72495189860227 48-49 21.478669234446336 27.232798680589966 29.255505080631504 22.033027004332194 50-51 21.60713590806676 27.214798058828503 28.93045991969265 22.247606113412083 52-53 21.876417433842978 27.366148744593012 28.818457482248512 21.938976339315506 54-55 21.63373442394529 27.285722428571763 28.794149762770992 22.286393384711957 56-57 21.51131512865721 27.531734052589158 28.646090482844365 22.310860335909265 58-59 21.54971521968767 27.062034287835097 28.97288163416793 22.415368858309304 60-61 21.634777004327375 27.367475542099278 28.956730553051536 22.04101690052181 62-63 21.55944084144291 27.264957234455533 29.234898768405138 21.94070315569642 64-65 21.398302663773734 27.058500464935577 29.00935513221739 22.5338417390733 66-67 21.350208228484142 27.42972656878342 28.64942578498309 22.570639417749348 68-69 21.543881650169812 27.203869812617288 28.860859509093096 22.391389028119807 70-71 21.511823775742613 27.24468303030692 28.97105207371989 22.272441120230575 72-73 21.665819667359624 27.30642434986702 28.44494498552006 22.582810997253294 74-75 21.366993513619615 27.712957593169225 28.414414575173314 22.50563431803785 76-77 21.523228498549752 27.42919391921973 28.69111909518104 22.35645848704948 78-79 21.544830633168612 27.403320280088632 28.50158077136789 22.55026831537486 80-81 21.415085636753563 27.73953836608996 28.367983147259313 22.47739284989716 82-83 21.675934530411585 27.545325813409388 28.306703838044704 22.472035818134323 84-85 21.346642342591185 27.473768468775184 28.215338727739507 22.964250460894124 86-87 21.360466969088616 27.36041950562701 28.454291705082035 22.82482182020234 88-89 21.554067038920728 27.2904054557293 28.374888499540617 22.780639005809356 90-91 21.482036742851605 27.547449717636507 28.392550649938325 22.57796288957356 92-93 21.4202599962849 27.543286171318897 28.330541060858934 22.70591277153727 94-95 21.265851245214403 27.208601628642114 28.377433840317412 23.148113285826067 96-97 21.589588650781415 27.12021643798071 28.2693740685544 23.020820842683477 98-99 21.149437470067756 27.101984110840128 28.316974536090346 23.431603883001767 100-101 21.168724338579967 27.318275659635667 28.275017263745422 23.237982738038944 102-103 21.463128264835905 26.93533896301451 28.37361098100094 23.227921791148642 104-105 21.242126814251964 27.073748814973914 28.371750512596826 23.312373858177292 106-107 21.009471265024125 27.087478944814002 28.41536528963371 23.487684500528164 108-109 21.316808235926402 27.1827967119674 27.7965014156926 23.703893636413596 110-111 21.008394964448804 27.211315144481606 28.050008636207263 23.73028125486233 112-113 21.022811521903563 27.239108708518806 28.31704559181788 23.421034177759754 114-115 20.99588585014017 27.407300479255547 27.956180922670864 23.640632747933424 116-117 20.803026377489953 27.37372071992983 28.056336500173796 23.766916402406427 118-119 20.730208675149143 27.28150955139313 27.960368012838803 24.02791376061893 120-121 20.66289923396328 27.343870724835256 28.08390894637386 23.9093210948276 122-123 20.794644083886038 27.45426255337448 27.87256859501773 23.878524767721753 124-125 20.6301840725519 27.39893818916122 27.84024406195556 24.13063367633132 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1147.0 1 916.5 2 1111.5 3 1581.5 4 1163.5 5 885.0 6 1846.5 7 4224.0 8 6731.0 9 7683.0 10 6074.0 11 3873.5 12 3235.0 13 3149.0 14 3273.5 15 3586.5 16 4026.5 17 4444.5 18 4909.0 19 5339.5 20 5840.0 21 6755.5 22 7880.0 23 9060.5 24 10709.0 25 13445.0 26 17150.5 27 21394.5 28 25400.5 29 30613.5 30 37953.5 31 45093.0 32 53920.0 33 63155.0 34 71228.5 35 77745.5 36 86338.5 37 94661.5 38 96990.0 39 98253.5 40 96449.5 41 94366.0 42 92941.0 43 93015.5 44 95335.0 45 96771.5 46 94834.5 47 89675.5 48 87305.0 49 85948.5 50 83491.0 51 79903.5 52 76976.0 53 75944.0 54 74996.0 55 71778.5 56 66882.5 57 62176.0 58 58968.5 59 54767.5 60 47491.5 61 39731.5 62 32582.0 63 25326.0 64 19170.5 65 14988.0 66 11495.0 67 8968.0 68 6654.5 69 4656.0 70 3525.5 71 2772.0 72 2205.5 73 1994.0 74 1725.5 75 1321.5 76 950.0 77 626.0 78 414.0 79 259.0 80 173.0 81 120.5 82 84.5 83 66.0 84 44.0 85 24.0 86 18.5 87 13.0 88 6.0 89 3.0 90 5.5 91 4.0 92 1.5 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10941551559117567 2 1.0706019138079811E-4 3 0.0 4 0.18539256474108207 5 0.0 6 7.137346092053207E-5 7 0.03900559639307078 8 0.0 9 0.0 10-11 8.921682615066509E-4 12-13 8.921682615066509E-4 14-15 6.602045135149217E-4 16-17 0.00490692543828658 18-19 0.001980613540544765 20-21 0.0036222031417170028 22-23 0.0023196374799172924 24-25 0.005406539664730305 26-27 0.004871238707826314 28-29 0.0036935766026375346 30-31 0.0010170718181175822 32-33 3.5686730460266035E-5 34-35 0.0015166860445613066 36-37 0.0015345294097914397 38-39 6.602045135149217E-4 40-41 0.0019092400796242332 42-43 0.0010884452790381142 44-45 0.0013204090270298435 46-47 3.033372089122613E-4 48-49 1.7843365230133018E-5 50-51 0.04186053482989206 52-53 1.0706019138079811E-4 54-55 0.13976707984763193 56-57 2.3196374799172925E-4 58-59 7.137346092053207E-5 60-61 0.0 62-63 8.207948005861188E-4 64-65 0.06330825983651195 66-67 0.05397617982115238 68-69 0.0022304206537666275 70-71 0.0034259261241855396 72-73 0.07426408608781362 74-75 0.0048890820730564475 76-77 0.001944926810084499 78-79 0.001748649792553036 80-81 2.3196374799172925E-4 82-83 2.1412038276159623E-4 84-85 0.0 86-87 2.3196374799172925E-4 88-89 0.0015523727750215725 90-91 4.103974002930594E-4 92-93 0.002979841993432214 94-95 0.004799865246905782 96-97 0.0023553242103775585 98-99 0.0 100-101 0.001677276331632504 102-103 0.001766493157783169 104-105 3.033372089122613E-4 106-107 7.137346092053207E-5 108-109 0.0 110-111 2.1412038276159623E-4 112-113 1.2490355661093114E-4 114-115 1.2490355661093114E-4 116-117 0.0 118-119 0.00112413200949838 120-121 0.002747878245440485 122-123 9.63541722427183E-4 124-125 0.008939525980296643 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2802162.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.11336020838785 #Duplication Level Percentage of deduplicated Percentage of total 1 76.37061121371462 35.21705504232767 2 10.723317949794689 9.889764464959072 3 4.446881704477513 6.151819735279839 4 2.4072871233116655 4.4403239296913855 5 1.4786858459046697 3.4093586523623363 6 0.9662793716970544 2.6735033237400576 7 0.6359695369798295 2.052868463821876 8 0.47624994160945355 1.756918808532833 9 0.3670218835177788 1.5232151087114671 >10 1.8610430869517363 15.497738501360015 >50 0.15843232799851226 5.084284292897954 >100 0.10156687093765988 8.578723323492992 >500 0.005530926195568098 1.75089696682811 >1k 8.81741857262696E-4 0.6522246449002495 >5k 8.015835066024509E-5 0.32232354227997595 >10k+ 1.6031670132049018E-4 0.9989811988140631 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14162 0.5053954767782877 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12864 0.45907410064086235 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8720 0.31118828961351985 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3074 0.1097010094348578 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3013 0.10752411887678157 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.5686730460266035E-5 0.0 0.0 0.0 0.0 8 3.5686730460266035E-5 0.0 0.0 0.0 0.0 9 3.5686730460266035E-5 0.0 0.0 0.0 0.0 10-11 3.5686730460266035E-5 0.0 0.0 0.0 0.0 12-13 7.137346092053207E-5 3.5686730460266035E-5 0.0 5.3530095690399056E-5 3.5686730460266035E-5 14-15 7.137346092053207E-5 3.5686730460266035E-5 0.0 7.137346092053207E-5 3.5686730460266035E-5 16-17 8.921682615066508E-5 3.5686730460266035E-5 0.0 2.498071132218622E-4 3.5686730460266035E-5 18-19 1.6059028707119717E-4 3.5686730460266035E-5 0.0 3.9255403506292645E-4 3.5686730460266035E-5 20-21 2.1412038276159623E-4 3.5686730460266035E-5 0.0 9.278549919669169E-4 8.921682615066508E-5 22-23 2.1412038276159623E-4 3.5686730460266035E-5 0.0 0.0018913967143941 1.249035566109311E-4 24-25 2.854938436821283E-4 3.5686730460266035E-5 0.0 0.0033367092980348748 1.4274692184106414E-4 26-27 2.854938436821283E-4 3.5686730460266035E-5 0.0 0.004211034194311392 1.4274692184106414E-4 28-29 3.033372089122613E-4 3.5686730460266035E-5 0.0 0.006120274273935625 1.4274692184106414E-4 30-31 3.925540350629264E-4 3.5686730460266035E-5 0.0 0.010670332407619545 1.4274692184106414E-4 32-33 4.103974002930594E-4 3.5686730460266035E-5 0.0 0.017647088212601553 1.4274692184106414E-4 34-35 4.639274959834585E-4 3.5686730460266035E-5 0.0 0.02742525235871445 1.4274692184106414E-4 36-37 5.709876873642566E-4 3.5686730460266035E-5 0.0 0.04455488297964215 1.4274692184106414E-4 38-39 6.066744178245227E-4 3.5686730460266035E-5 0.0 0.07210503889496753 1.4274692184106414E-4 40-41 6.066744178245227E-4 3.5686730460266035E-5 0.0 0.10568625225807787 1.4274692184106414E-4 42-43 6.423611482847887E-4 3.5686730460266035E-5 0.0 0.1378578397680077 1.4274692184106414E-4 44-45 6.780478787450547E-4 3.5686730460266035E-5 0.0 0.17631029183894437 1.784336523013302E-4 46-47 8.029514353559858E-4 3.5686730460266035E-5 0.0 0.2233989326812654 1.784336523013302E-4 48-49 9.27854991966917E-4 3.5686730460266035E-5 0.0 0.2808010386266033 1.784336523013302E-4 50-51 9.27854991966917E-4 3.5686730460266035E-5 0.0 0.34566167123813685 1.784336523013302E-4 52-53 9.63541722427183E-4 3.5686730460266035E-5 0.0 0.4078993291608408 1.784336523013302E-4 54-55 9.992284528874491E-4 3.5686730460266035E-5 0.0 0.47347369638157966 1.784336523013302E-4 56-57 9.992284528874491E-4 3.5686730460266035E-5 0.0 0.5515384192634116 1.784336523013302E-4 58-59 9.992284528874491E-4 3.5686730460266035E-5 0.0 0.6405411250313151 1.9627701753146322E-4 60-61 9.992284528874491E-4 3.5686730460266035E-5 0.0 0.7421590900169226 2.1412038276159623E-4 62-63 0.001034915183347715 3.5686730460266035E-5 0.0 0.8426529229930317 2.1412038276159623E-4 64-65 0.001034915183347715 3.5686730460266035E-5 0.0 0.944092454326338 2.498071132218623E-4 66-67 0.0011419753747285131 3.5686730460266035E-5 0.0 1.0638214350205306 2.498071132218623E-4 68-69 0.0011419753747285131 3.5686730460266035E-5 0.0 1.2081028862713863 2.498071132218623E-4 70-71 0.0011419753747285131 3.5686730460266035E-5 0.0 1.3759375796260174 3.925540350629264E-4 72-73 0.0011598187399586462 3.5686730460266035E-5 0.0 1.5432548153889747 4.460841307533255E-4 74-75 0.0011776621051887792 3.5686730460266035E-5 0.0 1.7084486906895462 4.639274959834585E-4 76-77 0.0011776621051887792 7.137346092053207E-5 0.0 1.8913967143941 4.996142264437246E-4 78-79 0.0011776621051887792 7.137346092053207E-5 0.0 2.108479095783898 5.353009569039906E-4 80-81 0.0015166860445613066 7.137346092053207E-5 0.0 2.3546283191335835 5.531443221341236E-4 82-83 0.0016059028707119717 7.137346092053207E-5 0.0 2.616408330424865 6.958912439751877E-4 84-85 0.0016772763316325037 7.137346092053207E-5 0.0 2.8787236426730503 7.137346092053208E-4 86-87 0.001766493157783169 7.137346092053207E-5 0.0 3.1467845185253385 7.137346092053208E-4 88-89 0.0017843365230133018 7.137346092053207E-5 0.0 3.4547431590322044 7.137346092053208E-4 90-91 0.001855709983933834 7.137346092053207E-5 0.0 3.806114707143984 7.137346092053208E-4 92-93 0.001927083444854366 7.137346092053207E-5 0.0 4.1904786375662795 7.494213396655868E-4 94-95 0.001927083444854366 7.137346092053207E-5 0.0 4.577465542677404 7.494213396655868E-4 96-97 0.001927083444854366 7.137346092053207E-5 0.0 4.958724727549656 8.029514353559858E-4 98-99 0.001980613540544765 7.137346092053207E-5 0.0 5.372780017714893 8.564815310463849E-4 100-101 0.002105517097155696 7.137346092053207E-5 0.0 5.8521598679876465 8.564815310463849E-4 102-103 0.002266107384226893 7.137346092053207E-5 0.0 6.374792035578243 8.564815310463849E-4 104-105 0.0024445410365282237 7.137346092053207E-5 0.0 6.909985932290853 8.564815310463849E-4 106-107 0.0025516012279090216 7.137346092053207E-5 0.0 7.430905136819356 8.564815310463849E-4 108-109 0.0025694445931391547 7.137346092053207E-5 0.0 7.960407000023554 8.921682615066509E-4 110-111 0.0025694445931391547 7.137346092053207E-5 0.0 8.561728408279036 8.921682615066509E-4 112-113 0.0025694445931391547 7.137346092053207E-5 0.0 9.214706358875754 8.921682615066509E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8215 0.0 73.21146 1 GTATCAA 15115 0.0 50.40909 1 ATCAACG 19820 0.0 36.71805 3 TATCAAC 20730 0.0 36.40005 2 TCAACGC 19955 0.0 36.051483 4 CAACGCA 20010 0.0 35.791977 5 AACGCAG 20325 0.0 34.71046 6 GTACATG 14200 0.0 34.581 1 TACATGG 15125 0.0 31.643736 2 CGCAGAG 23575 0.0 29.991411 8 ACGCAGA 23525 0.0 29.710812 7 ACATGGG 14620 0.0 29.597622 3 GAGTACT 16670 0.0 28.004759 12-13 GCAGAGT 24555 0.0 27.753096 9 GTACTTT 17815 0.0 26.722504 14-15 CAGAGTA 24475 0.0 25.792597 10-11 AGTACTT 17490 0.0 25.297691 12-13 AGCGTAC 405 0.0 24.968943 5 CATGGGA 9890 0.0 24.82953 4 AGAGTAC 23790 0.0 24.560425 10-11 >>END_MODULE