FastQCFastQC Report
Thu 26 May 2016
SRR1364494_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364494_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2659906
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44050.1656073560494243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29320.11022945923652941No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG148550.036.469541
GTATCAA132950.036.1813351
TACATGG151300.034.332062
GGTATCA92650.032.128181
ACATGGG153800.030.7569713
ATCAACG155800.029.942043
TCAACGC157200.029.785824
CAACGCA157500.029.3497455
TATCAAC167600.028.756472
AACGCAG156450.028.6162036
CATGGGA105350.026.6899054
AACGCAT5450.026.2170896
ACGCAGA176200.025.0041397
GAGTACT55250.024.92534412-13
CGCAGAG172400.024.5906338
CGCATAG4700.024.0703688
ACGCATA4800.023.566687
CATGGGG53050.023.4693154
CTCAACG5500.022.7455334
ATGGGAG46450.022.5709655
Produced by FastQC (version 0.11.5)