##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364494_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2659906 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.181741008892796 30.0 25.0 31.0 2.0 34.0 2 25.455456320636895 30.0 25.0 31.0 2.0 34.0 3 25.421829192460184 30.0 25.0 31.0 2.0 34.0 4 28.15375280179074 35.0 26.0 35.0 2.0 37.0 5 28.728373107921858 35.0 27.0 37.0 2.0 37.0 6 28.820453429557286 35.0 28.0 37.0 2.0 37.0 7 28.59220964951393 35.0 27.0 37.0 2.0 37.0 8 28.162020387186615 35.0 26.0 37.0 2.0 37.0 9 28.683253844308783 35.0 25.0 39.0 2.0 39.0 10-11 28.75372437973372 35.0 25.5 39.0 2.0 39.0 12-13 28.58125832266253 35.0 25.0 39.0 2.0 39.0 14-15 29.154470872278946 35.5 25.0 39.5 2.0 41.0 16-17 29.419124209652523 36.0 25.5 40.0 2.0 41.0 18-19 29.394492324164837 36.0 25.0 40.0 2.0 41.0 20-21 29.18285909351684 35.5 24.5 40.0 2.0 41.0 22-23 29.240202661297054 35.0 25.0 40.0 2.0 41.0 24-25 29.26699214182757 35.5 25.0 40.0 2.0 41.0 26-27 29.37329777819216 36.0 25.5 40.0 2.0 41.0 28-29 29.308449621903932 35.0 25.0 40.0 2.0 41.0 30-31 28.955470230902897 35.0 24.5 40.0 2.0 41.0 32-33 28.93236527907377 35.0 25.0 40.0 2.0 41.0 34-35 28.62291430599427 35.0 24.0 39.5 2.0 41.0 36-37 28.364710820607947 34.0 23.5 39.0 2.0 41.0 38-39 28.19808989490606 34.0 23.0 39.0 2.0 41.0 40-41 28.238132663334717 34.5 23.5 39.0 2.0 41.0 42-43 28.07115758977949 34.0 22.5 39.0 2.0 41.0 44-45 27.70836488206726 34.0 20.5 39.0 2.0 41.0 46-47 27.396042002988075 33.0 18.5 39.0 2.0 40.5 48-49 27.101454149131584 33.0 16.5 39.0 2.0 40.0 50-51 27.082112112232537 33.0 16.5 39.0 2.0 40.0 52-53 26.874360409728766 33.0 14.5 39.0 2.0 40.0 54-55 26.741854599373063 33.0 14.0 39.0 2.0 40.0 56-57 26.327849931538935 33.0 11.5 38.0 2.0 40.0 58-59 26.101670698137454 33.0 8.5 38.0 2.0 40.0 60-61 25.694056669671784 32.0 8.0 38.0 2.0 40.0 62-63 25.63799228243404 32.0 8.0 38.0 2.0 40.0 64-65 25.46376751659645 32.0 7.5 37.5 2.0 40.0 66-67 25.236285793558118 32.0 7.0 37.0 2.0 40.0 68-69 25.060652331322988 32.0 4.0 36.5 2.0 39.5 70-71 24.93861211636802 32.0 2.0 36.0 2.0 39.0 72-73 24.897561041630794 32.0 2.0 36.0 2.0 39.0 74-75 24.620137892090924 31.5 2.0 36.0 2.0 39.0 76-77 24.369276395481645 31.0 2.0 35.0 2.0 38.5 78-79 24.05262140842571 31.0 2.0 35.0 2.0 37.0 80-81 24.02508340520304 31.0 2.0 35.0 2.0 37.0 82-83 23.821495947601157 31.0 2.0 35.0 2.0 36.5 84-85 23.62098059104344 31.0 2.0 35.0 2.0 36.0 86-87 23.41023329395851 31.0 2.0 35.0 2.0 36.0 88-89 23.184516482913306 31.0 2.0 35.0 2.0 35.5 90-91 22.9997221706331 31.0 2.0 35.0 2.0 35.0 92-93 22.882968796641684 31.0 2.0 35.0 2.0 35.0 94-95 22.789289546322316 31.0 2.0 34.0 2.0 35.0 96-97 22.651116054477114 31.0 2.0 34.0 2.0 35.0 98-99 22.538957955657082 30.0 2.0 34.0 2.0 35.0 100-101 22.47372162775677 30.0 2.0 34.0 2.0 35.0 102-103 21.417122447184223 28.5 2.0 33.5 2.0 34.5 104-105 22.107632938908367 30.0 2.0 34.0 2.0 35.0 106-107 22.191969377865234 30.0 2.0 34.0 2.0 35.0 108-109 22.113167720964576 30.0 2.0 34.0 2.0 35.0 110-111 21.990383870708214 30.0 2.0 34.0 2.0 35.0 112-113 21.94990067318168 30.0 2.0 34.0 2.0 35.0 114-115 21.875452553586477 30.0 2.0 34.0 2.0 35.0 116-117 21.687724866968985 30.0 2.0 34.0 2.0 35.0 118-119 21.51617970710243 30.0 2.0 34.0 2.0 35.0 120-121 21.351131581341598 30.0 2.0 34.0 2.0 35.0 122-123 21.064376523080142 30.0 2.0 34.0 2.0 35.0 124-125 20.107466767622615 27.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5352399033596598 1101 2 1.1761611332893906 1101 3 1.4449876488417495 1101 4 1.7007257507430111 1101 5 1.3145207941350137 1101 6 1.2437647111025214 1101 7 1.0302424337658955 1101 8 1.5261263753745027 1101 9 2.0193208206424735 1101 10-11 1.9553575610744787 1101 12-13 1.808412135400765 1101 14-15 2.077481830600181 1101 16-17 1.7203353994792963 1101 18-19 1.643791317447576 1101 20-21 2.0152142015110606 1101 22-23 2.0203329631072364 1101 24-25 1.9105721638648383 1101 26-27 1.3891610982836227 1101 28-29 1.3151978113379421 1101 30-31 1.542008425670673 1101 32-33 1.6645555523351945 1101 34-35 2.0236714081021283 1101 36-37 2.0319682387774556 1101 38-39 2.130181953275276 1101 40-41 1.8526598543365331 1101 42-43 1.8180242681566376 1101 44-45 1.9382537053695934 1101 46-47 1.9671093870250864 1101 48-49 1.8216353619724224 1101 50-51 1.7419260310365523 1101 52-53 1.6747750937101777 1101 54-55 1.7168203997657407 1101 56-57 1.9680636309518782 1101 58-59 1.9609313361995255 1101 60-61 2.2346709404390808 1101 62-63 1.9257899722724545 1101 64-65 1.9225673110006767 1101 66-67 2.07020691822515 1101 68-69 1.9154046342777669 1101 70-71 1.982255608134178 1101 72-73 1.8646151653119318 1101 74-75 1.858918259498406 1101 76-77 1.9941905974186476 1101 78-79 2.035460533588136 1101 80-81 1.8793447467194149 1101 82-83 1.9063334215119099 1101 84-85 1.8048469743197195 1101 86-87 1.740559009465919 1101 88-89 1.88092407445453 1101 90-91 1.9081953087386339 1101 92-93 1.9513881937525817 1101 94-95 1.8829321507567087 1101 96-97 1.8708297317397253 1101 98-99 1.769922257759287 1101 100-101 1.8127668350844601 1101 102-103 1.7872201402603345 1101 104-105 1.7371408758406055 1101 106-107 1.9309017313302057 1101 108-109 1.989392000305994 1101 110-111 2.0457007266444514 1101 112-113 1.878532366778476 1101 114-115 1.8661439192273086 1101 116-117 2.055346196116794 1101 118-119 2.0537258903518563 1101 120-121 1.9920926134649157 1101 122-123 2.1237154230580018 1101 124-125 2.064864588225902 1102 1 0.7960515804526338 1102 2 0.5099210026154815 1102 3 0.7386335652589899 1102 4 1.1040886862648058 1102 5 1.046739100895394 1102 6 1.1273973896767622 1102 7 0.8915854078432517 1102 8 1.1345962673874226 1102 9 1.5308014534650987 1102 10-11 1.1070666636432165 1102 12-13 1.5078083035593508 1102 14-15 1.5815994053884381 1102 16-17 1.1664148696135577 1102 18-19 1.446226150714672 1102 20-21 1.3973608360974943 1102 22-23 1.4170745743537552 1102 24-25 1.0374223295926264 1102 26-27 1.148189047826044 1102 28-29 0.8467068768055128 1102 30-31 0.8619354322599513 1102 32-33 0.7637713734103215 1102 34-35 1.1531703003938851 1102 36-37 1.314636310285895 1102 38-39 1.1699886983685204 1102 40-41 0.8649071460906264 1102 42-43 0.9101279040705634 1102 44-45 1.3929441712045616 1102 46-47 1.3523467140368624 1102 48-49 1.3341342792762028 1102 50-51 1.125878857672685 1102 52-53 1.0946434725885474 1102 54-55 0.9240732370088232 1102 56-57 1.1251221502773667 1102 58-59 1.1994465636560534 1102 60-61 1.6434852514366796 1102 62-63 1.4229300330979804 1102 64-65 1.4511244081149357 1102 66-67 1.4557813864186606 1102 68-69 1.2600851450973174 1102 70-71 1.4685258331311886 1102 72-73 1.388885103828681 1102 74-75 1.4224794175919726 1102 76-77 1.2791710938331278 1102 78-79 1.2087145093412985 1102 80-81 0.7728437696294996 1102 82-83 0.66565462540526 1102 84-85 0.8053331454519608 1102 86-87 0.8105510936248805 1102 88-89 0.5483010651102269 1102 90-91 0.5185059290737755 1102 92-93 0.4635477986923604 1102 94-95 0.6382627216514223 1102 96-97 0.6933565409193214 1102 98-99 0.7792703553635967 1102 100-101 0.734587946340703 1102 102-103 0.42304542342881746 1102 104-105 0.4523746027427009 1102 106-107 0.6793211106430412 1102 108-109 0.7730046808441422 1102 110-111 0.5736932295231476 1102 112-113 0.41806545605934176 1102 114-115 0.5041799870228516 1102 116-117 0.6959358926932069 1102 118-119 0.8105294781408006 1102 120-121 0.845581552825589 1102 122-123 1.031203749624197 1102 124-125 0.9266295532878175 1103 1 1.2779848351152552 1103 2 0.9889164058398947 1103 3 1.1233590407915592 1103 4 1.7385024556202033 1103 5 1.2682465625064907 1103 6 1.3176432181004465 1103 7 1.3172710436654071 1103 8 1.5021308106662694 1103 9 2.245656207604398 1103 10-11 1.7024606469332397 1103 12-13 1.864691972237683 1103 14-15 2.0973879433595357 1103 16-17 1.849957219635325 1103 18-19 1.5118594128039256 1103 20-21 1.9661179643099125 1103 22-23 1.790422301895191 1103 24-25 1.8162403427929554 1103 26-27 1.469751269927169 1103 28-29 1.4336196721790664 1103 30-31 1.5922271080548214 1103 32-33 1.694721221908818 1103 34-35 1.4977079386058207 1103 36-37 1.8230257848498006 1103 38-39 1.8479325210613133 1103 40-41 1.9155364061162423 1103 42-43 1.967712723364727 1103 44-45 1.7421603826126209 1103 46-47 1.969267920635641 1103 48-49 2.0311269711119486 1103 50-51 1.7557791902077362 1103 52-53 1.914918480048616 1103 54-55 1.8483248412219133 1103 56-57 2.066693998500316 1103 58-59 2.2175988867983065 1103 60-61 2.1034482729851263 1103 62-63 2.0261088923385735 1103 64-65 1.913575118003621 1103 66-67 1.9411433072556683 1103 68-69 1.9351870460760914 1103 70-71 1.9916728701065587 1103 72-73 1.9402164042699361 1103 74-75 2.0794490304633406 1103 76-77 2.07498416701614 1103 78-79 2.2143235921385696 1103 80-81 1.7788776882165394 1103 82-83 1.867258283098927 1103 84-85 1.8367770131290726 1103 86-87 1.7284084103294646 1103 88-89 1.7794605283434386 1103 90-91 1.7400619807331132 1103 92-93 1.7169244290251484 1103 94-95 1.5507194187878213 1103 96-97 1.6178714211926888 1103 98-99 1.5616486146115989 1103 100-101 1.444315418107152 1103 102-103 1.4650264981429828 1103 104-105 1.6364065462067963 1103 106-107 1.3335164091385998 1103 108-109 1.6088199256039353 1103 110-111 1.7317695381729514 1103 112-113 1.6683291537567762 1103 114-115 1.4818249300317525 1103 116-117 1.634276022914534 1103 118-119 1.7661009014698053 1103 120-121 1.6958973357979517 1103 122-123 1.7482517439718137 1103 124-125 1.706137277651564 1104 1 1.0480264720519088 1104 2 0.8680589664477694 1104 3 0.9307018188455061 1104 4 1.1018930848279993 1104 5 0.8269431986553144 1104 6 0.7238116571777127 1104 7 0.7306108098010426 1104 8 1.1360619986654577 1104 9 1.5365541449041018 1104 10-11 1.4603104692040247 1104 12-13 1.0107788785289458 1104 14-15 1.1970213929551967 1104 16-17 1.0131692382362125 1104 18-19 0.20277514065299584 1104 20-21 -4.101567238427773 1104 22-23 -0.9389636719380547 1104 24-25 0.45383293975483596 1104 26-27 0.6230134627588235 1104 28-29 0.3186296698711786 1104 30-31 1.1850844465615147 1104 32-33 0.921314377808546 1104 34-35 1.1640298862587564 1104 36-37 1.3215673665977832 1104 38-39 1.286536358884181 1104 40-41 1.3050172827672597 1104 42-43 1.379241107833682 1104 44-45 1.554207676184621 1104 46-47 1.4542719519849747 1104 48-49 1.5331879357448273 1104 50-51 1.3910330350410582 1104 52-53 1.4502285169829925 1104 54-55 1.364077504854965 1104 56-57 1.6616844401479938 1104 58-59 1.6676180740142819 1104 60-61 1.7225076465028621 1104 62-63 1.349703635556672 1104 64-65 1.4997160902083273 1104 66-67 1.4854582368623177 1104 68-69 1.3357108367323107 1104 70-71 1.5541398823329509 1104 72-73 1.414089816375263 1104 74-75 1.4173800546593007 1104 76-77 1.4141171652037272 1104 78-79 1.3764805172590684 1104 80-81 1.1114644964699423 1104 82-83 1.105644457889447 1104 84-85 1.105146826580519 1104 86-87 1.1245160225421316 1104 88-89 1.2177363257926075 1104 90-91 1.27375077249717 1104 92-93 0.9493119071903706 1104 94-95 1.0671396076985076 1104 96-97 1.1632767270613193 1104 98-99 1.0705855452157103 1104 100-101 0.6709712206060168 1104 102-103 1.1421614657660832 1104 104-105 1.265552890018384 1104 106-107 1.3297340732557394 1104 108-109 1.40106080530321 1104 110-111 1.514478832731566 1104 112-113 1.4840461006923995 1104 114-115 1.3751228015932107 1104 116-117 1.4829321991234181 1104 118-119 1.5199063298111781 1104 120-121 1.4129663644854809 1104 122-123 1.2162652040411182 1104 124-125 1.4119845647690639 1105 1 1.1550702997230573 1105 2 1.0140152585051112 1105 3 0.9869963682735978 1105 4 1.524244335719871 1105 5 1.226485142363419 1105 6 1.1326534167423112 1105 7 1.108834526552684 1105 8 1.303143706047937 1105 9 1.6267157133581875 1105 10-11 1.29845182250439 1105 12-13 1.3890963464826136 1105 14-15 1.6223540187527306 1105 16-17 1.3864183444711422 1105 18-19 1.1125066833681672 1105 20-21 1.3169337120864384 1105 22-23 1.2410834935034245 1105 24-25 1.2837371623783227 1105 26-27 1.186718637034211 1105 28-29 0.795959404848638 1105 30-31 1.0995610298895642 1105 32-33 1.2194692046275115 1105 34-35 1.001101917845407 1105 36-37 1.267242377961157 1105 38-39 1.1361682614891144 1105 40-41 1.2487785614892282 1105 42-43 1.555753248898771 1105 44-45 1.6912723127761389 1105 46-47 1.9852921687639729 1105 48-49 1.5814688567115631 1105 50-51 1.7216959354069097 1105 52-53 1.5282226909186925 1105 54-55 1.6705488298809819 1105 56-57 1.9027827892491906 1105 58-59 1.687551188585779 1105 60-61 2.0217372667901827 1105 62-63 1.8789022456226405 1105 64-65 1.5971437966817348 1105 66-67 1.9118584778362262 1105 68-69 1.3786488601720066 1105 70-71 1.5482950461253218 1105 72-73 1.5052538265321402 1105 74-75 1.7257737533649866 1105 76-77 1.6799908337660057 1105 78-79 1.5125978631005061 1105 80-81 1.5018076079913456 1105 82-83 1.51118111512411 1105 84-85 1.4490174320437141 1105 86-87 1.4931056588221416 1105 88-89 1.6603937728235856 1105 90-91 1.6207920972468948 1105 92-93 1.5581415313783396 1105 94-95 1.428974146172255 1105 96-97 1.5784124401203385 1105 98-99 1.3627996988571631 1105 100-101 1.5350378015665846 1105 102-103 1.5768229456427534 1105 104-105 1.7010302301822016 1105 106-107 1.7518824629075134 1105 108-109 1.8005274175773813 1105 110-111 1.6867344362688108 1105 112-113 1.7056080916092817 1105 114-115 1.580933154911449 1105 116-117 1.7108137957705907 1105 118-119 1.6672302896495879 1105 120-121 1.6724335293526984 1105 122-123 1.7682562455327293 1105 124-125 1.566090249813783 1106 1 -0.5752139099671822 1106 2 -0.3782572353536686 1106 3 -0.3800123291062114 1106 4 -0.6105235678489755 1106 5 -1.1460850018465756 1106 6 -0.5591911662951965 1106 7 -0.6218877491248982 1106 8 -0.18166524961381114 1106 9 -0.05645682247085304 1106 10-11 -2.572462244214833 1106 12-13 -0.8928632222392103 1106 14-15 -0.5628399814247089 1106 16-17 -0.7286466563352967 1106 18-19 -3.482907075488008 1106 20-21 -5.751023541965505 1106 22-23 -6.569653415348096 1106 24-25 -6.36344454672949 1106 26-27 -5.508860569168096 1106 28-29 -2.306695828148264 1106 30-31 -4.11387721951025 1106 32-33 -4.5262226486947235 1106 34-35 -3.9338116537264973 1106 36-37 -6.282618104659239 1106 38-39 -6.637990911778591 1106 40-41 -2.8043701262929552 1106 42-43 -1.4048325382493907 1106 44-45 -0.7111947841539248 1106 46-47 -0.5193151570710555 1106 48-49 -0.3394801999752808 1106 50-51 -0.4563552660996848 1106 52-53 -0.743896654628422 1106 54-55 -0.598411296600613 1106 56-57 -0.434905742293882 1106 58-59 -0.27450654452893275 1106 60-61 -0.11558242646628258 1106 62-63 -0.311472769836886 1106 64-65 -0.4526259199935545 1106 66-67 -0.7850760226700935 1106 68-69 -0.5223882168039289 1106 70-71 -0.31010200405336974 1106 72-73 -0.41772073223252804 1106 74-75 -0.4238884490847745 1106 76-77 -0.47397149521403037 1106 78-79 -0.4859054667541116 1106 80-81 -0.7994854389058332 1106 82-83 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-3.2666545390162476 2216 18-19 -2.80858529285889 2216 20-21 -2.396757888398401 2216 22-23 -2.64494743540034 2216 24-25 -3.2012901910701608 2216 26-27 -2.9287304544835777 2216 28-29 -3.3895063794240556 2216 30-31 -3.2358998815847393 2216 32-33 -3.621919997566998 2216 34-35 -3.097863275534703 2216 36-37 -2.897471941867231 2216 38-39 -3.245406740613131 2216 40-41 -3.443931642666289 2216 42-43 -3.5195569552357 2216 44-45 -3.347716125851914 2216 46-47 -3.4978590257504543 2216 48-49 -3.367272882553312 2216 50-51 -3.3957585547084115 2216 52-53 -3.7603286955818866 2216 54-55 -4.399210620164258 2216 56-57 -3.522534103143105 2216 58-59 -4.263044213702667 2216 60-61 -3.8107057722489337 2216 62-63 -4.098212835667319 2216 64-65 -3.898728664187743 2216 66-67 -4.6353034223109795 2216 68-69 -4.296957926888911 2216 70-71 -6.319197963303385 2216 72-73 -6.699704624705195 2216 74-75 -4.704636081605013 2216 76-77 -4.1521114514291 2216 78-79 -4.5144065172610865 2216 80-81 -4.553545539915348 2216 82-83 -4.61703847064172 2216 84-85 -4.419860325652337 2216 86-87 -4.757946702501226 2216 88-89 -4.91982745959027 2216 90-91 -4.984842541592972 2216 92-93 -4.687646102806475 2216 94-95 -7.716911675426282 2216 96-97 -9.52422724317092 2216 98-99 -6.5416103362869755 2216 100-101 -5.554356289633887 2216 102-103 -6.417400236100942 2216 104-105 -8.960604175857881 2216 106-107 -5.858190605914604 2216 108-109 -8.014594586033118 2216 110-111 -5.616515804450589 2216 112-113 -5.084456835535011 2216 114-115 -5.464036933157804 2216 116-117 -4.978269541395381 2216 118-119 -5.335876408743246 2216 120-121 -6.047587604700087 2216 122-123 -8.305070003474425 2216 124-125 -6.935012233976792 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 310217.0 3 45728.0 4 26182.0 5 21261.0 6 21108.0 7 23456.0 8 25897.0 9 27651.0 10 29471.0 11 30773.0 12 31100.0 13 32358.0 14 34463.0 15 28797.0 16 23461.0 17 20848.0 18 18901.0 19 17474.0 20 17269.0 21 18783.0 22 23107.0 23 30846.0 24 41327.0 25 52009.0 26 60900.0 27 67380.0 28 75446.0 29 84499.0 30 93403.0 31 102444.0 32 118798.0 33 141321.0 34 169926.0 35 208202.0 36 256774.0 37 257410.0 38 70713.0 39 203.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.792699303562884 19.486388914816676 14.383925641110565 22.336986140509875 2 14.895456624164344 21.130037247197432 40.32983175579317 23.64467437284505 3 18.514360209217706 27.231739526514424 28.99551880529559 25.25838145897228 4 10.78718988756902 17.273421804064128 39.111979961112404 32.82740834725445 5 13.070014806165625 39.42924037147827 33.254211631868166 14.246533190487941 6 29.72638667695311 37.78936255645136 18.392202155641073 14.092048610954459 7 27.510406609701004 34.360309310907994 20.89589329916597 17.233390780225037 8 25.533828299704798 35.34436606056596 20.177348746768608 18.94445689296064 9 26.404168883391204 17.281127042284762 20.67112706108384 35.64357701324019 10-11 23.99476633502942 28.184576225309428 27.149204407308698 20.671453032352456 12-13 25.229827083106965 25.726176882728502 27.62145371105272 21.42254232311182 14-15 22.3230534374107 26.781742092676986 26.113266856171695 24.781937613740617 16-17 21.58688841699611 30.462623723003368 26.085797326857364 21.864690533143154 18-19 21.446078661787705 28.642891818889794 28.762866594291975 21.148162925030526 20-21 22.886861947307132 27.5192584340477 28.501767058505507 21.092112560139665 22-23 22.212911360133326 27.713594038307438 28.1769916961817 21.89650290537753 24-25 21.99945590018344 27.784662063063315 28.543149239961746 21.672732796791504 26-27 21.96967987950457 28.06494826832942 28.222918043708916 21.74245380845709 28-29 21.50087902403518 28.037351378014925 28.609995307861436 21.851774290088457 30-31 21.64741519178131 27.6906369726444 28.76190461868094 21.90004321689335 32-33 21.742945004175265 27.839979606806903 28.265965738382853 22.151109650634975 34-35 22.196111801316974 27.662673834111946 28.369422493224118 21.771791871346966 36-37 22.303280354174408 27.276324577664013 28.39183260367856 22.028562464483024 38-39 21.750240164533853 27.648436721580218 28.45016327428388 22.151159839602048 40-41 21.941289440474744 27.54765538926035 28.61139855646541 21.899656613799497 42-43 21.94373680420686 27.769250582353973 27.96007843682846 22.32693417661071 44-45 21.857265022248352 27.811098656818324 28.04333065093461 22.288305669998714 46-47 22.10092605090103 27.999871418371907 27.65694947982716 22.2422530508999 48-49 22.273518400637084 27.74360817699143 27.692096845814557 22.29077657655693 50-51 21.989909620490284 27.97203901543223 27.482127702934655 22.555923661142828 52-53 22.414568780673974 28.108448039074684 27.18738044141383 22.289602738837516 54-55 22.008801008730135 27.83791359655645 27.38641399032141 22.766871404392006 56-57 22.08739118869274 28.20402243479781 27.319470280096592 22.389116096412856 58-59 22.360126416878646 27.630830376900526 27.397511896234377 22.611531309986443 60-61 22.332850589089627 27.765189990760042 27.776697630742397 22.125261789407933 62-63 22.016992314468734 27.86006024048821 27.595008860111754 22.527938584931306 64-65 21.822424618179618 27.783620139617614 27.504076598314164 22.8898786438886 66-67 21.984405769583258 28.20277199430373 27.012158611840896 22.800663624272104 68-69 21.88726775740554 27.870088902173766 27.392697952561697 22.849945387858998 70-71 21.872431650413453 27.74672886725303 27.76189928347511 22.618940198858404 72-73 22.116311130978318 27.710824122715174 27.543391946187107 22.629472800119405 74-75 21.71874532989482 28.192970296862196 27.225686363302277 22.86259800994071 76-77 21.947973625110965 27.883752417140446 27.39148858252455 22.776785375224044 78-79 22.056986467857307 27.848007256050284 27.39971225488832 22.69529402120409 80-81 21.90448046704053 28.378772635058418 27.118683576132447 22.598063321768606 82-83 22.05723697926115 28.136135258489798 27.10138277175835 22.705244990490705 84-85 21.969099412742217 27.92143829281681 27.00801182034874 23.101450474092232 86-87 22.031921281092178 27.799287388153893 27.15209416672651 23.016697164027423 88-89 22.14224752136495 27.729807160877236 27.17572836340531 22.952216954352508 90-91 21.938786731842814 27.985614526688714 27.41407939381145 22.661519347657023 92-93 22.090653589131783 27.715414734446224 27.261362903660896 22.9325687727611 94-95 22.003986407189895 27.66219945683651 27.18161613226014 23.152198003713455 96-97 22.07664845266176 27.67536838432728 27.228446029825655 23.019537133185306 98-99 21.832072376367034 27.61859502819459 27.085062383015984 23.464270212422388 100-101 21.63372924410387 27.66006602335161 27.28432581721333 23.42187891533119 102-103 22.03644811009173 27.192918807575197 27.494021371552442 23.276611710780628 104-105 21.86548151066785 27.219399422623695 27.556004880835538 23.359114185872908 106-107 21.698233797150028 27.501751014234816 27.615045300796616 23.184969887818543 108-109 21.73619469640391 27.630739720990594 27.152352488811033 23.480713093794463 110-111 21.340301144801398 27.825497678440513 27.380378592777788 23.453822583980298 112-113 21.573738799385065 27.505003220182665 27.39954364766803 23.52171433276424 114-115 21.185946190626932 27.762240049567005 27.210325907556637 23.841487852249426 116-117 21.22566935475078 27.72408669494237 27.459251068610246 23.5909928816966 118-119 21.360451402191366 27.77055501520602 27.104245768762397 23.76474781384021 120-121 21.144209490193305 27.96202900011054 27.228303494285477 23.665458015410678 122-123 21.274901072459137 27.76296867216212 27.229051329529753 23.73307892584899 124-125 21.308375671753296 27.791194661935897 26.939724083658163 23.960705582652647 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1264.0 1 1442.5 2 1775.5 3 1798.5 4 1445.5 5 1088.5 6 977.5 7 1133.0 8 1462.0 9 1645.0 10 1595.0 11 1573.0 12 1733.5 13 1976.5 14 2142.5 15 2377.0 16 2680.5 17 2963.5 18 3304.5 19 3850.5 20 4546.0 21 5512.5 22 6838.0 23 8450.0 24 10315.5 25 12899.5 26 16760.0 27 21019.0 28 24978.5 29 29449.0 30 34922.5 31 41110.0 32 47463.0 33 54416.5 34 61916.5 35 67025.0 36 71623.5 37 77345.0 38 80232.0 39 80555.0 40 81013.5 41 84579.0 42 88378.0 43 90337.5 44 91848.0 45 92440.5 46 92026.5 47 92483.0 48 96844.0 49 97999.0 50 93638.5 51 88793.0 52 84506.0 53 81808.5 54 78951.0 55 73367.5 56 66466.0 57 60404.0 58 55711.0 59 50466.0 60 44359.5 61 37759.5 62 31109.0 63 24987.0 64 19915.5 65 15785.0 66 11949.5 67 9435.0 68 6998.5 69 4678.0 70 3427.5 71 2715.5 72 2250.0 73 1894.5 74 1499.0 75 1195.0 76 889.5 77 596.5 78 397.0 79 286.5 80 188.0 81 128.5 82 97.5 83 69.5 84 54.5 85 42.5 86 32.0 87 26.5 88 24.5 89 18.5 90 13.5 91 9.5 92 5.5 93 5.5 94 3.5 95 2.5 96 4.0 97 4.5 98 4.0 99 2.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11105655613393858 2 0.0343621165559986 3 0.04661818876306155 4 0.05545308743993209 5 0.05842311720790133 6 0.003533959470748214 7 0.0021805281840786856 8 0.0023309094381530776 9 0.007556658017238203 10-11 0.06505869004393389 12-13 0.007105514255015026 14-15 0.013007978477434916 16-17 0.024023405338384138 18-19 0.022444402170603023 20-21 0.1427494054301167 22-23 0.01981273022430116 24-25 0.017575809069944576 26-27 0.008270968974091564 28-29 0.005188153265566528 30-31 0.002500088348986769 32-33 0.007331086136126616 34-35 0.017820178607815464 36-37 0.003251994619358729 38-39 0.010188329963540065 40-41 0.03558396424535303 42-43 0.016579533261701727 44-45 0.11579356563728192 46-47 0.004398651681675969 48-49 0.011165808115023615 50-51 0.012895192536879123 52-53 0.014417802734382344 54-55 0.012199679236785058 56-57 0.007462669733441708 58-59 0.01695548639688771 60-61 0.030189036755434214 62-63 0.00875970804983334 64-65 0.00800780177946138 66-67 0.01009434167974357 68-69 0.009060470557982125 70-71 0.004661818876306155 72-73 8.646922109277546E-4 74-75 0.0013346336299102299 76-77 0.009586804947242495 78-79 0.0228579506193076 80-81 0.003533959470748214 82-83 0.005244546235844425 84-85 0.00323319696259943 86-87 0.004248270427601578 88-89 0.006616775179273253 90-91 0.005244546235844425 92-93 0.002913636797691347 94-95 0.004417449338435268 96-97 0.0018045750488927053 98-99 0.0032895899328773274 100-101 0.003364780559914523 102-103 5.639297027789703E-4 104-105 0.004342258711398072 106-107 7.143109568533625E-4 108-109 0.0011842523758358377 110-111 0.0021053375570414894 112-113 0.004473842308713165 114-115 0.0027068625733390576 116-117 0.005376129833159517 118-119 0.003947507919452793 120-121 0.007218300195570821 122-123 0.004924986070936342 124-125 0.05879907034308732 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2659906.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.40358742124307 #Duplication Level Percentage of deduplicated Percentage of total 1 81.93719272112128 48.67363190860369 2 9.189812818879556 10.918156983423437 3 3.455611783601363 6.158272100431237 4 1.7387446914783915 4.131506891338358 5 0.9817345694608404 2.915927766071173 6 0.621465165889241 2.215035618669531 7 0.396875697347185 1.6503088127911212 8 0.2825380193909729 1.3427017547773221 9 0.20493882436305286 1.0956691232151652 >10 1.0635504979887944 11.360965001450046 >50 0.07969125110782777 3.30415321063758 >100 0.04569761620727624 4.902326714465999 >500 0.0013414644775771435 0.5056987938153954 >1k 8.048786865396325E-4 0.8256453203098495 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4405 0.1656073560494243 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2932 0.11022945923652941 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.759531351859803E-5 0.0 3 0.0 0.0 0.0 3.759531351859803E-5 0.0 4 0.0 0.0 0.0 3.759531351859803E-5 0.0 5 0.0 0.0 0.0 3.759531351859803E-5 0.0 6 0.0 0.0 0.0 3.759531351859803E-5 0.0 7 0.0 0.0 0.0 3.759531351859803E-5 0.0 8 0.0 0.0 0.0 3.759531351859803E-5 0.0 9 0.0 0.0 0.0 7.519062703719605E-5 0.0 10-11 0.0 0.0 0.0 9.398828379649507E-5 0.0 12-13 0.0 0.0 0.0 1.315835973150931E-4 0.0 14-15 0.0 0.0 0.0 1.503812540743921E-4 0.0 16-17 0.0 0.0 0.0 2.631671946301862E-4 0.0 18-19 0.0 0.0 0.0 2.8196485138948517E-4 0.0 20-21 0.0 0.0 0.0 5.075367325010733E-4 0.0 22-23 0.0 0.0 0.0 9.962758082428477E-4 0.0 24-25 0.0 0.0 0.0 0.0013910266001881271 0.0 26-27 0.0 0.0 0.0 0.0018985633326892002 0.0 28-29 0.0 0.0 0.0 0.003007625081487842 0.0 30-31 1.8797656759299014E-5 0.0 0.0 0.005940059535938488 0.0 32-33 3.759531351859803E-5 0.0 3.759531351859803E-5 0.012331262834100153 3.759531351859803E-5 34-35 3.759531351859803E-5 0.0 3.759531351859803E-5 0.02018868335948714 3.759531351859803E-5 36-37 3.759531351859803E-5 0.0 3.759531351859803E-5 0.03458768843711019 3.759531351859803E-5 38-39 3.759531351859803E-5 0.0 3.759531351859803E-5 0.0589306539404024 3.759531351859803E-5 40-41 3.759531351859803E-5 0.0 3.759531351859803E-5 0.09002197822028296 3.759531351859803E-5 42-43 3.759531351859803E-5 3.759531351859803E-5 3.759531351859803E-5 0.1218464111137762 3.759531351859803E-5 44-45 3.759531351859803E-5 9.398828379649507E-5 3.759531351859803E-5 0.15868981836200227 3.759531351859803E-5 46-47 3.759531351859803E-5 1.1278594055579408E-4 3.759531351859803E-5 0.20410495709246868 7.519062703719605E-5 48-49 3.759531351859803E-5 1.503812540743921E-4 3.759531351859803E-5 0.2563436452265606 7.519062703719605E-5 50-51 3.759531351859803E-5 1.503812540743921E-4 3.759531351859803E-5 0.31711646952937433 7.519062703719605E-5 52-53 3.759531351859803E-5 1.503812540743921E-4 3.759531351859803E-5 0.3757839562751466 7.519062703719605E-5 54-55 3.759531351859803E-5 1.503812540743921E-4 3.759531351859803E-5 0.43759065169972167 7.519062703719605E-5 56-57 3.759531351859803E-5 1.503812540743921E-4 3.759531351859803E-5 0.5128000763936771 7.519062703719605E-5 58-59 3.759531351859803E-5 1.691789108336911E-4 3.759531351859803E-5 0.6006039311163627 7.519062703719605E-5 60-61 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 0.6995359986405535 7.519062703719605E-5 62-63 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 0.803167480354569 7.519062703719605E-5 64-65 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 0.9047876127953394 1.1278594055579408E-4 66-67 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 1.0241151379033695 1.1278594055579408E-4 68-69 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 1.16703372224432 1.1278594055579408E-4 70-71 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 1.3369645393483829 1.1278594055579408E-4 72-73 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 1.5097901955933781 1.1278594055579408E-4 74-75 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 1.6837625089006905 1.1278594055579408E-4 76-77 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 1.87094957490979 1.1278594055579408E-4 78-79 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 2.0937582004777613 1.1278594055579408E-4 80-81 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 2.350609382436823 1.1278594055579408E-4 82-83 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 2.6242656695386977 1.1278594055579408E-4 84-85 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 2.8913051814613 1.1278594055579408E-4 86-87 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 3.164980266219934 1.1278594055579408E-4 88-89 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 3.4781680254866147 1.1278594055579408E-4 90-91 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 3.8389138563543224 1.1278594055579408E-4 92-93 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 4.223551508963098 1.1278594055579408E-4 94-95 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 4.606140216985112 1.1278594055579408E-4 96-97 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 4.989875582069441 1.1278594055579408E-4 98-99 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 5.402540540906333 1.1278594055579408E-4 100-101 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 5.873309056786217 1.1278594055579408E-4 102-103 3.759531351859803E-5 2.2557188111158816E-4 3.759531351859803E-5 6.3911093098778675 1.1278594055579408E-4 104-105 3.759531351859803E-5 2.443695378708872E-4 3.759531351859803E-5 6.908984753596556 1.1278594055579408E-4 106-107 3.759531351859803E-5 2.8196485138948517E-4 3.759531351859803E-5 7.4159011634245715 1.1278594055579408E-4 108-109 3.759531351859803E-5 3.383578216673822E-4 3.759531351859803E-5 7.9418783972065174 1.1278594055579408E-4 110-111 3.759531351859803E-5 3.383578216673822E-4 3.759531351859803E-5 8.53095936472943 1.503812540743921E-4 112-113 3.759531351859803E-5 3.383578216673822E-4 3.759531351859803E-5 9.168989430453557 1.503812540743921E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14855 0.0 36.46954 1 GTATCAA 13295 0.0 36.181335 1 TACATGG 15130 0.0 34.33206 2 GGTATCA 9265 0.0 32.12818 1 ACATGGG 15380 0.0 30.756971 3 ATCAACG 15580 0.0 29.94204 3 TCAACGC 15720 0.0 29.78582 4 CAACGCA 15750 0.0 29.349745 5 TATCAAC 16760 0.0 28.75647 2 AACGCAG 15645 0.0 28.616203 6 CATGGGA 10535 0.0 26.689905 4 AACGCAT 545 0.0 26.217089 6 ACGCAGA 17620 0.0 25.004139 7 GAGTACT 5525 0.0 24.925344 12-13 CGCAGAG 17240 0.0 24.590633 8 CGCATAG 470 0.0 24.070368 8 ACGCATA 480 0.0 23.56668 7 CATGGGG 5305 0.0 23.469315 4 CTCAACG 550 0.0 22.745533 4 ATGGGAG 4645 0.0 22.570965 5 >>END_MODULE