FastQCFastQC Report
Thu 26 May 2016
SRR1364494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2659906
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109150.41035284705549746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98710.3711033397420811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58700.2206844903541704No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27010.10154494181373326No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126650.042.2540781
GTATCAA187300.039.242291
GTACATG146750.031.710121
TATCAAC235700.030.5672932
ATCAACG232100.030.1427753
TCAACGC232600.030.1292214
TACATGG151800.030.03172
CAACGCA234000.029.8982725
AACGCAG238550.029.1534356
ACATGGG155350.027.6959463
GAGTACT142750.027.16359912-13
CGCAGAG273450.025.467048
AGTACTT149200.025.4312512-13
ACGCAGA271800.025.2368137
GTACTTT155900.025.2157114-15
AGAGTAC221900.024.77795410-11
GCAGAGT277500.023.93819
CATGGGG51900.023.7680344
CATGGGA103850.023.5270564
AGCGTAC3005.5606506E-921.8135385
Produced by FastQC (version 0.11.5)