##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364494_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2659906 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.18448396296711 31.0 28.0 33.0 25.0 34.0 2 29.31074368793484 31.0 28.0 33.0 25.0 34.0 3 29.378575032350767 31.0 28.0 34.0 25.0 34.0 4 32.80747101589304 35.0 32.0 37.0 26.0 37.0 5 31.97587320754944 35.0 32.0 37.0 22.0 37.0 6 32.05841935767655 35.0 32.0 37.0 23.0 37.0 7 31.498955978143588 35.0 32.0 37.0 17.0 37.0 8 31.621768212861657 35.0 32.0 37.0 18.0 37.0 9 32.18240832570775 37.0 31.0 39.0 15.0 39.0 10-11 32.37313010309387 37.0 31.5 39.0 17.0 39.0 12-13 32.14614726986593 37.0 30.5 39.0 15.0 39.0 14-15 33.06597469985782 38.0 31.5 40.0 16.5 41.0 16-17 33.148809206039616 38.0 32.0 40.0 17.0 41.0 18-19 33.0737909535149 38.0 31.0 40.0 16.0 41.0 20-21 33.00671884645548 38.0 31.0 40.0 14.0 41.0 22-23 32.97195727969334 38.0 31.0 40.0 14.5 41.0 24-25 32.818843222279284 38.0 31.0 40.0 13.0 41.0 26-27 32.84502591444961 37.5 31.0 40.0 14.0 41.0 28-29 32.702961683608365 37.0 31.0 40.0 10.5 41.0 30-31 32.15267795177725 37.0 30.0 40.0 9.0 41.0 32-33 32.04092757413232 37.0 30.0 40.0 9.0 41.0 34-35 31.844038661516613 37.0 30.0 40.0 8.0 41.0 36-37 31.742582444642782 36.0 30.0 40.0 7.5 41.0 38-39 31.514311407997123 36.0 30.0 40.0 2.0 41.0 40-41 31.17090735537271 36.0 29.5 40.0 2.0 41.0 42-43 31.022958894036105 36.0 29.0 40.0 2.0 41.0 44-45 30.86960742221718 36.0 29.0 40.0 2.0 41.0 46-47 30.595159189836032 36.0 28.5 40.0 2.0 41.0 48-49 30.21999724802305 36.0 28.0 40.0 2.0 41.0 50-51 29.875124722452597 35.0 27.0 40.0 2.0 41.0 52-53 29.75147298438366 35.0 27.0 40.0 2.0 41.0 54-55 29.28149904545499 35.0 26.0 39.5 2.0 41.0 56-57 29.297679504463694 35.0 26.0 39.0 2.0 41.0 58-59 29.080815825822416 35.0 26.0 39.0 2.0 41.0 60-61 28.814857367140043 34.5 26.0 39.0 2.0 41.0 62-63 28.566791270067437 34.0 25.5 39.0 2.0 40.5 64-65 28.098413816127337 34.0 23.0 38.0 2.0 40.0 66-67 27.752031838719112 34.0 22.5 38.0 2.0 40.0 68-69 27.556273417180908 34.0 22.5 37.5 2.0 40.0 70-71 27.60988264246932 34.0 23.5 37.0 2.0 40.0 72-73 27.406620196352804 34.0 23.5 37.0 2.0 39.0 74-75 27.04898387386622 34.0 22.5 36.0 2.0 39.0 76-77 26.943806848813452 33.0 23.0 36.0 2.0 39.0 78-79 26.533017520168006 33.0 22.0 35.5 2.0 37.5 80-81 26.311531497729618 33.0 21.5 35.0 2.0 37.0 82-83 26.152294479579353 33.0 21.0 35.0 2.0 37.0 84-85 25.74674800538064 33.0 19.5 35.0 2.0 36.0 86-87 25.61729399459981 33.0 20.0 35.0 2.0 36.0 88-89 25.539631287722198 33.0 20.0 35.0 2.0 36.0 90-91 25.363178811582067 33.0 19.5 35.0 2.0 35.0 92-93 25.386219475425072 33.0 20.0 35.0 2.0 35.0 94-95 25.362752480726762 33.0 20.0 35.0 2.0 35.0 96-97 25.231875111376116 33.0 19.0 35.0 2.0 35.0 98-99 25.121590575005282 33.0 18.0 35.0 2.0 35.0 100-101 25.03131313662964 33.0 18.0 35.0 2.0 35.0 102-103 23.872474252849536 30.5 14.5 34.0 2.0 35.0 104-105 24.802645469426363 32.0 17.5 35.0 2.0 35.0 106-107 24.924866893792487 32.5 17.0 35.0 2.0 35.0 108-109 24.974580116740967 33.0 17.0 35.0 2.0 35.0 110-111 24.913962561082986 33.0 15.0 35.0 2.0 35.0 112-113 24.844041857118256 33.0 11.5 35.0 2.0 35.0 114-115 24.776501500428964 33.0 8.5 35.0 2.0 35.0 116-117 24.68700190909002 33.0 4.5 35.0 2.0 35.0 118-119 24.560666805518693 33.0 2.0 35.0 2.0 35.0 120-121 24.395880531116514 33.0 2.0 35.0 2.0 35.0 122-123 24.101639494027232 32.5 2.0 35.0 2.0 35.0 124-125 23.311694097460588 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7090971653023246 1101 2 0.6210291298699033 1101 3 0.6535392636555528 1101 4 0.6323512343867179 1101 5 0.7823737100090931 1101 6 0.8299052195851431 1101 7 1.1777406724230168 1101 8 1.0908153662169617 1101 9 1.734028849970521 1101 10-11 1.4093419260432398 1101 12-13 1.6238563824402732 1101 14-15 1.537011239287942 1101 16-17 1.3545303477474775 1101 18-19 1.5212847185676068 1101 20-21 1.7269205743246658 1101 22-23 1.7769499408915692 1101 24-25 1.8252416525237791 1101 26-27 1.607893221385062 1101 28-29 1.5719010100004311 1101 30-31 2.0323466293328067 1101 32-33 1.886229343738247 1101 34-35 1.9418718318312784 1101 36-37 1.6959594945171723 1101 38-39 1.9925912807761108 1101 40-41 2.1644986870897114 1101 42-43 2.013027841912848 1101 44-45 1.862218455872224 1101 46-47 1.630086569315786 1101 48-49 1.670987355616134 1101 50-51 1.8614066894567252 1101 52-53 1.703813827903943 1101 54-55 1.993116901423324 1101 56-57 1.7155494268354587 1101 58-59 1.8082490762132046 1101 60-61 1.8186290501016629 1101 62-63 1.8636133561258035 1101 64-65 2.0115437582965043 1101 66-67 2.1816068374213167 1101 68-69 2.079319932734947 1101 70-71 1.711785698200245 1101 72-73 1.8530044222953705 1101 74-75 1.9716910141585196 1101 76-77 1.7561170888630322 1101 78-79 1.8490861236286023 1101 80-81 1.9293721694237789 1101 82-83 1.840607070733757 1101 84-85 2.1138805212667933 1101 86-87 2.006465858670957 1101 88-89 1.8684247118250568 1101 90-91 1.8222492338395817 1101 92-93 1.837507707731369 1101 94-95 1.7490863767450193 1101 96-97 1.7204836393730751 1101 98-99 1.8342699732145356 1101 100-101 1.8966384476872804 1101 102-103 1.717804297430554 1101 104-105 1.736662659312472 1101 106-107 1.8913164065749157 1101 108-109 1.888606918886481 1101 110-111 1.8987003426248208 1101 112-113 1.7057525526338928 1101 114-115 1.7893838794659516 1101 116-117 1.8005137694485143 1101 118-119 1.7620043813800663 1101 120-121 1.7207891069619983 1101 122-123 1.7634102125158329 1101 124-125 1.664146010048821 1102 1 0.36412258955712673 1102 2 -1.825504320000917 1102 3 -0.04001381879795929 1102 4 0.37511349699254737 1102 5 0.8150180432581315 1102 6 0.326879691576778 1102 7 1.122769521934952 1102 8 0.8408132818819887 1102 9 1.547152297719947 1102 10-11 1.2749667899824857 1102 12-13 1.3813890532330007 1102 14-15 1.4191347145969146 1102 16-17 1.3554341964488472 1102 18-19 1.4081728099281818 1102 20-21 1.5690084329808087 1102 22-23 1.5856259678434128 1102 24-25 1.8120612445314137 1102 26-27 1.5048205121386786 1102 28-29 1.5583913060315808 1102 30-31 1.861945123887125 1102 32-33 1.529734185416796 1102 34-35 1.8429290586374947 1102 36-37 1.2741779566763007 1102 38-39 1.1792701975425928 1102 40-41 1.3345716202863116 1102 42-43 1.1663479617204224 1102 44-45 1.2100205478034418 1102 46-47 0.9231142777701251 1102 48-49 1.0558532078173286 1102 50-51 1.2907268616042664 1102 52-53 1.0059342739794204 1102 54-55 1.372500721055168 1102 56-57 1.0012502925755697 1102 58-59 0.988396034566339 1102 60-61 0.952621623847751 1102 62-63 1.4402700145107126 1102 64-65 1.5422478651774938 1102 66-67 1.6068557533355374 1102 68-69 1.5599653482170197 1102 70-71 0.970392756823788 1102 72-73 1.2533305686101315 1102 74-75 1.5259491483915468 1102 76-77 1.3975532280817866 1102 78-79 1.2711570945396211 1102 80-81 1.2442792289273754 1102 82-83 0.8729430671524057 1102 84-85 0.9526388863978141 1102 86-87 1.1040585617847682 1102 88-89 1.1372859458347335 1102 90-91 0.8969313901257969 1102 92-93 0.9082575634490695 1102 94-95 0.9207776513019574 1102 96-97 0.9883258362250835 1102 98-99 0.7137080145047463 1102 100-101 0.5683527078271098 1102 102-103 0.3886008422516305 1102 104-105 0.8579651359192866 1102 106-107 0.9973780232221756 1102 108-109 0.8758038044731649 1102 110-111 0.7487026817118476 1102 112-113 0.7670864830134008 1102 114-115 1.0982893280102068 1102 116-117 0.7172948718949712 1102 118-119 0.2905383192168607 1102 120-121 0.5034739110484061 1102 122-123 0.8753964065757565 1102 124-125 0.8914702403965507 1103 1 0.6920776743239365 1103 2 0.6173211282037485 1103 3 0.5519827285461645 1103 4 0.5589632932022184 1103 5 0.7506556828134912 1103 6 0.8152370100551423 1103 7 1.1100456173352207 1103 8 0.8731170858226811 1103 9 1.561868539689435 1103 10-11 1.2990665233973004 1103 12-13 1.2419989185306832 1103 14-15 1.0926755984844974 1103 16-17 1.0600656451493506 1103 18-19 1.151931344082449 1103 20-21 1.3591105034868036 1103 22-23 1.2668163966914463 1103 24-25 1.610485996459012 1103 26-27 1.1227435286367893 1103 28-29 1.2314972929943266 1103 30-31 1.7964538226689015 1103 32-33 1.5486511548237978 1103 34-35 1.5663788718057106 1103 36-37 1.6497840310197098 1103 38-39 1.6459801697383796 1103 40-41 1.8290760596603874 1103 42-43 1.8478688959474177 1103 44-45 1.6425601137986447 1103 46-47 1.6700886531059425 1103 48-49 1.714352316767151 1103 50-51 1.8446952262551584 1103 52-53 1.6230688580611101 1103 54-55 1.891809144219991 1103 56-57 1.690207626907494 1103 58-59 1.7832678391309429 1103 60-61 1.7350357658722437 1103 62-63 1.8074598621391829 1103 64-65 1.85851804654952 1103 66-67 2.1327405821848693 1103 68-69 2.236440710445507 1103 70-71 1.9518201341260628 1103 72-73 2.0705655721248384 1103 74-75 2.1543572924674628 1103 76-77 1.9651811247363682 1103 78-79 2.0697874890890695 1103 80-81 2.02122124753652 1103 82-83 1.8785196402704827 1103 84-85 2.0815790493381883 1103 86-87 1.9858364736851648 1103 88-89 1.8661572278018816 1103 90-91 1.8981549230160262 1103 92-93 1.6299581348808339 1103 94-95 1.6045937746954415 1103 96-97 1.5541806177562556 1103 98-99 1.6321730121357305 1103 100-101 1.6036000625773212 1103 102-103 1.5036724574691611 1103 104-105 1.4976452406760572 1103 106-107 1.4619363082790215 1103 108-109 1.3777227478981224 1103 110-111 1.462692294549484 1103 112-113 1.4818622892287259 1103 114-115 1.333165653688333 1103 116-117 1.3926381819583895 1103 118-119 1.438304174388044 1103 120-121 1.4124750153888321 1103 122-123 1.616317991426687 1103 124-125 1.4937210907805536 1104 1 0.6670912816534269 1104 2 0.5481039584794694 1104 3 0.5505945233007203 1104 4 0.5591950584993128 1104 5 0.9448273795139137 1104 6 0.8342661179431659 1104 7 1.2702210386607184 1104 8 1.0846945570208426 1104 9 1.740396377153239 1104 10-11 1.1526137175754485 1104 12-13 1.172074764307233 1104 14-15 1.2020734290680224 1104 16-17 1.0240450439101494 1104 18-19 1.0434382280216212 1104 20-21 1.4010134847869296 1104 22-23 1.3259412806528559 1104 24-25 1.5736106894974569 1104 26-27 1.4226024246301492 1104 28-29 1.3252723634988683 1104 30-31 1.749118106595219 1104 32-33 1.6615873521101427 1104 34-35 1.5657740744864412 1104 36-37 1.3647169462751343 1104 38-39 1.577270871383007 1104 40-41 1.7191129452186047 1104 42-43 1.812038621042749 1104 44-45 1.5494707659171993 1104 46-47 1.4566437437387627 1104 48-49 1.222008246457598 1104 50-51 1.5780053993306247 1104 52-53 1.474514082559672 1104 54-55 1.7197459205127856 1104 56-57 1.6950741828713802 1104 58-59 1.7618998706353999 1104 60-61 1.7625906962273952 1104 62-63 1.727125904238239 1104 64-65 1.8989055767846352 1104 66-67 1.8297422779822057 1104 68-69 2.0210028798728246 1104 70-71 1.767493987794996 1104 72-73 1.8971176144988888 1104 74-75 1.9683471187682429 1104 76-77 1.8279387520954096 1104 78-79 1.8622955795854743 1104 80-81 1.7318495549582948 1104 82-83 1.8267206387650674 1104 84-85 2.0422095941385052 1104 86-87 1.9435497751853497 1104 88-89 1.7633624572470872 1104 90-91 1.855332528294177 1104 92-93 1.5642378115376019 1104 94-95 1.641415682889157 1104 96-97 1.7060426184174382 1104 98-99 1.755336659968549 1104 100-101 1.69672843576447 1104 102-103 1.5737051177719117 1104 104-105 1.5534123482825777 1104 106-107 1.6990584591130506 1104 108-109 1.5560906060163262 1104 110-111 1.6030900760462785 1104 112-113 1.578877463536788 1104 114-115 1.4976052744350454 1104 116-117 1.5808546549765552 1104 118-119 1.542680758011354 1104 120-121 1.438483144259667 1104 122-123 1.5485767593063926 1104 124-125 1.382296710514069 1105 1 0.43508200459140056 1105 2 0.49789519207842403 1105 3 0.28741878191859627 1105 4 0.48622796433149773 1105 5 0.45860477011538947 1105 6 0.5556552068310054 1105 7 0.9313598787462531 1105 8 0.8002268639396881 1105 9 1.3250677711992083 1105 10-11 1.0802707284913353 1105 12-13 1.116470197721668 1105 14-15 1.0471205972182887 1105 16-17 1.0598773438403128 1105 18-19 1.24160017895678 1105 20-21 1.3530652031616768 1105 22-23 1.274751336546018 1105 24-25 1.5054290239928676 1105 26-27 1.2200995573765354 1105 28-29 1.172333408235616 1105 30-31 1.5172662914625192 1105 32-33 1.422543890667324 1105 34-35 1.4702567688434343 1105 36-37 1.2534688336241722 1105 38-39 1.3702158293236337 1105 40-41 1.4879550406730928 1105 42-43 1.3872535228518394 1105 44-45 1.2507947951006102 1105 46-47 1.1299234592942007 1105 48-49 1.0990612785952152 1105 50-51 1.2694122524595208 1105 52-53 1.3360884688969747 1105 54-55 1.5483495720692844 1105 56-57 1.2998748891069596 1105 58-59 1.2794496297427607 1105 60-61 1.163975131415004 1105 62-63 1.0521563916632282 1105 64-65 1.4622006403409635 1105 66-67 1.6560253367255555 1105 68-69 1.5786312643020537 1105 70-71 1.3938223645854428 1105 72-73 1.5749275636657458 1105 74-75 1.5601908725436147 1105 76-77 1.4557362679188053 1105 78-79 1.6084925746711853 1105 80-81 1.5266797490755621 1105 82-83 1.4359803645380609 1105 84-85 1.751370394921672 1105 86-87 1.6325157110748023 1105 88-89 1.452433629605732 1105 90-91 1.519816371177889 1105 92-93 1.3242581382934517 1105 94-95 1.2102445865391402 1105 96-97 1.3147950962825625 1105 98-99 1.390173940278956 1105 100-101 1.3481341891248562 1105 102-103 1.2943618854385015 1105 104-105 0.9711305940922159 1105 106-107 1.1119827706606742 1105 108-109 1.1099788836217321 1105 110-111 1.2458884072152898 1105 112-113 1.3125190841946655 1105 114-115 1.2104532522966522 1105 116-117 1.1602795573111493 1105 118-119 1.258051973223047 1105 120-121 1.2275239510888518 1105 122-123 1.3202732687061633 1105 124-125 1.1916924175162364 1106 1 0.2594357964175771 1106 2 0.1411840322146496 1106 3 0.2482424079235237 1106 4 0.1421921774377708 1106 5 0.25112056759349954 1106 6 0.4118029651610655 1106 7 0.6689201874898494 1106 8 0.23824030548564323 1106 9 0.9270632502697254 1106 10-11 0.5810697011159718 1106 12-13 0.5551411254281717 1106 14-15 0.5545603072805605 1106 16-17 0.6093172941751419 1106 18-19 0.6540430118985441 1106 20-21 0.43017438579913403 1106 22-23 0.4952662875390459 1106 24-25 0.3160596244065417 1106 26-27 0.26999770703448434 1106 28-29 0.38603986242994637 1106 30-31 0.7727827701553593 1106 32-33 0.514136464077108 1106 34-35 0.5924747456113337 1106 36-37 0.6604091263363223 1106 38-39 0.6622982069688135 1106 40-41 0.9193470484467774 1106 42-43 1.0224615389284146 1106 44-45 0.9340088697625397 1106 46-47 0.7427294334522898 1106 48-49 0.5836817884465013 1106 50-51 0.9099983028897682 1106 52-53 0.7872345424429454 1106 54-55 1.1839973687854233 1106 56-57 0.8160558408916003 1106 58-59 0.7210354109172918 1106 60-61 0.965230455963269 1106 62-63 1.0791308579473018 1106 64-65 1.158006346570815 1106 66-67 1.3067148924138081 1106 68-69 1.1563968268728075 1106 70-71 1.0657476668122534 1106 72-73 0.9864858019944407 1106 74-75 1.2252345563511255 1106 76-77 0.8520635271058588 1106 78-79 0.8299314466556957 1106 80-81 1.0045139419843352 1106 82-83 0.8237775607829931 1106 84-85 1.161946414083296 1106 86-87 1.2209320295195738 1106 88-89 0.918462718514764 1106 90-91 1.012415821559948 1106 92-93 0.9304383090816621 1106 94-95 0.8885773781898685 1106 96-97 0.8173989339618579 1106 98-99 0.7667949780218812 1106 100-101 0.4168791875949438 1106 102-103 0.1924953924642594 1106 104-105 0.4706219508069154 1106 106-107 0.42285082275158814 1106 108-109 0.4457808552284206 1106 110-111 0.4119648647747738 1106 112-113 0.12717286714889653 1106 114-115 0.28983385132755046 1106 116-117 0.6507249066986454 1106 118-119 0.3564199119256699 1106 120-121 0.40821931225766406 1106 122-123 0.6119095602165103 1106 124-125 0.608759556881175 1107 1 0.4573620398495635 1107 2 0.5116087299878522 1107 3 0.3438028662909183 1107 4 0.39217532325331206 1107 5 0.3432711670655699 1107 6 0.7443275559420144 1107 7 0.9772283317970896 1107 8 0.9201331549387497 1107 9 1.6431096515830745 1107 10-11 1.0394300961625618 1107 12-13 1.3615992669955688 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-4.432128127964216 2216 92-93 -3.9190368437661256 2216 94-95 -3.6583081776057362 2216 96-97 -3.8119966437874275 2216 98-99 -3.7773376341958738 2216 100-101 -4.182417178010823 2216 102-103 -4.198269164685335 2216 104-105 -3.9414704520123536 2216 106-107 -4.052917120695916 2216 108-109 -4.09230676402948 2216 110-111 -3.8011078388355557 2216 112-113 -3.8457562219680987 2216 114-115 -3.6467570474730664 2216 116-117 -3.6229537959518936 2216 118-119 -3.767629647602302 2216 120-121 -3.8708340895073476 2216 122-123 -3.8871447090993456 2216 124-125 -3.7871661626134596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 115303.0 3 39954.0 4 19810.0 5 16605.0 6 18223.0 7 22198.0 8 24649.0 9 26094.0 10 28053.0 11 30651.0 12 34693.0 13 38687.0 14 41638.0 15 33544.0 16 22465.0 17 18998.0 18 16862.0 19 15419.0 20 14822.0 21 15701.0 22 18986.0 23 25317.0 24 34192.0 25 46211.0 26 56431.0 27 63671.0 28 70011.0 29 80374.0 30 90046.0 31 102149.0 32 119349.0 33 141906.0 34 172585.0 35 218603.0 36 299752.0 37 384748.0 38 140543.0 39 663.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63498876191365 20.203790991164592 14.577935714834123 22.583284532087635 2 15.77435241926388 21.63032444828753 38.76781081995564 23.827512312492953 3 18.855290374923023 26.842001183500468 30.02158722902238 24.281121212554126 4 12.378513791883856 18.2033356045788 37.262700989215915 32.15544961432143 5 12.67913227008774 39.37443654023864 33.508966106321054 14.437465083352569 6 29.912669747731403 38.35703783183071 17.412401880820465 14.317890539617423 7 26.692219249737946 34.235609559238625 20.983507413596424 18.088663777426998 8 26.346043807563124 35.058945692065805 19.42493456535682 19.170075935014243 9 26.355818589077963 16.14884135003267 20.550613442730683 36.94472661815868 10-11 24.710603253183923 27.64037112109859 26.737907801705074 20.911117824012415 12-13 26.02084147120425 25.06341430037118 27.696080550729747 21.21966367769482 14-15 22.846588175937384 25.928201152717058 26.818144137190664 24.407066534154897 16-17 21.400599221816215 29.44905655837337 27.0982256823207 22.05211853748971 18-19 21.14154687203051 27.04991175302351 30.776962510684285 21.031578864261693 20-21 22.39613342038188 25.997506972750205 30.746628012400095 20.859731594467817 22-23 21.68873867184489 26.017940827601503 30.730829312124158 21.562491188429444 24-25 21.6028682184678 26.287089915122348 30.8694418820879 21.24059998432195 26-27 21.498636167960942 26.532370097583076 30.85563306808633 21.113360666369648 28-29 21.013023486640023 26.505558667724387 31.258285366247968 21.223132479387623 30-31 21.117958947818384 26.166808840839995 31.13670789279077 21.578524318550855 32-33 21.173838915299605 26.348497408742045 30.858320127974498 21.619343547983856 34-35 21.784569089680733 26.237701931937252 30.707865688122233 21.26986329025978 36-37 21.92531553697821 25.92554787911667 30.61436637610741 21.53477020779771 38-39 21.367939256168906 26.38622665623767 30.6458928869843 21.59994120060912 40-41 21.475622653313117 26.141513842609427 30.762966719764563 21.6198967843129 42-43 21.761040527361743 26.364158259652122 29.959073372427795 21.91572784055834 44-45 21.72521751553414 26.549029227959664 29.738755783887733 21.986997472618466 46-47 22.019357491003895 26.84970734807561 29.097592184369304 22.03334297655119 48-49 22.315571420783826 26.82415349420374 28.610965429216456 22.249309655795976 50-51 21.93567508141883 27.04955613150829 28.326757016273984 22.688011770798898 52-53 22.3726307415606 27.346456864608648 27.976968341714585 22.30394405211617 54-55 21.953926530477016 26.99372286720361 28.115478461486916 22.936872140832463 56-57 22.145354281982677 27.407072669245963 27.955175775339008 22.492397273432356 58-59 22.344045681322136 26.889846274613895 28.167542042377068 22.5985660016869 60-61 22.352744796244682 27.09268673404248 28.445666876949787 22.108901592763054 62-63 22.08181541341935 27.074879036351128 28.457793367394892 22.38551218283463 64-65 21.84173585298887 26.873447857316577 28.38378155611997 22.901034733574583 66-67 22.00954670542763 27.274450387499964 27.96353560928646 22.75246729778594 68-69 21.918927342443833 27.02470166674123 28.247002380951486 22.809368609863448 70-71 22.04751363435052 27.065774227336675 28.320480362249263 22.566231776063546 72-73 22.17788592223193 26.971360635523194 28.197598352876774 22.6531550893681 74-75 21.82341297069478 27.45945600457183 27.81382941726055 22.90330160747284 76-77 21.99444481622703 27.25254506067617 27.94357819101621 22.809431932080585 78-79 22.142917699224338 27.270632151811025 27.87711155420051 22.709338594764123 80-81 21.813999162375154 27.66571851143388 27.79673837607551 22.723543950115456 82-83 22.050716192338058 27.37768337155532 27.721060942140678 22.85053949396594 84-85 21.806447295505933 27.149248883231213 27.611972002018113 23.43233181924474 86-87 21.98455469121695 27.08917980609432 27.718564014494522 23.207701488194203 88-89 21.991697344834638 27.07929178546938 27.695204882039437 23.23380598765655 90-91 21.946852483280846 27.28602238017729 27.85636369207321 22.91076144446865 92-93 22.022087034525747 27.086017547665442 27.84959702478456 23.042298393024243 94-95 21.934385674030043 26.970713798295726 27.7592737758879 23.33562675178633 96-97 21.989939992608576 27.02336494802881 27.802998602546996 23.18369645681562 98-99 21.734452270117817 26.98172416619234 27.51213764696948 23.771685916720365 100-101 21.601192387308572 27.003666339082226 27.86551167461316 23.529629598996042 102-103 22.079255511227917 26.600818725608494 27.847535899841553 23.472389863322036 104-105 21.95667588003176 26.574944058131422 27.9558961526431 23.512483909193715 106-107 21.74906213773384 26.84119792977608 27.995111104511043 23.41462882797904 108-109 21.943087462489274 26.916797059745722 27.472643770118193 23.66747170764681 110-111 21.523521149635474 27.094624467649957 27.61199276364861 23.769861619065953 112-113 21.720959635596078 26.72905108846912 27.704292502120847 23.845696773813955 114-115 21.327065176710626 26.946525611212003 27.538314887460974 24.1880943246164 116-117 21.4581831087264 26.981009855235488 27.63757441052428 23.923232625513833 118-119 21.443095486719756 27.01212182162867 27.438420450604305 24.106362241047268 120-121 21.239054335161885 27.195966077697065 27.53388075912751 24.031098828013533 122-123 21.44504367670031 27.068087530099987 27.318963037875697 24.16790575532401 124-125 21.343774914825488 27.003953285471805 27.309553212380482 24.342718587322228 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 362.0 1 306.5 2 392.0 3 611.0 4 518.0 5 452.5 6 893.0 7 2015.5 8 3375.0 9 3994.0 10 3348.0 11 2425.5 12 2185.0 13 2280.5 14 2552.0 15 2960.5 16 3390.5 17 3921.0 18 4555.0 19 5248.0 20 6018.0 21 7056.5 22 8236.5 23 9660.5 24 11462.5 25 13897.0 26 17474.5 27 21616.0 28 25597.0 29 29819.5 30 34785.0 31 40622.5 32 47351.5 33 53932.0 34 60613.0 35 65359.0 36 69800.0 37 75510.5 38 78822.5 39 79563.5 40 79306.5 41 82638.5 42 86752.0 43 88759.5 44 89954.5 45 91270.5 46 91278.5 47 91767.5 48 98469.0 49 100483.5 50 94842.5 51 88838.0 52 83416.5 53 81397.0 54 79862.5 55 73907.5 56 66528.0 57 60354.5 58 55775.5 59 50331.0 60 44326.0 61 37991.0 62 31421.5 63 25221.5 64 19836.5 65 15970.0 66 11901.5 67 9299.5 68 6757.5 69 4095.0 70 2989.0 71 2440.0 72 2013.0 73 1814.5 74 1499.0 75 1246.5 76 897.0 77 494.0 78 312.5 79 195.0 80 138.0 81 101.0 82 66.5 83 47.0 84 34.0 85 19.0 86 11.0 87 8.0 88 8.0 89 8.5 90 5.0 91 1.5 92 1.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10789854979837633 2 2.2557188111158816E-4 3 0.0 4 0.1870366847550252 5 0.0 6 1.1278594055579408E-4 7 0.040414962032492875 8 0.0 9 0.0 10-11 7.331086136126616E-4 12-13 6.391203298161664E-4 14-15 6.203226730568675E-4 16-17 0.004624223562787557 18-19 0.0015602055110218182 20-21 0.0032895899328773274 22-23 0.0019173609894484994 24-25 0.00513176029528863 26-27 0.0045490329357503615 28-29 0.0036091500977854106 30-31 8.646922109277546E-4 32-33 3.759531351859802E-5 34-35 0.0013910266001881271 36-37 0.0013346336299102299 38-39 5.451320460196714E-4 40-41 0.001466217227225323 42-43 9.022875244463527E-4 44-45 0.001447419570466024 46-47 2.0677422435228914E-4 48-49 3.759531351859802E-5 50-51 0.04221953708138559 52-53 7.519062703719604E-5 54-55 0.14124559288937277 56-57 1.8797656759299012E-4 58-59 1.879765675929901E-5 60-61 0.0 62-63 6.015250162975683E-4 64-65 0.06336690093559696 66-67 0.03283950635849538 68-69 0.001691789108336911 70-71 0.003007625081487842 72-73 0.07287851525580227 74-75 0.005300939206122322 76-77 0.0018045750488927053 78-79 0.001748182078614808 80-81 1.5038125407439208E-4 82-83 2.2557188111158816E-4 84-85 0.0 86-87 1.691789108336911E-4 88-89 0.0012782406596323328 90-91 2.8196485138948517E-4 92-93 0.0024436953787088716 94-95 0.0045490329357503615 96-97 0.002518886005746068 98-99 0.0 100-101 0.0012030500325951367 102-103 0.00152261019750322 104-105 7.519062703719604E-5 106-107 1.879765675929901E-5 108-109 0.0 110-111 7.519062703719604E-5 112-113 1.879765675929901E-5 114-115 2.443695378708872E-4 116-117 0.0 118-119 0.001259443002873034 120-121 0.0023685047516716754 122-123 7.895015838905586E-4 124-125 0.008252171317332265 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2659906.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.64595982814448 #Duplication Level Percentage of deduplicated Percentage of total 1 77.38255114205595 38.41731025397845 2 10.836201543341456 10.759472530208141 3 4.422405676866732 6.5866372373245134 4 2.2220720971562367 4.412676082826372 5 1.2827249872368784 3.1841056593459602 6 0.8338956742291622 2.483973068618667 7 0.5685040246801153 1.975674957998522 8 0.3977150779450595 1.5795957426166216 9 0.29591649167928913 1.3221952432555892 >10 1.5430187307545156 13.918028902593797 >50 0.1254169787554358 4.318932967907395 >100 0.08451265356499606 7.831968580705348 >500 0.00342856240479371 1.1399327126895455 >1k 0.0014025937110504758 1.0383070556406093 >5k 1.558437456722751E-4 0.608941555992505 >10k+ 7.792187283613756E-5 0.4222474482979602 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10915 0.41035284705549746 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9871 0.3711033397420811 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5870 0.2206844903541704 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2701 0.10154494181373326 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.639297027789704E-5 0.0 12-13 0.0 0.0 0.0 9.398828379649507E-5 0.0 14-15 0.0 0.0 0.0 1.1278594055579408E-4 0.0 16-17 0.0 0.0 0.0 2.2557188111158816E-4 0.0 18-19 0.0 0.0 0.0 2.443695378708872E-4 0.0 20-21 0.0 0.0 0.0 5.639297027789703E-4 0.0 22-23 0.0 0.0 0.0 0.0012030500325951369 0.0 24-25 0.0 0.0 0.0 0.0016541937948183131 0.0 26-27 0.0 0.0 0.0 0.0021429328705600877 0.0 28-29 0.0 0.0 0.0 0.0033271852463959252 0.0 30-31 0.0 0.0 0.0 0.006710763463069747 0.0 32-33 0.0 0.0 0.0 0.0133651339558616 0.0 34-35 0.0 0.0 0.0 0.022181234975972835 0.0 36-37 0.0 0.0 0.0 0.037350943980727136 0.0 38-39 0.0 0.0 0.0 0.06299094780041099 0.0 40-41 0.0 0.0 0.0 0.09558608462103549 0.0 42-43 0.0 0.0 0.0 0.1293654738174958 0.0 44-45 0.0 0.0 0.0 0.16761870532266931 0.0 46-47 0.0 0.0 0.0 0.21493240738582492 0.0 48-49 0.0 3.759531351859803E-5 0.0 0.2689944682255689 0.0 50-51 0.0 3.759531351859803E-5 0.0 0.3311395214718114 0.0 52-53 0.0 3.759531351859803E-5 0.0 0.3911980348177717 0.0 54-55 0.0 3.759531351859803E-5 0.0 0.4551852584264256 0.0 56-57 1.8797656759299014E-5 3.759531351859803E-5 0.0 0.5332143316342758 0.0 58-59 3.759531351859803E-5 3.759531351859803E-5 0.0 0.6233678934518738 0.0 60-61 3.759531351859803E-5 3.759531351859803E-5 0.0 0.723014271932918 0.0 62-63 3.759531351859803E-5 3.759531351859803E-5 0.0 0.8254051083008196 0.0 64-65 3.759531351859803E-5 3.759531351859803E-5 0.0 0.9266868829199227 3.759531351859803E-5 66-67 3.759531351859803E-5 3.759531351859803E-5 0.0 1.0469354932091584 3.759531351859803E-5 68-69 3.759531351859803E-5 3.759531351859803E-5 0.0 1.1912451041502972 3.759531351859803E-5 70-71 3.759531351859803E-5 3.759531351859803E-5 0.0 1.3612699095381566 3.759531351859803E-5 72-73 3.759531351859803E-5 3.759531351859803E-5 0.0 1.5307495828799964 3.759531351859803E-5 74-75 3.759531351859803E-5 3.759531351859803E-5 0.0 1.700774388267856 7.519062703719605E-5 76-77 3.759531351859803E-5 7.519062703719605E-5 0.0 1.8852357940468574 7.519062703719605E-5 78-79 3.759531351859803E-5 7.519062703719605E-5 0.0 2.1033638030817627 7.519062703719605E-5 80-81 3.759531351859803E-5 1.1278594055579408E-4 0.0 2.355064427088777 7.519062703719605E-5 82-83 3.759531351859803E-5 1.503812540743921E-4 0.0 2.622949833565547 7.519062703719605E-5 84-85 3.759531351859803E-5 1.503812540743921E-4 3.759531351859803E-5 2.885534300836195 7.519062703719605E-5 86-87 3.759531351859803E-5 1.503812540743921E-4 3.759531351859803E-5 3.1563521417674156 7.519062703719605E-5 88-89 3.759531351859803E-5 1.503812540743921E-4 3.759531351859803E-5 3.4686376135096504 7.519062703719605E-5 90-91 3.759531351859803E-5 1.503812540743921E-4 3.759531351859803E-5 3.8324474624291236 7.519062703719605E-5 92-93 3.759531351859803E-5 1.503812540743921E-4 3.759531351859803E-5 4.220224323716703 7.519062703719605E-5 94-95 3.759531351859803E-5 1.503812540743921E-4 3.759531351859803E-5 4.60698611153928 7.519062703719605E-5 96-97 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 4.993277957942874 7.519062703719605E-5 98-99 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 5.415285352189137 7.519062703719605E-5 100-101 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 5.897802403543584 7.519062703719605E-5 102-103 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 6.432332571151011 7.519062703719605E-5 104-105 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 6.966862738758437 7.519062703719605E-5 106-107 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 7.4919376850159365 7.519062703719605E-5 108-109 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 8.035208763016438 7.519062703719605E-5 110-111 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 8.644516009212357 7.519062703719605E-5 112-113 3.759531351859803E-5 1.8797656759299012E-4 3.759531351859803E-5 9.301738482487727 7.519062703719605E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12665 0.0 42.254078 1 GTATCAA 18730 0.0 39.24229 1 GTACATG 14675 0.0 31.71012 1 TATCAAC 23570 0.0 30.567293 2 ATCAACG 23210 0.0 30.142775 3 TCAACGC 23260 0.0 30.129221 4 TACATGG 15180 0.0 30.0317 2 CAACGCA 23400 0.0 29.898272 5 AACGCAG 23855 0.0 29.153435 6 ACATGGG 15535 0.0 27.695946 3 GAGTACT 14275 0.0 27.163599 12-13 CGCAGAG 27345 0.0 25.46704 8 AGTACTT 14920 0.0 25.43125 12-13 ACGCAGA 27180 0.0 25.236813 7 GTACTTT 15590 0.0 25.21571 14-15 AGAGTAC 22190 0.0 24.777954 10-11 GCAGAGT 27750 0.0 23.9381 9 CATGGGG 5190 0.0 23.768034 4 CATGGGA 10385 0.0 23.527056 4 AGCGTAC 300 5.5606506E-9 21.813538 5 >>END_MODULE