FastQCFastQC Report
Thu 26 May 2016
SRR1364493_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364493_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3271512
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65300.048.581981
GGTATCA38200.046.5874561
ATCAACG75500.039.7960173
CAACGCA77400.039.073025
TCAACGC78150.038.3933264
AACGCAG78400.038.3316
TATCAAC86000.036.597582
ACGCAGA94850.030.931747
GTACATG118550.029.8728471
CGCAGAG95150.029.6476928
TACATGG115400.028.8720322
ACATGGG116000.026.9788193
GCAGAGT109650.024.0445389
CATGGGG64250.022.2378254
AGAGTAC101350.021.63459410-11
TATACCG2107.121099E-519.844175
GTATCGA2751.3152548E-619.4791951
CAGAGTA112200.019.33034910-11
GAGTACA91750.018.8127861
GAGTACT47600.018.7460512-13
Produced by FastQC (version 0.11.5)