##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364493_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3271512 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.25572212481568 31.0 27.0 31.0 10.0 34.0 2 27.52594488420033 31.0 27.0 31.0 16.0 34.0 3 27.52650731527196 31.0 27.0 31.0 15.0 34.0 4 30.43933416719853 35.0 30.0 37.0 10.0 37.0 5 31.057477093160593 35.0 31.0 37.0 17.0 37.0 6 31.327750287940255 35.0 32.0 37.0 17.0 37.0 7 31.185879801143937 35.0 32.0 37.0 17.0 37.0 8 30.851709545922496 35.0 31.0 37.0 11.0 37.0 9 31.635482920435567 37.0 30.0 39.0 10.0 39.0 10-11 31.752679647820337 37.0 30.5 39.0 10.5 39.0 12-13 31.60854690430602 37.0 30.0 39.0 10.0 39.0 14-15 32.36504817955734 37.0 30.5 40.0 10.0 41.0 16-17 32.63091026412252 38.0 31.0 40.0 10.0 41.0 18-19 32.529574857130285 38.0 31.0 40.0 10.0 41.0 20-21 32.28799160755027 37.5 30.5 40.0 10.0 41.0 22-23 32.354796803435235 38.0 30.5 40.0 10.0 41.0 24-25 32.39488438373449 38.0 31.0 40.0 9.5 41.0 26-27 32.51871153154872 38.0 31.0 40.0 9.0 41.0 28-29 32.48173061874754 38.0 31.0 40.0 8.0 41.0 30-31 32.128929987113 37.0 30.0 40.0 8.0 41.0 32-33 32.186761809218496 37.0 30.0 40.0 8.0 41.0 34-35 31.90084355490672 37.0 30.0 40.0 2.0 41.0 36-37 31.686980973934986 37.0 30.0 40.0 2.0 41.0 38-39 31.576192292738035 37.0 30.0 40.0 2.0 41.0 40-41 31.652976360777522 37.0 30.0 40.0 2.0 41.0 42-43 31.564429535945457 37.0 30.0 40.0 2.0 41.0 44-45 31.228802309146353 37.0 29.5 40.0 2.0 41.0 46-47 31.02886784459296 36.5 29.0 40.0 2.0 41.0 48-49 30.8389576440496 36.0 28.5 40.0 2.0 41.0 50-51 30.85767483047594 36.0 29.0 40.0 2.0 41.0 52-53 30.717535958908297 36.0 28.5 40.0 2.0 41.0 54-55 30.62526516791013 36.0 28.5 40.0 2.0 41.0 56-57 30.24220666162924 36.0 28.0 40.0 2.0 41.0 58-59 29.989192306187476 35.0 28.0 39.5 2.0 41.0 60-61 29.63004460934271 35.0 26.5 39.0 2.0 41.0 62-63 29.56225775726942 35.0 27.0 39.0 2.0 40.0 64-65 29.365868748150703 34.5 27.0 39.0 2.0 40.0 66-67 29.13067551028393 34.0 26.0 38.5 2.0 40.0 68-69 28.939356022536366 34.0 26.5 38.0 2.0 40.0 70-71 28.78868318380003 34.0 26.5 37.5 2.0 40.0 72-73 28.77841041084367 34.0 27.0 37.0 2.0 40.0 74-75 28.48318376946195 34.0 26.5 37.0 2.0 39.0 76-77 28.1663947740372 34.0 26.0 36.0 2.0 39.0 78-79 27.859341491029227 34.0 26.0 36.0 2.0 39.0 80-81 27.830770756763233 34.0 26.0 35.5 2.0 37.5 82-83 27.62639354524758 34.0 26.0 35.0 2.0 37.0 84-85 27.406616420786477 34.0 26.0 35.0 2.0 36.5 86-87 27.18734915231856 33.0 26.0 35.0 2.0 36.0 88-89 26.963763391361546 33.0 26.0 35.0 2.0 36.0 90-91 26.783239676333146 33.0 25.5 35.0 2.0 36.0 92-93 26.663914116775363 33.0 25.0 35.0 2.0 35.0 94-95 26.609068375723517 33.0 25.0 35.0 2.0 35.0 96-97 26.484218612066833 33.0 25.0 35.0 2.0 35.0 98-99 26.398086419979506 33.0 25.0 35.0 2.0 35.0 100-101 26.339426998892257 33.0 25.0 35.0 2.0 35.0 102-103 25.15705887675179 31.5 21.5 34.0 2.0 35.0 104-105 25.999414796583352 33.0 24.0 35.0 2.0 35.0 106-107 26.073861260481394 33.0 24.0 35.0 2.0 35.0 108-109 26.039868721251825 33.0 24.0 35.0 2.0 35.0 110-111 25.95051156773993 33.0 24.0 35.0 2.0 35.0 112-113 25.937109354940468 33.0 24.0 35.0 2.0 35.0 114-115 25.89144316144951 33.0 23.5 35.0 2.0 35.0 116-117 25.700645297953976 33.0 22.5 35.0 2.0 35.0 118-119 25.586302449754122 33.0 21.5 35.0 2.0 35.0 120-121 25.459825762522037 33.0 19.5 35.0 2.0 35.0 122-123 25.193077543349986 33.0 14.0 35.0 2.0 35.0 124-125 24.166665902494017 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.7514544268950054 1101 2 1.3418267541466484 1101 3 1.6501941534411344 1101 4 1.991916965239632 1101 5 1.394486275607079 1101 6 1.3328328918814378 1101 7 1.160453638764185 1101 8 1.6413040503140124 1101 9 2.119340309608372 1101 10-11 2.1265048655495526 1101 12-13 1.9434949556312873 1101 14-15 2.230383431984329 1101 16-17 1.8344822743526024 1101 18-19 1.7722048540543867 1101 20-21 2.225912978472735 1101 22-23 2.0102286698140475 1101 24-25 1.876198638370525 1101 26-27 1.5859849434464834 1101 28-29 1.5577475552578903 1101 30-31 1.7516121362205368 1101 32-33 1.9374993994796625 1101 34-35 2.2331354694131846 1101 36-37 2.240898342283149 1101 38-39 2.2885255804554347 1101 40-41 2.0277885213780564 1101 42-43 2.048118703890623 1101 44-45 2.1165162088757015 1101 46-47 1.992940405615773 1101 48-49 2.034134541642864 1101 50-51 1.9759303314378869 1101 52-53 1.976893009436811 1101 54-55 1.9518293390571877 1101 56-57 2.2929809090040507 1101 58-59 2.2637843385924086 1101 60-61 2.54345289669288 1101 62-63 2.0954400227764474 1101 64-65 1.9963769639984896 1101 66-67 2.1521420544257914 1101 68-69 1.9372764884079174 1101 70-71 1.97885738868019 1101 72-73 1.7764150155877338 1101 74-75 1.7772445471566485 1101 76-77 1.9966188597020889 1101 78-79 1.935850601563338 1101 80-81 1.8010153762207821 1101 82-83 1.8352982793707753 1101 84-85 1.6864473135990323 1101 86-87 1.6149287820667553 1101 88-89 1.7655364131792126 1101 90-91 1.793245827767116 1101 92-93 1.7805134838068817 1101 94-95 1.7731576142186327 1101 96-97 1.8308580295207442 1101 98-99 1.6868317199264133 1101 100-101 1.7220225407926044 1101 102-103 1.7649241877724329 1101 104-105 1.7096189750771558 1101 106-107 1.8983180464200977 1101 108-109 1.9414466495275207 1101 110-111 2.0576832813447545 1101 112-113 1.9789193037687305 1101 114-115 1.9608725447927107 1101 116-117 2.12270471188047 1101 118-119 2.1148960888869155 1101 120-121 2.0015013895266165 1101 122-123 2.0803368476931396 1101 124-125 2.1089809960564416 1102 1 0.8434040701120686 1102 2 0.6206318769086714 1102 3 0.7778496213228578 1102 4 1.2400173619717378 1102 5 0.9994903095752008 1102 6 0.9202550274451582 1102 7 0.8980114374958639 1102 8 1.1257722967758 1102 9 1.5208878941609392 1102 10-11 1.2578436398584678 1102 12-13 1.4457587257292062 1102 14-15 1.815626456760107 1102 16-17 1.2833246042549078 1102 18-19 1.401137322121734 1102 20-21 1.5131309228750496 1102 22-23 1.3988212158636344 1102 24-25 1.2031721098558705 1102 26-27 1.2393052806075886 1102 28-29 1.052635405346841 1102 30-31 0.9464085874480546 1102 32-33 0.8714481505115899 1102 34-35 1.2617760410312755 1102 36-37 1.4974517018275186 1102 38-39 1.5770193046996681 1102 40-41 1.181949040766746 1102 42-43 1.183921625608221 1102 44-45 1.576108530121715 1102 46-47 1.3271369287231671 1102 48-49 1.4217137468300791 1102 50-51 1.3292676850714926 1102 52-53 1.4795566454856761 1102 54-55 1.264908400276397 1102 56-57 1.392741532893215 1102 58-59 1.4170806431183465 1102 60-61 1.8279960266980808 1102 62-63 1.55158088662618 1102 64-65 1.677231948506961 1102 66-67 1.6296069599445069 1102 68-69 1.387859418930205 1102 70-71 1.7284595078216611 1102 72-73 1.575806636831583 1102 74-75 1.6137911105256144 1102 76-77 1.500687013717144 1102 78-79 1.456377368289079 1102 80-81 1.089243032996869 1102 82-83 1.079109867676408 1102 84-85 1.134353265648052 1102 86-87 1.2548946052839298 1102 88-89 0.9999284451093899 1102 90-91 0.9396757139126315 1102 92-93 1.0033115184222048 1102 94-95 1.2590741971209063 1102 96-97 1.2858692293470462 1102 98-99 1.3520555335635933 1102 100-101 1.2358820980428007 1102 102-103 0.9941659462545047 1102 104-105 1.0236497416664747 1102 106-107 1.2607892007493824 1102 108-109 1.3898509085855935 1102 110-111 1.1959095290306543 1102 112-113 1.155545846185536 1102 114-115 1.1355710417978884 1102 116-117 1.3467824802512958 1102 118-119 1.4795493804565112 1102 120-121 1.4247658850619196 1102 122-123 1.5130743994715061 1102 124-125 1.476531497458243 1103 1 1.3373769254186136 1103 2 0.9891522123804073 1103 3 1.2628107659106718 1103 4 1.8066961861556905 1103 5 1.2398738080764389 1103 6 1.1481097212164286 1103 7 1.1781790728137018 1103 8 1.4602942526839726 1103 9 2.1586292350802516 1103 10-11 1.707331832539058 1103 12-13 1.8751008610528217 1103 14-15 2.0939034414372557 1103 16-17 1.9009455805296653 1103 18-19 1.608188753969742 1103 20-21 2.031335417539225 1103 22-23 1.9323510706975213 1103 24-25 1.8852770442473954 1103 26-27 1.6665499667049986 1103 28-29 1.5310382320806042 1103 30-31 1.8337047621329035 1103 32-33 1.7062081965743445 1103 34-35 1.6479848785800115 1103 36-37 1.9686047763963863 1103 38-39 2.0328533524993517 1103 40-41 2.105244965902557 1103 42-43 2.0562507506254235 1103 44-45 1.976508836945591 1103 46-47 2.08210745653766 1103 48-49 2.2005767179640756 1103 50-51 1.9719771542953097 1103 52-53 2.0236377823532656 1103 54-55 1.970947091999566 1103 56-57 2.2726432502698586 1103 58-59 2.390071415876129 1103 60-61 2.3948946753789357 1103 62-63 2.0844005354724224 1103 64-65 1.964810282714236 1103 66-67 1.997322741348686 1103 68-69 1.9607215028657805 1103 70-71 2.0597387953101816 1103 72-73 1.9744416225229955 1103 74-75 1.9920296573379659 1103 76-77 2.056685194529461 1103 78-79 2.2752618155271804 1103 80-81 1.7509081088047367 1103 82-83 1.8366498626109937 1103 84-85 1.7764782175027811 1103 86-87 1.7667809779410852 1103 88-89 1.8359495724194232 1103 90-91 1.805667912599631 1103 92-93 1.815679892240663 1103 94-95 1.5857933056497302 1103 96-97 1.7128041011613035 1103 98-99 1.5717091592362493 1103 100-101 1.4398214307256865 1103 102-103 1.4863182264060093 1103 104-105 1.694563247199092 1103 106-107 1.5091081399609472 1103 108-109 1.7367933648102074 1103 110-111 1.83883774542036 1103 112-113 1.6999176828723392 1103 114-115 1.6127320160106997 1103 116-117 1.812966708294983 1103 118-119 1.882404924666357 1103 120-121 1.8059769677031987 1103 122-123 1.8036399114156154 1103 124-125 1.7656343856390535 1104 1 1.0650766873742405 1104 2 0.9027470164369085 1104 3 0.9550316583240388 1104 4 1.1360753462920705 1104 5 0.7831739396751622 1104 6 0.6699619277947662 1104 7 0.7686981935178245 1104 8 1.18348017084746 1104 9 1.7373968936524768 1104 10-11 1.4854463835091956 1104 12-13 0.9596073608432931 1104 14-15 1.3314811785424396 1104 16-17 0.9370929137671098 1104 18-19 0.3058389975108966 1104 20-21 -4.17428790397873 1104 22-23 -0.8183644771223726 1104 24-25 0.4601114801365753 1104 26-27 0.5707089065063755 1104 28-29 0.18058211747628405 1104 30-31 0.8697294148945005 1104 32-33 0.7994178119814919 1104 34-35 1.0326186145703815 1104 36-37 1.1353787092787933 1104 38-39 1.1008867966242306 1104 40-41 1.2110459232652175 1104 42-43 1.1098374391362995 1104 44-45 1.27950340597409 1104 46-47 1.2267865640495081 1104 48-49 1.2421805746368833 1104 50-51 1.1524194434301016 1104 52-53 1.1038090214877734 1104 54-55 1.0423824342472408 1104 56-57 1.2708142610572608 1104 58-59 1.3214946190823618 1104 60-61 1.4282284595066663 1104 62-63 1.1451556339570779 1104 64-65 1.1632683726705295 1104 66-67 1.1367243759748824 1104 68-69 1.0899704478199226 1104 70-71 1.3506917630372115 1104 72-73 1.1689048223688339 1104 74-75 1.1374137491235246 1104 76-77 1.1559990439056307 1104 78-79 1.1306501713707355 1104 80-81 0.9042320922051985 1104 82-83 0.9103614465147167 1104 84-85 0.8639440682689781 1104 86-87 0.7165125205279423 1104 88-89 0.9382386648319425 1104 90-91 0.9504715079197155 1104 92-93 0.692840184175946 1104 94-95 0.8750936548762027 1104 96-97 0.9514968377043438 1104 98-99 0.9120815432472682 1104 100-101 0.3943872135112585 1104 102-103 0.861122504773089 1104 104-105 0.8969465203254074 1104 106-107 1.0166825765032605 1104 108-109 1.0636656302851506 1104 110-111 1.1156949227859805 1104 112-113 1.0891656630849162 1104 114-115 1.017729994291301 1104 116-117 1.0822278546123734 1104 118-119 1.0227615786534443 1104 120-121 1.0630322012766023 1104 122-123 0.9707153883581654 1104 124-125 1.1283197836647467 1105 1 1.080571290144789 1105 2 0.8964458503297195 1105 3 0.8407012023066578 1105 4 1.4402997327009253 1105 5 1.0320724514533595 1105 6 0.951831946906502 1105 7 0.9225334335002522 1105 8 1.206932256298316 1105 9 1.6611729033521137 1105 10-11 1.3286583005435695 1105 12-13 1.4539684700274833 1105 14-15 1.5916699131768937 1105 16-17 1.3298535159525429 1105 18-19 1.1007470430372308 1105 20-21 1.278509838403977 1105 22-23 1.1356386816920008 1105 24-25 1.1236630652239938 1105 26-27 1.050859749443326 1105 28-29 0.7224314439410549 1105 30-31 1.106540271584656 1105 32-33 1.0762664145931922 1105 34-35 0.9239531256729876 1105 36-37 1.1105709701835913 1105 38-39 1.0371176032076832 1105 40-41 1.0694627761732463 1105 42-43 1.2854647407628619 1105 44-45 1.4384032297359752 1105 46-47 1.7372112421108348 1105 48-49 1.3474259834479838 1105 50-51 1.422188549381083 1105 52-53 1.3597118170686464 1105 54-55 1.3812254707304419 1105 56-57 1.7844918166858719 1105 58-59 1.483318381972225 1105 60-61 1.8112406081740247 1105 62-63 1.6507730353848835 1105 64-65 1.392495737433162 1105 66-67 1.5300652544711149 1105 68-69 1.1302576465789151 1105 70-71 1.2154314599438436 1105 72-73 1.2688281517511406 1105 74-75 1.5212075585712164 1105 76-77 1.488272171262551 1105 78-79 1.3270287445155873 1105 80-81 1.2694493939021285 1105 82-83 1.3876021110721553 1105 84-85 1.3661785876154582 1105 86-87 1.333486359774252 1105 88-89 1.413926187012514 1105 90-91 1.4204045238635814 1105 92-93 1.4046209934235705 1105 94-95 1.2187938585604172 1105 96-97 1.4033461566289773 1105 98-99 1.2956389065923517 1105 100-101 1.3949462517719198 1105 102-103 1.4532791169195427 1105 104-105 1.4738696069153256 1105 106-107 1.4795115275094695 1105 108-109 1.6116257344194551 1105 110-111 1.598381576342863 1105 112-113 1.5812012057786724 1105 114-115 1.5070273365441125 1105 116-117 1.6838795717024446 1105 118-119 1.7595248068169553 1105 120-121 1.7266000604912008 1105 122-123 1.9082248135283493 1105 124-125 1.4447042104686254 1106 1 -0.5151155614855654 1106 2 -0.4378425945212392 1106 3 -0.3653615180510741 1106 4 -0.8093946003431576 1106 5 -1.175449418196493 1106 6 -0.8035756611518501 1106 7 -0.6953713406892632 1106 8 -0.25967265157163055 1106 9 -0.21196084743941768 1106 10-11 -2.621080592644983 1106 12-13 -0.8651022946089633 1106 14-15 -0.5350761409050477 1106 16-17 -0.6994224012437655 1106 18-19 -3.468606522165203 1106 20-21 -6.012132922136065 1106 22-23 -6.926922393067418 1106 24-25 -6.714718658862715 1106 26-27 -5.613433698651924 1106 28-29 -2.2345866810931447 1106 30-31 -4.126832051875084 1106 32-33 -4.766682822021174 1106 34-35 -3.9402516907314045 1106 36-37 -6.5911065824481945 1106 38-39 -7.023422239033774 1106 40-41 -3.114620848364538 1106 42-43 -1.6396383534310317 1106 44-45 -0.9184971431620745 1106 46-47 -0.6749592473471786 1106 48-49 -0.3992537971910224 1106 50-51 -0.5413112200006296 1106 52-53 -0.7541350523857631 1106 54-55 -0.6472046831974971 1106 56-57 -0.5564845586199922 1106 58-59 -0.4352129703890739 1106 60-61 -0.1727161763034104 1106 62-63 -0.4618656287488925 1106 64-65 -0.666054826604455 1106 66-67 -0.9305095713925304 1106 68-69 -0.8499388871108984 1106 70-71 -0.6608253120254801 1106 72-73 -0.5963669552322948 1106 74-75 -0.6652559294718117 1106 76-77 -0.7053790830664255 1106 78-79 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-2.4700610587026333 2216 78-79 -2.692255823860375 2216 80-81 -2.7725633493231214 2216 82-83 -3.0253469201615637 2216 84-85 -2.857339733355044 2216 86-87 -3.1585085007614424 2216 88-89 -3.3547798298406306 2216 90-91 -3.356939969094981 2216 92-93 -3.194133420396337 2216 94-95 -6.839968555954126 2216 96-97 -9.189443088121987 2216 98-99 -5.334249683218026 2216 100-101 -4.2842152464873635 2216 102-103 -5.459413974780407 2216 104-105 -8.466355487563163 2216 106-107 -4.483787508272453 2216 108-109 -7.334968595217433 2216 110-111 -4.320882628507999 2216 112-113 -3.6349127514021013 2216 114-115 -4.1540375000928655 2216 116-117 -3.582049459862052 2216 118-119 -3.858020799209818 2216 120-121 -4.6148963588361624 2216 122-123 -7.903944615135227 2216 124-125 -6.359995629104009 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 220989.0 3 34933.0 4 20427.0 5 16571.0 6 16256.0 7 18536.0 8 20656.0 9 22011.0 10 23547.0 11 24388.0 12 25281.0 13 25458.0 14 25908.0 15 23124.0 16 19442.0 17 17781.0 18 16278.0 19 15604.0 20 15905.0 21 17196.0 22 21449.0 23 28973.0 24 38609.0 25 50332.0 26 61881.0 27 72645.0 28 84161.0 29 96952.0 30 111833.0 31 128188.0 32 154625.0 33 193405.0 34 243567.0 35 313974.0 36 423434.0 37 477322.0 38 149402.0 39 469.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.51452930319613 19.81290032639498 14.343757638649032 23.32881273175986 2 14.808648685009118 20.56822160153246 41.074588257956215 23.5485414555022 3 18.356165221326613 25.94731949557836 29.32550118032707 26.37101410276796 4 11.152717271310015 17.17152658557186 39.46708370004177 32.20867244307635 5 13.644934636325493 38.136924268167824 34.519384026725284 13.6987570687814 6 30.71497763813051 37.45985302393427 18.41804331272962 13.40712602520559 7 27.69026296017113 32.310941400224856 22.159375395467325 17.83942024413669 8 24.939369244510527 35.83658215551429 20.73648256640344 18.48756603357174 9 25.367694035521897 16.84517692170839 21.058106570591693 36.729022472178016 10-11 23.852591464510017 27.15775733129524 28.552564449304906 20.437086754889837 12-13 24.788435449427176 24.352528717572177 28.781215782391445 22.077820050609198 14-15 22.258111060976788 25.864083403949362 26.17132426758033 25.706481267493515 16-17 22.17836693332958 29.050293676721267 26.433014451710463 22.338324938238692 18-19 22.02115058406924 28.15860477010896 28.181518437859555 21.638726207962247 20-21 22.953912855452767 27.344179077663362 28.107822068826422 21.594085998057455 22-23 22.6059463620461 27.26565418603613 27.746280848732606 22.382118603185162 24-25 22.26014069475487 27.398638909244212 27.921131207595838 22.42008918840508 26-27 22.291090096491615 27.603593105597575 27.641696984444046 22.463619813466764 28-29 22.29370244169202 27.68471644526666 27.68331031042261 22.338270802618712 30-31 22.52539435549209 27.395473957918604 27.760633640389244 22.318498046200066 32-33 22.371829849281717 27.38871133650329 27.711270780889148 22.528188033325844 34-35 22.49069836407383 27.45859740196213 27.737172608401895 22.31353162556215 36-37 22.666549490858376 27.215207382075924 27.711869782014787 22.406373345050916 38-39 22.349025427401017 27.44456293449501 27.76737487101661 22.439036767087366 40-41 22.580897661259474 27.349403963466372 27.68262304385142 22.387075331422732 42-43 22.466498221543553 27.37431960857144 27.50106615674568 22.65811601313933 44-45 22.48012713249787 27.362632537419067 27.66390975269473 22.493330577388328 46-47 22.55797901753959 27.55198922540479 27.34444804806751 22.54558370898811 48-49 22.555229348375004 27.502546413195127 27.342455586029637 22.599768652400233 50-51 22.562841759745048 27.55768694329702 27.245998234599554 22.633473062358377 52-53 22.68088400147837 27.513373651209307 27.19246410536166 22.613278241950667 54-55 22.666187337800444 27.415501856253005 27.23148909412194 22.686821711824614 56-57 22.442244224422442 27.594736040083884 27.201701321431177 22.761318414062494 58-59 22.566760988446603 27.396691359896696 27.274651857250465 22.761895794406236 60-61 22.737947316863178 27.298558362001806 27.324806373145517 22.638687947989496 62-63 22.53728822496815 27.468145382236308 27.2922982630034 22.702268129792138 64-65 22.47661340169314 27.295129975135403 27.314403552329676 22.913853070841782 66-67 22.361138177457924 27.481408285588298 27.153188390180304 23.004265146773477 68-69 22.499386712173514 27.364650624636518 27.374646643042027 22.761316020147937 70-71 22.35987564880876 27.10470810483649 27.53876040080944 22.996655845545305 72-73 22.52691854064684 27.294103406383112 27.21239752950528 22.966580523464764 74-75 22.499922053579454 27.429024434827316 27.202949248015727 22.8681042635775 76-77 22.57177662429767 27.230042858103605 27.317211105201057 22.88096941239767 78-79 22.482597409108934 27.338767888310358 27.300078479425217 22.878556223155496 80-81 22.46609004678853 27.420434817097096 27.20884484007946 22.90463029603492 82-83 22.611210882274275 27.502563906614192 27.132612433609722 22.753612777501814 84-85 22.592099606152573 27.43946901743251 26.99119609090113 22.977235285513782 86-87 22.462059526365945 27.28012331083792 27.32952100892353 22.92829615387261 88-89 22.595283969660258 27.21288408802107 27.271208579447098 22.92062336287157 90-91 22.510214721211817 27.403820068066043 27.323777431644704 22.762187779077433 92-93 22.493442095032652 27.379572288417293 27.13415999438778 22.99282562216227 94-95 22.495835881935598 27.24543704669651 27.259880399865377 22.998846671502516 96-97 22.511186143915037 27.16649579484132 27.260643419539544 23.061674641704098 98-99 22.288586154288677 27.163780220263927 27.268229668962192 23.279403956485204 100-101 22.343431404000725 27.0939788322153 27.298002542310577 23.264587221473402 102-103 22.33034815286227 26.988907820096873 27.369590162781897 23.31115386425896 104-105 22.363549551818707 26.993049552430058 27.37399753747886 23.269403358272374 106-107 22.18116210044964 27.0319028943998 27.45724425261884 23.32969075253172 108-109 22.194243031974224 26.978226056166033 27.25719773839294 23.5703331734668 110-111 22.102035707178757 27.095734206587125 27.388831167163847 23.413398919070268 112-113 22.250064651496338 27.058683995101767 27.386511405257586 23.304739948144302 114-115 22.228882563093443 27.12237357430793 27.265017289799975 23.383726572798654 116-117 21.987749430053206 27.096147838298346 27.491625801884112 23.42447692976434 118-119 22.12330124880384 27.09069986783988 27.288703145019554 23.49729573833672 120-121 22.10169111228733 27.111674120615646 27.374840984884187 23.411793782212836 122-123 22.075084203748236 27.153353148617228 27.36788031841603 23.403682329218505 124-125 22.213725201455087 27.073847775694553 27.118438020248192 23.593989002602168 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 160.0 1 175.0 2 229.5 3 274.0 4 254.0 5 225.0 6 238.0 7 310.5 8 418.0 9 498.5 10 538.5 11 592.0 12 679.0 13 761.5 14 922.0 15 1069.0 16 1202.5 17 1411.5 18 1635.0 19 2011.0 20 2443.5 21 3080.0 22 4141.0 23 5536.0 24 7443.5 25 10285.0 26 14768.5 27 20491.5 28 26517.5 29 33269.5 30 41176.5 31 49395.5 32 56806.0 33 64220.0 34 71992.0 35 79742.5 36 87366.5 37 93821.5 38 98021.0 39 101296.0 40 105132.5 41 108957.5 42 111866.5 43 114852.5 44 119155.0 45 122671.0 46 123680.5 47 123177.5 48 123735.0 49 123129.0 50 119004.0 51 114937.5 52 112717.0 53 109910.0 54 104670.0 55 96922.5 56 88679.0 57 81007.0 58 74377.5 59 67064.5 60 56860.5 61 45614.5 62 35262.0 63 26489.0 64 19276.0 65 13938.5 66 10306.0 67 7939.0 68 6166.5 69 4765.5 70 3678.5 71 2743.5 72 2039.0 73 1601.0 74 1195.5 75 834.5 76 579.0 77 370.0 78 235.5 79 156.5 80 106.0 81 78.0 82 56.0 83 42.0 84 32.0 85 28.5 86 23.5 87 20.0 88 16.0 89 11.5 90 10.0 91 7.0 92 6.0 93 7.5 94 5.5 95 4.5 96 4.0 97 3.0 98 3.5 99 3.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08476203052288973 2 0.029252529105807955 3 0.03765842827414358 4 0.04371067567534522 5 0.04585035910001247 6 0.0029038560763341233 7 0.0020174158004005486 8 0.0021396834246672487 9 0.005440909279868147 10-11 0.034586454214442744 12-13 0.0051963740313347466 14-15 0.009170071820002495 16-17 0.0199754731145721 18-19 0.016460278916904478 20-21 0.12677624291153447 22-23 0.014366445851337239 24-25 0.014030209884603816 26-27 0.0058535625117682585 28-29 0.004065398506867772 30-31 0.002032699253433886 32-33 0.005410342373801472 34-35 0.01778993933080484 36-37 0.0022772345019672863 38-39 0.007870978312168807 40-41 0.030291803912074908 42-43 0.012746399829803468 44-45 0.10470693673139515 46-47 0.0034540603855342726 48-49 0.007458325080268695 50-51 0.009154788366969157 52-53 0.010423314968736169 54-55 0.008298914997102256 56-57 0.005272791296501434 58-59 0.013449438669336993 60-61 0.024086721980539884 62-63 0.006250932290635033 64-65 0.005929979776934946 66-67 0.007015104942301908 68-69 0.006403766820968409 70-71 0.004034831600801097 72-73 6.571884804335122E-4 74-75 0.0010392748062669494 76-77 0.007702860328802095 78-79 0.018065041485404915 80-81 0.0027663049990340857 82-83 0.004187666131134473 84-85 0.002063266159500561 86-87 0.0031942416839675354 88-89 0.0048601380646013225 90-91 0.003988981241701085 92-93 0.002399502126233986 94-95 0.0033929265734009228 96-97 0.001650612927600449 98-99 0.0022313841428672735 100-101 0.002399502126233986 102-103 5.502043092001496E-4 104-105 0.002078549612533899 106-107 6.724719334668495E-4 108-109 0.0010087079002002743 110-111 0.002109116518600574 112-113 0.0038514301644010473 114-115 0.0022772345019672863 116-117 0.004707303534267947 118-119 0.0031025409657675107 120-121 0.006220365384568358 122-123 0.00401954814776776 124-125 0.05314056619691445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3271512.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.47937884458873 #Duplication Level Percentage of deduplicated Percentage of total 1 80.73524431618235 51.250231600573684 2 10.811355578712742 13.725962732093281 3 3.6652787708468075 6.980088589968392 4 1.6147686427724632 4.100180416836622 5 0.8695913771073087 2.760056023369124 6 0.532375802548499 2.0276931154600883 7 0.3428494278361868 1.5234708101384655 8 0.24622212743134383 1.2504022165707904 9 0.18358013237462734 1.0488197494213847 >10 0.9201986344009427 10.437430179553122 >50 0.056153644355246085 2.405922114101188 >100 0.02203466516254682 2.264669211353544 >500 1.9821729658715917E-4 0.09742163885993164 >1k 1.4866297242763682E-4 0.12765160170035478 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 3.056690606667498E-5 16-17 0.0 0.0 0.0 0.0 3.056690606667498E-5 18-19 0.0 3.056690606667498E-5 0.0 3.056690606667498E-5 3.056690606667498E-5 20-21 0.0 3.056690606667498E-5 0.0 7.641726516668745E-5 3.056690606667498E-5 22-23 0.0 3.056690606667498E-5 0.0 1.8340143640004986E-4 3.056690606667498E-5 24-25 0.0 9.170071820002494E-5 0.0 3.5151941976676226E-4 4.585035910001247E-5 26-27 0.0 9.170071820002494E-5 0.0 4.7378704403346224E-4 6.113381213334996E-5 28-29 0.0 9.170071820002494E-5 0.0 9.017237289669119E-4 6.113381213334996E-5 30-31 0.0 9.170071820002494E-5 0.0 0.002185533783767261 6.113381213334996E-5 32-33 0.0 9.170071820002494E-5 0.0 0.005288074749534771 6.113381213334996E-5 34-35 0.0 9.170071820002494E-5 0.0 0.009781409941335993 6.113381213334996E-5 36-37 0.0 9.170071820002494E-5 0.0 0.017866356595971526 6.113381213334996E-5 38-39 0.0 1.2226762426669992E-4 0.0 0.032217518994275426 6.113381213334996E-5 40-41 0.0 1.2226762426669992E-4 0.0 0.05049652882214707 6.113381213334996E-5 42-43 0.0 1.2226762426669992E-4 0.0 0.06900479044551877 6.113381213334996E-5 44-45 0.0 1.2226762426669992E-4 0.0 0.09141033259239154 7.641726516668745E-5 46-47 0.0 1.2226762426669992E-4 0.0 0.12003624012383265 9.170071820002494E-5 48-49 0.0 1.2226762426669992E-4 0.0 0.15268169580304153 9.170071820002494E-5 50-51 0.0 1.528345303333749E-4 0.0 0.1909056118394186 1.528345303333749E-4 52-53 0.0 1.528345303333749E-4 0.0 0.230505038648796 2.1396834246672485E-4 54-55 0.0 1.681179833667124E-4 0.0 0.27453666683784134 2.1396834246672485E-4 56-57 0.0 1.834014364000499E-4 0.0 0.3239786374006881 2.1396834246672485E-4 58-59 0.0 1.834014364000499E-4 0.0 0.3830797502806042 2.1396834246672485E-4 60-61 0.0 1.834014364000499E-4 0.0 0.44821782710868857 2.1396834246672485E-4 62-63 0.0 1.834014364000499E-4 0.0 0.516748830510174 2.1396834246672485E-4 64-65 0.0 1.834014364000499E-4 0.0 0.5893757993245936 2.1396834246672485E-4 66-67 0.0 1.834014364000499E-4 0.0 0.6750548370294837 2.1396834246672485E-4 68-69 0.0 1.834014364000499E-4 0.0 0.776017327767711 2.1396834246672485E-4 70-71 0.0 1.834014364000499E-4 0.0 0.8911017291087424 2.1396834246672485E-4 72-73 0.0 1.9868488943338737E-4 0.0 1.015310351910676 2.1396834246672485E-4 74-75 0.0 2.1396834246672485E-4 0.0 1.1392133056519431 2.1396834246672485E-4 76-77 1.528345303333749E-5 2.4453524853339985E-4 0.0 1.2783538620674477 2.1396834246672485E-4 78-79 3.056690606667498E-5 2.4453524853339985E-4 0.0 1.440939235436092 2.1396834246672485E-4 80-81 3.056690606667498E-5 2.4453524853339985E-4 0.0 1.6256397653439754 2.1396834246672485E-4 82-83 3.056690606667498E-5 2.4453524853339985E-4 0.0 1.8251193943350965 2.1396834246672485E-4 84-85 3.056690606667498E-5 2.4453524853339985E-4 0.0 2.0217868679680833 2.1396834246672485E-4 86-87 3.056690606667498E-5 2.4453524853339985E-4 0.0 2.234624234910341 2.1396834246672485E-4 88-89 3.056690606667498E-5 2.4453524853339985E-4 0.0 2.4759346748537068 2.1396834246672485E-4 90-91 3.056690606667498E-5 2.4453524853339985E-4 0.0 2.7449540151465133 2.1396834246672485E-4 92-93 3.056690606667498E-5 2.4453524853339985E-4 0.0 3.0369291018953923 2.1396834246672485E-4 94-95 3.056690606667498E-5 2.4453524853339985E-4 0.0 3.335002286404574 2.1396834246672485E-4 96-97 3.056690606667498E-5 2.598187015667373E-4 0.0 3.634114745720022 2.1396834246672485E-4 98-99 3.056690606667498E-5 2.751021546000748E-4 0.0 3.954700456547309 2.1396834246672485E-4 100-101 3.056690606667498E-5 2.751021546000748E-4 0.0 4.322053533656609 2.1396834246672485E-4 102-103 3.056690606667498E-5 2.9038560763341233E-4 0.0 4.72980077713302 2.2925179550006234E-4 104-105 3.056690606667498E-5 3.056690606667498E-4 0.0 5.139641853674998 2.4453524853339985E-4 106-107 3.056690606667498E-5 3.056690606667498E-4 0.0 5.546059436737508 2.4453524853339985E-4 108-109 3.056690606667498E-5 3.056690606667498E-4 0.0 5.976441474156292 2.4453524853339985E-4 110-111 3.056690606667498E-5 3.056690606667498E-4 0.0 6.451497044791521 2.4453524853339985E-4 112-113 3.056690606667498E-5 3.056690606667498E-4 0.0 6.971088597565896 2.4453524853339985E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6530 0.0 48.58198 1 GGTATCA 3820 0.0 46.587456 1 ATCAACG 7550 0.0 39.796017 3 CAACGCA 7740 0.0 39.07302 5 TCAACGC 7815 0.0 38.393326 4 AACGCAG 7840 0.0 38.331 6 TATCAAC 8600 0.0 36.59758 2 ACGCAGA 9485 0.0 30.93174 7 GTACATG 11855 0.0 29.872847 1 CGCAGAG 9515 0.0 29.647692 8 TACATGG 11540 0.0 28.872032 2 ACATGGG 11600 0.0 26.978819 3 GCAGAGT 10965 0.0 24.044538 9 CATGGGG 6425 0.0 22.237825 4 AGAGTAC 10135 0.0 21.634594 10-11 TATACCG 210 7.121099E-5 19.84417 5 GTATCGA 275 1.3152548E-6 19.479195 1 CAGAGTA 11220 0.0 19.330349 10-11 GAGTACA 9175 0.0 18.812786 1 GAGTACT 4760 0.0 18.74605 12-13 >>END_MODULE