FastQCFastQC Report
Thu 26 May 2016
SRR1364493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364493_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3271512
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42550.13006218531370206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37560.11480929918643124No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49500.057.1113471
GTATCAA82900.049.425551
ATCAACG107200.036.244563
TATCAAC113600.035.566522
TCAACGC108600.035.5578234
CAACGCA108500.035.4210785
AACGCAG111550.034.0259366
ACGCAGA133450.028.0854237
CGCAGAG135650.027.8398488
GTACATG105450.026.526231
TACATGG106450.024.9677352
GCAGAGT153950.023.1784559
GAGTACT89550.023.01215212-13
ACATGGG105200.022.8852463
AGAGTAC141100.022.17064310-11
GTACTTT95450.021.96356214-15
AGTACTT95700.021.5954612-13
CAGAGTA150750.020.43581810-11
GTGGTAT32700.018.9685821
TGGTATC29750.018.8291572
Produced by FastQC (version 0.11.5)