##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364493_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3271512 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.768939866337032 31.0 28.0 31.0 24.0 34.0 2 29.02403139588056 31.0 28.0 33.0 25.0 34.0 3 29.088943583272812 31.0 28.0 34.0 25.0 34.0 4 32.502429152025115 35.0 32.0 37.0 26.0 37.0 5 31.672157705672483 35.0 32.0 37.0 20.0 37.0 6 31.70314735205006 35.0 32.0 37.0 19.0 37.0 7 31.22642221700547 35.0 31.0 37.0 17.0 37.0 8 31.29255280127354 35.0 32.0 37.0 17.0 37.0 9 31.924163200379517 37.0 30.0 39.0 11.0 39.0 10-11 32.067696221196805 37.0 31.0 39.0 15.5 39.0 12-13 31.8501370314399 37.0 30.5 39.0 11.5 39.0 14-15 32.746104094987274 38.0 31.0 40.0 11.0 41.0 16-17 32.7901789753484 38.0 31.0 40.0 11.0 41.0 18-19 32.64346531512035 38.0 31.0 40.0 10.0 41.0 20-21 32.54237383203852 38.0 30.5 40.0 10.0 41.0 22-23 32.494832511694895 38.0 30.5 40.0 10.0 41.0 24-25 32.31714632255667 37.0 30.0 40.0 10.0 41.0 26-27 32.37880817799231 37.0 30.0 40.0 10.0 41.0 28-29 32.26180295227405 37.0 30.0 40.0 9.0 41.0 30-31 31.80109395900122 37.0 30.0 40.0 8.0 41.0 32-33 31.732037204815388 37.0 30.0 40.0 8.0 41.0 34-35 31.558155831309804 37.0 30.0 40.0 2.0 41.0 36-37 31.533043283961668 37.0 30.0 40.0 2.0 41.0 38-39 31.31385075157909 37.0 30.0 40.0 2.0 41.0 40-41 31.077717887019823 36.5 29.5 40.0 2.0 41.0 42-43 30.966603821107796 36.0 29.0 40.0 2.0 41.0 44-45 30.882372126405162 36.0 29.0 40.0 2.0 41.0 46-47 30.76865544127608 36.0 28.5 40.0 2.0 41.0 48-49 30.532405505466585 36.0 28.0 40.0 2.0 41.0 50-51 30.218824506833535 36.0 27.5 40.0 2.0 41.0 52-53 30.125044016344738 36.0 28.0 40.0 2.0 41.0 54-55 29.676324127803902 35.0 26.5 40.0 2.0 41.0 56-57 29.68112282638731 35.0 27.0 39.5 2.0 41.0 58-59 29.449311205338695 35.0 26.0 39.0 2.0 41.0 60-61 29.184760288209244 35.0 26.0 39.0 2.0 41.0 62-63 28.926833372458972 34.5 26.0 39.0 2.0 41.0 64-65 28.49835336077019 34.0 25.0 38.5 2.0 40.0 66-67 28.12389179682055 34.0 23.5 38.0 2.0 40.0 68-69 27.942225032339785 34.0 23.5 38.0 2.0 40.0 70-71 27.986133475897383 34.0 25.5 37.0 2.0 40.0 72-73 27.75135762913295 34.0 24.5 37.0 2.0 39.5 74-75 27.412814013826022 34.0 23.5 36.0 2.0 39.0 76-77 27.28972093026099 34.0 24.5 36.0 2.0 39.0 78-79 26.90184645509477 33.0 23.0 35.5 2.0 38.5 80-81 26.655002488146152 33.0 23.0 35.0 2.0 37.0 82-83 26.509830316991042 33.0 23.0 35.0 2.0 37.0 84-85 26.122432074221337 33.0 21.0 35.0 2.0 36.0 86-87 25.971244641621368 33.0 21.0 35.0 2.0 36.0 88-89 25.883580130532916 33.0 21.0 35.0 2.0 36.0 90-91 25.70838346305928 33.0 20.0 35.0 2.0 35.0 92-93 25.718251377344785 33.0 20.5 35.0 2.0 35.0 94-95 25.71458136176789 33.0 21.0 35.0 2.0 35.0 96-97 25.601430164401048 33.0 20.0 35.0 2.0 35.0 98-99 25.486896578707338 33.0 20.0 35.0 2.0 35.0 100-101 25.380329187238196 33.0 19.5 35.0 2.0 35.0 102-103 24.250083600488093 30.5 18.5 34.0 2.0 35.0 104-105 25.185791921288995 32.0 19.0 35.0 2.0 35.0 106-107 25.29664968369366 33.0 19.0 35.0 2.0 35.0 108-109 25.366745254182163 33.0 19.0 35.0 2.0 35.0 110-111 25.301155551316945 33.0 19.0 35.0 2.0 35.0 112-113 25.24652928065066 33.0 18.5 35.0 2.0 35.0 114-115 25.19488450600212 33.0 18.0 35.0 2.0 35.0 116-117 25.113373571608477 33.0 17.5 35.0 2.0 35.0 118-119 24.992980615690847 33.0 13.0 35.0 2.0 35.0 120-121 24.83082898671929 33.0 4.5 35.0 2.0 35.0 122-123 24.531195055986345 33.0 2.0 35.0 2.0 35.0 124-125 23.768400207610426 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2604304182777462 1101 2 1.2776821229772182 1101 3 1.171865076177621 1101 4 1.215793492238582 1101 5 1.4256311270690283 1101 6 1.4512646874690276 1101 7 1.9911200013854042 1101 8 1.801669481270757 1101 9 2.3830487399027582 1101 10-11 2.115718671767972 1101 12-13 2.238214385775592 1101 14-15 2.3013281599055446 1101 16-17 2.1857959835248053 1101 18-19 2.228852137085937 1101 20-21 2.5235318394640487 1101 22-23 2.5364258587769584 1101 24-25 2.6044462063698575 1101 26-27 2.4083693731802995 1101 28-29 2.4503613763673826 1101 30-31 2.852784834131647 1101 32-33 2.751833191967801 1101 34-35 2.713847418356945 1101 36-37 2.62648242915386 1101 38-39 2.765869891423666 1101 40-41 2.9633757329471067 1101 42-43 2.880959592228024 1101 44-45 2.741724587860503 1101 46-47 2.620791432506749 1101 48-49 2.589317162080789 1101 50-51 2.8302683555121924 1101 52-53 2.6440448504337226 1101 54-55 3.031197996463959 1101 56-57 2.8070024289985724 1101 58-59 2.8476797943099825 1101 60-61 3.014985981120894 1101 62-63 2.943280981009625 1101 64-65 3.169408973815692 1101 66-67 3.3215605286390613 1101 68-69 3.2182296527932728 1101 70-71 2.857121152906256 1101 72-73 3.0582249534420107 1101 74-75 3.1322978397336634 1101 76-77 2.8565143171314773 1101 78-79 2.8908852510322625 1101 80-81 2.980424915134069 1101 82-83 2.8802659240149495 1101 84-85 3.1576687020052496 1101 86-87 3.0138009515620574 1101 88-89 2.8859339885029343 1101 90-91 2.8263425934239947 1101 92-93 2.730512715847592 1101 94-95 2.6772737584931896 1101 96-97 2.600464766198016 1101 98-99 2.710360119198544 1101 100-101 2.810428070743203 1101 102-103 2.5254397306600502 1101 104-105 2.58645027948727 1101 106-107 2.712721484538477 1101 108-109 2.6102076843433544 1101 110-111 2.6173208029424977 1101 112-113 2.4460676983819596 1101 114-115 2.4609966913477983 1101 116-117 2.5276483244213566 1101 118-119 2.432171140263467 1101 120-121 2.378730307170425 1101 122-123 2.5005077520482466 1101 124-125 2.36469322420756 1102 1 0.8109107325393516 1102 2 -1.3020016993136636 1102 3 0.36797655350031633 1102 4 0.8302256738017704 1102 5 1.2384865399669458 1102 6 0.865134000960289 1102 7 1.6609486599237329 1102 8 1.441027199088765 1102 9 2.289441186554537 1102 10-11 1.9410024716435785 1102 12-13 1.9726125194242528 1102 14-15 1.9611745859972345 1102 16-17 2.030094283947257 1102 18-19 2.1365878567216328 1102 20-21 2.335114905753642 1102 22-23 2.263846473955148 1102 24-25 2.4651119354220583 1102 26-27 2.171911852595869 1102 28-29 2.165628523595636 1102 30-31 2.504911626344672 1102 32-33 2.190996097372487 1102 34-35 2.4335851401373283 1102 36-37 1.841088508066555 1102 38-39 1.799810757408185 1102 40-41 2.012529891579028 1102 42-43 1.9585987856062275 1102 44-45 1.8688904050377886 1102 46-47 1.6049118484727813 1102 48-49 1.7294384570847825 1102 50-51 1.8510838392850708 1102 52-53 1.7171563897173385 1102 54-55 2.0482014194141343 1102 56-57 1.628296095499401 1102 58-59 1.6578070068419137 1102 60-61 1.6649166102767339 1102 62-63 2.016637646663572 1102 64-65 2.076697169011325 1102 66-67 2.1267448610661503 1102 68-69 2.0553271606626957 1102 70-71 1.4561644262286215 1102 72-73 1.6969800655431087 1102 74-75 1.9987845756715927 1102 76-77 1.93316543889744 1102 78-79 1.78724009417612 1102 80-81 1.9539877147365807 1102 82-83 1.611037749422625 1102 84-85 1.5733709554561166 1102 86-87 1.6129683257026457 1102 88-89 1.7336876514214836 1102 90-91 1.4861025215545354 1102 92-93 1.5702021686415755 1102 94-95 1.6135879586597568 1102 96-97 1.659110042159707 1102 98-99 1.4713530240226689 1102 100-101 1.2960402836565343 1102 102-103 1.0574708563883881 1102 104-105 1.425138513499597 1102 106-107 1.6727660163443439 1102 108-109 1.5769595179829778 1102 110-111 1.4280247010912426 1102 112-113 1.4710361907348606 1102 114-115 1.7157467246062303 1102 116-117 1.3169601345366573 1102 118-119 0.9201294720673054 1102 120-121 1.2641385046518465 1102 122-123 1.5926506000792067 1102 124-125 1.5016680338404562 1103 1 1.068952404617157 1103 2 0.9957876435581632 1103 3 0.9300598100330078 1103 4 1.0045187667322821 1103 5 1.3776792742898714 1103 6 1.4094137748148015 1103 7 1.7336615824831583 1103 8 1.5432933028532432 1103 9 2.4036725203419174 1103 10-11 2.0875695204493567 1103 12-13 2.0948961680140243 1103 14-15 1.9445471570320407 1103 16-17 1.9585752726785728 1103 18-19 2.113978694197634 1103 20-21 2.3329985542650604 1103 22-23 2.211875976762961 1103 24-25 2.5211651907017796 1103 26-27 1.9708262228661866 1103 28-29 2.013872460980636 1103 30-31 2.6165161985897427 1103 32-33 2.4157584756936608 1103 34-35 2.5540537144895232 1103 36-37 2.49090950462125 1103 38-39 2.378133179337116 1103 40-41 2.613325928202279 1103 42-43 2.6571373762888015 1103 44-45 2.483307611071865 1103 46-47 2.335065387077506 1103 48-49 2.397337149707681 1103 50-51 2.6116816587540406 1103 52-53 2.3640154931676918 1103 54-55 2.770792812505647 1103 56-57 2.469364485974044 1103 58-59 2.620736487300011 1103 60-61 2.5556297198347018 1103 62-63 2.5494092726636026 1103 64-65 2.634884551741397 1103 66-67 2.8292499397025033 1103 68-69 2.9021987905345945 1103 70-71 2.6664978980479574 1103 72-73 2.75740263263749 1103 74-75 2.809377540621707 1103 76-77 2.630995472235284 1103 78-79 2.7351603458249762 1103 80-81 2.7331773783396436 1103 82-83 2.5930843708244105 1103 84-85 2.772415516282784 1103 86-87 2.6577878423962815 1103 88-89 2.5914780637891894 1103 90-91 2.617387793127161 1103 92-93 2.4341676791622966 1103 94-95 2.2775998695008823 1103 96-97 2.183482991435632 1103 98-99 2.37446041884235 1103 100-101 2.341742216068443 1103 102-103 2.1554650488559908 1103 104-105 2.2105061154919916 1103 106-107 2.2000377695673734 1103 108-109 2.241378398777087 1103 110-111 2.185711604800769 1103 112-113 2.231094087809531 1103 114-115 2.045761845763206 1103 116-117 2.170714163020115 1103 118-119 2.2253231477816584 1103 120-121 2.1973689724609677 1103 122-123 2.2728441860159734 1103 124-125 2.136998079420696 1104 1 0.8233443469560271 1104 2 0.7177106103154642 1104 3 0.7318784600579242 1104 4 0.8079111670117811 1104 5 1.071398008997658 1104 6 1.1251773528044353 1104 7 1.5183689703843015 1104 8 1.2905046513051772 1104 9 1.861869233888708 1104 10-11 1.3659663580414687 1104 12-13 1.5464638979821075 1104 14-15 1.5571367664413316 1104 16-17 1.3890006102820251 1104 18-19 1.229522014459178 1104 20-21 1.632536288498457 1104 22-23 1.7209171570183202 1104 24-25 1.8424252528690275 1104 26-27 1.7583757967470675 1104 28-29 1.6069581806367097 1104 30-31 2.122051372144753 1104 32-33 2.1229819095902904 1104 34-35 2.0104626335377382 1104 36-37 1.9575998390325822 1104 38-39 2.103557727946498 1104 40-41 2.2392298436819402 1104 42-43 2.2182433777042547 1104 44-45 1.9679912481325914 1104 46-47 2.106358348926335 1104 48-49 1.8069500889112646 1104 50-51 2.249151628991637 1104 52-53 2.071945594026811 1104 54-55 2.391322531656165 1104 56-57 2.2404538424814504 1104 58-59 2.38923157397344 1104 60-61 2.401089495221928 1104 62-63 2.5024512872833036 1104 64-65 2.650399378473061 1104 66-67 2.6099994254852774 1104 68-69 2.722984396789908 1104 70-71 2.5196242839291294 1104 72-73 2.656270014824969 1104 74-75 2.7154407232168687 1104 76-77 2.4732484167536697 1104 78-79 2.5348511735757278 1104 80-81 2.4129553481793806 1104 82-83 2.369858192774 1104 84-85 2.667878632118832 1104 86-87 2.586429872671218 1104 88-89 2.4304324085788522 1104 90-91 2.490902384033113 1104 92-93 2.238960824361886 1104 94-95 2.219125967255877 1104 96-97 2.2799072279374144 1104 98-99 2.3469737912323474 1104 100-101 2.3146272445507527 1104 102-103 2.166925063060372 1104 104-105 1.9815041082312526 1104 106-107 2.1638847962064567 1104 108-109 2.1414654187153275 1104 110-111 2.2533582797952896 1104 112-113 2.2104813373163736 1104 114-115 2.088137963099708 1104 116-117 2.1403133989049117 1104 118-119 2.0903612251589188 1104 120-121 2.109937336366471 1104 122-123 2.222685259177535 1104 124-125 2.026848960788147 1105 1 0.7226046093326559 1105 2 0.7880685003641936 1105 3 0.6644628568017907 1105 4 0.9083038070346277 1105 5 1.1154006098419522 1105 6 1.2068366502447105 1105 7 1.471277804106201 1105 8 1.4230691672234101 1105 9 2.005542381051054 1105 10-11 1.6260894934914703 1105 12-13 1.8149728586799085 1105 14-15 1.7828592874205071 1105 16-17 1.6568309587268004 1105 18-19 1.96107859011137 1105 20-21 2.1391880709363704 1105 22-23 2.038601754631415 1105 24-25 2.1583182512996757 1105 26-27 1.902690923667663 1105 28-29 1.9687292002742396 1105 30-31 2.2424160106153366 1105 32-33 2.1348256412740447 1105 34-35 2.2023162453132166 1105 36-37 1.9999420390633489 1105 38-39 2.1514821421030526 1105 40-41 2.259322609976927 1105 42-43 2.1413492578040234 1105 44-45 2.1012549231748494 1105 46-47 2.097076001490933 1105 48-49 2.1973729975539698 1105 50-51 2.185666196031992 1105 52-53 2.1567261308241825 1105 54-55 2.3285123807227457 1105 56-57 2.1589479239772515 1105 58-59 2.207207220841717 1105 60-61 2.020157670116891 1105 62-63 2.012938862646962 1105 64-65 2.422006769616118 1105 66-67 2.630042741054048 1105 68-69 2.5200492354259687 1105 70-71 2.3630525400363886 1105 72-73 2.637283135164534 1105 74-75 2.5911182044494687 1105 76-77 2.512298216336852 1105 78-79 2.499884923225114 1105 80-81 2.474634667194497 1105 82-83 2.442693457387538 1105 84-85 2.6519792497062475 1105 86-87 2.68581332830556 1105 88-89 2.427589525914428 1105 90-91 2.513916324739494 1105 92-93 2.2988467219949875 1105 94-95 2.1848030790218083 1105 96-97 2.2637996120983033 1105 98-99 2.3552659302885424 1105 100-101 2.2344380223208233 1105 102-103 2.027727677879053 1105 104-105 1.7549480880357535 1105 106-107 2.055654938674813 1105 108-109 2.0613143370073956 1105 110-111 2.173686939249567 1105 112-113 2.146370135891189 1105 114-115 2.038706715569102 1105 116-117 2.0318481006221525 1105 118-119 2.094800543148576 1105 120-121 2.15455119091423 1105 122-123 2.285207125572356 1105 124-125 2.09913831954481 1106 1 0.4906084712118428 1106 2 0.4556289106302245 1106 3 0.5128336753794613 1106 4 0.3545740447669061 1106 5 0.619533954119877 1106 6 0.8926874363012942 1106 7 1.0076814877448115 1106 8 0.6627063929244521 1106 9 1.4452624729856716 1106 10-11 1.1240063067375559 1106 12-13 1.077389992446335 1106 14-15 1.2261996956539676 1106 16-17 1.2321461044853805 1106 18-19 1.2166888686513602 1106 20-21 1.1139930225847081 1106 22-23 1.0359215987490842 1106 24-25 0.7895467041245467 1106 26-27 0.874002496797111 1106 28-29 0.9836899756207558 1106 30-31 1.3077361987060456 1106 32-33 1.1904891770042418 1106 34-35 1.0699776976612263 1106 36-37 1.1656163630390495 1106 38-39 1.3806554537035431 1106 40-41 1.518411022775858 1106 42-43 1.5233902492281501 1106 44-45 1.4703674140869225 1106 46-47 1.4496895125957963 1106 48-49 1.5279163381576453 1106 50-51 1.7354592258872863 1106 52-53 1.4480853715818611 1106 54-55 1.8447954979002894 1106 56-57 1.6217256431232627 1106 58-59 1.5429332189498695 1106 60-61 1.777815370031682 1106 62-63 1.6893376212169713 1106 64-65 1.7985257233126575 1106 66-67 1.9387491678776954 1106 68-69 1.8338963980392258 1106 70-71 1.7430670220833377 1106 72-73 1.7305482015747877 1106 74-75 2.009987666678885 1106 76-77 1.6156127679601724 1106 78-79 1.4829598858962179 1106 80-81 1.6348678652838835 1106 82-83 1.4730759515048177 1106 84-85 1.766780839799825 1106 86-87 1.735400658371148 1106 88-89 1.3942151503427667 1106 90-91 1.591931854340718 1106 92-93 1.5447133777170556 1106 94-95 1.3590994000737382 1106 96-97 1.3441241584838473 1106 98-99 1.3372755537162355 1106 100-101 1.0586081873497086 1106 102-103 0.893793662071932 1106 104-105 1.1341415298769455 1106 106-107 1.1487487025998604 1106 108-109 1.0764465125587321 1106 110-111 1.0837800074109545 1106 112-113 0.8280296299381078 1106 114-115 0.9509216674519649 1106 116-117 1.1593267586582243 1106 118-119 0.9192821405906706 1106 120-121 0.897425672023072 1106 122-123 1.1116173173373305 1106 124-125 1.036058255909797 1107 1 0.5186884968328513 1107 2 0.5762278017209184 1107 3 0.4197443421261937 1107 4 0.5375811105608577 1107 5 0.5456443646565212 1107 6 0.8015366926915277 1107 7 0.8695091864577584 1107 8 0.9689254925861732 1107 9 1.6352492621527865 1107 10-11 1.1476461914430232 1107 12-13 1.42237688169552 1107 14-15 1.171107019659651 1107 16-17 1.2517626900855703 1107 18-19 1.3339735420835765 1107 20-21 1.480019164020682 1107 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-8.712279338776046 2216 82-83 -8.923003099212725 2216 84-85 -8.918634839785764 2216 86-87 -9.312249035765326 2216 88-89 -8.9696687373353 2216 90-91 -8.344794405701187 2216 92-93 -7.7873441204140725 2216 94-95 -7.461046793215161 2216 96-97 -7.7751352411602745 2216 98-99 -7.713312220165864 2216 100-101 -8.108648095750407 2216 102-103 -7.942885481299623 2216 104-105 -7.850379196193579 2216 106-107 -8.055144345190655 2216 108-109 -8.161341583506633 2216 110-111 -7.772663131454248 2216 112-113 -7.793842244305125 2216 114-115 -7.579892108514848 2216 116-117 -7.547688624013372 2216 118-119 -7.745428789423965 2216 120-121 -7.6885688319094605 2216 122-123 -7.688421902684361 2216 124-125 -7.57039857047975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 171872.0 3 56145.0 4 26736.0 5 21356.0 6 21019.0 7 24445.0 8 27360.0 9 29097.0 10 31210.0 11 33361.0 12 35622.0 13 36632.0 14 37132.0 15 30896.0 16 23596.0 17 20814.0 18 18198.0 19 16999.0 20 16862.0 21 17890.0 22 22146.0 23 30608.0 24 41834.0 25 55310.0 26 67824.0 27 76251.0 28 87438.0 29 98959.0 30 110567.0 31 126476.0 32 147393.0 33 175580.0 34 211682.0 35 267082.0 36 377309.0 37 496537.0 38 180513.0 39 761.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.93395947001128 21.017924937031406 15.11784734308031 23.930268249877003 2 15.887876749155664 21.907610162533658 38.188883594013404 24.01562949429728 3 18.916941157483144 25.74320375410514 29.721700547025353 25.618154541386367 4 13.256343840131157 18.572281149036645 37.008237369504556 31.163137641327644 5 13.304001330271753 38.601172791051965 34.457675839183835 13.637150039492443 6 30.842810104697893 38.24740761752684 17.48044021289265 13.429342064882615 7 27.087986213206065 32.66755871496122 21.871980469523994 18.372474602308724 8 25.137031439896905 36.42312789927104 19.912016217577683 18.527824443254374 9 25.241020054335735 16.524469419644493 20.2151482250409 38.01936230097888 10-11 24.301581202733793 27.386147687129164 27.803219749246296 20.509051360890748 12-13 25.17469820409543 24.529993742929467 28.158941818414128 22.136366234560974 14-15 22.306478445301508 25.92750578671421 26.008600023995093 25.757415743989192 16-17 21.99352631702828 28.983028361872094 26.57509829892349 22.44834702217614 18-19 21.89379887708041 27.907544371463366 28.536680124421054 21.66197662703517 20-21 22.707037545651083 27.134797071911485 28.557116295917595 21.601049086519833 22-23 22.29863027935454 26.869220870128718 28.44068568563979 22.391463164876953 24-25 22.15250816245993 27.09809838603231 28.350123907991094 22.399269543516663 26-27 22.06784198550099 27.25544385332745 28.373865469053577 22.302848692117983 28-29 21.99850523625826 27.29521125382861 28.490227485312186 22.216056024600938 30-31 22.220374611836245 27.080021262463344 28.279060966965236 22.42054315873517 32-33 22.12097777478901 27.002103004423034 28.399852056084196 22.477067164703758 34-35 22.31251087045233 27.188159009232205 28.261861669463055 22.23746845085241 36-37 22.48360033495497 26.947934492735637 28.28474351628443 22.283721656024962 38-39 22.232261795666695 27.175532431931792 28.244354726794878 22.34785104560663 40-41 22.400638367169023 27.11518348853108 28.105196698183065 22.378981446116832 42-43 22.386066909827854 27.088033978375314 27.955145854682534 22.570753257114298 44-45 22.383312894087208 27.130913677095776 27.985678670597196 22.500094758219817 46-47 22.504660693276403 27.322941798417794 27.611050435746932 22.56134707255887 48-49 22.561742485086786 27.418473081937815 27.40396908279246 22.615815350182935 50-51 22.489423498691803 27.46737762241504 27.276449432849404 22.76674944604375 52-53 22.67810236280764 27.48077684604711 27.16437865628125 22.676742134864003 54-55 22.579299431777315 27.32618553167004 27.174940048145128 22.919574988407522 56-57 22.44108863096127 27.515391623453866 27.155450085870303 22.888069659714564 58-59 22.529353399895825 27.38440207463705 27.216498059612803 22.86974646585432 60-61 22.69615700630167 27.3209298941896 27.274468196968254 22.708444902540474 62-63 22.50056205096102 27.386210089223585 27.33470467144969 22.778523188365707 64-65 22.421640275589393 27.268615251367674 27.23506571671837 23.074678756324563 66-67 22.333122938482138 27.398516593976467 27.059881799683303 23.20847866785809 68-69 22.46791265431561 27.2823671136535 27.316648433222955 22.933071798807934 70-71 22.398582029854158 27.135353668739455 27.351023418760622 23.11504088264576 72-73 22.478884380138204 27.227163189671007 27.066533529969444 23.227418900221345 74-75 22.394609323606876 27.392955573108612 27.042397149557733 23.170037953726776 76-77 22.517167437730308 27.165374428197854 27.228205798337434 23.089252335734407 78-79 22.483754249999464 27.318658792940546 27.090351710285677 23.10723524677431 80-81 22.366983803653937 27.31046631532019 27.128134581299552 23.19441529972632 82-83 22.508703931823533 27.357917292015006 27.094736069888214 23.03864270627325 84-85 22.441033754581024 27.356162434397678 26.927461511292254 23.27534229972904 86-87 22.364010559057853 27.18072482870264 27.265364798575824 23.189899813663683 88-89 22.45220007064093 27.090551981988547 27.215877733419596 23.241370213950926 90-91 22.43742059270302 27.301804812374492 27.194254873944047 23.066519720978444 92-93 22.339226405004155 27.290959441261425 27.119414215236375 23.250399938498038 94-95 22.296766277359723 27.112701135212813 27.282691130227132 23.307841457200336 96-97 22.28275438972418 27.142775047249735 27.18935993766673 23.38511062535936 98-99 22.087248954000476 27.043137851855654 27.2085200971294 23.66109309701447 100-101 22.11843472139457 27.053400729913957 27.241985015045934 23.586179533645538 102-103 22.12341013919263 26.90622436648195 27.29763820492509 23.672727289400328 104-105 22.089335852252866 26.8708609499257 27.381741481032375 23.658061716789057 106-107 21.823665245763387 26.88643365197819 27.389427605382892 23.900473496875527 108-109 21.995701070330785 26.845522804134603 27.16163046322312 23.997145662311496 110-111 21.822841331887314 26.892231350190798 27.299016233492324 23.985911084429564 112-113 21.954826038202256 26.877295290536463 27.282918567993942 23.884960103267346 114-115 21.897857854302423 26.927004185682478 27.218673826256655 23.956464133758438 116-117 21.76690777842172 26.829093092123763 27.328006132944033 24.075992996510482 118-119 21.68348090399967 26.811857678634173 27.2804218636591 24.224239553707054 120-121 21.688986895504097 26.806634826404135 27.391515862536757 24.11286241555501 122-123 21.712770892095556 26.812064560944464 27.263862790034793 24.211301756925184 124-125 21.67119011776869 26.770790871050536 27.18319755337772 24.374821457803055 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 72.0 1 64.5 2 85.0 3 136.5 4 128.0 5 116.5 6 198.5 7 433.5 8 751.5 9 929.0 10 905.0 11 827.5 12 852.0 13 1001.0 14 1201.5 15 1436.5 16 1682.5 17 2006.5 18 2396.0 19 2789.0 20 3298.0 21 4035.0 22 5003.0 23 6275.5 24 8059.5 25 10812.0 26 14997.5 27 20387.5 28 26145.0 29 32724.0 30 40012.5 31 48066.0 32 55490.0 33 62266.5 34 69720.0 35 76659.0 36 84430.0 37 92064.5 38 96476.0 39 99887.0 40 103605.5 41 107319.0 42 111001.0 43 114412.5 44 118131.0 45 121818.0 46 123423.5 47 123331.0 48 124416.0 49 124202.5 50 120496.5 51 116144.0 52 113858.5 53 111585.0 54 106546.0 55 98405.5 56 90006.0 57 82109.0 58 75189.5 59 67988.0 60 57965.0 61 46409.5 62 35816.0 63 27175.5 64 19664.5 65 14032.0 66 10336.0 67 7942.5 68 6055.5 69 4667.0 70 3594.0 71 2578.5 72 1895.0 73 1529.5 74 1128.0 75 749.0 76 497.5 77 282.0 78 154.5 79 83.0 80 62.5 81 48.0 82 30.5 83 17.0 84 7.0 85 5.5 86 5.0 87 3.0 88 2.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09830316991042674 2 1.5283453033337492E-4 3 0.0 4 0.17682955159571476 5 0.0 6 1.2226762426669992E-4 7 0.03472400529174278 8 0.0 9 0.0 10-11 4.585035910001247E-4 12-13 3.9736977886677474E-4 14-15 2.9038560763341233E-4 16-17 0.0034540603855342726 18-19 0.0012226762426669994 20-21 0.0028885726233007854 22-23 0.0012838100548003492 24-25 0.004065398506867772 26-27 0.0035457611037342978 28-29 0.002812155358134098 30-31 5.807712152668247E-4 32-33 6.113381213334996E-5 34-35 8.864402759335744E-4 36-37 8.405899168335621E-4 38-39 4.279366849334497E-4 40-41 0.0012379596957003366 42-43 5.960546683001621E-4 44-45 8.558733698668994E-4 46-47 9.170071820002494E-5 48-49 1.528345303333749E-5 50-51 0.035839697363176416 52-53 4.585035910001247E-5 54-55 0.13165166442916915 56-57 2.5981870156673736E-4 58-59 0.0 60-61 0.0 62-63 3.5151941976676226E-4 64-65 0.05257507843468097 66-67 0.044933351918012225 68-69 0.001558912209400424 70-71 0.0022925179550006237 72-73 0.06695680773905155 74-75 0.004890704970667997 76-77 0.001742313645800474 78-79 0.0012532431487336743 80-81 7.641726516668746E-5 82-83 6.113381213334996E-5 84-85 1.528345303333749E-5 86-87 2.4453524853339985E-4 88-89 0.0011462589775003119 90-91 2.5981870156673736E-4 92-93 0.002185533783767261 94-95 0.004371067567534522 96-97 0.0019868488943338737 98-99 0.0 100-101 9.322906350335869E-4 102-103 0.0011768258835669868 104-105 1.0698417123336243E-4 106-107 4.585035910001247E-5 108-109 0.0 110-111 1.2226762426669992E-4 112-113 1.0698417123336243E-4 114-115 7.641726516668746E-5 116-117 0.0 118-119 8.71156822900237E-4 120-121 0.002368935220167311 122-123 9.017237289669119E-4 124-125 0.006923404224101884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3271512.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.75050038883635 #Duplication Level Percentage of deduplicated Percentage of total 1 80.4019456312553 49.64860374966029 2 10.913052084874742 13.47772854020898 3 3.696641612378752 6.8480840796774824 4 1.6664714499436617 4.116217836709231 5 0.9143318324550691 2.8230224087771103 6 0.530094562791593 1.964016270346938 7 0.3468646067099576 1.4993344122061765 8 0.24360062786645797 1.2033968532630794 9 0.1984416819126985 1.1028485840500286 >10 0.9928859497211435 11.134507053284329 >50 0.0686108081477109 2.9051984382052525 >100 0.026356516355617213 2.716281586342145 >500 4.0150605185353046E-4 0.1442034470422433 >1k 3.011295354640494E-4 0.41655674022665007 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4255 0.13006218531370206 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3756 0.11480929918643124 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 6.113381213334996E-5 0.0 0.0 0.0 6 0.0 6.113381213334996E-5 0.0 0.0 0.0 7 0.0 6.113381213334996E-5 0.0 0.0 0.0 8 0.0 6.113381213334996E-5 0.0 0.0 0.0 9 0.0 6.113381213334996E-5 0.0 0.0 0.0 10-11 0.0 6.113381213334996E-5 0.0 1.528345303333749E-5 0.0 12-13 0.0 6.113381213334996E-5 0.0 6.113381213334996E-5 0.0 14-15 0.0 6.113381213334996E-5 0.0 6.113381213334996E-5 0.0 16-17 0.0 6.113381213334996E-5 0.0 9.170071820002494E-5 0.0 18-19 0.0 9.170071820002494E-5 0.0 1.2226762426669992E-4 0.0 20-21 0.0 9.170071820002494E-5 0.0 2.1396834246672485E-4 0.0 22-23 0.0 9.170071820002494E-5 0.0 3.668028728000998E-4 0.0 24-25 0.0 9.170071820002494E-5 0.0 4.5850359100012473E-4 1.528345303333749E-5 26-27 0.0 9.170071820002494E-5 0.0 5.654877622334872E-4 3.056690606667498E-5 28-29 0.0 9.170071820002494E-5 0.0 9.475740880669245E-4 3.056690606667498E-5 30-31 0.0 9.170071820002494E-5 0.0 0.0020632661595005614 3.056690606667498E-5 32-33 0.0 9.170071820002494E-5 0.0 0.005028256047968034 3.056690606667498E-5 34-35 0.0 9.170071820002494E-5 0.0 0.009261772538202519 3.056690606667498E-5 36-37 0.0 9.170071820002494E-5 0.0 0.017438419911038075 3.056690606667498E-5 38-39 0.0 9.170071820002494E-5 0.0 0.031269944906208505 3.056690606667498E-5 40-41 0.0 9.170071820002494E-5 0.0 0.048586097192979884 3.056690606667498E-5 42-43 0.0 9.170071820002494E-5 0.0 0.06668170558445147 3.056690606667498E-5 44-45 0.0 9.170071820002494E-5 0.0 0.08847590960999073 3.056690606667498E-5 46-47 0.0 9.170071820002494E-5 0.0 0.11613895960033159 3.056690606667498E-5 48-49 0.0 9.170071820002494E-5 0.0 0.14725606997620672 3.056690606667498E-5 50-51 0.0 9.170071820002494E-5 0.0 0.18387522344408336 4.585035910001247E-5 52-53 0.0 9.170071820002494E-5 0.0 0.22228254091686045 6.113381213334996E-5 54-55 0.0 9.170071820002494E-5 0.0 0.2645871389131386 6.113381213334996E-5 56-57 0.0 1.2226762426669992E-4 0.0 0.31248548071961835 6.113381213334996E-5 58-59 0.0 1.2226762426669992E-4 0.0 0.3688202886005003 6.113381213334996E-5 60-61 0.0 1.2226762426669992E-4 0.0 0.43097809208708393 6.113381213334996E-5 62-63 0.0 1.681179833667124E-4 0.0 0.4954589804347348 6.113381213334996E-5 64-65 0.0 1.834014364000499E-4 0.0 0.5628284414056864 9.170071820002494E-5 66-67 0.0 1.834014364000499E-4 0.0 0.6431735540019416 9.170071820002494E-5 68-69 0.0 1.834014364000499E-4 0.0 0.738740985819401 9.170071820002494E-5 70-71 0.0 1.834014364000499E-4 0.0 0.8467797153120636 9.170071820002494E-5 72-73 0.0 1.834014364000499E-4 0.0 0.9628881080063285 9.170071820002494E-5 74-75 0.0 1.834014364000499E-4 0.0 1.0774070215851266 9.170071820002494E-5 76-77 3.056690606667498E-5 2.1396834246672485E-4 0.0 1.2063076644682948 9.170071820002494E-5 78-79 3.056690606667498E-5 2.1396834246672485E-4 0.0 1.3573234638907024 9.170071820002494E-5 80-81 3.056690606667498E-5 2.1396834246672485E-4 0.0 1.5286204054883492 9.170071820002494E-5 82-83 3.056690606667498E-5 2.1396834246672485E-4 0.0 1.7145741785449662 9.170071820002494E-5 84-85 3.056690606667498E-5 2.1396834246672485E-4 0.0 1.8986480868784832 9.170071820002494E-5 86-87 3.056690606667498E-5 2.1396834246672485E-4 0.0 2.0984945187424042 9.170071820002494E-5 88-89 3.056690606667498E-5 2.1396834246672485E-4 0.0 2.325407945928366 9.170071820002494E-5 90-91 3.056690606667498E-5 2.1396834246672485E-4 0.0 2.579755171309168 9.170071820002494E-5 92-93 3.056690606667498E-5 2.1396834246672485E-4 0.0 2.8555756482018104 1.2226762426669992E-4 94-95 3.056690606667498E-5 2.2925179550006234E-4 0.0 3.141009417052421 1.2226762426669992E-4 96-97 3.056690606667498E-5 2.4453524853339985E-4 0.0 3.4279715312063654 1.2226762426669992E-4 98-99 3.056690606667498E-5 2.4453524853339985E-4 0.0 3.7371405026177498 1.2226762426669992E-4 100-101 3.056690606667498E-5 2.598187015667373E-4 0.0 4.092847588515647 1.2226762426669992E-4 102-103 3.056690606667498E-5 2.9038560763341233E-4 0.0 4.492219499729789 1.2226762426669992E-4 104-105 3.056690606667498E-5 3.056690606667498E-4 0.0 4.89347127566703 1.2226762426669992E-4 106-107 3.056690606667498E-5 3.056690606667498E-4 0.0 5.2935156588146395 1.2226762426669992E-4 108-109 3.056690606667498E-5 3.056690606667498E-4 0.0 5.713642499248055 1.2226762426669992E-4 110-111 3.056690606667498E-5 3.362359667334248E-4 0.0 6.17709792903098 1.2226762426669992E-4 112-113 3.056690606667498E-5 3.362359667334248E-4 0.0 6.685318592748551 1.2226762426669992E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4950 0.0 57.111347 1 GTATCAA 8290 0.0 49.42555 1 ATCAACG 10720 0.0 36.24456 3 TATCAAC 11360 0.0 35.56652 2 TCAACGC 10860 0.0 35.557823 4 CAACGCA 10850 0.0 35.421078 5 AACGCAG 11155 0.0 34.025936 6 ACGCAGA 13345 0.0 28.085423 7 CGCAGAG 13565 0.0 27.839848 8 GTACATG 10545 0.0 26.52623 1 TACATGG 10645 0.0 24.967735 2 GCAGAGT 15395 0.0 23.178455 9 GAGTACT 8955 0.0 23.012152 12-13 ACATGGG 10520 0.0 22.885246 3 AGAGTAC 14110 0.0 22.170643 10-11 GTACTTT 9545 0.0 21.963562 14-15 AGTACTT 9570 0.0 21.59546 12-13 CAGAGTA 15075 0.0 20.435818 10-11 GTGGTAT 3270 0.0 18.968582 1 TGGTATC 2975 0.0 18.829157 2 >>END_MODULE