FastQCFastQC Report
Thu 26 May 2016
SRR1364492_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364492_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2715281
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71350.052.3075681
GGTATCA43100.048.199121
CAACGCA90600.039.3998225
TCAACGC89950.039.2870254
ATCAACG90350.039.1806563
AACGCAG90850.038.7473346
TATCAAC101050.037.1507762
GTACATG125450.036.86731
TACATGG130400.034.628932
ACGCAGA104950.033.088837
CGCAGAG107200.031.8410368
ACATGGG131750.031.6555793
GCAGAGT121850.026.4977559
GTATAGG6550.026.3540691
CATGGGG77400.025.4848844
ACGCATA2901.4370016E-1024.6496418
AGAGTAC104500.024.18111810-11
GAGTACT47550.023.39300212-13
CATGGGA58100.023.3859144
CAGAGTA115000.022.80148110-11
Produced by FastQC (version 0.11.5)