##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364492_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2715281 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.666011731382497 31.0 26.0 31.0 10.0 34.0 2 26.94159425856845 31.0 26.0 31.0 10.0 34.0 3 26.98045579812918 31.0 26.0 31.0 10.0 34.0 4 29.834698876469876 35.0 28.0 37.0 10.0 37.0 5 30.546020835412616 35.0 30.0 37.0 12.0 37.0 6 30.65715445289088 35.0 31.0 37.0 11.0 37.0 7 30.467094565903125 35.0 30.0 37.0 10.0 37.0 8 30.062999741094938 35.0 30.0 37.0 10.0 37.0 9 30.765073301805597 35.0 29.0 39.0 8.0 39.0 10-11 30.82120119427787 35.0 29.5 39.0 8.0 39.0 12-13 30.677793753206387 35.0 29.0 39.0 8.0 39.0 14-15 31.395056902029662 37.0 29.0 40.0 7.5 41.0 16-17 31.661033425269796 37.0 30.0 40.0 7.0 41.0 18-19 31.609406540243903 37.0 30.0 40.0 2.0 41.0 20-21 31.37531861343264 37.0 29.0 40.0 2.0 41.0 22-23 31.428916381030177 37.0 29.0 40.0 2.0 41.0 24-25 31.435017038752157 37.0 29.5 40.0 2.0 41.0 26-27 31.54436483737779 37.0 30.0 40.0 2.0 41.0 28-29 31.485029357919124 37.0 30.0 40.0 2.0 41.0 30-31 31.124983565236896 37.0 29.0 40.0 2.0 41.0 32-33 31.12184153315992 37.0 29.5 40.0 2.0 41.0 34-35 30.82482623345429 36.0 29.0 40.0 2.0 41.0 36-37 30.586615528926842 36.0 28.0 40.0 2.0 41.0 38-39 30.417100476893552 36.0 28.0 40.0 2.0 41.0 40-41 30.425175884190253 36.0 28.0 40.0 2.0 41.0 42-43 30.248969075392196 36.0 28.0 40.0 2.0 41.0 44-45 29.88546047352005 35.0 27.0 40.0 2.0 41.0 46-47 29.59107234205226 35.0 26.5 40.0 2.0 41.0 48-49 29.29401689917176 35.0 26.0 39.5 2.0 41.0 50-51 29.23879775242415 35.0 26.0 39.0 2.0 41.0 52-53 28.98216556592117 35.0 25.5 39.0 2.0 41.0 54-55 28.830716599865724 34.5 25.5 39.0 2.0 41.0 56-57 28.363170331173826 34.0 24.0 39.0 2.0 40.0 58-59 28.097770359679167 34.0 23.0 38.5 2.0 40.0 60-61 27.642655769329217 33.5 22.5 38.0 2.0 40.0 62-63 27.550207879037195 33.0 22.5 38.0 2.0 40.0 64-65 27.3177035820602 33.0 22.5 37.0 2.0 40.0 66-67 27.048512842685525 33.0 22.0 37.0 2.0 39.5 68-69 26.842176371432643 33.0 22.0 36.0 2.0 39.0 70-71 26.729265773965935 33.0 22.0 36.0 2.0 39.0 72-73 26.69484005522817 33.0 22.5 35.5 2.0 39.0 74-75 26.42361158937141 33.0 22.0 35.0 2.0 37.5 76-77 26.177797620209475 33.0 21.0 35.0 2.0 37.0 78-79 25.877269240273844 33.0 20.0 35.0 2.0 37.0 80-81 25.912748809423405 33.0 21.0 35.0 2.0 36.0 82-83 25.757057740985186 33.0 20.0 35.0 2.0 36.0 84-85 25.61018583343676 33.0 20.0 35.0 2.0 36.0 86-87 25.449393267216173 33.0 20.0 35.0 2.0 35.0 88-89 25.26550382814891 32.0 19.0 35.0 2.0 35.0 90-91 25.122547169151183 32.0 18.5 35.0 2.0 35.0 92-93 25.050327571989786 32.0 18.0 35.0 2.0 35.0 94-95 24.98901771124241 32.0 18.0 35.0 2.0 35.0 96-97 24.868332596147507 32.0 16.5 35.0 2.0 35.0 98-99 24.769052632121685 32.0 14.0 35.0 2.0 35.0 100-101 24.729379942628405 32.0 14.0 35.0 2.0 35.0 102-103 23.538466921103193 30.0 7.5 34.0 2.0 35.0 104-105 24.340696229966625 32.0 7.5 34.5 2.0 35.0 106-107 24.45650910531912 32.0 7.0 35.0 2.0 35.0 108-109 24.387238005937505 32.0 6.0 35.0 2.0 35.0 110-111 24.257334139634168 32.0 2.0 35.0 2.0 35.0 112-113 24.23974958761174 32.0 2.0 35.0 2.0 35.0 114-115 24.181833482427784 32.0 2.0 35.0 2.0 35.0 116-117 23.991041626999195 32.0 2.0 35.0 2.0 35.0 118-119 23.82739300278682 32.0 2.0 35.0 2.0 35.0 120-121 23.6847278421644 32.0 2.0 35.0 2.0 35.0 122-123 23.395450415629174 32.0 2.0 35.0 2.0 35.0 124-125 22.354842463818663 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.153655364963079 1101 2 1.745379636131286 1101 3 2.0447230630123805 1101 4 2.4525980398051246 1101 5 1.8785458418221488 1101 6 1.9054386818241618 1101 7 1.6788471972632912 1101 8 2.1149656540010753 1101 9 2.729259509479501 1101 10-11 2.57460454692702 1101 12-13 2.5142331451547832 1101 14-15 2.850186166409163 1101 16-17 2.4161595679069094 1101 18-19 2.465364957566919 1101 20-21 2.867147796334507 1101 22-23 2.720371077159985 1101 24-25 2.5870348150363434 1101 26-27 2.295207593202907 1101 28-29 2.2436289929823907 1101 30-31 2.4496583601522346 1101 32-33 2.6427873303882556 1101 34-35 2.82203974325245 1101 36-37 2.8766743602387344 1101 38-39 2.8940888764467303 1101 40-41 2.6030917738134747 1101 42-43 2.5740739858569626 1101 44-45 2.8574932530077426 1101 46-47 2.7605304197977816 1101 48-49 2.6812537408433386 1101 50-51 2.564015420167891 1101 52-53 2.60071260822831 1101 54-55 2.545661119694241 1101 56-57 2.7626417826521 1101 58-59 2.7349893215776397 1101 60-61 2.8655238649606787 1101 62-63 2.630220579438255 1101 64-65 2.4514261970810978 1101 66-67 2.604162868920767 1101 68-69 2.5558162591732554 1101 70-71 2.5335262500098246 1101 72-73 2.3109344120867377 1101 74-75 2.2412578039785345 1101 76-77 2.521550281640703 1101 78-79 2.451538615974858 1101 80-81 2.326378159208005 1101 82-83 2.336345169815832 1101 84-85 2.2203470146193567 1101 86-87 2.183600325935636 1101 88-89 2.3781900762443335 1101 90-91 2.309646392557891 1101 92-93 2.301952947654943 1101 94-95 2.285545162294312 1101 96-97 2.2914578767508686 1101 98-99 2.1063946544062304 1101 100-101 2.122545092219127 1101 102-103 2.1239330300338324 1101 104-105 2.2121580015094864 1101 106-107 2.34175619522437 1101 108-109 2.369525379930007 1101 110-111 2.3846908523378367 1101 112-113 2.3396207422267175 1101 114-115 2.392683659763719 1101 116-117 2.4132786736387892 1101 118-119 2.361597278986217 1101 120-121 2.438708393557345 1101 122-123 2.456869886244977 1101 124-125 2.395482841796415 1102 1 1.01670217023241 1102 2 0.9499746462484389 1102 3 0.9526332516634106 1102 4 1.639204260569354 1102 5 1.420179446659688 1102 6 1.3125614615412573 1102 7 1.3547447733522269 1102 8 1.5866786305937701 1102 9 1.8979874585245646 1102 10-11 1.575345634252301 1102 12-13 1.918337718668873 1102 14-15 2.15802164467247 1102 16-17 1.625690078250706 1102 18-19 1.8313439849182842 1102 20-21 1.8373934936236722 1102 22-23 1.8336806830816705 1102 24-25 1.577975348137894 1102 26-27 1.6582860583863415 1102 28-29 1.338709731604684 1102 30-31 1.4077100253936194 1102 32-33 1.4812879472308644 1102 34-35 1.8638311219424075 1102 36-37 2.012404793009903 1102 38-39 2.123830328269918 1102 40-41 1.7206148353154873 1102 42-43 1.7856858142812584 1102 44-45 2.1355195202220933 1102 46-47 2.0269276267157608 1102 48-49 2.1386118670437284 1102 50-51 1.9885992850606726 1102 52-53 2.157951746891726 1102 54-55 1.9418821993557245 1102 56-57 2.051777817475667 1102 58-59 2.1126763253581338 1102 60-61 2.4643664672887944 1102 62-63 2.355767852012587 1102 64-65 2.2855885294938396 1102 66-67 2.2468898506444717 1102 68-69 2.0463256449942833 1102 70-71 2.3318720487041737 1102 72-73 2.2140814192318317 1102 74-75 2.3317882185248067 1102 76-77 2.2653883978407663 1102 78-79 2.2504850184272733 1102 80-81 1.8295075840022754 1102 82-83 1.729101007623612 1102 84-85 1.7398829340185884 1102 86-87 1.857549941109962 1102 88-89 1.6185459523711216 1102 90-91 1.6098753720104888 1102 92-93 1.5711004781816413 1102 94-95 1.7364556476840214 1102 96-97 1.83029396017416 1102 98-99 1.9258751981623305 1102 100-101 1.7611051152105333 1102 102-103 1.5103068249710496 1102 104-105 1.400194876547456 1102 106-107 1.6488758206326821 1102 108-109 1.8746879319761014 1102 110-111 1.6650704091457698 1102 112-113 1.6306074216975404 1102 114-115 1.6523255055250274 1102 116-117 1.819702832372954 1102 118-119 1.8851227564551039 1102 120-121 1.8426886639400202 1102 122-123 1.972419927601468 1102 124-125 1.937751981441167 1103 1 1.3994042494001029 1103 2 1.1054541981348223 1103 3 1.3521159732557848 1103 4 1.8391671378866192 1103 5 1.367822305034867 1103 6 1.2388727578427314 1103 7 1.2467084859298545 1103 8 1.5264935407051219 1103 9 2.238043368616278 1103 10-11 1.8555405937772775 1103 12-13 2.0010036270174894 1103 14-15 2.132559024060928 1103 16-17 2.0102055171818094 1103 18-19 1.7998022275519006 1103 20-21 2.1089263748614258 1103 22-23 1.9406239633109301 1103 24-25 2.078424419565149 1103 26-27 1.7763177471174068 1103 28-29 1.6730686187094896 1103 30-31 1.8515195205905712 1103 32-33 1.8165038685783692 1103 34-35 1.8732075739063099 1103 36-37 2.0450557689973543 1103 38-39 2.1109923646156474 1103 40-41 2.1325278552040814 1103 42-43 2.092310725862042 1103 44-45 2.0666320763151234 1103 46-47 2.169683855027401 1103 48-49 2.197125454600016 1103 50-51 1.9157754089885586 1103 52-53 1.9219668089443154 1103 54-55 1.9449524950819175 1103 56-57 2.077786659099239 1103 58-59 2.314950625098813 1103 60-61 2.1511096241327223 1103 62-63 2.0317386785812452 1103 64-65 1.9754061167217358 1103 66-67 1.9898129228082304 1103 68-69 1.882665657832547 1103 70-71 2.081684442325976 1103 72-73 2.0307803203647268 1103 74-75 2.0585567960776032 1103 76-77 2.0488350128648634 1103 78-79 2.145008113911693 1103 80-81 1.7111412259575687 1103 82-83 1.8327687762707683 1103 84-85 1.844815759215372 1103 86-87 1.8891824527271623 1103 88-89 1.838728912464834 1103 90-91 1.8463134565514459 1103 92-93 1.751990417161231 1103 94-95 1.5664240934037004 1103 96-97 1.792567795881304 1103 98-99 1.6749551029521719 1103 100-101 1.5040830385176918 1103 102-103 1.6180966986884897 1103 104-105 1.7326041386567965 1103 106-107 1.5784239590550726 1103 108-109 1.8347483277253183 1103 110-111 1.9788381651212426 1103 112-113 1.808532424359786 1103 114-115 1.6882356924294086 1103 116-117 1.8188267906027953 1103 118-119 1.8768991898576672 1103 120-121 1.902729427501253 1103 122-123 1.8924169173326426 1103 124-125 1.793106601222206 1104 1 1.5134315846138797 1104 2 1.3343500710179654 1104 3 1.2880862303018077 1104 4 1.6083521795149416 1104 5 1.1856821884274709 1104 6 1.142104002044043 1104 7 1.090572873132686 1104 8 1.553151744558221 1104 9 2.0499505336588264 1104 10-11 1.9149641051584894 1104 12-13 1.3511468434980074 1104 14-15 1.7191121674354015 1104 16-17 1.4861429539146727 1104 18-19 0.9109872638359988 1104 20-21 -3.880105245675079 1104 22-23 -0.33393318163110663 1104 24-25 1.1014143587360188 1104 26-27 1.2368846764106287 1104 28-29 0.8824532111166015 1104 30-31 1.5571948001826499 1104 32-33 1.4716902006644723 1104 34-35 1.6595775129828105 1104 36-37 1.7607671464993508 1104 38-39 1.7765046330895444 1104 40-41 1.965438104712483 1104 42-43 1.9281156931068253 1104 44-45 2.0019498556657176 1104 46-47 1.9555987681608542 1104 48-49 1.8748462450869923 1104 50-51 1.7970099423847117 1104 52-53 1.8818381372613224 1104 54-55 1.779776201595272 1104 56-57 2.0671761431702613 1104 58-59 2.1370942108988196 1104 60-61 2.1992858618462137 1104 62-63 1.8888250275357414 1104 64-65 2.022274641655944 1104 66-67 1.9517200281547389 1104 68-69 1.8716081038589394 1104 70-71 2.057428890295963 1104 72-73 1.9199205990610793 1104 74-75 1.935357988997744 1104 76-77 1.8663111075864443 1104 78-79 1.7671298054790938 1104 80-81 1.6054826783121428 1104 82-83 1.639344374452076 1104 84-85 1.477076680705018 1104 86-87 1.4473933670163284 1104 88-89 1.5281278114538495 1104 90-91 1.5225603802866878 1104 92-93 1.3552862240489851 1104 94-95 1.4412890026910183 1104 96-97 1.5113963867813425 1104 98-99 1.474822799475934 1104 100-101 1.0469519857587315 1104 102-103 1.39960095584037 1104 104-105 1.515530270153981 1104 106-107 1.6454569812545508 1104 108-109 1.6691992182679698 1104 110-111 1.6648427473891196 1104 112-113 1.6909237532657961 1104 114-115 1.5945126000632044 1104 116-117 1.7062095042334526 1104 118-119 1.6443013382441656 1104 120-121 1.6384930737170613 1104 122-123 1.4520876555912743 1104 124-125 1.6775420077496648 1105 1 1.057944098010104 1105 2 0.8508207846822344 1105 3 0.7825455553485057 1105 4 1.356714240070918 1105 5 0.9436170189051154 1105 6 0.9126138635571905 1105 7 0.8191946292466845 1105 8 1.1005512936950446 1105 9 1.610221788009941 1105 10-11 1.379930040028853 1105 12-13 1.4755399133885945 1105 14-15 1.7978741484099636 1105 16-17 1.4617257116215399 1105 18-19 1.1121347929881935 1105 20-21 1.338043124497709 1105 22-23 1.3230939346267583 1105 24-25 1.292428286503874 1105 26-27 1.2389736036601455 1105 28-29 0.8206341185781589 1105 30-31 1.2837391855742482 1105 32-33 1.1380015702137705 1105 34-35 1.0414123065362197 1105 36-37 1.2604389467944053 1105 38-39 1.1484592757600183 1105 40-41 1.2207527759162566 1105 42-43 1.48735343137664 1105 44-45 1.7045661117792044 1105 46-47 1.9865711388838179 1105 48-49 1.5805034256954578 1105 50-51 1.6792986978498448 1105 52-53 1.6058518131648647 1105 54-55 1.6414629832282337 1105 56-57 1.9417967746755522 1105 58-59 1.6474003670948 1105 60-61 2.0654638271519303 1105 62-63 1.8073753609132268 1105 64-65 1.6424285338093014 1105 66-67 1.7573633182708122 1105 68-69 1.284647701619285 1105 70-71 1.3591580866493977 1105 72-73 1.3888583253551587 1105 74-75 1.6513249230544957 1105 76-77 1.549790321303 1105 78-79 1.306219956700783 1105 80-81 1.298061750733158 1105 82-83 1.3891390761469538 1105 84-85 1.4114024383571646 1105 86-87 1.4216152586815909 1105 88-89 1.5739795790529953 1105 90-91 1.5642370692193097 1105 92-93 1.5296913263236824 1105 94-95 1.4235154322192614 1105 96-97 1.570593422282883 1105 98-99 1.3275659337941619 1105 100-101 1.4297471323799016 1105 102-103 1.4224240205384575 1105 104-105 1.4952783344640146 1105 106-107 1.6153855381241655 1105 108-109 1.6482996638627299 1105 110-111 1.5688287125534401 1105 112-113 1.5511328289690915 1105 114-115 1.4396796660909708 1105 116-117 1.7363982687738577 1105 118-119 1.637833790161931 1105 120-121 1.6630237285948617 1105 122-123 1.769021502744419 1105 124-125 1.5033153801742714 1106 1 -0.2868176146781991 1106 2 -0.0518747282383174 1106 3 -0.03739944757661462 1106 4 -0.32226514482937674 1106 5 -0.965587388223696 1106 6 -0.3084632531939455 1106 7 -0.32439439406704906 1106 8 0.14271825207349664 1106 9 0.15476205584057823 1106 10-11 -2.4940764792693386 1106 12-13 -0.49801095130275286 1106 14-15 -0.18947060209843158 1106 16-17 -0.39654237670990966 1106 18-19 -3.0585946305268976 1106 20-21 -5.433298053441682 1106 22-23 -6.284770398985881 1106 24-25 -6.176444185427371 1106 26-27 -5.170576736030025 1106 28-29 -1.8887217065740387 1106 30-31 -3.659812183310308 1106 32-33 -4.200145807861507 1106 34-35 -3.667602187818016 1106 36-37 -5.891057306569884 1106 38-39 -6.132332323580847 1106 40-41 -2.529800035921241 1106 42-43 -1.2649168385908105 1106 44-45 -0.4594565417016092 1106 46-47 -0.25844215020856254 1106 48-49 -0.17164599796369018 1106 50-51 -0.2813472216665147 1106 52-53 -0.5179958452578433 1106 54-55 -0.48376660474987787 1106 56-57 -0.31043267965134547 1106 58-59 -0.1590610689274854 1106 60-61 0.07216559526947464 1106 62-63 -0.3089884296913361 1106 64-65 -0.3580668560240845 1106 66-67 -0.7668955777381363 1106 68-69 -0.6291222550281006 1106 70-71 -0.4734477679140703 1106 72-73 -0.3764731496660687 1106 74-75 -0.4216040233177516 1106 76-77 -0.5195068333666732 1106 78-79 -0.5526681692290758 1106 80-81 -0.8505674039589195 1106 82-83 -0.9917230702275361 1106 84-85 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-5.230126209091388 2216 90-91 -5.210307076219102 2216 92-93 -4.902905051409917 2216 94-95 -7.979123913119292 2216 96-97 -10.123475854480134 2216 98-99 -6.888516397990173 2216 100-101 -5.920152783944658 2216 102-103 -6.814157032445877 2216 104-105 -9.648221073026727 2216 106-107 -6.394938314654027 2216 108-109 -8.702920002617063 2216 110-111 -6.087604462480002 2216 112-113 -5.546389677413167 2216 114-115 -5.830763875717775 2216 116-117 -5.425239074233726 2216 118-119 -5.671474193586867 2216 120-121 -6.366280191133068 2216 122-123 -9.188631719154408 2216 124-125 -7.625050832718994 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 217314.0 3 35208.0 4 21035.0 5 18181.0 6 18672.0 7 20910.0 8 23299.0 9 24969.0 10 26367.0 11 27247.0 12 27111.0 13 26991.0 14 27196.0 15 22616.0 16 18164.0 17 16420.0 18 15307.0 19 14912.0 20 15035.0 21 16721.0 22 21018.0 23 28181.0 24 37420.0 25 47492.0 26 56596.0 27 65696.0 28 75813.0 29 86093.0 30 97546.0 31 111926.0 32 132985.0 33 162380.0 34 202094.0 35 257540.0 36 334148.0 37 301827.0 38 62728.0 39 123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.646502852264 17.89936672882147 13.655439724955782 23.798690693958747 2 16.32976054429588 19.3072812246952 38.71631583484563 25.646642396163287 3 19.12262480099597 24.03416034760169 28.115856913844933 28.727357937557407 4 11.877669080123068 15.567641260892795 36.042668435306474 36.512021223677664 5 14.884786089212248 37.246325812079824 32.49918474205607 15.36970335665186 6 33.950048468027305 34.6490045639498 16.71800741899652 14.682939549026367 7 30.930908590030526 31.712095001931694 19.19304043968299 18.163955968354788 8 27.51010871634863 33.68096489764591 19.139697506400008 19.669228879605456 9 27.08518101647372 16.071307555636746 19.202154459926515 37.64135696796301 10-11 25.923390381846588 25.754885561749997 26.00137730208377 22.32034675431964 12-13 26.551635807760704 22.937955192879596 26.560272606082997 23.950136393276704 14-15 23.79853626914379 24.352223589124044 24.036991064390897 27.81224907734127 16-17 24.10020206395874 27.69582071469604 24.278967243389733 23.925009977955487 18-19 24.107511537695085 26.148419200404305 26.12788359497966 23.616185666920952 20-21 24.8273816010277 25.293917316123398 26.160554148990876 23.718146933858026 22-23 24.6024788508693 24.930172047676386 26.1439978901516 24.323351211302718 24-25 24.301496283922845 25.321739258552594 26.242770201766735 24.133994255757823 26-27 24.117715954258976 25.782545987053208 25.951912849267362 24.14782520942046 28-29 24.032644691029873 25.632781846169316 26.226463981031046 24.108109481769766 30-31 24.196972922327646 25.19049979633371 26.199402922629645 24.413124358709 32-33 24.111550706339745 25.254702915962586 26.003398003185126 24.630348374512543 34-35 24.60275874897668 25.242580762795292 25.998561597602787 24.156098890625245 36-37 24.742400551558838 25.145867903205403 25.75758775506972 24.35414379016604 38-39 24.35952871950678 25.190169980821864 25.94453331054694 24.50576798912441 40-41 24.309802093255396 25.110536108646365 26.153884978344077 24.425776819754166 42-43 24.281769151364635 25.270012808860677 25.76023028324065 24.68798775653404 44-45 24.269092581022615 25.223682456728667 25.83649671046264 24.670728251786077 46-47 24.672291704992833 25.70086014507686 25.355339965302505 24.271508184627805 48-49 24.836590594638412 25.42608543200064 25.232423210516693 24.504900762844255 50-51 24.475107597279543 25.68870546246112 25.1519069166128 24.68428002364654 52-53 24.741970718835514 25.528393027725887 25.11066948292116 24.618966770517435 54-55 24.57501592533518 25.421402140357678 25.03302469766725 24.97055723663989 56-57 24.38547654756047 25.82197345818515 25.123475569331948 24.669074424922435 58-59 24.68396509619174 25.29476332374424 25.082655282534468 24.93861629752955 60-61 24.45401953212279 25.391114926901437 25.47341516324753 24.681450377728247 62-63 24.299482096387198 25.5334234104661 25.398657608862795 24.768436884283908 64-65 24.374362475210308 25.415138095765133 25.279138945828876 24.93136048319568 66-67 24.230007449238826 25.6902055861004 25.13445001321342 24.945336951447352 68-69 24.19595015668116 25.26920136070257 25.571735219891362 24.96311326272491 70-71 24.161865550074822 25.312973382484394 25.698382456769693 24.826778610671095 72-73 24.442810877859326 25.57019893317708 25.133021404776006 24.853968784187582 74-75 24.152495010127062 25.771701642887695 25.027644789180513 25.048158557804722 76-77 24.459662431281494 25.72746036738756 25.016832028145213 24.796045173185732 78-79 24.16117197765704 25.66090066606719 25.440020246143895 24.737907110131875 80-81 24.21023284112877 25.8056960097584 25.19830038240291 24.785770766709916 82-83 24.23771889392011 25.83700055595322 25.146230060771817 24.779050489354848 84-85 24.122023261466843 25.71589042508526 25.02900317469671 25.13308313875119 86-87 24.300610381082127 25.671877721956005 25.145708088829178 24.881803808132695 88-89 24.29865030006018 25.65275151540152 25.066276948144505 24.982321236393798 90-91 24.109328504636842 25.702643659766945 25.355352444693334 24.83267539090288 92-93 24.140669756543737 25.438322369481114 25.314556643030073 25.106451230945076 94-95 24.177881998333813 25.386511843404975 25.413084647732635 25.022521510528577 96-97 24.34683186222687 25.32981812148547 25.197067743204897 25.126282273082758 98-99 23.93904634649873 25.30271594043998 25.201618806350375 25.556618906710916 100-101 23.956019591861992 25.311311210297255 25.35900551301944 25.373663684821317 102-103 24.01517276150852 24.813070421788257 25.670152090379545 25.501604726323677 104-105 23.949463304968884 25.06937037408071 25.505720523760132 25.47544579719027 106-107 23.875968392707954 25.356637183296844 25.276718486759737 25.490675937235462 108-109 23.904504328985595 25.29395614058187 25.23902552386186 25.56251400657068 110-111 23.74307375290723 25.33149308618286 25.636496557362857 25.288936603547047 112-113 23.81450203699124 25.33170739881845 25.55206302104501 25.301727543145304 114-115 23.58627131031935 25.71449038184785 25.284043982690807 25.415194325141993 116-117 23.301824058658042 25.617277869005367 25.734215330298603 25.346682742037984 118-119 23.607281542402646 25.442730676000902 25.42280556275278 25.52718221884367 120-121 23.32986995193131 25.72308368872587 25.632165322995 25.314881036347824 122-123 23.169663623610248 25.709069467059376 25.565356962782438 25.555909946547935 124-125 23.50092068205276 25.91409522398719 25.069083397174907 25.51590069678515 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 694.0 1 658.5 2 785.0 3 931.0 4 798.0 5 636.0 6 586.0 7 657.0 8 848.5 9 957.5 10 898.5 11 854.0 12 930.5 13 1024.0 14 1127.0 15 1279.5 16 1422.0 17 1580.0 18 1719.5 19 1960.5 20 2257.5 21 2637.0 22 3250.5 23 3875.5 24 4713.5 25 6222.5 26 8514.0 27 10967.5 28 12637.0 29 14895.0 30 19000.0 31 23634.5 32 29035.5 33 33153.0 34 36113.0 35 39025.5 36 42424.5 37 46250.0 38 48014.5 39 49370.0 40 51469.0 41 55562.0 42 59747.5 43 64811.5 44 70834.5 45 75864.5 46 81115.0 47 85917.5 48 91734.0 49 98861.5 50 103331.0 51 106062.5 52 112384.5 53 120889.0 54 126909.5 55 124750.0 56 117319.0 57 110224.0 58 104839.5 59 95843.5 60 82233.5 61 69780.5 62 57989.5 63 45661.5 64 34273.5 65 26125.0 66 19771.0 67 15361.5 68 11821.5 69 9053.0 70 7182.0 71 5338.5 72 4162.0 73 3398.5 74 2618.0 75 1937.5 76 1353.5 77 827.5 78 543.5 79 373.0 80 260.0 81 179.0 82 129.0 83 103.5 84 81.0 85 54.0 86 42.5 87 36.5 88 29.5 89 20.5 90 18.5 91 17.0 92 9.0 93 2.5 94 4.5 95 4.0 96 5.0 97 6.0 98 3.5 99 4.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09940039355042811 2 0.032225025697156205 3 0.04198460490829494 4 0.04901886766047419 5 0.05214929872819793 6 0.003277745470910745 7 0.0019150872414309974 8 0.0018414300398374975 9 0.0062240335346507416 10-11 0.07385975889788203 12-13 0.005690018823097867 14-15 0.011085408839821736 16-17 0.020126830335423847 18-19 0.017825042785626976 20-21 0.13226991976152744 22-23 0.014510468713919481 24-25 0.014307911409537357 26-27 0.0062240335346507416 28-29 0.003959074585650619 30-31 0.0020255730438212475 32-33 0.005782090325089743 34-35 0.0170700564692936 36-37 0.0024859305537806215 38-39 0.00773400616731749 40-41 0.03301684061428633 42-43 0.013387196389618607 44-45 0.11496047738705498 46-47 0.0036644457792766203 48-49 0.009004592894805362 50-51 0.010901265835837984 52-53 0.012061366760935608 54-55 0.00985165071313061 56-57 0.0062240335346507416 58-59 0.014399982911529231 60-61 0.03054932436090408 62-63 0.007384134459748365 64-65 0.007015848451780864 66-67 0.008783621290024864 68-69 0.00784449196970774 70-71 0.004198460490829494 72-73 8.286435179268738E-4 74-75 0.0011601009250976234 76-77 0.008065463574488238 78-79 0.025411734549757467 80-81 0.0030199452653334956 82-83 0.004585160799195369 84-85 0.002246544648601747 86-87 0.003388231273300995 88-89 0.005855747526683242 90-91 0.004585160799195369 92-93 0.0025964163561708713 94-95 0.003646031478878245 96-97 0.0012705867274878733 98-99 0.0025964163561708713 100-101 0.0026516592573659964 102-103 7.549863163333739E-4 104-105 0.005340147115528743 106-107 7.91814917130124E-4 108-109 0.001381072529878123 110-111 0.002430687652585497 112-113 0.004585160799195369 114-115 0.002817387960951371 116-117 0.005690018823097867 118-119 0.0042168747912278695 120-121 0.0076603489657239895 122-123 0.005082346909951493 124-125 0.05642141642062092 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2715281.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.23789077253542 #Duplication Level Percentage of deduplicated Percentage of total 1 77.04703295997801 37.165863602713564 2 10.594805652783554 10.22142155670428 3 4.297518542612535 6.219096901544672 4 2.1911332317646526 4.227825820077435 5 1.3371658316230024 3.2251029665298443 6 0.8996878654059296 2.603942698849607 7 0.6197304657634354 2.0926143361136367 8 0.45620660681967895 1.760515557558135 9 0.34132130020690504 1.481815763794841 >10 1.9741378145670923 17.64745506988696 >50 0.15679380549563318 5.239574244849779 >100 0.08133269075424607 6.830435077998752 >500 0.0023086974292358124 0.7134229992075025 >1k 8.245347961551402E-4 0.5709134041712305 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 3.682860079674995E-5 0.0 7 0.0 0.0 0.0 3.682860079674995E-5 0.0 8 0.0 0.0 0.0 3.682860079674995E-5 0.0 9 0.0 0.0 0.0 3.682860079674995E-5 0.0 10-11 0.0 0.0 0.0 7.36572015934999E-5 0.0 12-13 0.0 0.0 0.0 7.36572015934999E-5 3.682860079674995E-5 14-15 3.682860079674995E-5 0.0 0.0 7.36572015934999E-5 3.682860079674995E-5 16-17 3.682860079674995E-5 0.0 1.8414300398374975E-5 1.1048580239024985E-4 3.682860079674995E-5 18-19 3.682860079674995E-5 0.0 3.682860079674995E-5 1.2890010278862482E-4 3.682860079674995E-5 20-21 3.682860079674995E-5 0.0 3.682860079674995E-5 1.6572870358537477E-4 3.682860079674995E-5 22-23 3.682860079674995E-5 0.0 3.682860079674995E-5 2.209716047804997E-4 3.682860079674995E-5 24-25 3.682860079674995E-5 0.0 3.682860079674995E-5 4.6035750995937437E-4 3.682860079674995E-5 26-27 3.682860079674995E-5 0.0 3.682860079674995E-5 0.0011048580239024985 3.682860079674995E-5 28-29 7.36572015934999E-5 0.0 3.682860079674995E-5 0.0020992302454147472 3.682860079674995E-5 30-31 7.36572015934999E-5 0.0 3.682860079674995E-5 0.004787718103577494 3.682860079674995E-5 32-33 7.36572015934999E-5 0.0 3.682860079674995E-5 0.01084602293464286 3.682860079674995E-5 34-35 7.36572015934999E-5 0.0 3.682860079674995E-5 0.018377471797578225 3.682860079674995E-5 36-37 7.36572015934999E-5 0.0 3.682860079674995E-5 0.030494081459708958 3.682860079674995E-5 38-39 7.36572015934999E-5 0.0 3.682860079674995E-5 0.053475128356880924 3.682860079674995E-5 40-41 7.36572015934999E-5 0.0 3.682860079674995E-5 0.08023110683571977 3.682860079674995E-5 42-43 7.36572015934999E-5 0.0 3.682860079674995E-5 0.10641624200220898 3.682860079674995E-5 44-45 7.36572015934999E-5 0.0 3.682860079674995E-5 0.13904638230812943 3.682860079674995E-5 46-47 7.36572015934999E-5 0.0 3.682860079674995E-5 0.18127037312160327 3.682860079674995E-5 48-49 7.36572015934999E-5 0.0 3.682860079674995E-5 0.22563042278128856 3.682860079674995E-5 50-51 7.36572015934999E-5 0.0 3.682860079674995E-5 0.27707997809434826 3.682860079674995E-5 52-53 7.36572015934999E-5 0.0 3.682860079674995E-5 0.3279771043954567 3.682860079674995E-5 54-55 7.36572015934999E-5 0.0 3.682860079674995E-5 0.38054993203281723 3.682860079674995E-5 56-57 7.36572015934999E-5 0.0 3.682860079674995E-5 0.443103310486097 3.682860079674995E-5 58-59 7.36572015934999E-5 0.0 3.682860079674995E-5 0.5177732986015076 3.682860079674995E-5 60-61 7.36572015934999E-5 0.0 3.682860079674995E-5 0.6017240941176991 3.682860079674995E-5 62-63 7.36572015934999E-5 0.0 3.682860079674995E-5 0.6886764205988256 3.682860079674995E-5 64-65 7.36572015934999E-5 0.0 3.682860079674995E-5 0.7753893611747735 5.5242901195124924E-5 66-67 7.36572015934999E-5 0.0 3.682860079674995E-5 0.8776807998877465 7.36572015934999E-5 68-69 9.207150199187487E-5 0.0 3.682860079674995E-5 0.9988468965090538 7.36572015934999E-5 70-71 1.1048580239024985E-4 0.0 3.682860079674995E-5 1.1376538929120044 7.36572015934999E-5 72-73 1.1048580239024985E-4 0.0 3.682860079674995E-5 1.2821877367388494 7.36572015934999E-5 74-75 1.1048580239024985E-4 0.0 3.682860079674995E-5 1.4287839822103128 7.36572015934999E-5 76-77 1.1048580239024985E-4 0.0 3.682860079674995E-5 1.593555142174972 7.36572015934999E-5 78-79 1.1048580239024985E-4 0.0 3.682860079674995E-5 1.782725250167478 7.36572015934999E-5 80-81 1.1048580239024985E-4 0.0 3.682860079674995E-5 1.9928876606141317 7.36572015934999E-5 82-83 1.1048580239024985E-4 3.682860079674995E-5 3.682860079674995E-5 2.2131226933786965 7.36572015934999E-5 84-85 1.1048580239024985E-4 3.682860079674995E-5 3.682860079674995E-5 2.4351439132819035 7.36572015934999E-5 86-87 1.1048580239024985E-4 3.682860079674995E-5 3.682860079674995E-5 2.673314474634485 7.36572015934999E-5 88-89 1.1048580239024985E-4 3.682860079674995E-5 3.682860079674995E-5 2.934171454077865 7.36572015934999E-5 90-91 1.1048580239024985E-4 3.682860079674995E-5 3.682860079674995E-5 3.226829930309239 7.36572015934999E-5 92-93 1.1048580239024985E-4 3.682860079674995E-5 3.682860079674995E-5 3.53843672165054 7.36572015934999E-5 94-95 1.1048580239024985E-4 3.682860079674995E-5 3.682860079674995E-5 3.8686971992953954 7.36572015934999E-5 96-97 1.1048580239024985E-4 3.682860079674995E-5 3.682860079674995E-5 4.201940793604788 7.36572015934999E-5 98-99 1.1048580239024985E-4 3.682860079674995E-5 3.682860079674995E-5 4.553156745102993 7.36572015934999E-5 100-101 1.1048580239024985E-4 3.682860079674995E-5 3.682860079674995E-5 4.9553618944043 7.36572015934999E-5 102-103 1.1048580239024985E-4 7.36572015934999E-5 3.682860079674995E-5 5.386459081030656 7.36572015934999E-5 104-105 1.1048580239024985E-4 7.36572015934999E-5 3.682860079674995E-5 5.819213554692865 7.36572015934999E-5 106-107 1.1048580239024985E-4 9.207150199187487E-5 3.682860079674995E-5 6.245081080006084 7.36572015934999E-5 108-109 1.1048580239024985E-4 1.1048580239024985E-4 3.682860079674995E-5 6.700043199948734 7.36572015934999E-5 110-111 1.1048580239024985E-4 1.1048580239024985E-4 3.682860079674995E-5 7.209051291560615 7.36572015934999E-5 112-113 1.1048580239024985E-4 1.1048580239024985E-4 3.682860079674995E-5 7.756692585408287 7.36572015934999E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7135 0.0 52.307568 1 GGTATCA 4310 0.0 48.19912 1 CAACGCA 9060 0.0 39.399822 5 TCAACGC 8995 0.0 39.287025 4 ATCAACG 9035 0.0 39.180656 3 AACGCAG 9085 0.0 38.747334 6 TATCAAC 10105 0.0 37.150776 2 GTACATG 12545 0.0 36.8673 1 TACATGG 13040 0.0 34.62893 2 ACGCAGA 10495 0.0 33.08883 7 CGCAGAG 10720 0.0 31.841036 8 ACATGGG 13175 0.0 31.655579 3 GCAGAGT 12185 0.0 26.497755 9 GTATAGG 655 0.0 26.354069 1 CATGGGG 7740 0.0 25.484884 4 ACGCATA 290 1.4370016E-10 24.649641 8 AGAGTAC 10450 0.0 24.181118 10-11 GAGTACT 4755 0.0 23.393002 12-13 CATGGGA 5810 0.0 23.385914 4 CAGAGTA 11500 0.0 22.801481 10-11 >>END_MODULE