FastQCFastQC Report
Thu 26 May 2016
SRR1364492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2715281
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65510.24126416381950894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61040.2248017792633617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34130.12569601451930756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59050.062.6347771
GTATCAA97650.050.277231
CAACGCA131050.035.55195
TATCAAC137900.035.443822
TCAACGC131850.035.442054
ATCAACG134800.034.8432963
AACGCAG135150.034.385326
GTACATG127150.031.4811021
TACATGG130000.030.628382
ACGCAGA161650.028.4539037
ACATGGG130150.028.3484553
CGCAGAG166750.027.9602038
GAGTACT102750.025.32225212-13
GTACTTT105900.024.96214514-15
GTGGTAT26950.024.7915121
GCAGAGT181750.024.7691769
TATAACG1951.4434609E-624.4537962
CATGGGG80050.024.0504974
CAGAGTA170950.023.63885510-11
AGAGTAC160800.023.5406510-11
Produced by FastQC (version 0.11.5)