##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364492_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2715281 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.006540391215495 31.0 28.0 33.0 25.0 34.0 2 29.14867116884035 31.0 28.0 33.0 25.0 34.0 3 29.222806405672195 31.0 28.0 34.0 25.0 34.0 4 32.652987664996736 35.0 32.0 37.0 26.0 37.0 5 31.76136723970742 35.0 32.0 37.0 20.0 37.0 6 31.803781266101005 35.0 32.0 37.0 21.0 37.0 7 31.20664417421254 35.0 31.0 37.0 17.0 37.0 8 31.341522295482495 35.0 32.0 37.0 17.0 37.0 9 31.9277433900948 37.0 30.0 39.0 11.0 39.0 10-11 32.10321417930594 37.0 31.0 39.0 15.5 39.0 12-13 31.86184652711819 37.0 30.5 39.0 12.5 39.0 14-15 32.80253664353708 38.0 31.0 40.0 12.0 41.0 16-17 32.83878224758321 38.0 31.0 40.0 12.5 41.0 18-19 32.69788393908402 38.0 31.0 40.0 10.0 41.0 20-21 32.56787676855544 37.5 30.5 40.0 10.0 41.0 22-23 32.527100325896285 37.0 30.5 40.0 10.0 41.0 24-25 32.35994635546008 37.0 30.0 40.0 10.0 41.0 26-27 32.369137301074915 37.0 30.0 40.0 9.5 41.0 28-29 32.22771381672836 37.0 30.0 40.0 8.5 41.0 30-31 31.680931549994273 37.0 30.0 40.0 8.0 41.0 32-33 31.60097721009354 37.0 30.0 40.0 5.0 41.0 34-35 31.420769342104926 36.0 30.0 40.0 2.0 41.0 36-37 31.344196420186346 36.0 30.0 40.0 2.0 41.0 38-39 31.090902193916577 36.0 29.5 40.0 2.0 41.0 40-41 30.734507588717335 36.0 28.5 40.0 2.0 41.0 42-43 30.59732326046549 36.0 28.0 40.0 2.0 41.0 44-45 30.478868853720847 36.0 28.0 40.0 2.0 41.0 46-47 30.251498279551914 35.5 28.0 40.0 2.0 41.0 48-49 29.893833455911192 35.0 27.0 40.0 2.0 41.0 50-51 29.532039961978153 35.0 26.0 39.5 2.0 41.0 52-53 29.37935465979396 35.0 26.0 39.0 2.0 41.0 54-55 28.864239465454958 34.5 25.0 39.0 2.0 41.0 56-57 28.83925162810037 34.0 26.0 39.0 2.0 41.0 58-59 28.568464553024164 34.0 25.0 39.0 2.0 40.0 60-61 28.26406548714479 34.0 24.5 38.0 2.0 40.0 62-63 27.964226906902084 34.0 23.5 38.0 2.0 40.0 64-65 27.443333489241077 33.5 22.0 37.0 2.0 40.0 66-67 27.071852416011453 33.0 22.0 37.0 2.0 39.5 68-69 26.839507034446896 33.0 21.5 36.0 2.0 39.0 70-71 26.905397636561375 33.0 22.5 36.0 2.0 39.0 72-73 26.727807913803396 33.0 22.0 35.5 2.0 39.0 74-75 26.389338341040947 33.0 21.0 35.0 2.0 37.5 76-77 26.30929137720921 33.0 22.0 35.0 2.0 37.0 78-79 25.950235169030385 33.0 20.0 35.0 2.0 36.5 80-81 25.797663298936648 33.0 20.0 35.0 2.0 36.0 82-83 25.693348681038906 33.0 20.0 35.0 2.0 36.0 84-85 25.337398781194285 32.5 18.5 35.0 2.0 35.5 86-87 25.25533692461296 32.0 18.5 35.0 2.0 35.0 88-89 25.23213269639496 32.0 19.0 35.0 2.0 35.0 90-91 25.086382772169806 32.0 18.0 35.0 2.0 35.0 92-93 25.148490524553445 32.0 18.5 35.0 2.0 35.0 94-95 25.156877870098896 33.0 19.0 35.0 2.0 35.0 96-97 25.048879655549463 32.5 18.0 35.0 2.0 35.0 98-99 24.953700924508368 32.0 17.5 35.0 2.0 35.0 100-101 24.86778311342362 32.0 16.5 35.0 2.0 35.0 102-103 23.704594110149188 30.0 13.0 34.0 2.0 35.0 104-105 24.655059826220565 32.0 16.5 35.0 2.0 35.0 106-107 24.790710795678237 32.0 15.0 35.0 2.0 35.0 108-109 24.85113327128942 33.0 15.0 35.0 2.0 35.0 110-111 24.778479464924626 33.0 10.5 35.0 2.0 35.0 112-113 24.71003295791485 33.0 9.0 35.0 2.0 35.0 114-115 24.651567185864003 33.0 7.0 35.0 2.0 35.0 116-117 24.5660487809549 33.0 2.0 35.0 2.0 35.0 118-119 24.438241566894916 33.0 2.0 35.0 2.0 35.0 120-121 24.27805869816052 33.0 2.0 35.0 2.0 35.0 122-123 23.992729297630703 32.5 2.0 35.0 2.0 35.0 124-125 23.186308525710597 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6683080239201544 1101 2 0.7197518646484049 1101 3 0.7204858818007658 1101 4 0.7556439360177052 1101 5 0.8340064008215258 1101 6 0.9412647532478005 1101 7 1.3800013094785832 1101 8 1.1927962822114822 1101 9 1.7706253455805125 1101 10-11 1.469478619621583 1101 12-13 1.6715143999811417 1101 14-15 1.4930436878565203 1101 16-17 1.4636843122560492 1101 18-19 1.569916983922191 1101 20-21 1.7088024997869695 1101 22-23 1.742406380972085 1101 24-25 1.8031242452498546 1101 26-27 1.6137370907033812 1101 28-29 1.6439613077723791 1101 30-31 2.231144869117628 1101 32-33 1.959208503871725 1101 34-35 2.0731199100334514 1101 36-37 1.8982528799384077 1101 38-39 2.0109049057108734 1101 40-41 2.3131110431701813 1101 42-43 2.177094943924814 1101 44-45 2.043987048723242 1101 46-47 1.8435643528372445 1101 48-49 1.9378796922092043 1101 50-51 2.008265294062216 1101 52-53 1.8411127559574645 1101 54-55 2.377132825934524 1101 56-57 1.9150165987584131 1101 58-59 2.108395240369621 1101 60-61 2.1102834819883967 1101 62-63 2.1260959451415964 1101 64-65 2.2214452804929365 1101 66-67 2.3847329841168055 1101 68-69 2.340699827859332 1101 70-71 2.0794755294728944 1101 72-73 2.248233528023576 1101 74-75 2.2905393046567717 1101 76-77 2.0656446543761575 1101 78-79 2.210039237060009 1101 80-81 2.23948348155664 1101 82-83 2.1190325191801094 1101 84-85 2.330537431247997 1101 86-87 2.2108129888739896 1101 88-89 2.141893827111236 1101 90-91 2.114484302967245 1101 92-93 2.054059069352604 1101 94-95 2.0461060750071844 1101 96-97 1.9265129529642238 1101 98-99 1.9100556488142786 1101 100-101 1.986373002452087 1101 102-103 1.8057540917176986 1101 104-105 1.8538435426146656 1101 106-107 1.9336523722805055 1101 108-109 1.9233031945516856 1101 110-111 1.9680479547474725 1101 112-113 1.8966022150486133 1101 114-115 1.8873296153749664 1101 116-117 1.961130371614363 1101 118-119 1.9413364198516199 1101 120-121 1.9031181148353866 1101 122-123 1.9853442274410575 1101 124-125 1.7981003611089967 1102 1 0.33899608380996327 1102 2 -2.045831939494718 1102 3 -0.17706684188645738 1102 4 0.27791510843923106 1102 5 0.8730503181459248 1102 6 0.33598035142156846 1102 7 1.0597844098603737 1102 8 0.9485590875368857 1102 9 1.7569130979836274 1102 10-11 1.3671743386404245 1102 12-13 1.509049272617169 1102 14-15 1.4824661222352162 1102 16-17 1.3879140183679297 1102 18-19 1.5129551323663009 1102 20-21 1.6724292889810073 1102 22-23 1.7148362618560355 1102 24-25 1.8340862393337716 1102 26-27 1.4614222369464756 1102 28-29 1.4231985959079694 1102 30-31 1.8185134395848905 1102 32-33 1.3418040210148057 1102 34-35 1.7426857953661532 1102 36-37 1.0689765718348703 1102 38-39 0.9393075286938668 1102 40-41 1.2431800758020906 1102 42-43 1.105849097098048 1102 44-45 1.167737299899681 1102 46-47 0.8521437806689995 1102 48-49 0.9683973960593271 1102 50-51 1.104313646111116 1102 52-53 0.9694172448893461 1102 54-55 1.2205810345207837 1102 56-57 0.8252365592244075 1102 58-59 0.7721073127271296 1102 60-61 0.7976485476681177 1102 62-63 1.1570813044757777 1102 64-65 1.2685912151730427 1102 66-67 1.3503019513260135 1102 68-69 1.2897281255139283 1102 70-71 0.7484952520806445 1102 72-73 1.0986341836525 1102 74-75 1.2907372083265152 1102 76-77 1.184251805077352 1102 78-79 1.0553800309177532 1102 80-81 1.1171940842399053 1102 82-83 0.8786154260976247 1102 84-85 0.9280749675225479 1102 86-87 0.9318401369582752 1102 88-89 1.0316368327235672 1102 90-91 0.8383024301957072 1102 92-93 0.9631791690754952 1102 94-95 1.0203449519797942 1102 96-97 1.0646419455013643 1102 98-99 0.8963179915077291 1102 100-101 0.7167591599432228 1102 102-103 0.5278950028689984 1102 104-105 0.7467617104986601 1102 106-107 1.0113806741408453 1102 108-109 0.9252053011626522 1102 110-111 0.8841880854994137 1102 112-113 0.874937721350225 1102 114-115 1.0247772188012476 1102 116-117 0.7294554453317268 1102 118-119 0.28449489933459304 1102 120-121 0.6588448208491329 1102 122-123 1.0937129007154134 1102 124-125 1.047612786749088 1103 1 0.513639387776319 1103 2 0.4583397219773495 1103 3 0.33433380764150655 1103 4 0.44715408749406294 1103 5 0.7731897066806077 1103 6 0.7696309924719955 1103 7 0.9894579265742074 1103 8 0.8503319930947626 1103 9 1.5467839598307336 1103 10-11 1.1702252103988116 1103 12-13 1.1782636519455423 1103 14-15 0.9572149045933784 1103 16-17 1.0112981554410538 1103 18-19 1.1001873389111978 1103 20-21 1.2993534962421833 1103 22-23 1.2460600670143052 1103 24-25 1.58274699691016 1103 26-27 1.0350561787388273 1103 28-29 1.2022324007509297 1103 30-31 1.796828676601919 1103 32-33 1.517822827461064 1103 34-35 1.6313435664703029 1103 36-37 1.4702174153526464 1103 38-39 1.4064974724922692 1103 40-41 1.65232382684691 1103 42-43 1.561908720647871 1103 44-45 1.439283031296842 1103 46-47 1.4156097363728328 1103 48-49 1.3542665933348594 1103 50-51 1.3904198871836755 1103 52-53 1.3064277266929842 1103 54-55 1.5533747603114705 1103 56-57 1.308282856088077 1103 58-59 1.4572417902402428 1103 60-61 1.5056078531170023 1103 62-63 1.6914522683507691 1103 64-65 1.8057114290092144 1103 66-67 1.9161020551981345 1103 68-69 2.013574868325719 1103 70-71 1.7308538869415777 1103 72-73 1.753219162173103 1103 74-75 1.7610119340261825 1103 76-77 1.5813648025971503 1103 78-79 1.7865743580483056 1103 80-81 1.7654248076622387 1103 82-83 1.6370618477744188 1103 84-85 1.9081384211337706 1103 86-87 1.7746041883993655 1103 88-89 1.6271663752995345 1103 90-91 1.722217034937298 1103 92-93 1.5762866588741922 1103 94-95 1.4563222750179285 1103 96-97 1.4229566232984894 1103 98-99 1.6515698695049856 1103 100-101 1.579776371422934 1103 102-103 1.561599989385254 1103 104-105 1.5355557765806083 1103 106-107 1.510768358095934 1103 108-109 1.5028390930364708 1103 110-111 1.4956318663494663 1103 112-113 1.5088993243600157 1103 114-115 1.2249891274485094 1103 116-117 1.2372109032287177 1103 118-119 1.34197187761961 1103 120-121 1.3892769728998324 1103 122-123 1.4755319181184596 1103 124-125 1.3317586051227899 1104 1 0.610419855369134 1104 2 0.5534803678327656 1104 3 0.6292200119269218 1104 4 0.47302458791230606 1104 5 0.86821346200826 1104 6 0.996041747719552 1104 7 1.210718505636013 1104 8 1.0487935750272612 1104 9 1.6235708598137748 1104 10-11 1.1812966934479476 1104 12-13 1.2621607480289256 1104 14-15 1.2131940455469206 1104 16-17 1.043408057325216 1104 18-19 1.0529017092314206 1104 20-21 1.393724489607564 1104 22-23 1.3913841587945157 1104 24-25 1.615439276285116 1104 26-27 1.5080500891175603 1104 28-29 1.413582755262965 1104 30-31 1.9210810334725608 1104 32-33 1.752590857469638 1104 34-35 1.7541046594581466 1104 36-37 1.6407009993117434 1104 38-39 1.812946162582108 1104 40-41 1.9491653322172482 1104 42-43 1.9228066616291812 1104 44-45 1.6456247176598886 1104 46-47 1.6616138683620214 1104 48-49 1.4673237257975806 1104 50-51 1.8356506484724662 1104 52-53 1.751776430296868 1104 54-55 2.099917714425061 1104 56-57 2.0595667046654995 1104 58-59 2.1176995951759885 1104 60-61 2.085648613571756 1104 62-63 1.9873336624431452 1104 64-65 2.091187718396732 1104 66-67 2.081721833393811 1104 68-69 2.209311155563043 1104 70-71 2.0237575573780617 1104 72-73 2.1662282478532333 1104 74-75 2.2827233261963435 1104 76-77 2.1644535290458577 1104 78-79 2.132044995073823 1104 80-81 2.009404362735726 1104 82-83 2.004951019180332 1104 84-85 2.228419490547921 1104 86-87 2.068055214651011 1104 88-89 1.9804699853122578 1104 90-91 2.026402204769969 1104 92-93 1.85927558491802 1104 94-95 1.7237739711159747 1104 96-97 1.778787337160196 1104 98-99 1.8728224038150003 1104 100-101 1.8714043659538682 1104 102-103 1.8577656787519707 1104 104-105 1.7316358845701707 1104 106-107 1.8519305046449333 1104 108-109 1.8283137461840155 1104 110-111 1.8405511109705834 1104 112-113 1.8453705846690234 1104 114-115 1.6664709775503965 1104 116-117 1.727125428167362 1104 118-119 1.7416393536318928 1104 120-121 1.6513814106066462 1104 122-123 1.7091777478925358 1104 124-125 1.5733515694500895 1105 1 0.2181816948328823 1105 2 0.33887900173796126 1105 3 0.2059764822508363 1105 4 0.41913854138776685 1105 5 0.48964480360436013 1105 6 0.5771914544840016 1105 7 0.8855600367580188 1105 8 0.7318505506319966 1105 9 1.3044864222750476 1105 10-11 0.9067632910624752 1105 12-13 1.1197970802092883 1105 14-15 0.8908573803459703 1105 16-17 0.8514984703815571 1105 18-19 1.0058924194831107 1105 20-21 1.1959339049821196 1105 22-23 1.1695393427863223 1105 24-25 1.350320846241594 1105 26-27 1.1505588366721398 1105 28-29 1.1752530631798237 1105 30-31 1.5189904520534867 1105 32-33 1.3670887643219949 1105 34-35 1.461539732452902 1105 36-37 1.1984935485468036 1105 38-39 1.35249973620585 1105 40-41 1.4347025084886127 1105 42-43 1.4043602633511512 1105 44-45 1.310879571372947 1105 46-47 1.1945986609920425 1105 48-49 1.235737069009101 1105 50-51 1.3868290969996373 1105 52-53 1.3344327343087663 1105 54-55 1.4749512085263063 1105 56-57 1.330481384525335 1105 58-59 1.2919769241815935 1105 60-61 1.2353980352072256 1105 62-63 0.9947483359015798 1105 64-65 1.5768938522274318 1105 66-67 1.7485418600848561 1105 68-69 1.7470227943794256 1105 70-71 1.552976395061087 1105 72-73 1.7294488808767312 1105 74-75 1.8144475793019659 1105 76-77 1.6358439016203619 1105 78-79 1.7702868632158868 1105 80-81 1.6827497767941964 1105 82-83 1.6059739021237007 1105 84-85 1.8807613414571662 1105 86-87 1.8542628054538746 1105 88-89 1.6806065166161126 1105 90-91 1.7609524309663271 1105 92-93 1.474674476012595 1105 94-95 1.3682396239277637 1105 96-97 1.4809998512702087 1105 98-99 1.515400802105269 1105 100-101 1.5411638576298294 1105 102-103 1.3163049099324802 1105 104-105 0.8615598856755184 1105 106-107 1.1600636881815056 1105 108-109 1.1755389787053048 1105 110-111 1.2951362537407114 1105 112-113 1.3127303634906902 1105 114-115 1.2705781219654035 1105 116-117 1.2704715820701686 1105 118-119 1.3122764445775665 1105 120-121 1.3929300203621011 1105 122-123 1.4770575364443452 1105 124-125 1.284476063724565 1106 1 0.24463903328497594 1106 2 0.0805380054681919 1106 3 0.20908167215960916 1106 4 0.030624062639510896 1106 5 0.1701211621449552 1106 6 0.40026965805161296 1106 7 0.5231847745575458 1106 8 0.17347618157377553 1106 9 0.9082729932890601 1106 10-11 0.5125190166565261 1106 12-13 0.38612153574064934 1106 14-15 0.44303797595020455 1106 16-17 0.4032280931730128 1106 18-19 0.48904036177459886 1106 20-21 0.38912273494176475 1106 22-23 0.39196377582906905 1106 24-25 0.2100184823757587 1106 26-27 0.20532742399480242 1106 28-29 0.22821175695128204 1106 30-31 0.7459832356881719 1106 32-33 0.5575407455211838 1106 34-35 0.47703406988300756 1106 36-37 0.5240342957611297 1106 38-39 0.6533942389793168 1106 40-41 0.8327146267323258 1106 42-43 0.8410906184232161 1106 44-45 0.7940493220213973 1106 46-47 0.6890847480606048 1106 48-49 0.6807910136806967 1106 50-51 1.0538681063480198 1106 52-53 0.6278655777743829 1106 54-55 0.996044491472329 1106 56-57 0.7002806916198168 1106 58-59 0.7212700292994754 1106 60-61 0.9659127684530056 1106 62-63 0.9692743742794825 1106 64-65 1.0749761173514258 1106 66-67 1.1151009255187319 1106 68-69 0.9802527681053554 1106 70-71 0.90641803389709 1106 72-73 0.7978068756603349 1106 74-75 1.146892972376591 1106 76-77 0.8176397657955121 1106 78-79 0.7376641621968574 1106 80-81 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-5.121771023880356 2216 88-89 -4.867033280019417 2216 90-91 -4.24343301572517 2216 92-93 -3.9735176170201427 2216 94-95 -3.716582558957686 2216 96-97 -3.9344765685330962 2216 98-99 -3.8162291473335017 2216 100-101 -4.0736263700483235 2216 102-103 -4.039200293319748 2216 104-105 -3.9542701612358897 2216 106-107 -4.029472378524414 2216 108-109 -4.023800094286557 2216 110-111 -3.7245183871372554 2216 112-113 -3.805150285787125 2216 114-115 -3.6694103687876094 2216 116-117 -3.5494485726462734 2216 118-119 -3.7268893472805082 2216 120-121 -3.686069891444795 2216 122-123 -3.7218955860564407 2216 124-125 -3.6768586801975083 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 135637.0 3 46368.0 4 23418.0 5 19393.0 6 20373.0 7 23998.0 8 26467.0 9 27940.0 10 29685.0 11 31239.0 12 33167.0 13 35251.0 14 35796.0 15 27959.0 16 19819.0 17 17540.0 18 15811.0 19 14799.0 20 15018.0 21 15951.0 22 19667.0 23 26618.0 24 36626.0 25 48179.0 26 58626.0 27 66098.0 28 75308.0 29 85263.0 30 95359.0 31 107746.0 32 127012.0 33 151144.0 34 183867.0 35 235423.0 36 335356.0 37 362250.0 38 84948.0 39 162.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.10001327218298 18.58990429281385 13.992383241656958 24.31769919334621 2 17.221018128918804 19.714025282890315 36.845328742024286 26.219627846166592 3 19.522436167748385 23.791423429103656 28.567430037627783 28.118710365520176 4 13.755942994479648 16.43974506463041 34.02960668316155 35.774705257728385 5 14.624858347994184 37.49405678454642 32.43078708980765 15.450297777651741 6 34.09260488244666 34.99811069069171 16.03994139826567 14.869343028595965 7 29.877602602694807 31.88411670860798 19.176083504353176 19.06219718434404 8 28.046010707547396 33.713932370167214 18.311474944950447 19.928581977334943 9 26.984573603984263 15.421497811828683 18.70590925948364 38.88801932470341 10-11 26.571666643648634 25.662454366750914 25.37738063999337 22.388498349607083 12-13 27.157824645748008 22.790495287105653 26.232315942817337 23.819364124329002 14-15 24.075993263277606 24.03594195340642 24.287961368071723 27.60010341524425 16-17 23.926704723432866 27.256570482453206 24.817445931714015 23.999278862399912 18-19 23.768325067936097 25.285596774202457 27.364606127166503 23.581472030694943 20-21 24.433617297343485 24.455070664444847 27.57682100875758 23.53449102945409 22-23 24.213804512917516 23.962591723420648 27.74667541731302 24.076928346348815 24-25 23.96327188224698 24.48462611787493 27.665529647544947 23.88657235233315 26-27 23.73306402990918 24.91922186337197 27.615018380179677 23.73269572653917 28-29 23.648158910660264 24.765448849544747 27.804872299724586 23.781519940070403 30-31 23.727224297225693 24.345674156382646 27.744063958598385 24.183037587793272 32-33 23.703232024548484 24.413103697059547 27.653819800134755 24.229844478257213 34-35 24.229078702575812 24.473660998478405 27.421115212035467 23.876145086910316 36-37 24.40438539260688 24.38862252215871 27.14567009969463 24.061321985539777 38-39 24.047003874943353 24.410688383879954 27.382313073988527 24.159994667188162 40-41 23.85079362448704 24.307568504722894 27.52915266003924 24.31248521075083 42-43 24.116864876988554 24.547671480639668 26.923193784157696 24.41226985821408 44-45 24.057384045186268 24.542937951005975 26.910289459587418 24.48938854422034 46-47 24.534853230173624 25.048411356210355 26.263556652887814 24.15317876072821 48-49 24.78768760269431 24.892741244501718 25.878809161266826 24.44076199153715 50-51 24.341449458729496 25.12577264814958 25.723737331775805 24.809040561345128 52-53 24.68987078494129 25.13244032520409 25.59375563362814 24.583933256226477 54-55 24.449906110203738 24.98997297913808 25.482352719890493 25.077768190767692 56-57 24.397151967496512 25.37204293630339 25.45523883742291 24.775566258777186 58-59 24.575324722436594 24.94066450961512 25.59247562084286 24.89153514710543 60-61 24.474557145282567 25.055491494250504 25.8747989618754 24.595152398591527 62-63 24.231023491261443 25.05646778491234 26.032339384921915 24.680169338904307 64-65 24.287282934389655 24.930065619740873 25.831874082920315 24.95077736294916 66-67 24.14846520463048 25.20272576607139 25.686456587000905 24.96235244229723 68-69 24.131902468056616 24.829485198970914 26.061405032016584 24.977207300955882 70-71 24.285135533294046 24.962673099587505 25.911001031231585 24.84119033588686 72-73 24.392110525325524 25.21374191678241 25.43419623733143 24.95995132056063 74-75 24.11946821334667 25.397243392535735 25.34418921392528 25.13909918019232 76-77 24.42331429446912 25.321726237005958 25.344523522913576 24.910435945611347 78-79 24.209982795268857 25.334763040414636 25.629635522291476 24.825618642025027 80-81 24.154201692277947 25.33260903591717 25.613759195661583 24.8994300761433 82-83 24.237664974021055 25.390126086629866 25.419036591491988 24.953172347857087 84-85 23.93310305636875 25.278230871869244 25.313531085732933 25.47513498602907 86-87 24.207266197873768 25.180207577768925 25.464065011546726 25.14846121281058 88-89 24.159154757362504 25.22915067669793 25.30785446906429 25.303840096875273 90-91 24.01907725736804 25.313605435913487 25.543803113865078 25.123514192853396 92-93 24.053427057000253 25.05620179020748 25.5758105432496 25.314560609542667 94-95 24.068382458556922 24.95283904875201 25.66800605486949 25.310772437821573 96-97 24.1998114701224 25.040922105929187 25.418276496614727 25.340989927333684 98-99 23.79070527138812 24.91874689949217 25.296019086054077 25.994528743065636 100-101 23.744712492742366 25.021853464761644 25.601046240955466 25.632387801540524 102-103 23.888588936087466 24.506268686179723 25.79261811654357 25.81252426118924 104-105 23.826453610206556 24.636261386512007 25.687518092096784 25.849766911184652 106-107 23.700138420372564 24.91944199482963 25.402596675590853 25.977822909206953 108-109 23.887785463088353 24.865640057143256 25.2549367818653 25.99163769790309 110-111 23.642425971139946 24.948812744784092 25.588379521709676 25.820381762366285 112-113 23.73683377036622 24.892313131610734 25.58185527827701 25.78899781974603 114-115 23.388147875888908 25.2017565795313 25.383708549809096 26.0263869947707 116-117 23.343108871604816 25.036395864737386 25.676753161090875 25.94374210256692 118-119 23.361264473350328 24.89661169196637 25.509465246097136 26.232658588586165 120-121 23.072225891878364 25.097354330354865 25.767155069560904 26.063264708205864 122-123 23.062330298931684 25.102490468197708 25.53033281435152 26.30484641851909 124-125 23.121848000537746 25.245645325993976 25.316712353489862 26.315794319978416 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 213.0 1 188.0 2 265.5 3 389.5 4 319.0 5 261.5 6 489.0 7 1093.5 8 1830.5 9 2213.5 10 1885.0 11 1402.0 12 1347.5 13 1393.0 14 1478.0 15 1692.5 16 1978.0 17 2258.5 18 2537.5 19 2820.0 20 3117.5 21 3585.0 22 4254.5 23 4844.0 24 5565.5 25 7032.5 26 9273.5 27 11684.0 28 13524.5 29 15709.5 30 19347.5 31 23720.5 32 29104.5 33 32929.0 34 35417.5 35 38264.0 36 41660.0 37 45330.5 38 46872.5 39 48544.5 40 50590.5 41 54093.0 42 58081.5 43 62959.0 44 69201.5 45 74414.0 46 79679.0 47 84539.5 48 90664.0 49 98338.0 50 102511.0 51 104888.5 52 111474.5 53 120198.5 54 127480.0 55 126080.0 56 118143.0 57 110566.5 58 105086.5 59 96729.5 60 83188.5 61 70589.0 62 58980.0 63 46667.5 64 35177.5 65 26320.5 66 19380.0 67 15411.5 68 11905.0 69 8767.0 70 6972.5 71 5183.5 72 4046.0 73 3420.0 74 2701.5 75 1919.0 76 1268.0 77 740.5 78 425.5 79 252.0 80 171.0 81 115.5 82 76.5 83 45.5 84 34.0 85 29.0 86 14.0 87 9.0 88 9.0 89 5.5 90 4.5 91 3.0 92 1.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1046300548635666 2 2.209716047804997E-4 3 0.0 4 0.18219108814152202 5 0.0 6 1.1048580239024985E-4 7 0.04312629153299419 8 0.0 9 0.0 10-11 6.81329114739874E-4 12-13 6.445005139431241E-4 14-15 5.156004111544993E-4 16-17 0.0046588180007888684 18-19 0.0016757013362521226 20-21 0.0032961597713091206 22-23 0.0019519158422277474 24-25 0.005174418411943368 26-27 0.0047140609019839935 28-29 0.003498717075691245 30-31 9.023007195203737E-4 32-33 5.5242901195124924E-5 34-35 0.001454729731471623 36-37 0.001454729731471623 38-39 5.708433123496242E-4 40-41 0.0017861871386423726 42-43 9.391293203171238E-4 44-45 0.0011601009250976234 46-47 3.3145740717074954E-4 48-49 5.5242901195124924E-5 50-51 0.04262910542223807 52-53 5.5242901195124924E-5 54-55 0.14206632757346294 56-57 1.1048580239024985E-4 58-59 1.8414300398374975E-5 60-61 0.0 62-63 5.892576127479992E-4 64-65 0.06793035416960529 66-67 0.035410699666075074 68-69 0.0017493585378456225 70-71 0.002983116664536746 72-73 0.07441218790983327 74-75 0.004861375305170994 76-77 0.0016757013362521226 78-79 0.001454729731471623 80-81 2.209716047804997E-4 82-83 1.8414300398374975E-4 84-85 0.0 86-87 3.498717075691245E-4 88-89 0.001362658229479748 90-91 2.209716047804997E-4 92-93 0.002393859051788747 94-95 0.004087974688439244 96-97 0.002264958949000122 98-99 0.0 100-101 0.0013442439290813732 102-103 0.0014363154310732481 104-105 2.5780020557724965E-4 106-107 5.5242901195124924E-5 108-109 0.0 110-111 2.0255730438212475E-4 112-113 3.682860079674995E-5 114-115 1.473144031869998E-4 116-117 0.0 118-119 0.0011785152254959984 120-121 0.0025964163561708713 122-123 0.0010496151227073734 124-125 0.008783621290024864 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2715281.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.683813551893955 #Duplication Level Percentage of deduplicated Percentage of total 1 76.65240583980733 34.25121810850058 2 10.32024166189158 9.222955084609035 3 4.317358082770733 5.787480708218694 4 2.302412873037723 4.115223501531924 5 1.410413000891403 3.151131578149935 6 0.9413100775489982 2.5236794399830966 7 0.6670138159020821 2.086330469041417 8 0.48120563299964464 1.7201682228061783 9 0.35964165509770024 1.4463144595692174 >10 2.2347116928355537 18.737326379598095 >50 0.20002021897612088 6.173515988576901 >100 0.10806949754145637 8.503138097386866 >500 0.0041737964636411355 1.185043487108898 >1k 8.517951966605937E-4 0.6148067270677462 >5k 1.7035903933211875E-4 0.4816677478513173 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6551 0.24126416381950894 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6104 0.2248017792633617 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3413 0.12569601451930756 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.682860079674995E-5 6 0.0 0.0 0.0 0.0 3.682860079674995E-5 7 3.682860079674995E-5 0.0 0.0 0.0 3.682860079674995E-5 8 3.682860079674995E-5 0.0 0.0 0.0 3.682860079674995E-5 9 3.682860079674995E-5 0.0 0.0 3.682860079674995E-5 3.682860079674995E-5 10-11 3.682860079674995E-5 0.0 0.0 9.207150199187487E-5 3.682860079674995E-5 12-13 3.682860079674995E-5 0.0 0.0 1.1048580239024985E-4 3.682860079674995E-5 14-15 3.682860079674995E-5 0.0 0.0 1.1048580239024985E-4 3.682860079674995E-5 16-17 3.682860079674995E-5 0.0 0.0 1.473144031869998E-4 7.36572015934999E-5 18-19 3.682860079674995E-5 0.0 0.0 2.209716047804997E-4 7.36572015934999E-5 20-21 3.682860079674995E-5 0.0 0.0 3.498717075691245E-4 7.36572015934999E-5 22-23 3.682860079674995E-5 0.0 0.0 4.419432095609994E-4 7.36572015934999E-5 24-25 3.682860079674995E-5 0.0 0.0 6.813291147398741E-4 7.36572015934999E-5 26-27 3.682860079674995E-5 0.0 0.0 0.0013258296286829982 7.36572015934999E-5 28-29 3.682860079674995E-5 0.0 0.0 0.0021176445458131223 1.1048580239024985E-4 30-31 3.682860079674995E-5 0.0 0.0 0.004566746498796994 1.1048580239024985E-4 32-33 3.682860079674995E-5 0.0 0.0 0.010459322626276985 1.1048580239024985E-4 34-35 3.682860079674995E-5 0.0 0.0 0.01799077148921235 1.1048580239024985E-4 36-37 3.682860079674995E-5 0.0 0.0 0.03012579545174146 1.1048580239024985E-4 38-39 3.682860079674995E-5 0.0 0.0 0.0520940558270028 1.1048580239024985E-4 40-41 3.682860079674995E-5 0.0 0.0 0.0788868629066384 1.1048580239024985E-4 42-43 5.5242901195124924E-5 0.0 0.0 0.1053666268795016 1.473144031869998E-4 44-45 7.36572015934999E-5 1.8414300398374975E-5 0.0 0.13735226667147893 1.473144031869998E-4 46-47 7.36572015934999E-5 3.682860079674995E-5 0.0 0.17917114287618852 1.473144031869998E-4 48-49 7.36572015934999E-5 3.682860079674995E-5 0.0 0.22262889181635342 1.473144031869998E-4 50-51 7.36572015934999E-5 3.682860079674995E-5 0.0 0.27372857542184403 1.473144031869998E-4 52-53 7.36572015934999E-5 3.682860079674995E-5 0.0 0.32352084369904993 1.473144031869998E-4 54-55 7.36572015934999E-5 3.682860079674995E-5 0.0 0.3753386850200771 1.473144031869998E-4 56-57 7.36572015934999E-5 3.682860079674995E-5 0.0 0.43663989104626744 1.473144031869998E-4 58-59 7.36572015934999E-5 3.682860079674995E-5 0.0 0.5101129496357836 1.473144031869998E-4 60-61 7.36572015934999E-5 3.682860079674995E-5 0.0 0.5914489144954058 1.473144031869998E-4 62-63 7.36572015934999E-5 3.682860079674995E-5 0.0 0.6736871800745485 1.473144031869998E-4 64-65 7.36572015934999E-5 3.682860079674995E-5 0.0 0.7557413026497073 1.473144031869998E-4 66-67 7.36572015934999E-5 3.682860079674995E-5 0.0 0.8538526951722492 1.473144031869998E-4 68-69 7.36572015934999E-5 3.682860079674995E-5 0.0 0.9700469306859953 1.473144031869998E-4 70-71 1.1048580239024985E-4 3.682860079674995E-5 0.0 1.104784366700905 1.473144031869998E-4 72-73 1.1048580239024985E-4 3.682860079674995E-5 0.0 1.2434808773014652 1.473144031869998E-4 74-75 1.2890010278862482E-4 3.682860079674995E-5 0.0 1.3835400461315053 1.473144031869998E-4 76-77 1.473144031869998E-4 3.682860079674995E-5 0.0 1.5408350001344244 1.473144031869998E-4 78-79 1.473144031869998E-4 3.682860079674995E-5 0.0 1.7188828706863122 1.473144031869998E-4 80-81 1.473144031869998E-4 3.682860079674995E-5 0.0 1.9166892855656559 1.6572870358537477E-4 82-83 1.473144031869998E-4 3.682860079674995E-5 0.0 2.1238133364465774 1.8414300398374975E-4 84-85 1.473144031869998E-4 3.682860079674995E-5 0.0 2.335246333620719 1.8414300398374975E-4 86-87 1.473144031869998E-4 3.682860079674995E-5 0.0 2.5626445292402518 1.8414300398374975E-4 88-89 1.473144031869998E-4 3.682860079674995E-5 0.0 2.8144048442868344 1.8414300398374975E-4 90-91 1.473144031869998E-4 3.682860079674995E-5 0.0 3.0992924857500936 1.8414300398374975E-4 92-93 1.473144031869998E-4 3.682860079674995E-5 0.0 3.403146856623679 1.8414300398374975E-4 94-95 1.473144031869998E-4 3.682860079674995E-5 0.0 3.7246237129785094 1.8414300398374975E-4 96-97 1.473144031869998E-4 3.682860079674995E-5 0.0 4.051201330543689 1.8414300398374975E-4 98-99 1.6572870358537477E-4 7.36572015934999E-5 0.0 4.399618308381342 1.8414300398374975E-4 100-101 1.8414300398374975E-4 7.36572015934999E-5 0.0 4.7978091401958025 1.8414300398374975E-4 102-103 1.8414300398374975E-4 7.36572015934999E-5 0.0 5.229992770545664 1.8414300398374975E-4 104-105 2.5780020557724965E-4 7.36572015934999E-5 0.0 5.662250058097118 1.8414300398374975E-4 106-107 2.5780020557724965E-4 7.36572015934999E-5 0.0 6.091450571782442 1.8414300398374975E-4 108-109 2.5780020557724965E-4 7.36572015934999E-5 0.0 6.548401436168117 1.8414300398374975E-4 110-111 2.5780020557724965E-4 7.36572015934999E-5 0.0 7.0567466129656555 1.8414300398374975E-4 112-113 2.5780020557724965E-4 7.36572015934999E-5 0.0 7.600889189737637 1.8414300398374975E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5905 0.0 62.634777 1 GTATCAA 9765 0.0 50.27723 1 CAACGCA 13105 0.0 35.5519 5 TATCAAC 13790 0.0 35.44382 2 TCAACGC 13185 0.0 35.44205 4 ATCAACG 13480 0.0 34.843296 3 AACGCAG 13515 0.0 34.38532 6 GTACATG 12715 0.0 31.481102 1 TACATGG 13000 0.0 30.62838 2 ACGCAGA 16165 0.0 28.453903 7 ACATGGG 13015 0.0 28.348455 3 CGCAGAG 16675 0.0 27.960203 8 GAGTACT 10275 0.0 25.322252 12-13 GTACTTT 10590 0.0 24.962145 14-15 GTGGTAT 2695 0.0 24.791512 1 GCAGAGT 18175 0.0 24.769176 9 TATAACG 195 1.4434609E-6 24.453796 2 CATGGGG 8005 0.0 24.050497 4 CAGAGTA 17095 0.0 23.638855 10-11 AGAGTAC 16080 0.0 23.54065 10-11 >>END_MODULE