FastQCFastQC Report
Thu 26 May 2016
SRR1364491_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364491_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834864
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41610.2267743004386156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29430.1603933588538442No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25970.1415363754479896No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97950.042.727851
GGTATCA59950.039.126161
ATCAACG110800.035.926063
GTACATG108700.035.544741
CAACGCA111900.035.3542755
TATCAAC119700.035.3426172
TCAACGC114100.034.622274
AACGCAG111100.034.4102486
TACATGG109100.033.1045072
ACATGGG107100.030.2226583
ACGCAGA126900.029.7521367
CGCAGAG125250.029.6702428
GAGTACT55050.028.9577312-13
CATGGGA63350.027.6521154
GCAGAGT128250.026.7008259
CGCATAG2905.456968E-1226.6968238
GTACACG2905.456968E-1226.6873551
AACGCAT3800.026.6398726
ACGCATA3150.026.46727
GTACTTT62850.025.54977414-15
Produced by FastQC (version 0.11.5)