FastQCFastQC Report
Thu 26 May 2016
SRR1364491_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364491_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834864
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95800.5221095405436044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91780.5002005598235074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55430.30209323415795397No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24910.13575938053174513No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21710.11831939587893163No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80800.052.7829671
GTATCAA129150.043.3219071
GTACATG100800.035.3275031
TATCAAC163200.034.0285952
ATCAACG161550.033.1589663
TACATGG103250.033.1260872
CAACGCA162000.032.608845
TCAACGC165200.032.1738554
AACGCAG163900.032.0493436
ACATGGG102950.030.1550223
GAGTACT121750.028.20993212-13
CGCAGAG191450.027.5829728
ACGCAGA189650.027.3841197
GTACTTT132600.026.23803714-15
CATGGGA60900.026.1241574
CCACGCA4800.026.0264786
GCAGAGT195850.025.9005479
CATGGGG41950.025.5675334
GTATCAC6450.024.9691181
ACCGCAG5350.024.4628076
Produced by FastQC (version 0.11.5)