##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364491_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1834864 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.774493368445835 31.0 28.0 31.0 24.0 34.0 2 28.891337995622564 31.0 28.0 33.0 24.0 34.0 3 28.9565826132073 31.0 28.0 34.0 24.0 34.0 4 32.338476857140364 35.0 32.0 37.0 26.0 37.0 5 31.44491580847409 35.0 32.0 37.0 18.0 37.0 6 31.50349726192241 35.0 32.0 37.0 18.0 37.0 7 30.913297116298537 35.0 31.0 37.0 13.0 37.0 8 31.029826188752953 35.0 31.0 37.0 17.0 37.0 9 31.523495474323983 37.0 30.0 39.0 10.0 39.0 10-11 31.707055672791007 37.0 30.0 39.0 10.5 39.0 12-13 31.468290837904064 37.0 30.0 39.0 10.0 39.0 14-15 32.33994917334473 37.0 30.5 40.0 10.0 41.0 16-17 32.42825980563137 37.0 31.0 40.0 10.0 41.0 18-19 32.3796564759023 37.0 30.5 40.0 10.0 41.0 20-21 32.315287945046606 37.0 30.5 40.0 10.0 41.0 22-23 32.27375516659545 37.0 30.0 40.0 10.0 41.0 24-25 32.13458763156288 37.0 30.0 40.0 8.5 41.0 26-27 32.134151631946565 37.0 30.0 40.0 8.0 41.0 28-29 31.978502766417563 37.0 30.0 40.0 8.0 41.0 30-31 31.419865178018643 36.5 30.0 40.0 2.0 41.0 32-33 31.263887677778843 36.0 30.0 40.0 2.0 41.0 34-35 31.057746241683308 36.0 30.0 40.0 2.0 41.0 36-37 30.93246774692838 36.0 29.0 40.0 2.0 41.0 38-39 30.684102200490063 36.0 29.0 40.0 2.0 41.0 40-41 30.3283434085578 35.0 27.5 40.0 2.0 41.0 42-43 30.16545177190244 35.0 27.5 40.0 2.0 41.0 44-45 30.003403522004902 35.0 27.0 40.0 2.0 41.0 46-47 29.712305380671268 35.0 26.5 40.0 2.0 41.0 48-49 29.316349059112827 35.0 25.5 40.0 2.0 41.0 50-51 28.97567721640405 35.0 24.0 40.0 2.0 41.0 52-53 28.846994927144465 35.0 24.0 39.5 2.0 41.0 54-55 28.39073222865564 34.5 23.0 39.0 2.0 41.0 56-57 28.393864068399623 34.5 23.0 39.0 2.0 41.0 58-59 28.179384684641477 34.0 23.0 39.0 2.0 41.0 60-61 27.91946242337307 34.0 22.5 39.0 2.0 41.0 62-63 27.659130594965077 34.0 22.0 38.5 2.0 40.0 64-65 27.193632062103784 34.0 20.0 38.0 2.0 40.0 66-67 26.854103083389283 33.0 19.5 37.5 2.0 40.0 68-69 26.662541474463502 33.0 18.5 37.0 2.0 40.0 70-71 26.705666196513747 33.0 20.0 37.0 2.0 39.5 72-73 26.49986402261966 33.0 19.0 36.5 2.0 39.0 74-75 26.142821211817335 33.0 17.5 36.0 2.0 39.0 76-77 26.02936103166229 33.0 18.5 36.0 2.0 39.0 78-79 25.641762277749194 33.0 16.0 35.0 2.0 37.5 80-81 25.428037718326806 33.0 14.0 35.0 2.0 37.0 82-83 25.26500220179806 32.5 13.0 35.0 2.0 37.0 84-85 24.869803702072744 32.0 8.0 35.0 2.0 36.0 86-87 24.742004312036208 32.0 9.0 35.0 2.0 36.0 88-89 24.668253614436818 32.0 7.5 35.0 2.0 36.0 90-91 24.494526842316375 32.0 7.0 35.0 2.0 35.0 92-93 24.49971196775347 32.0 7.0 35.0 2.0 35.0 94-95 24.4712024978418 32.0 7.0 35.0 2.0 35.0 96-97 24.355216244909705 32.0 6.0 35.0 2.0 35.0 98-99 24.245787426207066 32.0 2.0 35.0 2.0 35.0 100-101 24.153045947819564 32.0 2.0 35.0 2.0 35.0 102-103 23.024615448338405 30.0 2.0 34.0 2.0 35.0 104-105 23.921276454276722 31.5 2.0 34.5 2.0 35.0 106-107 24.041670118330295 32.0 2.0 35.0 2.0 35.0 108-109 24.095556400910368 32.0 2.0 35.0 2.0 35.0 110-111 24.023801764054447 32.0 2.0 35.0 2.0 35.0 112-113 23.94685082927127 32.0 2.0 35.0 2.0 35.0 114-115 23.880873732331114 32.0 2.0 35.0 2.0 35.0 116-117 23.79119896624491 32.0 2.0 35.0 2.0 35.0 118-119 23.66689138813558 32.0 2.0 35.0 2.0 35.0 120-121 23.50266777265236 32.0 2.0 35.0 2.0 35.0 122-123 23.219692576670532 32.0 2.0 35.0 2.0 35.0 124-125 22.477510322290918 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8923406703874726 1101 2 0.9303836569036079 1101 3 1.005397497140006 1101 4 1.037700744859798 1101 5 1.2174333207108248 1101 6 1.1115003165213473 1101 7 1.6749683358947678 1101 8 1.4546888820886963 1101 9 2.0778618614744104 1101 10-11 1.7316778930786434 1101 12-13 1.8803080182667031 1101 14-15 1.8863074054492444 1101 16-17 1.7963793340177219 1101 18-19 1.9418238780499593 1101 20-21 2.080193261692486 1101 22-23 2.1365031566054213 1101 24-25 2.273173755548285 1101 26-27 2.0278679947895135 1101 28-29 2.101572998433287 1101 30-31 2.4461827939567264 1101 32-33 2.339458641815721 1101 34-35 2.459789636682217 1101 36-37 2.3531366595310814 1101 38-39 2.523334765597866 1101 40-41 2.626639247211706 1101 42-43 2.481758623737001 1101 44-45 2.3088851483856487 1101 46-47 1.8672256296805863 1101 48-49 1.8163133059743366 1101 50-51 2.0099771873590306 1101 52-53 1.8815765121903496 1101 54-55 2.292664188107082 1101 56-57 1.960996265207001 1101 58-59 2.0516988297597187 1101 60-61 2.1051940542467733 1101 62-63 2.0907764901330346 1101 64-65 2.282149514966143 1101 66-67 2.4364142761895877 1101 68-69 2.3043931679719236 1101 70-71 1.9208514015215847 1101 72-73 2.1083830626393407 1101 74-75 2.1188944040581 1101 76-77 1.8844171161691072 1101 78-79 1.9522252103620765 1101 80-81 1.8956304039689478 1101 82-83 1.8491180838319288 1101 84-85 2.133650921486705 1101 86-87 1.9684074826230358 1101 88-89 1.9081405270356342 1101 90-91 1.8392557946286452 1101 92-93 1.781274912245486 1101 94-95 1.6894066538160324 1101 96-97 1.6346392407012367 1101 98-99 1.7708255934474622 1101 100-101 1.8093937589566202 1101 102-103 1.6752280600556837 1101 104-105 1.6925514382014732 1101 106-107 1.7745811106896312 1101 108-109 1.7325255396095294 1101 110-111 1.73635451325611 1101 112-113 1.6648582189906378 1101 114-115 1.6184115386242084 1101 116-117 1.7186785449412731 1101 118-119 1.6153906398029143 1101 120-121 1.5374803767344538 1101 122-123 1.6646591106004571 1101 124-125 1.510090819060661 1102 1 0.20160085245106174 1102 2 -1.941860635886112 1102 3 -0.23692210474685993 1102 4 0.20385522560999902 1102 5 0.5756687944819134 1102 6 0.03267610224594364 1102 7 0.899218287816467 1102 8 0.43861532281744786 1102 9 1.2362296051776838 1102 10-11 1.1355235905929923 1102 12-13 1.2000034645047677 1102 14-15 1.1397929200381895 1102 16-17 1.1576379856034364 1102 18-19 1.1998883713294504 1102 20-21 1.5056985147708843 1102 22-23 1.4425374587180144 1102 24-25 1.5680895444391467 1102 26-27 1.23162708642894 1102 28-29 1.2046864654697593 1102 30-31 1.594133099940155 1102 32-33 1.220493501742876 1102 34-35 1.4498135512842865 1102 36-37 0.983233830236621 1102 38-39 0.8537424353650351 1102 40-41 1.0823694311889867 1102 42-43 0.9571351544657318 1102 44-45 0.9322543671985315 1102 46-47 0.7452373117931081 1102 48-49 0.6245619843080057 1102 50-51 0.9407748314808977 1102 52-53 0.7833610701597991 1102 54-55 1.0463928980372543 1102 56-57 0.6115935045621015 1102 58-59 0.6405450532135042 1102 60-61 0.6782352958085767 1102 62-63 0.918118954152817 1102 64-65 1.0676244843217582 1102 66-67 1.2055858902876935 1102 68-69 1.0791071566741977 1102 70-71 0.5454563714190783 1102 72-73 0.7783962624796814 1102 74-75 0.9511475250748198 1102 76-77 0.9187111419338834 1102 78-79 0.8758696058841196 1102 80-81 0.9012557129067211 1102 82-83 0.5328684558558692 1102 84-85 0.6053753311093502 1102 86-87 0.6945260247684928 1102 88-89 0.7639271653877557 1102 90-91 0.6109993765442141 1102 92-93 0.5479698297107696 1102 94-95 0.5362221583926505 1102 96-97 0.6652251277922474 1102 98-99 0.40352023206449417 1102 100-101 0.34711920015251607 1102 102-103 0.19494016399406533 1102 104-105 0.5332189999686605 1102 106-107 0.8298788319610892 1102 108-109 0.7133569064251937 1102 110-111 0.6921595447316093 1102 112-113 0.6657887363108088 1102 114-115 0.8468511796064959 1102 116-117 0.5196365575017197 1102 118-119 0.19462433128270717 1102 120-121 0.4637781987076721 1102 122-123 0.8924475547370143 1102 124-125 0.8967960262861148 1103 1 0.6919883026752949 1103 2 0.5283102401237549 1103 3 0.4568148640093028 1103 4 0.3848435927713325 1103 5 0.8107547043929202 1103 6 0.7671363236902273 1103 7 1.0092004816205034 1103 8 0.8388034717694097 1103 9 1.6117188980417723 1103 10-11 1.0867239603785883 1103 12-13 1.0881702781111926 1103 14-15 0.9713844480500029 1103 16-17 1.018609913916663 1103 18-19 1.1689025465620944 1103 20-21 1.306220336710581 1103 22-23 1.309995677038991 1103 24-25 1.613849745603268 1103 26-27 1.0869255239597244 1103 28-29 1.3395650670421766 1103 30-31 1.8869806083291714 1103 32-33 1.7122659373079365 1103 34-35 1.64639499289364 1103 36-37 1.7004035310823866 1103 38-39 1.7548854272707857 1103 40-41 1.7981030628613368 1103 42-43 1.7471792458300541 1103 44-45 1.564418683006096 1103 46-47 1.4642035000767883 1103 48-49 1.5970301580775121 1103 50-51 1.6511402366128536 1103 52-53 1.4961188621254315 1103 54-55 1.7919539430402267 1103 56-57 1.6104516098379733 1103 58-59 1.7724507055039034 1103 60-61 1.7741062592272456 1103 62-63 1.6842959828740796 1103 64-65 1.8982403465242434 1103 66-67 2.105343542923059 1103 68-69 2.154801952836138 1103 70-71 1.8958337134151897 1103 72-73 2.013723493359606 1103 74-75 2.025654474658616 1103 76-77 1.7664704080161187 1103 78-79 1.9186439393374357 1103 80-81 1.9378108551905306 1103 82-83 1.8730840083935867 1103 84-85 1.9929672451403349 1103 86-87 1.884289072470061 1103 88-89 1.8744060403353586 1103 90-91 1.8157713969499056 1103 92-93 1.6804184689283126 1103 94-95 1.6163732815939937 1103 96-97 1.4373484090070967 1103 98-99 1.597604700656376 1103 100-101 1.703878776866926 1103 102-103 1.532505079098751 1103 104-105 1.535400562763492 1103 106-107 1.56733483114375 1103 108-109 1.5235786397527242 1103 110-111 1.5513540388146758 1103 112-113 1.4122922957213646 1103 114-115 1.244575153013173 1103 116-117 1.3352384329980325 1103 118-119 1.431193088056773 1103 120-121 1.3542886748455665 1103 122-123 1.4048262409575791 1103 124-125 1.287201916240214 1104 1 0.5369292960710297 1104 2 0.40527103445554147 1104 3 0.3861308020081893 1104 4 0.2628494891976061 1104 5 0.6010451910620489 1104 6 0.7872357958210863 1104 7 1.0775252730658416 1104 8 0.7543288269556427 1104 9 1.4826037379755093 1104 10-11 1.009957395154725 1104 12-13 1.000352514740328 1104 14-15 1.0961642861607999 1104 16-17 0.9111000040205894 1104 18-19 0.8304197669789346 1104 20-21 1.2204678807037226 1104 22-23 1.2117053267660722 1104 24-25 1.3846766736026694 1104 26-27 1.2668010759663666 1104 28-29 1.2818637112886755 1104 30-31 1.857173011669925 1104 32-33 1.831462281347509 1104 34-35 1.7294733266603615 1104 36-37 1.7026432855872713 1104 38-39 1.7623894770978552 1104 40-41 2.036070303156123 1104 42-43 1.8995338701076037 1104 44-45 1.5826660233082066 1104 46-47 1.6658594833145095 1104 48-49 1.5228073031535097 1104 50-51 2.002889805942946 1104 52-53 1.8379106047575249 1104 54-55 2.0468873251209025 1104 56-57 1.9994702582937904 1104 58-59 2.126211723760335 1104 60-61 2.2898733450826754 1104 62-63 2.1859051307240733 1104 64-65 2.335633300056603 1104 66-67 2.364234842707816 1104 68-69 2.5215642511619762 1104 70-71 2.327773471905825 1104 72-73 2.433307087469778 1104 74-75 2.443250331104313 1104 76-77 2.4124218060264724 1104 78-79 2.4375926248923427 1104 80-81 2.3697695262352774 1104 82-83 2.30876805740996 1104 84-85 2.435788722711006 1104 86-87 2.3169499920537646 1104 88-89 2.184607833335818 1104 90-91 2.160076702706064 1104 92-93 1.9735369187627931 1104 94-95 2.04502795766372 1104 96-97 2.0486256996815335 1104 98-99 2.2032859641640314 1104 100-101 2.0662169489160647 1104 102-103 1.930618915963688 1104 104-105 1.848079279342894 1104 106-107 2.0017289875145536 1104 108-109 1.8716776909522252 1104 110-111 2.019709609736129 1104 112-113 1.9859785986625411 1104 114-115 1.8351148563356077 1104 116-117 1.8841587745621453 1104 118-119 1.8374619053424155 1104 120-121 1.8697261741610447 1104 122-123 2.028369028663537 1104 124-125 1.657787034532877 1105 1 0.44674396239951264 1105 2 0.555133328533028 1105 3 0.3765463879822306 1105 4 0.6442143255732447 1105 5 0.6532593495672714 1105 6 0.851133139339975 1105 7 1.1195714889825474 1105 8 1.0118576522438971 1105 9 1.6411211186038628 1105 10-11 1.3509509755450715 1105 12-13 1.50403783568445 1105 14-15 1.3629357606937234 1105 16-17 1.1917553673923962 1105 18-19 1.5795165761288459 1105 20-21 1.6960554774887768 1105 22-23 1.665705946317722 1105 24-25 1.9093488521426352 1105 26-27 1.4964793386580872 1105 28-29 1.6184657136723324 1105 30-31 1.9423929547784446 1105 32-33 1.787480648349355 1105 34-35 1.9482929012197445 1105 36-37 1.7116380346256719 1105 38-39 1.7083060953549492 1105 40-41 1.8217403757659376 1105 42-43 1.857758054622181 1105 44-45 1.7681107186642535 1105 46-47 1.4734144338640114 1105 48-49 1.412152389786197 1105 50-51 1.4668331953768394 1105 52-53 1.5169609179879302 1105 54-55 1.7304813201517604 1105 56-57 1.559397164381032 1105 58-59 1.5917538791818906 1105 60-61 1.492601110996695 1105 62-63 1.385764799775309 1105 64-65 1.650391447638082 1105 66-67 2.062919954558936 1105 68-69 1.852395116288811 1105 70-71 1.760802006736629 1105 72-73 2.0075143068146986 1105 74-75 2.06152128654114 1105 76-77 1.7596092551736895 1105 78-79 1.8677213579978549 1105 80-81 1.906655463276465 1105 82-83 1.7401331665155944 1105 84-85 1.986803096491105 1105 86-87 1.931579661077727 1105 88-89 1.8560280134467106 1105 90-91 1.7890570266002115 1105 92-93 1.577592050223199 1105 94-95 1.4989758494153662 1105 96-97 1.5353960219302145 1105 98-99 1.6603695690909888 1105 100-101 1.6903634744722318 1105 102-103 1.6182344284824524 1105 104-105 1.252547926302089 1105 106-107 1.3665336582885352 1105 108-109 1.3007472657388846 1105 110-111 1.477092819931869 1105 112-113 1.3177794734894732 1105 114-115 1.3454363231866395 1105 116-117 1.427335342691464 1105 118-119 1.4187893095255504 1105 120-121 1.3691137248547314 1105 122-123 1.5242252940443741 1105 124-125 1.351884486871036 1106 1 0.00828001736250883 1106 2 -0.0029219576773265032 1106 3 0.004006752799948998 1106 4 -0.22315491769105478 1106 5 -0.1807494186549654 1106 6 0.17414744364204182 1106 7 0.32089148765653874 1106 8 0.04399985822253072 1106 9 0.7342803720618392 1106 10-11 0.2605789269469092 1106 12-13 0.22204635942411954 1106 14-15 0.34963373356030303 1106 16-17 0.24465942663177742 1106 18-19 0.3241006657006267 1106 20-21 0.22278798869768224 1106 22-23 0.07710743227447381 1106 24-25 0.054373631901135866 1106 26-27 0.11910858291689053 1106 28-29 0.0764395747565132 1106 30-31 0.5250363500197395 1106 32-33 0.26608583386604323 1106 34-35 0.2085974270478772 1106 36-37 0.21977329564188963 1106 38-39 0.3679894697116275 1106 40-41 0.4817371229046614 1106 42-43 0.45208119013182113 1106 44-45 0.4218747142552779 1106 46-47 0.39981948346496665 1106 48-49 0.3667288755357596 1106 50-51 0.5843433269001714 1106 52-53 0.3027558592706754 1106 54-55 0.8650161402706047 1106 56-57 0.5765539357193887 1106 58-59 0.6444274410857069 1106 60-61 0.6471933430810992 1106 62-63 0.7543839083670036 1106 64-65 0.932752237606838 1106 66-67 1.0512443580629096 1106 68-69 0.8737650681381766 1106 70-71 0.7281086790388258 1106 72-73 0.6420346237304031 1106 74-75 0.8736364336864959 1106 76-77 0.6584421616984741 1106 78-79 0.43931642027875384 1106 80-81 0.6166172991333561 1106 82-83 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-3.96513615983525 2216 94-95 -3.7836729234091244 2216 96-97 -3.9355891843871476 2216 98-99 -3.739524015320434 2216 100-101 -4.164795939911116 2216 102-103 -4.277902824985201 2216 104-105 -4.1390714542233376 2216 106-107 -4.2147671547195955 2216 108-109 -4.261564634693748 2216 110-111 -3.942550783637845 2216 112-113 -4.147302802654622 2216 114-115 -3.8625508546928558 2216 116-117 -3.897192579644315 2216 118-119 -4.046062196315987 2216 120-121 -4.038362187950668 2216 122-123 -4.061124013818887 2216 124-125 -4.039639102208042 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 100879.0 3 33090.0 4 15424.0 5 13130.0 6 14556.0 7 17349.0 8 19226.0 9 19699.0 10 21152.0 11 23038.0 12 25774.0 13 28338.0 14 30467.0 15 24708.0 16 16130.0 17 13420.0 18 11761.0 19 11014.0 20 10592.0 21 11256.0 22 13415.0 23 17935.0 24 24303.0 25 32126.0 26 38787.0 27 42751.0 28 48062.0 29 54428.0 30 59968.0 31 68671.0 32 79586.0 33 94741.0 34 115082.0 35 144769.0 36 196638.0 37 250755.0 38 91406.0 39 438.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6820905366767 20.34197434483195 14.24907651542305 22.726858603068298 2 15.776953010038913 21.4770609201792 38.94231712501226 23.80366894476963 3 18.894588372762232 26.831961387873974 30.13890947775966 24.13454076160413 4 12.21294557050498 18.050028394198847 37.12421369910886 32.61281233618731 5 11.766103645828792 40.2379140906356 33.940662632216885 14.055319631318724 6 29.880307205329657 38.76821388397178 16.911771117641415 14.43970779305714 7 26.310561254754177 34.90750069786105 20.93119744233923 17.850740605045534 8 26.084767045405 35.92015539026326 19.21308609248424 18.781991471847505 9 26.299932856059087 16.021078401451007 20.460535494728767 37.21845324776114 10-11 24.47763683139599 27.880842037683014 26.616820861118175 21.02470026980282 12-13 25.68767714279252 25.645875463149803 27.71688436331055 20.949563030747125 14-15 22.448297784270558 26.3784373563411 26.707754303669738 24.465510555718602 16-17 20.720419974743525 29.728926185580047 27.67469834208735 21.875955497589082 18-19 20.513413033354162 27.224385143433604 31.494215975956468 20.767985847255765 20-21 21.39943623160286 26.255970691019602 31.902047966095417 20.442545111282126 22-23 21.207673611801834 25.978760470636708 31.92709311226332 20.886472805298137 24-25 20.82206713121299 26.390409670993286 31.913106038102974 20.87441715969075 26-27 20.535710879305768 26.707578087146285 31.93638790981138 20.820323123736564 28-29 20.484560549357926 26.42178574027147 32.303037421316205 20.7906162890544 30-31 20.568297755454402 26.26100363626851 32.02573183173756 21.14496677653953 32-33 20.431541520243464 26.670777779715554 31.89819517958824 20.999485520452744 34-35 20.930861217485898 26.53710394675063 31.73181181886035 20.80022301690312 36-37 21.139858363826423 26.18236338605722 31.602748356534242 21.075029893582116 38-39 20.83342303163548 26.46888350002684 31.59552978178924 21.10216368654844 40-41 20.91517641208009 26.48343518158965 31.5179409620743 21.083447444255963 42-43 20.90822584055826 26.649778837748222 30.960289985241317 21.481705336452205 44-45 21.04794311692254 26.635534265307033 30.93209450465065 21.38442811311978 46-47 21.424205587179195 27.116161083547723 30.072406009935897 21.387227319337182 48-49 21.535864404008475 27.393605787899638 29.35312591542963 21.717403892662258 50-51 21.516100443453603 27.351684848346064 29.171352139560714 21.960862568639623 52-53 21.76798934416938 27.54165976333941 28.9710300054936 21.71932088699762 54-55 21.42863575027663 27.345028058882036 28.999690281243733 22.226645909597597 56-57 21.47987352710112 27.67645152122002 28.768224488033006 22.075450463645854 58-59 21.479575597973476 27.26978675258766 28.99073173815607 22.2599059112828 60-61 21.428618142816035 27.4305888610818 29.304951211642933 21.835841784459227 62-63 21.25250837396327 27.490007379333726 29.453678603311218 21.80380564339179 64-65 21.11332765805213 27.210537481124053 29.2020758222659 22.474059038557918 66-67 21.095301179202853 27.56930474461508 28.844484787029312 22.490909289152754 68-69 21.22113199608576 27.350336283002562 29.15755255844043 22.270979162471246 70-71 21.271079492950363 27.232158307356535 29.26209545890434 22.234666740788764 72-73 21.298269085451103 27.34882074577702 28.805507249617605 22.547402919154266 74-75 21.091315281455167 27.7655813811529 28.66846596823826 22.474637369153676 76-77 21.21747300788651 27.41141401041997 28.917920158226202 22.45319282346731 78-79 21.234959499138206 27.50396152247815 28.77352899098694 22.487549987396704 80-81 21.234088031736466 27.768561215263716 28.54317030009358 22.454180452906243 82-83 21.423458161033864 27.545715278392795 28.583013713561645 22.447812847011697 84-85 21.12778416487112 27.485586802506017 28.470918953916737 22.915710078706127 86-87 21.065764654652313 27.57129831633023 28.55584155966038 22.80709546935708 88-89 21.139891374813573 27.459156553849475 28.45913393609203 22.941818135244922 90-91 21.1209359626718 27.565107846132086 28.569816926037117 22.744139265158996 92-93 21.272341750955064 27.538101117738158 28.413608626234215 22.775948505072563 94-95 21.020812423850423 27.204696332312057 28.6791946886666 23.095296555170922 96-97 20.97369969313175 27.15392981298232 28.633361246962586 23.23900924692335 98-99 20.767370224714202 27.295374480070457 28.38921576749012 23.548039527725216 100-101 20.768960152688937 27.235322506058424 28.5405306125897 23.455186728662937 102-103 20.855858040795994 26.8363620310687 28.616356657294357 23.691423270840954 104-105 20.732399202118962 27.057949925334903 28.650550995716294 23.55909987682984 106-107 20.60431198288047 27.046235319412045 28.6743400803384 23.675112617369084 108-109 20.73644150193148 26.981591006199913 28.17579395530132 24.106173536567287 110-111 20.499814563559465 27.13811213441022 28.438510720569766 23.92356258146055 112-113 20.510212896177723 27.197366122729154 28.476236489784107 23.816184491309016 114-115 20.290572656925505 27.282007150956243 28.209980110193722 24.21744008192453 116-117 20.205366719277286 27.211771553641036 28.37022253420417 24.21263919287751 118-119 19.99926968989616 27.049160225131715 28.374536811716787 24.577033273255335 120-121 19.93123036764067 27.24030648212919 28.378945853860987 24.449517296369155 122-123 20.00878548803059 27.17476254112066 28.245725522169735 24.570726448679014 124-125 19.817239178434757 27.07442062514001 28.13947619574501 24.96886400068022 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1087.0 1 815.0 2 766.5 3 1068.5 4 821.0 5 603.5 6 1221.5 7 2653.0 8 4175.0 9 4837.5 10 3965.0 11 2705.0 12 2328.5 13 2353.5 14 2567.0 15 2806.5 16 3145.0 17 3519.5 18 3841.5 19 4302.0 20 4849.0 21 5578.5 22 6490.5 23 7444.0 24 8479.0 25 10359.5 26 13267.0 27 16402.5 28 19221.0 29 22578.0 30 26904.0 31 31899.5 32 37625.5 33 42151.0 34 45693.0 35 49119.0 36 53444.5 37 58137.5 38 58784.0 39 58063.5 40 58287.0 41 58521.0 42 58020.0 43 58625.5 44 60438.0 45 61308.0 46 60630.0 47 59547.5 48 61062.0 49 60643.0 50 57946.5 51 55228.0 52 53051.0 53 52617.5 54 51214.5 55 47258.0 56 42945.0 57 39863.0 58 38022.5 59 34733.0 60 29488.5 61 24508.5 62 20104.0 63 15719.5 64 11638.0 65 8864.5 66 6897.5 67 5503.5 68 4083.0 69 2747.5 70 2053.5 71 1676.5 72 1366.0 73 1172.0 74 986.0 75 780.5 76 539.5 77 350.5 78 257.5 79 172.5 80 140.5 81 103.5 82 68.0 83 50.0 84 37.0 85 25.5 86 13.5 87 10.0 88 8.0 89 4.0 90 1.0 91 1.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10142441074651855 2 2.1799980816016883E-4 3 0.0 4 0.19096783194830788 5 0.0 6 0.0 7 0.037495967003549034 8 0.0 9 0.0 10-11 2.997497362202321E-4 12-13 5.17749544380401E-4 14-15 2.997497362202321E-4 16-17 0.004741495827483672 18-19 0.0013897487770210763 20-21 0.00370599673872287 22-23 0.0016622485372212873 24-25 0.005395495251964178 26-27 0.004741495827483672 28-29 0.003678746762702849 30-31 8.719992326406753E-4 32-33 0.0 34-35 0.0013897487770210763 36-37 0.0011989989448809284 38-39 5.17749544380401E-4 40-41 0.0014442487290611183 42-43 6.539994244805065E-4 44-45 6.812494005005276E-4 46-47 1.362498801001055E-4 48-49 8.174992806006331E-5 50-51 0.04019371462953113 52-53 0.0 54-55 0.1393291273903679 56-57 2.452497841801899E-4 58-59 0.0 60-61 0.0 62-63 5.44999520400422E-4 64-65 0.06436444335928984 66-67 0.03128297247098422 68-69 0.0013897487770210763 70-71 0.002915747434142258 72-73 0.07376568508619712 74-75 0.004250996259123292 76-77 0.0012262489209009496 78-79 0.0014442487290611183 80-81 1.6349985612012662E-4 82-83 8.174992806006331E-5 84-85 2.7249976020021104E-5 86-87 1.6349985612012662E-4 88-89 0.0012262489209009496 90-91 2.452497841801899E-4 92-93 0.0023162479617017937 94-95 0.0038422466188229756 96-97 0.0020437482015015825 98-99 0.0 100-101 0.0012262489209009496 102-103 0.0014714987050811396 104-105 2.1799980816016883E-4 106-107 2.7249976020021104E-5 108-109 0.0 110-111 1.907498321401477E-4 112-113 1.362498801001055E-4 114-115 2.452497841801899E-4 116-117 0.0 118-119 0.0014714987050811396 120-121 0.0023162479617017937 122-123 0.001089999040800844 124-125 0.009319491798847217 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1834864.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.171941953823016 #Duplication Level Percentage of deduplicated Percentage of total 1 76.5780803169282 36.123367596453335 2 10.30600315307838 9.723083650258605 3 4.463557769957264 6.316640640958791 4 2.3939479976713343 4.51708703946492 5 1.468294889219165 3.4631160642670724 6 1.005036608944295 2.844571714715244 7 0.6910976923186481 2.2820294158533416 8 0.4919395446126124 1.8564594914605024 9 0.3851507080123 1.6351476157647045 >10 1.9891426275262647 16.83811713095843 >50 0.1458549957647691 4.779591695409939 >100 0.07709787432965894 6.557318504863457 >500 0.003836657309658862 1.2337921541974004 >1k 5.994777046341972E-4 0.4552842925184081 >5k 3.5968662278051836E-4 1.37439299285588 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9580 0.5221095405436044 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9178 0.5002005598235074 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5543 0.30209323415795397 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2491 0.13575938053174513 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2171 0.11831939587893163 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 5.449995204004221E-5 0.0 0.0 0.0 3 0.0 5.449995204004221E-5 0.0 0.0 0.0 4 0.0 5.449995204004221E-5 0.0 0.0 0.0 5 0.0 5.449995204004221E-5 0.0 0.0 0.0 6 0.0 1.0899990408008441E-4 0.0 0.0 0.0 7 0.0 1.0899990408008441E-4 0.0 0.0 0.0 8 0.0 1.0899990408008441E-4 0.0 0.0 0.0 9 0.0 1.0899990408008441E-4 0.0 0.0 0.0 10-11 0.0 1.6349985612012662E-4 0.0 0.0 0.0 12-13 0.0 1.6349985612012662E-4 0.0 0.0 0.0 14-15 0.0 1.6349985612012662E-4 0.0 0.0 0.0 16-17 0.0 1.6349985612012662E-4 0.0 1.3624988010010552E-4 0.0 18-19 0.0 1.6349985612012662E-4 0.0 1.9074983214014774E-4 0.0 20-21 0.0 1.6349985612012662E-4 0.0 4.3599961632033766E-4 0.0 22-23 0.0 1.6349985612012662E-4 0.0 0.0011717489688609075 0.0 24-25 0.0 1.6349985612012662E-4 0.0 0.001607748585181245 0.0 26-27 0.0 1.6349985612012662E-4 0.0 0.002152748105581667 0.0 28-29 0.0 1.6349985612012662E-4 0.0 0.0034062470025026377 0.0 30-31 0.0 1.6349985612012662E-4 0.0 0.0076299932856059086 0.0 32-33 0.0 1.6349985612012662E-4 0.0 0.015913985995692322 0.0 34-35 0.0 1.9074983214014774E-4 0.0 0.026677726523600658 0.0 36-37 0.0 2.1799980816016883E-4 0.0 0.04504421036109488 0.0 38-39 0.0 2.1799980816016883E-4 0.0 0.07766243165706013 0.0 40-41 0.0 2.1799980816016883E-4 0.0 0.11742014667027093 0.0 42-43 0.0 2.1799980816016883E-4 0.0 0.15039261765449646 0.0 44-45 0.0 2.1799980816016883E-4 0.0 0.1900958327156672 0.0 46-47 0.0 2.1799980816016883E-4 0.0 0.24178903722564724 0.0 48-49 0.0 2.1799980816016883E-4 0.0 0.3022839839900941 0.0 50-51 0.0 2.1799980816016883E-4 0.0 0.3689374253350657 0.0 52-53 0.0 2.1799980816016883E-4 0.0 0.4347733673994367 0.0 54-55 0.0 2.1799980816016883E-4 0.0 0.50366130677805 0.0 56-57 0.0 2.7249976020021104E-4 0.0 0.5891172315768363 0.0 58-59 0.0 2.7249976020021104E-4 0.0 0.6874351450570724 0.0 60-61 0.0 2.7249976020021104E-4 0.0 0.7998140461636394 0.0 62-63 0.0 2.7249976020021104E-4 0.0 0.9082689507233234 0.0 64-65 0.0 2.7249976020021104E-4 0.0 1.0194488528850094 0.0 66-67 0.0 2.7249976020021104E-4 0.0 1.1508754872295712 0.0 68-69 0.0 2.7249976020021104E-4 0.0 1.302957603397309 0.0 70-71 0.0 2.7249976020021104E-4 0.0 1.488911439757933 0.0 72-73 0.0 2.7249976020021104E-4 0.0 1.6690882812023125 0.0 74-75 0.0 2.7249976020021104E-4 0.0 1.8458861256202095 0.0 76-77 0.0 2.7249976020021104E-4 0.0 2.036308958048117 0.0 78-79 0.0 2.7249976020021104E-4 0.0 2.266217005729035 0.0 80-81 0.0 2.7249976020021104E-4 0.0 2.532122271732401 0.0 82-83 0.0 2.7249976020021104E-4 0.0 2.8084915285274548 0.0 84-85 0.0 2.7249976020021104E-4 0.0 3.0781300412455637 0.0 86-87 0.0 2.7249976020021104E-4 0.0 3.359867543316562 0.0 88-89 0.0 2.7249976020021104E-4 0.0 3.6830250089379923 0.0 90-91 0.0 2.7249976020021104E-4 0.0 4.060355426887225 5.449995204004221E-5 92-93 0.0 2.7249976020021104E-4 0.0 4.4689143173554005 5.449995204004221E-5 94-95 0.0 2.7249976020021104E-4 0.0 4.870633463842552 5.449995204004221E-5 96-97 0.0 2.7249976020021104E-4 0.0 5.274450858483244 5.449995204004221E-5 98-99 0.0 2.7249976020021104E-4 0.0 5.70641747835262 5.449995204004221E-5 100-101 0.0 2.7249976020021104E-4 0.0 6.21122328412351 5.449995204004221E-5 102-103 0.0 2.7249976020021104E-4 0.0 6.75360680682601 5.449995204004221E-5 104-105 0.0 2.7249976020021104E-4 0.0 7.29623557931269 5.449995204004221E-5 106-107 0.0 2.7249976020021104E-4 0.0 7.82477611419702 5.449995204004221E-5 108-109 0.0 2.7249976020021104E-4 0.0 8.375607129465726 5.449995204004221E-5 110-111 0.0 2.7249976020021104E-4 0.0 9.003419326990993 5.449995204004221E-5 112-113 0.0 2.7249976020021104E-4 0.0 9.67052598993713 5.449995204004221E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8080 0.0 52.782967 1 GTATCAA 12915 0.0 43.321907 1 GTACATG 10080 0.0 35.327503 1 TATCAAC 16320 0.0 34.028595 2 ATCAACG 16155 0.0 33.158966 3 TACATGG 10325 0.0 33.126087 2 CAACGCA 16200 0.0 32.60884 5 TCAACGC 16520 0.0 32.173855 4 AACGCAG 16390 0.0 32.049343 6 ACATGGG 10295 0.0 30.155022 3 GAGTACT 12175 0.0 28.209932 12-13 CGCAGAG 19145 0.0 27.582972 8 ACGCAGA 18965 0.0 27.384119 7 GTACTTT 13260 0.0 26.238037 14-15 CATGGGA 6090 0.0 26.124157 4 CCACGCA 480 0.0 26.026478 6 GCAGAGT 19585 0.0 25.900547 9 CATGGGG 4195 0.0 25.567533 4 GTATCAC 645 0.0 24.969118 1 ACCGCAG 535 0.0 24.462807 6 >>END_MODULE