FastQCFastQC Report
Thu 26 May 2016
SRR1364490_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364490_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014469
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44400.050.410481
GGTATCA27150.049.5512431
ATCAACG52100.041.002553
TCAACGC53300.039.5742844
CAACGCA54200.039.027085
TATCAAC58350.038.0383872
AACGCAG55750.036.7444236
ACGCAGA65200.031.0534027
GTACATG68950.030.9938951
CGCAGAG63300.030.6699688
TACATGG68800.030.0985032
ACATGGG69650.027.9370633
GCAGAGT71250.025.1582879
CGCATAG2451.607259E-824.3071128
AACGCAT3204.965841E-1022.3310496
AGAGTAC69450.021.59577210-11
CAGAGTA73150.021.31706410-11
CATGGGG34350.021.1626514
GTGTAGC12800.020.46251
CATGGGA40500.020.303044
Produced by FastQC (version 0.11.5)