##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364490_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2014469 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.055622598312507 30.0 26.0 31.0 7.0 34.0 2 26.254644275985385 30.0 26.0 31.0 7.0 34.0 3 26.31587530014113 31.0 26.0 31.0 7.0 34.0 4 29.12462440474388 35.0 28.0 37.0 7.0 37.0 5 29.708617506648153 35.0 29.0 37.0 7.0 37.0 6 29.8105892917687 35.0 30.0 37.0 8.0 37.0 7 29.66138868356872 35.0 30.0 37.0 2.0 37.0 8 29.320716278086184 35.0 28.0 37.0 2.0 37.0 9 29.960305172231493 35.0 27.0 39.0 2.0 39.0 10-11 30.02619896359785 35.0 27.5 39.0 2.0 39.0 12-13 29.866430309922862 35.0 27.0 39.0 2.0 39.0 14-15 30.516691495376698 36.0 27.0 40.0 2.0 41.0 16-17 30.751839566655033 36.0 27.0 40.0 2.0 41.0 18-19 30.683563013379704 36.5 27.0 40.0 2.0 41.0 20-21 30.470185443409655 36.0 27.0 40.0 2.0 41.0 22-23 30.518742408048972 36.0 27.0 40.0 2.0 41.0 24-25 30.53628524439939 36.0 27.0 40.0 2.0 41.0 26-27 30.642438528465814 36.5 27.5 40.0 2.0 41.0 28-29 30.58787278434168 36.0 28.0 40.0 2.0 41.0 30-31 30.25273409518836 36.0 27.0 40.0 2.0 41.0 32-33 30.26980037915699 36.0 27.0 40.0 2.0 41.0 34-35 29.990022929119284 36.0 27.0 40.0 2.0 41.0 36-37 29.765621610459135 36.0 26.0 40.0 2.0 41.0 38-39 29.62900446718217 35.0 26.0 40.0 2.0 41.0 40-41 29.690660913620412 35.0 26.5 40.0 2.0 41.0 42-43 29.558671788942892 35.0 26.0 40.0 2.0 41.0 44-45 29.23608032687522 35.0 25.5 40.0 2.0 41.0 46-47 28.998951336555688 35.0 24.5 40.0 2.0 41.0 48-49 28.777756321889292 35.0 24.0 39.5 2.0 41.0 50-51 28.7787265527541 35.0 24.0 39.0 2.0 41.0 52-53 28.584450294345558 35.0 24.0 39.0 2.0 41.0 54-55 28.469336088070854 34.5 23.5 39.0 2.0 41.0 56-57 28.07633128134511 34.0 23.0 39.0 2.0 40.5 58-59 27.858218220285345 34.0 22.0 39.0 2.0 40.0 60-61 27.460589118025645 33.5 20.5 38.5 2.0 40.0 62-63 27.407021651859623 33.0 21.5 38.0 2.0 40.0 64-65 27.224175700891898 33.0 21.0 38.0 2.0 40.0 66-67 27.008678465640326 33.0 21.0 38.0 2.0 40.0 68-69 26.83175640826441 33.0 20.5 37.0 2.0 40.0 70-71 26.715688104408656 33.0 20.5 37.0 2.0 40.0 72-73 26.680496448443733 33.0 20.5 36.5 2.0 39.0 74-75 26.394138107858694 33.0 20.0 36.0 2.0 39.0 76-77 26.1228916900682 33.0 19.0 36.0 2.0 39.0 78-79 25.807216690850044 32.0 18.0 35.0 2.0 37.5 80-81 25.789011397048057 33.0 19.0 35.0 2.0 37.0 82-83 25.58395388561452 32.5 18.0 35.0 2.0 37.0 84-85 25.380987495960476 32.0 18.0 35.0 2.0 36.0 86-87 25.179193623729134 32.0 17.0 35.0 2.0 36.0 88-89 24.959798090712738 32.0 13.5 35.0 2.0 36.0 90-91 24.788656216600998 32.0 11.0 35.0 2.0 35.0 92-93 24.67964783771803 32.0 9.5 35.0 2.0 35.0 94-95 24.599518781376133 32.0 7.0 35.0 2.0 35.0 96-97 24.473596267800595 32.0 7.0 35.0 2.0 35.0 98-99 24.3683878977537 32.0 6.0 35.0 2.0 35.0 100-101 24.319888516527186 32.0 2.0 35.0 2.0 35.0 102-103 23.184206110890763 30.0 2.0 34.0 2.0 35.0 104-105 23.960767328760085 31.5 2.0 34.5 2.0 35.0 106-107 24.064451972207067 32.0 2.0 35.0 2.0 35.0 108-109 24.006597768444188 32.0 2.0 35.0 2.0 35.0 110-111 23.89193628693219 32.0 2.0 35.0 2.0 35.0 112-113 23.86994885500844 32.0 2.0 35.0 2.0 35.0 114-115 23.819466072697075 32.0 2.0 35.0 2.0 35.0 116-117 23.646348491835816 32.0 2.0 35.0 2.0 35.0 118-119 23.493776275534643 32.0 2.0 35.0 2.0 35.0 120-121 23.349475717918715 32.0 2.0 35.0 2.0 35.0 122-123 23.08221918530392 32.0 2.0 35.0 2.0 35.0 124-125 22.105124725175717 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6656217828930124 1101 2 1.3441287777074322 1101 3 1.6740677867345575 1101 4 2.057379055519288 1101 5 1.5639296097464133 1101 6 1.4946101196749737 1101 7 1.4113670929607807 1101 8 1.73562968355969 1101 9 2.198439819045369 1101 10-11 2.27476827109626 1101 12-13 1.988471243596095 1101 14-15 2.3386822017321123 1101 16-17 1.836156529270621 1101 18-19 1.9311436699955387 1101 20-21 2.31014377725009 1101 22-23 2.1890034353839916 1101 24-25 2.048000457801624 1101 26-27 1.7946230913781385 1101 28-29 1.6755264340838316 1101 30-31 1.8256757851400316 1101 32-33 2.0241514709543544 1101 34-35 2.3948072255260158 1101 36-37 2.2627129708011466 1101 38-39 2.3243403737197355 1101 40-41 2.0454808201386356 1101 42-43 2.155842599678383 1101 44-45 2.3449906445158852 1101 46-47 2.329479048882142 1101 48-49 2.314176155026267 1101 50-51 2.1757077195560797 1101 52-53 2.1254920147724086 1101 54-55 2.210130353317144 1101 56-57 2.4194041721906494 1101 58-59 2.4824227997306387 1101 60-61 2.6277195561230897 1101 62-63 2.2648846437060044 1101 64-65 2.1895191633220783 1101 66-67 2.3082031472518345 1101 68-69 2.130057660739901 1101 70-71 2.147779483154604 1101 72-73 2.1001628742048624 1101 74-75 2.153024260277302 1101 76-77 2.2233510960335927 1101 78-79 2.1365474161990186 1101 80-81 2.0215806247669903 1101 82-83 2.019497404133631 1101 84-85 1.8639094545441033 1101 86-87 1.8436640973225167 1101 88-89 2.0058765375154444 1101 90-91 2.0150526444318295 1101 92-93 2.0315753558412304 1101 94-95 1.9883371747430019 1101 96-97 2.0177778156424075 1101 98-99 1.954471075630046 1101 100-101 1.9693985657006152 1101 102-103 1.9340311333180331 1101 104-105 1.8525177071303105 1101 106-107 1.9538130086602976 1101 108-109 2.069036353560307 1101 110-111 2.153141444787863 1101 112-113 2.0560138910154038 1101 114-115 2.014717797174818 1101 116-117 2.1364720418378873 1101 118-119 2.0986839136656137 1101 120-121 2.1379097880683418 1101 122-123 2.125427712177011 1101 124-125 2.196987311257267 1102 1 0.9363227152547218 1102 2 0.8781447225007604 1102 3 0.8615208911487926 1102 4 1.3500011370106293 1102 5 1.256574224652514 1102 6 1.0428500619078491 1102 7 1.0884317832763521 1102 8 1.1495336045307454 1102 9 1.4928821258551643 1102 10-11 1.166119165980728 1102 12-13 1.732735714892371 1102 14-15 1.9317103945836323 1102 16-17 1.401451216915131 1102 18-19 1.6973628748189888 1102 20-21 1.703608233877091 1102 22-23 1.7044299503688087 1102 24-25 1.3697880635998345 1102 26-27 1.382638285842095 1102 28-29 1.1345455755234681 1102 30-31 1.0238165234751158 1102 32-33 0.9712884035153131 1102 34-35 1.339288392180329 1102 36-37 1.6633927288130579 1102 38-39 1.671112466958263 1102 40-41 1.3114870459505141 1102 42-43 1.266585066869613 1102 44-45 1.6270985542739744 1102 46-47 1.4383802576066707 1102 48-49 1.6318724948074106 1102 50-51 1.3364567663475313 1102 52-53 1.5192844708440134 1102 54-55 1.2807513793268654 1102 56-57 1.451951571787518 1102 58-59 1.4826047928454962 1102 60-61 1.9525899154457491 1102 62-63 1.7653391852382825 1102 64-65 1.737837894061304 1102 66-67 1.7272830857663983 1102 68-69 1.5313249250813712 1102 70-71 1.7942256949718391 1102 72-73 1.596771830619474 1102 74-75 1.6804489851401883 1102 76-77 1.589683232683754 1102 78-79 1.4641321792961506 1102 80-81 1.0103585331562073 1102 82-83 1.1089618756652406 1102 84-85 1.186475973223697 1102 86-87 1.2169221211989196 1102 88-89 0.9631959162129142 1102 90-91 0.9096387709084155 1102 92-93 0.8626486308191019 1102 94-95 1.2140214335544677 1102 96-97 1.2464716777284934 1102 98-99 1.3259804065033336 1102 100-101 1.2000100233438715 1102 102-103 0.9687660293603493 1102 104-105 1.0726165129181595 1102 106-107 1.352462602097873 1102 108-109 1.399789375567746 1102 110-111 1.0526071145715576 1102 112-113 1.1509704166256398 1102 114-115 1.1194923414369953 1102 116-117 1.269110408575191 1102 118-119 1.3693639109357214 1102 120-121 1.3045911632302847 1102 122-123 1.5726261262482168 1102 124-125 1.4045619313760582 1103 1 1.4980759356405642 1103 2 1.2717852016963747 1103 3 1.3390410232236896 1103 4 1.9640377662733535 1103 5 1.26074702080588 1103 6 1.3259503700250015 1103 7 1.3267821587425956 1103 8 1.410778054612706 1103 9 2.258190887048272 1103 10-11 1.6973304108038754 1103 12-13 1.7895939527261397 1103 14-15 1.9801144078036828 1103 16-17 1.903030975118174 1103 18-19 1.615869549250764 1103 20-21 2.0413787199764784 1103 22-23 1.9375551756838938 1103 24-25 1.9016835005127852 1103 26-27 1.6195523389846507 1103 28-29 1.5107929571559815 1103 30-31 1.6813647850833071 1103 32-33 1.7523570469594425 1103 34-35 1.5629079715607475 1103 36-37 1.966424635393004 1103 38-39 2.139206623335376 1103 40-41 2.1156941744290094 1103 42-43 1.9772571773582435 1103 44-45 1.9989073268555515 1103 46-47 2.126759820948976 1103 48-49 2.2157062501688323 1103 50-51 2.059629200818634 1103 52-53 2.116245505373083 1103 54-55 1.969653192607712 1103 56-57 2.0825382569323168 1103 58-59 2.2289592536326204 1103 60-61 2.2730871774992707 1103 62-63 1.910835151361887 1103 64-65 1.9704005679809882 1103 66-67 1.9996825962190563 1103 68-69 1.8429320588221216 1103 70-71 1.9855131974164344 1103 72-73 1.9418213685222838 1103 74-75 1.923351019907244 1103 76-77 1.8824404276473743 1103 78-79 2.117260485390087 1103 80-81 1.6839956174081436 1103 82-83 1.7930641830488234 1103 84-85 1.7281368802018484 1103 86-87 1.7134268902210081 1103 88-89 1.8142731972262816 1103 90-91 1.7257470030479496 1103 92-93 1.7279291073600227 1103 94-95 1.5866255340338071 1103 96-97 1.7609410297713914 1103 98-99 1.7562611932820218 1103 100-101 1.5425289635559487 1103 102-103 1.5503518371998588 1103 104-105 1.7251986630936642 1103 106-107 1.6995783137165539 1103 108-109 1.9626653915964347 1103 110-111 2.0276270345851906 1103 112-113 1.8093380593762447 1103 114-115 1.7945341842650464 1103 116-117 1.9236767550817184 1103 118-119 1.9461750024464344 1103 120-121 1.907654407751842 1103 122-123 1.9341018212789933 1103 124-125 1.763873325270417 1104 1 1.5500595317646955 1104 2 1.343038464975372 1104 3 1.4082917392984555 1104 4 1.7320367224717366 1104 5 1.3008088766836607 1104 6 1.134132144457908 1104 7 1.4732958314992217 1104 8 1.6855773895342487 1104 9 2.297272173751999 1104 10-11 2.066412814800618 1104 12-13 1.4625883091840208 1104 14-15 1.8826808149251093 1104 16-17 1.5477591241124635 1104 18-19 1.0402050770047673 1104 20-21 -3.4668703158686753 1104 22-23 -0.2385798186792769 1104 24-25 1.1647039277626483 1104 26-27 1.3123474964614097 1104 28-29 0.9139310676288375 1104 30-31 1.663765927967738 1104 32-33 1.5968407717867699 1104 34-35 1.7247182996009585 1104 36-37 1.881568283087951 1104 38-39 1.9494744621586761 1104 40-41 2.0693793481186376 1104 42-43 2.07461979694963 1104 44-45 2.135491454356032 1104 46-47 1.9978340468831561 1104 48-49 2.1449431315820746 1104 50-51 2.1056977190456223 1104 52-53 2.1532044483057113 1104 54-55 2.0361405203785807 1104 56-57 2.3075812605889787 1104 58-59 2.2246285307981353 1104 60-61 2.4388163781292818 1104 62-63 2.1139424663295863 1104 64-65 2.2347172136696543 1104 66-67 2.045995277476063 1104 68-69 2.0972895015603896 1104 70-71 2.263352819051409 1104 72-73 2.1673808637222116 1104 74-75 2.2795112027926265 1104 76-77 2.214574753873059 1104 78-79 2.0258216638040594 1104 80-81 1.8985319631922373 1104 82-83 1.8553144242274264 1104 84-85 1.84966226681507 1104 86-87 1.767995508799185 1104 88-89 1.9212279358543292 1104 90-91 1.9698213397787967 1104 92-93 1.6960192469787465 1104 94-95 1.753816308737548 1104 96-97 1.8769760124207089 1104 98-99 1.8535436065304722 1104 100-101 1.4308890553418507 1104 102-103 1.7313990503838426 1104 104-105 1.906918676926459 1104 106-107 2.0650699450240353 1104 108-109 2.1128298985363365 1104 110-111 2.141101709525671 1104 112-113 2.0412613216778475 1104 114-115 1.8742646984138176 1104 116-117 1.9862787383794007 1104 118-119 1.8894993818918273 1104 120-121 1.867570728287724 1104 122-123 1.737383641000502 1104 124-125 1.8498061213082977 1105 1 1.2324570324831434 1105 2 0.892649008982545 1105 3 0.9670511350324418 1105 4 1.407609747940871 1105 5 1.2010803596954283 1105 6 0.9932249057084128 1105 7 0.989897203486553 1105 8 1.3021146567691737 1105 9 1.6472133882093907 1105 10-11 1.3574334969285147 1105 12-13 1.38314134813605 1105 14-15 1.6586421285250168 1105 16-17 1.488104635394432 1105 18-19 1.069719031727086 1105 20-21 1.2213566605345534 1105 22-23 1.1395227581918164 1105 24-25 1.2584354494188297 1105 26-27 1.1470987971618705 1105 28-29 0.7220565790413076 1105 30-31 1.1709969799731574 1105 32-33 1.081654073196649 1105 34-35 1.0099882303491015 1105 36-37 1.2354572968534967 1105 38-39 1.1400652008328507 1105 40-41 1.298050286102896 1105 42-43 1.5548785781612189 1105 44-45 1.8000304528637194 1105 46-47 1.9432791437017585 1105 48-49 1.5843200265801158 1105 50-51 1.5679344973576939 1105 52-53 1.5282287393637937 1105 54-55 1.5194174448710491 1105 56-57 1.906463479477722 1105 58-59 1.6340494329190953 1105 60-61 2.0329721126771645 1105 62-63 1.8796160499233068 1105 64-65 1.658193926736537 1105 66-67 1.8199801145602557 1105 68-69 1.4269841013756661 1105 70-71 1.5387130855849556 1105 72-73 1.495454404800082 1105 74-75 1.8247355027586103 1105 76-77 1.693522645380849 1105 78-79 1.5454616521197657 1105 80-81 1.5365458355022668 1105 82-83 1.6173758151899165 1105 84-85 1.5327082383103487 1105 86-87 1.6947122116569702 1105 88-89 1.6850770068460612 1105 90-91 1.7122718207724823 1105 92-93 1.7445443884957328 1105 94-95 1.614683321967675 1105 96-97 1.741505100649519 1105 98-99 1.6248232589974165 1105 100-101 1.666357848885493 1105 102-103 1.6321226898694583 1105 104-105 1.674077383896062 1105 106-107 1.769784485965829 1105 108-109 1.8273069152473553 1105 110-111 1.8090932298068942 1105 112-113 1.7228451015017114 1105 114-115 1.6637650321764994 1105 116-117 1.797144281143531 1105 118-119 1.852755131673927 1105 120-121 1.7417690524482161 1105 122-123 1.9111112080015076 1105 124-125 1.6889071171683945 1106 1 -0.5399799027585495 1106 2 -0.22259163128230242 1106 3 -0.2946132274576776 1106 4 -0.6533490844482515 1106 5 -1.1188098937536886 1106 6 -0.4432109260014343 1106 7 -0.458630532024376 1106 8 0.020995329274462904 1106 9 0.09108115526525751 1106 10-11 -2.3904136306816532 1106 12-13 -0.5736704108148842 1106 14-15 -0.2264596216703083 1106 16-17 -0.40283219397620584 1106 18-19 -3.2433599747387767 1106 20-21 -5.64053812605027 1106 22-23 -6.584549556373492 1106 24-25 -6.204566385164217 1106 26-27 -5.038804443265104 1106 28-29 -1.7641976683584737 1106 30-31 -3.73032641783848 1106 32-33 -4.206924913176895 1106 34-35 -3.4664970458468325 1106 36-37 -5.83695734364473 1106 38-39 -6.300622136061975 1106 40-41 -2.5791926153203377 1106 42-43 -1.2216934137678521 1106 44-45 -0.5032133646918631 1106 46-47 -0.2425453696198332 1106 48-49 -0.1706399259556477 1106 50-51 -0.14185821755474848 1106 52-53 -0.26971616441332813 1106 54-55 -0.288249193846692 1106 56-57 -0.12340432853555683 1106 58-59 0.18810974232531308 1106 60-61 0.35992921055843397 1106 62-63 0.029800802637122814 1106 64-65 -0.08082146861456607 1106 66-67 -0.28380032240126596 1106 68-69 -0.13383712762496458 1106 70-71 0.03169473318581595 1106 72-73 0.012230233279701963 1106 74-75 -0.06450825915671032 1106 76-77 -0.12104261291747065 1106 78-79 -0.1314751022910805 1106 80-81 -0.3943571711583367 1106 82-83 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-5.023565553620561 2216 86-87 -5.264114721663198 2216 88-89 -5.435432050344868 2216 90-91 -5.4303620294311195 2216 92-93 -5.11763557354211 2216 94-95 -8.182393474435923 2216 96-97 -10.217783531987905 2216 98-99 -7.167417391086005 2216 100-101 -6.228441468346318 2216 102-103 -7.03569731054834 2216 104-105 -9.728098244175296 2216 106-107 -6.542170801978685 2216 108-109 -8.625792000750806 2216 110-111 -6.213621490940589 2216 112-113 -5.798718114578673 2216 114-115 -6.226477025916555 2216 116-117 -5.728250865988471 2216 118-119 -6.011483993916084 2216 120-121 -6.513111449159787 2216 122-123 -9.218510153312579 2216 124-125 -7.966688585177252 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 197785.0 3 29431.0 4 16669.0 5 13381.0 6 13222.0 7 14643.0 8 16334.0 9 17147.0 10 17981.0 11 18710.0 12 18801.0 13 18999.0 14 19187.0 15 16689.0 16 14296.0 17 12722.0 18 11719.0 19 10974.0 20 10984.0 21 12139.0 22 15268.0 23 20574.0 24 27580.0 25 35317.0 26 42682.0 27 48447.0 28 55288.0 29 62815.0 30 69922.0 31 79182.0 32 93412.0 33 112871.0 34 138983.0 35 172846.0 36 224631.0 37 240158.0 38 72368.0 39 312.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.26243601675776 19.02127813933427 13.96199938283905 23.754286461068922 2 14.758048217049721 20.34061503578613 41.049147146470126 23.85218960069402 3 18.615328763320306 25.57200899302281 29.162066607368736 26.65059563628815 4 11.130255030783688 16.676533870003237 38.810641083458655 33.38257001575442 5 13.45258760917776 38.15962986969446 34.24743399665731 14.14034852447047 6 31.145040972152238 37.03781292552634 17.934519019646874 13.88262708267454 7 27.876018772518734 32.5044652741167 21.470249211441033 18.149266741923537 8 24.836343364453782 36.75956138482778 20.098429779922967 18.305665470795475 9 25.402598253632803 17.192021458006614 20.76036325311403 36.64501703524655 10-11 24.07800964875789 27.460141440607984 27.7902605909709 20.671588319663226 12-13 24.820890776573112 24.595978789119137 28.368051427177143 22.215079007130612 14-15 22.134386566854076 26.0247591959554 25.944282252152984 25.896571985037543 16-17 22.04996884394915 29.500708769124973 26.19893697044023 22.250385416485646 18-19 21.993553869478145 28.262084384580227 28.129343647075967 21.61501809886566 20-21 22.737023675338055 27.487724888371357 28.18069859086317 21.59455284542742 22-23 22.39157552941527 27.341324366693847 27.983610810113042 22.28348929377784 24-25 22.157941667403115 27.510110844445418 28.071159691267983 22.260787796883484 26-27 22.089809944390982 27.79038604122509 27.735405021467418 22.384398992916505 28-29 22.101112476019303 27.730907839904127 27.905750676200448 22.26222900787612 30-31 22.15536015416776 27.51310669177221 28.069120331369334 22.262412822690695 32-33 22.043435394480003 27.631273245735095 27.849755851521184 22.475535508263718 34-35 22.317400312493948 27.499293735871618 27.9782872809402 22.205018670694233 36-37 22.362183108520004 27.337530753391064 27.85487775092682 22.44540838716211 38-39 22.160240361535205 27.583615937852322 28.0123755212111 22.243768179401368 40-41 22.329319203516928 27.494645702682934 27.94161097187759 22.23442412192255 42-43 22.15883575883576 27.466832159369474 27.830179663015485 22.544152418779284 44-45 22.242004112866272 27.588854510088844 27.773575679025477 22.395565698019404 46-47 22.3184737280136 27.756722416145564 27.45221464372615 22.472589212114684 48-49 22.34894322625967 27.656306821214166 27.456085152543046 22.53866479998312 50-51 22.34509759531654 27.824296656399746 27.295038913017144 22.535566835266575 52-53 22.560571619642772 27.750431745702343 27.172313265794745 22.516683368860143 54-55 22.42597221163797 27.707414209830354 27.297412623640287 22.569200954891386 56-57 22.262690197830565 27.904683893067244 27.342219574552583 22.490406334549608 58-59 22.40486793381163 27.63588105317327 27.36182146358247 22.59742954943263 60-61 22.497389991719974 27.55386672261167 27.528989402311204 22.419753883357146 62-63 22.455728735507787 27.622325113580825 27.45755441863721 22.46439173227418 64-65 22.248961252786604 27.522488296951014 27.460656184911524 22.767894265350858 66-67 22.184894475198327 27.853543395822694 27.246381416375353 22.715180712603626 68-69 22.19751289399988 27.696545068308787 27.436679278104798 22.669262759586537 70-71 22.068760442007658 27.475433745888044 27.669312864702363 22.786492947401943 72-73 22.26638910613811 27.56370145679565 27.36476432465249 22.80514511241375 74-75 22.23235733443736 27.711547922037244 27.351002681130176 22.705092062395227 76-77 22.330718371488743 27.516300899664696 27.442181078743744 22.710799650102814 78-79 22.215817193529926 27.580227328867192 27.45192814948046 22.75202732812242 80-81 22.18892605164037 27.7892685832718 27.325163043815504 22.696642321272332 82-83 22.27518596329583 27.724590642461983 27.33953343367872 22.66068996056347 84-85 22.31253796078215 27.67603191895213 27.21661802970541 22.79481209056031 86-87 22.220741218412734 27.58104834716252 27.44453123623194 22.75367919819281 88-89 22.29635423247058 27.45201366495735 27.40400809293521 22.847624009636856 90-91 22.189013653379984 27.7129409498638 27.4218578187853 22.676187577970914 92-93 22.239632768473765 27.61124271752124 27.347643792965137 22.80148072103986 94-95 22.226902426510794 27.491040111557098 27.34610822259493 22.93594923933718 96-97 22.27853781768739 27.4909515840871 27.355032984336596 22.875477613888915 98-99 21.95763367522891 27.407998335986306 27.43552505415367 23.198842934631116 100-101 21.99964803581397 27.447845137743844 27.472517380403538 23.079989446038653 102-103 22.070659309838145 27.258960860381592 27.562467267821816 23.107912561958447 104-105 22.104225876512565 27.244480297859138 27.53908780639156 23.112206019236737 106-107 21.903813753260202 27.330740064514014 27.665894245862138 23.099551936363646 108-109 21.900800497211254 27.33187535664458 27.382931843685242 23.384392302458927 110-111 21.778099680849323 27.37383520427083 27.58484002007537 23.263225094804483 112-113 21.899377248071414 27.38269196925781 27.562277054486884 23.155653728183886 114-115 21.80747937048487 27.462293503176493 27.47964351800716 23.250583608331482 116-117 21.53325274468872 27.326798537489854 27.73310927373414 23.406839444087286 118-119 21.75640835024711 27.365097063195766 27.416552012067214 23.461942574489914 120-121 21.70777446951635 27.376841039210554 27.532799571070598 23.3825849202025 122-123 21.675036171601203 27.350868627276252 27.596952178028072 23.377143023094472 124-125 21.681654819188545 27.362136479769223 27.325978161965423 23.63023053907681 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 421.0 1 507.0 2 680.5 3 747.5 4 669.5 5 546.0 6 469.5 7 543.0 8 733.0 9 844.5 10 822.0 11 816.0 12 884.0 13 982.0 14 1102.5 15 1219.5 16 1311.5 17 1473.0 18 1718.5 19 2033.0 20 2523.5 21 3147.0 22 3996.5 23 5212.0 24 6637.0 25 8720.0 26 11551.0 27 14727.5 28 17925.5 29 21329.0 30 25470.5 31 29841.0 32 33978.0 33 38250.0 34 42596.0 35 46544.0 36 51021.0 37 55555.0 38 58982.5 39 61079.5 40 62568.5 41 65081.0 42 67554.5 43 69837.0 44 72196.0 45 73888.5 46 74638.5 47 74653.0 48 75333.0 49 74975.5 50 72861.5 51 70855.5 52 69179.5 53 67769.0 54 65046.5 55 59935.5 56 54706.0 57 50041.0 58 45670.5 59 40694.0 60 34342.0 61 27756.5 62 21780.5 63 16724.5 64 12529.5 65 9364.0 66 6935.0 67 5221.5 68 4019.0 69 2954.0 70 2125.5 71 1579.0 72 1172.0 73 870.5 74 653.5 75 492.0 76 343.5 77 209.0 78 136.5 79 96.0 80 66.5 81 45.0 82 35.5 83 25.5 84 17.5 85 15.5 86 10.0 87 9.5 88 8.5 89 7.0 90 6.0 91 5.5 92 4.5 93 5.5 94 5.5 95 3.0 96 1.5 97 1.5 98 2.0 99 1.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10067169065396389 2 0.03474861117247274 3 0.045073912778007506 4 0.0528675298552621 5 0.05579634136837052 6 0.0025813254013836895 7 0.0017374305586236372 8 0.0018863531779342347 9 0.005758341280009769 10-11 0.06237375705458858 12-13 0.006229929574493328 14-15 0.011293298631053642 16-17 0.02055132146486245 18-19 0.019484042693136503 20-21 0.13782788417195796 22-23 0.016704153799338685 24-25 0.014644057565542086 26-27 0.006825620051735718 28-29 0.004393217269662626 30-31 0.0019856349241413 32-33 0.006006545645527431 34-35 0.01737430558623637 36-37 0.002829529766901352 38-39 0.008438948427600524 40-41 0.03306082148695264 42-43 0.013973905778644397 44-45 0.1115430418636375 46-47 0.0034996815537990406 48-49 0.010002635930361797 50-51 0.01156632343312307 52-53 0.01303072918967728 54-55 0.010350122042086525 56-57 0.005907263899320367 58-59 0.015314209352439774 60-61 0.029114372075221804 62-63 0.007669514894495771 64-65 0.007123465290356913 66-67 0.008910536722084082 68-69 0.0079425396965652 70-71 0.0034500406806955083 72-73 6.701517868976886E-4 74-75 9.9281746207065E-4 76-77 0.008091462315875797 78-79 0.021171832378656608 80-81 0.0028047093303495856 82-83 0.004616601198628522 84-85 0.002705427584142521 86-87 0.0034500406806955083 88-89 0.005932084335872134 90-91 0.004765523817939119 92-93 0.0022338392896589622 94-95 0.0036982450462131706 96-97 0.0013899444468989098 98-99 0.003102554568970781 100-101 0.0029536319496601834 102-103 6.949722234494549E-4 104-105 0.0046662420717320545 106-107 0.0011417400813812475 108-109 0.0016629692489683386 110-111 0.00297845238621195 112-113 0.005137830366215613 114-115 0.0030529136958672484 116-117 0.006155468264838029 118-119 0.004244294650352028 120-121 0.007520592275185173 122-123 0.005584598224147405 124-125 0.05465460128698927 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2014469.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.28829105737866 #Duplication Level Percentage of deduplicated Percentage of total 1 85.38118045247823 61.720596233713145 2 8.929231106678571 12.909577143163595 3 2.592765937647782 5.622798562330203 4 1.0715357774028844 3.0983796062117683 5 0.5578440715282633 2.016279730363413 6 0.3294665890027939 1.4289946007709426 7 0.21857009642991315 1.1060041123015416 8 0.1609544253943443 0.9308096279903598 9 0.12449106781421242 0.8099321889777885 >10 0.5945773871863582 7.610533818029666 >50 0.02941408760568274 1.4542840307471514 >100 0.00975708804149406 1.1001719897589157 >500 7.063759647436553E-5 0.042739489387667855 >1k 1.4127519294873105E-4 0.14889886625381057 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.964087310353249E-5 0.0 7 0.0 0.0 0.0 4.964087310353249E-5 0.0 8 0.0 0.0 0.0 9.928174620706498E-5 0.0 9 0.0 0.0 0.0 9.928174620706498E-5 0.0 10-11 0.0 0.0 0.0 9.928174620706498E-5 0.0 12-13 7.446130965529873E-5 0.0 0.0 9.928174620706498E-5 0.0 14-15 9.928174620706498E-5 0.0 0.0 9.928174620706498E-5 0.0 16-17 1.2410218275883122E-4 0.0 0.0 1.4892261931059747E-4 0.0 18-19 1.9856349241413E-4 0.0 0.0 1.7374305586236372E-4 0.0 20-21 3.4748611172472744E-4 0.0 0.0 2.4820436551766244E-4 0.0 22-23 3.4748611172472744E-4 0.0 0.0 2.9784523862119494E-4 2.4820436551766246E-5 24-25 5.460496041388575E-4 0.0 0.0 4.4676785793179244E-4 4.964087310353249E-5 26-27 5.460496041388575E-4 0.0 0.0 6.701517868976886E-4 4.964087310353249E-5 28-29 6.949722234494549E-4 0.0 0.0 9.431765889671175E-4 4.964087310353249E-5 30-31 7.446130965529874E-4 0.0 0.0 0.0024820436551766245 4.964087310353249E-5 32-33 7.694335331047536E-4 0.0 0.0 0.005807982153113301 4.964087310353249E-5 34-35 8.687152793118186E-4 2.4820436551766246E-5 0.0 0.009952995057258265 4.964087310353249E-5 36-37 9.9281746207065E-4 4.964087310353249E-5 0.0 0.01725020340347754 4.964087310353249E-5 38-39 0.0011913809544847798 4.964087310353249E-5 0.0 0.031497133984191364 4.964087310353249E-5 40-41 0.0011913809544847798 4.964087310353249E-5 0.0 0.04780416079870179 4.964087310353249E-5 42-43 0.0013403035737953773 4.964087310353249E-5 0.0 0.06587343860838762 4.964087310353249E-5 44-45 0.0013899444468989098 4.964087310353249E-5 0.0 0.08595317177876652 4.964087310353249E-5 46-47 0.0016381488124165722 4.964087310353249E-5 0.0 0.11223801408708697 7.446130965529873E-5 48-49 0.0016381488124165722 4.964087310353249E-5 0.0 0.1397142373498922 9.928174620706498E-5 50-51 0.0016381488124165722 4.964087310353249E-5 0.0 0.17349485149684607 9.928174620706498E-5 52-53 0.0016381488124165722 4.964087310353249E-5 0.0 0.20672941603966108 1.2410218275883122E-4 54-55 0.0016877896855201047 4.964087310353249E-5 0.0 0.24242120380110094 1.4892261931059747E-4 56-57 0.0018367123048307022 4.964087310353249E-5 0.0 0.28804116618324727 1.4892261931059747E-4 58-59 0.0018367123048307022 4.964087310353249E-5 0.0 0.34088387560195765 1.4892261931059747E-4 60-61 0.001911173614486001 4.964087310353249E-5 0.0 0.4018428677730955 1.9856349241412997E-4 62-63 0.0019359940510377672 4.964087310353249E-5 0.0 0.46645046411734303 1.9856349241412997E-4 64-65 0.0019359940510377672 4.964087310353249E-5 0.0 0.5322742618526272 1.9856349241412997E-4 66-67 0.0019359940510377672 4.964087310353249E-5 0.0 0.6090190516706884 1.9856349241412997E-4 68-69 0.0019359940510377672 4.964087310353249E-5 0.0 0.697305344485321 1.9856349241412997E-4 70-71 0.0019359940510377672 4.964087310353249E-5 0.0 0.8002356948654956 1.9856349241412997E-4 72-73 0.001960814487589534 4.964087310353249E-5 0.0 0.9081301325560234 1.9856349241412997E-4 74-75 0.0019856349241413 4.964087310353249E-5 0.0 1.0173896942568985 1.9856349241412997E-4 76-77 0.0019856349241413 4.964087310353249E-5 0.0 1.1420627470564204 1.9856349241412997E-4 78-79 0.002010455360693066 4.964087310353249E-5 0.0 1.289372037991153 1.9856349241412997E-4 80-81 0.0022586597262107284 4.964087310353249E-5 0.0 1.453633687090742 2.2338392896589624E-4 82-83 0.0023331210358660272 4.964087310353249E-5 0.0 1.634897335228291 2.9784523862119494E-4 84-85 0.0024324027820730922 4.964087310353249E-5 0.0 1.8167318534065304 2.9784523862119494E-4 86-87 0.0025316845282801572 4.964087310353249E-5 0.0 2.0051934281440915 3.226656751729612E-4 88-89 0.0025316845282801572 4.964087310353249E-5 0.0 2.2194682568954898 3.4748611172472744E-4 90-91 0.0027302480206942872 4.964087310353249E-5 0.0 2.4698568208297074 3.4748611172472744E-4 92-93 0.002829529766901352 4.964087310353249E-5 0.0 2.7409704492846503 3.4748611172472744E-4 94-95 0.002829529766901352 4.964087310353249E-5 0.0 3.0137222265520096 3.4748611172472744E-4 96-97 0.002829529766901352 7.446130965529873E-5 0.0 3.283991960164192 3.4748611172472744E-4 98-99 0.0029288115131084168 9.928174620706498E-5 0.0 3.5772205975867584 3.4748611172472744E-4 100-101 0.0030529136958672484 9.928174620706498E-5 0.0 3.920760259899755 3.4748611172472744E-4 102-103 0.0031521954420743134 9.928174620706498E-5 0.0 4.287184364713481 3.4748611172472744E-4 104-105 0.0038719881020755345 9.928174620706498E-5 0.0 4.656835126278935 3.4748611172472744E-4 106-107 0.003996090284834366 9.928174620706498E-5 0.0 5.0265107082809415 3.4748611172472744E-4 108-109 0.0040705515944896645 9.928174620706498E-5 0.0 5.412667060153321 3.4748611172472744E-4 110-111 0.00416983334069673 9.928174620706498E-5 0.0 5.853403552003034 3.723065482764937E-4 112-113 0.00416983334069673 9.928174620706498E-5 0.0 6.337476526072131 3.9712698482825994E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4440 0.0 50.41048 1 GGTATCA 2715 0.0 49.551243 1 ATCAACG 5210 0.0 41.00255 3 TCAACGC 5330 0.0 39.574284 4 CAACGCA 5420 0.0 39.02708 5 TATCAAC 5835 0.0 38.038387 2 AACGCAG 5575 0.0 36.744423 6 ACGCAGA 6520 0.0 31.053402 7 GTACATG 6895 0.0 30.993895 1 CGCAGAG 6330 0.0 30.669968 8 TACATGG 6880 0.0 30.098503 2 ACATGGG 6965 0.0 27.937063 3 GCAGAGT 7125 0.0 25.158287 9 CGCATAG 245 1.607259E-8 24.307112 8 AACGCAT 320 4.965841E-10 22.331049 6 AGAGTAC 6945 0.0 21.595772 10-11 CAGAGTA 7315 0.0 21.317064 10-11 CATGGGG 3435 0.0 21.162651 4 GTGTAGC 1280 0.0 20.4625 1 CATGGGA 4050 0.0 20.30304 4 >>END_MODULE