FastQCFastQC Report
Thu 26 May 2016
SRR1364490_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364490_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014469
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46650.23157467302797907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42400.21047730195897776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27410.13606563317678258No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38950.060.341531
GTATCAA63700.051.692461
TATCAAC85750.037.589042
ATCAACG80650.037.5281753
CAACGCA81200.037.2898675
TCAACGC81150.037.076694
AACGCAG84750.035.657686
GTACATG71250.029.2191871
CGCAGAG103250.029.2037898
ACGCAGA101900.029.1310027
TACATGG73750.027.790382
GCAGAGT109000.026.4628439
ACATGGG72900.025.9077573
GAGTACT69300.025.18876812-13
GTGGTAT19200.024.8551461
AGAGTAC103250.024.509910-11
TGGTATC18750.023.514112
AGTACTT75200.023.4893312-13
CAGAGTA109550.023.37183410-11
GTACTTT76750.022.97649214-15
Produced by FastQC (version 0.11.5)