##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364490_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2014469 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.006377859376343 31.0 28.0 33.0 25.0 34.0 2 29.16306828251018 31.0 28.0 33.0 25.0 34.0 3 29.241296341616575 31.0 28.0 34.0 25.0 34.0 4 32.66265750428525 35.0 32.0 37.0 26.0 37.0 5 31.823384226811136 35.0 32.0 37.0 22.0 37.0 6 31.886522453311517 35.0 32.0 37.0 22.0 37.0 7 31.326808702442182 35.0 31.0 37.0 17.0 37.0 8 31.45532098036753 35.0 32.0 37.0 17.0 37.0 9 32.04939862564279 37.0 30.0 39.0 13.0 39.0 10-11 32.22926215295445 37.0 31.0 39.0 17.0 39.0 12-13 32.00147036266132 37.0 30.5 39.0 13.5 39.0 14-15 32.93555597033263 38.0 31.0 40.0 14.5 41.0 16-17 32.98395904826532 38.0 31.0 40.0 16.0 41.0 18-19 32.861682408614875 38.0 31.0 40.0 11.5 41.0 20-21 32.76591250597552 38.0 31.0 40.0 11.5 41.0 22-23 32.73348013794206 38.0 31.0 40.0 10.5 41.0 24-25 32.590408191935445 38.0 31.0 40.0 10.0 41.0 26-27 32.62116319486674 37.0 31.0 40.0 10.0 41.0 28-29 32.488046725961034 37.0 30.5 40.0 10.0 41.0 30-31 32.003827311316286 37.0 30.0 40.0 9.0 41.0 32-33 31.9449085590297 37.0 30.0 40.0 8.0 41.0 34-35 31.79530387412266 37.0 30.0 40.0 8.0 41.0 36-37 31.744488746165864 37.0 30.0 40.0 7.0 41.0 38-39 31.537859356485505 37.0 30.0 40.0 2.0 41.0 40-41 31.230642417431092 36.5 29.5 40.0 2.0 41.0 42-43 31.138652915482936 36.0 29.5 40.0 2.0 41.0 44-45 31.063608325568673 36.0 29.0 40.0 2.0 41.0 46-47 30.911872806183666 36.0 29.0 40.0 2.0 41.0 48-49 30.640705069177038 36.0 28.0 40.0 2.0 41.0 50-51 30.33836038181774 36.0 28.0 40.0 2.0 41.0 52-53 30.229510853728698 36.0 28.0 40.0 2.0 41.0 54-55 29.769225786050814 35.0 26.5 40.0 2.0 41.0 56-57 29.790276246494734 35.0 27.0 39.5 2.0 41.0 58-59 29.580585752374446 35.0 26.5 39.0 2.0 41.0 60-61 29.317428066651807 35.0 26.0 39.0 2.0 41.0 62-63 29.067665225923058 34.5 26.0 39.0 2.0 41.0 64-65 28.59327222210915 34.0 25.5 38.5 2.0 40.0 66-67 28.241727720803844 34.0 24.5 38.0 2.0 40.0 68-69 28.052401650261185 34.0 24.5 38.0 2.0 40.0 70-71 28.116309806703406 34.0 25.5 37.0 2.0 40.0 72-73 27.905249969098556 34.0 25.5 37.0 2.0 39.5 74-75 27.54577980599354 34.0 24.5 36.0 2.0 39.0 76-77 27.430289570105074 34.0 25.0 36.0 2.0 39.0 78-79 27.030064994795154 33.0 24.0 35.5 2.0 38.5 80-81 26.807560453896286 33.0 24.0 35.0 2.0 37.0 82-83 26.647590506480864 33.0 24.0 35.0 2.0 37.0 84-85 26.238398555649155 33.0 22.0 35.0 2.0 36.0 86-87 26.10401897472733 33.0 22.5 35.0 2.0 36.0 88-89 26.03002106262246 33.0 22.5 35.0 2.0 36.0 90-91 25.85570291724519 33.0 21.5 35.0 2.0 35.5 92-93 25.872441323246967 33.0 23.0 35.0 2.0 35.0 94-95 25.857745390969036 33.0 23.0 35.0 2.0 35.0 96-97 25.730868779812447 33.0 21.5 35.0 2.0 35.0 98-99 25.618367917302276 33.0 20.0 35.0 2.0 35.0 100-101 25.536299640252594 33.0 20.0 35.0 2.0 35.0 102-103 24.382611745328422 30.5 18.5 34.0 2.0 35.0 104-105 25.3124463568315 32.5 20.0 35.0 2.0 35.0 106-107 25.44340493698339 33.0 20.0 35.0 2.0 35.0 108-109 25.507067619308117 33.0 20.0 35.0 2.0 35.0 110-111 25.44627963994482 33.0 19.0 35.0 2.0 35.0 112-113 25.376625056032136 33.0 19.0 35.0 2.0 35.0 114-115 25.321668141827946 33.0 19.0 35.0 2.0 35.0 116-117 25.235206895712963 33.0 18.5 35.0 2.0 35.0 118-119 25.116599957606695 33.0 17.5 35.0 2.0 35.0 120-121 24.956021909495753 33.0 8.5 35.0 2.0 35.0 122-123 24.666344331930645 33.0 2.0 35.0 2.0 35.0 124-125 23.899252358810188 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5432403988819914 1101 2 0.5436703414813095 1101 3 0.5806595646917252 1101 4 0.6768199855892689 1101 5 0.7612565096887245 1101 6 0.8761401568720046 1101 7 1.2858334600472041 1101 8 1.2495849516419675 1101 9 1.856727081020825 1101 10-11 1.5753540385926073 1101 12-13 1.6894057924127708 1101 14-15 1.5341382539696866 1101 16-17 1.5013475805138299 1101 18-19 1.7050984968320293 1101 20-21 1.8510956166801265 1101 22-23 1.796651649464792 1101 24-25 1.9410786748578985 1101 26-27 1.559719030327507 1101 28-29 1.6235028155708164 1101 30-31 2.095012290519854 1101 32-33 1.890442696024845 1101 34-35 2.0271910698512414 1101 36-37 1.7920258473210104 1101 38-39 1.9167376882960667 1101 40-41 2.043045851340967 1101 42-43 2.0510813644405985 1101 44-45 1.896937739032488 1101 46-47 1.7372276126615418 1101 48-49 1.7524216342487016 1101 50-51 2.116324020709257 1101 52-53 1.8681092787673812 1101 54-55 2.2534280708882655 1101 56-57 1.9682835598953226 1101 58-59 1.9518443557210041 1101 60-61 1.9246831522250005 1101 62-63 2.131845390364486 1101 64-65 2.300703985884091 1101 66-67 2.445632070865038 1101 68-69 2.3687740091345866 1101 70-71 2.059945788489209 1101 72-73 2.2046892327126137 1101 74-75 2.2609923064990234 1101 76-77 2.052675350136056 1101 78-79 2.209063127268138 1101 80-81 2.1413030449877866 1101 82-83 1.9971141425523768 1101 84-85 2.3143624643205527 1101 86-87 2.205252514958019 1101 88-89 1.9373817686153956 1101 90-91 1.9893511962040051 1101 92-93 1.819679858230586 1101 94-95 1.8816769970979976 1101 96-97 1.771430618834092 1101 98-99 1.8630541340536126 1101 100-101 1.934502751899558 1101 102-103 1.6455582080988655 1101 104-105 1.8667767775166766 1101 106-107 2.0339917480313368 1101 108-109 1.9629500005914622 1101 110-111 1.9307358611860117 1101 112-113 1.7575896144591816 1101 114-115 1.8509523058614086 1101 116-117 1.892875886128678 1101 118-119 1.8730736544563058 1101 120-121 1.7730670567392472 1101 122-123 1.9118098693390415 1101 124-125 1.8116796341473922 1102 1 0.43403305734249287 1102 2 -1.8661085329618743 1102 3 -0.15197127129600219 1102 4 0.2537264909409984 1102 5 0.807085239263035 1102 6 0.3746564619254116 1102 7 1.2400034557366375 1102 8 0.9312247684407957 1102 9 1.541103756563576 1102 10-11 1.423093656650245 1102 12-13 1.5034327058213108 1102 14-15 1.4908175189528379 1102 16-17 1.4242487370722259 1102 18-19 1.5446917167256018 1102 20-21 1.6277950656410667 1102 22-23 1.61872621800579 1102 24-25 1.8488679311453922 1102 26-27 1.5294627240111325 1102 28-29 1.7134203087568665 1102 30-31 1.9536828700384845 1102 32-33 1.6867200837333307 1102 34-35 1.8576004033524427 1102 36-37 1.1809721483697864 1102 38-39 1.2015606391967566 1102 40-41 1.3196781805351243 1102 42-43 1.25782176773577 1102 44-45 1.0503835276372833 1102 46-47 0.8043856215156637 1102 48-49 0.7430209936643202 1102 50-51 0.9664437184437986 1102 52-53 0.7692444117929718 1102 54-55 1.203466254141997 1102 56-57 0.9083046695277339 1102 58-59 0.852499550545808 1102 60-61 0.7457516596892688 1102 62-63 1.0631167593566992 1102 64-65 1.1708323361361153 1102 66-67 1.3912130897676107 1102 68-69 1.316644797553078 1102 70-71 0.7771938427514016 1102 72-73 1.1123564084893296 1102 74-75 1.274797651134758 1102 76-77 1.166886472739403 1102 78-79 1.0602610067345672 1102 80-81 1.1797626223166233 1102 82-83 0.716370936014119 1102 84-85 0.6886776367805965 1102 86-87 0.7533179654086268 1102 88-89 0.9507381119187315 1102 90-91 0.6631439817459039 1102 92-93 0.7609524156692196 1102 94-95 0.7602942573406999 1102 96-97 0.8397152003695574 1102 98-99 0.6579757416960703 1102 100-101 0.4155008468552488 1102 102-103 0.22619351311579194 1102 104-105 0.5955199620902505 1102 106-107 0.8423189724362992 1102 108-109 0.6863709651785044 1102 110-111 0.7071517793863826 1102 112-113 0.7204534585067712 1102 114-115 0.9579982830487275 1102 116-117 0.608727908076105 1102 118-119 0.16030835732705384 1102 120-121 0.45598352189952607 1102 122-123 0.7540447349838004 1102 124-125 0.7403652986987801 1103 1 0.652061386370459 1103 2 0.5579454578346663 1103 3 0.4239202499450734 1103 4 0.44735795753310725 1103 5 0.7903444755126294 1103 6 0.8980972815186163 1103 7 1.1159355435111458 1103 8 0.9560416299812893 1103 9 1.8130770337032907 1103 10-11 1.4459019740855297 1103 12-13 1.4022102217514316 1103 14-15 1.166790222349043 1103 16-17 1.2358122804430778 1103 18-19 1.2799324003639825 1103 20-21 1.5209987575490374 1103 22-23 1.4386624503997965 1103 24-25 1.6823333282458037 1103 26-27 1.0899190327157697 1103 28-29 1.260287417819967 1103 30-31 1.9974549646617916 1103 32-33 1.8005336068798208 1103 34-35 1.7952392740241407 1103 36-37 1.677470781360082 1103 38-39 1.7499378011416553 1103 40-41 1.8569495176284967 1103 42-43 1.8576860779829687 1103 44-45 1.8194213271639939 1103 46-47 1.7499677080727878 1103 48-49 1.7145842169797412 1103 50-51 1.9128588326780545 1103 52-53 1.659966446999853 1103 54-55 1.8598326178490474 1103 56-57 1.743366514837689 1103 58-59 1.9015851541781856 1103 60-61 1.7779284704764322 1103 62-63 1.895640054678708 1103 64-65 2.0130594957640504 1103 66-67 2.2291838559449424 1103 68-69 2.3853590269421012 1103 70-71 2.063432657501753 1103 72-73 2.017964113224661 1103 74-75 2.1169656594161417 1103 76-77 2.0220148601234875 1103 78-79 2.1728788381804236 1103 80-81 2.0454523967227125 1103 82-83 2.049610478056046 1103 84-85 2.2185982041308634 1103 86-87 2.1133320576646994 1103 88-89 1.9998767308658572 1103 90-91 2.1632632662040905 1103 92-93 1.8422861062341447 1103 94-95 1.7329368822820896 1103 96-97 1.6820053402278852 1103 98-99 1.906619285962286 1103 100-101 1.903028012295234 1103 102-103 1.7833673393370866 1103 104-105 1.7473123313407406 1103 106-107 1.8003843984398777 1103 108-109 1.8253285721692976 1103 110-111 1.770959343218788 1103 112-113 1.7641297328574908 1103 114-115 1.5696501558245366 1103 116-117 1.703660812286465 1103 118-119 1.7451142564785727 1103 120-121 1.6241612559385814 1103 122-123 1.7826042813926222 1103 124-125 1.5950713307420195 1104 1 0.6727748609614643 1104 2 0.548346250795607 1104 3 0.5233549444484389 1104 4 0.5589377576215924 1104 5 0.9212980244754299 1104 6 0.9009460989040647 1104 7 1.3869858847425043 1104 8 1.212773366997002 1104 9 1.8235277336621856 1104 10-11 1.3291736262541178 1104 12-13 1.4034509485924929 1104 14-15 1.307441790700139 1104 16-17 1.1916383572785136 1104 18-19 1.1117313521603407 1104 20-21 1.5235152781904233 1104 22-23 1.5467539274126523 1104 24-25 1.7471982029411137 1104 26-27 1.5511506440127079 1104 28-29 1.5270686365534871 1104 30-31 2.075192910767605 1104 32-33 1.9125042263637617 1104 34-35 1.8724341485776819 1104 36-37 1.7949495714455246 1104 38-39 1.986524782495735 1104 40-41 2.167352168392174 1104 42-43 2.0181083233427515 1104 44-45 1.7386095195965439 1104 46-47 1.7758158341914836 1104 48-49 1.516901648690574 1104 50-51 1.855623539723453 1104 52-53 1.8726899429070905 1104 54-55 2.2024930939538976 1104 56-57 2.0198115253689686 1104 58-59 2.191732066084761 1104 60-61 2.1776341225241573 1104 62-63 2.2648835242036576 1104 64-65 2.4039365745072807 1104 66-67 2.2756762087364564 1104 68-69 2.453549247289441 1104 70-71 2.1901022551195872 1104 72-73 2.376833311974483 1104 74-75 2.431251323073269 1104 76-77 2.211267135558785 1104 78-79 2.1878466940895436 1104 80-81 2.1546630786407697 1104 82-83 2.078761380134921 1104 84-85 2.4876032836172115 1104 86-87 2.3138704380785384 1104 88-89 2.1211639232931816 1104 90-91 2.2306892879769435 1104 92-93 2.003386934560485 1104 94-95 1.956568487460956 1104 96-97 1.9722697637531859 1104 98-99 1.9542163878646335 1104 100-101 1.9501409383494597 1104 102-103 1.8584502223335697 1104 104-105 1.734482580681739 1104 106-107 1.9785190022168173 1104 108-109 1.9337157401243665 1104 110-111 1.8487469053413967 1104 112-113 1.8186287304647308 1104 114-115 1.736970053414094 1104 116-117 1.814916755949529 1104 118-119 1.7966839039362554 1104 120-121 1.7065214189775588 1104 122-123 1.7944968302606696 1104 124-125 1.4549312643535544 1105 1 0.4525254377765755 1105 2 0.4678027287373432 1105 3 0.4007942986008608 1105 4 0.6221881267274725 1105 5 0.8336624118126288 1105 6 0.820929580511752 1105 7 1.2674422946929162 1105 8 1.0242817539960392 1105 9 1.6766585354425168 1105 10-11 1.3689478875009868 1105 12-13 1.5338827483322461 1105 14-15 1.4996886712554627 1105 16-17 1.4500426851200032 1105 18-19 1.5158337064284169 1105 20-21 1.8230688711913459 1105 22-23 1.7226903612952569 1105 24-25 1.9178276730277233 1105 26-27 1.6400882573887117 1105 28-29 1.7469070384869667 1105 30-31 2.056418322424797 1105 32-33 1.9284079544347925 1105 34-35 2.0712825231075556 1105 36-37 1.6829551457882665 1105 38-39 1.6817134934020288 1105 40-41 1.8853953251980293 1105 42-43 1.9051501987155035 1105 44-45 1.7648451389355486 1105 46-47 1.6149076573697911 1105 48-49 1.7290719723415542 1105 50-51 1.7714087227028514 1105 52-53 1.640643836280244 1105 54-55 1.8604259175824218 1105 56-57 1.766703144648961 1105 58-59 1.7759877346366153 1105 60-61 1.6607859864840187 1105 62-63 1.5349032568171452 1105 64-65 1.8308042483596232 1105 66-67 2.123916127131743 1105 68-69 2.0625718658934 1105 70-71 1.9303782904293065 1105 72-73 2.122045526695807 1105 74-75 2.114726093801142 1105 76-77 1.886713540193771 1105 78-79 1.9869640646050826 1105 80-81 1.921048727119583 1105 82-83 1.767383160109901 1105 84-85 2.0449797320853236 1105 86-87 1.9088144085958376 1105 88-89 1.7657201872466501 1105 90-91 1.8480802292822744 1105 92-93 1.6635297598353667 1105 94-95 1.5711559924338232 1105 96-97 1.5940695971129841 1105 98-99 1.7549981745876764 1105 100-101 1.6943197606319664 1105 102-103 1.5460201226605612 1105 104-105 1.140212187611521 1105 106-107 1.4117188052594791 1105 108-109 1.4509366463872198 1105 110-111 1.6080561607948134 1105 112-113 1.5633739422066313 1105 114-115 1.4727286375075863 1105 116-117 1.330319769071476 1105 118-119 1.4264146554654218 1105 120-121 1.5271521798590761 1105 122-123 1.6203027803807615 1105 124-125 1.4304140571117507 1106 1 0.4297923340623555 1106 2 0.3426226706705471 1106 3 0.29778311722674644 1106 4 0.23554945921898707 1106 5 0.17428300937357832 1106 6 0.5252496024278344 1106 7 0.6999850468920599 1106 8 0.5721991274356988 1106 9 1.2588498266597803 1106 10-11 0.8991876931619203 1106 12-13 0.7132628926414384 1106 14-15 0.9515737555697754 1106 16-17 0.9403639361448981 1106 18-19 0.9156848590705167 1106 20-21 0.8379074332924432 1106 22-23 0.6882379310136599 1106 24-25 0.5804330514317257 1106 26-27 0.5498514906906422 1106 28-29 0.5061420660404963 1106 30-31 1.008189131689214 1106 32-33 0.8947746241410677 1106 34-35 0.8434046948242155 1106 36-37 0.6953890053599814 1106 38-39 0.960822188583812 1106 40-41 1.0820351074436836 1106 42-43 1.043625188031804 1106 44-45 0.9760297297516587 1106 46-47 1.100898144817112 1106 48-49 1.024189558759769 1106 50-51 1.2930548127767096 1106 52-53 0.9601489064990254 1106 54-55 1.5488291058466537 1106 56-57 1.2019850205294489 1106 58-59 1.1894946591443727 1106 60-61 1.4174878848988115 1106 62-63 1.4480281858178508 1106 64-65 1.66746298886504 1106 66-67 1.7424788580484858 1106 68-69 1.5291621109191986 1106 70-71 1.4118639935869695 1106 72-73 1.3636985698703334 1106 74-75 1.567620044236488 1106 76-77 1.1515035619630218 1106 78-79 1.0925684588719733 1106 80-81 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-5.492403424341891 2216 86-87 -5.947942030477904 2216 88-89 -5.457456656395447 2216 90-91 -4.956066891455464 2216 92-93 -4.520475805200942 2216 94-95 -4.238672985320953 2216 96-97 -4.442779707445201 2216 98-99 -4.379290142773421 2216 100-101 -4.734040649402463 2216 102-103 -4.727193095686925 2216 104-105 -4.581001808192944 2216 106-107 -4.599373049126566 2216 108-109 -4.669190950515009 2216 110-111 -4.327998194961836 2216 112-113 -4.3621480182094885 2216 114-115 -4.244844229590303 2216 116-117 -4.172079700378635 2216 118-119 -4.395250325074567 2216 120-121 -4.265767012267236 2216 122-123 -4.2292944312480145 2216 124-125 -4.081557009089249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96936.0 3 32124.0 4 15435.0 5 12341.0 6 12772.0 7 14825.0 8 16613.0 9 17776.0 10 18906.0 11 20396.0 12 22091.0 13 23320.0 14 23895.0 15 19884.0 16 14756.0 17 12906.0 18 11467.0 19 10673.0 20 10613.0 21 11394.0 22 13834.0 23 19210.0 24 26020.0 25 34703.0 26 42345.0 27 48152.0 28 54566.0 29 61852.0 30 69320.0 31 78890.0 32 92532.0 33 109680.0 34 132163.0 35 166672.0 36 232164.0 37 302320.0 38 110284.0 39 639.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.31155768905851 20.265660872417186 14.40989097269845 24.012890465825855 2 15.779276383555931 20.890112263057436 39.188816881901644 24.141794471484985 3 19.255595395114046 25.262935294611133 29.513583976720415 25.967885333554403 4 12.952637373895106 17.87558943439164 36.6482728987988 32.523500292914456 5 13.149271594648514 38.51069438149706 34.315147068532696 14.024886955321724 6 31.27806575042716 37.74444443341312 17.195425487449278 13.782064328710437 7 27.290866726391126 32.6975028951796 21.24058694179646 18.771043436632812 8 25.350749999131285 36.88048810877705 19.331496290089348 18.437265602002313 9 25.332333235209877 16.50946229502663 20.286487406855105 37.87171706290839 10-11 24.695029630798953 27.406875695596533 27.213672362491547 20.684422311112964 12-13 25.41452796282875 24.418901343288027 28.01398389642882 22.152586797454404 14-15 22.3762418014731 25.809217098853914 26.01075969396303 25.80378140570996 16-17 21.89131710397374 29.17002999443003 26.582005129136892 22.356647772459333 18-19 21.770358323975866 27.57357649841097 29.061984656732363 21.594080520880805 20-21 22.467917778766772 26.74071538618677 29.263408930077993 21.527957904968464 22-23 22.11472328393247 26.523718644178018 29.16981580723363 22.191742264655883 24-25 21.932976710463052 26.79472060473813 29.15298882845887 22.119313856339943 26-27 21.84395121055218 27.04475841081827 29.071545942403827 22.03974443622572 28-29 21.791596329118416 26.969953983668038 29.266228271685748 21.9722214155278 30-31 21.810571808114567 26.792199565936542 29.17304557269173 22.224183053257164 32-33 21.78603030329355 26.856576241612238 29.103770599426397 22.253622855667814 34-35 22.05335019157887 26.929136047183235 29.051060974874215 21.966452786363686 36-37 22.1691940803517 26.71784373256343 28.955334573708676 22.157627613376196 38-39 21.926771848003742 26.947081286152724 29.067850773719478 22.058296092124056 40-41 22.078321790796714 26.775971203807718 28.94586218553806 22.199844819857496 42-43 22.01955868847576 26.883715157983566 28.73258209436819 22.364144059172478 44-45 22.189252106219538 26.981650827359623 28.57233275277799 22.25676431364285 46-47 22.245870617821296 27.280902234140463 28.07711820403025 22.396108944007988 48-49 22.3377282891244 27.357886211673204 27.917537553950332 22.38684794525206 50-51 22.29094010684697 27.380213073172673 27.74527562818843 22.58357119179193 52-53 22.46854317580204 27.401037743224947 27.615560936078644 22.51485814489437 54-55 22.31752627130081 27.276968269144973 27.64617445968022 22.75933099987399 56-57 22.2248559482841 27.583120601349986 27.70461672873849 22.48740672162743 58-59 22.35926688372966 27.366194267571252 27.667216522071076 22.607322326628008 60-61 22.41340025584906 27.40479004640925 27.812788382447184 22.369021315294503 62-63 22.30705613765284 27.321355262132414 27.908932373406458 22.46265622680829 64-65 22.206109344323977 27.12395891671458 27.788339867203284 22.881591871758157 66-67 22.10422400085809 27.443342811372457 27.65731873126035 22.795114456509108 68-69 22.15646059767264 27.377712956589967 27.743697288141732 22.722129157595656 70-71 22.08770228047375 27.180990326676945 27.883927073291083 22.847380319558223 72-73 22.26040538899267 27.225028420048947 27.588844786172196 22.92572140478619 74-75 22.130997186963775 27.426346133272833 27.607866771347787 22.834789908415605 76-77 22.255018837783588 27.264784600946516 27.63416848045023 22.846028080819664 78-79 22.258799853259248 27.397456460539594 27.520791168946957 22.8229525172542 80-81 22.062085963270643 27.486330117425716 27.63378862377852 22.817795295525116 82-83 22.251309030473553 27.394428702764205 27.544170470814134 22.81009179594811 84-85 22.15599300758488 27.302561444867468 27.433265896190484 23.10817965135717 86-87 22.184279925786655 27.315251586961782 27.627816352997385 22.872652134254174 88-89 22.223945890602216 27.21764760634172 27.559206974651733 22.999199528404333 90-91 22.120058268615935 27.418590607375116 27.622019460764612 22.839331663244334 92-93 22.192908580904533 27.40425844554302 27.586072520608283 22.81676045294416 94-95 22.108123376674953 27.24711375785352 27.593199707702205 23.051563157769323 96-97 22.21007094103289 27.230375798089725 27.546198793250365 23.013354467627025 98-99 21.90934683035579 27.101831797858395 27.589876041775774 23.398945330010044 100-101 21.916853132186368 27.09636265030486 27.749370484959275 23.237413732549495 102-103 22.064116562390403 27.062675715663975 27.597017633432213 23.27619008851341 104-105 22.020368192142378 26.992632533360606 27.736477858011398 23.250521416485622 106-107 21.791683958246047 27.082733505818908 27.75812274009813 23.367459795836915 108-109 21.86898880052262 27.056087733293488 27.511046335287364 23.56387713089653 110-111 21.712751667191206 27.075140508700567 27.717643286111553 23.49446453799667 112-113 21.955871227539976 27.076007164174364 27.665393543109147 23.302728065176513 114-115 21.862289593795676 27.167738952223825 27.449500897632202 23.520470556348293 116-117 21.606165197875963 27.095676329593555 27.672081327635222 23.626077144895255 118-119 21.697819105869645 27.063206862025297 27.54385938785246 23.695114644252595 120-121 21.639064641186078 27.084312205578186 27.630370890505056 23.646252262730677 122-123 21.69020481915749 27.06156984998461 27.541624058060226 23.706601272797673 124-125 21.563390423888624 27.027780418598983 27.54185108631249 23.866978071199902 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 173.0 1 144.0 2 219.0 3 357.5 4 295.5 5 233.0 6 462.0 7 1071.5 8 1748.0 9 2004.0 10 1699.0 11 1240.0 12 1084.0 13 1167.0 14 1293.5 15 1483.5 16 1730.0 17 1924.5 18 2165.0 19 2571.5 20 3093.0 21 3714.5 22 4567.5 23 5721.0 24 7168.5 25 9072.5 26 11521.0 27 14635.0 28 17807.0 29 21099.0 30 25103.5 31 29452.0 32 33439.5 33 37407.0 34 41973.5 35 45941.5 36 49833.0 37 54556.5 38 58113.0 39 60218.0 40 61851.5 41 64213.0 42 66820.5 43 69045.5 44 71732.5 45 73578.0 46 74108.0 47 74202.0 48 75330.5 49 75500.0 50 73812.5 51 71667.5 52 69314.5 53 67740.0 54 65613.0 55 60895.5 56 55286.5 57 50420.5 58 46160.0 59 41347.0 60 35207.5 61 28294.5 62 21787.0 63 16572.0 64 12434.0 65 9130.0 66 6662.0 67 5045.5 68 3685.5 69 2666.0 70 1925.0 71 1427.0 72 1041.5 73 778.0 74 616.5 75 446.5 76 280.5 77 145.0 78 91.0 79 53.0 80 37.5 81 31.0 82 16.0 83 11.0 84 9.5 85 5.0 86 4.0 87 5.0 88 4.0 89 2.0 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10568541883742068 2 1.9856349241412997E-4 3 0.0 4 0.18084170071616887 5 0.0 6 1.4892261931059747E-4 7 0.03857095840144475 8 0.0 9 0.0 10-11 5.460496041388575E-4 12-13 4.4676785793179244E-4 14-15 3.226656751729612E-4 16-17 0.004715882944835587 18-19 0.0017374305586236372 20-21 0.0036982450462131706 22-23 0.001514046629657741 24-25 0.005435675604836808 26-27 0.004914446437249717 28-29 0.0037478859193167033 30-31 8.438948427600524E-4 32-33 7.446130965529873E-5 34-35 0.0012162013910365461 36-37 0.0012410218275883123 38-39 5.708700406906237E-4 40-41 0.0017870714317271697 42-43 9.431765889671174E-4 44-45 9.183561524153511E-4 46-47 1.4892261931059747E-4 48-49 2.4820436551766246E-5 50-51 0.0417727947166226 52-53 7.446130965529873E-5 54-55 0.13504799527816017 56-57 7.446130965529873E-5 58-59 0.0 60-61 0.0 62-63 4.96408731035325E-4 64-65 0.061629143958035586 66-67 0.034301843314540954 68-69 0.0019111736144860009 70-71 0.003127375005522547 72-73 0.06778461222287362 74-75 0.004839985127594418 76-77 0.0016629692489683386 78-79 0.0015885079393130397 80-81 1.9856349241412997E-4 82-83 4.964087310353249E-5 84-85 2.4820436551766246E-5 86-87 3.226656751729612E-4 88-89 0.0015636875027612736 90-91 2.7302480206942874E-4 92-93 0.002407582345521326 94-95 0.004219474213800262 96-97 0.0024572232186248584 98-99 0.0 100-101 0.0010672787717259486 102-103 0.001414764883450676 104-105 2.2338392896589622E-4 106-107 4.964087310353249E-5 108-109 0.0 110-111 1.4892261931059747E-4 112-113 4.964087310353249E-5 114-115 1.2410218275883125E-4 116-117 0.0 118-119 9.9281746207065E-4 120-121 0.0016629692489683386 122-123 4.4676785793179244E-4 124-125 0.007669514894495771 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2014469.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.26629583368639 #Duplication Level Percentage of deduplicated Percentage of total 1 82.98329504602634 55.819788738200906 2 10.209284346679368 13.734814822279162 3 2.9912944262230634 6.036398873999333 4 1.3258292801646718 3.5673449833808117 5 0.6689478807160077 2.249882302078026 6 0.3943972177229819 1.5917783954002154 7 0.26974357007427846 1.2701255548695791 8 0.1985979252923279 1.068715743573606 9 0.1375591624010031 0.8327785781429987 >10 0.7606592866881743 9.219694322920809 >50 0.04262284877710643 1.944067770512457 >100 0.017244572005010676 1.9152267089048762 >500 2.2475881268880235E-4 0.0988762508175162 >1k 2.996784169183889E-4 0.6505069549196998 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4665 0.23157467302797907 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4240 0.21047730195897776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2741 0.13606563317678258 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.964087310353249E-5 2 0.0 0.0 0.0 0.0 4.964087310353249E-5 3 0.0 0.0 0.0 0.0 4.964087310353249E-5 4 0.0 0.0 0.0 0.0 4.964087310353249E-5 5 0.0 0.0 0.0 4.964087310353249E-5 4.964087310353249E-5 6 0.0 0.0 0.0 4.964087310353249E-5 4.964087310353249E-5 7 4.964087310353249E-5 0.0 0.0 9.928174620706498E-5 4.964087310353249E-5 8 4.964087310353249E-5 0.0 0.0 9.928174620706498E-5 4.964087310353249E-5 9 4.964087310353249E-5 0.0 0.0 9.928174620706498E-5 4.964087310353249E-5 10-11 4.964087310353249E-5 0.0 0.0 9.928174620706498E-5 4.964087310353249E-5 12-13 4.964087310353249E-5 0.0 0.0 9.928174620706498E-5 4.964087310353249E-5 14-15 4.964087310353249E-5 0.0 0.0 9.928174620706498E-5 4.964087310353249E-5 16-17 4.964087310353249E-5 0.0 0.0 1.9856349241412997E-4 4.964087310353249E-5 18-19 7.446130965529873E-5 0.0 0.0 2.2338392896589624E-4 9.928174620706498E-5 20-21 9.928174620706498E-5 0.0 0.0 3.474861117247275E-4 1.4892261931059747E-4 22-23 9.928174620706498E-5 0.0 0.0 4.4676785793179244E-4 1.4892261931059747E-4 24-25 1.9856349241412997E-4 0.0 0.0 6.453313503459224E-4 1.4892261931059747E-4 26-27 1.9856349241412997E-4 0.0 0.0 7.942539696565199E-4 1.4892261931059747E-4 28-29 3.226656751729612E-4 0.0 0.0 0.0012658422641400786 1.4892261931059747E-4 30-31 3.4748611172472744E-4 0.0 0.0 0.0027302480206942872 1.4892261931059747E-4 32-33 3.4748611172472744E-4 0.0 0.0 0.006006545645527432 1.9856349241412997E-4 34-35 3.9712698482825994E-4 0.0 0.0 0.010176378986224161 1.9856349241412997E-4 36-37 4.96408731035325E-4 0.0 0.0 0.01759768951520227 1.9856349241412997E-4 38-39 6.453313503459224E-4 0.0 0.0 0.03174533834970903 2.2338392896589624E-4 40-41 6.453313503459224E-4 0.0 0.0 0.048126826473874756 2.482043655176625E-4 42-43 7.197926600012211E-4 0.0 0.0 0.06636984733942294 2.482043655176625E-4 44-45 8.935357158635849E-4 0.0 0.0 0.08674742574842303 2.482043655176625E-4 46-47 0.0011169196448294811 0.0 0.0 0.11333011329536469 2.7302480206942874E-4 48-49 0.0011913809544847798 0.0 0.0 0.14095525917748053 2.9784523862119494E-4 50-51 0.0011913809544847798 0.0 0.0 0.17443802808581318 2.9784523862119494E-4 52-53 0.0011913809544847798 0.0 0.0 0.20784633568449057 3.226656751729612E-4 54-55 0.0011913809544847798 0.0 0.0 0.24403453217696575 3.9712698482825994E-4 56-57 0.0011913809544847798 0.0 0.0 0.29034946678256157 4.4676785793179244E-4 58-59 0.0011913809544847798 0.0 0.0 0.3428943309626507 4.4676785793179244E-4 60-61 0.0011913809544847798 0.0 0.0 0.40308388960068386 4.4676785793179244E-4 62-63 0.0012410218275883123 0.0 0.0 0.466028516695963 4.4676785793179244E-4 64-65 0.0012410218275883123 0.0 0.0 0.5287001189891729 4.4676785793179244E-4 66-67 0.0013403035737953773 0.0 0.0 0.6017714841975726 4.4676785793179244E-4 68-69 0.0013899444468989098 0.0 0.0 0.687104145062545 4.4676785793179244E-4 70-71 0.0014395853200024422 0.0 0.0 0.7878254765896124 4.4676785793179244E-4 72-73 0.0014892261931059747 0.0 0.0 0.8926421801477213 4.4676785793179244E-4 74-75 0.0014892261931059747 0.0 0.0 0.9977567289444513 4.4676785793179244E-4 76-77 0.0014892261931059747 0.0 0.0 1.1176394374894825 4.4676785793179244E-4 78-79 0.0014892261931059747 0.0 0.0 1.2585450557938593 4.4676785793179244E-4 80-81 0.0016877896855201047 0.0 0.0 1.416378211826541 4.4676785793179244E-4 82-83 0.0017870714317271697 0.0 0.0 1.5885327597495915 4.4676785793179244E-4 84-85 0.001911173614486001 0.0 0.0 1.7622758156119553 4.4676785793179244E-4 86-87 0.0020352757972448322 0.0 0.0 1.9447556651405407 4.4676785793179244E-4 88-89 0.0020352757972448322 0.0 0.0 2.1515595424898573 4.4676785793179244E-4 90-91 0.00218419841655543 0.0 0.0 2.396686173875101 4.4676785793179244E-4 92-93 0.0022338392896589622 0.0 0.0 2.66114792533417 4.4676785793179244E-4 94-95 0.0022338392896589622 0.0 0.0 2.929059717473935 4.4676785793179244E-4 96-97 0.0022338392896589622 0.0 0.0 3.1950851564357654 4.4676785793179244E-4 98-99 0.0025565049648319234 0.0 0.0 3.4879414873100556 4.4676785793179244E-4 100-101 0.0026806071475907545 0.0 0.0 3.832821453196847 4.4676785793179244E-4 102-103 0.0028543502034531184 0.0 0.0 4.200461759401609 4.4676785793179244E-4 104-105 0.003325938497936677 0.0 0.0 4.571676208469825 4.4676785793179244E-4 106-107 0.0034996815537990406 0.0 0.0 4.9442309611118365 4.4676785793179244E-4 108-109 0.003598963300006106 0.0 0.0 5.333638790172497 4.96408731035325E-4 110-111 0.0036734246096614045 0.0 0.0 5.776360916946352 5.460496041388575E-4 112-113 0.00377270635586847 0.0 0.0 6.259639637045792 5.460496041388575E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3895 0.0 60.34153 1 GTATCAA 6370 0.0 51.69246 1 TATCAAC 8575 0.0 37.58904 2 ATCAACG 8065 0.0 37.528175 3 CAACGCA 8120 0.0 37.289867 5 TCAACGC 8115 0.0 37.07669 4 AACGCAG 8475 0.0 35.65768 6 GTACATG 7125 0.0 29.219187 1 CGCAGAG 10325 0.0 29.203789 8 ACGCAGA 10190 0.0 29.131002 7 TACATGG 7375 0.0 27.79038 2 GCAGAGT 10900 0.0 26.462843 9 ACATGGG 7290 0.0 25.907757 3 GAGTACT 6930 0.0 25.188768 12-13 GTGGTAT 1920 0.0 24.855146 1 AGAGTAC 10325 0.0 24.5099 10-11 TGGTATC 1875 0.0 23.51411 2 AGTACTT 7520 0.0 23.48933 12-13 CAGAGTA 10955 0.0 23.371834 10-11 GTACTTT 7675 0.0 22.976492 14-15 >>END_MODULE