FastQCFastQC Report
Thu 26 May 2016
SRR1364489_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364489_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934182
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42380.21911071450359892No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29030.15008928839168187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20210.10448861585931417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG109500.031.7431451
TACATGG110250.030.2206212
GTATCAA103400.029.9894431
ACATGGG112850.026.7841913
GAGTACT36300.026.0548712-13
CGCATAG2757.4578566E-1125.9898228
TATCAAC122950.025.4053232
ATCAACG118600.024.432093
CAACGCA120400.024.2502125
TCAACGC119500.024.184794
AACGCAG117150.024.0972966
CATGGGG40250.023.835734
GTACTTT42800.022.48376714-15
ACGCATA3455.2750693E-1122.44298
GGTATCA78350.022.3336411
CATGGGA82000.022.309664
ATGGGAG28850.022.0995755
ACGCAGA130200.021.4075577
CGCAGAG127800.021.2048538
AGTACTT42000.020.04036312-13
Produced by FastQC (version 0.11.5)