##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364489_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1934182
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.669713605027862	31.0	26.0	31.0	10.0	34.0
2	26.923687636427182	31.0	26.0	31.0	10.0	34.0
3	26.942357027415206	31.0	26.0	31.0	10.0	34.0
4	29.722441838461943	35.0	28.0	37.0	10.0	37.0
5	30.46184640328573	35.0	30.0	37.0	12.0	37.0
6	30.64750886938251	35.0	30.0	37.0	11.0	37.0
7	30.528239845061115	35.0	30.0	37.0	10.0	37.0
8	30.10923222323442	35.0	30.0	37.0	10.0	37.0
9	30.809489489613696	35.0	29.0	39.0	8.0	39.0
10-11	30.925280299372034	36.0	29.5	39.0	8.0	39.0
12-13	30.769647323778216	35.0	29.5	39.0	8.0	39.0
14-15	31.48510274627724	37.0	29.0	40.0	8.0	41.0
16-17	31.763594377364697	37.0	30.0	40.0	8.0	41.0
18-19	31.70657595820869	37.0	30.0	40.0	7.0	41.0
20-21	31.483963763492785	37.0	29.5	40.0	2.0	41.0
22-23	31.538629767002277	37.0	29.5	40.0	2.0	41.0
24-25	31.553973721190665	37.0	30.0	40.0	2.0	41.0
26-27	31.663549759019574	37.0	30.0	40.0	2.0	41.0
28-29	31.60058231335004	37.0	30.0	40.0	2.0	41.0
30-31	31.24048073035526	37.0	29.0	40.0	2.0	41.0
32-33	31.256056565514516	37.0	30.0	40.0	2.0	41.0
34-35	30.954636895597208	36.0	29.0	40.0	2.0	41.0
36-37	30.714466632405845	36.0	28.0	40.0	2.0	41.0
38-39	30.56239536920517	36.0	28.0	40.0	2.0	41.0
40-41	30.6086684190009	36.0	28.5	40.0	2.0	41.0
42-43	30.458919326102716	36.0	28.0	40.0	2.0	41.0
44-45	30.114057777396336	35.5	27.5	40.0	2.0	41.0
46-47	29.83878171754261	35.0	26.5	40.0	2.0	41.0
48-49	29.570073808979714	35.0	26.0	40.0	2.0	41.0
50-51	29.549688447105805	35.0	26.5	40.0	2.0	41.0
52-53	29.332233988321676	35.0	26.0	40.0	2.0	41.0
54-55	29.20617914963535	35.0	26.0	39.0	2.0	41.0
56-57	28.796402044895466	34.5	25.0	39.0	2.0	41.0
58-59	28.55411745120159	34.0	24.5	39.0	2.0	40.5
60-61	28.124586000696937	34.0	23.0	39.0	2.0	40.0
62-63	28.055595595450686	34.0	23.5	38.0	2.0	40.0
64-65	27.8549903783615	34.0	23.5	38.0	2.0	40.0
66-67	27.61701587544502	33.5	23.0	37.5	2.0	40.0
68-69	27.43413106936162	33.0	23.0	37.0	2.0	40.0
70-71	27.299515764286916	33.0	23.5	37.0	2.0	39.5
72-73	27.249966652569405	33.0	23.5	36.5	2.0	39.0
74-75	26.96295720878387	33.0	23.0	36.0	2.0	39.0
76-77	26.685112362745596	33.0	22.0	36.0	2.0	39.0
78-79	26.367755464583993	33.0	21.5	35.0	2.0	37.5
80-81	26.360666938271578	33.0	23.0	35.0	2.0	37.0
82-83	26.157249938216776	33.0	22.5	35.0	2.0	37.0
84-85	25.96342045371118	33.0	22.0	35.0	2.0	36.0
86-87	25.75424313740899	33.0	20.5	35.0	2.0	36.0
88-89	25.529288867335133	33.0	20.0	35.0	2.0	36.0
90-91	25.363348950615816	32.5	19.5	35.0	2.0	35.0
92-93	25.266794179658376	32.5	19.5	35.0	2.0	35.0
94-95	25.18975773737942	32.5	19.0	35.0	2.0	35.0
96-97	25.05765538093106	32.0	18.0	35.0	2.0	35.0
98-99	24.94806434968374	32.0	17.5	35.0	2.0	35.0
100-101	24.88933952440877	32.0	16.5	35.0	2.0	35.0
102-103	23.726647233817708	30.5	10.0	34.0	2.0	35.0
104-105	24.505246662413363	32.0	10.0	35.0	2.0	35.0
106-107	24.608082900161413	32.0	8.0	35.0	2.0	35.0
108-109	24.533214040871023	32.0	7.0	35.0	2.0	35.0
110-111	24.417574716340035	32.0	4.5	35.0	2.0	35.0
112-113	24.383150603200733	32.0	2.0	35.0	2.0	35.0
114-115	24.316549838639798	32.0	2.0	35.0	2.0	35.0
116-117	24.13233708099858	32.0	2.0	35.0	2.0	35.0
118-119	23.977350114932307	32.0	2.0	35.0	2.0	35.0
120-121	23.82354762892013	32.0	2.0	35.0	2.0	35.0
122-123	23.537350156293463	32.0	2.0	35.0	2.0	35.0
124-125	22.51829481403508	30.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	2.1273113836234216
1101	2	1.689710292609405
1101	3	2.1079978517211373
1101	4	2.5218639622311905
1101	5	1.947941072775805
1101	6	1.9593448008750833
1101	7	1.7791475419664202
1101	8	2.2259026208905297
1101	9	2.8919507350765095
1101	10-11	2.862785834385935
1101	12-13	2.7475109660088
1101	14-15	3.051531102549724
1101	16-17	2.722579107325707
1101	18-19	2.597056866806561
1101	20-21	2.9293254107228144
1101	22-23	2.9024938827547935
1101	24-25	2.8106968784116013
1101	26-27	2.5892355299166994
1101	28-29	2.571631525559642
1101	30-31	2.7225700121896352
1101	32-33	2.996425658564391
1101	34-35	3.1231790303146028
1101	36-37	3.197576315698111
1101	38-39	3.175425030285272
1101	40-41	2.944335421454433
1101	42-43	2.928318689706611
1101	44-45	3.115103759323585
1101	46-47	3.0758425435846313
1101	48-49	3.097007319079715
1101	50-51	3.17084601779613
1101	52-53	3.1038988921674004
1101	54-55	3.1268485127618675
1101	56-57	3.2710585589669208
1101	58-59	3.3389729239236736
1101	60-61	3.58753542537632
1101	62-63	3.211060156677071
1101	64-65	3.174045100052112
1101	66-67	3.1360370423251602
1101	68-69	2.936592956381226
1101	70-71	3.07348158725598
1101	72-73	2.8789533433754038
1101	74-75	2.8665601483778147
1101	76-77	3.0811430706599268
1101	78-79	3.0094650826654252
1101	80-81	2.7958462195407705
1101	82-83	2.7686248889393568
1101	84-85	2.6244149795740874
1101	86-87	2.5517466155405586
1101	88-89	2.7638773484997436
1101	90-91	2.7220131376796353
1101	92-93	2.6783553225006678
1101	94-95	2.545568560344396
1101	96-97	2.596729487650606
1101	98-99	2.6047885540458786
1101	100-101	2.5453848489544733
1101	102-103	2.530080512510086
1101	104-105	2.637678320154638
1101	106-107	2.7184171201217566
1101	108-109	2.755199075411536
1101	110-111	2.819714013316915
1101	112-113	2.7678455491101808
1101	114-115	2.7127908018393896
1101	116-117	2.828956643963835
1101	118-119	2.766026009404701
1101	120-121	2.7616714283274035
1101	122-123	2.7485140223837163
1101	124-125	2.768671289859121
1102	1	1.3011606599591978
1102	2	1.0154734593790131
1102	3	1.2277521232861979
1102	4	1.7177060003609235
1102	5	1.6903630062419879
1102	6	1.6192391709950975
1102	7	1.50832793921251
1102	8	1.615373519180217
1102	9	2.151858672653699
1102	10-11	1.6530168230404918
1102	12-13	1.8869574633669792
1102	14-15	2.1874497374952924
1102	16-17	1.7479104557982836
1102	18-19	1.924395875392129
1102	20-21	2.0021490606340997
1102	22-23	1.9967046121113121
1102	24-25	1.7263745389246985
1102	26-27	1.7499778661098802
1102	28-29	1.512979720974588
1102	30-31	1.4626888751935851
1102	32-33	1.2670191010823189
1102	34-35	1.8839510574534017
1102	36-37	1.985360524536862
1102	38-39	2.0127274919375218
1102	40-41	1.6257853318153863
1102	42-43	1.6925856312598846
1102	44-45	1.982556281581818
1102	46-47	1.8540524739926347
1102	48-49	2.0416691879570337
1102	50-51	1.8925398857045295
1102	52-53	2.0989660753465174
1102	54-55	1.7818375676633735
1102	56-57	1.9316516854860453
1102	58-59	2.05427595868775
1102	60-61	2.348931415340715
1102	62-63	2.1251349051695243
1102	64-65	2.140537147721126
1102	66-67	2.2339110929671513
1102	68-69	1.9973589331674972
1102	70-71	2.235325497435962
1102	72-73	2.0621353693634497
1102	74-75	2.130298519918423
1102	76-77	2.0638038496109985
1102	78-79	2.056139939417129
1102	80-81	1.6023661915818899
1102	82-83	1.6771707368606705
1102	84-85	1.7216007891355218
1102	86-87	1.8529752253604421
1102	88-89	1.5630238713211675
1102	90-91	1.489642932031277
1102	92-93	1.6084087715952542
1102	94-95	1.7606082268222174
1102	96-97	1.7755624857340102
1102	98-99	1.839197599074847
1102	100-101	1.7275453252261421
1102	102-103	1.5033327236658778
1102	104-105	1.5112178637380502
1102	106-107	1.9052279555805356
1102	108-109	1.9562374064978236
1102	110-111	1.667014116302326
1102	112-113	1.734468903449347
1102	114-115	1.718808853459791
1102	116-117	1.9804923604026854
1102	118-119	2.0784390355871984
1102	120-121	2.0926656694918684
1102	122-123	2.0716536980610947
1102	124-125	1.9703919303078123
1103	1	1.7018724909525034
1103	2	1.3206482537658495
1103	3	1.4066657059242687
1103	4	2.1741571993773157
1103	5	1.5188683915289651
1103	6	1.6943880974892842
1103	7	1.5641426050512557
1103	8	1.8317421124399758
1103	9	2.4808255503604464
1103	10-11	2.0758885025508143
1103	12-13	2.0705565752367043
1103	14-15	2.39617224286053
1103	16-17	2.2915375409131897
1103	18-19	1.9086918546807468
1103	20-21	2.44103352456165
1103	22-23	2.222387683818514
1103	24-25	2.2235051162157724
1103	26-27	2.136500484997768
1103	28-29	1.9023527248092371
1103	30-31	2.1104224429384715
1103	32-33	1.961349378206009
1103	34-35	1.9326073114062652
1103	36-37	2.2692247039154587
1103	38-39	2.3077188433541345
1103	40-41	2.346933096632796
1103	42-43	2.3768398661220935
1103	44-45	2.350777478407533
1103	46-47	2.541405811232355
1103	48-49	2.561455395487627
1103	50-51	2.4132600101229755
1103	52-53	2.4048262291716362
1103	54-55	2.391770032135284
1103	56-57	2.57815014508045
1103	58-59	2.615368030851638
1103	60-61	2.6197922698834653
1103	62-63	2.4685240342553634
1103	64-65	2.306384692895012
1103	66-67	2.3556317184466913
1103	68-69	2.1949617322838044
1103	70-71	2.3642212261727025
1103	72-73	2.3479593717047216
1103	74-75	2.396531705094951
1103	76-77	2.4183516137031553
1103	78-79	2.556605271385571
1103	80-81	2.0656258702841903
1103	82-83	2.1261100188778705
1103	84-85	2.057215898698111
1103	86-87	2.1789165920392612
1103	88-89	2.185543389560195
1103	90-91	2.2122189023012275
1103	92-93	2.1218733222155457
1103	94-95	1.9632485671256816
1103	96-97	2.1405320114494977
1103	98-99	2.0900884810213114
1103	100-101	1.9105012593659403
1103	102-103	1.9387478354042074
1103	104-105	2.095049289633373
1103	106-107	1.8577860122228351
1103	108-109	2.1759427275363556
1103	110-111	2.3484840917782748
1103	112-113	2.175266475830707
1103	114-115	2.0823308159981693
1103	116-117	2.2314978422116525
1103	118-119	2.3016387192083876
1103	120-121	2.3246700248199126
1103	122-123	2.2866061911120426
1103	124-125	2.223518389469625
1104	1	1.4077248872807928
1104	2	1.2613947763713682
1104	3	1.3324646828627529
1104	4	1.6127111290421894
1104	5	1.1905099785320274
1104	6	1.1018553488474012
1104	7	1.0851465153035988
1104	8	1.3777319822798333
1104	9	1.905168942090686
1104	10-11	1.7606012933311455
1104	12-13	1.3289236585554427
1104	14-15	1.6199627977793511
1104	16-17	1.304511428885185
1104	18-19	0.8128782672070045
1104	20-21	-3.660250203567358
1104	22-23	-0.4765421764983735
1104	24-25	0.8404843850125623
1104	26-27	0.966133066693935
1104	28-29	0.7732366257370877
1104	30-31	1.5182879701157077
1104	32-33	1.2642717586741554
1104	34-35	1.4516837408701235
1104	36-37	1.72569603281595
1104	38-39	1.716396618267236
1104	40-41	1.6926712415727891
1104	42-43	1.6635667092641988
1104	44-45	1.8473668176585356
1104	46-47	1.7969581285535732
1104	48-49	1.9433128433264528
1104	50-51	1.711242322342457
1104	52-53	1.773512068840958
1104	54-55	1.6208728047142245
1104	56-57	1.91300365390763
1104	58-59	1.9949272662025699
1104	60-61	2.1519464853590904
1104	62-63	1.8220591397422545
1104	64-65	1.9133910156881981
1104	66-67	1.7977385357580893
1104	68-69	1.7649196444638378
1104	70-71	1.9539797655080342
1104	72-73	1.8661979333507865
1104	74-75	1.8541895577108214
1104	76-77	1.7996420273570983
1104	78-79	1.8300094187603833
1104	80-81	1.5555506971157271
1104	82-83	1.5078341005194495
1104	84-85	1.491637248388333
1104	86-87	1.4423529579329148
1104	88-89	1.6155241613342781
1104	90-91	1.6308374715340825
1104	92-93	1.3398430046460241
1104	94-95	1.4405992501489635
1104	96-97	1.6074866120567215
1104	98-99	1.4258841607666426
1104	100-101	0.9283639754227231
1104	102-103	1.3205577898895768
1104	104-105	1.507105978672211
1104	106-107	1.5687849564324239
1104	108-109	1.5344519769935232
1104	110-111	1.6089407813841952
1104	112-113	1.5862752731389982
1104	114-115	1.4817937664230065
1104	116-117	1.5598433945350507
1104	118-119	1.6054280812237884
1104	120-121	1.5264601191848506
1104	122-123	1.4429877788401662
1104	124-125	1.6128133572197143
1105	1	1.8201432939553293
1105	2	1.5312024661273114
1105	3	1.4348948496531442
1105	4	2.1407842526258563
1105	5	1.661419558125118
1105	6	1.5589888368084779
1105	7	1.491915067315805
1105	8	1.809789257839622
1105	9	2.2995844865507244
1105	10-11	1.9404985103505972
1105	12-13	1.9655002829670885
1105	14-15	2.118449504466547
1105	16-17	2.0920843201569284
1105	18-19	1.8133459254483455
1105	20-21	2.0596278586478043
1105	22-23	1.9514196307433203
1105	24-25	1.841974020259709
1105	26-27	1.8805815925547122
1105	28-29	1.3464893515834113
1105	30-31	1.7715455057496428
1105	32-33	1.818010849091472
1105	34-35	1.5382418142724674
1105	36-37	1.8825212356092855
1105	38-39	1.6762569554481566
1105	40-41	1.8859046130641772
1105	42-43	2.0154296739304662
1105	44-45	2.090014328012284
1105	46-47	2.2633628010009694
1105	48-49	2.03351719038287
1105	50-51	2.182065910009939
1105	52-53	2.1111901716451307
1105	54-55	2.1325259449675684
1105	56-57	2.38925795054665
1105	58-59	2.1923526926366357
1105	60-61	2.468864841271646
1105	62-63	2.2804302531206524
1105	64-65	1.871127321448327
1105	66-67	2.2227234383120624
1105	68-69	1.7614976081277192
1105	70-71	1.8401710977482288
1105	72-73	1.7357727660239775
1105	74-75	2.0750323032684292
1105	76-77	1.9465783129975875
1105	78-79	1.8846830027711547
1105	80-81	1.8778386110431597
1105	82-83	1.9319329570839905
1105	84-85	1.8899942265868042
1105	86-87	1.967880062207282
1105	88-89	2.024942468130636
1105	90-91	1.9691575822373721
1105	92-93	1.9365888582871484
1105	94-95	1.789887188660213
1105	96-97	1.9741076323714921
1105	98-99	1.768593356955062
1105	100-101	1.913343983842939
1105	102-103	1.900254664237373
1105	104-105	1.982338658319474
1105	106-107	2.0116462019802057
1105	108-109	2.115513201701635
1105	110-111	1.9593272770311678
1105	112-113	1.8784746534416819
1105	114-115	1.8349834856974283
1105	116-117	1.9670428319256743
1105	118-119	1.9990314854342905
1105	120-121	1.9787895283308323
1105	122-123	2.1039601376820016
1105	124-125	1.905865610789327
1106	1	-0.4464357423350265
1106	2	-0.22052608210329794
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2114	102-103	-1.3882465298035598
2114	104-105	-1.2913600158277063
2114	106-107	-6.998035913872489
2114	108-109	-4.705151558256848
2114	110-111	-6.467167421754834
2114	112-113	-2.4045034883533027
2114	114-115	-1.321717860315733
2114	116-117	-1.163602824122755
2114	118-119	-1.2143935393844068
2114	120-121	-1.397044677189701
2114	122-123	-4.210916717264958
2114	124-125	-4.974319268482624
2115	1	-0.9025742768651277
2115	2	-1.2138996386942615
2115	3	-0.6613162549430349
2115	4	-1.736418246112823
2115	5	-1.7854128622713041
2115	6	-1.0039819526861002
2115	7	-1.2711176452097313
2115	8	-1.159424287582766
2115	9	-2.0028954827123506
2115	10-11	-0.863495344670909
2115	12-13	-1.7571191294218487
2115	14-15	-1.5271158399722893
2115	16-17	-1.863050458688818
2115	18-19	-1.3398002778757672
2115	20-21	-0.7033312601200912
2115	22-23	-0.8297683810541407
2115	24-25	-1.1469971430189752
2115	26-27	-0.9198058675502452
2115	28-29	-1.2977085927644687
2115	30-31	-1.1105148828414393
2115	32-33	-1.0201224883593696
2115	34-35	-0.6786126200823013
2115	36-37	-0.9495106379073057
2115	38-39	-1.4440385585811804
2115	40-41	-5.600870368106243
2115	42-43	-2.6066916470834975
2115	44-45	-5.434509738951224
2115	46-47	-2.23007883213074
2115	48-49	-1.7384994750939953
2115	50-51	-1.43762220485889
2115	52-53	-1.2174590961471132
2115	54-55	-1.8367435477528673
2115	56-57	-2.0317549923293505
2115	58-59	-1.63524119655483
2115	60-61	-1.7112381338412312
2115	62-63	-2.1336269767541616
2115	64-65	-2.023395077122629
2115	66-67	-4.656750641044066
2115	68-69	-3.7054276125933967
2115	70-71	-6.043139883402947
2115	72-73	-3.358486198979083
2115	74-75	-2.208762571878861
2115	76-77	-6.063968354267985
2115	78-79	-6.827070000535954
2115	80-81	-3.6237569898548863
2115	82-83	-2.465546526901445
2115	84-85	-2.6421574222743125
2115	86-87	-2.8136903563908042
2115	88-89	-5.194082079698891
2115	90-91	-6.429252150908056
2115	92-93	-7.095793798772359
2115	94-95	-5.672543799645801
2115	96-97	-8.310908457991214
2115	98-99	-5.309518896987118
2115	100-101	-6.922590765164248
2115	102-103	-8.27303607562253
2115	104-105	-7.171351878124877
2115	106-107	-9.150853527307062
2115	108-109	-6.021451097906372
2115	110-111	-3.5828710226522418
2115	112-113	-3.491425920790153
2115	114-115	-3.5181441286857194
2115	116-117	-7.2333061468656865
2115	118-119	-5.404534021833246
2115	120-121	-5.706415379149579
2115	122-123	-5.025242827885741
2115	124-125	-3.924265057233441
2116	1	-5.112030446833465
2116	2	-9.605565312991665
2116	3	-9.695955779726582
2116	4	-6.297899900123863
2116	5	-4.990530335442308
2116	6	-4.571587692549926
2116	7	-4.737896624727231
2116	8	-11.249331191670862
2116	9	-6.427805819230603
2116	10-11	-8.582303573396846
2116	12-13	-6.324057646892218
2116	14-15	-4.539069803378382
2116	16-17	-5.61007991804604
2116	18-19	-4.674907471268689
2116	20-21	-4.7860178021918784
2116	22-23	-7.4881238091972655
2116	24-25	-8.951901577858841
2116	26-27	-5.886634757404263
2116	28-29	-5.364487309595983
2116	30-31	-4.866243626291801
2116	32-33	-5.114534457033187
2116	34-35	-4.767701695147451
2116	36-37	-5.358282861141358
2116	38-39	-5.5503029187481765
2116	40-41	-5.898772032484622
2116	42-43	-7.85123303238429
2116	44-45	-6.985652072723532
2116	46-47	-5.797561547393148
2116	48-49	-5.549260056085338
2116	50-51	-5.783226330079543
2116	52-53	-5.761323276987149
2116	54-55	-6.392624736634595
2116	56-57	-9.400828358772792
2116	58-59	-7.1822733703338635
2116	60-61	-6.41586000612681
2116	62-63	-6.039361236610137
2116	64-65	-6.173746025594145
2116	66-67	-8.308981983334409
2116	68-69	-7.191069196835038
2116	70-71	-5.947488912515556
2116	72-73	-6.286759791682716
2116	74-75	-6.390525510039076
2116	76-77	-6.542339666156806
2116	78-79	-12.118307759369207
2116	80-81	-9.899455121632183
2116	82-83	-9.610894183041992
2116	84-85	-8.472855869274792
2116	86-87	-7.37264409563819
2116	88-89	-7.287244976529848
2116	90-91	-7.5557928041905775
2116	92-93	-7.99192163227848
2116	94-95	-8.154639123134828
2116	96-97	-8.887146934832817
2116	98-99	-13.235638026831047
2116	100-101	-10.820338933367337
2116	102-103	-11.733935705060173
2116	104-105	-12.74097154384821
2116	106-107	-11.518991731655738
2116	108-109	-10.232208876913909
2116	110-111	-13.437863982667931
2116	112-113	-12.934549152037878
2116	114-115	-12.474127589976312
2116	116-117	-14.260473568900487
2116	118-119	-14.043518110067716
2116	120-121	-11.814032134312237
2116	122-123	-10.617694283516329
2116	124-125	-9.96336586560577
2201	1	2.265916780463961
2201	2	1.9599464180237298
2201	3	2.1888054790213047
2201	4	2.5340903655129345
2201	5	1.9406751957401127
2201	6	1.566576194302808
2201	7	1.665867019062727
2201	8	2.1340203303433682
2201	9	2.7147355452030943
2201	10-11	2.382365882656746
2201	12-13	2.362631498066559
2201	14-15	2.6754025516500874
2201	16-17	2.4953396136797608
2201	18-19	2.2394872627662963
2201	20-21	2.4680566446716448
2201	22-23	2.241452159771658
2201	24-25	2.250398891400007
2201	26-27	2.077026742582966
2201	28-29	2.1166165769467575
2201	30-31	2.399880764683701
2201	32-33	2.3139930302119396
2201	34-35	2.5304463415690748
2201	36-37	2.4632307356953653
2201	38-39	2.532373867024713
2201	40-41	2.5162328087131485
2201	42-43	2.484301860708211
2201	44-45	2.7807937541822234
2201	46-47	2.8366349619300344
2201	48-49	2.836646257620636
2201	50-51	2.7168206801389374
2201	52-53	2.6308400683078617
2201	54-55	2.625210541383268
2201	56-57	2.700933834937114
2201	58-59	2.7943358264831595
2201	60-61	2.9941110378578877
2201	62-63	2.5976277936550964
2201	64-65	2.3125345122437793
2201	66-67	2.294434970066103
2201	68-69	2.1132841887212344
2201	70-71	2.283450124861041
2201	72-73	2.404449855885378
2201	74-75	2.505450050573451
2201	76-77	2.643919605967742
2201	78-79	2.4126320871529714
2201	80-81	2.173886802319984
2201	82-83	2.1791001734525075
2201	84-85	2.045563190183504
2201	86-87	2.102956176417063
2201	88-89	1.8949887963404812
2201	90-91	2.154378030373568
2201	92-93	2.243932793542701
2201	94-95	2.303848289750963
2201	96-97	2.1664490448760922
2201	98-99	2.186517666909854
2201	100-101	2.224532804522376
2201	102-103	2.273220503798761
2201	104-105	2.176349846952238
2201	106-107	2.097984678217749
2201	108-109	2.130450570350771
2201	110-111	2.244956762639479
2201	112-113	2.048870429376297
2201	114-115	2.11093788471635
2201	116-117	2.0545099951874874
2201	118-119	2.1010607627081583
2201	120-121	1.9918111617684886
2201	122-123	2.0205834535382508
2201	124-125	1.9968801283258664
2202	1	1.781257289875338
2202	2	1.4666779811291093
2202	3	1.550640967580243
2202	4	2.3234041057466293
2202	5	1.6667542866020355
2202	6	1.5835929171686622
2202	7	1.0656597506045529
2202	8	1.422730287116007
2202	9	1.945459803455467
2202	10-11	-0.7099504092736808
2202	12-13	1.7445785316299798
2202	14-15	2.080959169361428
2202	16-17	1.7763820305510833
2202	18-19	1.9550108103212764
2202	20-21	1.6715426399556783
2202	22-23	1.8102586846037845
2202	24-25	1.7780957095066903
2202	26-27	1.8178930163653462
2202	28-29	1.806783577452098
2202	30-31	2.0368274532750092
2202	32-33	1.88498768736725
2202	34-35	1.6574462063224473
2202	36-37	1.9403459768922922
2202	38-39	2.103831795948178
2202	40-41	1.9685359993815936
2202	42-43	1.9259723418121233
2202	44-45	2.12735454676908
2202	46-47	2.151014628034325
2202	48-49	2.1198892565240683
2202	50-51	2.2750741296680914
2202	52-53	1.9154580196473816
2202	54-55	1.871737867836245
2202	56-57	2.169384078715577
2202	58-59	2.2399738159308527
2202	60-61	2.403030941695679
2202	62-63	2.1251032043830627
2202	64-65	2.1612631446767097
2202	66-67	1.8466611151349355
2202	68-69	1.866542365300667
2202	70-71	2.1932754570900244
2202	72-73	1.8689272714003913
2202	74-75	2.030634605168384
2202	76-77	1.8043280178466716
2202	78-79	2.074342508239422
2202	80-81	1.883736737806359
2202	82-83	1.7000897794263459
2202	84-85	1.7432167984766131
2202	86-87	1.7381381873225727
2202	88-89	1.705552599234057
2202	90-91	1.7375111537400905
2202	92-93	1.708990244836091
2202	94-95	1.600418290734872
2202	96-97	1.679649849686701
2202	98-99	1.6293899562599599
2202	100-101	1.8670797231974738
2202	102-103	2.000097308389556
2202	104-105	2.073057902593991
2202	106-107	1.9214893776812296
2202	108-109	1.714078172024582
2202	110-111	1.4616889191627074
2202	112-113	1.47976862157984
2202	114-115	1.682353802727718
2202	116-117	1.673905062185021
2202	118-119	1.6807872727673612
2202	120-121	1.8922007663602507
2202	122-123	2.075010509706402
2202	124-125	2.0752201137525788
2203	1	1.4715665707564156
2203	2	1.4724082621114398
2203	3	1.559651267884135
2203	4	2.028017939687267
2203	5	1.4195683617115407
2203	6	1.3053573773286793
2203	7	1.1614843776190575
2203	8	1.4827082216111762
2203	9	2.0077094899403285
2203	10-11	1.8598846780349696
2203	12-13	1.8267082670674704
2203	14-15	1.9974251907194613
2203	16-17	1.795545027304371
2203	18-19	1.7449113380261139
2203	20-21	1.8679209756976256
2203	22-23	1.7030843929770576
2203	24-25	1.9062565580073176
2203	26-27	1.4975295228971106
2203	28-29	1.2848816242998744
2203	30-31	1.6805635171378555
2203	32-33	1.6707823830548278
2203	34-35	1.8625082321321713
2203	36-37	2.078673603176103
2203	38-39	1.7967278781156644
2203	40-41	1.432354368366454
2203	42-43	1.6753840488905745
2203	44-45	1.6818857994401526
2203	46-47	1.968619056739822
2203	48-49	2.137985140215978
2203	50-51	1.9268892629049077
2203	52-53	1.910413505099445
2203	54-55	1.7758640383402664
2203	56-57	1.9096261527327663
2203	58-59	2.2694122961724936
2203	60-61	2.4373147705385456
2203	62-63	2.1101636763148512
2203	64-65	2.004018150508685
2203	66-67	1.9125867968139474
2203	68-69	1.8577647631468892
2203	70-71	1.9830232269793342
2203	72-73	2.0902909340637983
2203	74-75	1.9296381836900665
2203	76-77	1.9212368396214785
2203	78-79	2.006964805445204
2203	80-81	1.7718319398959643
2203	82-83	1.713195172675814
2203	84-85	1.6185494357427679
2203	86-87	1.532444145228041
2203	88-89	1.6963608305352516
2203	90-91	1.7782830804040692
2203	92-93	1.7109098538988157
2203	94-95	1.5769257495680655
2203	96-97	1.5939843844810717
2203	98-99	1.7038018057954822
2203	100-101	1.7201211159630212
2203	102-103	1.5604327005563476
2203	104-105	1.6836971885728396
2203	106-107	1.8793356721974206
2203	108-109	1.7746762879092444
2203	110-111	1.9297114155453912
2203	112-113	1.8711642801019082
2203	114-115	1.709327885183626
2203	116-117	1.8253224114402329
2203	118-119	1.894179311564777
2203	120-121	1.9489464594445955
2203	122-123	1.9372576977133562
2203	124-125	1.6851630585988957
2204	1	1.606208221523687
2204	2	1.4237410287168046
2204	3	1.3928369715550808
2204	4	1.9601593812240097
2204	5	1.3311782345036285
2204	6	1.2677190355900159
2204	7	1.188571446832519
2204	8	1.4347194474131868
2204	9	2.112935558747221
2204	10-11	1.826114870701236
2204	12-13	1.7631165775766213
2204	14-15	2.142833610835673
2204	16-17	1.7246945784889576
2204	18-19	1.8624395777749854
2204	20-21	1.9216449498626496
2204	22-23	1.8633686788509642
2204	24-25	2.025470271804597
2204	26-27	1.6704587672923878
2204	28-29	1.7176075837121267
2204	30-31	2.0441632147413316
2204	32-33	1.6616866434132334
2204	34-35	1.8394092879710158
2204	36-37	2.109619807351965
2204	38-39	2.052081524440876
2204	40-41	2.065263388560062
2204	42-43	1.8197829051536623
2204	44-45	1.9870280273438148
2204	46-47	2.1079429043894073
2204	48-49	2.1794003585188264
2204	50-51	2.0103197238110866
2204	52-53	2.007548724948691
2204	54-55	2.069775366229784
2204	56-57	2.320816024445776
2204	58-59	2.316630475395332
2204	60-61	2.635608065432937
2204	62-63	2.2655502153785143
2204	64-65	1.9936137641871348
2204	66-67	2.055964990447137
2204	68-69	2.088948937841849
2204	70-71	2.1867106866998007
2204	72-73	2.107337858629922
2204	74-75	2.113595735595169
2204	76-77	2.218120090685794
2204	78-79	2.1276316624373024
2204	80-81	1.899702009224555
2204	82-83	1.8788849345693457
2204	84-85	1.8576050859654032
2204	86-87	1.8576078760823869
2204	88-89	1.7741155681729133
2204	90-91	1.932252374708792
2204	92-93	1.9326102565985508
2204	94-95	1.3758774621358754
2204	96-97	1.8392264610165405
2204	98-99	2.07591389345734
2204	100-101	1.919090958303041
2204	102-103	1.9196505055008153
2204	104-105	2.0958325804796765
2204	106-107	2.094357532008292
2204	108-109	2.1355079824471197
2204	110-111	2.2814854461495493
2204	112-113	2.085804745583541
2204	114-115	2.0165855186992587
2204	116-117	2.1534079351092004
2204	118-119	2.216425657252696
2204	120-121	2.132978622743817
2204	122-123	2.0165814518995617
2204	124-125	1.5699112894857414
2205	1	1.4757567345724816
2205	2	1.3929885417823122
2205	3	1.459363242469827
2205	4	1.7634722694802356
2205	5	1.2769783763319893
2205	6	1.229457394605049
2205	7	0.9169728475775187
2205	8	1.399619207060347
2205	9	1.8601415899472862
2205	10-11	1.3538611563892324
2205	12-13	1.4524367109308791
2205	14-15	1.603840524914041
2205	16-17	1.2907327853217723
2205	18-19	-1.0308191686871737
2205	20-21	0.21911246489473868
2205	22-23	0.7134037994951434
2205	24-25	1.30524782441665
2205	26-27	1.0217112805999236
2205	28-29	0.8525285959073265
2205	30-31	-4.59154991124128
2205	32-33	-3.581999563398739
2205	34-35	-3.8687108090036375
2205	36-37	0.17196437733787917
2205	38-39	1.213081894097595
2205	40-41	1.3430397373458298
2205	42-43	0.8488655335236324
2205	44-45	1.418359950641893
2205	46-47	1.4614467855678903
2205	48-49	1.776867797579193
2205	50-51	1.308476845064348
2205	52-53	1.3724590012535174
2205	54-55	1.3987615430654827
2205	56-57	1.5055232087881336
2205	58-59	1.577684904362524
2205	60-61	1.8264017789678668
2205	62-63	1.391075804067146
2205	64-65	1.4164952979998695
2205	66-67	1.045268334049677
2205	68-69	0.9345101920004879
2205	70-71	1.5083830653550443
2205	72-73	1.3090848502037922
2205	74-75	1.0801285617846617
2205	76-77	1.3401842446852612
2205	78-79	1.519038923641343
2205	80-81	1.2620846518989133
2205	82-83	1.1535440005902977
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2212	58-59	-3.6271405358455837
2212	60-61	-2.0874048948327975
2212	62-63	-0.8181336221746172
2212	64-65	-1.022468477663132
2212	66-67	-0.7238498974811272
2212	68-69	-0.635352840400266
2212	70-71	-0.15601131828859138
2212	72-73	-0.4097074754151997
2212	74-75	-0.4092014273236373
2212	76-77	-0.2776703240002938
2212	78-79	-0.16832587817344802
2212	80-81	-0.36093383192238093
2212	82-83	-0.33528030233858885
2212	84-85	-0.6574082911779229
2212	86-87	-0.7675954481414813
2212	88-89	-0.4342483008366962
2212	90-91	-0.38500860672730397
2212	92-93	-0.3800716587333106
2212	94-95	-0.36973252338908935
2212	96-97	-0.38613834436096894
2212	98-99	-0.3927823700898436
2212	100-101	-0.3993030475713759
2212	102-103	-0.3703412512054811
2212	104-105	-0.682834983637747
2212	106-107	-0.6379853136032558
2212	108-109	-0.56082026002397
2212	110-111	-0.4583915085532517
2212	112-113	-0.6600839438163071
2212	114-115	-0.598458181682723
2212	116-117	-0.6314248543481256
2212	118-119	-0.5882225278409514
2212	120-121	-0.42534830354232867
2212	122-123	-0.44186885601359194
2212	124-125	-0.3441145097956273
2213	1	-2.1957007416660623
2213	2	-0.8088365125375283
2213	3	-0.6401123356945959
2213	4	-0.8287308207105397
2213	5	-1.0551123860307996
2213	6	-1.860148670828977
2213	7	-1.3515779215066352
2213	8	-1.6152286711439778
2213	9	-0.4335692733703276
2213	10-11	-0.9448682704495859
2213	12-13	-1.331152745887497
2213	14-15	-0.9488235875498106
2213	16-17	-1.0850800181446267
2213	18-19	-0.9416112003636528
2213	20-21	-0.40172464946902053
2213	22-23	-0.36353048750799744
2213	24-25	-0.39694105896556664
2213	26-27	-0.4970812015465853
2213	28-29	-0.5845095070135322
2213	30-31	-0.4308080484070942
2213	32-33	-0.45258751284282184
2213	34-35	-0.2411729005657648
2213	36-37	-0.33705845746200325
2213	38-39	-0.6197905785945004
2213	40-41	-0.35329146139772405
2213	42-43	-0.4187593160485541
2213	44-45	-0.23033939588269803
2213	46-47	-0.3338369969025301
2213	48-49	-0.17607545516283096
2213	50-51	-0.36060359364223515
2213	52-53	-0.40806391131856046
2213	54-55	-2.4198988207992684
2213	56-57	-1.5577885582403574
2213	58-59	-0.4989464803835837
2213	60-61	-0.01144659273833426
2213	62-63	-0.8168785603387541
2213	64-65	-0.6946768623649398
2213	66-67	-0.4134736182067975
2213	68-69	-0.7006388440965168
2213	70-71	-0.5825363636941425
2213	72-73	-0.5218329793199636
2213	74-75	-0.47650418948514783
2213	76-77	-0.5376111201755194
2213	78-79	-0.732486712951939
2213	80-81	-0.9276047903725981
2213	82-83	-0.8483180191401232
2213	84-85	-0.6162140055547916
2213	86-87	-0.5083194986128525
2213	88-89	-0.49622830188495115
2213	90-91	-0.46816855960124215
2213	92-93	-0.44232511855652135
2213	94-95	-0.5319676386861474
2213	96-97	-0.7472936393204392
2213	98-99	-0.6785308381509978
2213	100-101	-0.4986779663883034
2213	102-103	-0.41536111114901075
2213	104-105	-0.37263132521509945
2213	106-107	-0.3612940280507644
2213	108-109	-0.316418758735292
2213	110-111	-0.3904529547399811
2213	112-113	-0.3988624490450654
2213	114-115	-0.6055607036562733
2213	116-117	-0.4356602750074785
2213	118-119	-0.3663346596116206
2213	120-121	-0.29852780871867424
2213	122-123	-0.24435414051326632
2213	124-125	-0.43765621111939623
2214	1	-0.8806016846605687
2214	2	-0.7358661207250563
2214	3	-0.9434789876082696
2214	4	-5.9161386201206305
2214	5	-2.2454437716036573
2214	6	-1.7274479120933641
2214	7	-1.2199740650878361
2214	8	-0.908021391686276
2214	9	-0.5649805509955783
2214	10-11	-0.7502551887718631
2214	12-13	-0.9825449270642999
2214	14-15	-0.9910306812584864
2214	16-17	-1.2005299183213438
2214	18-19	-0.6600615733466597
2214	20-21	-0.3560892703542251
2214	22-23	-0.32649689218402855
2214	24-25	-0.48822692547235036
2214	26-27	-0.8524202235257121
2214	28-29	-0.7060544587431004
2214	30-31	-0.650104226209578
2214	32-33	-0.6720972761530319
2214	34-35	-0.32843173857160934
2214	36-37	-0.384968770589321
2214	38-39	-0.4770844150279423
2214	40-41	-0.7131995989331301
2214	42-43	-0.6746276140918148
2214	44-45	-0.526987728068594
2214	46-47	-0.4490319190312597
2214	48-49	-0.4615223450670527
2214	50-51	-0.5586372513654574
2214	52-53	-0.5853844314530825
2214	54-55	-0.650059816049783
2214	56-57	-0.5549348530336857
2214	58-59	-0.7696102314976905
2214	60-61	-0.4802226474021474
2214	62-63	-0.8341715358073607
2214	64-65	-0.9510733292218809
2214	66-67	-0.8514437921910272
2214	68-69	-0.8143061227863839
2214	70-71	-0.9959976524074214
2214	72-73	-0.8739099069421066
2214	74-75	-0.8309785730389763
2214	76-77	-0.8678333039486681
2214	78-79	-3.9212938699705013
2214	80-81	-1.9789778481958038
2214	82-83	-1.3355442294761097
2214	84-85	-3.3791663781596775
2214	86-87	-1.7048210114584599
2214	88-89	-1.2969013342414009
2214	90-91	-3.0835576644844522
2214	92-93	-2.500120901629394
2214	94-95	-1.4309244121459095
2214	96-97	-1.1980701478534606
2214	98-99	-1.190873783211611
2214	100-101	-1.295165229851925
2214	102-103	-1.022352652686891
2214	104-105	-1.146494978394454
2214	106-107	-1.0614108688437796
2214	108-109	-0.8712201537052913
2214	110-111	-0.8421244734747084
2214	112-113	-0.8418607818570258
2214	114-115	-0.9359090311958624
2214	116-117	-1.0656805563173926
2214	118-119	-0.8567473711779492
2214	120-121	-0.9148756683155916
2214	122-123	-0.7398398700728279
2214	124-125	-0.8564674856609038
2215	1	-1.7931021658967552
2215	2	-1.512442842973126
2215	3	-1.509669697934882
2215	4	-6.656511531418079
2215	5	-3.7457023741817643
2215	6	-2.6976807864098724
2215	7	-2.0549766005150722
2215	8	-1.857925089450152
2215	9	-1.7363320932063182
2215	10-11	-1.6382451284605928
2215	12-13	-1.718806871346498
2215	14-15	-5.020846370548206
2215	16-17	-4.886087772810917
2215	18-19	-3.114024649606371
2215	20-21	-1.7404346296424116
2215	22-23	-1.7319252363905484
2215	24-25	-1.7467750551410255
2215	26-27	-1.9685056668734902
2215	28-29	-2.2678575049956713
2215	30-31	-2.0279466821310947
2215	32-33	-2.2414007321438447
2215	34-35	-1.7638022134389786
2215	36-37	-1.8234543560530199
2215	38-39	-1.9709619907415217
2215	40-41	-2.377133834020583
2215	42-43	-2.3125016171079302
2215	44-45	-2.116449040528927
2215	46-47	-2.272604462499263
2215	48-49	-2.1794066767867335
2215	50-51	-2.913821017591367
2215	52-53	-2.754198896477938
2215	54-55	-2.7016477854750605
2215	56-57	-2.33036683813571
2215	58-59	-2.1061779120052613
2215	60-61	-2.1114753354500024
2215	62-63	-2.12912119575212
2215	64-65	-1.9079419170934102
2215	66-67	-2.1133322509922614
2215	68-69	-2.142503483522134
2215	70-71	-1.9779598200932078
2215	72-73	-2.0217971975768627
2215	74-75	-2.0899235163191037
2215	76-77	-2.021607295635107
2215	78-79	-4.189932835032202
2215	80-81	-4.136323217383705
2215	82-83	-2.940264194013128
2215	84-85	-5.791411391727788
2215	86-87	-3.599573502061979
2215	88-89	-4.878398435274601
2215	90-91	-6.601365218407654
2215	92-93	-5.645905052624261
2215	94-95	-4.5799304256733215
2215	96-97	-3.1950669590449756
2215	98-99	-2.973502036780733
2215	100-101	-2.9738143476222447
2215	102-103	-2.8903179970815103
2215	104-105	-3.059303505789721
2215	106-107	-3.0563584938469432
2215	108-109	-5.137835011858865
2215	110-111	-4.551165773989386
2215	112-113	-3.4951722368182665
2215	114-115	-3.1963301871086855
2215	116-117	-3.08668973335401
2215	118-119	-3.244788184406609
2215	120-121	-3.2103082144015183
2215	122-123	-2.974657170816293
2215	124-125	-3.181937584669061
2216	1	-3.3110901790779543
2216	2	-3.226945439161149
2216	3	-4.836261649291057
2216	4	-4.486884831398921
2216	5	-4.474623058563353
2216	6	-3.53233459556931
2216	7	-3.415190646460786
2216	8	-3.271028642985712
2216	9	-3.9149653991897893
2216	10-11	-9.926618928319026
2216	12-13	-5.096517913417138
2216	14-15	-4.525614516463293
2216	16-17	-4.5239079172632515
2216	18-19	-4.161441982861856
2216	20-21	-3.625198708930373
2216	22-23	-3.828895240662046
2216	24-25	-4.26293656886871
2216	26-27	-4.210155252681879
2216	28-29	-4.567953178288793
2216	30-31	-4.322421703104439
2216	32-33	-4.632036478103597
2216	34-35	-4.146792546748749
2216	36-37	-3.8404082986838723
2216	38-39	-4.401592195025511
2216	40-41	-4.360535132268247
2216	42-43	-4.414260305298015
2216	44-45	-4.285641625541409
2216	46-47	-4.572753196569504
2216	48-49	-4.355139822051367
2216	50-51	-4.245768214947752
2216	52-53	-4.592907757730153
2216	54-55	-4.999597365444721
2216	56-57	-4.113835025741409
2216	58-59	-5.061715131886867
2216	60-61	-4.634871399323707
2216	62-63	-4.762670715900363
2216	64-65	-4.60011924252294
2216	66-67	-5.507630931950509
2216	68-69	-5.10378437887665
2216	70-71	-7.155137232591581
2216	72-73	-7.432373962078181
2216	74-75	-5.42519413140414
2216	76-77	-4.910765739417826
2216	78-79	-5.347995307439064
2216	80-81	-5.133137061124842
2216	82-83	-5.397089930333845
2216	84-85	-5.2091823986226125
2216	86-87	-5.485379232011308
2216	88-89	-5.674034749742674
2216	90-91	-5.814603421347716
2216	92-93	-5.599298688813757
2216	94-95	-8.635691053962091
2216	96-97	-10.580441595788644
2216	98-99	-7.48215867721407
2216	100-101	-6.588139681889775
2216	102-103	-7.42695620760168
2216	104-105	-10.091341510827423
2216	106-107	-6.859432228864698
2216	108-109	-9.219648276848275
2216	110-111	-6.59437291896106
2216	112-113	-6.077022224372545
2216	114-115	-6.483873913593698
2216	116-117	-5.948865798846171
2216	118-119	-6.344645903674124
2216	120-121	-7.075485952639799
2216	122-123	-9.632411778038502
2216	124-125	-8.135368250725222
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	149574.0
3	23867.0
4	15738.0
5	14082.0
6	14300.0
7	14237.0
8	15041.0
9	16277.0
10	17430.0
11	18037.0
12	18043.0
13	18578.0
14	19368.0
15	16817.0
16	13820.0
17	12273.0
18	11549.0
19	10872.0
20	11057.0
21	12103.0
22	14866.0
23	21260.0
24	27028.0
25	33310.0
26	40367.0
27	45078.0
28	52252.0
29	59662.0
30	67170.0
31	75988.0
32	90690.0
33	111869.0
34	138506.0
35	175766.0
36	229331.0
37	239696.0
38	68081.0
39	199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.17368857405167	19.15254781124533	13.873670874808388	22.800092739894612
2	14.922534042147149	21.148462498429062	39.9496472566909	23.979356202732887
3	18.637498959072005	26.7181001473592	28.774198019935127	25.87020287363367
4	11.097259824728926	17.454017465035275	37.62255371051823	33.826168999717574
5	13.74142861281125	39.12302863267493	32.78397828237056	14.351564472143249
6	30.88497382537323	37.15525108253086	17.699166289665868	14.260608802430038
7	28.093270945893543	33.94721195357134	20.27665899749244	17.68285810304268
8	25.493719957624645	35.99764857096916	19.74853979255929	18.76009167884691
9	25.967542123473947	17.67567561977916	19.958626238058898	36.39815601868799
10-11	24.128428619653604	28.079533240812715	26.844551134886522	20.947487004647154
12-13	25.203642755193606	25.377781902609787	27.462022749252814	21.956552592943797
14-15	22.54388012764282	26.617116268151264	25.299141852041156	25.53986175216476
16-17	22.178166878252977	30.1728974741507	25.471208317281214	22.177727330315104
18-19	22.157021626683616	28.79102996544219	27.5777318018203	21.4742166060539
20-21	23.11071282876413	27.783015156127373	27.423784496807873	21.682487518300626
22-23	22.613086633802236	27.85295240793948	27.17724685991475	22.356714098343534
24-25	22.479040643583517	27.899400260041578	27.30739070846464	22.314168387910264
26-27	22.603166642322464	28.0784409023987	26.9542040285564	22.36418842672244
28-29	22.320906690698386	28.059358552678347	27.364956655785694	22.25477810083757
30-31	22.335700266990944	27.974177777502028	27.393069196359328	22.297052759147697
32-33	22.19760242657945	28.132360571341263	27.085635784032775	22.58440121804651
34-35	22.6242362048901	27.838895176013196	27.139305006886534	22.397563612210174
36-37	22.837965627082646	27.61014073962626	27.07871564556632	22.47317798772477
38-39	22.649034495399654	27.671188891856197	27.162616294746673	22.51716031799748
40-41	22.53548333543224	27.730000049132464	27.343870608883098	22.3906460065522
42-43	22.58617208278146	27.787664066355884	27.03585512546825	22.590308725394408
44-45	22.518780121565477	27.985292679000583	26.84031733526988	22.655609864164063
46-47	22.560774471878137	28.022824874180372	26.588295026466845	22.828105627474642
48-49	22.76873002064345	27.9334962920641	26.530103630894597	22.767670056397854
50-51	22.80608183554349	28.193342411291482	26.297803735647857	22.702772017517166
52-53	22.840174056519505	28.007292772714358	26.39677099354462	22.755762177221513
54-55	22.494766719932326	28.0237267882668	26.41533823522188	23.06616825657899
56-57	22.579686128622374	28.415894440358365	26.263596633528476	22.740822797490786
58-59	22.754533902303763	27.77704667117396	26.351302612205114	23.117116814317164
60-61	22.784339117092024	27.725788709646555	26.66982307397526	22.820049099286162
62-63	22.799912618259423	27.97125709491906	26.412449571299497	22.816380715522023
64-65	22.622291794043853	27.866539300251674	26.40303197560563	23.108136930098848
66-67	22.473924300651618	28.124915170763675	26.343895461942857	23.05726506664185
68-69	22.44136788533498	27.968652326127902	26.491803990691043	23.098175797846075
70-71	22.178256932224087	27.925292441994383	26.618148013431558	23.27830261234997
72-73	22.530683719985173	27.840302476153845	26.33810695962969	23.290906844231294
74-75	22.36168277788951	28.126358794700952	26.226566688674506	23.285391738735026
76-77	22.548670342579648	27.850780131869584	26.539710821744418	23.06083870380635
78-79	22.54196338859502	27.795305437205787	26.625258756345772	23.037472417853426
80-81	22.336422674146714	28.37874623828313	26.328282552794587	22.95654853477557
82-83	22.349278126963927	28.187263278200902	26.277615873632215	23.185842721202956
84-85	22.503260497250068	27.90341948284442	26.1695717083196	23.42374831158591
86-87	22.648579576226645	27.693345331877723	26.329239058415865	23.328836033479767
88-89	22.599821466472225	27.536586317961852	26.507446264794744	23.356145950771175
90-91	22.32620929131155	27.8684023849754	26.66410045354241	23.141287870170643
92-93	22.309053875924604	27.760964377680295	26.501437203178575	23.42854454321653
94-95	22.20051268741062	27.83474879040722	26.378739293591615	23.58599922859054
96-97	22.410874608421135	27.661379091769696	26.330282391760342	23.59746390804883
98-99	22.285371275502015	27.49875719733328	26.34829547966237	23.867576047502332
100-101	22.265523210363696	27.718785028560553	26.479730484309272	23.535961276766475
102-103	22.320409539691802	27.327540499898795	26.751945212717693	23.60010474769171
104-105	22.207538355784394	27.374242895451644	26.804493366537486	23.613725382226473
106-107	22.12827977786774	27.554761413593376	26.788564355744576	23.52839445279431
108-109	22.144844168343326	27.62020820984053	26.394840743074038	23.840106878742105
110-111	21.934714332874645	27.618431124476867	26.73565718548981	23.71119735715868
112-113	22.067434901471625	27.62568911696084	26.51407963703814	23.792796344529396
114-115	21.769105807073384	27.819028149177612	26.50060182284073	23.911264220908276
116-117	21.59006859895014	27.751875257714637	26.849424852028175	23.80863129130705
118-119	21.79374193583412	27.895402323513846	26.399225684964527	23.9116300556875
120-121	21.689204246678827	27.884716309838165	26.51059006871148	23.915489374771525
122-123	21.682091743878225	27.76669010589014	26.542826563534085	24.00839158669755
124-125	21.78421953974498	27.9145902097714	26.219135726629077	24.08205452385454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3643.0
1	3786.0
2	4661.5
3	4892.5
4	3605.0
5	2318.5
6	1665.0
7	1631.5
8	2004.0
9	2100.0
10	1673.5
11	1329.0
12	1289.5
13	1355.0
14	1410.5
15	1514.5
16	1653.5
17	1786.0
18	1984.5
19	2223.0
20	2502.0
21	3032.5
22	3783.0
23	4672.5
24	5973.5
25	7618.0
26	9654.0
27	12206.5
28	15152.5
29	17972.5
30	20743.0
31	24233.5
32	28120.5
33	31340.0
34	34149.0
35	36595.0
36	39266.5
37	42878.5
38	45980.0
39	48099.5
40	50956.0
41	55148.5
42	58681.0
43	62244.5
44	65809.5
45	68425.0
46	70232.5
47	72931.0
48	78149.0
49	79290.5
50	77024.0
51	74289.5
52	71385.5
53	70215.0
54	67663.0
55	62843.0
56	56773.5
57	50798.0
58	46173.0
59	41550.0
60	35977.5
61	30039.0
62	24071.5
63	18695.5
64	14704.0
65	11837.5
66	9310.5
67	7290.5
68	5424.0
69	3949.5
70	3025.0
71	2382.5
72	1927.0
73	1566.5
74	1232.0
75	946.5
76	699.0
77	508.0
78	380.5
79	264.0
80	168.0
81	115.0
82	99.0
83	77.5
84	54.5
85	40.0
86	30.5
87	21.5
88	17.5
89	13.0
90	13.0
91	10.0
92	6.0
93	6.0
94	4.0
95	2.0
96	1.0
97	2.5
98	4.5
99	2.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09802593551175638
2	0.03220999885222797
3	0.04192987009495487
4	0.04901296775587819
5	0.05201165143714501
6	0.002946982238486347
7	0.0016544461689747916
8	0.002016356268438027
9	0.005480352934728996
10-11	0.06615199603760143
12-13	0.004989189228314605
14-15	0.009900826292458517
16-17	0.01949144392823426
18-19	0.016596163132528374
20-21	0.13204548486130055
22-23	0.014347150371578271
24-25	0.013468225844310412
26-27	0.005919815198362925
28-29	0.0036966531588030495
30-31	0.001757849054535716
32-33	0.005247696442216916
34-35	0.01734583405284508
36-37	0.002223162039559876
38-39	0.007315754153435406
40-41	0.03275286400142283
42-43	0.01274440564538394
44-45	0.1147255015298457
46-47	0.003308892337949583
48-49	0.007987872909581414
50-51	0.010004229178019443
52-53	0.010986556590848224
54-55	0.0085565887801665
56-57	0.0056613079844606146
58-59	0.014450553257139195
60-61	0.028384092086473765
62-63	0.007212351267874481
64-65	0.006695336840069859
66-67	0.007729365695679103
68-69	0.006772889004240553
70-71	0.004161966143827209
72-73	6.72118756146009E-4
74-75	0.0011891331839506314
76-77	0.0077552164170693345
78-79	0.0213526958683309
80-81	0.0030245344026570403
82-83	0.004213667586607672
84-85	0.0023007142037305695
86-87	0.0030245344026570403
88-89	0.004730682014412294
90-91	0.0038259067657542053
92-93	0.002249012760950107
94-95	0.003102086566827734
96-97	0.0012925360695115557
98-99	0.0023524156465110314
100-101	0.002378266367901263
102-103	5.428651491948534E-4
104-105	0.004472174800509983
106-107	9.823274128287825E-4
108-109	0.0014217896764627113
110-111	0.002662624303193805
112-113	0.004885786342753681
114-115	0.0029986836812668093
116-117	0.005919815198362925
118-119	0.004420473357729521
120-121	0.007910320745410721
122-123	0.005273547163607148
124-125	0.05694913922267915
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1934182.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.25599535683984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.36468287706211	42.196065624814906
2	11.40483307287214	12.60370806640313
3	4.63243082162939	7.67908727912506
4	2.425697997169259	5.361374290747211
5	1.413014729104155	3.9038767655262747
6	0.9157482469778053	3.03603485298239
7	0.6365055646347232	2.4619523966841292
8	0.4180248450823836	1.8478703119132702
9	0.3092999210407748	1.5381607500809982
>10	1.3862000914275907	12.969488197936785
>50	0.06138055126206432	2.331947258977869
>100	0.03021960250059819	2.770187514516589
>500	0.0011770075421825737	0.4604600008635047
>1k	7.84671694788382E-4	0.8397866894279664
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4238	0.21911071450359892	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2903	0.15008928839168187	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2021	0.10448861585931417	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.170144278046223E-5	0.0
4	0.0	0.0	0.0	5.170144278046223E-5	0.0
5	0.0	0.0	0.0	5.170144278046223E-5	0.0
6	0.0	0.0	0.0	5.170144278046223E-5	0.0
7	0.0	0.0	0.0	5.170144278046223E-5	0.0
8	0.0	0.0	0.0	5.170144278046223E-5	0.0
9	0.0	0.0	0.0	5.170144278046223E-5	0.0
10-11	0.0	0.0	0.0	5.170144278046223E-5	0.0
12-13	1.0340288556092446E-4	0.0	0.0	5.170144278046223E-5	0.0
14-15	1.0340288556092446E-4	0.0	0.0	5.170144278046223E-5	0.0
16-17	1.0340288556092446E-4	0.0	0.0	1.0340288556092446E-4	0.0
18-19	1.809550497316178E-4	0.0	0.0	1.0340288556092446E-4	0.0
20-21	2.5850721390231117E-4	0.0	0.0	2.0680577112184895E-4	0.0
22-23	2.5850721390231117E-4	0.0	0.0	6.721187561460091E-4	0.0
24-25	2.5850721390231117E-4	0.0	0.0	0.001137431741170169	0.0
26-27	2.5850721390231117E-4	0.0	0.0	0.0019388041042673336	0.0
28-29	3.3605937807300453E-4	0.0	0.0	0.0029211315170961163	0.0
30-31	3.619100994632356E-4	5.170144278046223E-5	0.0	0.006669486118679627	0.0
32-33	3.619100994632356E-4	5.170144278046223E-5	0.0	0.012098137610628161	0.0
34-35	5.170144278046223E-4	5.170144278046223E-5	0.0	0.020137711962990038	0.0
36-37	6.72118756146009E-4	5.170144278046223E-5	0.0	0.03474336954847062	0.0
38-39	6.72118756146009E-4	5.170144278046223E-5	0.0	0.05736275076492285	0.0
40-41	7.238201989264712E-4	5.170144278046223E-5	0.0	0.08758224407010301	0.0
42-43	8.013723630971646E-4	5.170144278046223E-5	0.0	0.11650920130577164	0.0
44-45	8.272230844873957E-4	5.170144278046223E-5	0.0	0.1487192001579996	0.0
46-47	0.0010857302983897069	5.170144278046223E-5	0.0	0.1904164137604424	0.0
48-49	0.0011891331839506314	5.170144278046223E-5	0.0	0.2367667572131268	0.0
50-51	0.0011891331839506314	5.170144278046223E-5	0.0	0.2882872449438574	0.0
52-53	0.0011891331839506314	5.170144278046223E-5	0.0	0.3420825961569284	0.0
54-55	0.0011891331839506314	5.170144278046223E-5	0.0	0.3989541832154368	0.0
56-57	0.0011891331839506314	5.170144278046223E-5	0.0	0.46469256771079454	0.0
58-59	0.0011891331839506314	5.170144278046223E-5	0.0	0.5427617463092924	0.0
60-61	0.0011891331839506314	5.170144278046223E-5	0.0	0.6329807639611991	0.0
62-63	0.0012149839053408624	5.170144278046223E-5	0.0	0.7247249741751294	0.0
64-65	0.0012408346267310936	5.170144278046223E-5	0.0	0.8173739596377176	0.0
66-67	0.0014476403978529425	5.170144278046223E-5	0.0	0.9233877680590554	0.0
68-69	0.0014476403978529425	5.170144278046223E-5	0.0	1.0500563028711878	0.0
70-71	0.0015510432834138668	5.170144278046223E-5	0.0	1.1927522849452636	0.0
72-73	0.0016544461689747916	5.170144278046223E-5	0.0	1.3459178091823831	0.0
74-75	0.001731998333145485	5.170144278046223E-5	0.0	1.4994193927975754	0.0
76-77	0.001809550497316178	5.170144278046223E-5	0.0	1.6673973803912974	0.0
78-79	0.0018354012187064093	5.170144278046223E-5	0.0	1.8607607763902259	0.0
80-81	0.0019646548256575646	5.170144278046223E-5	0.0	2.0865151262911144	0.0
82-83	0.0019646548256575646	5.170144278046223E-5	0.0	2.3293826537523357	0.0
84-85	0.0020680577112184894	5.170144278046223E-5	0.0	2.5743440896461656	0.0
86-87	0.0021714605967794137	5.170144278046223E-5	0.0	2.8263110710367485	0.0
88-89	0.0021714605967794137	5.170144278046223E-5	0.0	3.1113411250854366	0.0
90-91	0.002249012760950107	5.170144278046223E-5	0.0	3.4283226707724506	0.0
92-93	0.0023265649251208004	5.170144278046223E-5	0.0	3.773429801332036	0.0
94-95	0.0023265649251208004	5.170144278046223E-5	0.0	4.126912565622057	0.0
96-97	0.0023265649251208004	5.170144278046223E-5	0.0	4.471476831032447	0.0
98-99	0.0024299678106817248	5.170144278046223E-5	0.0	4.844528591414872	0.0
100-101	0.002507519974852418	5.170144278046223E-5	0.0	5.277373070372901	0.0
102-103	0.002662624303193805	5.170144278046223E-5	0.0	5.740592146964453	0.0
104-105	0.002895280795705885	5.170144278046223E-5	0.0	6.204560894476321	0.0
106-107	0.002895280795705885	7.755216417069335E-5	0.0	6.660955380620852	0.0
108-109	0.002895280795705885	1.2925360695115559E-4	0.0	7.138676711912323	0.0
110-111	0.002895280795705885	1.551043283413867E-4	0.0	7.689374629688416	0.0
112-113	0.002895280795705885	1.551043283413867E-4	0.0	8.267681117909277	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	10950	0.0	31.743145	1
TACATGG	11025	0.0	30.220621	2
GTATCAA	10340	0.0	29.989443	1
ACATGGG	11285	0.0	26.784191	3
GAGTACT	3630	0.0	26.05487	12-13
CGCATAG	275	7.4578566E-11	25.989822	8
TATCAAC	12295	0.0	25.405323	2
ATCAACG	11860	0.0	24.43209	3
CAACGCA	12040	0.0	24.250212	5
TCAACGC	11950	0.0	24.18479	4
AACGCAG	11715	0.0	24.097296	6
CATGGGG	4025	0.0	23.83573	4
GTACTTT	4280	0.0	22.483767	14-15
ACGCATA	345	5.2750693E-11	22.4429	8
GGTATCA	7835	0.0	22.333641	1
CATGGGA	8200	0.0	22.30966	4
ATGGGAG	2885	0.0	22.099575	5
ACGCAGA	13020	0.0	21.407557	7
CGCAGAG	12780	0.0	21.204853	8
AGTACTT	4200	0.0	20.040363	12-13
>>END_MODULE