FastQCFastQC Report
Thu 26 May 2016
SRR1364489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364489_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934182
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104040.5379018106879291No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93590.483873802982346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59830.3093297321555055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23150.11968884003677006No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22030.1138982784453583No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87100.038.2958531
GTATCAA123450.037.363411
TATCAAC153450.029.8737472
ATCAACG152150.029.1886883
TCAACGC152750.028.7228074
CAACGCA152550.028.5089345
AACGCAG153950.027.8632246
TCGACCG755.3174826E-627.75398818-19
GAGTACT92450.027.49953512-13
GTACATG99550.026.9130671
TACATGG99750.025.577682
CGCAGAG174850.024.93078
GTACTTT105400.024.62892214-15
AGTACTT98500.024.60296812-13
CAACGCG1951.4668785E-624.4079446
ACGCAGA174900.024.3215927
GCAGAGT180750.023.360189
ACATGGG103500.022.923083
CATGGGG40500.022.0783444
GTACCGT1903.3095526E-521.9189766
Produced by FastQC (version 0.11.5)