##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364489_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1934182 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.98420934534599 31.0 28.0 33.0 25.0 34.0 2 29.12199420737035 31.0 28.0 33.0 25.0 34.0 3 29.192147378064732 31.0 28.0 34.0 25.0 34.0 4 32.61116999330984 35.0 32.0 37.0 26.0 37.0 5 31.770924866429322 35.0 32.0 37.0 20.0 37.0 6 31.8151311510499 35.0 32.0 37.0 21.0 37.0 7 31.249260410861027 35.0 31.0 37.0 17.0 37.0 8 31.37413697366639 35.0 32.0 37.0 17.0 37.0 9 31.959014198250216 37.0 30.0 39.0 11.0 39.0 10-11 32.128888336257916 37.0 31.0 39.0 15.5 39.0 12-13 31.89846276100181 37.0 30.5 39.0 13.0 39.0 14-15 32.79497947969736 37.5 31.0 40.0 12.0 41.0 16-17 32.85361408595468 38.0 31.0 40.0 13.0 41.0 18-19 32.7555710889668 38.0 31.0 40.0 11.0 41.0 20-21 32.66506486980026 38.0 31.0 40.0 10.5 41.0 22-23 32.62873788505942 37.5 31.0 40.0 10.5 41.0 24-25 32.46803144688556 37.0 30.5 40.0 10.0 41.0 26-27 32.47202331528264 37.0 30.5 40.0 10.0 41.0 28-29 32.31672433100918 37.0 30.0 40.0 9.5 41.0 30-31 31.779230703212 37.0 30.0 40.0 8.0 41.0 32-33 31.670137556858663 36.5 30.0 40.0 8.0 41.0 34-35 31.489019130567858 36.0 30.0 40.0 2.0 41.0 36-37 31.389022594564523 36.0 30.0 40.0 2.0 41.0 38-39 31.16307436425321 36.0 30.0 40.0 2.0 41.0 40-41 30.82025709059437 36.0 28.5 40.0 2.0 41.0 42-43 30.69638353577895 36.0 28.5 40.0 2.0 41.0 44-45 30.584188302858777 36.0 28.0 40.0 2.0 41.0 46-47 30.352559893536387 36.0 28.0 40.0 2.0 41.0 48-49 30.018079736033116 35.0 27.0 40.0 2.0 41.0 50-51 29.689593585298592 35.0 26.5 40.0 2.0 41.0 52-53 29.560370482198678 35.0 26.0 40.0 2.0 41.0 54-55 29.089054184146065 35.0 25.5 39.0 2.0 41.0 56-57 29.10189087686681 35.0 26.0 39.0 2.0 41.0 58-59 28.87913288408226 35.0 26.0 39.0 2.0 41.0 60-61 28.598675564140294 34.0 25.0 39.0 2.0 40.5 62-63 28.32619655234099 34.0 24.5 38.5 2.0 40.0 64-65 27.84970752493819 34.0 23.0 38.0 2.0 40.0 66-67 27.49398117653871 34.0 22.0 37.5 2.0 40.0 68-69 27.292426979467287 33.5 22.0 37.0 2.0 40.0 70-71 27.34172714873781 33.5 23.5 37.0 2.0 39.5 72-73 27.13282048948858 33.5 22.5 36.0 2.0 39.0 74-75 26.77975469733458 33.0 22.0 36.0 2.0 39.0 76-77 26.657727142533638 33.0 22.0 35.5 2.0 38.5 78-79 26.2654781194324 33.0 20.5 35.0 2.0 37.0 80-81 26.067897695253084 33.0 20.0 35.0 2.0 37.0 82-83 25.915046257280856 33.0 20.0 35.0 2.0 36.5 84-85 25.524176370165787 33.0 18.5 35.0 2.0 36.0 86-87 25.3867443704884 32.0 18.5 35.0 2.0 36.0 88-89 25.31818489676773 32.5 19.0 35.0 2.0 35.5 90-91 25.151051969256255 32.0 18.0 35.0 2.0 35.0 92-93 25.172282649719623 32.0 18.5 35.0 2.0 35.0 94-95 25.160318935860225 32.5 18.5 35.0 2.0 35.0 96-97 25.030372271068597 32.0 18.0 35.0 2.0 35.0 98-99 24.911724956596636 32.0 17.0 35.0 2.0 35.0 100-101 24.817516397112577 32.0 15.5 35.0 2.0 35.0 102-103 23.65409847677209 30.0 10.5 34.0 2.0 35.0 104-105 24.57696380175185 32.0 15.0 35.0 2.0 35.0 106-107 24.702951687069778 32.0 10.0 35.0 2.0 35.0 108-109 24.752197052810956 33.0 10.0 35.0 2.0 35.0 110-111 24.68027569277348 33.0 7.0 35.0 2.0 35.0 112-113 24.60409826996632 33.0 7.0 35.0 2.0 35.0 114-115 24.529507047423664 33.0 2.0 35.0 2.0 35.0 116-117 24.43412176310192 33.0 2.0 35.0 2.0 35.0 118-119 24.302454991308984 33.0 2.0 35.0 2.0 35.0 120-121 24.127781149860766 32.5 2.0 35.0 2.0 35.0 122-123 23.824208890373296 32.0 2.0 35.0 2.0 35.0 124-125 23.042285059006858 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8933877682156073 1101 2 0.9084968067745045 1101 3 0.8914847191523592 1101 4 0.8337888974003818 1101 5 0.9134237690596727 1101 6 0.9395805559792834 1101 7 1.4089362184365122 1101 8 1.2562288303735052 1101 9 1.9422707769504015 1101 10-11 1.6098887708506027 1101 12-13 1.856998164052552 1101 14-15 1.829224755614355 1101 16-17 1.693459734795006 1101 18-19 1.8082485610802408 1101 20-21 1.9925529340454133 1101 22-23 2.0819688531599496 1101 24-25 2.3181269305815135 1101 26-27 1.94913541791108 1101 28-29 2.0591875150301036 1101 30-31 2.525169155729845 1101 32-33 2.367744686014536 1101 34-35 2.4490689943592017 1101 36-37 2.2780083899086776 1101 38-39 2.334107803933037 1101 40-41 2.5999217343581265 1101 42-43 2.5229269412212716 1101 44-45 2.507399494539815 1101 46-47 2.397651731032031 1101 48-49 2.394210956380011 1101 50-51 2.6186100150873415 1101 52-53 2.4702053743440686 1101 54-55 2.831227427061865 1101 56-57 2.5917700083288437 1101 58-59 2.631302307109415 1101 60-61 2.7159220836842373 1101 62-63 2.6640654389243323 1101 64-65 2.8725074176323275 1101 66-67 3.0472363752588123 1101 68-69 3.039652075301298 1101 70-71 2.595121372357557 1101 72-73 2.9152196683786045 1101 74-75 2.9495556646082406 1101 76-77 2.705846577684369 1101 78-79 2.7652342913432513 1101 80-81 2.804630556254679 1101 82-83 2.611727702741888 1101 84-85 2.901812796853733 1101 86-87 2.7439956644603427 1101 88-89 2.4759709942596473 1101 90-91 2.5040467868149108 1101 92-93 2.489391574843353 1101 94-95 2.4186122628430766 1101 96-97 2.278326334033835 1101 98-99 2.4415826075555884 1101 100-101 2.5511928930359176 1101 102-103 2.3060982110048904 1101 104-105 2.3131757570568325 1101 106-107 2.456739131217727 1101 108-109 2.4083726271136285 1101 110-111 2.3712625148137576 1101 112-113 2.2575331819503717 1101 114-115 2.276473565123201 1101 116-117 2.326751891687543 1101 118-119 2.3284035873926463 1101 120-121 2.287461087254595 1101 122-123 2.2251559551405506 1101 124-125 2.117939584509184 1102 1 0.49700285923998067 1102 2 -1.7628440538013237 1102 3 -0.06441780956066268 1102 4 0.4023944486042126 1102 5 0.9252135442227463 1102 6 0.40082545968970607 1102 7 1.232287602527446 1102 8 1.0759814949821909 1102 9 1.8427814116043422 1102 10-11 1.4721581577816494 1102 12-13 1.5177851499060502 1102 14-15 1.5788220728384417 1102 16-17 1.4956537047828888 1102 18-19 1.6862169411398682 1102 20-21 1.9343426649902256 1102 22-23 1.80286571384665 1102 24-25 2.1322331387500952 1102 26-27 1.7099424961117222 1102 28-29 1.7020964641270098 1102 30-31 2.1256160621608764 1102 32-33 1.7702073951898392 1102 34-35 2.08761155179468 1102 36-37 1.4907466036655848 1102 38-39 1.3428128699546846 1102 40-41 1.559250585253828 1102 42-43 1.3265198799745548 1102 44-45 1.4698304722584474 1102 46-47 0.9420952371865106 1102 48-49 1.1567689160070067 1102 50-51 1.3434139906758418 1102 52-53 1.188926319035975 1102 54-55 1.3329268103896617 1102 56-57 0.9870726483640375 1102 58-59 0.9826722697730261 1102 60-61 1.0496281446167863 1102 62-63 1.3207134336207282 1102 64-65 1.3309529817694639 1102 66-67 1.4420555263393489 1102 68-69 1.4205219283359725 1102 70-71 0.9185607615760922 1102 72-73 1.1324253826291901 1102 74-75 1.3707761921925083 1102 76-77 1.2178663417378743 1102 78-79 1.1949874337248723 1102 80-81 1.2408027344042374 1102 82-83 0.9568625668251904 1102 84-85 0.9412722577123205 1102 86-87 0.9483089510668385 1102 88-89 1.1029981993839613 1102 90-91 0.940844383757252 1102 92-93 1.0125762884389715 1102 94-95 0.9199660966257639 1102 96-97 1.1245018371810431 1102 98-99 0.9437586853245108 1102 100-101 0.7235715346659894 1102 102-103 0.4768753965815016 1102 104-105 0.7588290856620468 1102 106-107 1.1145958483007625 1102 108-109 0.9537528594148128 1102 110-111 0.8078084990567405 1102 112-113 0.8888388814579429 1102 114-115 1.1531675964234758 1102 116-117 0.812524342135319 1102 118-119 0.40595319893520454 1102 120-121 0.7776881501902331 1102 122-123 1.0892734497510475 1102 124-125 1.1697588586459098 1103 1 0.6218311727544865 1103 2 0.4857041168563754 1103 3 0.47095637898805265 1103 4 0.6049048308504865 1103 5 0.9274427221903103 1103 6 0.7842149535326683 1103 7 0.978763910059083 1103 8 0.8045443881759731 1103 9 1.62960049697692 1103 10-11 1.3029714317840018 1103 12-13 1.1141677937808758 1103 14-15 0.9233828508675188 1103 16-17 1.051956633227995 1103 18-19 1.2787788074676953 1103 20-21 1.4384570080295802 1103 22-23 1.3380254209402764 1103 24-25 1.7187774190917509 1103 26-27 1.08285524812743 1103 28-29 1.208289602810865 1103 30-31 1.966722579560102 1103 32-33 1.7195919581990715 1103 34-35 1.7702135008775741 1103 36-37 1.7122936282180063 1103 38-39 1.7197341107858932 1103 40-41 1.8853478269433879 1103 42-43 1.7973477890566016 1103 44-45 1.7069318817781003 1103 46-47 1.6267630234371282 1103 48-49 1.6850427125164735 1103 50-51 1.782785917303773 1103 52-53 1.7184434360639251 1103 54-55 1.982024431128 1103 56-57 1.752469131892056 1103 58-59 1.9928071851141738 1103 60-61 1.8283225316872311 1103 62-63 1.9357860405526957 1103 64-65 1.9573190878549127 1103 66-67 2.301884163015874 1103 68-69 2.3543001124896854 1103 70-71 2.0349573195486883 1103 72-73 2.070706184181901 1103 74-75 2.0980167341576 1103 76-77 1.9309756381171148 1103 78-79 2.1571511345890464 1103 80-81 2.129174629249235 1103 82-83 2.0360968797624857 1103 84-85 2.300910676748291 1103 86-87 2.0890352358278186 1103 88-89 2.0148949556265876 1103 90-91 2.0355647163645543 1103 92-93 1.8025187400035954 1103 94-95 1.7495596739993893 1103 96-97 1.7303532880291677 1103 98-99 1.8055312534983656 1103 100-101 1.6690039222402682 1103 102-103 1.5585999767961773 1103 104-105 1.6519154130078917 1103 106-107 1.5908806626084555 1103 108-109 1.5788679125573637 1103 110-111 1.571683881385134 1103 112-113 1.5092426483964871 1103 114-115 1.3612579094341477 1103 116-117 1.4193839944727173 1103 118-119 1.5553492379288087 1103 120-121 1.6312716009675405 1103 122-123 1.717227689156715 1103 124-125 1.5029897151361666 1104 1 0.5619582564620806 1104 2 0.48220307696459486 1104 3 0.466082791307727 1104 4 0.3334073036440799 1104 5 0.7865937395100921 1104 6 0.8235535260546101 1104 7 1.1101017664339174 1104 8 0.9683063316404521 1104 9 1.4468653132323226 1104 10-11 0.9801799689218811 1104 12-13 1.1003447469273162 1104 14-15 1.1085239932153215 1104 16-17 0.8923955730791207 1104 18-19 0.8453972482963437 1104 20-21 1.232049121743053 1104 22-23 1.1617943962564894 1104 24-25 1.4414675763018465 1104 26-27 1.1645874613602345 1104 28-29 1.1401867162073742 1104 30-31 1.5601246388476078 1104 32-33 1.5416497044460264 1104 34-35 1.4087720178535577 1104 36-37 1.219713274830994 1104 38-39 1.5005770945188601 1104 40-41 1.4333997589507206 1104 42-43 1.284875720515494 1104 44-45 1.076159996304117 1104 46-47 1.2954006811499958 1104 48-49 0.971640594495188 1104 50-51 1.386661273681142 1104 52-53 1.264451280467192 1104 54-55 1.5635895062373741 1104 56-57 1.4688219643693259 1104 58-59 1.4622450602635695 1104 60-61 1.5265241437068404 1104 62-63 1.6460232683342575 1104 64-65 1.7043210319431843 1104 66-67 1.5990725564909134 1104 68-69 1.7371086425795106 1104 70-71 1.5346527139895976 1104 72-73 1.6878689960557693 1104 74-75 1.770427885637499 1104 76-77 1.4860314220480788 1104 78-79 1.5685710683899963 1104 80-81 1.5493529348929655 1104 82-83 1.5287328783472063 1104 84-85 1.8279803186119494 1104 86-87 1.75910407384875 1104 88-89 1.5973258983666163 1104 90-91 1.5691263654715115 1104 92-93 1.4303657842663888 1104 94-95 1.3823568623928644 1104 96-97 1.4632320353712096 1104 98-99 1.549891797519031 1104 100-101 1.5990497062380662 1104 102-103 1.526652848544071 1104 104-105 1.4050617210933254 1104 106-107 1.6007930014221934 1104 108-109 1.3763537723987085 1104 110-111 1.4079861906557767 1104 112-113 1.3648299426991244 1104 114-115 1.203455116014844 1104 116-117 1.2037323724987488 1104 118-119 1.1766129844192115 1104 120-121 1.22213160129564 1104 122-123 1.323866267572292 1104 124-125 1.1805459461527512 1105 1 0.40282176351522025 1105 2 0.5315497903398239 1105 3 0.457522480263826 1105 4 0.6243731215450694 1105 5 0.7748233375400098 1105 6 0.7976885743165525 1105 7 1.1135337288714915 1105 8 0.9777366423335998 1105 9 1.635730120039014 1105 10-11 1.2308594954272962 1105 12-13 1.402332137589763 1105 14-15 1.2502474298106279 1105 16-17 1.176652632157321 1105 18-19 1.4468546057130354 1105 20-21 1.579344176806167 1105 22-23 1.4723996487790885 1105 24-25 1.733243785812732 1105 26-27 1.477435549937674 1105 28-29 1.5557311762444286 1105 30-31 1.8226698681832367 1105 32-33 1.6617126904457002 1105 34-35 1.759789566686635 1105 36-37 1.4964100904450675 1105 38-39 1.7030614382796045 1105 40-41 1.7920252273932888 1105 42-43 1.7588071551210476 1105 44-45 1.757142398509135 1105 46-47 1.7500436948254894 1105 48-49 1.8129376613167771 1105 50-51 1.8753848624080902 1105 52-53 1.7848164005375367 1105 54-55 2.0138949164358273 1105 56-57 1.8650427221001387 1105 58-59 1.707612065264577 1105 60-61 1.5017425256535546 1105 62-63 1.4820012819680919 1105 64-65 1.9556421549030993 1105 66-67 2.1355055661579065 1105 68-69 2.1404679644694014 1105 70-71 1.9442044317040263 1105 72-73 2.2093622972744775 1105 74-75 2.2409561074983166 1105 76-77 2.0062701771894638 1105 78-79 2.145784514441324 1105 80-81 2.1399910988707234 1105 82-83 1.9917009596050512 1105 84-85 2.208361384218545 1105 86-87 2.223296408573759 1105 88-89 2.058579952767616 1105 90-91 2.0275226798328916 1105 92-93 2.0116681155218927 1105 94-95 1.7798368463835565 1105 96-97 1.8525622786182971 1105 98-99 1.8431136691332952 1105 100-101 1.9875007437625385 1105 102-103 1.7899000024016267 1105 104-105 1.4098641026993626 1105 106-107 1.5716160747839005 1105 108-109 1.5733550508600018 1105 110-111 1.7145650975564521 1105 112-113 1.7102319924956504 1105 114-115 1.7150069894610631 1105 116-117 1.687764155361318 1105 118-119 1.8173139297029444 1105 120-121 1.7892296813613235 1105 122-123 1.9179558861765038 1105 124-125 1.6876741213351707 1106 1 0.21607289516419925 1106 2 0.16691143401319408 1106 3 0.1751751487660691 1106 4 0.18336934697146745 1106 5 0.29063169345389994 1106 6 0.4192372441691461 1106 7 0.6871283079770301 1106 8 0.5277547303495567 1106 9 1.1744745235477652 1106 10-11 0.7665638771818664 1106 12-13 0.724917255507048 1106 14-15 0.7765481545030042 1106 16-17 0.7386271256796846 1106 18-19 0.6688934729377038 1106 20-21 0.6150711487878482 1106 22-23 0.5607430599223804 1106 24-25 0.5704939196915007 1106 26-27 0.554270876577057 1106 28-29 0.5395270163279946 1106 30-31 0.9284149324033457 1106 32-33 0.6948799469037859 1106 34-35 0.6516171614513162 1106 36-37 0.6670755615890656 1106 38-39 0.8447937371212859 1106 40-41 1.0804173447552117 1106 42-43 1.0082689746287272 1106 44-45 0.9239509578406206 1106 46-47 0.8044589599920045 1106 48-49 0.7198338348384326 1106 50-51 1.0702905445152453 1106 52-53 0.6951702511103619 1106 54-55 1.2528459994102619 1106 56-57 0.9190467184591 1106 58-59 0.8847313215014054 1106 60-61 1.1506775178414514 1106 62-63 1.2606980222088815 1106 64-65 1.4179480913931037 1106 66-67 1.4500118804958468 1106 68-69 1.3442181955178114 1106 70-71 1.2326745064618443 1106 72-73 1.1069116587680412 1106 74-75 1.3120495051033068 1106 76-77 0.91798239711947 1106 78-79 0.8749658520002264 1106 80-81 1.097479036058914 1106 82-83 0.880507322617543 1106 84-85 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80-81 -5.263783803928231 2216 82-83 -5.392232580546445 2216 84-85 -5.246450220470358 2216 86-87 -5.748853790221986 2216 88-89 -5.420728673874777 2216 90-91 -4.987082650110512 2216 92-93 -4.581347558865438 2216 94-95 -4.299883668255294 2216 96-97 -4.594747821317284 2216 98-99 -4.438248144606476 2216 100-101 -4.787998329900457 2216 102-103 -4.566232663657235 2216 104-105 -4.359649374909587 2216 106-107 -4.478090641430928 2216 108-109 -4.586924393402377 2216 110-111 -4.3215478567476815 2216 112-113 -4.3636424172843675 2216 114-115 -4.282387495063929 2216 116-117 -4.163958025147824 2216 118-119 -4.350366669806828 2216 120-121 -4.258993858391907 2216 122-123 -4.245834910105387 2216 124-125 -4.113708177207691 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 95154.0 3 31945.0 4 15557.0 5 13179.0 6 14155.0 7 16884.0 8 18482.0 9 19289.0 10 20344.0 11 21953.0 12 23578.0 13 25482.0 14 26519.0 15 21671.0 16 15262.0 17 13505.0 18 12154.0 19 11342.0 20 11279.0 21 12137.0 22 14659.0 23 19266.0 24 26181.0 25 34315.0 26 41526.0 27 46476.0 28 52449.0 29 59220.0 30 66428.0 31 75270.0 32 88415.0 33 104541.0 34 126883.0 35 161421.0 36 219068.0 37 268379.0 38 89438.0 39 376.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62164763846767 19.641655712082475 14.458860792249329 23.277835857200525 2 15.921345357741304 21.23456126300747 38.37192769844746 24.472165680803773 3 18.91828173357006 25.959449524398426 29.868750717357518 25.253518024673998 4 12.958307046686121 18.010730131697397 36.0692164973444 32.96174632427208 5 13.281221725773479 38.61017215546417 33.330007207181126 14.778598911581225 6 30.936107290944996 37.50286943304139 16.895531956694825 14.665491319318782 7 27.091265028304544 33.84416853941301 20.422563985859366 18.642002446423074 8 26.096613452094996 35.56087276171529 19.13346313842234 19.209050647767377 9 26.08808271403622 16.26212011072381 19.621473056827124 38.02832411841285 10-11 24.888937948206628 27.320941604561966 26.416107403369875 21.374013043861527 12-13 26.166517894419012 24.52741222372761 27.46661413752553 21.83945574432785 14-15 22.999408273775103 25.464985912572903 26.153652868941556 25.381952944710434 16-17 21.915419567258574 28.934050570604853 26.674018969147216 22.476510892989356 18-19 21.811843104956115 26.70283765991873 29.973013936638203 21.512305298486957 20-21 22.545420891166945 25.804577777467557 30.120218426667222 21.529782904698273 22-23 22.113680910257983 25.686263560985722 30.068459614721426 22.131595914034868 24-25 22.06450075113601 25.882484635384284 29.978586613052627 22.07442800042708 26-27 22.106338876189778 26.075226425366882 29.924082807286766 21.894351891156575 28-29 21.8326564483314 25.978165611484382 30.3320140323302 21.857163907854023 30-31 21.761485914446173 25.870240995779824 30.19118093748586 22.177092152288136 32-33 21.673488675568624 26.008501789646367 30.021854211162246 22.296155323622763 34-35 22.147644587303528 25.884037522396774 29.882198990099795 22.086118900199907 36-37 22.401602974737276 25.645372602181627 29.778135556941177 22.174888866139923 38-39 22.18816509523343 25.73697689730755 29.878420623642665 22.196437383816352 40-41 22.05107577194276 25.788565082470505 29.943680570132763 22.216678575453976 42-43 22.27332678096601 25.90117885739605 29.51501969070827 22.31047467092967 44-45 22.248380624835683 26.074725597437435 29.12406519327676 22.552828584450122 46-47 22.3872131859496 26.226669699821116 28.588471600368116 22.79764551386117 48-49 22.66434733874441 26.263364183600192 28.246916912119392 22.825371565536006 50-51 22.57799981586861 26.5220062873629 27.918404966996 22.981588929772496 52-53 22.75484487882859 26.42603602609166 27.98400770558502 22.83511138949473 54-55 22.328148636713326 26.404418761409044 27.945771911314143 23.321660690563487 56-57 22.47292845729755 26.831621971165564 27.77393319806502 22.921516373471864 58-59 22.602067075918157 26.273594282646172 27.906610625734967 23.217728015700697 60-61 22.65872601440816 26.19329515009446 28.217639291442065 22.930339544055318 62-63 22.794515675223494 26.33772057945358 28.04300249151185 22.824761253811072 64-65 22.559290963189902 26.263322311539877 27.999131914131926 23.178254811138295 66-67 22.429539430998695 26.49957163408215 27.96529938678895 23.1055895481302 68-69 22.38742306674167 26.386478979848448 28.074615954137847 23.15148199927203 70-71 22.29657415258619 26.463611358137033 27.994772816578084 23.245041672698697 72-73 22.508998663587864 26.420319466138793 27.67242329340998 23.39825857686336 74-75 22.30615471636849 26.68856839429728 27.617608037665182 23.387668851669048 76-77 22.444228788637087 26.43476314167656 27.934438451579812 23.18656961810654 78-79 22.51365069221586 26.461325925431883 27.831772183247327 23.19325119910493 80-81 22.161958290270228 26.828878639338594 27.77599981801045 23.233163252380727 82-83 22.25605799670041 26.672221133617935 27.6747136640404 23.397007205641255 84-85 22.279082320071225 26.334982954034313 27.52569303198975 23.86024169390471 86-87 22.439311397043806 26.16412562035429 27.727632993257593 23.668929989344306 88-89 22.400680813926286 26.062899286019142 27.829949556796237 23.70647034325834 90-91 22.21168083979948 26.436399789884685 27.855246885495426 23.49667248482041 92-93 22.138874749662445 26.369492153741835 27.7808181782225 23.71081491837322 94-95 21.996981548044985 26.421174869603703 27.720510375863967 23.861333206487345 96-97 22.256245417855396 26.26578351699827 27.571429753202718 23.906541311943617 98-99 22.067700971263303 26.118715818883643 27.530604669053893 24.28297854079916 100-101 22.09517250721376 26.250484062516318 27.739328521919955 23.915014908349967 102-103 22.179033490999778 25.9853377187302 27.845202005122154 23.990426785147868 104-105 22.111155775516984 25.930040029935203 27.921274029917107 24.037530164630702 106-107 21.94567673492727 26.124138879489273 27.838947812781694 24.091236572801762 108-109 22.102857952354018 26.1418522145279 27.430872585930384 24.3244172471877 110-111 21.83188413889518 26.10899824111328 27.758432781264187 24.300684838727356 112-113 22.01352354988402 26.07904170317005 27.5165748405721 24.390859906373834 114-115 21.69042651396806 26.244939394229235 27.49526220536294 24.56937188643976 116-117 21.621388266460965 26.045377322299558 27.753050126616834 24.580184284622646 118-119 21.61512115437958 26.246552451976036 27.458031620371482 24.680294773272905 120-121 21.445071941571936 26.203028841257016 27.58043760039997 24.771461616771077 122-123 21.577216037390766 26.077033142165817 27.36223724160395 24.983513578839474 124-125 21.493977199369915 26.157721291678936 27.308972916851403 25.039328592099743 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1307.0 1 993.5 2 1092.5 3 1579.0 4 1198.5 5 859.0 6 1639.0 7 3767.5 8 6033.0 9 6897.0 10 5257.0 11 2924.5 12 2164.5 13 1981.5 14 1972.0 15 2150.5 16 2409.5 17 2678.5 18 2924.5 19 3129.0 20 3441.0 21 3973.0 22 4550.0 23 5354.5 24 6551.0 25 7924.5 26 9737.0 27 12185.0 28 14980.0 29 17713.5 30 20477.5 31 23744.0 32 27191.5 33 30495.5 34 33242.0 35 35389.5 36 38338.5 37 41870.5 38 44936.0 39 47338.0 40 50332.0 41 54300.5 42 57277.0 43 61001.5 44 64849.0 45 67791.0 46 69777.5 47 71709.0 48 78140.0 49 80127.5 50 77410.0 51 75020.0 52 71782.0 53 70404.0 54 68274.5 55 63489.5 56 57272.0 57 51385.5 58 46689.5 59 41940.5 60 36241.5 61 29983.5 62 23892.5 63 18658.0 64 14714.5 65 11838.0 66 9148.0 67 7180.0 68 5404.5 69 3811.0 70 2815.5 71 2232.0 72 1858.5 73 1532.5 74 1170.5 75 916.5 76 712.5 77 449.0 78 274.5 79 196.0 80 143.5 81 98.0 82 67.0 83 41.0 84 31.0 85 21.0 86 12.5 87 9.0 88 5.5 89 3.5 90 2.0 91 2.0 92 4.0 93 3.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10143823073526691 2 2.5850721390231117E-4 3 0.0 4 0.18322991321395815 5 0.0 6 1.0340288556092447E-4 7 0.038620977757005286 8 0.0 9 0.0 10-11 8.013723630971646E-4 12-13 9.04775248658089E-4 14-15 5.428651491948534E-4 16-17 0.0048599356213634495 18-19 0.0018354012187064093 20-21 0.004006861815485823 22-23 0.002533370696242649 24-25 0.0057647108700215385 26-27 0.005221845720826685 28-29 0.003981011094095592 30-31 0.0010081781342190135 32-33 5.170144278046224E-5 34-35 0.0015768940048040982 36-37 0.0014476403978529425 38-39 6.979694775362401E-4 40-41 0.0018612519400966405 42-43 0.0010857302983897069 44-45 0.001318386790901787 46-47 1.0340288556092447E-4 48-49 5.170144278046224E-5 50-51 0.04032712536876054 52-53 2.585072139023112E-5 54-55 0.1344237512292018 56-57 7.755216417069335E-5 58-59 2.585072139023112E-5 60-61 0.0 62-63 7.755216417069335E-4 64-65 0.06170567195848167 66-67 0.034717518827080386 68-69 0.001964654825657565 70-71 0.0032054894523886584 72-73 0.07212351267874481 74-75 0.004549726964680676 76-77 0.001551043283413867 78-79 0.0016027447261943292 80-81 2.5850721390231117E-4 82-83 1.551043283413867E-4 84-85 0.0 86-87 2.5850721390231117E-4 88-89 0.001370088233682249 90-91 3.102086566827734E-4 92-93 0.002249012760950107 94-95 0.004032712536876054 96-97 0.002274863482340338 98-99 0.0 100-101 0.0016027447261943292 102-103 0.0018354012187064093 104-105 2.3265649251208006E-4 106-107 7.755216417069335E-5 108-109 0.0 110-111 2.0680577112184895E-4 112-113 2.585072139023112E-5 114-115 7.755216417069335E-5 116-117 0.0 118-119 9.04775248658089E-4 120-121 0.002223162039559876 122-123 7.496709203167023E-4 124-125 0.00809127579514234 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1934182.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.97816167224886 #Duplication Level Percentage of deduplicated Percentage of total 1 74.83104236583544 38.14748975828463 2 11.506282614283101 11.731382707150205 3 5.0152342168522654 7.670022621926675 4 2.6590770529649346 5.422194396200539 5 1.6096112834165868 4.102751211774337 6 1.0231035599261868 3.1293563211222306 7 0.7040822105619562 2.5124971732407237 8 0.4909107956852531 2.0020583927276125 9 0.3701572469702318 1.698294238218272 >10 1.661810097222726 14.395711918502293 >50 0.08451074073002081 2.9294520130917903 >100 0.04106012658808965 3.5651219563293477 >500 0.0019745363433248562 0.6797609404337968 >1k 8.313837235052024E-4 0.6499343166738224 >5k 2.078459308763006E-4 0.8127887419636276 >10k+ 1.039229654381503E-4 0.5511832923601604 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10404 0.5379018106879291 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9359 0.483873802982346 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5983 0.3093297321555055 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2315 0.11968884003677006 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2203 0.1138982784453583 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.170144278046223E-5 0.0 0.0 0.0 0.0 7 5.170144278046223E-5 0.0 0.0 0.0 0.0 8 5.170144278046223E-5 0.0 0.0 0.0 0.0 9 5.170144278046223E-5 0.0 0.0 0.0 0.0 10-11 5.170144278046223E-5 0.0 0.0 0.0 0.0 12-13 5.170144278046223E-5 0.0 0.0 0.0 0.0 14-15 5.170144278046223E-5 0.0 0.0 0.0 0.0 16-17 1.551043283413867E-4 0.0 0.0 7.755216417069335E-5 0.0 18-19 3.877608208534667E-4 0.0 0.0 1.0340288556092446E-4 0.0 20-21 4.6531298502416006E-4 0.0 0.0 2.8435793529254226E-4 0.0 22-23 4.6531298502416006E-4 0.0 0.0 8.013723630971646E-4 0.0 24-25 5.687158705850845E-4 0.0 0.0 0.0012666853481213248 0.0 26-27 5.945665919753157E-4 0.0 0.0 0.001990505547047796 0.0 28-29 6.462680347557779E-4 0.0 0.0 0.0029211315170961163 0.0 30-31 6.72118756146009E-4 0.0 0.0 0.006669486118679628 0.0 32-33 6.72118756146009E-4 5.170144278046223E-5 0.0 0.012098137610628163 0.0 34-35 6.9796947753624E-4 5.170144278046223E-5 0.0 0.020008458356038886 0.0 36-37 7.238201989264712E-4 5.170144278046223E-5 0.0 0.03427805656344646 0.0 38-39 7.238201989264712E-4 5.170144278046223E-5 0.0 0.05684573633711823 0.0 40-41 7.238201989264712E-4 5.170144278046223E-5 0.0 0.08703937892090817 0.0 42-43 7.496709203167024E-4 5.170144278046223E-5 0.0 0.11596633615657678 0.0 44-45 8.272230844873957E-4 5.170144278046223E-5 0.0 0.14791782779490245 0.0 46-47 0.0010598795769994757 7.755216417069335E-5 0.0 0.1887619675914676 0.0 48-49 0.001137431741170169 1.0340288556092446E-4 0.0 0.23451774445217669 0.0 50-51 0.001137431741170169 1.0340288556092446E-4 0.0 0.28583142641178544 0.0 52-53 0.001137431741170169 1.0340288556092446E-4 0.0 0.3386185994906374 0.0 54-55 0.001137431741170169 1.0340288556092446E-4 0.0 0.39450785913631703 0.0 56-57 0.0012408346267310936 1.0340288556092446E-4 0.0 0.4591863640546753 0.0 58-59 0.0012925360695115557 1.0340288556092446E-4 0.0 0.5362732152403444 0.0 60-61 0.0012925360695115557 1.0340288556092446E-4 0.0 0.624682682394935 0.0 62-63 0.0012925360695115557 1.0340288556092446E-4 0.0 0.7112050468880384 0.0 64-65 0.001344237512292018 1.0340288556092446E-4 0.0 0.7992526039431657 0.0 66-67 0.0013959389550724803 1.0340288556092446E-4 0.0 0.9009493418923349 0.0 68-69 0.0013959389550724803 1.0340288556092446E-4 0.0 1.0221375237697383 0.0 70-71 0.0013959389550724803 1.0340288556092446E-4 0.0 1.1610334497994501 0.0 72-73 0.0013959389550724803 1.0340288556092446E-4 0.0 1.3086152182162796 0.0 74-75 0.0013959389550724803 1.0340288556092446E-4 0.0 1.454723495513866 0.0 76-77 0.0013959389550724803 1.0340288556092446E-4 0.0 1.613472775571275 0.0 78-79 0.0013959389550724803 1.0340288556092446E-4 0.0 1.796004719307697 0.0 80-81 0.0016544461689747916 1.0340288556092446E-4 0.0 2.0117289893091757 0.0 82-83 0.001757849054535716 1.0340288556092446E-4 0.0 2.242394976274208 0.0 84-85 0.0018612519400966403 1.0340288556092446E-4 0.0 2.4766542135124823 0.0 86-87 0.0019129533828771026 1.0340288556092446E-4 0.0 2.7177380411977774 0.0 88-89 0.0019129533828771026 1.0340288556092446E-4 0.0 2.9909801662925206 0.0 90-91 0.002016356268438027 1.0340288556092446E-4 0.0 3.297931632080125 0.0 92-93 0.0020680577112184894 1.0340288556092446E-4 0.0 3.6332930406755928 0.0 94-95 0.0020680577112184894 1.0340288556092446E-4 0.0 3.977236888772618 0.0 96-97 0.0020680577112184894 1.0340288556092446E-4 0.0 4.313554774059525 0.0 98-99 0.002249012760950107 1.0340288556092446E-4 0.0 4.683349343546781 0.0 100-101 0.002404117089291494 1.0340288556092446E-4 0.0 5.112548870788788 0.0 102-103 0.002610922860413343 1.0340288556092446E-4 0.0 5.575767947380339 0.0 104-105 0.0029469822384863473 1.0340288556092446E-4 0.0 6.038133950166014 0.0 106-107 0.0030503851240472717 1.0340288556092446E-4 0.0 6.494011421882739 0.0 108-109 0.003153788009608196 1.0340288556092446E-4 0.0 6.971784454616991 0.0 110-111 0.003179638730998427 1.0340288556092446E-4 0.0 7.522844282492548 0.0 112-113 0.0032054894523886584 1.0340288556092446E-4 0.0 8.098255489917701 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8710 0.0 38.295853 1 GTATCAA 12345 0.0 37.36341 1 TATCAAC 15345 0.0 29.873747 2 ATCAACG 15215 0.0 29.188688 3 TCAACGC 15275 0.0 28.722807 4 CAACGCA 15255 0.0 28.508934 5 AACGCAG 15395 0.0 27.863224 6 TCGACCG 75 5.3174826E-6 27.753988 18-19 GAGTACT 9245 0.0 27.499535 12-13 GTACATG 9955 0.0 26.913067 1 TACATGG 9975 0.0 25.57768 2 CGCAGAG 17485 0.0 24.9307 8 GTACTTT 10540 0.0 24.628922 14-15 AGTACTT 9850 0.0 24.602968 12-13 CAACGCG 195 1.4668785E-6 24.407944 6 ACGCAGA 17490 0.0 24.321592 7 GCAGAGT 18075 0.0 23.36018 9 ACATGGG 10350 0.0 22.92308 3 CATGGGG 4050 0.0 22.078344 4 GTACCGT 190 3.3095526E-5 21.918976 6 >>END_MODULE