FastQCFastQC Report
Thu 26 May 2016
SRR1364488_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364488_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2972494
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82450.045.4881941
GGTATCA52050.043.1190721
GTACATG134700.036.5941351
ATCAACG97050.036.052323
TCAACGC97750.035.580594
TATCAAC103900.035.5647772
TACATGG134500.035.215582
CAACGCA100750.034.8166735
AACGCAG99000.034.451636
ACATGGG136450.031.7038693
ACGCAGA115750.028.7976677
CGCAGAG116050.028.109628
CATGGGG60450.026.5016574
GCAGAGT123900.024.1185919
CATGGGA91450.023.3139744
GAGTACT47100.021.47005712-13
CGCATAG3108.411007E-921.1227558
CAGAGTA123800.020.9092410-11
AGAGTAC118650.020.58804310-11
AGTACAT99750.020.1030082
Produced by FastQC (version 0.11.5)