##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364488_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2972494 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.65394816608545 31.0 26.0 31.0 10.0 34.0 2 26.76279985762797 31.0 26.0 31.0 10.0 34.0 3 26.841954264667987 31.0 26.0 31.0 10.0 34.0 4 29.656600147889282 35.0 28.0 37.0 10.0 37.0 5 30.26630667715393 35.0 30.0 37.0 10.0 37.0 6 30.400357410309322 35.0 30.0 37.0 10.0 37.0 7 30.154925459900003 35.0 30.0 37.0 10.0 37.0 8 29.843201702005118 35.0 30.0 37.0 8.0 37.0 9 30.44751578977115 35.0 28.0 39.0 8.0 39.0 10-11 30.59089538952812 35.0 28.5 39.0 8.0 39.0 12-13 30.449132950310414 35.0 28.5 39.0 7.0 39.0 14-15 31.124605634191354 36.0 28.0 40.0 2.0 41.0 16-17 31.37694525203415 37.0 29.0 40.0 2.0 41.0 18-19 31.293795883187652 37.0 29.0 40.0 2.0 41.0 20-21 31.080345662598475 37.0 28.0 40.0 2.0 41.0 22-23 31.1442085333057 37.0 28.5 40.0 2.0 41.0 24-25 31.177878744246414 37.0 29.0 40.0 2.0 41.0 26-27 31.28539267026275 37.0 29.5 40.0 2.0 41.0 28-29 31.23559711138189 37.0 29.5 40.0 2.0 41.0 30-31 30.88015165043226 36.0 28.5 40.0 2.0 41.0 32-33 30.921756612460783 36.0 29.0 40.0 2.0 41.0 34-35 30.620284683501463 36.0 28.0 40.0 2.0 41.0 36-37 30.39243678877064 36.0 27.5 40.0 2.0 41.0 38-39 30.255367882996566 36.0 27.0 40.0 2.0 41.0 40-41 30.32330981996936 36.0 28.0 40.0 2.0 41.0 42-43 30.179042413542298 36.0 27.5 40.0 2.0 41.0 44-45 29.83025920321454 35.0 27.0 40.0 2.0 41.0 46-47 29.570796274105177 35.0 26.5 40.0 2.0 41.0 48-49 29.33261210956187 35.0 26.0 40.0 2.0 41.0 50-51 29.320184330060883 35.0 26.0 40.0 2.0 41.0 52-53 29.13553702715632 35.0 26.0 39.0 2.0 41.0 54-55 29.01818119733799 35.0 25.5 39.0 2.0 41.0 56-57 28.62477249743818 34.0 24.0 39.0 2.0 40.5 58-59 28.366021764888337 34.0 23.5 39.0 2.0 40.0 60-61 27.958465517508195 34.0 22.5 38.5 2.0 40.0 62-63 27.884612382733152 34.0 23.0 38.0 2.0 40.0 64-65 27.682614834546342 33.5 22.5 38.0 2.0 40.0 66-67 27.460008666123464 33.0 22.0 37.5 2.0 40.0 68-69 27.27102931074041 33.0 22.5 37.0 2.0 40.0 70-71 27.151692989119574 33.0 22.5 37.0 2.0 39.5 72-73 27.119276607454886 33.0 22.5 36.5 2.0 39.0 74-75 26.828737585340797 33.0 22.0 36.0 2.0 39.0 76-77 26.55569733698369 33.0 22.0 36.0 2.0 39.0 78-79 26.263119118154655 33.0 21.5 35.0 2.0 37.5 80-81 26.239713688236208 33.0 22.0 35.0 2.0 37.0 82-83 26.03772253198829 33.0 22.0 35.0 2.0 37.0 84-85 25.841422724486577 33.0 21.0 35.0 2.0 36.0 86-87 25.632076633291774 33.0 20.0 35.0 2.0 36.0 88-89 25.423719274117964 32.0 20.0 35.0 2.0 36.0 90-91 25.257271839741307 32.0 19.0 35.0 2.0 35.0 92-93 25.14973890611722 32.0 18.5 35.0 2.0 35.0 94-95 25.083118754823392 32.0 18.0 35.0 2.0 35.0 96-97 24.97447244636995 32.0 18.0 35.0 2.0 35.0 98-99 24.874151638321223 32.0 16.0 35.0 2.0 35.0 100-101 24.805575721935856 32.0 15.5 35.0 2.0 35.0 102-103 23.649473977071104 30.0 10.0 34.0 2.0 35.0 104-105 24.453263488504938 32.0 10.0 34.5 2.0 35.0 106-107 24.537317316704424 32.0 7.0 35.0 2.0 35.0 108-109 24.468885891779763 32.0 7.0 35.0 2.0 35.0 110-111 24.359187268334267 32.0 3.5 35.0 2.0 35.0 112-113 24.32434363198042 32.0 2.0 35.0 2.0 35.0 114-115 24.26515562352691 32.0 2.0 35.0 2.0 35.0 116-117 24.094564362451194 32.0 2.0 35.0 2.0 35.0 118-119 23.930755957791675 32.0 2.0 35.0 2.0 35.0 120-121 23.771093734756068 32.0 2.0 35.0 2.0 35.0 122-123 23.49313606688525 32.0 2.0 35.0 2.0 35.0 124-125 22.465698332780487 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.0669147404612325 1101 2 1.6826954286844291 1101 3 1.9184005739144325 1101 4 2.3560708343316676 1101 5 1.6715564120686466 1101 6 1.6325755954721863 1101 7 1.5743495565957168 1101 8 1.986831505637955 1101 9 2.4503720485856846 1101 10-11 2.4407883014913736 1101 12-13 2.2407459461397927 1101 14-15 2.6383078853822894 1101 16-17 2.2629559800222125 1101 18-19 2.212851321223141 1101 20-21 2.585490349950991 1101 22-23 2.5162833636206976 1101 24-25 2.385192698740667 1101 26-27 2.071903428343621 1101 28-29 1.9468971143239315 1101 30-31 2.1444014086730725 1101 32-33 2.32927077901239 1101 34-35 2.691229336831924 1101 36-37 2.742650145950801 1101 38-39 2.7259526421489717 1101 40-41 2.480797627286112 1101 42-43 2.4330508781502047 1101 44-45 2.5999402481128833 1101 46-47 2.6872805744164268 1101 48-49 2.604080904588912 1101 50-51 2.510255695519863 1101 52-53 2.372676962311779 1101 54-55 2.4055886182057975 1101 56-57 2.746421384362783 1101 58-59 2.779065442920075 1101 60-61 2.9271568764160705 1101 62-63 2.616712248633874 1101 64-65 2.535855695843395 1101 66-67 2.6423502673913433 1101 68-69 2.5476759817261367 1101 70-71 2.5382592563539816 1101 72-73 2.4349343211268 1101 74-75 2.3885798736714996 1101 76-77 2.6379380489715807 1101 78-79 2.6355125195431803 1101 80-81 2.5173645840154855 1101 82-83 2.4981037361546967 1101 84-85 2.3451335257296506 1101 86-87 2.3049534146031903 1101 88-89 2.5293913637442245 1101 90-91 2.529637966884664 1101 92-93 2.499872847056224 1101 94-95 2.384473924535019 1101 96-97 2.535428004323652 1101 98-99 2.4520740144601376 1101 100-101 2.35906746554053 1101 102-103 2.377728416391925 1101 104-105 2.3208027429470697 1101 106-107 2.3943524214835 1101 108-109 2.4171222514991406 1101 110-111 2.6484094947562795 1101 112-113 2.603324518835148 1101 114-115 2.5479562613660427 1101 116-117 2.6927523158342623 1101 118-119 2.645894376827936 1101 120-121 2.7031007305240706 1101 122-123 2.733944583805009 1101 124-125 2.6814839842406 1102 1 1.1231636230769695 1102 2 0.9068049379212901 1102 3 0.9512566840169789 1102 4 1.5535591852205677 1102 5 1.3579185730140964 1102 6 1.315450217134579 1102 7 1.18162901671759 1102 8 1.2613516040407582 1102 9 1.840477814905121 1102 10-11 1.562001894689594 1102 12-13 1.8926900080903017 1102 14-15 2.0979184974685054 1102 16-17 1.6665706127202355 1102 18-19 1.8257362855326988 1102 20-21 1.9061635465462707 1102 22-23 1.8551797499779603 1102 24-25 1.5855518515261764 1102 26-27 1.6298974858518527 1102 28-29 1.3564052018159458 1102 30-31 1.3335711981429874 1102 32-33 1.164512715829126 1102 34-35 1.5217698911585416 1102 36-37 1.7232223624695173 1102 38-39 1.8363943993620389 1102 40-41 1.3054416017083668 1102 42-43 1.3832217482790874 1102 44-45 1.83031512125228 1102 46-47 1.7441181732947122 1102 48-49 1.7590152986644085 1102 50-51 1.602698306118775 1102 52-53 1.7412339180566079 1102 54-55 1.5733244501508636 1102 56-57 1.6217562640594565 1102 58-59 1.7629857432930969 1102 60-61 2.167929117616872 1102 62-63 1.9367612830838326 1102 64-65 2.04317912983381 1102 66-67 1.9843596831601396 1102 68-69 1.713883928332308 1102 70-71 1.919426200253593 1102 72-73 1.8995098317215984 1102 74-75 1.9509539306746824 1102 76-77 1.8455637335700068 1102 78-79 1.797754260631521 1102 80-81 1.3660784521814655 1102 82-83 1.3180601918315311 1102 84-85 1.4025045536829026 1102 86-87 1.4885392820235026 1102 88-89 1.2562168246099148 1102 90-91 1.2425873294418501 1102 92-93 1.2569670783206064 1102 94-95 1.3794237671427148 1102 96-97 1.5285043537484064 1102 98-99 1.576421275382689 1102 100-101 1.5477495909746573 1102 102-103 1.3409293817951493 1102 104-105 1.3301650702574541 1102 106-107 1.6089586798085662 1102 108-109 1.7018944317917466 1102 110-111 1.416112259258277 1102 112-113 1.2796686665605002 1102 114-115 1.3322268929042878 1102 116-117 1.5721170148727097 1102 118-119 1.646532025187831 1102 120-121 1.5558492000799191 1102 122-123 1.6372586605857435 1102 124-125 1.5452001440143661 1103 1 1.4104024622144777 1103 2 1.155208484383838 1103 3 1.2973596131378642 1103 4 1.878808964015505 1103 5 1.3648751843015816 1103 6 1.3845753595797987 1103 7 1.3416136306012731 1103 8 1.6163339855486925 1103 9 2.286162740982654 1103 10-11 1.7419629263520662 1103 12-13 1.8607406545646406 1103 14-15 2.1746503163093465 1103 16-17 2.057626780513587 1103 18-19 1.6816842796465465 1103 20-21 2.191449861439299 1103 22-23 1.931604998249309 1103 24-25 2.002228850129164 1103 26-27 1.634792494747483 1103 28-29 1.5016601160913723 1103 30-31 1.8178520886000733 1103 32-33 1.7728118606157963 1103 34-35 1.7059803127286486 1103 36-37 2.0793191534039757 1103 38-39 2.1011103371698816 1103 40-41 2.0444615057602498 1103 42-43 2.079859541418138 1103 44-45 1.958994306838246 1103 46-47 2.1016883660988057 1103 48-49 2.16880745906213 1103 50-51 2.0566904477496344 1103 52-53 2.0516254831992953 1103 54-55 1.9763644360994306 1103 56-57 2.0912545249066348 1103 58-59 2.2715149261013003 1103 60-61 2.267300495510998 1103 62-63 2.049481438015448 1103 64-65 2.0358125862696212 1103 66-67 2.0711545962566973 1103 68-69 1.8962209665963812 1103 70-71 2.0072808566709774 1103 72-73 1.9051368837119007 1103 74-75 2.0028930403395506 1103 76-77 2.0363655917602834 1103 78-79 2.1420253459116623 1103 80-81 1.6983135091527757 1103 82-83 1.8627497967518352 1103 84-85 1.7781969204483516 1103 86-87 1.7705148464859803 1103 88-89 1.8714655515798597 1103 90-91 1.9498121391648446 1103 92-93 1.8897786573385744 1103 94-95 1.6801959184754303 1103 96-97 1.7892884816584562 1103 98-99 1.6883178394887963 1103 100-101 1.511110131175716 1103 102-103 1.631309891765973 1103 104-105 1.7866967086789778 1103 106-107 1.6230853584941514 1103 108-109 1.9163654434271997 1103 110-111 1.9620302636566365 1103 112-113 1.7926347808581369 1103 114-115 1.751861704686899 1103 116-117 1.8973762787239394 1103 118-119 2.0529578374812765 1103 120-121 1.942767215657092 1103 122-123 1.974372678076186 1103 124-125 1.9706526828251825 1104 1 1.3647714938679236 1104 2 1.23698089143517 1104 3 1.296964066271702 1104 4 1.5367869972685888 1104 5 1.1764416985448243 1104 6 1.048235339543929 1104 7 1.0675281711496751 1104 8 1.4746938314017513 1104 9 1.9441769680666283 1104 10-11 1.7580873790011609 1104 12-13 1.3365611759820162 1104 14-15 1.527651697814946 1104 16-17 1.292264357465875 1104 18-19 0.7179483572693854 1104 20-21 -3.6459916669421553 1104 22-23 -0.4847762245851186 1104 24-25 0.9065670200785902 1104 26-27 1.1433026702229334 1104 28-29 0.7855094673492609 1104 30-31 1.410941975332772 1104 32-33 1.3332348558168832 1104 34-35 1.5007032643032403 1104 36-37 1.5708559370004131 1104 38-39 1.557549627673847 1104 40-41 1.6501421800319243 1104 42-43 1.5651195431955145 1104 44-45 1.6910889440112449 1104 46-47 1.6972815248184503 1104 48-49 1.7539122232367035 1104 50-51 1.6138303676061518 1104 52-53 1.6583756528578775 1104 54-55 1.5824694588013664 1104 56-57 1.964529824232848 1104 58-59 1.8178404847627725 1104 60-61 1.9045999348297755 1104 62-63 1.523007791648567 1104 64-65 1.6617605535982278 1104 66-67 1.6210781893023132 1104 68-69 1.6376311727719504 1104 70-71 1.8125044020506103 1104 72-73 1.6637034504685104 1104 74-75 1.6454449275893026 1104 76-77 1.5863978874832725 1104 78-79 1.6339240476195585 1104 80-81 1.3586680753923375 1104 82-83 1.3952596531075656 1104 84-85 1.3451285036052916 1104 86-87 1.2529804300307141 1104 88-89 1.406065096733279 1104 90-91 1.3634870450447245 1104 92-93 1.1410071268565858 1104 94-95 1.2707696876428116 1104 96-97 1.3676960136916136 1104 98-99 1.2120092870804555 1104 100-101 0.7537497561494568 1104 102-103 1.1491126919404806 1104 104-105 1.374038461656081 1104 106-107 1.474931506436434 1104 108-109 1.5525814107234908 1104 110-111 1.6199403122920941 1104 112-113 1.5554313400205082 1104 114-115 1.4699188715501528 1104 116-117 1.4520018583907657 1104 118-119 1.480648117660305 1104 120-121 1.5144269815282456 1104 122-123 1.3816609078100655 1104 124-125 1.552609250228116 1105 1 1.3374337042266866 1105 2 1.1081723563012886 1105 3 1.1832647912582317 1105 4 1.7110246347015732 1105 5 1.2984338033820961 1105 6 1.2456817644937885 1105 7 1.2290450912577562 1105 8 1.5441400023297724 1105 9 1.9372949427750683 1105 10-11 1.6621301980700096 1105 12-13 1.7901121555025306 1105 14-15 2.038333787431391 1105 16-17 1.8272020299292961 1105 18-19 1.4887501239503251 1105 20-21 1.7392459766365214 1105 22-23 1.6218290398126918 1105 24-25 1.6280477830454885 1105 26-27 1.5924297979119295 1105 28-29 1.1562741755211903 1105 30-31 1.610259967658859 1105 32-33 1.4669227613043674 1105 34-35 1.3236460049121135 1105 36-37 1.5943384945087047 1105 38-39 1.5419955630170925 1105 40-41 1.6885289364802922 1105 42-43 1.7939434425537009 1105 44-45 1.984056963890506 1105 46-47 2.2690991944958228 1105 48-49 1.960131169578684 1105 50-51 2.0224419948413903 1105 52-53 1.9525114562121964 1105 54-55 1.924198548604231 1105 56-57 2.267696226976284 1105 58-59 2.0583832106219937 1105 60-61 2.426193635088101 1105 62-63 2.170466487492238 1105 64-65 1.8736396596871714 1105 66-67 2.0486089945720387 1105 68-69 1.5879462622839213 1105 70-71 1.7072604079258902 1105 72-73 1.635937306253755 1105 74-75 2.064388094299332 1105 76-77 1.8730445533441689 1105 78-79 1.7482672680019427 1105 80-81 1.730412508933874 1105 82-83 1.827090904759224 1105 84-85 1.7613652519618341 1105 86-87 1.888154363292621 1105 88-89 1.879372522570172 1105 90-91 1.959654367593057 1105 92-93 1.8880082761005603 1105 94-95 1.7596527290217416 1105 96-97 1.850320489515493 1105 98-99 1.7167619128263851 1105 100-101 1.7811365064521318 1105 102-103 1.9091992437373797 1105 104-105 1.9621482933256367 1105 106-107 2.0486705645480257 1105 108-109 2.1357316503029615 1105 110-111 1.9004066267424555 1105 112-113 1.9862551902131749 1105 114-115 1.9776889285206956 1105 116-117 2.1922923241725876 1105 118-119 2.2737364906299256 1105 120-121 2.316903673999981 1105 122-123 2.354443204945966 1105 124-125 1.9455557806789834 1106 1 -0.25399851922760774 1106 2 0.06185557361511229 1106 3 0.05050786596179435 1106 4 -0.2514906610932073 1106 5 -0.7120860782560179 1106 6 -0.11694970465607568 1106 7 -0.09619389740636208 1106 8 0.29853808353934497 1106 9 0.4731228088390367 1106 10-11 -2.333096783888209 1106 12-13 -0.17683919350642796 1106 14-15 0.1780387504101597 1106 16-17 -0.04159315570723621 1106 18-19 -2.883089954917317 1106 20-21 -5.378342076875725 1106 22-23 -6.197892092107164 1106 24-25 -6.064316504170897 1106 26-27 -4.853598095441537 1106 28-29 -1.3667504117084377 1106 30-31 -3.4491470866920473 1106 32-33 -4.074693422875882 1106 34-35 -3.296673305660619 1106 36-37 -5.854054907878574 1106 38-39 -6.224191126322566 1106 40-41 -2.2916151037107895 1106 42-43 -0.950732462820568 1106 44-45 -0.1779639573833336 1106 46-47 0.029634810389985944 1106 48-49 0.2960721376709543 1106 50-51 0.12297080011037664 1106 52-53 0.050325997417232315 1106 54-55 0.08627665865406797 1106 56-57 0.19021798094891196 1106 58-59 0.43391081169591317 1106 60-61 0.5786705762211746 1106 62-63 0.3520022079457803 1106 64-65 0.18736759249795654 1106 66-67 -0.14433628529380726 1106 68-69 0.009510702160348217 1106 70-71 0.2494550672746314 1106 72-73 0.2500325227546014 1106 74-75 0.22707694523206712 1106 76-77 0.1515280463053763 1106 78-79 0.16479867601058373 1106 80-81 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-8.248394106946222 2216 104-105 -10.83725692529133 2216 106-107 -7.716443620147054 2216 108-109 -10.24146856029765 2216 110-111 -7.705480709164998 2216 112-113 -7.051299016451331 2216 114-115 -7.400160677016512 2216 116-117 -6.89187876143842 2216 118-119 -7.160938904904992 2216 120-121 -8.038332251901256 2216 122-123 -10.778359855066423 2216 124-125 -9.281394354614857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 252036.0 3 39541.0 4 23028.0 5 19038.0 6 18906.0 7 21222.0 8 23422.0 9 25150.0 10 26559.0 11 27286.0 12 27802.0 13 28315.0 14 28850.0 15 24837.0 16 20574.0 17 18435.0 18 16810.0 19 16147.0 20 16346.0 21 17667.0 22 22270.0 23 30340.0 24 41067.0 25 52241.0 26 62560.0 27 71312.0 28 82263.0 29 93417.0 30 105025.0 31 118722.0 32 141197.0 33 171850.0 34 212024.0 35 267302.0 36 348507.0 37 361374.0 38 98778.0 39 274.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95564134304414 18.855796649528624 14.02028278441763 23.168279223009606 2 14.967698773606717 20.450611951504243 40.3064182807745 24.275270994114535 3 18.569827513672696 25.713790492217083 29.262229701304165 26.45415229280606 4 11.330944271721595 16.658504544366775 38.02690706725353 33.9836441166581 5 13.704371663054973 38.55386202054188 33.4937557768573 14.248010539545849 6 31.4411654178973 36.78796848196049 17.76269154179814 14.00817455834407 7 28.50038503941061 32.789444840118655 20.898741531740267 17.811428588730475 8 25.659429904831626 35.85372976057187 19.999791415468092 18.487048919128412 9 26.11891137530179 16.897455946039784 20.343986940594778 36.63964573806364 10-11 24.325702006507 27.40732962125079 27.34884976432197 20.918118607920242 12-13 25.206900536847108 24.75192711004735 27.81925479975433 22.22191755335121 14-15 22.52773309875374 25.90090828937069 25.62877980758215 25.942578804293426 16-17 22.391613780293294 29.40868772250974 25.787844322258042 22.411854174938924 18-19 22.36647206285647 28.056665040294764 27.74150781499739 21.83535508185137 20-21 23.229784643743706 27.126082566987016 27.75476221676571 21.889370572503562 22-23 22.94000234863976 27.194590187598443 27.42852883964746 22.43687862411434 24-25 22.613497337114445 27.262813412275516 27.650721879538242 22.47296737107179 26-27 22.617148092266703 27.52924864701824 27.208647778613475 22.64495548210158 28-29 22.524409293581368 27.432930585456326 27.508443709667596 22.534216411294704 30-31 22.48360131105405 27.185413892900307 27.688085539800518 22.642899256245126 32-33 22.533677133529974 27.256298134522815 27.37674483702518 22.83327989492203 34-35 22.79656654093468 27.13847567069211 27.36543256402405 22.69952522434916 36-37 22.839617277678904 26.959281924646493 27.368632649147656 22.832468148526942 38-39 22.725063602380278 27.032613978342134 27.46842255162168 22.77389986765591 40-41 22.736490693158608 27.02993804413304 27.518265370338586 22.71530589236977 42-43 22.76736369659262 27.04183828282118 27.259890760099882 22.93090726048632 44-45 22.713145502273065 27.19829296337436 27.24972230848042 22.83883922587216 46-47 22.7759823385299 27.350146988271216 26.989859188667808 22.884011484531076 48-49 22.88485478574505 27.223248411929372 26.782323293496983 23.109573508828596 50-51 22.819635473295737 27.444916482467534 26.783751087824925 22.951696956411805 52-53 23.054429000447332 27.285561938511094 26.76072474201014 22.899284319031434 54-55 22.813845888043648 27.244403553476687 26.778374375427198 23.163376183052467 56-57 22.70873930784262 27.504363665236557 26.799633411746974 22.98726361517385 58-59 22.93290605717454 27.0723345136254 26.753542937098512 23.241216492101543 60-61 22.818484107217433 26.969001897650703 27.19339513906626 23.019118856065603 62-63 22.803608486058888 27.225814005442405 26.974671423611206 22.995906084887505 64-65 22.65387803880192 27.204796812084147 26.85167739712754 23.289647751986394 66-67 22.695864066220697 27.42265994769837 26.655967458256686 23.22550852782425 68-69 22.559911688549388 27.261046333231054 26.875743554959815 23.303298423259744 70-71 22.47245483406959 27.167904942409958 27.13451380399755 23.225126419522898 72-73 22.70023673922251 27.115909366926953 26.847664349064697 23.33618954478583 74-75 22.59770721276576 27.347809148810477 26.777354184356888 23.277129454066877 76-77 22.73274233621228 27.14244306884931 26.902806448470894 23.22200814646752 78-79 22.711738653140024 27.16117987995945 26.916623554697523 23.210457912203 80-81 22.645789733887568 27.51693386913446 26.743258707735556 23.09401768924241 82-83 22.655282086461288 27.36037064593721 26.762572525308055 23.221774742293448 84-85 22.748757606051566 27.178346422983683 26.689429520689856 23.383466450274888 86-87 22.628393272398423 27.124647417264175 26.847224016536398 23.399735293801 88-89 22.72362461811653 26.97060786276638 26.856183707123954 23.44958381199314 90-91 22.61255737316084 27.27743587285006 26.92900405155237 23.181002702436736 92-93 22.614934369356394 27.09592139933091 26.809098530745608 23.480045700567086 94-95 22.49166862779764 27.03092284403298 26.837069463062807 23.640339065106577 96-97 22.611284220272008 27.004434749434303 26.863741970385213 23.52053905990848 98-99 22.512358368895484 26.987360458972503 26.753641906641434 23.746639265490582 100-101 22.41508081893833 27.03758093248351 26.839692704679912 23.707645543898252 102-103 22.452574826608966 26.672143589577967 27.12957430717598 23.745707276637088 104-105 22.49275415952456 26.73226472255538 27.14476037000466 23.630220747915402 106-107 22.295776813297756 26.900262441584793 27.035957625827134 23.768003119290317 108-109 22.4143249988057 26.77013672877696 26.832189833719884 23.98334843869745 110-111 22.191504722738447 26.950846244215164 27.075205858644853 23.782443174401532 112-113 22.226203332694112 26.92193434868472 27.03186346248953 23.819998856131637 114-115 22.16098148900434 26.903476877866456 26.99538844158258 23.940153191546624 116-117 21.92911332783824 26.980621393846622 27.151395360573282 23.938869917741854 118-119 22.01199705961018 27.022384306258086 26.805673539351265 24.159945094780472 120-121 21.953021925938536 27.090589565022256 26.950630681308745 24.005757827730463 122-123 21.960204726004193 26.98851048812006 26.989788932240106 24.06149585363564 124-125 22.0674526657777 27.02596900535988 26.633087260509846 24.27349106835257 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 868.0 1 957.0 2 1234.0 3 1317.0 4 1083.5 5 881.0 6 814.5 7 915.5 8 1151.5 9 1310.0 10 1310.0 11 1283.0 12 1338.0 13 1480.5 14 1687.0 15 1882.5 16 2098.0 17 2324.0 18 2541.5 19 2933.5 20 3438.0 21 4133.0 22 5264.5 23 6644.5 24 8410.5 25 11066.0 26 14625.0 27 18781.0 28 23251.5 29 28160.5 30 34263.0 31 40731.0 32 46774.0 33 52192.0 34 57206.5 35 61767.5 36 66817.5 37 72685.5 38 77428.0 39 80642.5 40 84186.5 41 89344.0 42 93436.0 43 97948.5 44 104168.0 45 108334.0 46 109404.0 47 109738.5 48 112047.0 49 113314.0 50 111128.0 51 108478.5 52 106710.0 53 105092.0 54 101826.5 55 94926.0 56 86498.0 57 79110.5 58 73866.5 59 68006.5 60 58766.5 61 48332.0 62 38633.0 63 29975.0 64 22858.0 65 17850.0 66 13816.5 67 10610.5 68 8019.0 69 5741.5 70 4124.5 71 3097.0 72 2410.5 73 1977.0 74 1559.5 75 1212.5 76 856.0 77 531.5 78 361.5 79 233.0 80 170.0 81 126.5 82 90.0 83 66.0 84 52.0 85 42.5 86 35.0 87 28.5 88 17.0 89 12.0 90 12.5 91 11.5 92 8.0 93 5.5 94 4.5 95 4.5 96 4.0 97 2.5 98 3.0 99 4.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0969556204318663 2 0.03145506769736121 3 0.0418503788401255 4 0.04888151161953565 5 0.053423152410063734 6 0.0033978201469876813 7 0.0023885666379814393 8 0.002624059123416229 9 0.007737610235714521 10-11 0.046610691224271604 12-13 0.007821714694798374 14-15 0.012396997268960003 16-17 0.02402023351434856 18-19 0.02166530866000066 20-21 0.13359152280879288 22-23 0.018873040618416725 24-25 0.017830145325776942 26-27 0.008830968203804617 28-29 0.005483610732267248 30-31 0.002573596447965917 32-33 0.007569401317546814 34-35 0.018973965969317347 36-37 0.0034987454978883054 38-39 0.010681266303649394 40-41 0.03444918644074639 42-43 0.01692181716767132 44-45 0.11344009441230159 46-47 0.004978983977764127 48-49 0.011236355733602826 50-51 0.01323804185979854 52-53 0.014449146070606031 54-55 0.012195146567158756 56-57 0.007468475966646191 58-59 0.016467653088618514 60-61 0.031118649861025794 62-63 0.009352415850124508 64-65 0.009083281581056176 66-67 0.010479415601848145 68-69 0.00962155011919284 70-71 0.004995804869580897 72-73 8.410445908385349E-4 74-75 0.0014129549126087387 76-77 0.009604729227376069 78-79 0.019562697182904324 80-81 0.004121118495108821 82-83 0.005702282325885267 84-85 0.0036164917406057 86-87 0.004777133275962879 88-89 0.007384371507562337 90-91 0.005937774811320056 92-93 0.0034314619306212227 94-95 0.004861237735046732 96-97 0.002018507018012484 98-99 0.003885626009674031 100-101 0.003666954416056012 102-103 7.401192399379107E-4 104-105 0.003330536579720598 106-107 7.737610235714522E-4 108-109 0.001345671345341656 110-111 0.002220357719813732 112-113 0.004171581170559133 114-115 0.00240538752979821 116-117 0.004844416843229961 118-119 0.003330536579720598 120-121 0.006476043349456719 122-123 0.004407073655993923 124-125 0.05468471929632154 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2972494.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.43615624548745 #Duplication Level Percentage of deduplicated Percentage of total 1 77.48180570039209 43.727752926898276 2 11.151931767125648 12.587443272970358 3 4.4191663971765065 7.482022957975835 4 2.2390572703841416 5.054551438159763 5 1.2797431360559246 3.6111891790271136 6 0.7914021718492549 2.6798217974101597 7 0.533238152907032 2.106573819946147 8 0.3822963267425527 1.72602681824949 9 0.28084410292165896 1.4264785505799453 >10 1.3304812161069288 13.043980390394319 >50 0.07594529907085211 2.901041326388299 >100 0.03315325213792907 3.1439009477337243 >500 6.858185615663515E-4 0.2573880072482365 >1k 2.4938856781961426E-4 0.2518285670182343 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.72835672670828E-5 0.0 0.0 0.0 0.0 7 6.72835672670828E-5 0.0 0.0 0.0 0.0 8 6.72835672670828E-5 0.0 0.0 0.0 0.0 9 6.72835672670828E-5 0.0 0.0 0.0 0.0 10-11 8.41044590838535E-5 0.0 0.0 0.0 0.0 12-13 1.0092535090062419E-4 0.0 0.0 0.0 0.0 14-15 1.0092535090062419E-4 0.0 0.0 0.0 0.0 16-17 1.6820891816770697E-4 0.0 0.0 1.1774624271739488E-4 0.0 18-19 1.6820891816770697E-4 0.0 0.0 2.0185070180124835E-4 0.0 20-21 1.6820891816770697E-4 0.0 0.0 4.037014036024968E-4 0.0 22-23 1.6820891816770697E-4 0.0 0.0 7.2329834812114E-4 0.0 24-25 1.6820891816770697E-4 0.0 0.0 0.0011438206435404074 0.0 26-27 1.6820891816770697E-4 0.0 0.0 0.0014970593716925923 0.0 28-29 1.6820891816770697E-4 0.0 0.0 0.0022035368279969617 0.0 30-31 1.6820891816770697E-4 0.0 0.0 0.00433979008872684 0.0 32-33 1.6820891816770697E-4 0.0 0.0 0.008830968203804617 0.0 34-35 1.6820891816770697E-4 0.0 0.0 0.015290190661444564 0.0 36-37 1.6820891816770697E-4 0.0 0.0 0.027401232769519467 0.0 38-39 1.6820891816770697E-4 0.0 0.0 0.048023646136880344 0.0 40-41 1.6820891816770697E-4 0.0 0.0 0.0745670134237445 0.0 42-43 1.6820891816770697E-4 0.0 0.0 0.09983199293253409 0.0 44-45 1.6820891816770697E-4 0.0 0.0 0.12834340456196042 0.0 46-47 1.6820891816770697E-4 0.0 0.0 0.1653325456670392 0.0 48-49 1.6820891816770697E-4 0.0 0.0 0.2084444913934225 0.0 50-51 2.0185070180124838E-4 0.0 0.0 0.2594286144900545 0.0 52-53 2.0185070180124838E-4 0.0 0.0 0.3091679915922454 0.0 54-55 2.0185070180124838E-4 0.0 0.0 0.3613127562242346 0.0 56-57 2.0185070180124838E-4 0.0 0.0 0.4241724289435067 0.0 58-59 2.0185070180124838E-4 0.0 0.0 0.4982179947209313 0.0 60-61 2.523133772515605E-4 0.0 0.0 0.5807917526494587 0.0 62-63 2.691342690683312E-4 0.0 0.0 0.6681426438539488 0.0 64-65 2.691342690683312E-4 0.0 0.0 0.7546356695757839 0.0 66-67 2.8595516088510185E-4 0.0 0.0 0.8607923178314236 0.0 68-69 3.027760527018726E-4 0.0 0.0 0.9835511863102163 0.0 70-71 3.027760527018726E-4 0.0 0.0 1.1209105888859658 3.36417836335414E-5 72-73 3.7005961996895535E-4 0.0 0.0 1.2683120638763274 3.36417836335414E-5 74-75 3.7005961996895535E-4 0.0 0.0 1.4174629116156332 3.36417836335414E-5 76-77 3.7005961996895535E-4 0.0 0.0 1.580995621858278 3.36417836335414E-5 78-79 3.7005961996895535E-4 0.0 0.0 1.7741162808066222 3.36417836335414E-5 80-81 3.7005961996895535E-4 0.0 0.0 1.9944867844981353 3.36417836335414E-5 82-83 3.7005961996895535E-4 0.0 0.0 2.224983465063344 3.36417836335414E-5 84-85 3.7005961996895535E-4 0.0 0.0 2.4555474291958204 3.36417836335414E-5 86-87 3.7005961996895535E-4 0.0 0.0 2.6988448084335914 3.36417836335414E-5 88-89 3.7005961996895535E-4 0.0 0.0 2.975228881874951 3.36417836335414E-5 90-91 3.7005961996895535E-4 0.0 0.0 3.290031872225814 3.36417836335414E-5 92-93 3.8688051178572603E-4 0.0 0.0 3.627728096339303 3.36417836335414E-5 94-95 4.0370140360249676E-4 0.0 0.0 3.9778213177217516 6.72835672670828E-5 96-97 4.0370140360249676E-4 0.0 0.0 4.3255259724662185 6.72835672670828E-5 98-99 4.0370140360249676E-4 0.0 0.0 4.692574652799972 6.72835672670828E-5 100-101 4.0370140360249676E-4 0.0 0.0 5.113752963000094 6.72835672670828E-5 102-103 4.205222954192675E-4 0.0 0.0 5.571079369714456 6.72835672670828E-5 104-105 4.373431872360382E-4 0.0 0.0 6.033788461810184 6.72835672670828E-5 106-107 4.373431872360382E-4 0.0 0.0 6.4918718086563 6.72835672670828E-5 108-109 4.373431872360382E-4 3.36417836335414E-5 0.0 6.976330313871113 6.72835672670828E-5 110-111 4.7098497086957953E-4 3.36417836335414E-5 0.0 7.510191778351781 6.72835672670828E-5 112-113 4.7098497086957953E-4 3.36417836335414E-5 0.0 8.09041162067947 6.72835672670828E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8245 0.0 45.488194 1 GGTATCA 5205 0.0 43.119072 1 GTACATG 13470 0.0 36.594135 1 ATCAACG 9705 0.0 36.05232 3 TCAACGC 9775 0.0 35.58059 4 TATCAAC 10390 0.0 35.564777 2 TACATGG 13450 0.0 35.21558 2 CAACGCA 10075 0.0 34.816673 5 AACGCAG 9900 0.0 34.45163 6 ACATGGG 13645 0.0 31.703869 3 ACGCAGA 11575 0.0 28.797667 7 CGCAGAG 11605 0.0 28.10962 8 CATGGGG 6045 0.0 26.501657 4 GCAGAGT 12390 0.0 24.118591 9 CATGGGA 9145 0.0 23.313974 4 GAGTACT 4710 0.0 21.470057 12-13 CGCATAG 310 8.411007E-9 21.122755 8 CAGAGTA 12380 0.0 20.90924 10-11 AGAGTAC 11865 0.0 20.588043 10-11 AGTACAT 9975 0.0 20.103008 2 >>END_MODULE