FastQCFastQC Report
Thu 26 May 2016
SRR1364488_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364488_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2972494
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81960.27572805866050526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74440.25042943736808215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43690.14698095269494235No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71300.059.2260361
GTATCAA116500.047.3570631
TATCAAC155200.035.4739762
ATCAACG150750.034.623933
TCAACGC152250.033.8914534
CAACGCA154300.033.2720645
AACGCAG156450.032.5486566
GTACATG133000.029.3736971
TACATGG132150.028.315362
ACGCAGA183150.027.4788327
CGCAGAG185800.027.1440988
ACATGGG133550.026.144683
GAGTACT113800.025.47754712-13
GCAGAGT195900.024.6516979
AGTACTT118350.023.76939612-13
GTACTTT128650.023.09163314-15
CAGAGTA190800.023.01957910-11
AGAGTAC186400.022.70148710-11
CATGGGA91250.021.3522914
GTGGTAT41900.020.6406291
Produced by FastQC (version 0.11.5)