FastQCFastQC Report
Thu 26 May 2016
SRR1364487_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364487_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3180074
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64250.052.2544361
GGTATCA38400.047.900981
CAACGCA78800.040.6770445
ATCAACG76700.040.4936833
TCAACGC77850.039.79654
AACGCAG80000.039.375536
TATCAAC86650.038.315792
GTACATG93450.033.060181
ACGCAGA93800.032.8207477
CGCAGAG93150.032.5409248
TACATGG95150.030.265562
ACATGGG98000.026.8961323
GCAGAGT104050.026.556929
AGAGTAC94000.022.98446710-11
CAGAGTA100400.022.61603510-11
GAGTACT56000.021.8819212-13
GTATAGG12550.020.8701761
CATGGGG57400.020.1367784
CATGGGA55000.019.6072224
GAGTACA76250.019.517191
Produced by FastQC (version 0.11.5)