##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364487_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3180074 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.992188232097742 28.0 16.0 31.0 2.0 31.0 2 23.313567860370544 28.0 16.0 31.0 2.0 34.0 3 23.281689356914335 28.0 16.0 31.0 2.0 34.0 4 25.775798613491386 32.0 17.0 35.0 2.0 37.0 5 26.211728406320105 32.0 19.0 35.0 2.0 37.0 6 26.280430266717065 32.0 19.0 35.0 2.0 37.0 7 25.998084006850156 32.0 17.0 35.0 2.0 37.0 8 25.64422211558599 32.0 17.0 35.0 2.0 37.0 9 26.111381055912535 33.0 13.0 37.0 2.0 39.0 10-11 26.095375767985274 32.5 13.0 37.0 2.0 39.0 12-13 25.914420544930714 32.5 10.5 37.0 2.0 39.0 14-15 26.352190074822158 32.5 11.0 38.0 2.0 40.0 16-17 26.52568760978518 33.0 11.0 38.0 2.0 40.0 18-19 26.453775289505842 33.0 10.0 38.5 2.0 40.0 20-21 26.220991398313373 32.5 10.0 39.0 2.0 40.0 22-23 26.22606832419623 33.0 10.0 39.0 2.0 40.0 24-25 26.21066836809458 33.0 10.0 39.0 2.0 40.0 26-27 26.240204001542104 33.0 10.0 39.0 2.0 40.0 28-29 26.127175342460585 33.0 9.0 39.0 2.0 40.0 30-31 25.77941865503759 32.0 8.0 38.5 2.0 40.0 32-33 25.728277549516143 32.5 8.0 38.0 2.0 40.0 34-35 25.43954417412928 32.0 4.5 38.0 2.0 40.0 36-37 25.209000010691575 32.0 2.0 38.0 2.0 40.0 38-39 25.04435399930945 32.0 2.0 38.0 2.0 40.0 40-41 25.083066148775153 32.0 2.0 38.0 2.0 40.0 42-43 24.92433666637946 32.0 2.0 38.0 2.0 40.0 44-45 24.60139355247708 32.0 2.0 38.0 2.0 40.0 46-47 24.344969488131408 31.0 2.0 38.0 2.0 40.0 48-49 24.09439041355641 31.0 2.0 38.0 2.0 40.0 50-51 24.06705126987611 31.0 2.0 38.0 2.0 40.0 52-53 23.864240423336064 31.0 2.0 38.0 2.0 40.0 54-55 23.732505595781735 31.0 2.0 38.0 2.0 40.0 56-57 23.35490683550131 30.0 2.0 37.0 2.0 40.0 58-59 23.135380969122103 30.0 2.0 37.0 2.0 40.0 60-61 22.759167711191626 29.5 2.0 37.0 2.0 40.0 62-63 22.680205869423162 29.5 2.0 36.0 2.0 40.0 64-65 22.509188937112782 29.5 2.0 36.0 2.0 39.5 66-67 22.296050029024485 29.0 2.0 36.0 2.0 39.0 68-69 22.136364279573367 29.0 2.0 35.5 2.0 39.0 70-71 22.046520143870865 29.0 2.0 35.0 2.0 39.0 72-73 22.00502205294594 29.0 2.0 35.0 2.0 39.0 74-75 21.754029465980977 29.0 2.0 35.0 2.0 38.0 76-77 21.531997997530876 29.0 2.0 35.0 2.0 37.0 78-79 21.255247991084484 29.0 2.0 35.0 2.0 37.0 80-81 21.245810160392494 29.0 2.0 34.5 2.0 36.0 82-83 21.071570818792267 29.0 2.0 34.0 2.0 36.0 84-85 20.895590322740915 29.0 2.0 34.0 2.0 36.0 86-87 20.715070152455574 29.0 2.0 34.0 2.0 35.0 88-89 20.520881275089828 29.0 2.0 34.0 2.0 35.0 90-91 20.365218702457867 29.0 2.0 34.0 2.0 35.0 92-93 20.25532015921642 29.0 2.0 34.0 2.0 35.0 94-95 20.17576870852691 29.0 2.0 34.0 2.0 35.0 96-97 20.06113678486727 28.0 2.0 34.0 2.0 35.0 98-99 19.957741549410486 28.0 2.0 34.0 2.0 35.0 100-101 19.89543639550526 28.0 2.0 34.0 2.0 35.0 102-103 18.9891826416618 25.5 2.0 32.5 2.0 34.5 104-105 19.59869786049004 27.0 2.0 34.0 2.0 35.0 106-107 19.67817305509243 27.5 2.0 34.0 2.0 35.0 108-109 19.606200358859574 27.5 2.0 34.0 2.0 35.0 110-111 19.493494491008697 27.0 2.0 34.0 2.0 35.0 112-113 19.451560403940285 27.0 2.0 34.0 2.0 35.0 114-115 19.39023635927969 27.0 2.0 34.0 2.0 35.0 116-117 19.22360737517429 27.0 2.0 34.0 2.0 35.0 118-119 19.061918999369198 27.0 2.0 34.0 2.0 35.0 120-121 18.89635005348932 27.0 2.0 34.0 2.0 35.0 122-123 18.62382054631433 26.5 2.0 34.0 2.0 35.0 124-125 17.77437537617049 22.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3020942814221819 1101 2 0.9871842410063678 1101 3 1.4266987661021915 1101 4 1.7357213368755495 1101 5 1.3194356864777994 1101 6 1.2429071217807852 1101 7 1.1609881345672193 1101 8 1.5618018951323407 1101 9 1.8317516488915793 1101 10-11 1.902358933818011 1101 12-13 1.5618302846012284 1101 14-15 1.8792787032941938 1101 16-17 1.6724652662206303 1101 18-19 1.5699037967051659 1101 20-21 1.9359653810865751 1101 22-23 1.8306328538921797 1101 24-25 1.6917034314044166 1101 26-27 1.2952842554962665 1101 28-29 1.3197238619966214 1101 30-31 1.4570047857188726 1101 32-33 1.7102032116704322 1101 34-35 1.9684803348057613 1101 36-37 1.8827253255800294 1101 38-39 1.9418898630381989 1101 40-41 1.7501170094591387 1101 42-43 1.7786641004465267 1101 44-45 2.0296755631829484 1101 46-47 1.9932001844361515 1101 48-49 1.947136544645499 1101 50-51 1.9170396497510325 1101 52-53 1.8041578832724454 1101 54-55 1.8472838551141209 1101 56-57 2.0674535939680148 1101 58-59 2.03272562629909 1101 60-61 2.1884660300107512 1101 62-63 1.945399456243468 1101 64-65 1.8108148434821665 1101 66-67 1.9281766697156613 1101 68-69 1.816313708159008 1101 70-71 1.774213506299084 1101 72-73 1.731832028829249 1101 74-75 1.7089570959688025 1101 76-77 1.9403001773552333 1101 78-79 1.823350474445757 1101 80-81 1.6600296172716469 1101 82-83 1.6488851935887539 1101 84-85 1.591904538026423 1101 86-87 1.4989878304395887 1101 88-89 1.6534761691766313 1101 90-91 1.648170744833216 1101 92-93 1.6424517868264026 1101 94-95 1.65015361325651 1101 96-97 1.6179396907971118 1101 98-99 1.603141684635613 1101 100-101 1.5492998064361352 1101 102-103 1.5670194140745792 1101 104-105 1.5298853703505486 1101 106-107 1.6426756221420789 1101 108-109 1.6562165201116024 1101 110-111 1.7574095357721369 1101 112-113 1.6340605028875927 1101 114-115 1.6858770105536323 1101 116-117 1.7931490463654072 1101 118-119 1.7265104302334962 1101 120-121 1.7685868498193713 1101 122-123 1.8101542501640928 1101 124-125 1.7923806346416455 1102 1 0.687135357645829 1102 2 0.5486825339933041 1102 3 0.7046487395938357 1102 4 1.2326261147918238 1102 5 1.2689891105911784 1102 6 1.2610181941415632 1102 7 1.1334745715156487 1102 8 1.2752924592520678 1102 9 1.650844859780804 1102 10-11 1.1312953857018222 1102 12-13 1.4767626609229296 1102 14-15 1.7298767834323208 1102 16-17 1.328246277479252 1102 18-19 1.4774144586946818 1102 20-21 1.4483530333532357 1102 22-23 1.41143228024821 1102 24-25 1.102740056726386 1102 26-27 1.228102422243996 1102 28-29 0.8973169501185296 1102 30-31 0.8036023325620327 1102 32-33 0.7615201061734282 1102 34-35 1.2323103466445993 1102 36-37 1.402277467283227 1102 38-39 1.3694238742764924 1102 40-41 0.9403916876445635 1102 42-43 1.0406440010128222 1102 44-45 1.3711669671249531 1102 46-47 1.1824266877884035 1102 48-49 1.231444105704643 1102 50-51 1.1700405677690533 1102 52-53 1.1950290776811379 1102 54-55 1.1108869820054785 1102 56-57 1.1447209186257936 1102 58-59 1.2837833019149976 1102 60-61 1.5505000811288738 1102 62-63 1.3207304588223607 1102 64-65 1.4659769745351028 1102 66-67 1.3860880457887 1102 68-69 1.2557760889691494 1102 70-71 1.484311860904267 1102 72-73 1.2587645193167347 1102 74-75 1.4164618150818775 1102 76-77 1.2214797914123245 1102 78-79 1.1288334035331644 1102 80-81 0.7821118995238692 1102 82-83 0.6895681317468672 1102 84-85 0.7515616731533576 1102 86-87 0.8353063123161597 1102 88-89 0.6372268983519334 1102 90-91 0.6364449413328614 1102 92-93 0.6218904508677774 1102 94-95 0.7464002176063822 1102 96-97 0.9073357225195586 1102 98-99 0.9937254314940986 1102 100-101 0.8631571085891778 1102 102-103 0.7154296946252678 1102 104-105 0.727420405953076 1102 106-107 1.0709649651570885 1102 108-109 1.1093147918292985 1102 110-111 0.9121433496443743 1102 112-113 0.7933600097852391 1102 114-115 0.8417170595980004 1102 116-117 1.1774594224712907 1102 118-119 1.2384335606905807 1102 120-121 1.1622009553930042 1102 122-123 1.2449348563687685 1102 124-125 1.212995847466999 1103 1 1.1321111198133558 1103 2 0.8909929825480063 1103 3 1.0885204350667976 1103 4 1.6079366946816194 1103 5 1.112465444046645 1103 6 1.1283628864486772 1103 7 1.1203828684623218 1103 8 1.3346510312728022 1103 9 1.9621884651223986 1103 10-11 1.52912946096669 1103 12-13 1.7070869876136534 1103 14-15 1.8922840255570463 1103 16-17 1.6958997058576522 1103 18-19 1.2002591531709932 1103 20-21 1.5785967447489 1103 22-23 1.5352587341336168 1103 24-25 1.4465817226075437 1103 26-27 1.1987473130524755 1103 28-29 1.1455652830896739 1103 30-31 1.3773659191380894 1103 32-33 1.382774383930979 1103 34-35 1.2797302825231007 1103 36-37 1.6471565029286772 1103 38-39 1.7055359571445976 1103 40-41 1.7356365289953182 1103 42-43 1.7018966577876853 1103 44-45 1.6343680766501407 1103 46-47 1.7125066247160063 1103 48-49 1.6669002427411819 1103 50-51 1.5633684097441432 1103 52-53 1.6512698413335762 1103 54-55 1.5440730136308112 1103 56-57 1.6893452115164642 1103 58-59 1.8957370238450828 1103 60-61 1.932406581584992 1103 62-63 1.7781043919219215 1103 64-65 1.6961313938427018 1103 66-67 1.6539080685928909 1103 68-69 1.579097494341422 1103 70-71 1.6871506512173369 1103 72-73 1.7371342406220869 1103 74-75 1.7353317890002273 1103 76-77 1.745917052085666 1103 78-79 1.8447612938616587 1103 80-81 1.3944009795037893 1103 82-83 1.5256685009444126 1103 84-85 1.5104122978232937 1103 86-87 1.5751169373647222 1103 88-89 1.6746527660016532 1103 90-91 1.6609751817313025 1103 92-93 1.6120265739741129 1103 94-95 1.3696740189370082 1103 96-97 1.4202355106874904 1103 98-99 1.3929941553232155 1103 100-101 1.2376428409834723 1103 102-103 1.368806761624704 1103 104-105 1.4742344650396646 1103 106-107 1.289033952760711 1103 108-109 1.6073843237044834 1103 110-111 1.7150084069762848 1103 112-113 1.666379659960608 1103 114-115 1.5733711245374842 1103 116-117 1.6307116225313223 1103 118-119 1.744253480162051 1103 120-121 1.7068643530121719 1103 122-123 1.7665197002200195 1103 124-125 1.7243360018113734 1104 1 1.1854531899662817 1104 2 1.0856556592068571 1104 3 1.1897397212237024 1104 4 1.4001531880728706 1104 5 1.1308400283077589 1104 6 1.132872054713424 1104 7 1.0052458741339976 1104 8 1.3822776916487598 1104 9 1.9092777202163873 1104 10-11 1.7238389014461006 1104 12-13 1.1241538888441127 1104 14-15 1.537792076601935 1104 16-17 1.1338965596180977 1104 18-19 0.5750455589472914 1104 20-21 -3.6888217465745186 1104 22-23 -0.8085634338484624 1104 24-25 0.692369750728691 1104 26-27 0.9219035642298508 1104 28-29 0.4609746624731912 1104 30-31 1.2851974391269714 1104 32-33 1.1515870808194464 1104 34-35 1.3588414707955643 1104 36-37 1.479723641670116 1104 38-39 1.500474256353911 1104 40-41 1.5239109080446696 1104 42-43 1.5817899567593905 1104 44-45 1.7010743758124995 1104 46-47 1.6009977834193485 1104 48-49 1.7419692572419407 1104 50-51 1.57932093102405 1104 52-53 1.615385538433518 1104 54-55 1.5183109763488751 1104 56-57 1.8398309399368067 1104 58-59 1.8550291369847471 1104 60-61 1.924826828918274 1104 62-63 1.5262990717894915 1104 64-65 1.7237876877133687 1104 66-67 1.668750678044372 1104 68-69 1.607605893180395 1104 70-71 1.839514789539038 1104 72-73 1.752441231851126 1104 74-75 1.7457100439372653 1104 76-77 1.7189034104922953 1104 78-79 1.6602005869441534 1104 80-81 1.4910486004843477 1104 82-83 1.4564356301816233 1104 84-85 1.384443439498547 1104 86-87 1.32380962352212 1104 88-89 1.3782983939346884 1104 90-91 1.475136970540209 1104 92-93 1.1565109706668721 1104 94-95 1.308635286565977 1104 96-97 1.3483626802589725 1104 98-99 1.2382610035041886 1104 100-101 0.8415484530395254 1104 102-103 1.2342092589641815 1104 104-105 1.3789353839014105 1104 106-107 1.4612008083443797 1104 108-109 1.4971961532850067 1104 110-111 1.5006285889538091 1104 112-113 1.5149802194982414 1104 114-115 1.4108462114434062 1104 116-117 1.4016504829064758 1104 118-119 1.431977844737343 1104 120-121 1.3495919068042923 1104 122-123 1.3182604408415521 1104 124-125 1.5456705276536837 1105 1 1.0712917432785822 1105 2 0.7033839333664886 1105 3 0.7727271093170387 1105 4 1.2713932350502688 1105 5 0.9649590525134712 1105 6 0.9950910516089415 1105 7 0.9775585459686944 1105 8 1.0792784916541613 1105 9 1.5274443085552214 1105 10-11 1.169605965735073 1105 12-13 1.3336126740904284 1105 14-15 1.626402373607558 1105 16-17 1.4359927860823518 1105 18-19 1.0219827155794725 1105 20-21 1.1607075626705168 1105 22-23 1.104569200099366 1105 24-25 0.9992553943470313 1105 26-27 0.8881903328738865 1105 28-29 0.5193131023951025 1105 30-31 0.8693147745396104 1105 32-33 0.8586815557640293 1105 34-35 0.8835489959609966 1105 36-37 1.0670713004721541 1105 38-39 0.9992128726823175 1105 40-41 1.0480328562749612 1105 42-43 1.316111346626439 1105 44-45 1.4838876734656523 1105 46-47 1.694355132998549 1105 48-49 1.3639760645255627 1105 50-51 1.4991906913022817 1105 52-53 1.4988154868143546 1105 54-55 1.4782843609700507 1105 56-57 1.6907310666445348 1105 58-59 1.4649285524693418 1105 60-61 1.856827817103479 1105 62-63 1.7384861746215492 1105 64-65 1.4350926615467259 1105 66-67 1.6310945491248035 1105 68-69 1.209629742798679 1105 70-71 1.279326204429175 1105 72-73 1.2763051324999104 1105 74-75 1.6372061505454631 1105 76-77 1.5354840773822076 1105 78-79 1.3120436392011996 1105 80-81 1.3430873597329054 1105 82-83 1.3418216834529133 1105 84-85 1.3200429079629927 1105 86-87 1.3379420229689671 1105 88-89 1.452045960866382 1105 90-91 1.5118333824821946 1105 92-93 1.5176232773362557 1105 94-95 1.3660684368984768 1105 96-97 1.4512671148365754 1105 98-99 1.213896930598505 1105 100-101 1.3441448517606744 1105 102-103 1.3921342823419032 1105 104-105 1.496178057675806 1105 106-107 1.5533928907034387 1105 108-109 1.6137274109147306 1105 110-111 1.4434931487144063 1105 112-113 1.469929203472077 1105 114-115 1.3708088842913781 1105 116-117 1.532459955202068 1105 118-119 1.6130476324222798 1105 120-121 1.6271762185921617 1105 122-123 1.7182415263591402 1105 124-125 1.412348227768426 1106 1 -0.5745106739377839 1106 2 -0.3635033001275154 1106 3 -0.24236554345489836 1106 4 -0.6859607080474142 1106 5 -1.0703459521968561 1106 6 -0.44815533918254147 1106 7 -0.3861479178157019 1106 8 -0.11714087548569196 1106 9 -0.14219599106275638 1106 10-11 -2.578454565644723 1106 12-13 -0.8839072921089297 1106 14-15 -0.44213293522031094 1106 16-17 -0.7239316918359684 1106 18-19 -3.3219346885156185 1106 20-21 -5.5868104723437355 1106 22-23 -6.171525669124112 1106 24-25 -6.019523763362173 1106 26-27 -5.196904349112863 1106 28-29 -2.2045041937692886 1106 30-31 -3.922661102311558 1106 32-33 -4.333469892281702 1106 34-35 -3.7827631953067176 1106 36-37 -6.111883095130626 1106 38-39 -6.409085243480913 1106 40-41 -2.8077505529823767 1106 42-43 -1.4382075969270787 1106 44-45 -0.606896946458491 1106 46-47 -0.4279635831260613 1106 48-49 -0.24461839542391672 1106 50-51 -0.35827784851591105 1106 52-53 -0.5599912564527223 1106 54-55 -0.4811829064226245 1106 56-57 -0.3796584739784805 1106 58-59 -0.17446772181384418 1106 60-61 0.037324023844195864 1106 62-63 -0.3302484285885292 1106 64-65 -0.4816795340931108 1106 66-67 -0.817232426982418 1106 68-69 -0.6528912478110591 1106 70-71 -0.38440457072108103 1106 72-73 -0.30863917192543866 1106 74-75 -0.3706591644876873 1106 76-77 -0.45049551871581883 1106 78-79 -0.4418680889742035 1106 80-81 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-4.1165342389883115 2216 68-69 -3.744796418757222 2216 70-71 -5.7026150201207955 2216 72-73 -6.146090651732409 2216 74-75 -4.258426539656842 2216 76-77 -3.73519091107433 2216 78-79 -4.074842522143555 2216 80-81 -4.071852781870632 2216 82-83 -4.267121524183683 2216 84-85 -4.001248648740564 2216 86-87 -4.291816444970209 2216 88-89 -4.436023329184163 2216 90-91 -4.46689632338496 2216 92-93 -4.176818838556237 2216 94-95 -6.936291004636772 2216 96-97 -8.533746625266279 2216 98-99 -5.998629260980589 2216 100-101 -5.113494939917992 2216 102-103 -5.924762182459375 2216 104-105 -8.141840974547549 2216 106-107 -5.367011064801485 2216 108-109 -7.302777770725594 2216 110-111 -5.166069988803862 2216 112-113 -4.542583282268758 2216 114-115 -4.880618569502815 2216 116-117 -4.364064540557363 2216 118-119 -4.5974732838908 2216 120-121 -5.224841067803736 2216 122-123 -7.790554917679646 2216 124-125 -6.679489927025681 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 583553.0 3 73055.0 4 40106.0 5 32090.0 6 31086.0 7 33884.0 8 36245.0 9 38043.0 10 38350.0 11 38688.0 12 38120.0 13 37309.0 14 36326.0 15 31005.0 16 26386.0 17 23327.0 18 21235.0 19 20042.0 20 20324.0 21 22880.0 22 28220.0 23 38159.0 24 49899.0 25 62018.0 26 71812.0 27 80450.0 28 89309.0 29 97930.0 30 107818.0 31 118920.0 32 134697.0 33 156548.0 34 183629.0 35 217920.0 36 255064.0 37 217555.0 38 47980.0 39 92.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.733050782099674 18.453560834923785 14.191840829471705 23.621547553504836 2 15.00337845914581 19.87760792509761 40.826634085821674 24.29237952993491 3 19.027559302837727 25.076731894544768 28.48675517523438 27.408953627383127 4 11.629682813961068 16.714564028085242 37.941443351904965 33.71430980604872 5 13.998667812377388 37.67422821059891 33.884321183702504 14.442782793321191 6 31.773154022619813 36.62913468901289 17.521034102683846 14.076677185683446 7 28.3864286877807 32.29010547792108 20.699672515929237 18.623793318368982 8 25.534700921571744 35.75170707439806 19.899327107341442 18.814264896688744 9 25.860855509362047 16.86505066260779 20.11192316642871 37.16217066160146 10-11 24.459951846778168 27.09758602987023 27.217536419748328 21.22492570360328 12-13 25.625758877987987 24.316502712856135 27.589069200867083 22.468669208288794 14-15 22.388923396259052 25.685628248170016 25.608371924803635 26.317076430767294 16-17 22.40419309196026 29.044251381071675 25.782819589507145 22.768735937460928 18-19 22.200060294840828 27.67894900147875 28.04677269269619 22.07421801098423 20-21 22.827024621068688 26.902662725666627 28.274465786717123 21.995846866547563 22-23 22.638760373571227 26.892687280977924 27.792490204354557 22.676062141096295 24-25 22.381597429057216 26.94670036552526 27.96452247404808 22.70717973136944 26-27 22.406206232995384 27.200023397436755 27.47187708294973 22.92189328661813 28-29 22.399336839601066 27.285411964969608 27.525527728724374 22.78972346670495 30-31 22.38592204578134 26.97031374926003 27.84153810957993 22.802226095378707 32-33 22.378539647018442 27.047432516173302 27.500629352695228 23.07339848411303 34-35 22.73270165448111 26.79199809153373 27.83519129402222 22.640108959962937 36-37 22.551517923588015 26.70803058693192 27.78644745392707 22.95400403555299 38-39 22.43457023311003 26.917921122774974 27.81860103161728 22.828907612497716 40-41 22.817872529030836 26.812002111991212 27.63029647304703 22.739828885930926 42-43 22.59173922396712 26.872431245117244 27.414362256868568 23.12146727404707 44-45 22.64149814828107 26.938449391596052 27.49440580662791 22.92564665349497 46-47 22.71551008445432 27.166909228590193 27.123087702173372 22.994492984782124 48-49 22.84443426890788 27.106717389899394 27.09866641591237 22.950181925280358 50-51 22.815334365830903 27.263989941089772 26.874169429926418 23.046506263152907 52-53 22.9993848325367 27.122972479118584 26.925669151867737 22.951973536476977 54-55 22.86618593989778 27.030180053177983 26.885307872646457 23.218326134277785 56-57 22.719858887024554 27.347085810444376 26.97463271615275 22.958422586378315 58-59 22.89959954930623 27.037459005501578 26.882625409158706 23.180316036033485 60-61 22.833491215407822 26.879607984811866 27.378308780219818 22.908592019560494 62-63 22.858290727175664 27.05411735477282 27.02886421405103 23.058727704000486 64-65 22.725010586319346 26.936753955383836 27.012811922970904 23.325423535325914 66-67 22.739714352520796 27.29386524165279 26.64713373672875 23.319286669097668 68-69 22.710460571585276 27.151652877487432 26.93883994782042 23.199046603106872 70-71 22.57291220346312 26.903075925234383 27.224843255567126 23.299168615735372 72-73 22.698999792769968 26.996145647178764 26.978016950095927 23.32683760995534 74-75 22.651518820239026 27.138231855382678 26.926724682817856 23.283524641560437 76-77 22.771454426664633 26.954447499325823 27.007627070005352 23.26647100400419 78-79 22.75020940097952 27.03197583624356 27.003840774105804 23.213973988671118 80-81 22.74380162690342 27.27189821908557 26.74621674592586 23.238083408085146 82-83 22.795146395882895 27.267166786429154 26.725326700011475 23.21236011767647 84-85 22.84993930779439 27.111051012899452 26.650450631764976 23.38855904754118 86-87 22.794460557510334 27.076874261586394 26.818679108755806 23.30998607214747 88-89 22.812528647053988 27.032346818551545 26.751297107156606 23.403827427237857 90-91 22.798466058903806 27.215771749766148 26.785838671397823 23.199923519932224 92-93 22.76532968341807 27.039063894219545 26.809439989056543 23.386166433305846 94-95 22.706321149866593 27.0062766680561 26.768600087139692 23.51880209493761 96-97 22.859951912282366 26.876433559264683 26.74569547003134 23.517919058421608 98-99 22.608702351721778 26.824593832390764 26.746228263932213 23.82047555195524 100-101 22.57028750226024 26.883749086077717 26.84459783488864 23.701365576773405 102-103 22.55959198023171 26.59864240786757 27.110428101264073 23.731337510636653 104-105 22.480015585319798 26.73264431083153 27.138850073217352 23.648490030631315 106-107 22.463820526035917 26.78336088531969 27.04338832514845 23.70943026349594 108-109 22.40980241554135 26.687353038058575 26.9240644553161 23.978780091083976 110-111 22.247604833775863 26.810814533403082 27.138126008550007 23.80345462427105 112-113 22.424238993639488 26.87845093640308 26.979364980531038 23.717945089426383 114-115 22.31088257080175 26.987550426676073 26.85163798548328 23.8499290170389 116-117 22.13681440004604 26.80932599747823 27.111378903750786 23.942480698724946 118-119 22.16711132746658 26.834886186961317 26.91174269297147 24.086259792600643 120-121 22.0641623074354 26.963383715291517 27.01519446563697 23.95725951163611 122-123 22.161268634093144 26.869856009137326 26.986446845899465 23.982428510870072 124-125 22.19383930609486 26.826921246636825 26.693298854330354 24.285940592937962 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 535.0 1 562.5 2 617.5 3 623.0 4 565.0 5 517.0 6 570.0 7 695.0 8 896.5 9 1099.5 10 1155.0 11 1203.5 12 1371.0 13 1556.0 14 1697.5 15 1893.0 16 2132.0 17 2432.5 18 2796.5 19 3223.5 20 3874.5 21 4641.5 22 5526.0 23 6834.5 24 8711.5 25 11399.0 26 15058.5 27 19570.0 28 24385.5 29 29324.0 30 35070.0 31 41984.0 32 48554.5 33 54338.0 34 60385.0 35 66068.0 36 72460.0 37 79056.5 38 84304.0 39 88380.5 40 92132.5 41 96917.0 42 101811.0 43 106588.0 44 111153.0 45 114789.5 46 116551.0 47 118078.0 48 120014.5 49 120244.0 50 119413.0 51 117757.5 52 115380.0 53 113118.0 54 109134.0 55 102105.0 56 94386.0 57 86523.5 58 78863.0 59 70300.5 60 59673.0 61 49217.0 62 40212.0 63 32214.0 64 25219.5 65 19592.5 66 15165.5 67 11842.0 68 9047.5 69 6715.5 70 5043.5 71 3884.5 72 2929.0 73 2291.0 74 1792.0 75 1278.5 76 901.0 77 582.0 78 404.5 79 286.0 80 175.5 81 131.0 82 95.5 83 68.5 84 51.0 85 38.0 86 30.0 87 23.0 88 18.0 89 12.5 90 12.0 91 10.5 92 8.0 93 5.5 94 4.5 95 6.5 96 4.5 97 5.0 98 6.0 99 6.0 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10392839915046002 2 0.03490484812617568 3 0.04635112264683149 4 0.05503016596469139 5 0.0579546262131007 6 0.003742051285599014 7 0.002264098256833017 8 0.002264098256833017 9 0.00672940315225369 10-11 0.06583180139833224 12-13 0.006760848961376371 14-15 0.012546877839949636 16-17 0.023568633937449256 18-19 0.021933451863069855 20-21 0.13834583723523414 22-23 0.018867485473608475 24-25 0.01698073692624763 26-27 0.007499825475759368 28-29 0.004701148463840779 30-31 0.0020911463066582725 32-33 0.006902355102428434 34-35 0.01753103858589454 36-37 0.0027829541073572505 38-39 0.009732477923469705 40-41 0.034071534184424636 42-43 0.016288929125548652 44-45 0.11213575533147971 46-47 0.004087955185948503 48-49 0.010518623151536725 50-51 0.012751275599247061 52-53 0.01421350572345172 54-55 0.011776455516443957 56-57 0.006619342820324307 58-59 0.01671344754870484 60-61 0.031870327545836984 62-63 0.008537537176807835 64-65 0.00825452489470371 66-67 0.00992115277820579 68-69 0.008993501409086706 70-71 0.004292352945245928 72-73 0.001037711701048466 74-75 0.0012578323649072318 76-77 0.008851995268034643 78-79 0.02411893559709617 80-81 0.003348978671565504 82-83 0.0053143417417330545 84-85 0.0029559060575319947 86-87 0.0036162680491082914 88-89 0.006587897011201627 90-91 0.005047052364190268 92-93 0.0028772915347252924 94-95 0.003852111617528397 96-97 0.0016351820743794013 98-99 0.003333255767004164 100-101 0.0031131351031453984 102-103 0.0010062658919257853 104-105 0.004984160745944905 106-107 7.861452280670197E-4 108-109 0.001305001078591253 110-111 0.0023741585887624 112-113 0.004402413277175311 114-115 0.0026886166799892077 116-117 0.005267173028049033 118-119 0.0038363887129670565 120-121 0.007295427716461944 122-123 0.004716871368402119 124-125 0.05391383974083622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3180074.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.99034390957007 #Duplication Level Percentage of deduplicated Percentage of total 1 86.95934519982202 63.471925122860306 2 7.531758878521047 10.99491341574418 3 2.4231973885140783 5.306100322452441 4 1.0559402619919884 3.08293771483027 5 0.5717161559070101 2.0864879419155025 6 0.3469789585412425 1.5195668107985845 7 0.22857579938449132 1.167867834453524 8 0.14674771310956056 0.8568932838247799 9 0.11139858485779404 0.731791891782883 >10 0.5820146940226162 7.503282475060684 >50 0.030283635831903313 1.4901137439472223 >100 0.011776503911886487 1.5204126756229759 >500 1.3311279252282765E-4 0.058198531507983306 >1k 1.3311279184667813E-4 0.20950823519871037 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.144580912268079E-5 0.0 0.0 0.0 0.0 3 3.144580912268079E-5 0.0 0.0 3.144580912268079E-5 0.0 4 3.144580912268079E-5 0.0 0.0 3.144580912268079E-5 0.0 5 3.144580912268079E-5 0.0 0.0 3.144580912268079E-5 0.0 6 3.144580912268079E-5 0.0 0.0 3.144580912268079E-5 3.144580912268079E-5 7 9.433742736804238E-5 0.0 0.0 3.144580912268079E-5 3.144580912268079E-5 8 1.2578323649072316E-4 0.0 0.0 6.289161824536158E-5 3.144580912268079E-5 9 1.2578323649072316E-4 0.0 0.0 6.289161824536158E-5 3.144580912268079E-5 10-11 1.2578323649072316E-4 0.0 0.0 7.861452280670197E-5 3.144580912268079E-5 12-13 1.5722904561340397E-4 0.0 0.0 9.433742736804238E-5 3.144580912268079E-5 14-15 2.8301228210412713E-4 0.0 0.0 9.433742736804238E-5 4.716871368402119E-5 16-17 2.9873518666546754E-4 0.0 0.0 1.1006033192938277E-4 6.289161824536158E-5 18-19 3.6162680491082916E-4 0.0 0.0 1.2578323649072316E-4 6.289161824536158E-5 20-21 4.087955185948503E-4 0.0 0.0 2.0439775929742516E-4 6.289161824536158E-5 22-23 4.402413277175311E-4 0.0 0.0 2.3584356842010592E-4 7.861452280670197E-5 24-25 6.289161824536159E-4 0.0 0.0 3.616268049108291E-4 9.433742736804238E-5 26-27 6.289161824536159E-4 0.0 0.0 5.660245642082543E-4 9.433742736804238E-5 28-29 8.804826554350623E-4 0.0 0.0 0.0010219887964871258 9.433742736804238E-5 30-31 9.748200828031046E-4 0.0 0.0 0.002499941825253123 1.1006033192938277E-4 32-33 9.90542987364445E-4 0.0 0.0 0.006179101492606776 1.2578323649072316E-4 34-35 0.001069157510171147 0.0 0.0 0.010974587383815597 1.2578323649072316E-4 36-37 0.0011634949375391892 0.0 0.0 0.018521581573258984 1.2578323649072316E-4 38-39 0.0014465072196433164 0.0 0.0 0.032813701819517406 1.2578323649072316E-4 40-41 0.0014465072196433164 0.0 0.0 0.04954287227278359 1.2578323649072316E-4 42-43 0.0014936759333273376 0.0 0.0 0.06658650081727657 1.4150614105206357E-4 44-45 0.0015408446470113588 0.0 0.0 0.08636591475544281 1.5722904561340397E-4 46-47 0.0017609653108701245 3.144580912268079E-5 0.0 0.11105087491674723 1.8867485473608476E-4 48-49 0.0017924111199928052 4.716871368402119E-5 0.0 0.13963511540926404 1.8867485473608476E-4 50-51 0.001823856929115486 6.289161824536158E-5 0.0 0.1737066495936887 1.8867485473608476E-4 52-53 0.001823856929115486 6.289161824536158E-5 0.0 0.20658324303145148 1.8867485473608476E-4 54-55 0.001823856929115486 9.433742736804238E-5 0.0 0.24219562186288748 2.2012066385876554E-4 56-57 0.0019181943564835285 9.433742736804238E-5 0.0 0.2855593926430643 2.2012066385876554E-4 58-59 0.0019181943564835285 9.433742736804238E-5 0.0 0.3363600972807551 2.2012066385876554E-4 60-61 0.0019339172610448687 9.433742736804238E-5 0.0 0.394739241917012 2.2012066385876554E-4 62-63 0.0019496401656062092 9.433742736804238E-5 0.0 0.45500513510062973 2.515664729814463E-4 64-65 0.0019496401656062092 9.433742736804238E-5 0.0 0.518478500814761 2.8301228210412713E-4 66-67 0.00198108597472889 9.433742736804238E-5 3.144580912268079E-5 0.5906623556558747 2.8301228210412713E-4 68-69 0.00198108597472889 9.433742736804238E-5 3.144580912268079E-5 0.676682366510968 2.8301228210412713E-4 70-71 0.002059700497535592 9.433742736804238E-5 3.144580912268079E-5 0.77641275014355 2.8301228210412713E-4 72-73 0.0021540379249036343 9.433742736804238E-5 3.144580912268079E-5 0.8824637414097911 3.1445809122680794E-4 74-75 0.002232652447710336 9.433742736804238E-5 3.144580912268079E-5 0.9899455169911141 3.1445809122680794E-4 76-77 0.0022483753522716766 9.433742736804238E-5 3.144580912268079E-5 1.1131816429429002 3.1445809122680794E-4 78-79 0.002264098256833017 9.433742736804238E-5 3.144580912268079E-5 1.2535400119619857 3.1445809122680794E-4 80-81 0.002641447966305187 9.433742736804238E-5 3.144580912268079E-5 1.4156903267030891 3.1445809122680794E-4 82-83 0.002704339584550548 9.433742736804238E-5 3.144580912268079E-5 1.5903875192841426 3.1445809122680794E-4 84-85 0.0029401831529706542 9.433742736804238E-5 3.144580912268079E-5 1.7694085106195643 3.1445809122680794E-4 86-87 0.003097412198584058 9.433742736804238E-5 3.144580912268079E-5 1.955677760957764 3.459039003494887E-4 88-89 0.0031131351031453984 9.433742736804238E-5 3.144580912268079E-5 2.166742031789197 3.459039003494887E-4 90-91 0.0032860870533201426 9.433742736804238E-5 3.144580912268079E-5 2.4021453588815858 3.459039003494887E-4 92-93 0.0034275931943722063 9.433742736804238E-5 3.144580912268079E-5 2.6589003903682746 3.773497094721695E-4 94-95 0.0034590390034948872 9.433742736804238E-5 3.144580912268079E-5 2.9187056653398633 3.930726140335099E-4 96-97 0.0034590390034948872 9.433742736804238E-5 3.144580912268079E-5 3.183872450766869 4.087955185948503E-4 98-99 0.003600545144546951 9.433742736804238E-5 3.144580912268079E-5 3.4705481696337883 4.087955185948503E-4 100-101 0.0037263283810376737 9.433742736804238E-5 3.144580912268079E-5 3.798323561024052 4.087955185948503E-4 102-103 0.0038206658084057165 9.433742736804238E-5 3.144580912268079E-5 4.144762040128626 4.402413277175311E-4 104-105 0.004449581990859331 9.433742736804238E-5 3.144580912268079E-5 4.5031027579861345 4.559642322788715E-4 106-107 0.004591088131911395 9.433742736804238E-5 3.144580912268079E-5 4.861537813271012 5.031329459628926E-4 108-109 0.004653979750156757 9.433742736804238E-5 3.144580912268079E-5 5.240239692535456 5.031329459628926E-4 110-111 0.0047483171775248 9.433742736804238E-5 3.144580912268079E-5 5.661393414115521 5.031329459628926E-4 112-113 0.0048112087957701615 9.433742736804238E-5 3.144580912268079E-5 6.1144489090505445 5.031329459628926E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6425 0.0 52.254436 1 GGTATCA 3840 0.0 47.90098 1 CAACGCA 7880 0.0 40.677044 5 ATCAACG 7670 0.0 40.493683 3 TCAACGC 7785 0.0 39.7965 4 AACGCAG 8000 0.0 39.37553 6 TATCAAC 8665 0.0 38.31579 2 GTACATG 9345 0.0 33.06018 1 ACGCAGA 9380 0.0 32.820747 7 CGCAGAG 9315 0.0 32.540924 8 TACATGG 9515 0.0 30.26556 2 ACATGGG 9800 0.0 26.896132 3 GCAGAGT 10405 0.0 26.55692 9 AGAGTAC 9400 0.0 22.984467 10-11 CAGAGTA 10040 0.0 22.616035 10-11 GAGTACT 5600 0.0 21.88192 12-13 GTATAGG 1255 0.0 20.870176 1 CATGGGG 5740 0.0 20.136778 4 CATGGGA 5500 0.0 19.607222 4 GAGTACA 7625 0.0 19.51719 1 >>END_MODULE