FastQCFastQC Report
Thu 26 May 2016
SRR1364487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364487_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3180074
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51180.1609396510898803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47570.14958771399659254No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51300.059.6567041
GTATCAA83250.048.441961
ATCAACG103450.037.2701073
TCAACGC103850.037.0117874
TATCAAC109250.036.491482
CAACGCA105150.036.4343835
AACGCAG108250.035.1711776
GTACATG101700.030.7375721
ACGCAGA130700.028.765797
CGCAGAG132900.028.6838958
TACATGG107750.028.261192
ACATGGG104900.027.0407243
GCAGAGT147250.025.0404119
GAGTACT87750.024.73799312-13
GTACTTT96600.022.7178914-15
CAGAGTA140950.022.61613710-11
AGAGTAC136400.022.43312310-11
GTGGTAT28500.022.3974271
AGTACTT96050.021.02137412-13
TGGTATC29900.020.9269032
Produced by FastQC (version 0.11.5)