##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364487_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3180074 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.962905894642702 31.0 28.0 33.0 25.0 34.0 2 29.120836496257635 31.0 28.0 33.0 25.0 34.0 3 29.200954757656582 31.0 28.0 34.0 25.0 34.0 4 32.619785577316755 35.0 32.0 37.0 26.0 37.0 5 31.749036343179437 35.0 32.0 37.0 20.0 37.0 6 31.798248091082158 35.0 32.0 37.0 22.0 37.0 7 31.232798041806575 35.0 31.0 37.0 17.0 37.0 8 31.35990231673854 35.0 32.0 37.0 17.0 37.0 9 31.97321949111876 37.0 30.0 39.0 11.0 39.0 10-11 32.14040019194522 37.0 31.0 39.0 16.5 39.0 12-13 31.908094434280457 37.0 30.5 39.0 13.5 39.0 14-15 32.840847571471606 37.5 31.0 40.0 14.0 41.0 16-17 32.883605695968086 38.0 31.0 40.0 14.5 41.0 18-19 32.7441325264758 38.0 31.0 40.0 11.0 41.0 20-21 32.63492783501265 37.5 31.0 40.0 10.5 41.0 22-23 32.60689405340882 37.0 31.0 40.0 10.5 41.0 24-25 32.46296359770244 37.0 30.5 40.0 10.0 41.0 26-27 32.48839759703705 37.0 31.0 40.0 10.0 41.0 28-29 32.35342510897545 37.0 30.0 40.0 9.5 41.0 30-31 31.872637240517044 37.0 30.0 40.0 8.0 41.0 32-33 31.833844275321894 37.0 30.0 40.0 8.0 41.0 34-35 31.686527577660144 37.0 30.0 40.0 8.0 41.0 36-37 31.634922489225094 37.0 30.0 40.0 3.5 41.0 38-39 31.43242374234059 37.0 30.0 40.0 2.0 41.0 40-41 31.115722464320015 36.0 29.5 40.0 2.0 41.0 42-43 31.02082907504668 36.0 29.0 40.0 2.0 41.0 44-45 30.947230787711227 36.0 29.0 40.0 2.0 41.0 46-47 30.80904280843779 36.0 29.0 40.0 2.0 41.0 48-49 30.54475839241477 36.0 28.0 40.0 2.0 41.0 50-51 30.234024271133315 36.0 27.5 40.0 2.0 41.0 52-53 30.113018281964507 35.5 28.0 40.0 2.0 41.0 54-55 29.63917176141184 35.0 26.0 39.5 2.0 41.0 56-57 29.655267613269377 35.0 27.0 39.0 2.0 41.0 58-59 29.432291984400365 35.0 26.0 39.0 2.0 41.0 60-61 29.16047362419868 35.0 26.0 39.0 2.0 41.0 62-63 28.89388171470224 34.0 26.0 39.0 2.0 40.0 64-65 28.404872968364888 34.0 25.0 38.0 2.0 40.0 66-67 28.05606866381097 34.0 23.5 38.0 2.0 40.0 68-69 27.864135866020728 34.0 23.5 37.5 2.0 40.0 70-71 27.926136938951736 34.0 25.5 37.0 2.0 40.0 72-73 27.72451332893511 34.0 25.0 36.5 2.0 39.0 74-75 27.366313802760565 34.0 24.0 36.0 2.0 39.0 76-77 27.254189525149414 33.5 25.0 36.0 2.0 39.0 78-79 26.85882781344082 33.0 23.5 35.0 2.0 37.5 80-81 26.65282333052627 33.0 23.5 35.0 2.0 37.0 82-83 26.495614410230708 33.0 23.5 35.0 2.0 37.0 84-85 26.09798451231009 33.0 21.5 35.0 2.0 36.0 86-87 25.97069140529434 33.0 21.5 35.0 2.0 36.0 88-89 25.90693738573379 33.0 21.5 35.0 2.0 36.0 90-91 25.737061307378383 33.0 20.0 35.0 2.0 35.0 92-93 25.761081503134832 33.0 22.0 35.0 2.0 35.0 94-95 25.750340715341842 33.0 22.5 35.0 2.0 35.0 96-97 25.627739165818156 33.0 20.0 35.0 2.0 35.0 98-99 25.516692064398498 33.0 20.0 35.0 2.0 35.0 100-101 25.43008228739331 33.0 20.0 35.0 2.0 35.0 102-103 24.271908924131957 30.5 18.5 34.0 2.0 35.0 104-105 25.211852460037093 32.0 19.5 35.0 2.0 35.0 106-107 25.347354652753364 33.0 19.0 35.0 2.0 35.0 108-109 25.405729394976344 33.0 19.0 35.0 2.0 35.0 110-111 25.34142523098519 33.0 19.0 35.0 2.0 35.0 112-113 25.2738679980403 33.0 19.0 35.0 2.0 35.0 114-115 25.217955619900668 33.0 18.5 35.0 2.0 35.0 116-117 25.130566144058285 33.0 18.0 35.0 2.0 35.0 118-119 25.005060888520205 33.0 15.0 35.0 2.0 35.0 120-121 24.842349737773397 33.0 4.5 35.0 2.0 35.0 122-123 24.550590489403703 33.0 2.0 35.0 2.0 35.0 124-125 23.77064810441518 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7547249490398045 1101 2 0.6938314874829707 1101 3 0.7270610117210765 1101 4 0.79108206183178 1101 5 0.9008035043427043 1101 6 0.9614197115896097 1101 7 1.3560910480264212 1101 8 1.2473788520194304 1101 9 1.8732859053896185 1101 10-11 1.531780145836045 1101 12-13 1.7171333616849687 1101 14-15 1.703217457591606 1101 16-17 1.6454895508331617 1101 18-19 1.642828200877382 1101 20-21 1.8011084600399059 1101 22-23 1.8547861023356518 1101 24-25 1.9718918541530925 1101 26-27 1.775907514297515 1101 28-29 1.8077303581435018 1101 30-31 2.295592750079411 1101 32-33 2.1133619844834826 1101 34-35 2.140299403363734 1101 36-37 1.9411542018609573 1101 38-39 2.0372488601140546 1101 40-41 2.2746511417943296 1101 42-43 2.2447199977770538 1101 44-45 2.178176864382518 1101 46-47 1.9700647941462037 1101 48-49 1.963305141291361 1101 50-51 2.2140195945934167 1101 52-53 2.1738953186312457 1101 54-55 2.584594740540485 1101 56-57 2.1608587815362235 1101 58-59 2.1955646529705035 1101 60-61 2.3560227467179082 1101 62-63 2.2823570763265053 1101 64-65 2.5323217519063057 1101 66-67 2.6013983079891823 1101 68-69 2.5872722533508714 1101 70-71 2.2091581540161265 1101 72-73 2.3392681242796094 1101 74-75 2.4003936566344635 1101 76-77 2.238705125144879 1101 78-79 2.374810909671556 1101 80-81 2.396139991888493 1101 82-83 2.224175580535757 1101 84-85 2.446263110972211 1101 86-87 2.2592342191711268 1101 88-89 2.152020217030259 1101 90-91 2.233383127872763 1101 92-93 2.105242385269353 1101 94-95 1.9582987553626623 1101 96-97 1.8778674896477163 1101 98-99 1.9531147961959796 1101 100-101 2.0647680818712075 1101 102-103 1.8449933095392197 1101 104-105 1.8948249063162024 1101 106-107 1.984860618940143 1101 108-109 1.9217231944779414 1101 110-111 1.9594493632130465 1101 112-113 1.7925736267034438 1101 114-115 1.8885579628099336 1101 116-117 1.8504608227231074 1101 118-119 1.7651694702828848 1101 120-121 1.6822054058872986 1101 122-123 1.7243979189837155 1101 124-125 1.552002970619938 1102 1 0.2646911528398057 1102 2 -1.9256828856764976 1102 3 -0.2743494830859454 1102 4 0.2008623943513541 1102 5 0.6950082614589093 1102 6 0.22825079783795132 1102 7 1.0919974383389075 1102 8 0.7660059380863515 1102 9 1.5229541208532957 1102 10-11 1.228506363773839 1102 12-13 1.4060390279048782 1102 14-15 1.3459162038010675 1102 16-17 1.3582537546988505 1102 18-19 1.4385044454799285 1102 20-21 1.62356866231665 1102 22-23 1.5149860332106115 1102 24-25 1.6672126209092752 1102 26-27 1.3430032540370647 1102 28-29 1.2814419268908495 1102 30-31 1.687723795196792 1102 32-33 1.3998793181337703 1102 34-35 1.7493189304046055 1102 36-37 1.0558817835324952 1102 38-39 1.0720359174675025 1102 40-41 1.190214440466093 1102 42-43 1.1434015002613336 1102 44-45 0.9707471349867056 1102 46-47 0.6343345006463004 1102 48-49 0.6646496779894058 1102 50-51 0.8849967755117731 1102 52-53 0.6644613932518837 1102 54-55 1.0854754591906364 1102 56-57 0.69476164052897 1102 58-59 0.7552743356365426 1102 60-61 0.7152148517629442 1102 62-63 1.0008568597242942 1102 64-65 1.0805885432691156 1102 66-67 1.1877115507509934 1102 68-69 1.1945477416441896 1102 70-71 0.603043246936128 1102 72-73 0.9942112290572105 1102 74-75 1.1878303376318051 1102 76-77 1.0693401058539855 1102 78-79 0.8896635263356956 1102 80-81 0.9772951306781152 1102 82-83 0.6396461390566692 1102 84-85 0.7142743273695444 1102 86-87 0.8075853055445776 1102 88-89 0.8633947652543554 1102 90-91 0.6497552833071367 1102 92-93 0.7157701908935046 1102 94-95 0.7131984224051919 1102 96-97 0.8512360136717909 1102 98-99 0.6118962562981665 1102 100-101 0.4768433421457381 1102 102-103 0.35236231994081635 1102 104-105 0.6546067245548919 1102 106-107 0.8985082001112339 1102 108-109 0.7819658202284252 1102 110-111 0.7470706252610597 1102 112-113 0.7131409055385127 1102 114-115 1.0455751314672028 1102 116-117 0.6167876246267099 1102 118-119 0.13066892213072734 1102 120-121 0.5058403819046653 1102 122-123 0.7705262913706079 1102 124-125 0.92882375848896 1103 1 0.5505775207697461 1103 2 0.479860165218021 1103 3 0.3466018957156223 1103 4 0.339231173041469 1103 5 0.6585289333266573 1103 6 0.6269600459977802 1103 7 0.9684028323067224 1103 8 0.8420332999587075 1103 9 1.4597705029540187 1103 10-11 1.20177730570677 1103 12-13 1.1614165689727294 1103 14-15 0.7961673969587082 1103 16-17 1.0047380956510636 1103 18-19 0.9983357055198638 1103 20-21 1.3314102745594525 1103 22-23 1.0949246172920937 1103 24-25 1.4536823454092627 1103 26-27 0.7951201943316519 1103 28-29 1.0541976053588797 1103 30-31 1.6936515041014282 1103 32-33 1.3638174620651995 1103 34-35 1.4701087039363685 1103 36-37 1.4393988348111613 1103 38-39 1.4832456832769196 1103 40-41 1.5482244022132718 1103 42-43 1.5461468572896777 1103 44-45 1.399977712478556 1103 46-47 1.3564451187139852 1103 48-49 1.3939445031894913 1103 50-51 1.5923996969879823 1103 52-53 1.5330126133470188 1103 54-55 1.7457239546544479 1103 56-57 1.5856179177351528 1103 58-59 1.610168677933661 1103 60-61 1.592058759017494 1103 62-63 1.6587109349970532 1103 64-65 1.7672613836054758 1103 66-67 2.1131073012521746 1103 68-69 2.2024333491715424 1103 70-71 1.9012420038672104 1103 72-73 1.9763194846707997 1103 74-75 2.036559641015476 1103 76-77 1.8217485863937135 1103 78-79 1.9525409717616249 1103 80-81 1.885103140067983 1103 82-83 1.7663836569969256 1103 84-85 1.9739931243400832 1103 86-87 1.8810379868510978 1103 88-89 1.7655006031978928 1103 90-91 1.7881159570837752 1103 92-93 1.600979492888893 1103 94-95 1.5037292804335323 1103 96-97 1.432167084833722 1103 98-99 1.611330273034124 1103 100-101 1.5541473363496365 1103 102-103 1.4475925487833408 1103 104-105 1.4074292261610815 1103 106-107 1.4733101227308047 1103 108-109 1.4465660429064826 1103 110-111 1.4555811393649876 1103 112-113 1.3630576674855703 1103 114-115 1.1816701171090855 1103 116-117 1.3203156875445003 1103 118-119 1.3698640075951687 1103 120-121 1.3061089878082726 1103 122-123 1.4921850062823445 1103 124-125 1.2416203717000087 1104 1 0.4970021237647906 1104 2 0.3734546009670865 1104 3 0.4032952846556199 1104 4 0.2692079369131406 1104 5 0.6168612332489758 1104 6 0.7490960885782307 1104 7 1.1357571038409375 1104 8 0.8035589440451432 1104 9 1.3479126937462418 1104 10-11 0.9630823457295961 1104 12-13 1.1226262638132418 1104 14-15 0.9466198227230294 1104 16-17 0.7345758176206516 1104 18-19 0.6914655701641621 1104 20-21 1.1469491537537309 1104 22-23 1.1003501671125022 1104 24-25 1.2687671990243032 1104 26-27 1.125995031817915 1104 28-29 1.1917238484765988 1104 30-31 1.679840103157705 1104 32-33 1.5945256916077781 1104 34-35 1.5209034900922305 1104 36-37 1.4301102715566607 1104 38-39 1.6070589566667337 1104 40-41 1.693466789318876 1104 42-43 1.5683860453334404 1104 44-45 1.3389417821502114 1104 46-47 1.3444309630972455 1104 48-49 1.0784895906122784 1104 50-51 1.3907023596290458 1104 52-53 1.2936046471017875 1104 54-55 1.6760994037622545 1104 56-57 1.536533002399441 1104 58-59 1.6369726396433677 1104 60-61 1.7429986644788436 1104 62-63 1.780923162846289 1104 64-65 1.9171972679462428 1104 66-67 1.936078068563166 1104 68-69 2.0377017517289 1104 70-71 1.7975451439914956 1104 72-73 1.944239768828222 1104 74-75 2.0155445291070038 1104 76-77 1.872059679396731 1104 78-79 1.8868549758598014 1104 80-81 1.7934377781813282 1104 82-83 1.6823127017029478 1104 84-85 1.9491284980274983 1104 86-87 1.850588321477474 1104 88-89 1.7291894053076042 1104 90-91 1.761616471391136 1104 92-93 1.5200969781843199 1104 94-95 1.5340486508221112 1104 96-97 1.5324944694430478 1104 98-99 1.6016670690989976 1104 100-101 1.676102098127732 1104 102-103 1.5946109576358296 1104 104-105 1.5309437980136913 1104 106-107 1.6138039373162307 1104 108-109 1.5591142527135986 1104 110-111 1.572993032635182 1104 112-113 1.528256713806556 1104 114-115 1.4581099173991703 1104 116-117 1.437346332614485 1104 118-119 1.480153922456612 1104 120-121 1.4647436054174854 1104 122-123 1.603340411545755 1104 124-125 1.3468800519528585 1105 1 0.19550450206794778 1105 2 0.3088871201734875 1105 3 0.17246448531608394 1105 4 0.3905742573611448 1105 5 0.49543585329465856 1105 6 0.5791533241880238 1105 7 0.7794456981185363 1105 8 0.7727704127836503 1105 9 1.3769111043507074 1105 10-11 0.9963644040884887 1105 12-13 1.114973425627607 1105 14-15 1.002514627320025 1105 16-17 0.9250115551841418 1105 18-19 1.0870309925602584 1105 20-21 1.3074665227492304 1105 22-23 1.222682284193148 1105 24-25 1.464687074960743 1105 26-27 1.1702228818492273 1105 28-29 1.2521245459963808 1105 30-31 1.5705268436230178 1105 32-33 1.5031460634703606 1105 34-35 1.6066661924699517 1105 36-37 1.2872857603174985 1105 38-39 1.3756033038903155 1105 40-41 1.5459282293479077 1105 42-43 1.5039869613348031 1105 44-45 1.3636023106169866 1105 46-47 1.2901199568950332 1105 48-49 1.4310015979310258 1105 50-51 1.584244318416708 1105 52-53 1.5139523840708193 1105 54-55 1.7590747290479207 1105 56-57 1.5353205454757273 1105 58-59 1.4807474106189034 1105 60-61 1.3338201223801285 1105 62-63 1.1303873329299492 1105 64-65 1.5543088334767212 1105 66-67 1.7275666479793834 1105 68-69 1.7178971306120374 1105 70-71 1.6257372765318365 1105 72-73 1.8346575569339088 1105 74-75 1.8736152712012775 1105 76-77 1.6683306312873576 1105 78-79 1.8535775496190503 1105 80-81 1.7705897589425987 1105 82-83 1.59299349761665 1105 84-85 1.9774711744074622 1105 86-87 1.9017469782790144 1105 88-89 1.7429068441708822 1105 90-91 1.7933355758868998 1105 92-93 1.5241581877654689 1105 94-95 1.4258456521046305 1105 96-97 1.5811450272755252 1105 98-99 1.630264511976776 1105 100-101 1.6354232226130136 1105 102-103 1.4781196520250788 1105 104-105 1.0335996422510014 1105 106-107 1.2592541840247726 1105 108-109 1.3209375895178042 1105 110-111 1.4576174884819295 1105 112-113 1.4081794732365367 1105 114-115 1.3086968936199987 1105 116-117 1.337692583445616 1105 118-119 1.404482289451618 1105 120-121 1.4605613817498693 1105 122-123 1.4853151993127192 1105 124-125 1.3437965088070953 1106 1 0.49079388084393116 1106 2 0.3296488305565006 1106 3 0.4151134831094332 1106 4 0.36529200534442907 1106 5 0.44772773869009796 1106 6 0.7677818788675204 1106 7 0.9466433916757282 1106 8 0.5191403239393324 1106 9 1.3249953589181551 1106 10-11 0.8500097855661792 1106 12-13 0.7500277683923748 1106 14-15 0.8714997944459597 1106 16-17 0.769996992872052 1106 18-19 0.9042586341911232 1106 20-21 0.8145385814415746 1106 22-23 0.7456589756711338 1106 24-25 0.5021626533114656 1106 26-27 0.5176652379342741 1106 28-29 0.5798494040738831 1106 30-31 1.0872940397481052 1106 32-33 0.7691694592388103 1106 34-35 0.7535198802002654 1106 36-37 0.87289745094753 1106 38-39 0.951810025002672 1106 40-41 1.1484254379521701 1106 42-43 1.215124213699525 1106 44-45 1.1866019410450086 1106 46-47 1.0767973284050498 1106 48-49 0.9779265532539902 1106 50-51 1.2930180746893818 1106 52-53 1.0280751309806817 1106 54-55 1.5159034290074374 1106 56-57 1.1187412722875365 1106 58-59 1.1687288712418429 1106 60-61 1.3696731131192585 1106 62-63 1.3367836728930271 1106 64-65 1.483508352346945 1106 66-67 1.6283404944393851 1106 68-69 1.5186817603391525 1106 70-71 1.3352049850581622 1106 72-73 1.3136047963685122 1106 74-75 1.6194402352488844 1106 76-77 1.179082332837897 1106 78-79 1.0500140719565856 1106 80-81 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-4.153902858408575 2216 94-95 -3.9231888919789952 2216 96-97 -4.210462165622481 2216 98-99 -4.051324491051286 2216 100-101 -4.410472406687774 2216 102-103 -4.363924507074515 2216 104-105 -4.1890817975806485 2216 106-107 -4.30464337854006 2216 108-109 -4.2743854598682205 2216 110-111 -4.0533259565990605 2216 112-113 -4.084564211474362 2216 114-115 -3.9768942141803088 2216 116-117 -3.938189530787362 2216 118-119 -4.1106642176247625 2216 120-121 -4.037622613201936 2216 122-123 -4.132438084115311 2216 124-125 -4.032761970022666 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 157021.0 3 52051.0 4 24884.0 5 20420.0 6 20691.0 7 24075.0 8 27028.0 9 28463.0 10 30098.0 11 32484.0 12 34665.0 13 36087.0 14 36075.0 15 30261.0 16 23189.0 17 20387.0 18 18144.0 19 17075.0 20 16935.0 21 18228.0 22 22261.0 23 30568.0 24 42041.0 25 55179.0 26 68100.0 27 77083.0 28 88010.0 29 98780.0 30 110241.0 31 125937.0 32 147902.0 33 175321.0 34 211615.0 35 265652.0 36 371461.0 37 463974.0 38 157098.0 39 590.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.70320020926976 19.623509606168042 14.19304127694844 24.48024890761376 2 16.0077495246954 20.57274911904344 38.7013665754107 24.71813478085046 3 19.38869975981691 24.62612505243589 29.1800756837734 26.805099503973807 4 13.227983262185337 17.528838014736156 36.12264358680418 33.12053513627433 5 13.655940081897466 38.119710421832956 34.01455437829434 14.20979511797524 6 32.20633508067433 37.142830189272566 16.91462766542875 13.736207064624365 7 28.077364330404315 32.09881087086934 20.810215921892013 19.013608876834333 8 25.762922498029923 36.46792496023677 19.16210125927887 18.607051282454435 9 25.546103644129037 16.321192525708522 19.792589732188624 38.340114097973824 10-11 25.04924450873414 26.89280042766623 27.105108410245123 20.952846653354506 12-13 25.794071009833957 23.935579679895223 27.683583840203895 22.586765470066926 14-15 22.589832972988354 25.431026417943464 25.494641679883156 26.48449892918502 16-17 22.577509065045206 28.657115421717023 26.03213997017863 22.73323554305914 18-19 22.453976347651626 27.207732020764002 28.22555500786551 22.112736623718856 20-21 22.972849522596572 26.486192840324374 28.327157946044963 22.21379969103409 22-23 22.732191944407802 26.299078196027093 28.294808089711545 22.673921769853564 24-25 22.590038825047028 26.56224743750287 28.149369824139452 22.698343913310644 26-27 22.456036298975025 26.752647743680708 28.111217734886473 22.680098222457797 28-29 22.410511227842434 26.746598690797846 28.295773017957504 22.547117063402215 30-31 22.438754797495005 26.522942285407915 28.20122042298144 22.83708249411565 32-33 22.292246842209863 26.608642823181363 28.24189200747656 22.857218327132216 34-35 22.78909815830441 26.626528699479863 28.016656438780558 22.56771670343517 36-37 22.718787258821482 26.431890461600194 27.98390143757829 22.865420842000034 38-39 22.577353983703432 26.696416666876306 28.085684654609857 22.640544694810405 40-41 22.70654960671668 26.43951002870105 28.100520027685334 22.753420336896934 42-43 22.71081426690727 26.543480759467357 27.81744091945883 22.92826405416654 44-45 22.789550329979814 26.668365805116473 27.714656688604528 22.82742717629919 46-47 22.845194771053563 26.928394904887227 27.31137425720905 22.915036066850163 48-49 23.010663118321006 27.004706023304177 27.036953705629756 22.947677152745054 50-51 22.867894173395996 26.995888631589743 27.0359673166817 23.100249878332562 52-53 23.138013856290048 26.99630700185405 26.935160587559025 22.93051855429688 54-55 22.902911369425762 26.820383285986377 26.94232603791861 23.334379306669252 56-57 22.843373145418905 27.19334780788251 26.942252271927185 23.021026774771393 58-59 22.998081176727332 26.94855528519148 26.93100852370102 23.12235501438017 60-61 22.98864743399053 26.869280400393198 27.179854934193354 22.962217231422915 62-63 22.8955388807863 26.92180698818322 27.168595250269334 23.014058880761144 64-65 22.754871151999957 26.809940192829117 27.021296412545187 23.41389224262574 66-67 22.63672241172999 27.11569942208169 26.887341160542512 23.360237005645807 68-69 22.607545534004807 26.961237743173104 27.158218039011466 23.27299868381062 70-71 22.69505910181468 26.864772827866602 27.123689672744643 23.316478397574073 72-73 22.761687471158048 26.92367662147039 26.887441767379787 23.427194139991776 74-75 22.703333517299676 27.05764388801701 26.790075062984887 23.448947531698423 76-77 22.801204393047225 26.90849914701928 26.91250854946109 23.377787910472403 78-79 22.775853700452213 27.080240130300925 26.86826056474111 23.275645604505755 80-81 22.69350844070001 27.106747402885723 26.855919433169355 23.343824723244914 82-83 22.775221929077034 27.12701292738839 26.845305292021877 23.252459851512704 84-85 22.709242838136106 26.96633064712665 26.72371043054672 23.60071608419052 86-87 22.701829442299573 26.92211721920997 26.986109571573557 23.3899437669169 88-89 22.726550891895503 26.935998930826017 26.79235226138159 23.545097915896886 90-91 22.658492610913342 27.105482079513244 26.89672825509821 23.339297054475207 92-93 22.71235779697796 26.96411278663285 26.916156879652213 23.407372536736975 94-95 22.619507158220166 26.889564616752082 26.921263273222763 23.56966495180499 96-97 22.770206580351086 26.819392153610185 26.853574570420257 23.556826695618472 98-99 22.402639058084812 26.755808198174 26.846356405542764 23.99519633819842 100-101 22.41273396191201 26.809136953517598 27.006509212251512 23.771619872318876 102-103 22.596699998128948 26.64303165836407 26.928186335436934 23.832082008070053 104-105 22.478890388507416 26.703910105066935 27.02492525317069 23.79227425325496 106-107 22.341015810174138 26.826731151569653 26.965250006092628 23.867003032163574 108-109 22.398236644807636 26.770477668129733 26.723293231541152 24.107992455521476 110-111 22.223127163867463 26.845556858811282 26.972299351901057 23.959016625420197 112-113 22.407897817295446 26.82788122331327 26.901779002559223 23.86244195683206 114-115 22.25772809610862 27.009449639213745 26.699928004695494 24.032894259982136 116-117 22.140381010001654 26.88056944586824 26.879012878316665 24.100036665813438 118-119 22.16040244871711 26.930149298788614 26.636127146499287 24.273321105994984 120-121 22.017363538393276 26.971251859671565 26.8753240962177 24.136060505717456 122-123 22.118297920245634 26.970754132260645 26.700254305057264 24.21069364243646 124-125 22.042363778653197 26.91203310380744 26.655241475488122 24.390361642051246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 113.0 1 102.0 2 144.0 3 225.5 4 212.0 5 181.5 6 332.5 7 754.0 8 1249.5 9 1576.0 10 1463.0 11 1177.5 12 1143.0 13 1275.0 14 1455.5 15 1656.5 16 1952.0 17 2279.0 18 2666.0 19 3172.5 20 3776.5 21 4458.0 22 5414.0 23 6749.0 24 8391.0 25 10959.5 26 14631.0 27 18930.0 28 23646.0 29 28821.0 30 34630.5 31 41375.5 32 48119.0 33 53676.0 34 59561.0 35 65506.0 36 71732.0 37 78504.5 38 83829.5 39 87544.5 40 90958.5 41 96036.5 42 101034.5 43 105611.0 44 110765.0 45 114799.0 46 116279.0 47 117666.0 48 120987.0 49 122231.0 50 121344.5 51 119798.0 52 116885.5 53 114577.5 54 111797.5 55 105625.5 56 97477.0 57 88911.5 58 81539.5 59 72952.5 60 61352.0 61 49693.5 62 39989.5 63 31691.0 64 24193.5 65 18253.0 66 13740.5 67 10618.5 68 7927.5 69 5661.5 70 4169.0 71 3211.0 72 2464.5 73 2013.0 74 1587.0 75 1091.5 76 711.5 77 397.0 78 256.0 79 156.5 80 98.5 81 68.0 82 43.0 83 27.0 84 15.0 85 9.0 86 7.0 87 6.5 88 3.5 89 3.0 90 3.5 91 2.5 92 1.5 93 2.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10417996562344146 2 2.515664729814463E-4 3 0.0 4 0.1793983410448939 5 0.0 6 1.2578323649072316E-4 7 0.037514850283358187 8 0.0 9 0.0 10-11 7.54699418944339E-4 12-13 6.76084896137637E-4 14-15 6.131932778922755E-4 16-17 0.004386690372613971 18-19 0.001619459169818061 20-21 0.0035062077171789086 22-23 0.001871025642799507 24-25 0.005282895932610374 26-27 0.004701148463840779 28-29 0.0038363887129670565 30-31 9.11928464557743E-4 32-33 6.289161824536158E-5 34-35 0.0013364468877139336 36-37 0.0012106636512232104 38-39 5.660245642082543E-4 40-41 0.0016037362652567206 42-43 9.276513691190834E-4 44-45 9.748200828031046E-4 46-47 2.2012066385876557E-4 48-49 1.5722904561340395E-5 50-51 0.04172858870579741 52-53 6.289161824536158E-5 54-55 0.13847162047172487 56-57 2.9873518666546754E-4 58-59 0.0 60-61 0.0 62-63 6.289161824536159E-4 64-65 0.06374065509167397 66-67 0.033898582234249894 68-69 0.0019024714519221878 70-71 0.0030030747712160156 72-73 0.069007828119723 74-75 0.004543919418227374 76-77 0.0015408446470113588 78-79 0.00158801336069538 80-81 1.8867485473608476E-4 82-83 1.2578323649072316E-4 84-85 3.144580912268079E-5 86-87 2.0439775929742516E-4 88-89 0.0015408446470113588 90-91 3.9307261403350986E-4 92-93 0.002185483734026315 94-95 0.004040786472264482 96-97 0.0024056043978850803 98-99 0.0 100-101 0.0013364468877139336 102-103 0.001430784315081976 104-105 1.8867485473608476E-4 106-107 4.716871368402119E-5 108-109 0.0 110-111 1.2578323649072316E-4 112-113 1.7295195017474438E-4 114-115 1.7295195017474438E-4 116-117 0.0 118-119 0.001305001078591253 120-121 0.002421327302446421 122-123 0.0011006033192938278 124-125 0.008128741658212984 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3180074.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.95480444874076 #Duplication Level Percentage of deduplicated Percentage of total 1 79.51235391446562 47.67147630200854 2 11.139857479916355 13.357759535904542 3 3.917206117538228 7.045659802872466 4 1.8432853718810618 4.420552560574138 5 0.9711603746538164 2.911286517536769 6 0.5840110897418671 2.1008562408821785 7 0.3907423816369832 1.6398818156616395 8 0.272945525864046 1.3091516502670073 9 0.20644182298719324 1.1139461214514854 >10 1.0595212831019978 11.403395323563295 >50 0.07073137331201071 2.9273047152683125 >100 0.030362704334330665 3.204163334092834 >500 0.0011681666335340952 0.45107141627760605 >1k 1.5929544967967766E-4 0.28056266389002127 >5k 5.3098483226559216E-5 0.16293199974913522 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5118 0.1609396510898803 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4757 0.14958771399659254 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 9.433742736804238E-5 0.0 0.0 0.0 0.0 3 9.433742736804238E-5 0.0 0.0 0.0 0.0 4 9.433742736804238E-5 0.0 0.0 0.0 0.0 5 9.433742736804238E-5 3.144580912268079E-5 0.0 0.0 0.0 6 1.2578323649072316E-4 3.144580912268079E-5 0.0 0.0 3.144580912268079E-5 7 2.515664729814463E-4 6.289161824536158E-5 0.0 0.0 3.144580912268079E-5 8 2.515664729814463E-4 6.289161824536158E-5 0.0 0.0 3.144580912268079E-5 9 2.515664729814463E-4 6.289161824536158E-5 0.0 6.289161824536158E-5 3.144580912268079E-5 10-11 2.8301228210412713E-4 6.289161824536158E-5 0.0 6.289161824536158E-5 3.144580912268079E-5 12-13 3.459039003494887E-4 6.289161824536158E-5 0.0 6.289161824536158E-5 3.144580912268079E-5 14-15 4.716871368402119E-4 6.289161824536158E-5 0.0 6.289161824536158E-5 7.861452280670197E-5 16-17 4.874100414015523E-4 6.289161824536158E-5 0.0 7.861452280670197E-5 9.433742736804238E-5 18-19 5.503016596469139E-4 6.289161824536158E-5 0.0 9.433742736804238E-5 1.2578323649072316E-4 20-21 5.660245642082543E-4 6.289161824536158E-5 0.0 1.4150614105206357E-4 1.2578323649072316E-4 22-23 5.817474687695946E-4 6.289161824536158E-5 0.0 1.7295195017474435E-4 1.2578323649072316E-4 24-25 7.54699418944339E-4 6.289161824536158E-5 0.0 3.1445809122680794E-4 1.7295195017474435E-4 26-27 7.54699418944339E-4 6.289161824536158E-5 0.0 4.874100414015523E-4 1.8867485473608476E-4 28-29 9.90542987364445E-4 6.289161824536158E-5 0.0 8.490368463123813E-4 1.8867485473608476E-4 30-31 0.0011006033192938278 6.289161824536158E-5 0.0 0.002468496016130442 1.8867485473608476E-4 32-33 0.0011163262238551683 6.289161824536158E-5 0.0 0.006477836679272243 1.8867485473608476E-4 34-35 0.0011792178421005297 6.289161824536158E-5 0.0 0.011745009707321275 1.8867485473608476E-4 36-37 0.0012578323649072318 6.289161824536158E-5 0.0 0.020188209456761072 1.8867485473608476E-4 38-39 0.0013836156013979548 6.289161824536158E-5 0.0 0.03580105368617208 1.8867485473608476E-4 40-41 0.0013836156013979548 6.289161824536158E-5 0.0 0.05381950231346818 1.8867485473608476E-4 42-43 0.0014465072196433164 7.861452280670197E-5 0.0 0.07226246936392046 1.8867485473608476E-4 44-45 0.0015722904561340397 1.1006033192938277E-4 0.0 0.09329971566699391 1.8867485473608476E-4 46-47 0.0016351820743794013 1.2578323649072316E-4 0.0 0.1203431115124994 1.8867485473608476E-4 48-49 0.0017295195017474436 1.2578323649072316E-4 0.0 0.1517574748260575 1.8867485473608476E-4 50-51 0.0017295195017474436 1.2578323649072316E-4 0.0 0.18870630054520743 1.8867485473608476E-4 52-53 0.0017295195017474436 1.2578323649072316E-4 0.0 0.22403566709453931 2.0439775929742516E-4 54-55 0.0017924111199928052 1.2578323649072316E-4 0.0 0.2631228078340315 2.2012066385876554E-4 56-57 0.0017924111199928052 1.2578323649072316E-4 0.0 0.31021290699524606 2.2012066385876554E-4 58-59 0.001823856929115486 1.2578323649072316E-4 0.0 0.36557325395572554 2.2012066385876554E-4 60-61 0.001823856929115486 1.2578323649072316E-4 0.0 0.42794601635056295 2.2012066385876554E-4 62-63 0.0018553027382381669 1.2578323649072316E-4 0.0 0.4907118513594338 2.2012066385876554E-4 64-65 0.001902471451922188 1.2578323649072316E-4 0.0 0.5561348572391711 2.2012066385876554E-4 66-67 0.0021068692112196133 1.2578323649072316E-4 0.0 0.6318091968929025 2.2012066385876554E-4 68-69 0.0021068692112196133 1.2578323649072316E-4 0.0 0.7217756567928922 2.6728937754278673E-4 70-71 0.0022326524477103366 1.2578323649072316E-4 0.0 0.8283297810050961 3.773497094721695E-4 72-73 0.0023112669705170385 1.2578323649072316E-4 0.0 0.9365819789099248 4.087955185948503E-4 74-75 0.0023269898750783785 1.2578323649072316E-4 0.0 1.0470982750715865 4.087955185948503E-4 76-77 0.0023269898750783785 1.5722904561340397E-4 0.0 1.1719381372886293 4.087955185948503E-4 78-79 0.0023269898750783785 1.5722904561340397E-4 0.0 1.3142775922824437 4.087955185948503E-4 80-81 0.0026886166799892077 1.5722904561340397E-4 0.0 1.4803271873547597 4.087955185948503E-4 82-83 0.0028301228210412714 1.5722904561340397E-4 0.0 1.6584834189393076 4.402413277175311E-4 84-85 0.0029401831529706542 1.5722904561340397E-4 0.0 1.841953992265589 4.402413277175311E-4 86-87 0.003050243484900037 1.5722904561340397E-4 0.0 2.0335218614409603 4.559642322788715E-4 88-89 0.003050243484900037 1.5722904561340397E-4 0.0 2.2514098728520153 4.716871368402119E-4 90-91 0.003333255767004164 1.5722904561340397E-4 0.0 2.497866401851026 4.716871368402119E-4 92-93 0.003396147385249526 1.5722904561340397E-4 0.0 2.7652186710120583 4.716871368402119E-4 94-95 0.003396147385249526 1.5722904561340397E-4 0.0 3.0363758830769347 5.345787550855735E-4 96-97 0.003396147385249526 1.5722904561340397E-4 0.0 3.3146398480035373 5.345787550855735E-4 98-99 0.0037420512855990146 1.5722904561340397E-4 0.0 3.618437809937756 5.345787550855735E-4 100-101 0.004025063567703142 1.5722904561340397E-4 0.0 3.9689485213237177 5.345787550855735E-4 102-103 0.004229461327000567 1.5722904561340397E-4 0.0 4.33975750249837 5.345787550855735E-4 104-105 0.005109943982435629 1.5722904561340397E-4 0.0 4.722390107903149 5.503016596469139E-4 106-107 0.0053300646462943945 1.5722904561340397E-4 0.0 5.105793135631435 5.817474687695946E-4 108-109 0.005408679169101096 1.5722904561340397E-4 0.0 5.513047809579274 5.974703733309351E-4 110-111 0.005503016596469139 1.5722904561340397E-4 0.0 5.9638392062574646 5.974703733309351E-4 112-113 0.0055659082147145 1.5722904561340397E-4 0.0 6.446768219859035 5.974703733309351E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5130 0.0 59.656704 1 GTATCAA 8325 0.0 48.44196 1 ATCAACG 10345 0.0 37.270107 3 TCAACGC 10385 0.0 37.011787 4 TATCAAC 10925 0.0 36.49148 2 CAACGCA 10515 0.0 36.434383 5 AACGCAG 10825 0.0 35.171177 6 GTACATG 10170 0.0 30.737572 1 ACGCAGA 13070 0.0 28.76579 7 CGCAGAG 13290 0.0 28.683895 8 TACATGG 10775 0.0 28.26119 2 ACATGGG 10490 0.0 27.040724 3 GCAGAGT 14725 0.0 25.040411 9 GAGTACT 8775 0.0 24.737993 12-13 GTACTTT 9660 0.0 22.71789 14-15 CAGAGTA 14095 0.0 22.616137 10-11 AGAGTAC 13640 0.0 22.433123 10-11 GTGGTAT 2850 0.0 22.397427 1 AGTACTT 9605 0.0 21.021374 12-13 TGGTATC 2990 0.0 20.926903 2 >>END_MODULE