FastQCFastQC Report
Thu 26 May 2016
SRR1364486_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364486_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045015
Sequences flagged as poor quality0
Sequence length125
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21880.10699188025515706No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55650.055.195931
GGTATCA29400.052.846431
ATCAACG66200.044.5815283
TCAACGC68700.043.320484
CAACGCA68750.043.2879145
AACGCAG67700.042.4459846
TATCAAC74550.041.1844562
GTACATG95350.035.960381
ACGCAGA80450.034.6836627
TACATGG97300.033.8176572
CGCAGAG78950.033.685598
ACATGGG97350.030.255233
GCAGAGT87150.028.3314959
CATGGGA69500.026.463894
CGCATAG2401.3040335E-824.7900568
ATGGGAG32900.024.0616365
AGAGTAC80600.023.32517210-11
CAGAGTA85100.023.21033110-11
GAGTACA72900.023.0273591
AGTACAT70500.022.1971842
Produced by FastQC (version 0.11.5)