##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364486_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2045015 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.73662638171358 31.0 28.0 31.0 16.0 34.0 2 27.822329420566597 31.0 28.0 31.0 16.0 34.0 3 27.93753395451867 31.0 28.0 33.0 16.0 34.0 4 30.848816756845306 35.0 30.0 37.0 16.0 37.0 5 31.464792678782306 35.0 32.0 37.0 18.0 37.0 6 31.581625562648686 35.0 32.0 37.0 18.0 37.0 7 31.35221013048804 35.0 32.0 37.0 17.0 37.0 8 31.083843394791725 35.0 32.0 37.0 13.0 37.0 9 31.748297200754028 37.0 30.0 39.0 10.0 39.0 10-11 31.950658552626756 37.0 31.0 39.0 11.5 39.0 12-13 31.815167125913504 37.0 30.5 39.0 10.0 39.0 14-15 32.60292516191813 38.0 31.0 40.0 10.0 41.0 16-17 32.89882225802745 38.0 31.5 40.0 10.5 41.0 18-19 32.76370466720293 38.0 31.0 40.0 10.0 41.0 20-21 32.511477421926 38.0 30.5 40.0 10.0 41.0 22-23 32.61197717376156 38.0 31.0 40.0 10.0 41.0 24-25 32.68572406559365 38.0 31.0 40.0 10.0 41.0 26-27 32.81974777691117 38.0 31.0 40.0 10.0 41.0 28-29 32.81194538915362 38.0 31.0 40.0 10.0 41.0 30-31 32.42975308249573 38.0 30.0 40.0 9.0 41.0 32-33 32.50623149463451 38.0 30.5 40.0 8.0 41.0 34-35 32.19807874269871 37.5 30.0 40.0 8.0 41.0 36-37 31.977878646366896 37.0 30.0 40.0 7.0 41.0 38-39 31.87276768141065 37.0 30.0 40.0 2.0 41.0 40-41 31.968760131343778 37.0 30.0 40.0 2.0 41.0 42-43 31.87869893374865 37.0 30.0 40.0 2.0 41.0 44-45 31.536957430630093 37.0 30.0 40.0 2.0 41.0 46-47 31.308897000755493 37.0 29.5 40.0 2.0 41.0 48-49 31.12326022058518 37.0 29.0 40.0 2.0 41.0 50-51 31.131237912680348 37.0 29.5 40.0 2.0 41.0 52-53 31.03113449045606 36.5 29.0 40.0 2.0 41.0 54-55 30.94712019227243 36.0 29.0 40.0 2.0 41.0 56-57 30.592316926770707 36.0 28.5 40.0 2.0 41.0 58-59 30.292137465984357 35.5 28.0 40.0 2.0 41.0 60-61 29.96549462962374 35.0 28.0 39.0 2.0 41.0 62-63 29.89244626567531 35.0 28.0 39.0 2.0 40.5 64-65 29.690476108977194 35.0 28.0 39.0 2.0 40.0 66-67 29.47768818321626 34.5 28.0 39.0 2.0 40.0 68-69 29.277721679303085 34.0 27.5 38.0 2.0 40.0 70-71 29.132369933716866 34.0 28.0 38.0 2.0 40.0 72-73 29.123022569516607 34.0 28.0 37.5 2.0 40.0 74-75 28.805084314784978 34.0 28.0 37.0 2.0 39.5 76-77 28.47871727102246 34.0 27.0 36.5 2.0 39.0 78-79 28.212616288878078 34.0 26.5 36.0 2.0 39.0 80-81 28.138163289755823 34.0 27.5 36.0 2.0 37.5 82-83 27.918720156086874 34.0 27.0 35.0 2.0 37.0 84-85 27.6906609486972 34.0 27.0 35.0 2.0 37.0 86-87 27.447120681266398 34.0 26.0 35.0 2.0 36.0 88-89 27.22869636653032 34.0 26.0 35.0 2.0 36.0 90-91 27.050377381095004 33.5 26.0 35.0 2.0 36.0 92-93 26.919659757996882 33.0 26.0 35.0 2.0 35.5 94-95 26.867736666968213 33.0 26.0 35.0 2.0 35.0 96-97 26.757212049789366 33.0 26.0 35.0 2.0 35.0 98-99 26.665371892137713 33.0 25.5 35.0 2.0 35.0 100-101 26.58392114483268 33.0 25.0 35.0 2.0 35.0 102-103 25.42991958494192 31.5 23.5 34.0 2.0 35.0 104-105 26.27403295330352 33.0 25.0 35.0 2.0 35.0 106-107 26.324036743006772 33.0 25.0 35.0 2.0 35.0 108-109 26.283266137412195 33.0 25.0 35.0 2.0 35.0 110-111 26.19291203243008 33.0 24.5 35.0 2.0 35.0 112-113 26.164315176172302 33.0 24.5 35.0 2.0 35.0 114-115 26.113553445818248 33.0 24.5 35.0 2.0 35.0 116-117 25.943044427546987 33.0 23.5 35.0 2.0 35.0 118-119 25.82385165878979 33.0 23.0 35.0 2.0 35.0 120-121 25.68236052058298 33.0 23.0 35.0 2.0 35.0 122-123 25.43209194064591 33.0 19.0 35.0 2.0 35.0 124-125 24.389497876543693 32.0 9.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6356682508728717 1101 2 1.290109569182416 1101 3 1.5494029795113597 1101 4 2.0326246878678624 1101 5 1.510629960484625 1101 6 1.3705340798761334 1101 7 1.2471700744070198 1101 8 1.7453308757719768 1101 9 2.204039947529477 1101 10-11 2.2819634538250178 1101 12-13 2.0133733136775653 1101 14-15 2.254483483148121 1101 16-17 1.843452881579175 1101 18-19 1.8228220814777885 1101 20-21 2.2105896326371663 1101 22-23 2.1306822860396437 1101 24-25 1.953743947578161 1101 26-27 1.6903563890809465 1101 28-29 1.5273767182863196 1101 30-31 1.604389987313347 1101 32-33 1.846050180939713 1101 34-35 2.05544296738357 1101 36-37 2.0747283437391637 1101 38-39 2.088059633363983 1101 40-41 1.9378049989437294 1101 42-43 1.7966183419785438 1101 44-45 1.9526870506054053 1101 46-47 1.8933940149102604 1101 48-49 2.0148024547552446 1101 50-51 1.974487299153303 1101 52-53 1.712173917818184 1101 54-55 1.8594533224268126 1101 56-57 2.0337013670614397 1101 58-59 2.069961345620353 1101 60-61 2.342057944963436 1101 62-63 1.8421780535949708 1101 64-65 1.7482133956256725 1101 66-67 1.9477762691354386 1101 68-69 1.7657254293146742 1101 70-71 1.8805884599378508 1101 72-73 1.641522008555583 1101 74-75 1.625931414351598 1101 76-77 1.8650755533631624 1101 78-79 1.7948886960430812 1101 80-81 1.6254037605778109 1101 82-83 1.69301603955293 1101 84-85 1.5376174601094057 1101 86-87 1.6057365315657535 1101 88-89 1.7247116929985609 1101 90-91 1.700031932411676 1101 92-93 1.6708228224473984 1101 94-95 1.6909815796529344 1101 96-97 1.7063824688578464 1101 98-99 1.6123599782428393 1101 100-101 1.5482640716767477 1101 102-103 1.574489727969933 1101 104-105 1.5964138351873487 1101 106-107 1.686047100931006 1101 108-109 1.6912059341558283 1101 110-111 1.7688776350814912 1101 112-113 1.705343547949596 1101 114-115 1.6173947792137398 1101 116-117 1.7844312632789503 1101 118-119 1.7261424742729226 1101 120-121 1.6878987403398398 1101 122-123 1.6422910099013421 1101 124-125 1.668207105855 1102 1 0.4129938130183426 1102 2 0.1426272482075781 1102 3 0.29774487332720057 1102 4 0.7575148114314558 1102 5 0.604642749407585 1102 6 0.5760649098958162 1102 7 0.4480238085325645 1102 8 0.6631817870210064 1102 9 0.992458926630075 1102 10-11 0.5682245291383943 1102 12-13 0.9421967377495157 1102 14-15 1.1460727423326134 1102 16-17 0.6452851345721342 1102 18-19 0.7258522679926998 1102 20-21 0.8283054345865182 1102 22-23 0.7334284760108005 1102 24-25 0.49014445782492544 1102 26-27 0.5858610766081966 1102 28-29 0.3106416425236489 1102 30-31 0.33735412869997816 1102 32-33 0.1988147949062835 1102 34-35 0.5848756827743102 1102 36-37 0.6854311007877207 1102 38-39 0.7466560204095813 1102 40-41 0.4383104750535871 1102 42-43 0.3594042565759423 1102 44-45 0.7087910537924884 1102 46-47 0.5658018477606568 1102 48-49 0.7313088593632706 1102 50-51 0.5745395271750766 1102 52-53 0.6355111566825258 1102 54-55 0.5498196911409821 1102 56-57 0.6641555728412669 1102 58-59 0.7836338727196512 1102 60-61 1.1073489889079617 1102 62-63 0.9927470257353974 1102 64-65 1.1042769194257396 1102 66-67 0.924477302338861 1102 68-69 0.7694172419759298 1102 70-71 0.9294455491886673 1102 72-73 0.8077587819959753 1102 74-75 0.9333298144723585 1102 76-77 0.8593204668273486 1102 78-79 0.7393495529742609 1102 80-81 0.4043451872252106 1102 82-83 0.4270095749556617 1102 84-85 0.3945598885949373 1102 86-87 0.5069288755857144 1102 88-89 0.33507082028149426 1102 90-91 0.28830686612979406 1102 92-93 0.3095585696623502 1102 94-95 0.5061491383257959 1102 96-97 0.6697085002366698 1102 98-99 0.7632882805971626 1102 100-101 0.63446562057403 1102 102-103 0.48643289163518233 1102 104-105 0.4331757761614945 1102 106-107 0.6838524915606499 1102 108-109 0.7003068894622402 1102 110-111 0.4955848808205623 1102 112-113 0.31068832231706267 1102 114-115 0.37626120684909026 1102 116-117 0.6020273359595656 1102 118-119 0.6744748623751882 1102 120-121 0.6884903731045711 1102 122-123 0.8747823933458072 1102 124-125 0.7213962727593071 1103 1 1.2143497204477818 1103 2 0.9030862533954078 1103 3 1.0633463530460858 1103 4 1.6469010956582828 1103 5 1.0617181121095989 1103 6 0.9895999097399759 1103 7 0.9224552019022774 1103 8 1.28659189707205 1103 9 1.8895710315287637 1103 10-11 1.5382340693558056 1103 12-13 1.6268361995709064 1103 14-15 1.8464539569531198 1103 16-17 1.609826263728337 1103 18-19 1.3442640636135152 1103 20-21 1.7353271850641505 1103 22-23 1.592924180763461 1103 24-25 1.5829757170655654 1103 26-27 1.2860014034260274 1103 28-29 1.2741624019005897 1103 30-31 1.4130864021842982 1103 32-33 1.3566171200252768 1103 34-35 1.3057528167396697 1103 36-37 1.6092476463118963 1103 38-39 1.6303306771426733 1103 40-41 1.5893561910732323 1103 42-43 1.5239378266413723 1103 44-45 1.510530002921243 1103 46-47 1.6484794926632311 1103 48-49 1.6372033380385709 1103 50-51 1.497651321828613 1103 52-53 1.499532464003991 1103 54-55 1.4398333799020513 1103 56-57 1.6459772850577288 1103 58-59 1.832231159830041 1103 60-61 1.9618166444183416 1103 62-63 1.6444860644268324 1103 64-65 1.6556260021784368 1103 66-67 1.568255866118779 1103 68-69 1.4953281571955337 1103 70-71 1.7008895253993863 1103 72-73 1.568042280142567 1103 74-75 1.520294287820473 1103 76-77 1.5719137329313426 1103 78-79 1.6906807231895797 1103 80-81 1.1121770142932732 1103 82-83 1.2707147689824794 1103 84-85 1.1850717608455845 1103 86-87 1.221528345876159 1103 88-89 1.23863154611184 1103 90-91 1.2222890632171435 1103 92-93 1.241172335232374 1103 94-95 1.0609803466948193 1103 96-97 1.1283943433493562 1103 98-99 1.1351154776325636 1103 100-101 1.0047611881883043 1103 102-103 1.074355112531876 1103 104-105 1.233249038439375 1103 106-107 1.032858901024774 1103 108-109 1.269173860570394 1103 110-111 1.4335173656011762 1103 112-113 1.2750287952295452 1103 114-115 1.207614201699954 1103 116-117 1.3698513674913677 1103 118-119 1.4192388306613992 1103 120-121 1.3158440715599014 1103 122-123 1.404322603588838 1103 124-125 1.4399603607729219 1104 1 1.0856950657178466 1104 2 0.8943091162807377 1104 3 0.9476411022775402 1104 4 1.1959538099194091 1104 5 0.7605279116044876 1104 6 0.6832375777993214 1104 7 0.6328098825980035 1104 8 1.0175961308458454 1104 9 1.5139003027324058 1104 10-11 1.364601878017428 1104 12-13 0.9412295030672091 1104 14-15 1.2904809235709465 1104 16-17 0.9419508622807911 1104 18-19 0.29366802500332057 1104 20-21 -4.350770587555623 1104 22-23 -0.9865376567449147 1104 24-25 0.5653045933370819 1104 26-27 0.6186389137453929 1104 28-29 0.22247348485640117 1104 30-31 0.9952014972971241 1104 32-33 0.7289773641544315 1104 34-35 0.9942647232995228 1104 36-37 1.1620062568220355 1104 38-39 1.1708272820345798 1104 40-41 1.1442869967861462 1104 42-43 1.200109165815391 1104 44-45 1.3101836256632495 1104 46-47 1.2412488511355129 1104 48-49 1.2272218017662944 1104 50-51 1.1504183433109105 1104 52-53 1.1939449962977058 1104 54-55 1.0675463550663231 1104 56-57 1.378357582643126 1104 58-59 1.3438188150708648 1104 60-61 1.3262156773757212 1104 62-63 1.0088610562504527 1104 64-65 1.1577259712400298 1104 66-67 1.0998509158918033 1104 68-69 1.071761291302522 1104 70-71 1.2991708446096837 1104 72-73 1.2643675894221644 1104 74-75 1.2180790857006336 1104 76-77 1.1496648485666654 1104 78-79 1.123933049545986 1104 80-81 0.877766199810484 1104 82-83 0.838268437046473 1104 84-85 0.8385189888788886 1104 86-87 0.7635592682536405 1104 88-89 0.8568032733686692 1104 90-91 0.9205156083685502 1104 92-93 0.6050252371924287 1104 94-95 0.7676164598886999 1104 96-97 0.890263694239426 1104 98-99 0.7980977206867834 1104 100-101 0.2915665715118543 1104 102-103 0.7283425811422717 1104 104-105 0.8568133426845108 1104 106-107 1.0362710639343469 1104 108-109 1.0246438178175836 1104 110-111 1.029698304790326 1104 112-113 0.947726978247232 1104 114-115 0.876879947803527 1104 116-117 1.020760303854761 1104 118-119 0.9708605215722805 1104 120-121 1.0054538908082478 1104 122-123 0.7716375315507129 1104 124-125 0.928126335280961 1105 1 0.9610709437360541 1105 2 0.6686787347315182 1105 3 0.6959487334250873 1105 4 1.2883505129400739 1105 5 0.855032844368349 1105 6 0.7227439545221479 1105 7 0.6742282014467023 1105 8 0.9747533611998982 1105 9 1.3292334514830237 1105 10-11 1.0913876395534352 1105 12-13 1.1548856305714459 1105 14-15 1.3162922841477496 1105 16-17 1.0566348998695503 1105 18-19 0.7466545868565149 1105 20-21 0.9199297931941146 1105 22-23 0.9334973785020821 1105 24-25 0.9098934998873602 1105 26-27 0.7986769028833862 1105 28-29 0.39757565976150744 1105 30-31 0.754576820290275 1105 32-33 0.8156864437017077 1105 34-35 0.7318256251329416 1105 36-37 0.9849619553381608 1105 38-39 0.6325045544042638 1105 40-41 0.8327554575088705 1105 42-43 1.0210952135042746 1105 44-45 1.238628170084091 1105 46-47 1.4451102478166113 1105 48-49 1.0957801078786424 1105 50-51 1.2911001686027248 1105 52-53 1.163870746799894 1105 54-55 1.1771539068120873 1105 56-57 1.4680749632341765 1105 58-59 1.1771434584030693 1105 60-61 1.6248181265656427 1105 62-63 1.473836946640386 1105 64-65 1.264953633166499 1105 66-67 1.4527955954864957 1105 68-69 1.0995684081793513 1105 70-71 1.194643928625819 1105 72-73 1.114188976203046 1105 74-75 1.3940813673757049 1105 76-77 1.3379973905778542 1105 78-79 1.155091527429505 1105 80-81 1.1160194287134146 1105 82-83 1.1484677104772558 1105 84-85 1.099604575032945 1105 86-87 1.1682560258758912 1105 88-89 1.2715589378483614 1105 90-91 1.1776344750278263 1105 92-93 1.1912160734479045 1105 94-95 1.0348994356231636 1105 96-97 1.128547072125862 1105 98-99 0.8650919897551823 1105 100-101 0.9669220333478137 1105 102-103 0.9694449953948379 1105 104-105 1.141821721619067 1105 106-107 1.205150559582286 1105 108-109 1.2951428388268873 1105 110-111 1.2109620097201699 1105 112-113 1.2366828167965167 1105 114-115 1.1516593441442637 1105 116-117 1.308701824190674 1105 118-119 1.3412029735898194 1105 120-121 1.2642761750356968 1105 122-123 1.2943259894770272 1105 124-125 1.108842995772406 1106 1 -0.5443214029945658 1106 2 -0.35478275155675476 1106 3 -0.35422638405568563 1106 4 -0.5816379704144161 1106 5 -1.0707299720397359 1106 6 -0.6869042253455362 1106 7 -0.583367875650282 1106 8 -0.2477737078591815 1106 9 -0.1898887312521289 1106 10-11 -2.7374962003276444 1106 12-13 -0.7154628539898056 1106 14-15 -0.38706251697601957 1106 16-17 -0.6578251326201823 1106 18-19 -3.432179156759929 1106 20-21 -5.875119207318466 1106 22-23 -6.735717314327005 1106 24-25 -6.453286199245902 1106 26-27 -5.4470196977920295 1106 28-29 -1.9566556065479865 1106 30-31 -4.04161112062453 1106 32-33 -4.726912383655488 1106 34-35 -3.8052722760906335 1106 36-37 -6.169108123667371 1106 38-39 -6.660233888214513 1106 40-41 -2.8843606027346986 1106 42-43 -1.5803863273702277 1106 44-45 -0.8041372882998701 1106 46-47 -0.6336450412801078 1106 48-49 -0.43952925748581606 1106 50-51 -0.6242619285848363 1106 52-53 -0.720252311135404 1106 54-55 -0.6527479018537399 1106 56-57 -0.5136780172962503 1106 58-59 -0.4188807134964172 1106 60-61 -0.17813990978175553 1106 62-63 -0.37576859368266824 1106 64-65 -0.6502411322440871 1106 66-67 -0.8974100083171486 1106 68-69 -0.7800808021599721 1106 70-71 -0.49499674805105087 1106 72-73 -0.4945885915873447 1106 74-75 -0.5431455751573893 1106 76-77 -0.689227345698491 1106 78-79 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-1.631082649984009 2216 66-67 -2.10658400769497 2216 68-69 -2.087800494931173 2216 70-71 -4.497231415600602 2216 72-73 -4.507406008079208 2216 74-75 -2.277998924932845 2216 76-77 -1.7354053657718467 2216 78-79 -1.8325912810932152 2216 80-81 -1.9554836869405818 2216 82-83 -2.053608634216907 2216 84-85 -1.9516247776614186 2216 86-87 -2.2915750807438116 2216 88-89 -2.3908184990367403 2216 90-91 -2.5221565896144256 2216 92-93 -2.409160681792862 2216 94-95 -6.199636717041898 2216 96-97 -8.050370022948186 2216 98-99 -4.3520346017508125 2216 100-101 -3.281985630285824 2216 102-103 -4.4687940089919564 2216 104-105 -7.903063156373733 2216 106-107 -3.8088640003195238 2216 108-109 -6.796375184161292 2216 110-111 -3.794145960045281 2216 112-113 -2.9358173557298315 2216 114-115 -3.3438720251120984 2216 116-117 -2.8208461620632725 2216 118-119 -2.9418424324367294 2216 120-121 -3.5865161637896605 2216 122-123 -7.30538366388118 2216 124-125 -5.332310447972503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 129626.0 3 20718.0 4 12186.0 5 10218.0 6 10202.0 7 11251.0 8 11909.0 9 12824.0 10 14131.0 11 15048.0 12 15725.0 13 16535.0 14 17484.0 15 15104.0 16 12271.0 17 11135.0 18 10061.0 19 9522.0 20 9463.0 21 10423.0 22 12646.0 23 17514.0 24 23704.0 25 30822.0 26 37650.0 27 42990.0 28 50371.0 29 58155.0 30 66733.0 31 76847.0 32 93992.0 33 118625.0 34 152042.0 35 198614.0 36 271506.0 37 314215.0 38 102373.0 39 380.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4642240518669 19.46335099031802 13.987140938756495 23.08528401905858 2 14.56579605117063 20.612202573401014 41.49905060226349 23.322950773164862 3 18.48450355059196 26.33860765530813 29.441908676143065 25.73498011795684 4 10.738392269242022 16.812265739424458 39.332638032128 33.116703959205516 5 13.075387342151132 39.15635213732246 34.03757993036622 13.730680590160183 6 30.406141562246937 37.67623885548414 18.32517340260309 13.592446179665831 7 27.84356834886993 33.24906098270037 21.355461263977677 17.551909404452026 8 25.05238481087433 36.69567615648791 20.274547754653753 17.977391277984005 9 25.90921205452656 16.992627911241517 20.85618925362186 36.24197078061006 10-11 23.823481079642935 27.961642822628136 28.004877056316012 20.209999041412917 12-13 24.68811747190117 25.317763067328386 28.44853281157505 21.545586649195393 14-15 21.970739522477928 26.54816642752418 26.305184941749975 25.175909108247925 16-17 21.621962006480043 30.154376033675284 26.374832453815213 21.848829506029464 18-19 21.46247345209027 28.669077019200085 28.742390210561453 21.126059318148194 20-21 22.56208115387685 27.770816900194724 28.61180189456645 21.055300051361975 22-23 22.097106998458937 28.011686840029288 28.203011405624217 21.688194755887558 24-25 21.67555792916725 27.92881023365729 28.60885976649921 21.786772070676243 26-27 21.72670694156358 28.18755383916824 28.27968712826064 21.80605209100754 28-29 21.591718192825525 28.191500286564334 28.463369644523297 21.753411876086844 30-31 21.692543166892424 27.97894345638322 28.57736299310014 21.75115038362421 32-33 21.588451731594745 28.093265221690487 28.344893925212165 21.973389121502603 34-35 21.862391542351457 27.9925580831195 28.443518878312968 21.701531496216074 36-37 21.88307552375911 27.781012028396745 28.402737960906183 21.93317448693796 38-39 21.76435089936264 28.02345614833847 28.397300127100593 21.814892825198292 40-41 21.940602370270074 27.927569211142654 28.386473964009618 21.74535445457765 42-43 21.819891760558626 27.907406664756063 28.21221523079376 22.060486343891554 44-45 21.851027403546684 28.060972371282382 28.159509127376992 21.928491097793938 46-47 21.76213108204523 28.117327071351482 27.89516820355484 22.225373643048453 48-49 21.912130334933472 28.097357034150615 27.84735996838848 22.143152662527434 50-51 21.95412040471697 28.262601434770467 27.663883817727715 22.119394342784847 52-53 22.07909326009304 28.24260456891122 27.633442489544084 22.04485968145166 54-55 21.930111337999946 28.072688433812537 27.72996702137779 22.26723320680973 56-57 21.879428739653438 28.370707943050018 27.57946424292453 22.170399074372018 58-59 22.044697887615833 28.073838689996588 27.677869055901095 22.203594366486488 60-61 22.05916554883976 28.055519211745562 27.8485999752998 22.036715264114886 62-63 21.940111871505238 28.070060043641426 27.83331849929041 22.156509585562922 64-65 21.917140659300895 27.94986754613879 27.75386419530306 22.379127599257263 66-67 21.818450344747653 28.284932383099026 27.55719426905624 22.33942300309708 68-69 21.873992131935445 28.111691404033113 27.6578925040168 22.35642396001464 70-71 21.753474590900264 27.97507184888189 27.972015490115247 22.2994380701026 72-73 21.91837257622131 28.10871790157354 27.626199328214827 22.346710193990315 74-75 21.842907031415614 28.165858307622077 27.63006014954133 22.36117451142098 76-77 21.88149655106647 28.08605584313649 27.710402061780048 22.322045544017 78-79 21.94590423017662 28.098466028814133 27.64564241019149 22.309987330817755 80-81 21.781303325050313 28.28438298997064 27.590624008989966 22.34368967598908 82-83 21.90401557032933 28.331104226826053 27.512806155757087 22.25207404708753 84-85 21.934439280488853 28.076416762512814 27.564104444913895 22.42503951208444 86-87 21.8381114221793 27.970096702813063 27.704487939655486 22.487303935352152 88-89 21.975785352475796 27.903229434714444 27.656099777101616 22.464885435708144 90-91 21.846445828587804 28.118344746240293 27.75338927393764 22.28182015123426 92-93 21.88533566688501 28.039295797479795 27.633198146753113 22.442170388882083 94-95 21.821887660130905 27.925328440578728 27.572137607745212 22.680646291545155 96-97 21.92143622258555 27.93405104364331 27.5794013180109 22.56511141576024 98-99 21.699881023927738 27.899473287597882 27.59046802634198 22.810177662132407 100-101 21.620697791268988 27.973094729584187 27.699640945205328 22.706566533941498 102-103 21.89175880571558 27.822496451112393 27.640467734340273 22.645277008831755 104-105 21.781197073538795 27.724046336532282 27.759327587762417 22.73542900216651 106-107 21.578805301901905 27.85065735877031 27.814691623773474 22.75584571555431 108-109 21.6445431244647 27.92334058763512 27.46341247539418 22.968703812506007 110-111 21.562296299664986 27.984080016547864 27.64972417782694 22.803899505960214 112-113 21.645635234027154 27.99956478259913 27.60048509624906 22.754314887124654 114-115 21.52514785629461 28.058942515448226 27.526906801572927 22.889002826684234 116-117 21.294505060305067 28.08342168165654 27.73057445318582 22.891498804852574 118-119 21.454385771454156 28.05688816820089 27.435775622940213 23.05295043740475 120-121 21.397862844632378 28.077506418972533 27.597950916774273 22.926679819620823 122-123 21.500955279613056 28.025194735717385 27.539241771563233 22.934608213106326 124-125 21.51020282356072 28.14724865260496 27.27188220420912 23.070666319625197 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 708.0 1 779.0 2 1095.5 3 1336.5 4 1153.5 5 883.5 6 756.0 7 798.0 8 1001.5 9 1069.5 10 1000.0 11 955.0 12 1003.0 13 1126.0 14 1267.0 15 1380.0 16 1524.5 17 1709.0 18 1917.0 19 2235.5 20 2727.0 21 3453.5 22 4430.5 23 5761.5 24 7432.0 25 9881.5 26 13141.0 27 16700.0 28 20264.0 29 23823.5 30 28025.0 31 32620.5 32 37270.0 33 42308.5 34 47693.0 35 52298.0 36 56941.0 37 61542.0 38 64266.5 39 66248.5 40 67865.5 41 70091.0 42 72104.0 43 73635.0 44 75469.5 45 75717.0 46 75038.5 47 74504.5 48 75429.5 49 75399.0 50 72695.0 51 69514.5 52 66355.5 53 63177.0 54 59269.5 55 54229.5 56 49071.5 57 43964.0 58 39773.5 59 35553.5 60 29993.0 61 24254.5 62 19207.0 63 14967.5 64 11308.5 65 8645.5 66 6486.5 67 4970.0 68 3832.0 69 2853.5 70 2170.0 71 1656.0 72 1329.5 73 1139.0 74 952.0 75 739.0 76 470.0 77 274.5 78 187.0 79 127.0 80 83.5 81 58.5 82 55.5 83 48.5 84 33.5 85 29.0 86 22.0 87 11.0 88 9.0 89 9.5 90 9.0 91 10.0 92 8.5 93 5.0 94 4.5 95 4.5 96 2.5 97 1.5 98 2.5 99 4.5 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07975491622310839 2 0.027432561619352428 3 0.03462077295276563 4 0.0399508072067931 5 0.043618261968738616 6 0.0030806620000342296 7 0.002836165015904529 8 0.002542768634948888 9 0.006845915555631622 10-11 0.01657689552399371 12-13 0.00657696887308895 14-15 0.01021997393662149 16-17 0.021002290936741295 18-19 0.017726031349403305 20-21 0.12948560279508953 22-23 0.01574560577795273 24-25 0.015207712412867386 26-27 0.007408258619129933 28-29 0.0048410402857680754 30-31 0.002200472857167307 32-33 0.006699217365153802 34-35 0.018606220492270228 36-37 0.0030562123016212596 38-39 0.008704092635017348 40-41 0.033789483206724646 42-43 0.014131925682696705 44-45 0.10378896976305797 46-47 0.004009750539727093 48-49 0.00933978479375457 50-51 0.011149062476314354 52-53 0.011686955841399697 54-55 0.010048826047730702 56-57 0.00650361977785004 58-59 0.014058576587457794 60-61 0.024327449920905225 62-63 0.008263998063583886 64-65 0.007432708317542904 66-67 0.00909528780962487 68-69 0.008141749571519035 70-71 0.004792140888942135 72-73 7.823903492150425E-4 74-75 0.0013936328095392942 76-77 0.008801891428669228 78-79 0.014156375381109672 80-81 0.003349608682576901 82-83 0.004889939682594015 84-85 0.0034718571746417508 86-87 0.004400945714334614 88-89 0.006699217365153802 90-91 0.005281134857201536 92-93 0.003105111698447199 94-95 0.004229797825443823 96-97 0.0019315261746246362 98-99 0.00322736019051205 100-101 0.0030562123016212596 102-103 3.9119517460752126E-4 104-105 9.53538238105833E-4 106-107 6.35692158737222E-4 108-109 0.0010513370317577133 110-111 0.001858177079385726 112-113 0.002933963809556409 114-115 0.0019315261746246362 116-117 0.0038630523492492724 118-119 0.0027139165238396785 120-121 0.004914389381006985 122-123 0.00312956139686017 124-125 0.0523712540005819 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2045015.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.5298452759441 #Duplication Level Percentage of deduplicated Percentage of total 1 81.61271127071818 54.29681053391179 2 10.742709832096665 14.294216459475093 3 3.4234649593053375 6.8328778215060115 4 1.4824888857149399 3.9451902475968694 5 0.8103284979038243 2.6955514794114817 6 0.4583710540635681 1.8297213183492356 7 0.2976154583139306 1.3860217275348496 8 0.2110040434709068 1.1230453091774408 9 0.14675930026860312 0.8787486185708389 >10 0.7536901553305217 8.696246264963733 >50 0.04403002614800922 2.004475391691482 >100 0.016523567187316755 1.742883577785435 >500 7.573736958468834E-5 0.043585580902660084 >1k 2.2721210875406502E-4 0.2306256691230927 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2188 0.10699188025515706 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.889939682594015E-5 0.0 0.0 4.889939682594015E-5 0.0 6 9.77987936518803E-5 0.0 0.0 4.889939682594015E-5 0.0 7 1.4669819047782046E-4 0.0 0.0 4.889939682594015E-5 0.0 8 1.955975873037606E-4 0.0 0.0 4.889939682594015E-5 0.0 9 1.955975873037606E-4 0.0 0.0 1.4669819047782046E-4 0.0 10-11 1.955975873037606E-4 0.0 0.0 1.4669819047782046E-4 0.0 12-13 1.955975873037606E-4 0.0 0.0 1.4669819047782046E-4 0.0 14-15 2.4449698412970077E-4 0.0 0.0 1.4669819047782046E-4 0.0 16-17 2.6894668254267087E-4 0.0 0.0 2.933963809556409E-4 0.0 18-19 2.933963809556409E-4 0.0 0.0 2.933963809556409E-4 0.0 20-21 2.933963809556409E-4 0.0 0.0 5.134436666723715E-4 0.0 22-23 2.933963809556409E-4 0.0 0.0 7.823903492150425E-4 0.0 24-25 2.933963809556409E-4 0.0 0.0 0.0011491358254095935 0.0 26-27 2.933963809556409E-4 0.0 0.0 0.0013202837143003843 0.0 28-29 3.6674547619455116E-4 0.0 0.0 0.001955975873037606 0.0 30-31 4.4009457143346135E-4 0.0 0.0 0.003960851142901152 0.0 32-33 4.4009457143346135E-4 0.0 0.0 0.008752992031843288 0.0 34-35 5.134436666723715E-4 0.0 0.0 0.014474221460478286 0.0 36-37 5.867927619112818E-4 0.0 0.0 0.024547497206621957 0.0 38-39 6.35692158737222E-4 0.0 0.0 0.04310481830206624 2.4449698412970075E-5 40-41 6.35692158737222E-4 2.4449698412970075E-5 0.0 0.06652762938169157 4.889939682594015E-5 42-43 7.090412539761322E-4 4.889939682594015E-5 0.0 0.09014603804862067 4.889939682594015E-5 44-45 8.312897460409826E-4 4.889939682594015E-5 0.0 0.11818984212829736 4.889939682594015E-5 46-47 9.53538238105833E-4 4.889939682594015E-5 0.0 0.15513333643029514 4.889939682594015E-5 48-49 9.77987936518803E-4 4.889939682594015E-5 0.0 0.19789585895457978 4.889939682594015E-5 50-51 9.77987936518803E-4 4.889939682594015E-5 0.0 0.24833558678053708 4.889939682594015E-5 52-53 9.77987936518803E-4 9.77987936518803E-5 0.0 0.2954257059239174 4.889939682594015E-5 54-55 9.77987936518803E-4 9.77987936518803E-5 0.0 0.34628107862289514 4.889939682594015E-5 56-57 0.0011246861269966235 9.77987936518803E-5 0.0 0.40706302887753876 4.889939682594015E-5 58-59 0.0011246861269966235 9.77987936518803E-5 0.0 0.4825881472752034 4.889939682594015E-5 60-61 0.0011246861269966235 9.77987936518803E-5 0.0 0.5672085534824928 4.889939682594015E-5 62-63 0.0011491358254095935 9.77987936518803E-5 0.0 0.656938946658093 4.889939682594015E-5 64-65 0.0011735855238225637 9.77987936518803E-5 0.0 0.7444688669765258 4.889939682594015E-5 66-67 0.0012224849206485039 9.77987936518803E-5 0.0 0.848135588247519 4.889939682594015E-5 68-69 0.0012224849206485039 9.77987936518803E-5 0.0 0.9720955592012772 4.889939682594015E-5 70-71 0.0012469346190614738 9.77987936518803E-5 0.0 1.1109942958853603 4.889939682594015E-5 72-73 0.001295834015887414 9.77987936518803E-5 0.0 1.2626313254426007 4.889939682594015E-5 74-75 0.001320283714300384 9.77987936518803E-5 0.0 1.4135104143490391 4.889939682594015E-5 76-77 0.0013691831111263242 9.77987936518803E-5 0.0 1.5780324349699146 4.889939682594015E-5 78-79 0.0014180825079522644 9.77987936518803E-5 0.0 1.772285288860962 4.889939682594015E-5 80-81 0.001613680095256025 9.77987936518803E-5 0.0 2.0013300635936657 4.889939682594015E-5 82-83 0.0017114788889079054 9.77987936518803E-5 0.0 2.245338053755107 4.889939682594015E-5 84-85 0.0017359285873208754 9.77987936518803E-5 0.0 2.4785148275196027 4.889939682594015E-5 86-87 0.0017603782857338454 9.77987936518803E-5 0.0 2.720811338792136 4.889939682594015E-5 88-89 0.0017603782857338454 9.77987936518803E-5 0.0 3.0053813786206947 4.889939682594015E-5 90-91 0.0017848279841468154 9.77987936518803E-5 0.0 3.328655291036985 4.889939682594015E-5 92-93 0.0018581770793857258 9.77987936518803E-5 0.0 3.684129456263157 4.889939682594015E-5 94-95 0.0018581770793857258 9.77987936518803E-5 0.0 4.0305572330765305 4.889939682594015E-5 96-97 0.0018581770793857258 9.77987936518803E-5 0.0 4.375860323762906 4.889939682594015E-5 98-99 0.002004875269863546 9.77987936518803E-5 0.0 4.7529969217829695 4.889939682594015E-5 100-101 0.002127123761928397 9.77987936518803E-5 0.0 5.193751635073582 4.889939682594015E-5 102-103 0.002249372253993247 9.77987936518803E-5 0.0 5.671596540856669 4.889939682594015E-5 104-105 0.0024938692381229477 9.77987936518803E-5 0.0 6.148341210211172 4.889939682594015E-5 106-107 0.0026405674286007685 9.77987936518803E-5 0.0 6.618631159184652 4.889939682594015E-5 108-109 0.0026894668254267085 1.2224849206485039E-4 0.0 7.105498003682125 4.889939682594015E-5 110-111 0.0026894668254267085 1.4669819047782046E-4 0.0 7.656765353799361 4.889939682594015E-5 112-113 0.0026894668254267085 1.4669819047782046E-4 0.0 8.255709615821889 4.889939682594015E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5565 0.0 55.19593 1 GGTATCA 2940 0.0 52.84643 1 ATCAACG 6620 0.0 44.581528 3 TCAACGC 6870 0.0 43.32048 4 CAACGCA 6875 0.0 43.287914 5 AACGCAG 6770 0.0 42.445984 6 TATCAAC 7455 0.0 41.184456 2 GTACATG 9535 0.0 35.96038 1 ACGCAGA 8045 0.0 34.683662 7 TACATGG 9730 0.0 33.817657 2 CGCAGAG 7895 0.0 33.68559 8 ACATGGG 9735 0.0 30.25523 3 GCAGAGT 8715 0.0 28.331495 9 CATGGGA 6950 0.0 26.46389 4 CGCATAG 240 1.3040335E-8 24.790056 8 ATGGGAG 3290 0.0 24.061636 5 AGAGTAC 8060 0.0 23.325172 10-11 CAGAGTA 8510 0.0 23.210331 10-11 GAGTACA 7290 0.0 23.027359 1 AGTACAT 7050 0.0 22.197184 2 >>END_MODULE