FastQCFastQC Report
Thu 26 May 2016
SRR1364486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364486_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045015
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61390.3001933971144466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60830.29745503089219394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43080.21065860152615018No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46300.064.6657261
GTATCAA82350.050.480141
ATCAACG104800.037.6357963
TATCAAC110200.037.4676132
TCAACGC106200.036.8591424
CAACGCA107600.036.270895
AACGCAG110850.034.9927866
GTACATG90750.029.4430481
ACGCAGA131300.029.3621277
CGCAGAG132300.029.0866858
TACATGG92950.027.691012
GCAGAGT139650.026.2781039
GTGGTAT24250.025.8243241
TGGTATC23450.025.4074762
ACATGGG94550.025.3319683
GAGTACT92800.024.35544412-13
AGTACTT90600.024.0934112-13
CAGAGTA138450.023.95037510-11
GTACTTT96150.023.75430714-15
AGAGTAC133100.023.59479910-11
Produced by FastQC (version 0.11.5)